JP2018027099A5 - - Google Patents
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- JP2018027099A5 JP2018027099A5 JP2017216772A JP2017216772A JP2018027099A5 JP 2018027099 A5 JP2018027099 A5 JP 2018027099A5 JP 2017216772 A JP2017216772 A JP 2017216772A JP 2017216772 A JP2017216772 A JP 2017216772A JP 2018027099 A5 JP2018027099 A5 JP 2018027099A5
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- JP
- Japan
- Prior art keywords
- cells
- pluripotent stem
- human pluripotent
- stem cells
- primitive ectoderm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000001778 pluripotent stem cell Anatomy 0.000 claims 12
- 210000001705 ectoderm cell Anatomy 0.000 claims 11
- 210000004027 cells Anatomy 0.000 claims 6
- 210000002966 Serum Anatomy 0.000 claims 4
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 4
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 4
- 108010023082 activin A Proteins 0.000 claims 4
- 239000003102 growth factor Substances 0.000 claims 4
- 238000000338 in vitro Methods 0.000 claims 2
- 210000001161 Embryo, Mammalian Anatomy 0.000 claims 1
- 210000001671 Embryonic Stem Cells Anatomy 0.000 claims 1
- 210000001900 Endoderm Anatomy 0.000 claims 1
- 102100007408 FGF5 Human genes 0.000 claims 1
- 101700010264 FGF5 Proteins 0.000 claims 1
- 102100014966 FZD10 Human genes 0.000 claims 1
- 101700071936 FZD10 Proteins 0.000 claims 1
- 210000003716 Mesoderm Anatomy 0.000 claims 1
- 210000000472 Morula Anatomy 0.000 claims 1
- 102100019163 POU5F1 Human genes 0.000 claims 1
- 101710026246 POU5F1 Proteins 0.000 claims 1
- 108010023079 activin B Proteins 0.000 claims 1
- 230000004069 differentiation Effects 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 230000001850 reproductive Effects 0.000 claims 1
Claims (16)
- 原始外胚葉細胞をインビトロで産生する方法であって、
（ａ）ヒト多能性幹細胞に、前記ヒト多能性幹細胞が原始外胚葉細胞に分化するのを促進するのに十分な量の少なくとも一つのＴＧＦβスーパーファミリー成長因子を提供する工程であって、前記原始外胚葉細胞は、中胚葉または胚体内胚葉に分化し得る多能性細胞である、工程、
（ｂ）前記原始外胚葉細胞を十分な時間生成させ、それにより原始外胚葉細胞を産生する工程
を含む前記方法。 - 前記ＴＧＦβスーパーファミリー成長因子が、ノーダル、アクチビンＡ又はアクチビンＢである、請求項１記載の方法。
- 前記ＴＧＦβスーパーファミリー成長因子がアクチビンＡである、請求項２記載の方法。
- 前記ヒト多能性幹細胞に、少なくとも１０ｎｇ／ｍｌのアクチビンＡが提供される、請求項３記載の方法。
- 前記ヒト多能性幹細胞に、少なくとも１００ｎｇ／ｍｌのアクチビンＡが提供される、請求項３記載の方法。
- 前記ヒト多能性幹細胞がヒト胚性幹細胞である、請求項１〜５のいずれか１項に記載の方法。
- 前記ヒト多能性幹細胞が、桑実胚、胚の内部細胞塊（ＩＣＭ）又は胚の生殖隆起に由来する、請求項１〜６のいずれか１項に記載の方法。
- 前記ヒト多能性幹細胞に血清を提供する工程をさらに含む、請求項１〜７のいずれか１項に記載の方法。
- 前記ヒト多能性幹細胞に、２％（ｖ／ｖ）未満の血清を含む培地を提供する工程をさらに含む、請求項１〜８のいずれか１項に記載の方法。
- 前記原始外胚葉細胞が、ＦＺＤ１０、ＦＧＦ５及びＯＣＴ４から選ばれる少なくとも一つのマーカーを発現する、請求項１〜９のいずれか１項に記載の方法。
- 前記ヒト多能性幹細胞の少なくとも１５％が前記原始外胚葉細胞に分化する、請求項１〜１０のいずれか１項に記載の方法。
- 前記ヒト多能性幹細胞の少なくとも５０％が前記原始外胚葉細胞に分化する、請求項１〜１０のいずれか１項に記載の方法。
- 前記ヒト多能性幹細胞の少なくとも９０％が前記原始外胚葉細胞に
分化する、請求項１〜１０のいずれか１項に記載の方法。 - 原始外胚葉細胞をインビトロで産生する方法であって、
ヒト多能性幹細胞を含む細胞集団に、ＴＧＦβスーパーファミリー成長因子を提供し、少なくとも１０％の原始外胚葉細胞を含む細胞集団を産生する工程を含む前記方法。 - 前記細胞集団を、血清を含まない培地で培養する、請求項１４記載の方法。
- 前記細胞集団を、血清代替物を含まない培地で培養する、請求項１４記載の方法。
Applications Claiming Priority (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US58656604P | 2004-07-09 | 2004-07-09 | |
| US60/586,566 | 2004-07-09 | ||
| US58794204P | 2004-07-14 | 2004-07-14 | |
| US60/587,942 | 2004-07-14 | ||
| US11/021,618 | 2004-12-23 | ||
| US11/021,618 US7510876B2 (en) | 2003-12-23 | 2004-12-23 | Definitive endoderm |
| US69336405P | 2005-06-23 | 2005-06-23 | |
| US60/693,364 | 2005-06-23 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016076972A Division JP6473708B2 (ja) | 2004-07-09 | 2016-04-07 | 前原始線条及び中内胚葉細胞 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020068885A Division JP7161665B2 (ja) | 2004-07-09 | 2020-04-07 | 前原始線条及び中内胚葉細胞 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018027099A JP2018027099A (ja) | 2018-02-22 |
