CN1330235C - Rice cultivating method for obtaining high protein seeds - Google Patents
Rice cultivating method for obtaining high protein seeds Download PDFInfo
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- CN1330235C CN1330235C CNB2005100490302A CN200510049030A CN1330235C CN 1330235 C CN1330235 C CN 1330235C CN B2005100490302 A CNB2005100490302 A CN B2005100490302A CN 200510049030 A CN200510049030 A CN 200510049030A CN 1330235 C CN1330235 C CN 1330235C
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- milligrams
- rice
- milligram
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- nutrient solution
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 45
- 235000009566 rice Nutrition 0.000 title claims abstract description 45
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 16
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims abstract description 8
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 44
- 235000015097 nutrients Nutrition 0.000 claims abstract description 20
- 150000001860 citric acid derivatives Chemical class 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 5
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical class [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims description 4
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 3
- 239000003501 hydroponics Substances 0.000 abstract 1
- 235000019624 protein content Nutrition 0.000 description 13
- 235000018102 proteins Nutrition 0.000 description 11
- 235000016709 nutrition Nutrition 0.000 description 9
- 235000013339 cereals Nutrition 0.000 description 8
- 230000035764 nutrition Effects 0.000 description 8
- 235000013305 food Nutrition 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 108010068370 Glutens Proteins 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 235000013312 flour Nutrition 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 2
- 108010016634 Seed Storage Proteins Proteins 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000021125 infant nutrition Nutrition 0.000 description 2
- 206010012434 Dermatitis allergic Diseases 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010073032 Grain Proteins Proteins 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 108700005078 Synthetic Genes Proteins 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 201000008937 atopic dermatitis Diseases 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000021329 brown rice Nutrition 0.000 description 1
- -1 citrate compound Chemical class 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 235000008242 dietary patterns Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229940073490 sodium glutamate Drugs 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Fertilizers (AREA)
Abstract
The present invention discloses a rice cultivating method for obtaining high protein seeds. Rice is grown on a cultivation bed of a greenhouse, the cultivation bed is lightproof, and a nutrient solution is supplied respectively according to different prescriptions in a seedling stage of the rice, a tillering and ear shooting stage and a stage after ear shooting. The pH value of the nutrient solution is adjusted in a timing mode, the nutrient solution is changed in the timing mode, temperature and moisture are controlled, and the rice which can obtain the high protein seeds can be cultivated. The present invention greatly improves the protein content of the rice seeds and solves the problem that hydroponics rice is difficult to set seeds.
Description
Technical field
The present invention relates to the paddy rice planting technology, especially, relate to a kind of rice cultivating method that obtains high protein seeds.
Background technology
The nutrition survey of last century end shows: the protein level deficiency in China resident food, in " the nineties China eating patterns reform and development outline " regulation 2000 72g (good protein accounts for 1/3) level also there is a big difference, holding is not that protein deficient problem in infant's food of present China more than 2,000 ten thousand is particularly serious.Since 4～6 months, the contained heat energy of pure breast milk and other nutrient can not satisfy the needs of infants growth and development approximately, must in time give diatery supplement to the baby and replenish.Have data to show, 0～6 month growth and development state of China baby and advanced country's level are very nearly the same, but 6 months later gaps obviously widen.Studies show that the bad main cause of China's infant nutrition is to fail to add diatery supplement on time, or the quality and quantity that adds diatery supplement can not satisfy the infant nutrition needs.Malnutrition can cause that immunologic function is poor, incidence of disease height, in addition cause IQ lower, of short and small stature.Therefore the exploitation of baby's ablactational food and accesary foods is most important.The baby is in the critical period of growing, and food is had special requirement, requires health, easily digestion, heat and nutritional sufficiency on the one hand, requires the ratio of nutrient suitable on the other hand again.Because rice is a cereal the most widely in the world wide, and the quality of rice protein is the best in all kinds of corn gluten proteins, so rice just becomes the primary raw material of all kinds of child nutrition food first-selections.Abroad to the research of ourishing rice flour early, all lay claim to the production history in more than 100 year as ourishing rice flours such as nest, Heng Shi industry giant.And the research of domestic this respect is started late, so far also with regard to time of more than ten years.Existing studies show that, ourishing rice flour is a kind of baby's ablactational food and accesary foods that has a high potential to improving the infant nutrient situation, promoting that the infant growth effect is remarkable.
