JP2017119633A - Agent for promoting growth and/or suppressing decrease in bifidobacterium bacteria and/or lactic acid bacteria - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide technologies for promoting the growth of bifidobacteria or lactic acid bacteria in an environment where these bacteria can grow or maintain the cell number, and for suppressing the cell decrease in an environment where their growth or survival is difficult.SOLUTION: The invention provides an agent comprising Peucedanum japonicum as an effective ingredient for promoting the growth of Bifidobacterium bacteria and/or lactic acid bacteria and/or for suppressing their decrease. Also provided is an oral composition comprising Peucedanum japonicum and at least one strain of bacteria selected from the group consisting of Bifidobacterium bacteria and lactic acid bacteria. Also provided is a method for promoting the growth of Bifidobacterium bacteria and/or lactic acid bacteria and/or for suppressing their decrease, which method comprises a step in which at least one strain of bacteria selected from the group consisting of Bifidobacterium bacteria and lactic acid bacteria and Peucedanum japonicum coexist.SELECTED DRAWING: None

Description

  The present technology relates to a growth promoting and / or reducing inhibitor used for promoting or suppressing the growth of Bifidobacterium (hereinafter also simply referred to as “Bifidobacterium”) and / or lactic acid bacteria, and the growth promoting and The present invention relates to a method for suppressing decrease. The present technology also relates to an oral composition containing bifidobacteria and / or lactic acid bacteria together with the growth promoting and / or decrease inhibitor.

  Bifidobacteria are resident bacteria in the gut of mammals (including humans) and are not pathogenic in their own right. Rather, they antagonize pathogenic enterobacteria in terms of lactic acid and acetic acid production and auxotrophy, It is known to have an action of inhibiting the growth of these bacteria in the intestinal tract.

  In general, bifidobacteria are dominant in the intestinal flora (intestinal flora) in the large intestine of infants, but the flora varies with aging. Specifically, bifidobacteria decrease from adolescence to middle age, and spoilage bacteria such as Clostridium bacteria and Escherichia coli increase markedly. As a result, the intestinal environment deteriorates and adversely affects the health of the host (person). For this reason, in order to maintain health for a long time as a person ages, it is extremely important to keep the intestinal flora in a state in which useful bacteria such as bifidobacteria are always dominant.

  On the other hand, lactic acid bacteria are usually contained in dairy products such as yogurt and fermented foods such as pickles, and when they are eaten by mammals (including humans), they enter the intestine and normal balance of enteric bacteria is normal. Or exert an intestinal regulating action. Recently, lactic acid bacteria have the ability to suppress allergic diseases such as hay fever, atopic dermatitis, asthma, prevent influenza infection by regulating immune mechanisms, and reduce good cholesterol in the blood. It has also been shown that an effect of suppressing and lowering the value of neutral fat can be expected.

  For these reasons, substances that promote the growth of bifidobacteria and lactic acid bacteria (hereinafter also simply referred to as “growth promoters”) have been sought, and many growth promoters have been developed and proposed.

  Currently known as Bifidobacteria growth promoters include, for example, oligosaccharides such as fructooligosaccharide, galactooligosaccharide, xylo-oligosaccharide, isomaltoligosaccharide, and soybean oligosaccharide; N-acetylglucosamine, lactulose, raffinose, cinderose, Sugars such as cyclodextrin and konjac mannan (Patent Documents 1 to 4, Non-Patent Documents 1 to 3); Soymilk and Soymilk Extract (Patent Documents 5 to 6); Tea Extract (Patent Document 7); Calcium Phosphate (Patent Document 8); Bovine lactoferrin, bovine apolactophyrin, bovine lactophilin iron (Patent Document 9) and the like can be mentioned.

JP-A-60-41449 Japanese Patent Laid-Open No. 3-183454 JP-A-57-138385 JP-A-10-175867 Japanese Patent Publication No. 45-9822 JP 59-179064 A Japanese Patent Laid-Open No. 1-191680 JP 2005-130804 A JP-A-8-38044

"Bifidobacterium", p. 77, 1979, Yakult Co., Ltd. "Chemistry and Biology", 21, 291, 1983, Academic Publishing Center "RIKEN Intestinal Flora Symposium, Intestinal Flora and Nutrition", p. 89, 1983, Academic Publishing Center

As described above, there has been a demand for further development of substances that promote the growth of bifidobacteria and lactic acid bacteria. Bifidobacteria and lactic acid bacteria may be reduced if their growth is promoted by the environment in which they exist, or if the number of bacteria remains unchanged.
Therefore, the present technology provides a technology that can promote proliferation in an environment where bifidobacteria and lactic acid bacteria grow or have no change in the number of bacteria, and can suppress a decrease in an environment that is difficult to survive or grow (grow). The main purpose.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have promoted the proliferation significantly by coexisting button-bow fu, which is a perennial plant of the Apiaceae family, with bifidobacteria and / or lactic acid bacteria. In other words, it has been found that button bowfish has an effect of promoting the growth of bifidobacteria and / or lactic acid bacteria.

That is, the present technology first provides an agent for promoting and / or reducing the growth of Bifidobacterium bacteria and / or lactic acid bacteria, which comprises button fountain as an active ingredient.
The proliferation promoting and / or decrease suppressing agent according to the present technology may further contain at least one selected from the group consisting of proteins and carbohydrates.
In this case, milk protein can be selected as the protein.
Moreover, as said saccharide | sugar, at least 1 sort (s) selected from the group which consists of a monosaccharide, a disaccharide, and an oligosaccharide can be selected.