| JP2018027099A5 true JP2018027099A5 (ja) | 2018-04-05 |
| JP6777620B2 JP6777620B2 (ja) | 2020-10-28 |
Family
ID=40262947
Family Applications (7)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007520513A Active JP5433879B2 (ja) | 2004-07-09 | 2005-07-08 | 前原始線条及び中内胚葉細胞 |
| JP2013150092A Active JP5956388B2 (ja) | 2004-07-09 | 2013-07-19 | 前原始線条及び中内胚葉細胞 |
| JP2015086989A Active JP6077044B2 (ja) | 2004-07-09 | 2015-04-21 | 前原始線条及び中内胚葉細胞 |
| JP2016076972A Active JP6473708B2 (ja) | 2004-07-09 | 2016-04-07 | 前原始線条及び中内胚葉細胞 |
| JP2017216772A Active JP6777620B2 (ja) | 2004-07-09 | 2017-11-09 | 前原始線条及び中内胚葉細胞 |
| JP2020068885A Active JP7161665B2 (ja) | 2004-07-09 | 2020-04-07 | 前原始線条及び中内胚葉細胞 |
| JP2022113855A Active JP7525102B2 (ja) | 2004-07-09 | 2022-07-15 | 前原始線条及び中内胚葉細胞 |
Family Applications Before (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007520513A Active JP5433879B2 (ja) | 2004-07-09 | 2005-07-08 | 前原始線条及び中内胚葉細胞 |
| JP2013150092A Active JP5956388B2 (ja) | 2004-07-09 | 2013-07-19 | 前原始線条及び中内胚葉細胞 |
| JP2015086989A Active JP6077044B2 (ja) | 2004-07-09 | 2015-04-21 | 前原始線条及び中内胚葉細胞 |
| JP2016076972A Active JP6473708B2 (ja) | 2004-07-09 | 2016-04-07 | 前原始線条及び中内胚葉細胞 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020068885A Active JP7161665B2 (ja) | 2004-07-09 | 2020-04-07 | 前原始線条及び中内胚葉細胞 |
| JP2022113855A Active JP7525102B2 (ja) | 2004-07-09 | 2022-07-15 | 前原始線条及び中内胚葉細胞 |
Country Status (10)
| Country | Link |
|---|---|
| EP (2) | EP1773986B1 (ja) |
| JP (7) | JP5433879B2 (ja) |
| CN (1) | CN102586170B (ja) |
| AU (1) | AU2005271944B2 (ja) |
| CA (1) | CA2573354C (ja) |
| DK (2) | DK1773986T3 (ja) |
| ES (2) | ES2754038T3 (ja) |
| MX (1) | MX2007000317A (ja) |
| NZ (1) | NZ582597A (ja) |
| WO (1) | WO2006017134A2 (ja) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20050266554A1 (en) | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| US7541185B2 (en) | 2003-12-23 | 2009-06-02 | Cythera, Inc. | Methods for identifying factors for differentiating definitive endoderm |
| US7985585B2 (en) | 2004-07-09 | 2011-07-26 | Viacyte, Inc. | Preprimitive streak and mesendoderm cells |
| WO2007059007A2 (en) | 2005-11-14 | 2007-05-24 | Cythera, Inc. | Markers of definitive endoderm |
| US8647873B2 (en) | 2004-04-27 | 2014-02-11 | Viacyte, Inc. | PDX1 expressing endoderm |
| US7510876B2 (en) | 2003-12-23 | 2009-03-31 | Cythera, Inc. | Definitive endoderm |
| US7625753B2 (en) | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
| JP5102030B2 (ja) * | 2004-08-13 | 2012-12-19 | ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド | ヒト胚性幹細胞における自己再生および分化のための組成物および方法 |
| AU2005322007B2 (en) * | 2004-12-23 | 2011-06-30 | Viacyte, Inc. | Expansion of definitive endoderm cells |
| FI20055398A0 (fi) | 2005-07-08 | 2005-07-08 | Suomen Punainen Risti Veripalv | Menetelmä solupopulaatioiden evaluoimiseksi |
| DK1957636T3 (en) | 2005-10-27 | 2018-10-01 | Viacyte Inc | PDX1-EXPRESSING DORSAL AND VENTRAL FORTARM ENDODERM |
| SG10201405107YA (en) | 2006-02-23 | 2014-10-30 | Viacyte Inc | Compositions and methods useful for culturing differentiable cells |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| US11254916B2 (en) | 2006-03-02 | 2022-02-22 | Viacyte, Inc. | Methods of making and using PDX1-positive pancreatic endoderm cells |
| EP1999253B1 (en) | 2006-03-02 | 2019-05-22 | Viacyte, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
| WO2008013664A2 (en) | 2006-07-26 | 2008-01-31 | Cythera, Inc. | Methods of producing pancreatic hormones |
| WO2008048647A1 (en) * | 2006-10-17 | 2008-04-24 | Cythera, Inc. | Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells |
