JP2018529326A5 - - Google Patents
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- JP2018529326A5 JP2018529326A5 JP2018508166A JP2018508166A JP2018529326A5 JP 2018529326 A5 JP2018529326 A5 JP 2018529326A5 JP 2018508166 A JP2018508166 A JP 2018508166A JP 2018508166 A JP2018508166 A JP 2018508166A JP 2018529326 A5 JP2018529326 A5 JP 2018529326A5
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- nickase
- nucleic acid
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- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims description 182
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 150
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 150
- 150000007523 nucleic acids Chemical group 0.000 claims description 108
- 239000000523 sample Substances 0.000 claims description 82
- 108020004414 DNA Proteins 0.000 claims description 57
- 101710163270 Nuclease Proteins 0.000 claims description 53
- 102000039446 nucleic acids Human genes 0.000 claims description 44
- 108020004707 nucleic acids Proteins 0.000 claims description 44
- 125000003729 nucleotide group Chemical group 0.000 claims description 33
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 27
- 102000053602 DNA Human genes 0.000 claims description 22
- 239000002773 nucleotide Substances 0.000 claims description 21
- 108020005004 Guide RNA Proteins 0.000 claims description 16
- 230000008685 targeting Effects 0.000 claims description 16
- 108091033409 CRISPR Proteins 0.000 claims description 15
- 108091092584 GDNA Proteins 0.000 claims description 15
- 108091092878 Microsatellite Proteins 0.000 claims description 14
- 230000000295 complement effect Effects 0.000 claims description 12
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- 101150055601 cops2 gene Proteins 0.000 claims description 9
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- 230000037430 deletion Effects 0.000 claims description 7
- 238000012217 deletion Methods 0.000 claims description 7
- 230000001105 regulatory effect Effects 0.000 claims description 7
- 108010017826 DNA Polymerase I Proteins 0.000 claims description 6
- 102000004594 DNA Polymerase I Human genes 0.000 claims description 6
- 230000000977 initiatory effect Effects 0.000 claims description 6
- 230000005782 double-strand break Effects 0.000 claims description 5
- 238000001668 nucleic acid synthesis Methods 0.000 claims description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 3
- 108090000364 Ligases Proteins 0.000 claims description 3
- 102000003960 Ligases Human genes 0.000 claims description 3
- 108010006785 Taq Polymerase Proteins 0.000 claims description 3
- 230000006820 DNA synthesis Effects 0.000 claims description 2
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- 239000000203 mixture Substances 0.000 description 29
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- 238000012163 sequencing technique Methods 0.000 description 7
- 238000010354 CRISPR gene editing Methods 0.000 description 6
- 102000008579 Transposases Human genes 0.000 description 6
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- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 4
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021193224A JP7792241B2 (ja) | 2015-08-19 | 2021-11-29 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562207359P | 2015-08-19 | 2015-08-19 | |
| US62/207,359 | 2015-08-19 | ||
| PCT/US2016/047631 WO2017031360A1 (en) | 2015-08-19 | 2016-08-18 | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021193224A Division JP7792241B2 (ja) | 2015-08-19 | 2021-11-29 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018529326A JP2018529326A (ja) | 2018-10-11 |
| JP2018529326A5 true JP2018529326A5 (cg-RX-API-DMAC7.html) | 2019-09-26 |
| JP6986509B2 JP6986509B2 (ja) | 2021-12-22 |
Family
ID=58050956
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018508166A Active JP6986509B2 (ja) | 2015-08-19 | 2016-08-18 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
