JP2017056981A - Freezing storage container, freezing storage container kit, base material for freezing storage container, and method of manufacturing freezing storage container - Google Patents

Abstract

PROBLEM TO BE SOLVED: To prevent separation of a label stuck on a freezing storage container, enable the use of the freezing storage container in a field requiring a higher-level cleanliness factor than ever, and make its manufacturing cost lower than ever.SOLUTION: A label 10 having a base material layer 11 and an adhesive layer 12 is stuck on a freezing storage container 1. The freezing storage container 1 has a label sticking area 1a where the label 10 is stuck on at least a part of an outer surface 1f. In the label sticking area 1a, arithmetic average roughness Ra is equal to or falls below the thickness t of the adhesive layer 12 and is equal to or exceeds 0.04 times the thickness t of the adhesive layer 12, and the ratio of a surface area to a unit plane area is 5 or more.SELECTED DRAWING: Figure 2

Description

  The present invention relates to a cryopreservation container for freezing and storing contents, a cryopreservation container kit, a base material for a cryopreservation container, and a method for producing a cryopreservation container.

  Conventionally, for example, plastic that adhesive labels affixed to the outer surface of plastic containers such as blood bags and infusion bags are not easily removed due to the effects of water caused by cryogenic temperature or condensation during cryopreservation Containers are known (see Patent Document 1 below).

  The plastic bag for cryopreservation described in Patent Document 1 is formed by sticking an adhesive label on a fiber sheet thermally welded to the outer surface of the cryopreservation plastic. The fiber sheet is made of plastic fiber or paper using a thermoplastic resin compatible with the material of the cryopreservation plastic bag. The fiber sheet made of paper is obtained by coating one or both sides with a thermoplastic resin that is compatible with the material of the cryopreservation plastic bag.

  In Patent Document 1, since the fiber sheet is chemically bonded to the plastic container, it cannot be easily peeled off in moisture or liquid nitrogen. When the adhesive label is attached to the fiber sheet, the adhesive layer of the adhesive label becomes the fiber sheet. It is described that it can be stuck to the surface of a plastic container without being feared of being easily peeled off in moisture or liquid nitrogen.

Japanese Patent No. 3301074

  The cryopreservation container as described above can be used as a container for stably storing cells for a long period of time, for example, in the field of cell medicine such as regenerative medicine, cell-containing pharmaceuticals, and infertility treatment. However, the conventional plastic bag for cryopreservation needs to thermally weld the fiber sheet to the outer surface of the container. Such a fiber sheet may be a source of dust generation and may not be used in the medical field where high cleanliness is required. In addition, the manufacturing cost may increase due to the material cost and the complexity of the manufacturing process.

  The present invention has been made in view of the above problems, can prevent the peeling of the label, can be used in a field that requires a higher degree of cleanliness than before, and has a lower manufacturing cost than before. It is an object of the present invention to provide a cryopreservation container, a cryopreservation container kit, a cryopreservation container substrate, and a method for producing a cryopreservation container that can be reduced.

  In order to achieve the above object, the cryopreservation container of the present invention is a cryopreservation container to which a label having a base material layer and an adhesive layer is attached, and the label is attached to at least a part of the outer surface. The label affixing region has an arithmetic average roughness Ra not more than the thickness of the adhesive layer and not less than 0.04 times the thickness of the adhesive layer, and a ratio of the surface area to the unit flat area is 5 It is the above.

  The cryopreservation container of the present invention may be filled with the contents to be cryopreserved and sealed.

  In the cryopreservation container of the present invention, the label may be attached to the label application region.

  In the cryopreservation container of the present invention, it is preferable that the thickness of the adhesive layer of the label is 10 μm or more when the label is attached to the label attaching region.

  The cryopreservation container kit of the present invention is a cryopreservation container kit comprising a label having a base material layer and an adhesive layer, and a cryopreservation container to which the label is attached, the cryopreservation container having an outer surface A label affixing area where the label is affixed to at least a part of the label, and the label affixing area has an arithmetic average roughness Ra equal to or less than the thickness of the adhesive layer and 0.04 times the thickness of the adhesive layer The ratio of the surface area to the unit flat area is 5 or more.