| US7695963B2 (en) | 2007-09-24 | 2010-04-13 | Cythera, Inc. | Methods for increasing definitive endoderm production |
| US8008075B2 (en) | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
| US8895300B2 (en) | 2008-11-04 | 2014-11-25 | Viacyte, Inc. | Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof |
| EP2356227B1 (en) | 2008-11-14 | 2018-03-28 | Viacyte, Inc. | Encapsulation of pancreatic cells derived from human pluripotent stem cells |
| RU2576003C2 (ru) | 2008-12-03 | 2016-02-27 | Интернэшнл Стем Селл Корпорейшн | Способ получения дифференцированных клеток из стволовых клеток |
| CN114173837A (zh) | 2019-05-31 | 2022-03-11 | W.L.戈尔及同仁股份有限公司 | 生物相容性膜复合材料 |
| CN114206407A (zh) | 2019-05-31 | 2022-03-18 | W.L.戈尔及同仁股份有限公司 | 生物相容性膜复合材料 |
| EP3976236A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates Inc. | A biocompatible membrane composite |
| JP7328362B2 (ja) | 2019-05-31 | 2023-08-16 | ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド | 制御された酸素拡散距離を伴う細胞カプセル化デバイス |
| KR20230051519A (ko) | 2020-08-15 | 2023-04-18 | 리제너론 파마슈티칼스 인코포레이티드 | 칼시토닌 수용체 (calcr)를 인코딩하는 변이체 핵산 분자를 갖는 대상체에서 비만의 치료 |
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| EP2361966A1 (en) * | 2002-05-17 | 2011-08-31 | Mount Sinai School of Medicine of New York University | Mesoderm and definitive endoderm cell populations |
| US20040229350A1 (en) | 2003-05-12 | 2004-11-18 | Nikolai Strelchenko | Morula derived embryonic stem cells |
| US7510876B2 (en) * | 2003-12-23 | 2009-03-31 | Cythera, Inc. | Definitive endoderm |
| JP5102030B2 (ja) * | 2004-08-13 | 2012-12-19 | ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド | ヒト胚性幹細胞における自己再生および分化のための組成物および方法 |
| US11586805B2 (en) | 2021-07-26 | 2023-02-21 | Atlassian Pty Ltd. | Machine-learning-based natural language processing techniques for low-latency document summarization |
-
2005
- 2005-07-08 JP JP2007520513A patent/JP5433879B2/ja active Active
- 2005-07-08 ES ES10013206T patent/ES2754038T3/es active Active
- 2005-07-08 CA CA2573354A patent/CA2573354C/en active Active
- 2005-07-08 DK DK05769491.1T patent/DK1773986T3/en active
- 2005-07-08 CN CN201210020574.6A patent/CN102586170B/zh active Active
- 2005-07-08 ES ES05769491T patent/ES2716827T3/es active Active
- 2005-07-08 NZ NZ582597A patent/NZ582597A/en not_active IP Right Cessation
- 2005-07-08 MX MX2007000317A patent/MX2007000317A/es active IP Right Grant
- 2005-07-08 DK DK10013206T patent/DK2341147T3/da active
- 2005-07-08 WO PCT/US2005/024161 patent/WO2006017134A2/en active Application Filing
- 2005-07-08 EP EP05769491.1A patent/EP1773986B1/en active Active
- 2005-07-08 EP EP19151982.6A patent/EP3505622A1/en active Pending
- 2005-07-08 AU AU2005271944A patent/AU2005271944B2/en active Active
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2013
- 2013-07-19 JP JP2013150092A patent/JP5956388B2/ja active Active
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2015
- 2015-04-21 JP JP2015086989A patent/JP6077044B2/ja active Active
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2016
- 2016-04-07 JP JP2016076972A patent/JP6473708B2/ja active Active
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2017
- 2017-11-09 JP JP2017216772A patent/JP6777620B2/ja active Active
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2020
- 2020-04-07 JP JP2020068885A patent/JP7161665B2/ja active Active
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2022
- 2022-07-15 JP JP2022113855A patent/JP7525102B2/ja active Active
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