JP2018093836A - Plant cultivation method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide cultivation methods of leaf vegetables in which the yield is high while the content of folic acid in a plant to be cultivated can be increased, without requiring pH adjustment nor sterilization step.SOLUTION: A plant cultivation method of cultivating leaf vegetables by supplying a nutrient solution is provided, the nutrient solution is characterized by that phenylalanine is added. The loadings of phenylalanine is 0.001 to 2 parts by mass with respect to 100 parts by mass of a nutrient solution. A planting panel plate in which a lot of planting holes are drilled is arranged on a cultivation bed having inclination, a hydrophilic sheet is arranged to the upper surface side of the bottom face of the cultivation bed, the nutrient solution is supplied to the upper surface side of the bottom face of the cultivation bed and leaf vegetables are cultivated.SELECTED DRAWING: Figure 3

Description

  The present invention relates to a plant cultivation method, and more particularly to a plant cultivation method for leaf vegetables containing folic acid.

  In a study conducted by the Ministry of Health, Labor and Welfare in 1999, it became clear that lack of folic acid tends to increase the incidence of neural tube damage in infants, and the need for its intake has begun to be recognized. In response to this, folic acid intake has been promoted by the country, and its importance is also described in maternal and child handbooks. As described above, folic acid is a nutrient that needs to be ingested, and the Japanese dietary intake standard (2015 version) established by the Ministry of Health, Labor and Welfare is established, and the current recommended amount is 240 μg per day for adult women. In addition, it is recommended that pregnant women take 240 μg and lactating women take 100 μg.

  In order to consume this folic acid in a daily diet, many supplements are commercially available and can be easily consumed, but consumption that you want to take from food in your daily diet There are many people. Moreover, foodstuffs, such as vegetables, contain abundant nutrients other than folic acid, and it is important to ingest nutrients through foodstuffs in daily meals. Because of these facts, attempts have been made in recent years to make edible plants contain a large amount of folic acid.

  For example, Patent Document 1 (Japanese Patent Application Laid-Open No. 2011-182672) discloses an edible aqueous solution containing folic acid at a concentration of 0.1 to 1% by mass at a temperature in the range of 10 to 25 ° C. and further adjusting the pH. A method for obtaining an edible plant body containing a high concentration of folic acid by immersing a part of the plant body is disclosed.

  However, the method of Patent Document 1 requires a delicate pH adjustment in order to prepare a high-concentration folic acid aqueous solution having a high concentration of preferably about 0.75% by mass. There is a problem that the plant is stressed, the plant is stressed, the ability of the root to be absorbed is inhibited, the plant suffers from wilt and root rot due to a microelement deficiency symptom, and the plant is weakened and sometimes die.

  Patent Document 2 (Japanese Patent No. 576282) discloses a method for cultivating a plant by using yeast as a folic acid supply source and immersing the root of the plant in a liquid medium containing yeast extract at a specified concentration. Is disclosed.

  However, in order to prevent the propagation of various bacteria contained in the liquid medium containing yeast, a sterilization treatment is necessary, and in order to perform this sterilization treatment, a heating device is necessary, and further, the liquid medium is accommodated in the culture container. Therefore, a cooling device is required. For this reason, there is a problem that equipment costs are required, and if sterilization is insufficient, various germs grow, inhibit root growth of the plant body, cause root rot, and eventually cause poor growth.

JP 2011-182672 A Japanese Patent No. 576282

  An object of the present invention is to provide a plant cultivation method that can increase the folic acid content in leafy vegetables to be cultivated without requiring pH adjustment or a sterilization step, and has a high yield.

  As a result of intensive studies in view of the above problems, the present inventors have found that the above problems can be solved by including phenylalanine in the nutrient solution in the method of cultivating leafy vegetables by supplying the nutrient solution, thereby completing the present invention. I came to let you.

  That is, the plant cultivation method of the present invention is a method for cultivating leafy vegetables by supplying a nutrient solution, wherein the nutrient solution is added with phenylalanine.