Next, with this technology,
An oral composition comprising at least one bacterium selected from the group consisting of Bifidobacterium and lactic acid bacteria is provided.
The oral composition according to the present technology may further contain at least one selected from the group consisting of proteins and carbohydrates.
In this case, milk protein can be selected as the protein.
Moreover, as said saccharide | sugar, at least 1 sort (s) selected from the group which consists of a monosaccharide, a disaccharide, and an oligosaccharide can be selected.
Furthermore, the oral composition can be used for improving gut microbiota, immune regulation, diarrhea, constipation, obesity, or prevention and / or treatment of inflammatory bowel disease.

In the present technology, a Bifidobacterium bacterium and / or a lactic acid bacterium further comprising a step of coexisting at least one bacterium selected from the group consisting of a Bifidobacterium bacterium and a lactic acid bacterium and a button bud. A method for promoting proliferation and / or suppressing decrease is provided.
In the method for promoting growth and / or suppressing decrease according to the present technology, at least one selected from the group consisting of proteins and carbohydrates can be allowed to coexist.
In this case, milk protein can be selected as the protein.
Moreover, as said saccharide | sugar, at least 1 sort (s) selected from the group which consists of a monosaccharide, a disaccharide, and an oligosaccharide can be selected.

  According to the present technology, it is possible to promote proliferation in an environment in which bifidobacteria and lactic acid bacteria grow or have no change in the number of bacteria, and to suppress a decrease in an environment in which it is difficult to survive or grow (grow). In addition, the effect described here is not necessarily limited, and may be any effect described in the present technology.

  Hereinafter, preferred embodiments for carrying out the present technology will be described. In addition, embodiment described below shows an example of typical embodiment of this indication, and, thereby, the range of this technique is not interpreted narrowly. In the present specification, regarding what expresses a numerical range as “lower limit to upper limit”, the upper limit may be “below” or “less than”, and the lower limit may be “more than”. It may be “super”.

1. Growth-promoting and / or decrease inhibitor The growth-promoting and / or decrease-inhibiting agent according to the present technology (hereinafter, also simply referred to as “proliferation-promoting / decrease-inhibiting agent”) is a preparation containing button bowfish as an active ingredient, Effect and / or suppression of promoting the growth of at least one bacterium selected from the group consisting of bacteria and lactic acid bacteria (hereinafter simply referred to as “Bifidobacteria and / or lactic acid bacteria” or “Bifidobacteria / lactic acid bacteria”) It has the effect to do. Moreover, the growth promotion and / or decrease inhibitor of the present technology may contain at least one selected from the group consisting of proteins and carbohydrates, in addition to buttonfish.
Hereinafter, the present technology will be described in detail.

(1) Bifidobacterium Bifidobacterium targeted by the present technology includes, for example, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium infantis (Bifidobacterium infantis) ), Bifidobacterium adolescentis, Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium animalis, etc. . These bifidobacteria may be used alone or in any combination of two or more. Although not limited, Bifidobacterium longum, Bifidobacterium breve, and Bifidobacterium infantis are preferable. Bifidobacterium longum and Bifidobacterium breve are more preferable.

(2) Lactic acid bacteria Lactococcus lactis subsp. Cremoris (Lactococcus lactis subsp. Cremoris) (Lactococcus lactis subsp. Cremoris) Streptococcus salivarius subsp. Thermophilus (hereinafter also referred to as Streptococcus bacterium, Enterococcus bacterium); Leuconostoc mesenteroides subsp. cremoris (Leuconostock bacteria); Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus delbu Bulgaricus (Lactobacillus delbrueckii subsp. Bulgaricus), Lactobacillus delbrueckii subsp. Lactis (Lactobacillus delbrueckii subsp. Lactis), Lactobacillus gasseri (Lactobacillus gasseri), Lactobacillus helcilactus L Talam (Lactobacillus plantarum), Lactobacillus brevis (Lactobacillus brevis), Lactobacillus casei subspices rhamnosus (Lactobacillus casei subsp. Rhamnosus), Lactobacillus paracasei (Lactobacillus paracasei) (Tetragenococcus halophilus) (above, Tetragenococcus spp.) And Pediococcus pentosaseus (above, Pegococcus pentosaseus) Iokokkasu bacteria belonging to the genus), and the like. These lactic acid bacteria may be used alone or in any combination of two or more. Although not limited, Lactobacillus gasseri, Lactobacillus acidophilus, and Lactobacillus paracasei are preferable. More preferred is Lactobacillus paracasei.

(3) Button Bow The button bow (scientific name: Peucedanum japonicum Thunb. Var. Japonicum), which is the subject of this technology, is a perennial plant belonging to the genus Papaveraceae. Also known as long life grass, it is a plant distributed mainly in the west of central Honshu, Shikoku, Kyushu, Okinawa, southern Korea, China, and the Philippines.

  The growth promoting / decrease suppressing agent of the present technology may be composed of 100% by weight of button fountain, and may contain other components in addition to button fountain as described above. For this reason, the content ratio of button bow fu in the growth promoting / decreasing inhibitor of the present technology can be appropriately adjusted in the range of 0.01 to 100% by mass, preferably 0.03 to 100% by mass, more preferably. It can be 0.3-100 mass%, Most preferably, it can be 3-100 mass%.

  In addition, the growth promoting / decreasing agent of the present technology is such that the final concentration of button fountain is 0.0001 to 100% by mass, depending on the final form of a pharmaceutical or quasi-drug or food and drink described below It can be used in a proportion such that it is preferably 0.03 to 100 mass%, particularly preferably 0.3 to 100 mass%.

  In addition, when taking the growth promoting / decreasing agent of the present technology orally for the purpose of growing and / or suppressing the decrease of bifidobacteria and / or lactic acid bacteria in the living intestine, the amount taken per day is In terms of the amount of button fountain contained in the growth-promoting / decrease-inhibiting agent of the present technology, 10 mg to 20 g / day, preferably 30 mg to 10 g / day, more preferably 1 to 6 g / day can be mentioned. . The intake may be divided into two or more times a day.