| JP2021193224A Active JP7792241B2 (ja) | 2015-08-19 | 2021-11-29 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021193224A Active JP7792241B2 (ja) | 2015-08-19 | 2021-11-29 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10538758B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP3985111A1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP6986509B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN108138176B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2016308283B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2995983A1 (cg-RX-API-DMAC7.html) |
| DK (1) | DK3337898T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2017031360A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (59)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US20150166982A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting pi3k point mutations |
| CA2939621C (en) | 2014-02-13 | 2019-10-15 | Takara Bio Usa, Inc. | Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same |
| EP3177718B1 (en) | 2014-07-30 | 2022-03-16 | President and Fellows of Harvard College | Cas9 proteins including ligand-dependent inteins |
| CN114438169B (zh) | 2014-12-20 | 2024-11-08 | 阿克生物公司 | 使用CRISPR/Cas系统蛋白靶向消减、富集、和分割核酸的组合物及方法 |
| CN108138176B (zh) | 2015-08-19 | 2022-05-24 | 阿克生物公司 | 使用基于核酸引导的核酸酶的系统捕获核酸 |
| SG11201803173VA (en) | 2015-10-23 | 2018-05-30 | Harvard College | Nucleobase editors and uses thereof |
| EP3386550B1 (en) | 2015-12-07 | 2021-01-20 | Arc Bio, LLC | Methods for the making and using of guide nucleic acids |
| JP7231935B2 (ja) | 2016-08-03 | 2023-03-08 | プレジデント アンド フェローズ オブ ハーバード カレッジ | アデノシン核酸塩基編集因子およびそれらの使用 |
| CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| EP3507382B1 (en) * | 2016-09-02 | 2021-06-23 | New England Biolabs, Inc. | Analysis of chromatin using a nicking enzyme |
| WO2018071868A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
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| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
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| KR102634727B1 (ko) * | 2017-06-14 | 2024-02-07 | 위스콘신 얼럼나이 리서어치 화운데이션 | 변형된 가이드 rna, crispr-리보뉴클레오프로테인 복합체 및 사용 방법 |
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| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
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| US11279926B2 (en) | 2015-06-05 | 2022-03-22 | The Regents Of The University Of California | Methods and compositions for generating CRISPR/Cas guide RNAs |
| CN108138176B (zh) | 2015-08-19 | 2022-05-24 | 阿克生物公司 | 使用基于核酸引导的核酸酶的系统捕获核酸 |
| EP3360067B1 (en) | 2015-10-07 | 2025-10-01 | The Board of Trustees of the Leland Stanford Junior University | Techniques for determining whether an individual is included in ensemble genomic data |
| CN108778343A (zh) | 2015-10-16 | 2018-11-09 | 天普大学-联邦高等教育系统 | 利用cpf1进行rna向导的基因编辑的方法和组合物 |
| EP3433382B1 (en) | 2016-03-25 | 2021-09-01 | Karius, Inc. | Synthetic nucleic acid spike-ins |
| US11046995B2 (en) | 2016-08-16 | 2021-06-29 | The Regents Of The University Of California | Method for finding low abundance sequences by hybridization (FLASH) |
| US20180051320A1 (en) | 2016-08-22 | 2018-02-22 | The Regents Of The University Of California | Depletion of abundant sequences by hybridization (dash) |
| WO2018069430A1 (en) | 2016-10-13 | 2018-04-19 | bioMérieux | Identification and antibiotic characterization of pathogens in metagenomic sample |
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2016
- 2016-08-18 CN CN201680057464.3A patent/CN108138176B/zh active Active
- 2016-08-18 AU AU2016308283A patent/AU2016308283B2/en active Active
- 2016-08-18 EP EP21188035.6A patent/EP3985111A1/en active Pending
- 2016-08-18 DK DK16837863.6T patent/DK3337898T3/da active
- 2016-08-18 WO PCT/US2016/047631 patent/WO2017031360A1/en not_active Ceased
- 2016-08-18 US US15/520,052 patent/US10538758B2/en active Active
- 2016-08-18 EP EP16837863.6A patent/EP3337898B1/en active Active
- 2016-08-18 JP JP2018508166A patent/JP6986509B2/ja active Active
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- 2023-02-01 US US18/163,196 patent/US20240132872A1/en active Pending
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