  Phenylalanine used in the present invention is one of amino acids contained in proteins in foods, and is abundant in foods such as bonito, koya tofu, soybeans and liver. In addition, aspartame, which is an artificial sweetener formed by combining phenylalanine and aspartic acid, is used in many foods as a food additive.

  In 1 aspect of this invention, the said phenylalanine is added 0.001-2 mass part with respect to 100 mass parts of nutrient solution.

  In one aspect of the present invention, a fixed planting panel plate having a large number of planting holes is disposed on a cultivation bed having a gradient, and a seedling root pot is placed on the cultivation bed through the planting hole. The nutrient solution is supplied to the upper surface side of the bottom surface of the cultivation bed to cultivate leafy vegetables.

  In one aspect of the present invention, a plurality of ridges extending in the gradient direction are provided on the upper surface of the cultivation bed, and the ridges are located below the planting holes, The space is a groove, and the seedling pot is placed on the ridge, and the nutrient solution is poured into the groove.

  In one aspect of the present invention, a hydrophilic sheet is disposed on the upper surface side of the bottom surface of the cultivation bed.

  In one embodiment of the present invention, the leafy vegetables are Ominae, Brassicaceae, Amaryllidaceae, Apiaceae, Lamiaceae, Amaranthaceae, Asteraceae, or Rubiaceae.

  According to the plant cultivation method of the present invention, by adding phenylalanine to the nutrient solution, it is possible to increase the folic acid content in the plant to be cultivated without requiring pH adjustment or a sterilization step, and a plant with a high yield. It becomes possible to provide a cultivation method.

It is a perspective view of a cultivation bed. It is sectional drawing of the convex part of FIG. It is sectional drawing of the cultivation bed during plant cultivation. It is a top view explaining cultivation facilities.

  Hereinafter, the present invention will be described in more detail. However, the present invention is not limited to the following embodiments as long as the effects of the present invention are achieved.

  The leafy vegetables cultivated by the method of the present invention are preferably Ominaceae, Brassicaceae, Amaryllidaceae, Apiaceae, Lamiaceae, Amaranthaceae, Asteraceae, or Rubiaceae, and spinach is particularly preferred.

  In the plant cultivation method of the present invention, the nutrient solution is added with phenylalanine.

  Folic acid has a structure in which pteridine, paraaminobenzoic acid and glutamic acid are bound. In previous studies, the synthesis of folic acid is known to involve three organs in the cell, the mitochondria, cytoplasmic matrix, and chloroplast, and pteridine produced from guanosine triphosphate in the cytoplasmic matrix. Then, the paraaminobenzoic acid produced in the chloroplast moves into the mitochondria, then becomes pteroic acid, further binds to glutamic acid, and becomes pteroyl monoglutamic acid (folic acid).

  Folic acid refers to pteroyl monoglutamic acid in a narrow sense, but is a generic name including a polyglutamic acid type in a broad sense.

  Most of folic acid in food exists as a polyglutamic acid type and exists in a state of being bound to an enzyme protein.

  Folic acid is produced in the plant by the process described above, and is called GTP cyclohydrolase I (hereinafter referred to as GCHI) involved in the synthesis of pteridine, which is one of the substances constituting folic acid. It has been reported in previous studies that the enzyme affects the amount of folic acid synthesis. This enzyme called GCHI synthesizes dihydroneopterin triphosphate, which is an intermediate of folic acid synthesis, from guanosine triphosphate (hereinafter referred to as GTP). Therefore, it is considered that there is an effect of increasing the amount of folic acid in the plant body by activating this GCHI.

  On the other hand, recent studies have revealed that this GCHI forms a complex with a protein called GCHI Feedback Regulated Protein (hereinafter referred to as GFRP) and that there is a feedback regulation mechanism. It has been clarified that the action of GCHI is activated by the binding of phenylalanine to the body. However, although it is clear that this GFRP exists in the living body of mammals, it is not clear in the present research that it exists in the plant body.