  The substrate for a cryopreservation container of the present invention is a substrate for a cryopreservation container to which a label having a base material layer and an adhesive layer is attached, and the label is attached to at least a part of the outer surface The label affixing region has an arithmetic average roughness Ra not more than the thickness of the adhesive layer and not less than 0.04 times the thickness of the adhesive layer, and a ratio of the surface area to the unit flat area is 5 It is the above.

  The method for producing a cryopreservation container according to the present invention is a method for producing a cryopreservation container to which a label having a base material layer and an adhesive layer is attached, and at least part of the mold has a thickness equal to or less than the thickness of the adhesive layer. A mold processing step for forming a concavo-convex region by attaching particles having an average particle diameter of the above, and transferring the surface shape of the concavo-convex region of the mold onto at least a part of the outer surface of the cryopreservation container, An uneven transfer process for forming a label application region to which a label is applied, and the label application region has an arithmetic mean roughness Ra equal to or less than the thickness of the adhesive layer and 0.04 of the thickness of the adhesive layer. The ratio of the surface area to the unit flat area is 5 or more.

  According to the cryopreservation container, cryopreservation container kit, cryopreservation container base material, and cryopreservation container manufacturing method of the present invention, it is possible to prevent peeling of a label affixed to a label affixing region, which is more sophisticated than before. It can be used in a field where a high degree of cleanliness is required, and the manufacturing cost can be reduced as compared with the prior art.

The top view of the cryopreservation container which concerns on embodiment of this invention. FIG. 2 is an enlarged cross-sectional view of a label attaching region and a label of the cryopreservation container shown in FIG. 1. The expanded sectional view which shows the state which stuck the label shown in FIG. 2 on the label sticking area | region. Process drawing which shows the manufacturing method of the cryopreservation container shown in FIG.

  Hereinafter, embodiments of a cryopreservation container, a cryopreservation container kit, a cryopreservation container substrate, and a method for producing a cryopreservation container according to the present invention will be described in detail with reference to the drawings. Hereinafter, embodiments of the cryopreservation container will be described first, then embodiments of the cryopreservation container kit and the cryopreservation container substrate will be described, and finally, an embodiment of a method for manufacturing the cryopreservation container will be described.

(Cryopreservation container)
FIG. 1 is a plan view of a cryopreservation container 1 according to this embodiment. The cryopreservation container 1 of this embodiment can be used as a container for stably cryopreserving cells and the like for a long period of time in the field of cellular medicine such as regenerative medicine, cell-containing pharmaceuticals, and infertility treatment. The cryopreservation container 1 is cooled with liquid nitrogen or the like at a temperature of 0 ° C. or lower, more specifically, about −150 ° C. to −196 ° C. in a state in which the content of cells or the like is accommodated. Save the data as it is.

  The cryopreservation container 1 includes a label attaching region 1a for attaching a label, an accommodating portion 1b that accommodates contents such as cells, and the accommodating portion 1b is filled with the contents, and the contents are sent from the accommodating portion 1b. A tube 1c and a port 1d. For example, the tube 1c is fused after the contents are filled in the housing portion 1b, so that the end portion is welded and sealed. The port 1d has an on-off valve 1e, and is opened or sealed by operating the on-off valve 1e.

  In FIG. 1, a bag-shaped cryopreservation container 1 in which a storage portion 1b is formed between flexible sheets and a sheet around the storage portion 1b is closed is illustrated. It is not limited to a shape, Arbitrary shapes, such as a cylinder shape, can be employ | adopted. Further, the cryopreservation container 1 illustrated in FIG. 1 has a label pasting region 1a at a position that does not overlap the accommodating portion 1b of the outer surface 1f, but the position of the label pasting region 1a is not particularly limited, and is cryopreserved. The entire outer surface 1f of the container 1 may be used as the label attaching region 1a.

  2 is an enlarged cross-sectional view of the label attaching region 1a and the label 10 of the cryopreservation container 1 shown in FIG. The label 10 attached to the label attaching region 1 a of the cryopreservation container 1 includes a base material layer 11 and an adhesive layer 12. A main material constituting the base material layer 11 of the label 10 is, for example, a resin material such as white polyester, and a main material constituting the adhesive layer 12 is, for example, an acrylic pressure-sensitive adhesive.