(4) Protein As described above, the growth promoting / decreasing inhibitor of the present technology can be blended with protein in addition to buttonfish. By blending the protein, the effect of promoting the growth and / or suppressing the decrease of Bifidobacterium and / or lactic acid bacteria is further improved, as shown in the Examples described later.

As a protein that can be used in the growth promoting / decrease suppressing agent of the present technology, one or more proteins that can be used in the fields of medicine and food can be freely selected and used. Specifically, for example, proteins derived from milk such as casein and whey protein, and proteins derived from plants such as soybean protein can be mentioned.
Examples of casein that can be used include various commercially available caseins and caseinates. More specifically, lactate casein, sulfate casein, casein hydrochloride, sodium caseinate, potassium caseinate, calcium caseinate, magnesium caseinate, or any mixture thereof can be used. Moreover, casein etc. refine | purified by the conventional method from cow's milk, skim milk, whole fat milk powder, and skim milk powder can also be utilized.
As whey protein, whey (eg, whey powder, desalted whey powder, etc.) separated from commercially available products or from milk, skim milk, etc. by a known method, or separated and purified whey protein concentrate, whey protein isolated Or mixtures of these in any proportions.
These proteins can be used in a purified form as a protein, but can also be used in the form of a raw material containing these proteins.

  Raw materials containing milk-derived proteins include raw milk, milk, buffalo milk, goat milk, sheep milk, horse milk, concentrated milk, skim concentrated milk, skim milk powder, whey protein concentrate (WPC), and whey protein isolate. (WPI), milk protein concentrate (MPC), micellar casein isolate (MCI), milk protein isolate (MPI) and the like.

  “Milk” in this technology includes raw milk, milk, special milk, and raw goat milk stipulated in the “Ministry of Health, Labor and Welfare Ordinance on Component Standards for Milk and Dairy Products” (hereinafter simply referred to as “Ministerial Ordinance for Milk”) , Pasteurized goat milk, partially skimmed milk, skimmed milk and processed milk (mammalian breast milk). These may be used alone or in combination with the growth promoter of the present technology, or two or more thereof may be combined arbitrarily. Preferred are breast milk and soy milk of the above mammals, more preferably raw milk derived from cattle, cow milk, special cow milk, partially skimmed milk, skimmed milk and processed milk; and soy milk. The definition of “raw milk, milk, special milk, raw goat milk, pasteurized goat milk, partially skimmed milk, skimmed milk and processed milk” is as defined by a ministerial ordinance such as milk.

  By the way, the average composition of milk is 3.3% protein, 3.8% lipid, 4.8% carbohydrate, minerals (potassium, calcium, phosphate, magnesium, sodium, citrate, phosphorus, iron) Etc.) 0.37% by weight, vitamins (vitamins A, B1, B2, C and E), and water, about 67K calories per 100 grams. About 80% by mass of the protein contained in milk is casein (casein micelle). The remaining 20% by mass is whey protein, which includes proteins such as β-lactoglobulin, α-lactoglobulin, immunoglobulin, serum albumin, and lactoferrin. Examples of the carbohydrate contained in milk include lactose (disaccharide), glucose, galactose (above, monosaccharide), and other oligosaccharides. Most carbohydrates are lactose and occupy 50 mass% of solid content of skim milk powder. Most of the remaining 50% by mass of skim milk powder (solid content) is protein.

Examples of raw materials containing plant-derived proteins include soy milk, almond milk, and coconut milk. The soy milk, almond milk, coconut milk and the like used in the present invention may be defined as “plant-derived milk”.
Soy milk is made by soaking soybeans in water and adding the water to the soup.
Almond milk is made by crushing almonds soaked in water with a mixer, adding water, and rinsing them with gauze.
Coconut milk is a sweet milky ingredient obtained from solid endosperm that is layered inside mature coconut seeds.

Moreover, the product (for example, dairy product etc.) which processed the raw material containing protein further can also be mix | blended with the proliferation promotion / decrease inhibitor of this technique.
The dairy product means an edible product obtained by artificially processing the aforementioned milk (preferably raw milk and soy milk). For example, dairy products prepared from raw milk include cream, butter, butter oil, cheese, concentrated whey, ice cream (ice cream, lacto ice, ice milk), concentrated milk, defatted concentrated milk, sugar-free milk, sugar-free Skim milk, sweetened milk, sweetened skim milk, whole milk powder, skim milk powder, cream powder, whey powder, buttermilk powder, sweetened powdered milk, adjusted powdered milk, fermented milk, lactic acid bacteria beverage (non-fat milk solid content 3% or more And dairy drinks). The definition of each of these components shall be based on the ordinance of milk. Among these, non-fat milk (whey, concentrated whey, cottage cheese, non-fat concentrated milk, sugar-free skim milk, sweetened skim milk, non-fat concentrated milk, sugar-free skim milk, sweetened skim milk, non-fat dry milk, whey powder ), Powdered milk (whole powdered milk, skim milk powder, cream powder, whey powder, buttermilk powder, sweetened powdered milk, adjusted powdered milk), and more preferably skim milk powder and whey powder.

  When protein is blended with the growth promoting / decreasing inhibitor of the present technology, the blending amount is not particularly limited, and can be freely set according to the amount of button fountain and the purpose of use. In particular, in the present technology, it is preferable to add 1 to 10000 parts by mass, and more preferably 50 to 300 parts by mass with respect to 100 parts by mass of the button bow.

(5) Carbohydrate As described above, the growth promoting / decreasing inhibitor of the present technology may contain a sugar in addition to the button bow. By adding a saccharide, the effect of promoting the growth and / or suppressing the decrease of Bifidobacterium and / or lactic acid bacteria is further improved, as shown in the Examples described later.