  However, when cultivation using phenylalanine was performed by the method defined in the present invention, a result of increasing folic acid was obtained. The mechanism for increasing folic acid is not clear, but it is presumed that the addition of phenylalanine is effective for the activation of GCHI by some mechanism.

  The method of adding the phenylalanine to the nutrient solution is not particularly limited, and a prescribed amount of phenylalanine may be added to the nutrient solution during cultivation, and the phenylalanine is added to the liquid fertilizer stock solution in advance and stirred and mixed. Later, it can be added to the cultivated nutrient solution.

  In addition, phenylalanine may be added to the cultivation nutrient solution by adding phenylalanine (powder) to the cultivation nutrient solution in a state in which phenylalanine is previously dissolved in the cultivation nutrient solution or water to obtain a phenylalanine aqueous solution. . The latter addition method is preferred in terms of making the phenylalanine distribution in the cultivation nutrient solution more uniform.

  As the phenylalanine, a commercially available product can be used. For example, “L (−)-Phenylalanine” sold by Wako Pure Chemical Industries, “L-Phenylalanine” manufactured by Toronto Research Chemicals, The United, “L-Phenylalanine” manufactured by States Pharmaceutical Covention can be used.

  There is no particular limitation on the timing of adding phenylalanine to the nutrient solution during the cultivation period of the plant, and phenylalanine may be always contained during the cultivation period. Phenylalanine may be contained in the solution, and the cultivation may be performed using only a normal nutrient solution during other periods. For a certain period, the period in which phenylalanine is contained may be contained multiple times at intervals, and it is particularly preferable to add it in the latter stage of cultivation.

  In the plant cultivation method of this invention, it is preferable to add 0.001 mass part or more of phenylalanine with respect to 100 mass parts of nutrient solution, It is more preferable to add 0.003 mass part or more, 0.006 mass part or more More preferably, it is added. By setting the added amount of phenylalanine as described above, the folic acid concentration in the plant to be cultivated can be increased efficiently.

  Although the upper limit of the addition amount of phenylalanine is not particularly limited, it is preferable to add 2 parts by mass or less of phenylalanine to 100 parts by mass of the nutrient solution, and it is more preferable to add 1 part by mass or less. More preferably, it is more preferably added in an amount of 0.1 parts by mass or less.

  In the plant cultivation method of the present invention, it is preferable to cultivate leafy vegetables using a cultivation apparatus that cultivates by circulating a nutrient solution.

  In this cultivation apparatus, a fixed planting panel plate having a large number of planting holes is arranged on a cultivation bed having a gradient, and a seedling root pot is placed on the cultivation bed through the planting hole. The nutrient solution is supplied to the upper surface side of the bottom surface of the cultivated leaf vegetables.

  Preferably, a plurality of ridges extending in the gradient direction are provided on the upper surface of the cultivation bed, the ridges are located below the planting holes, and the ridges are between the ridges. The seedling pots are placed on the ridges, and the nutrient solution is poured into the ridges. Furthermore, it is preferable that a hydrophilic sheet is disposed on the upper surface side of the bottom surface of the cultivation bed, and a nutrient solution is supplied to the upper surface side of the bottom surface of the cultivation bed to cultivate leafy vegetables.

  By using such a cultivation device, the nutrient solution can be circulated at a certain flow rate on the upper surface side of the bottom of the cultivation bed, the phenylalanine distribution in the nutrient solution becomes uniform, and is sufficient for the root of the plant body And uniformly supplied. Thereby, phenylalanine can be efficiently supplied to the root of the plant.

  Moreover, the said cultivation apparatus may be provided with the temperature adjustment means to hold | maintain the nutrient solution to supply in the preset temperature range, and the density | concentration adjustment means of the said nutrient solution.