Rice is best suited for one of nutrient source of infant, but its low protein content (about 8%) has greatly limited the application potential of rice.The technology that improves the protein content of rice has a good application prospect.At present, mainly improve the protein content of rice from breeding and cultivation angle, China selected some protein contents near in addition be higher than 13% high protein variety.Japan has also isolated the synthetic gene of glutelin, and the controlling gene and the site thereof of relevant glutelin content and content of prolamine have been found, thereby further selected the extra high kind that can improve protein utilization rate of glutelin content, the kidney trouble patient has been had low especially kind of the glutelin content of health-care effect and the globulin content especially low kind powerful to allergic dermatitis.The formation of protein also is subjected to the influence of environmental condition except the restriction that is subjected to inherent cause.Appropriateness applied nitrogen and control temperature can increase grain protein content.These researchs at present are under test, also do not form reliable and stable mature technology.In addition, from results reported, these methods are also undesirable to the effect of the protein content of raising rice.
Water culture technology successfully is used for commercially producing of multiple horticultural crop, but does not almost use in the production of cereal, and the correlative study data is also rare.Find that also water planting paddy rice difficulty is solid in the test in addition.The target of present technique promptly is the protein content that increases substantially rice with the water planting means, thereby provides desirable feedstock for the production of infantile nutritious powder.
Summary of the invention
The object of the present invention is to provide a kind of breeding method that obtains the high protein rice grain, the protein content that makes rice grain is greater than 15%, and the difficult solid problem of solution water planting paddy rice.
For realizing purpose of the present invention, adopt following technical scheme: rice cultivation on greenhouse culture bed, culture bed light tight, three phases is supplied with nutrient solution by prescription A, B, C respectively after the seedling stage of paddy rice, tiller-begin heading and heading, regularly regulates nutrient solution pH value to 5.0～5.3.When electrical conductivity of solution drops to 1/2, change nutrient solution.Day, maximum temperature was controlled at 30～35 ℃, and a day minimum temperature is controlled at 25～27 ℃, and relative moisture is controlled in 75～88% scopes.Described prescription A contains 35 milligrams of NH in every liter of solution
4NO
3, 12 milligrams of NaH
2PO
42H
2O, 22 milligrams of K
2SO
4, 27 milligrams of CaCl
2, 100 milligrams of MgSO
47H
2O, 0.5 milligram of MnCl
24H
2O, 0.03 milligram of (NH
4)
6Mo
7O
247H
2O, 0.4 milligram of H
3BO
3, 0.01 milligram of ZnSO
47H
2O, 0.01 milligram of CuSO
45H
2O, 2.5 milligrams of FeCl
36H
2O, 4.0 milligrams of solubility citrate compounds.Described prescription B contains 140 milligrams of NH in every liter of solution
4NO
3, 50 milligrams of NaH
2PO
42H
2O, 90 milligrams of K
2SO
4, 110 milligrams of CaCl
2, 405 milligrams of MgSO
47H
2O, 1.8 milligrams of MnCl
24H
2O, 0.1 milligram of (NH
4)
6Mo
7O
247H
2O, 1.2 milligrams of H
3BO
3, 0.05 milligram of ZnSO
47H
2O, 0.04 milligram of CuSO
45H
2O, 10.0 milligrams of FeCl
36H
2O, 15.0 milligrams of solubility citrate compounds.Described prescription C contains 105 milligrams of NH in every liter of solution
4NO
3, 36 milligrams of NaH
2PO
42H
2O, 66 milligrams of K
2SO
4, 80 milligrams of CaCl
2, 300 milligrams of MgSO
47H
2O, 1.5 milligrams of MnCl
24H
2O, 1.0 milligrams of (NH
4)
6Mo
7O
247H
2O, 1.2 milligrams of H
3BO
3, 0.03 milligram of ZnSO
47H
2O, 0.03 milligram of CuSO
45H
2O, 7.5 milligrams of FeCl
36H
2O, 12.0 milligrams of solubility citrate compounds, 56 milligrams of sodium glutamates.
The present invention with respect to the beneficial effect of prior art is:
1. greatly improve the protein content of rice grain, use the present invention and can obtain the rice grain of protein content, and the protein content of same breed with the rice grain of conventional land for growing field crops production technology acquisition had only about 7-10% up to 15-16%.