  On the surface of the base material layer 11 of the label 10, identification information or the like of the contents filled in the storage portion of the cryopreservation container 1 is printed. An adhesive layer 12 for attaching the label 10 to the label application region 1 a of the cryopreservation container 1 is provided on the back surface of the base material layer 11 of the label 10. The label 10 has a thickness of about 30 μm to 100 μm, for example, the base material layer 11 has a thickness of about 20 μm to 90 μm, for example, and the adhesive layer 12 has a thickness of about 10 μm to 80 μm, for example. ing.

  The label sticking region 1a of the cryopreservation container 1 has minute irregularities formed on the surface, and the arithmetic average roughness Ra is equal to or less than the thickness t of the adhesive layer 12 of the label 10 and 0 of the thickness t of the adhesive layer 12 of the label 10. 0.04 times or more, and the ratio of the surface area to the unit plane area is 5 or more. The ratio of the surface area to the arithmetic average roughness Ra and the unit flat area of the label attaching region 1a can be calculated based on the surface shape measured using a laser microscope, for example.

  More specifically, in order to calculate the arithmetic average roughness Ra of the label attaching region 1a, for example, using a laser microscope, the label attaching region 1a includes a plurality of minute convex portions 1g and concave portions 1h in a predetermined measurement range. Measure the three-dimensional shape. The predetermined measurement range of the label sticking area 1a can be, for example, a 200 μm square range at an arbitrary position of the label sticking area 1a. The arithmetic average roughness Ra can be measured according to, for example, JIS B 0601-1994.

  Then, based on the measured three-dimensional shape, a roughness curve at a predetermined reference length along the reference line is obtained to calculate the arithmetic average roughness Ra. For example, it is possible to set the reference length to 200 μm, calculate the arithmetic average roughness Ra along reference lines in three directions, and set the average value as the arithmetic average roughness Ra of the label pasting area 1a. Here, a portion protruding upward from the average line of the roughness curve can be defined as a convex portion 1g, and a portion recessed below the average line can be defined as a concave portion 1h.

  Further, in order to calculate the ratio of the surface area to the unit flat area of the label pasting area 1a, the three-dimensional shape of the label pasting area 1a is measured in a measurement area having a predetermined flat area, for example, using a laser microscope. The plane area of the region for measuring the three-dimensional shape can be defined as a unit plane area. Furthermore, based on the measured three-dimensional shape, the surface area of the three-dimensional shape including a plurality of minute convex portions 1g and concave portions 1h is calculated. Then, by using the calculated surface area of the three-dimensional shape and the flat area of the measurement region of the three-dimensional shape, that is, the unit flat area, the ratio of the surface area to the unit flat area of the label pasting region 1a can be calculated. . That is, the ratio of the surface area to the unit flat area of the label application region 1a can be obtained by (surface area of the label application region 1a in the measurement region) / (plane area of the measurement region). If the unevenness of the label attaching region 1a is small, this ratio becomes close to 1, and this ratio increases as the unevenness of the label attaching region 1a increases.

  Hereinafter, the operation of the cryopreservation container 1 of the present embodiment will be described. FIG. 3 is an enlarged cross-sectional view showing a state where the label 10 shown in FIG. 2 is attached to the label attaching region 1a.

  Since the label sticking region 1a of the cryopreservation container 1 has an arithmetic average roughness Ra of 0.04 times or more the thickness t of the adhesive layer 12 of the label 10, a plurality of microscopic items formed in the label sticking region 1a. The convex part 1g and the concave part 1h have an average height of 0.04 times or more the thickness t of the adhesive layer 12. Moreover, since the ratio of the surface area to the unit flat area of the label attaching region 1a is 5 or more, the plurality of minute protrusions 1g and recesses 1h having the above average height are changed to the ratio of the surface area to the unit flat area. It has a corresponding predetermined density.

  Therefore, when the adhesive layer 12 is adhered to the label application region 1a and the label 10 is applied, a plurality of minute protrusions 1g of the label application region 1a bite into the adhesive layer 12, and the adhesive layer 12 includes a plurality of label application regions 1a. Enter the minute recess 1h. As a result, the adhesive layer 12 can be firmly adhered to the label attaching region 1a of the cryopreservation container 1, and the contents of the cryopreservation container 1 are changed due to temperature change during freezing or thawing, and due to moisture such as condensation. The peeling of the label 10 can be prevented.