  The temperature adjusting means can maintain the temperature of the circulating nutrient solution within a preset range throughout the year. The temperature adjusting means includes a temperature sensor that detects the temperature in the nutrient solution tank, a heat exchanger that is disposed in the nutrient solution tank and exchanges heat with the nutrient solution, and a heat medium that supplies the heat medium to the heat exchanger A supply line (temperature adjustment line) and a control valve that is interposed in the heat medium supply line and controls the supply amount of the heat medium to the heat exchanger by a detection signal from the temperature sensor can be used.

  The concentration adjusting means includes a plurality of nutrient solution stock tanks for storing nutrient solutions of different types and concentrations, a transfer line for feeding the nutrient solution stock solution in each stock solution tank to the nutrient solution tank by a pump, By comprising the three-way switching valve (open / close valve) or the like interposed in these transfer lines, the concentration of the circulating nutrient solution can be adjusted.

  It is preferable that a ridge is formed on the upper surface of the cultivation bed under the planting hole of the fixed planting panel. The width of the ridge is determined by the diameter of the seedling pot used. If the width of the ridge is narrower than the diameter of the seedling basin, the seedling basin may be displaced from the bowl-shaped convex part and tilted. Preferably, the width of the ridge is larger than the diameter of the seedling pot used, and more preferably smaller than the width of 4 mm added to the diameter of the seedling pot.

  The nutrient solution flowing on the upper surface of the cultivation bed flows through the grooves between the projections of the cultivation bed. The seedling root pot inserted into the planting hole is placed on the upper surface of the ridge. Since the seedling root pot is not washed by the flow of the nutrient solution, the culture medium of the seedling root pot is prevented from collapsing or flowing out of the culture medium.

  According to this cultivation bed, it is possible to generate roots having two different forms and functions: an aquatic root that grows in water and a humid root that is maintained in moisture and has many root hairs. Underwater roots mainly absorb fertilizer and water in the nutrient solution, and wet roots mainly absorb oxygen directly from the moisture.

  According to this cultivation method, it is possible to cultivate a plant without relying only on dissolved oxygen in the nutrient solution, and the roots of the plant do not fall into oxygen deficiency even in cultivation in the high temperature period where dissolved oxygen tends to be insufficient.

  The suitable structure of this cultivation bed is demonstrated with reference to FIGS. Moreover, the cultivation apparatus provided with this cultivation bed is shown in FIG.

  As shown in FIGS. 1 to 3, a large number of planting holes 52 are drilled in the fixed planting panel 51 formed of lightweight foamed polystyrene. The size of the planting panel 51 is 600 mm wide, 1000 mm deep, and 35 mm thick, for example. Although the shape of the planting hole 52 may be an inverted conical shape, it is better to use a cylindrical shape having the same upper and lower diameters, and the size is larger than the diameter of the seedling pot 54 used. The interval between the planting holes 52 is determined as an appropriate interval for crop cultivation. For example, in the case of spinach, assuming that the size of the fixed planting panel 51 is as described above, a total of 45 cylindrical planting holes 52 having a diameter of 27 mm are arranged in a diamond shape at intervals of 118 mm.

  The cultivation bed 53 on which the above-described planted panel plates 51, 51 are placed on the upper surface is molded by a lightweight foamed polystyrene, like the planted panel plate 51. In the example shown in the drawing, the two fixed planting panel plates 51 and 51 are supported by stepped portions 59 and 59 formed on both sides of the cultivation bed 53 and a receiving portion 60 formed in the center of the upper surface. An example of the size of the cultivation bed 53 is as follows: width 1260 mm, depth 1000 mm, and side wall height 100 mm.