  As the carbohydrate that can be used in the growth promoting / decreasing agent of the present technology, one or two or more carbohydrates that can be used in the fields of medicine, food, etc., can be freely selected and used. it can. Specifically, for example, monosaccharides such as glucose, fructose, galactose, rhamnose and fucose; disaccharides such as lactose, sucrose, maltose and trehalose; trisaccharides such as raffinose; lactulose, fructooligosaccharides, galactooligosaccharides, Examples thereof include oligosaccharides such as mannan oligosaccharide, isomaltoligosaccharide and xylooligosaccharide; polysaccharides such as N-acetylglucosamine, dextrin and soybean oligosaccharide. Preferred are monosaccharides, disaccharides and oligosaccharides, and more preferred are glucose and lactose. Lactulose is obtained by alkaline isomerization of lactose by a known method, and can be obtained commercially, but can also be produced according to the method described in Japanese Patent Publication No. Sho 52-21063.

  When a saccharide is blended in the growth promoting / decreasing inhibitor of the present technology, the blending amount is not particularly limited, and can be freely set according to the amount of button fountain and the purpose of use. In this technique, it is preferable to mix | blend 1-20000 mass parts especially with respect to 100 mass parts of button bow fu, and it is more preferable to mix | blend 80-500 mass parts.

(6) Other components The growth promoting / decreasing inhibitor of this technology is free of one or more other components that can be used in the fields of medicine, food, etc., as long as the effects of this technology are not impaired. Can be selected and used.

  Other ingredients can include carriers and additives used to formulate button buds. Specifically, for example, components such as an excipient, a pH adjuster, a colorant, and a corrigent can be used. Moreover, it is also possible to use together the component which has the effect of prevention, improvement, and / or treatment of the well-known disease or symptom discovered in the future according to the objective suitably.

  As the preparation carrier, various organic or inorganic carriers can be used depending on the dosage form. Examples of the carrier in the case of a solid preparation include excipients, binders, disintegrants, lubricants, stabilizers, and flavoring agents.

  Examples of the excipient include sugar derivatives such as lactose, sucrose, glucose, mannitol and sorbit; starch derivatives such as corn starch, potato starch, α-starch, dextrin and carboxymethyl starch; crystalline cellulose and hydroxypropylcellulose Cellulose derivatives such as hydroxypropylmethylcellulose, carboxymethylcellulose, carboxymethylcellulose calcium; gum arabic; dextran; pullulan; silicate derivatives such as light anhydrous silicic acid, synthetic aluminum silicate, magnesium magnesium magnesium silicate; phosphate derivatives such as calcium phosphate; Examples thereof include carbonate derivatives such as calcium carbonate; sulfate derivatives such as calcium sulfate.

  Examples of the binder include gelatin, polyvinyl pyrrolidone, macrogol and the like in addition to the excipients described above.

  Examples of the disintegrant include, in addition to the above excipients, chemically modified starch or cellulose derivatives such as croscarmellose sodium, carboxymethyl starch sodium, and crosslinked polyvinylpyrrolidone.

  Examples of the lubricant include talc; stearic acid; stearic acid metal salts such as calcium stearate and magnesium stearate; colloidal silica; waxes such as pea gum and gallow; boric acid; glycol; carboxyl such as fumaric acid and adipic acid Acids; sodium carboxylic acid salts such as sodium benzoate; sulfates such as sodium sulfate; leucine; lauryl sulfates such as sodium lauryl sulfate and magnesium lauryl sulfate; silicic acids such as anhydrous silicic acid and silicic acid hydrate; starch derivatives, etc. Can be mentioned.

  Examples of the stabilizer include paraoxybenzoates such as methyl paraben and propyl paraben; alcohols such as chlorobutanol, benzyl alcohol and phenylethyl alcohol; benzalkonium chloride; acetic anhydride; sorbic acid and the like.

Examples of the flavoring agent include sweeteners, acidulants, and fragrances.
In addition, as a carrier used in the case of a liquid for oral administration, a solvent such as water, a flavoring agent and the like can be mentioned.

(7) Use The growth promoting / decreasing inhibitor of the present technology exclusively promotes the growth of bifidobacteria / lactic acid bacteria, suppresses the decrease, increases the number of viable bacteria, or survives the bacteria. Used to improve performance.

In the present technology, “proliferation promotion” (growth promoting action) means acting on bifidobacteria / lactic acid bacteria (live bacteria) in a viable environment to promote the growth.
In the present technology, “decrease suppression” (decrease suppression action) means that by promoting the growth of the bifidobacteria / lactic acid bacteria in an environment where it is difficult to survive or grow (proliferate), It means that the decrease in the number of bacteria can be suppressed and the number of cells remaining in the living state (viable cell count) can be maintained or increased (improvement of survival rate).

These growth promotion and reduction inhibitory effects of the growth promotion / reduction inhibitor of this technology are the same as before and after the culture when bifidobacteria / lactic acid bacteria (viable bacteria) are cultured in the presence of the growth promotion / reduction inhibitor of this technology. The survival rate (%) calculated from the ratio of the number of viable Bifidobacteria / Lactic acid bacteria in the following formula is calculated in the absence of the growth promoting / decreasing inhibitor of this technology. ) Can be judged as an index compared to the survival rate (%) (control survival rate) calculated when cultivating. In this specification, “culture” means that the bifidobacteria / lactic acid bacteria can grow in the presence of only the growth-promoting / decrease-inhibiting agent of this technology without the nutrients necessary for the growth of bifidobacteria / lactic acid bacteria. The state maintained at various temperatures is also included.
The specific culture method and conditions of bifidobacteria / lactic acid bacteria can refer to the methods described in the experimental examples described later.