2. paddy rice can be normally solid with water planting of the present invention.
Embodiment
In theory, envirment factor has a significant effect to rice protein content, but it is complicated to influence mechanism, and the mechanism of action also imperfectly understands at present.Under field condition, envirment factor is difficult to control.The present invention is by using the greenhouse water culture technology, accurately control nutrition supply, comprise that the different bearing stage fills a prescription with Different Nutrition, regularly keep the skin wet to and set the nutrient solution volume, regularly regulate nutrient solution pH, in time change nutrient solution, and environmental temperature and relative moisture are controlled within the specific limits, selected suitable kind, reached the purpose of cultivating the high protein rice grain.
With paddy rice be planted in the greenhouse culture bed on, culture bed light tight.The process of growth of paddy rice is divided seedling stage, tiller-begin heading and heading back three phases, supply with rice nutrition liquid by the A that fills a prescription shown in the table 1, B, C respectively, the original volume that keeps the skin wet every morning is surveyed an electrical conductivity on an average of every two days and is surveyed a pH value, and with pH regulator to 5.0～5.3.When electrical conductivity of solution drops to originally 1/2 the time, change one time of nutrition liquid.Day, maximum temperature was controlled at 30～35 ℃, and a day minimum temperature is controlled at 25～27 ℃, and relative moisture is controlled in 75～88% scopes.Note the management of damage by disease and insect and plant type, prevent lodging.Gather in the crops seed in good time.To the air-dry back of seed storage.
In the prescription, prescription B is close with general in the world paddy rice prescription shown in the table 1, but Different Nitrogen Concentration has raising, and the rice nutrition organ has sufficient nitrogen accumulation before can guaranteeing to be in the milk with this prescription in the present invention; The nutrient concentration of prescription A roughly is 1/4 of prescription B, can guarantee the normal growth of rice shoot in the present invention with this prescription, and helps control to tiller; Prescription C has increased sodium glutamate newly, and other nutrient concentrations roughly are 3/4 of prescription B, can guarantee that with this prescription the normal grouting of paddy rice is solid in the present invention.
Table 1. nutrient solution prescription
| Nutritive salt | Prescription A concentration (mg/L) | Prescription B concentration (mg/L) | Prescription C concentration (mg/L) |
| NH 4NO 3 | 35 | 140 | 105 |
| NaH 2PO 4·2H 2O | 12 | 50 | 36 |
| K 2SO 4 | 22 | 90 | 66 |
| CaCl 2 | 27 | 110 | 80 |
| MgSO 4·7H 2O | 100 | 405 | 300 |
| MnCl 2·4H 2O | 0.5 | 1.8 | 1.5 |
| (NH 4) 6Mo 7O 24·7H 2O | 0.03 | 0.1 | 1.0 |
| H 3BO 3 | 0.4 | 1.2 | 1.2 |
| ZnSO 4·7H 2O | 0.01 | 0.05 | 0.03 |
| CuSO 4·5H 2O | 0.01 | 0.04 | 0.03 |
| FeCl 3·6H 2O | 2.5 | 10.0 | 7.5 |
| The solubility citrate compound | 4.0 | 15.0 | 12.0 |
| Sodium glutamate | - | - | 56 |
Embodiment
Culture vessel is a 17L black plastic bucket.3 clumps of every container plantations, every clump 2 strain paddy rice.Plant is fixed with foam.Divide seedling stage, supply with nutrient solution by prescription A, B, C respectively after tiller-begin heading and the heading, original volume keeps the skin wet every morning, survey an electrical conductivity on an average of every two days and survey a pH value, and with pH regulator to 5.0, when electrical conductivity of solution drops to originally 1/2 the time, change one time of nutrition liquid.Day, maximum temperature was controlled at 30～35 ℃, and a day lowest temperature is controlled at 25～27 ℃, and relative moisture is controlled in 75～88% scopes.Note the management of damage by disease and insect and plant type, prevent lodging.Gather in the crops seed in good time.To the air-dry back of seed storage.Results brown rice detects with Flash EA1112 CHN elemental analyser, and protein content is 16.3%.