  A plurality of ridges 56 that are continuous in the longitudinal direction are formed on the bottom surface of the cultivation bed 53 that is directly below the planting hole 52 of the planting panel 51. The nutrient solution L flows down into the groove 55 between the protrusions 56 and 56. The height of the ridges 56 is determined by the relationship with the depth of the nutrient solution L, and the width of the ridges 56 is determined by the diameter of the seedling pot 54 used. If the height of the ridges 56 is too low, it is not preferable because the seed pot 54 placed on the ridges 56 may be washed with the nutrient solution L. It is not preferable because the distance from the liquid surface of L is too far, and the water supply to the seedling root pot 54 is insufficient and the growth is delayed. If the width of the ridge 56 is narrower than the diameter of the seedling basin 54, the seedling basin 54 may fall off the hook-like ridge 56 and tilt. Desirably, the height of the ridge 56 is about 2 to 3 mm higher than the depth of the nutrient solution L, and the width of the ridge 56 is larger than the diameter of the seedling root pot 54 to be used. It is more preferable that the width is smaller than the width of 4 mm added to the diameter. The interval between the ridges 56 is equal to the interval between the planting holes 52.

  Preferably, a plurality of cultivation beds 53 are continuously provided in the longitudinal direction and installed so as to have a gradient of about 1/50 to 1/150. In this case, as shown in FIG. 2, the entire upper surface of the cultivation bed 53 provided continuously is covered with a plastic sheet 57 to prevent water leakage at each connection place. A hydrophilic sheet 58 is preferably laid. This hydrophilic sheet 58 is for pumping up the liquid by capillary action.

  As shown in FIG. 3, the planting panel 53 is placed on the cultivation bed 53, and the seedling pot 54 is dropped from the planting hole 52. The seedling root pot 54 is placed on the ridge 56 of the cultivation bed 53 facing directly below the planting hole 52. Next, the nutrient solution L is allowed to flow from the upstream side to the downstream side of the cultivation bed 53 through the recess 55. When the flow rate of the nutrient solution L is 10 liters / minute per cultivation bed, the liquid level in the groove is approximately 2 to 3 mm. This is about half the height of the ridge 56. A moisture space having a height of about 25 mm is formed between the lower surface of the planting panel 51 and the liquid surface of the nutrient solution L in the groove.

  In the above description, the cultivation bed 53 is made of foamed synthetic resin, but using various plate-like or plate-like members in which convex and concave stripes are alternately formed in parallel, such as a synthetic resin corrugated plate. Also good.

  As illustrated in FIG. 4, the cultivation apparatus that can be used in the present invention has a parent tank 86 that stores diluted nutrient solution, and at least one or more child tanks 73 that supply the nutrient solution from the parent tank 86. It is preferable that at least one cultivation bed 53 to which the nutrient solution is supplied from the child tank 73 is disposed.

  A liquid fertilizer stock solution in a stock solution tank (not shown) and water such as tap water are supplied to the parent tank 86 from pipes 84 and 85 with supply control valves 84a and 85a to prepare a nutrient solution having a predetermined concentration. A nutrient solution having a predetermined concentration prepared in the parent tank 86 is distributed and supplied to each child tank 73 via a pump 87, a pipe 88, a three-way valve 89, a flow meter 90 and a ball tap 91. A water supply pipe 92 is connected to the three-way valve 89, and water from the pipe 92 can be supplied to the child tank 73 by switching the three-way valve 89. The liquid in each child tank 73 is supplied to each cultivation bed 53 via a pump 74 and a pipe 75.

  In FIG. 4, a plurality of the cultivation beds 53 are arranged and leaf vegetables are cultivated. The nutrient solution prepared in the parent tank 86 is supplied to the plurality of cultivation beds 53 through the child tank 73. Thereby, the nutrient solution (dilution nutrient solution) of the uniform density | concentration prepared with the parent tank 86 can always be supplied to each cultivation bed 53. FIG.

  In FIG. 4, a cultivation bet group 62 is formed by arranging a plurality of cultivation bet rows 61 (four rows in the drawing) in which a plurality of cultivation bets 53 are arranged in one row with a gradient. One child tank 73 is attached to one cultivation bed group 62.

  In the cultivation apparatus of FIG. 4, phenylalanine is preferably added to the child tank 73.

  As shown in FIG. 4, by providing the child tank 73 for each cultivation bed group 62, it is possible to manage the nutrient solution cultivated in the child tank 73 with a relatively small amount. When the harvesting is completed, it is preferable that the nutrient solution used for one cultivation bed group 62 is discarded and cultivation is started with a new nutrient solution.