  Moreover, since the label sticking area | region 1a has arithmetic mean roughness Ra below the thickness t of the adhesion layer 12 of the label 10, it is the some small convex part 1g and recessed part which were formed in the label sticking area | region 1a. 1 h has an average height equal to or less than the thickness t of the adhesive layer 12. That is, most of the minute protrusions 1 g formed in the label pasting area 1 a do not penetrate the adhesive layer 12 of the label 10 and bite into the adhesive layer 12 without reaching the base material layer 11 of the label 10.

  Moreover, since there are few convex parts 1g of the label sticking area | region 1a which penetrates the adhesion layer 12 of the label 10, it bites into the base material layer 11, for example, is deform | transformed by the base material layer 11. FIG. Thereby, it can prevent that the base material layer 11 of the label 10 bends by the convex part 1g of the label sticking area | region 1a which reached the base material layer 11 of the label 10, and the adhesive layer 12 of the label 10 and label sticking The adhesiveness between the region 1a and the adhesive force of the label 10 can be improved.

  In addition, it is preferable that the ratio of the surface area to the arithmetic average roughness Ra and the unit flat area of the label attaching region 1a is measured in two or more measuring ranges that do not overlap in the label attaching region 1a, and each measuring range is preferably separated by 1 mm or more. In each measurement range, the arithmetic average roughness Ra of the labeling region 1a is not more than the thickness t of the adhesive layer 12 and not less than 0.04 times the thickness t of the adhesive layer 12, and the ratio of the surface area to the unit flat area Is 5 or more. Thereby, peeling of the label 10 stuck to the label sticking area | region 1a of the cryopreservation container 1 can be prevented more reliably.

  The cryopreservation container 1 is hermetically sealed by prefilling the container 1b with the contents to be cryopreserved via the tube 1c or the port 1d, and fusing the tube 1c or closing the on-off valve 1e of the port 1d. It may be. This eliminates the need for a user who uses the cryopreservation container 1 or its contents to fill the cryopreservation container 1 with the contents, thereby preventing contamination and the like when filling the contents.

  As for the cryopreservation container 1, the label 10 may be affixed previously to the label affixing region 1a. This eliminates the need for the user who uses the cryopreservation container 1 or its contents to affix the label 10 to the cryopreservation container 1, saves the user trouble, and eliminates the label affixing error by the user. Can do.

  In this case, if the thickness t of the pressure-sensitive adhesive layer 12 of the label 10 is 10 μm or more, not only can the adhesive strength of the pressure-sensitive adhesive layer 12 of the label 10 be sufficiently secured, but also the minute protrusion 1g of the label application region 1a. And the height of the recessed part 1h can be 0.4 micrometer or more. Therefore, the minute convex part 1g of the label sticking area 1a can surely bite into the adhesive layer 12 of the label 10, and the adhesive layer 12 of the label 10 can surely get stuck into the minute concave part 1h of the label sticking area 1a. The adhesive layer 12 of the label 10 can be firmly adhered to the label pasting region 1a.

  The thickness t of the adhesive layer 12 of the label 10 is more preferably 20 μm or more. Thereby, not only the adhesive force by the adhesion layer 12 of the label 10 can be further strengthened, but also the heights of the minute convex portions 1g and the concave portions 1h in the label attaching region 1a can be 0.8 μm or more. Therefore, the minute convex part 1g of the label sticking area 1a can be surely bited by the adhesive layer 12 of the label 10, and the adhesive layer 12 of the label 10 can be surely penetrated by the minute concave part 1h of the label sticking area 1a. The adhesive layer 12 of the label 10 can be firmly adhered to the label pasting region 1a.

  Furthermore, since the cryopreservation container 1 of the present embodiment does not include fibers that may become a dust generation source in the label attaching region 1a, it can be used in fields where higher cleanliness is required than before. . Further, since it is not necessary to use a fiber sheet as in the prior art, the material cost can be reduced, the manufacturing process can be facilitated, and the manufacturing cost can be reduced as compared with the conventional case.

  As described above, according to the cryopreservation container 1 of the present embodiment, the label 10 can be prevented from being peeled off, and can be used in a field that requires a higher degree of cleanliness than before. Also, the manufacturing cost can be reduced.