[Equation 1]
Survival rate (%) = [number of viable cells after cultivation / number of viable cells before cultivation] × 100

  In calculating the survival rate (%), the number of viable bacteria of bifidobacteria / lactic acid bacteria can be determined based on a standard method. Examples of the method for measuring the number of viable bacteria include a colony counting method using a TOS propionic acid agar medium (Yakult Pharmaceutical Co., Ltd.) as a method for measuring bifidobacteria. An example of a method for measuring lactic acid bacteria is a colony counting method using a BCP-added plate count agar medium “Eiken” (Eiken Chemical Co., Ltd.).

  The growth promoting / decreasing agent of the present technology promotes the growth of bifidobacteria / lactic acid bacteria or suppresses the decrease, thereby improving intestinal flora, immune regulation, diarrhea, constipation, obesity, or inflammatory bowel Since it is useful for prevention and / or treatment of diseases and the like, it can be used in the form of pharmaceuticals or quasi-drugs, foods and drinks, and feeds based on these uses.

(7-1) Drugs and Quasi-drugs Growth-promoting / decrease-inhibiting agents according to the present technology are used for human or veterinary drugs, pharmaceuticals by utilizing the excellent bifidobacteria / lactic acid-bacteria growth-promoting / decreasing effect. It can be blended and used as an active ingredient such as quasi-drug.

When blended with a pharmaceutical product, the pharmaceutical product can be appropriately formulated into a desired dosage form according to an administration method such as oral administration or parenteral administration. The dosage form is not particularly limited, but for oral administration, for example, solid preparations such as powders, granules, tablets, troches and capsules; liquid preparations such as solutions, syrups, suspensions and emulsions. Can be In the case of parenteral administration, it can be formulated into, for example, suppositories, sprays, inhalants, ointments, patches, injections and the like. In the present disclosure, it is preferable to formulate into a dosage form for oral administration.
The formulation can be appropriately performed by a known method depending on the dosage form.

(7-2) Food / beverage products The growth promoting / decreasing inhibitor according to the present technology uses the excellent bifidobacteria / lactic acid bacteria growth promoting / decreasing effect, and is a health food for humans or animals, a functional food, It can be used in combination with these as active ingredients such as foods for the sick, enteral nutritional foods, special purpose foods, functional health foods, foods for specified health use, functional indication foods, and functional nutritional foods.

  The proliferation promoting / decreasing inhibitor according to the present technology can be prepared by adding to a known food or drink, or can be mixed with a raw material of a food or drink to produce a new food or drink.

  The food and drink may be in the form of liquid, paste, solid, powder, etc., in addition to tablet confectionery, liquid food, etc., for example, flour products, instant foods, processed agricultural products, processed fishery products, processed livestock products, milk -Dairy products, fats and oils, basic seasonings, compound seasonings / foods, frozen foods, confectionery, beverages, commercial products other than these.

Examples of the flour product include bread, macaroni, spaghetti, noodles, cake mix, fried flour, bread crumbs and the like.
Examples of the instant foods include instant noodles, cup noodles, retort / cooked food, cooking canned food, microwave food, instant soup / stew, instant miso soup / soup, canned soup, freeze-dried food, other instant foods, etc. Can be mentioned.
Examples of the processed agricultural products include canned agricultural products, canned fruits, jams and marmalades, pickles, boiled beans, dried agricultural products, and cereals (cereal processed products).
Examples of the processed fishery products include canned fishery products, fish hams and sausages, fishery paste products, fishery delicacies, and tsukudani.
Examples of the processed livestock products include canned livestock products / pastes, livestock meat hams / sausages, and the like.
Examples of the milk / dairy products include processed milk, milk beverages, yogurts, lactic acid bacteria beverages, cheese, ice creams, prepared milk powders, creams, and other dairy products.
Examples of the fats and oils include butter, margarines, and vegetable oils.
Examples of the basic seasonings include soy sauce, miso, sauces, tomato processed seasonings, mirins, vinegars, etc., and the mixed seasonings and foods include cooking mixes, curry ingredients, and sauces , Dressings, noodle soups, spices, and other complex seasonings.
As said frozen food, raw material frozen food, semi-cooked frozen food, cooked frozen food, etc. are mentioned, for example.
Examples of the confectionery include caramel, candy, chewing gum, chocolate, cookies, biscuits, cakes, pie, snacks, crackers, Japanese confectionery, rice confectionery, bean confectionery, dessert confectionery, and other confectionery.
Examples of the beverage include carbonated beverages, natural fruit juices, fruit juice beverages, soft drinks with fruit juice, fruit beverages, fruit beverages with fruit granules, vegetable beverages, soy milk, soy milk beverages, coffee beverages, tea beverages, powdered beverages, and concentrated beverages. Sports drinks, nutritional drinks, alcoholic drinks, other taste drinks, and the like.
Examples of commercially available foods other than the above include baby foods, sprinkles, and tea paste.

In addition, the food and drink defined in the present disclosure can be provided and sold as a food and drink displaying specific uses (particularly health uses) and functions.
The “display” act includes all acts for informing the consumer of the use, and if the expression can remind the user of the use, the purpose of the display, the content of the display, the display Regardless of the target object / medium, etc., all fall under the “display” act of this disclosure.

  Moreover, it is preferable that the “display” is performed by an expression that allows the consumer to directly recognize the above-described use. Specifically, it is the act of transferring, displaying, importing, displaying, or importing products that are related to food or drinks or products that describe the use, on advertisements, price lists, or transaction documents. For example, an act of describing and displaying the above uses or distributing them, or describing the above uses in information including the contents and providing them by an electromagnetic (Internet or the like) method can be given.

  On the other hand, the display content is preferably a display approved by the government or the like (for example, a display that is approved based on various systems determined by the government and performed in a mode based on such approval). Moreover, it is preferable to attach such display contents to advertising materials at sales sites such as packaging, containers, catalogs, pamphlets, POPs, and other documents.