  As a result, the vegetables that are cultivated in the next period without being affected by secretions from the roots (organic acids, etc.) or detachment of the epidermis cells of the roots that have flowed into the nutrient solution due to cultivation of the first crop, Stable cultivation becomes possible.

  In the conventional method, since the nutrient solution is supplied to each cultivation bed by a common tank and cultivated, the nutrient solution used is to reuse the nutrient solution while adding a new nutrient solution each time. As a result, the secretion from the roots and the epidermis cells of the roots accumulate, and as the cultivation is repeated, growth inhibition called self-poisoning occurs.

Moreover, even in the case of the conventional method, it is possible to renew all of the nutrient solution, but since it becomes an operation to replace the nutrient solution in the tank and all the cultivation beds at the same time, it is necessary to discard a large amount of the nutrient solution at the same time, Furthermore, during this work, all vegetables cannot be grown.
As a result, during this time, vegetables cannot be shipped, and there is a problem that regular vegetables cannot be shipped.

  In FIG. 4, the nutrient solution used in one cultivation bed group 62 is returned to the child tank 73 that has supplied the nutrient solution to each cultivation bed 53 of the cultivation bed group 62 via the pipe 76 to circulate the nutrient solution. . The nutrient solution is additionally supplied from the parent tank 86 into the child tank 73 by the ball tap 91 or the like, and the nutrient solution in the child tank 73 is kept constant.

  In FIG. 4, while the cultivation is continued in some of the cultivation bed groups 62, the other cultivation bed groups 62 perform cleaning (cleaning after the harvesting is finished). The process can proceed separately.

  Further, even when a pathogenic bacterium is generated in one cultivated bed group 62, the infection of the pathogenic bacterium in the other cultivated bed group 62 can be suppressed. That is, since the nutrient solution is not returned to the parent tank 86, the contamination stops only in the closed circuit (cultivation bed group 62) for circulating the nutrient solution.

  Water can be introduced into each child tank 73 via a water supply pipe 92 and a three-way valve 89. By switching from supplying nutrient solution to supplying water at the later stage of cultivation of leaf vegetables grown in each cultivation bed group 62, the fertilizer concentration of the nutrient solution circulating through the child tank 73 and the cultivation bed 53 can be reduced. . As a result, it is possible to gradually reduce the amount of nitric acid in the plant in the latter stage of cultivation, and leaf vegetables can be harvested with the amount of nitric acid reduced.

  When nitric acid in a plant body is taken into the human body, it combines with amide nitrogen to produce nitrosamine. By lowering the fertilizer concentration of the nutrient solution in the later stage of cultivation, the nitric acid concentration in the plant body can be reduced. In addition, by reducing the concentration of nitrogen, phosphoric acid, and potash in the nutrient solution used in the later stages of cultivation, the burden on the environment can be greatly reduced even when the nutrient solution is discarded after harvesting is completed. Can do.

[Basic conditions]
As a basic condition, the cultivation bed shown in FIGS. 1 to 3 having a gradient of 1/100 has a fixed planting panel plate having 270 planting holes, and a hydrophilic sheet is provided on the upper surface side of the bottom surface of the cultivation bed. Then, a nutrient solution (nutrient concentration: EC 3.0 dS / m, nutrient solution temperature: 20 ° C.) was supplied to the bottom of the cultivation bed at a flow rate of 10 liters per minute to grow spinach.

  A seedling root pot having a spinach seedling was planted in the cultivation bed, and the plant was cultivated for 15 days after the planting.

<Comparative Example 1>
Spinach was cultivated under the above basic conditions, and the content of harvested spinach folic acid was measured. In addition, the value of folic acid content is obtained by using a liquid chromatography and a mass spectrometer, samples of three strains extracted from the spinach group cultivated under the same conditions, monoglutamic acid type folic acid, dihydrofolic acid, tetrahydrofolic acid, 5-formyl Tetrahydrofolic acid, 10-formyltetrahydrofolic acid, and 5-methyltetrahydrofolic acid were each measured, and the total was defined as the “folic acid” content. The results are shown in Table 1.