Claims (1)
1. a rice cultivating method that obtains high protein seeds is characterized in that, rice cultivation on greenhouse culture bed is culture bed light tight, supplies with nutrient solution by prescription A, B, C respectively at seedling stage of paddy rice, tiller-begin heading and heading back three phases; Regularly regulate nutrient solution pH value to 5.0～5.3, when electrical conductivity of solution drops to 1/2, change nutrient solution; Day, maximum temperature was controlled at 30～35 ℃, and a day minimum temperature is controlled at 25～27 ℃, and relative moisture is controlled in 75～88% scopes; Wherein, described prescription A contains 35 milligrams of NH in every liter of solution
4NO
3, 12 milligrams of NaH
2PO
42H
2O, 22 milligrams of K
2SO
4, 27 milligrams of CaCl
2, 100 milligrams of MgSO
47H
2O, 0.5 milligram of MnCl
24H
2O, 0.03 milligram of (NH
4)
6Mo
7O
247H
2O, 0.4 milligram of H
3BO
3, 0.01 milligram of ZnSO
47H
2O, 0.01 milligram of CuSO
45H
2O, 2.5 milligrams of FeCl
36H
2O, 4.0 milligrams of solubility citrate compounds; Described prescription B contains 140 milligrams of NH in every liter of solution
4NO
3, 50 milligrams of NaH
2PO
42H
2O, 90 milligrams of K
2SO
4, 110 milligrams of CaCl
2, 405 milligrams of MgSO
47H
2O, 1.8 milligrams of MnCl
24H
2O, 0.1 milligram of (NH
4)
6Mo
7O
247H
2O, 1.2 milligrams of H
3BO
3, 0.05 milligram of ZnSO
47H
2O, 0.04 milligram of CuSO
45H
2O, 10.0 milligrams of FeCl
36H
2O, 15.0 milligrams of solubility citrate compounds; Described prescription C contains 105 milligrams of NH in every liter of solution
4NO
3, 36 milligrams of NaH
2PO
42H
2O, 66 milligrams of K
2SO
4, 80 milligrams of CaCl
2, 300 milligrams of MgSO
47H
2O, 1.5 milligrams of MnCl
24H
2O, 1.0 milligrams of (NH
4)
6Mo
7O
247H
2O, 1.2 milligrams of H
3BO
3, 0.03 milligram of ZnSO
47H
2O, 0.03 milligram of CuSO
45H
2O, 7.5 milligrams of FeCl
36H
2O, 12.0 milligrams of solubility citrate compounds, 56 milligrams of sodium glutamates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100490302A CN1330235C (en) | 2005-02-03 | 2005-02-03 | Rice cultivating method for obtaining high protein seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100490302A CN1330235C (en) | 2005-02-03 | 2005-02-03 | Rice cultivating method for obtaining high protein seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1647611A CN1647611A (en) | 2005-08-03 |
| CN1330235C true CN1330235C (en) | 2007-08-08 |
Family
ID=34876509
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100490302A Expired - Fee Related CN1330235C (en) | 2005-02-03 | 2005-02-03 | Rice cultivating method for obtaining high protein seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1330235C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102875221B (en) * | 2012-09-17 | 2014-04-16 | 四川省农业科学院土壤肥料研究所 | Method for preparing nutrient solution of rice containing stable ferrous ions |
| CN105110864A (en) * | 2015-08-20 | 2015-12-02 | 林平 | Premixed ingredient for rice nutrient solution of International Rice Research Institute |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1429479A (en) * | 2001-12-30 | 2003-07-16 | 福建农林大学 | Breeding method of large embryo rice variety |
| CN1545855A (en) * | 2003-12-03 | 2004-11-17 | 上海市宝山区农业良种繁育场 | Method for seed selection and cultivation of new species rice |
| CN1552874A (en) * | 2003-06-02 | 2004-12-08 | 上海师范大学 | Method for producing rice seed with protein concentration improvement |
-
2005
- 2005-02-03 CN CNB2005100490302A patent/CN1330235C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1429479A (en) * | 2001-12-30 | 2003-07-16 | 福建农林大学 | Breeding method of large embryo rice variety |
| CN1552874A (en) * | 2003-06-02 | 2004-12-08 | 上海师范大学 | Method for producing rice seed with protein concentration improvement |
| CN1545855A (en) * | 2003-12-03 | 2004-11-17 | 上海市宝山区农业良种繁育场 | Method for seed selection and cultivation of new species rice |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1647611A (en) | 2005-08-03 |
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