  “Indications” include health foods, functional foods, foods for the sick, enteral nutritional foods, special purpose foods, health functional foods, foods for specified health use, functional labeling foods, nutritional functional foods, and pharmaceutical departments. The display as a foreign article etc. is also mentioned. Among these, in particular, indications approved by the Consumer Affairs Agency, for example, indications approved by food systems for specific health use, functional indication food systems, systems similar to these, and the like. More specifically, indication as food for specified health use, indication as conditional health food, indication as functional indication food, indication to affect body structure and function, indication of reduced disease risk, etc. Can be mentioned. A typical example of this is the indication of food for specified health use (particularly indication of health use) as stipulated in the Enforcement Regulations of the Health Promotion Act (Ministry of Health, Labor and Welfare Ordinance No. 86 of April 30, 2003) This is a display as a functional indication food defined by the Food Labeling Act (Act No. 70 of 2013) and a similar indication.

(7-3) Feed The growth promoting / decreasing inhibitor according to the present technology can be used as an active ingredient of animal feed, utilizing the excellent growth promoting / decreasing inhibitory effect of bifidobacteria / lactic acid bacteria. . The growth promoting / decreasing inhibitor according to the present technology can be prepared by adding to a known feed, or can be mixed in the feed raw material to produce a new feed.

  Examples of the raw material of the feed include cereals such as corn, wheat, barley, and rye; bran such as bran, wheat straw, rice bran, and defatted rice bran; and manufactured porridges such as corn gluten meal and corn jam meal; Animal feeds such as whey, fish meal and bone meal; yeasts such as beer yeast; mineral feeds such as calcium phosphate and calcium carbonate; fats and oils; amino acids; In addition, examples of the form of the feed include pet animal feed (pet food, etc.), livestock feed, fish feed, and the like.

2. Oral composition The oral composition which concerns on this technique is a composition which contains at least button pea and at least 1 sort (s) of bacteria selected from the group which consists of Bifidobacterium genus bacteria and lactic acid bacteria. Moreover, at least 1 sort (s) selected from the group which consists of protein and carbohydrate can be contained as needed. The oral composition can be used for the prevention and / or treatment of intestinal microbiota improvement, immune regulation, diarrhea, constipation, obesity, inflammatory bowel disease and the like.

The content of bifidobacteria / lactic acid bacteria in the oral composition is not particularly limited, and can be freely set according to the purpose of use. In the present technology, in particular, bifidobacteria and / or lactic acid bacteria (total amount when both are included) in 1 g of the oral composition are usually 1 × 10 ⁴ cfu / g or more, preferably 1 × 10 ⁶ to 1 × 10 ¹² cfu. / G.

  The details of the bifidobacteria / lactic acid bacterium, button fountain, protein, saccharide, and other components are the same as those of the growth promoting and / or reducing inhibitor described above, and thus the description thereof is omitted here.

3. Bifidobacteria / Lactic Acid Bacteria Growth Promotion and / or Reduction Suppression Method The Bifidobacterium / lactic acid bacteria growth promotion and / or reduction suppression method of the present technology (hereinafter, also simply referred to as “growth promotion / reduction suppression method”) is Bifidobacterium. It is a method having a step of coexisting at least one kind of bacteria selected from the group consisting of genus bacteria and lactic acid bacteria and button fountain. Moreover, at least 1 sort (s) selected from the group which consists of protein and carbohydrate can also coexist as needed.

  The details of the bifidobacteria / lactic acid bacterium, button fountain, protein, saccharide, and other components are the same as those of the above-described growth promoting and / or decreasing inhibitor and oral composition, and thus description thereof is omitted here.

  Hereinafter, the present technology will be described in more detail based on examples. In addition, the Example described below shows an example of a typical example of the present technology, and the scope of the present technology is not interpreted narrowly.

<Experimental example 1>
In Experimental Example 1, the effect of button bow fu on the growth of Bifidobacteria was examined.
In addition, Bifidobacterium longum (Bifidobacterium longum) was used as a Bifidobacterium. More specifically, Bifidobacterium longum has been deposited with the American Type Culture Collection (ATCC) under the deposit number “BAA-999” (Bifidobacterium longum ATCC BAA-999) and is commercially available. (Http://www.atcc.org/products/all/BAA-999.aspx) was used (hereinafter the same).
As the button bow powder, “Yonaguni Chomeizo Aojiru” manufactured by Kinshu Bio Co., Ltd. was used (hereinafter the same).

(1) Preparation of each experimental material (1-1) Preparation of bifidobacteria After inoculating various bifidobacteria in a medium containing protein, amino acid, and sugar source and culturing at 32-41 ° C. for 5-24 hours The cells (wet cells) were collected from the culture solution by centrifugation. Lyophilization was performed for 18 to 96 hours using a freeze dryer (manufactured by Kyowa Vacuum Co., Ltd.), and the cell mass after completion of freeze drying was physically pulverized to obtain various bifidobacteria.

(1-2) Preparation of button fountain solution 3 g of button fountain powder was dissolved in 100 mL of tap water or milk to prepare a button fountain solution, which was sterilized by autoclaving at 90 ° C. for 10 minutes.

(2) Experimental method The number of bacteria per 10 mL becomes 1.0 × 10 ⁹ cfu (1.0 × 10 ⁸ cfu / mL) in each 10 mL amount of each button bow solution (sterilized) put in a test tube. Were inoculated with various Bifidobacterium diluted with physiological saline and cultured at 37 ° C. for 8 hours and 16 hours. After culturing, the number of viable bacteria (cfu / mL) and pH in the culture are measured, and compared with the number of viable bacteria and pH before culturing, the button fountain for the growth and survival (survival rate) of bifidobacteria The impact of. For comparison, the same experiment was performed using tap water or milk instead of the button-bowl solution.

(3) Experimental results Table 1 shows the results of Examples 1-2 and Comparative Examples 1-2.