  In addition, the measurement result of Table 1 makes content of the spinach folic acid harvested by cultivation of the basic conditions of the comparative example 1 100%, and the spinach folic acid cultivated by Examples 1 to 3 and Reference Example 1 described later. The content is shown as a ratio to the content of Comparative Example 1.

<Example 1>
Compared with the cultivation method similar to Comparative Example 1 except that 0.008 parts by mass of phenylalanine was added to 100 parts by mass of the nutrient solution for 10 days and 14 days from planting the spinach seedlings twice in total. Spinach was cultivated simultaneously with Example 1, and the content of spinach folic acid on the 15th day after planting was measured. The obtained results are shown in Table 1.

<Example 2>
Spinach at the same time as Comparative Example 1 except that 0.017 parts by mass of phenylalanine was added to 100 parts by mass of nutrient solution once on the 14th day after the planting of spinach seedlings. The content of spinach folic acid on the 15th day after planting was measured. The obtained results are shown in Table 1.

<Example 3>
Except that 0.017 parts by mass of phenylalanine was added to 100 parts by mass of the nutrient solution for 5 times in total on the first day, the third day, the seventh day, the tenth day, and the 14th day from the planting of spinach seedlings, Spinach was cultivated simultaneously with Comparative Example 1 by the same cultivation method as Comparative Example 1, and the content of spinach folic acid on the 15th day after cultivated planting was measured. The obtained results are shown in Table 1.

<Reference Example 1>
Compared with the cultivation method similar to Comparative Example 1 except that spinach seedlings were added twice in total on the 10th and 14th day after planting, and 0.075 parts by mass of methionine was added to 100 parts by mass of the nutrient solution. Spinach was cultivated simultaneously with Example 1, and the content of spinach folic acid on the 15th day after planting was measured. The obtained results are shown in Table 1.

  As shown in Table 1, the spinach of Examples 1 to 3 to which phenylalanine was added had a folic acid content increased about twice as compared with the spinach of Comparative Example 1 to which phenylalanine was not added. Further, in Reference Example 1 in which methionine, which is another amino acid, was added, the effect of increasing folic acid could be confirmed, but it was found that the increased amount of phenylalanine was more effective. As a result, it was demonstrated that the addition of phenylalanine has an effect of efficiently increasing the folic acid content of the plant to be cultivated.

53 Cultivated bed 61 Cultivated bed row 62 Cultivated bed group 73 Child tank 86 Parent tank 90 Flow meter 91 Ball tap

Claims (6)

A method for cultivating leafy vegetables by supplying nutrient solution,
A plant cultivation method, wherein the nutrient solution is added with phenylalanine.   The plant cultivation method according to claim 1, wherein 0.001 to 2 parts by mass of the phenylalanine is added to 100 parts by mass of the nutrient solution. Place a planting panel board with a lot of planting holes on a cultivation bed with a gradient,
Place the seedling root pot on the cultivation bed through the planting hole,
The plant cultivation method of Claim 1 or 2 which supplies the said nutrient solution to the upper surface side of the bottom face of this cultivation bed, and grows leaf vegetables. A plurality of ridges extending in the gradient direction are provided on the upper surface of the cultivation bed,
The ridge is located below the planting hole,
Between the ridges is a groove,
Place the seedling pot on the ridge,
The plant cultivation method according to claim 3, wherein the nutrient solution is allowed to flow through the recess.   The plant cultivation method according to claim 4, wherein a hydrophilic sheet is disposed on the upper surface side of the bottom surface of the cultivation bed.   The plant cultivation method according to any one of claims 1 to 5, wherein the leafy vegetables are Ominae family, Brassicaceae family, Amaryllidaceae family, Apiaceae family, Lamiaceae family, Amaranthaceae family, Asteraceae family, or Akaza family. .

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