As can be seen from the comparison between Example 1 and Comparative Example 1, by culturing bifidobacteria in the presence of button fountain, a decrease in the viable count of bifidobacteria is suppressed, and as a result, the survival rate increases. It has been found.
In addition, as shown in Comparative Example 2, although milk has an effect of increasing the survival rate of bifidobacteria, as shown in Example 2, the growth of bifidobacteria is enhanced by combining button fountain with milk. It was further promoted that the number of viable bacteria increased.

<Experimental example 2>
In Experimental Example 2, the effect of button swell on the growth of lactic acid bacteria was examined.
In addition, Lactobacillus gasseri (Lactobacillus gasseri) and Lactobacillus paracasei (Lactobacillus paracasei) were used as lactic acid bacteria. More specifically, as Lactobacillus gasseri, a strain that has been deposited with the Patent Microorganism Depositary, National Institute of Technology and Evaluation, under the deposit number “NITE BP-01669”, can be obtained commercially. As the Lactobacillus paracasei, bacteria that have been deposited at the Patent Microorganism Depositary, National Institute for Product Evaluation and Technology under the deposit number “NITE BP-01633”, and are commercially available were used.

(1) Preparation of each experimental material (1-1) Preparation of lactic acid bacteria Lactic acid bacteria are inoculated into a medium containing protein, amino acid, and sugar source, and cultured at 32 to 41 ° C for 5 to 24 hours. The cells (wet cells) were collected by centrifugation. Freeze-drying was performed for 18 to 96 hours using a freeze-dryer (manufactured by Kyowa Vacuum Co., Ltd.), and the cell mass after completion of freeze-drying was physically pulverized to obtain lactic acid bacteria.

(1-2) Preparation of button fountain solution 3 g of button fountain powder was dissolved in 100 mL of tap water or milk to prepare a button fountain solution, which was sterilized by autoclaving at 90 ° C. for 10 minutes.

(2) Experimental method The number of bacteria per 10 mL becomes 1.0 × 10 ⁹ cfu (1.0 × 10 ⁸ cfu / mL) in each 10 mL amount of each button bow solution (sterilized) put in a test tube. Was inoculated with lactic acid bacteria diluted with physiological saline and cultured at 37 ° C. for 8 hours. After culturing, the number of viable bacteria in the culture (cfu / mL) and pH are measured, compared with the number of viable bacteria and pH before culturing, and the growth rate of lactic acid bacteria and the survival rate (survival rate). The impact was evaluated. For comparison, the same experiment was performed using tap water or milk instead of the button-bowl solution.

(3) Experimental results Table 2 shows the results of Examples 3 to 6 and Comparative Examples 3 to 6.

As can be seen from the comparison between Example 3 and Comparative Example 3 and the comparison between Example 4 and Comparative Example 4, lactic acid bacteria can also be cultured by culturing in the presence of button fountain, as in Experimental Example 1. It was found that the growth was promoted and the survival was significantly increased.
In addition, as shown in Comparative Examples 4 and 6, although milk has an effect of increasing the survival of lactic acid bacteria, as shown in Examples 4 and 6, by combining button fountain with milk, bifidobacteria It was found that the growth of the bacteria was further promoted and the number of viable bacteria was significantly increased.

<Experimental example 3>
In Experimental Example 3, the effect of combined use of button pea and protein and / or carbohydrate on the growth of Bifidobacteria was examined.
In addition, Bifidobacterium longum (Bifidobacterium longum) was used as a Bifidobacterium.

(1) Preparation of each experimental material (1-1) Preparation of bifidobacteria Bifidobacteria were prepared in the same manner as in experimental example 1.

(1-2) Preparation of button-up solution [Examples 7 and 8]
3 g of button bow powder and protein (casein or whey protein) were dissolved in 100 mL of tap water to prepare a button bow solution having a protein concentration of 3% by mass. This button-bowl solution was sterilized by autoclaving at 90 ° C. for 10 minutes.

[Example 9]
3 g of button bow powder and sugar (lactose) were dissolved in 100 mL of tap water to prepare a button bow solution having a sugar concentration of 5% by mass. This button-bowl solution was sterilized by autoclaving at 90 ° C. for 10 minutes.

(2) Experimental method The number of bacteria per 10 mL becomes 1.0 × 10 ⁹ cfu (1.0 × 10 ⁸ cfu / mL) in each 10 mL amount of each button bow solution (sterilized) put in a test tube. Were inoculated with Bifidobacterium diluted with physiological saline and cultured at 37 ° C. for 8 hours and 16 hours. After culturing, the number of viable bacteria (cfu / mL) and pH in the culture are measured, and compared with the number of viable bacteria and pH before culturing, the button fountain for the growth and survival (survival rate) of bifidobacteria The effects of proteins and carbohydrates were evaluated. For comparison, instead of the above button-bowl solution, an aqueous solution (Comparative Examples 7 and 8) having a protein concentration of 3% by mass in which protein (casein or whey protein) is dissolved in tap water and sugar (lactose) are respectively supplied in tap water. Experiments were similarly performed using an aqueous solution (Comparative Example 9) having a sugar concentration of 5 mass% dissolved in water.

(3) Experimental results Table 3 shows the results of Examples 7 to 9 and Comparative Examples 7 to 9.

  As shown in Comparative Examples 7 and 8, when bifidobacteria are cultured in the presence of protein alone, the viability of the bacteria is reduced, whereas as shown in Examples 7 and 8, button fountains are combined. Thus, it was found that the growth of bifidobacteria was promoted and the number of viable bacteria increased. In addition, as can be seen from the comparison between Example 9 and Comparative Example 9, it was found that the survival of bifidobacteria also increased with carbohydrates in combination with button bowfish.

<Experimental example 4>
In Experimental Example 4, the effect of button swell concentration on the growth and survival of bifidobacteria was examined.
In addition, Bifidobacterium longum (Bifidobacterium longum) was used as a Bifidobacterium.

(1) Preparation of each experimental material (1-1) Preparation of bifidobacteria Bifidobacteria were prepared in the same manner as in experimental example 1.

(1-2) Preparation of button fountain solution Each button fountain solution was prepared by dissolving button fountain powder in 100 mL of milk so as to have the concentrations shown in Table 4 below. This button-bowl solution was sterilized by autoclaving at 90 ° C. for 10 minutes.

(2) Experimental method The number of bacteria per 10 mL becomes 1.0 × 10 ⁹ cfu (1.0 × 10 ⁸ cfu / mL) in each 10 mL amount of each button bow solution (sterilized) put in a test tube. Were inoculated with Bifidobacterium diluted with physiological saline and cultured at 37 ° C. for 8 hours and 16 hours. After culturing, the number of viable bacteria (cfu / mL) and pH in the culture are measured, and compared with the number of viable bacteria and pH before culturing, the button fountain for the growth and survival (survival rate) of bifidobacteria The effect of concentration was evaluated.

(3) Experimental results Table 4 shows the results of Examples 10 to 14.

  As shown in Table 4, in each Example, it was confirmed that the pH after culturing for 8 hours was lower than the pH before culturing, and the decrease in the number of viable bacteria was suppressed. It is apparent that the effect of suppressing the decrease in the number of viable bacteria is dependent on the concentration of button fountain. When the amount of button fountain is 3% by mass or more, the number of viable bacteria at the start of culture is maintained even after 8 hours. It was. In Example 14 where the concentration of button fountain was 10% by mass, it was found that the effect turned from the suppression of the decrease in the number of viable bacteria to the effect of promoting growth, and the growth of bifidobacteria was remarkably promoted.

<Experimental example 5>
In Experimental Example 5, the influence of various green juice products on the growth and survival (survival rate) of bifidobacteria was examined.
In addition, Bifidobacterium longum (Bifidobacterium longum) was used as a Bifidobacterium.
Moreover, the following products were used as various green juice products.
Product A / content ingredients: barley young leaves, sugar, dietary fiber, etc. Product B / content ingredients: barley young leaves, seaweed, dextrin, chlorella, etc. Product C / content ingredients: sugar, dextrin, barley young leaves, green tea powder, etc. Product D / content ingredients : Barley young leaves, sugars, etc. Product E / Content ingredients: Barley young leaves Product F / Content ingredients: Barley young leaves, kale, collagen, dextrin, sugars, etc. Product G / Content ingredients: Chlorella

(1) Preparation of each experimental material (1-1) Preparation of bifidobacteria powder Bifidobacteria powder was prepared in the same manner as in Experimental Example 1.

(1-2) Preparation of green juice Various green juice products were prepared by dissolving 3 g of various green juice products in 100 mL of tap water, and sterilized by autoclaving at 90 ° C. for 10 minutes.

(2) Experimental method The number of bacteria per 10 mL is 1.0 × 10 ⁹ cfu (1.0 × 10 ⁸ cfu / mL) in various types of green juice (sterilized) in 10 mL. Bifidobacteria diluted with physiological saline was inoculated and cultured at 37 ° C. for 8 hours. After culturing, the viable cell count (cfu / mL) and pH in the culture were measured, and compared with the viable cell count and pH before culturing, the influence of various green juice products on the growth of bifidobacteria was evaluated. In addition, it replaced with said various green juices for the comparison, and it experimented similarly using a tap water.

(3) Experimental results Table 5 shows the results of Comparative Examples A to G. For reference, the results of Example 1 are also shown.

  As shown in Table 5, it was found that the growth promotion effect of bifidobacteria was greatly different in various green juice products as compared with Example 1 containing button bow fu. Moreover, in most of the green juice products, a decrease in the viable count of bifidobacteria was confirmed, and the result that the survivability decreased as compared with Example 1 was confirmed. From such a result, it became clear that among green juice products, the present invention including button swell exhibits the effect of significantly improving the survival rate of bifidobacteria.

Claims (13)

  An agent for promoting and / or reducing the growth of Bifidobacterium and / or lactic acid bacteria, comprising button bow fu as an active ingredient.   Furthermore, the proliferation promotion and / or reduction inhibitor of Claim 1 containing at least 1 sort (s) selected from the group which consists of protein and carbohydrate.   The growth promotion and / or reduction inhibitor according to claim 2, wherein the protein is milk protein.   The growth promoting and / or reducing inhibitor according to claim 2 or 3, wherein the carbohydrate is at least one selected from the group consisting of monosaccharides, disaccharides and oligosaccharides. Button bow,
An oral composition comprising at least one bacterium selected from the group consisting of Bifidobacterium and lactic acid bacteria.   The oral composition according to claim 5, further comprising at least one selected from the group consisting of proteins and carbohydrates.   The oral composition according to claim 6, wherein the protein is milk protein.   The oral composition according to claim 6 or 7, wherein the carbohydrate is at least one selected from the group consisting of monosaccharides, disaccharides and oligosaccharides.   The oral composition as described in any one of Claims 5-8 used for the improvement of an intestinal microflora, immunoregulation, diarrhea, constipation, obesity, or the prevention and / or treatment of an inflammatory bowel disease.   Promotion and / or reduction of growth of Bifidobacterium and / or lactic acid bacteria, comprising the step of coexisting at least one kind of bacteria selected from the group consisting of Bifidobacterium and lactic acid bacteria with button buds Suppression method.   The method according to claim 10, further comprising at least one selected from the group consisting of proteins and carbohydrates.   The method according to claim 11, wherein the protein is milk protein.   The method according to claim 11 or 12, wherein the carbohydrate is at least one selected from the group consisting of monosaccharides, disaccharides and oligosaccharides.