(Cryopreservation container kit)
Next, the cryopreservation container kit 100 of this embodiment will be described. A cryopreservation container kit 100 of this embodiment includes a label 10 having a base material layer 11 and an adhesive layer 12 shown in FIG. 2 and a cryopreservation container 1 to which the label 10 is attached. As shown in FIG. 1, the cryopreservation container 1 has a label attaching region 1a to which a label 10 is attached to at least a part of the outer surface 1f. As described above, the label pasting region 1a has an arithmetic average roughness Ra of not more than the thickness t of the adhesive layer 12 and not less than 0.04 times the thickness t of the adhesive layer 12, and the ratio of the surface area to the unit flat area is as follows. 5 or more.

  According to the cryopreservation container kit 100 of the present embodiment, the adhesive layer 12 of the label 10 can be firmly adhered to the label attaching region 1a of the cryopreservation container 1 as described above. Accordingly, it is possible to prevent the label 10 from being peeled off due to moisture such as dew condensation or the like due to temperature changes during freezing or thawing of the contents of the cryopreservation container 1. In addition, the cryopreservation container 1 does not include a dust generation source or a fiber sheet that causes an increase in cost in the label attaching region 1a. Therefore, it can be used in fields requiring higher cleanliness than the conventional cryopreservation container 1, and the manufacturing cost can be reduced as compared with the conventional cryopreservation container 1.

  As described above, according to the cryopreservation container kit 100 of the present embodiment, the label 10 can be prevented from being peeled off, and can be used in a field that requires a higher degree of cleanliness than before. Manufacturing cost can be reduced.

(Base material for cryopreservation container)
Next, the base material 1m for cryopreservation containers of this embodiment is demonstrated. As shown in FIG. 2, the base material 1m for a cryopreservation container of this embodiment is a base material for manufacturing the cryopreservation container 1 to which the label 10 having the base material layer 11 and the adhesive layer 12 is attached. . The base material 1m for the cryopreservation container has a label attaching region 1a in which the label 10 is attached to at least a part of the outer surface 1f in advance before manufacturing the cryopreservation container 1 shown in FIG. As described above, the label pasting region 1a has an arithmetic average roughness Ra of not more than the thickness t of the adhesive layer 12 and not less than 0.04 times the thickness t of the adhesive layer 12, and the ratio of the surface area to the unit flat area is as follows. 5 or more.

  According to the cryopreservation container substrate 1m of the present embodiment, since the label pasting region 1a is formed in advance on at least a part of the outer surface 1f, the step of forming the label pasting region 1a after the cryopreservation container 1 is manufactured. As described above, the adhesive layer 12 of the label 10 can be firmly adhered to the label attaching region 1a of the cryopreservation container 1 after manufacture. Accordingly, it is possible to prevent the label 10 from being peeled off due to moisture such as dew condensation or the like due to temperature changes during freezing or thawing of the contents of the cryopreservation container 1. Moreover, the base material for cryopreservation containers does not contain the fiber sheet which becomes a dust generation source and a factor of a cost increase in the label sticking area | region 1a. Therefore, it can be used in fields requiring higher cleanliness than the conventional cryopreservation container 1, and the manufacturing cost can be reduced as compared with the conventional cryopreservation container 1.

  As described above, according to the cryopreservation container substrate 1m of this embodiment, it is possible to prevent the label 10 attached to the cryopreservation container 1 from being peeled off, and a higher degree of cleanliness than before is required. The manufacturing cost can be reduced as compared with the conventional method.

(Method for manufacturing cryopreservation container)
Finally, the manufacturing method of the cryopreservation container 1 of this embodiment is demonstrated. The cryopreservation container 1 can be manufactured, for example, by molding a resin material by inflation molding or injection molding. The resin material of the cryopreservation container 1 is not particularly limited as long as it is suitable for cryopreservation of cells and the like, and for example, polyethylene, ethylene / vinyl acetate copolymer resin, and the like can be used.

  FIG. 4 is a process diagram showing a manufacturing method of the cryopreservation container 1 shown in FIG. The manufacturing method of the cryopreservation container 1 according to the present embodiment includes a mold processing step S1 for forming an uneven area on the mold, and a surface shape of the uneven area of the mold on at least a part of the outer surface 1f of the cryopreservation container 1. And an uneven transfer step S2 for forming a label attaching region 1a to which the label 10 is attached by transfer.

  In the molding die processing step S <b> 1, an uneven region is formed on at least a part of a molding die used for injection molding of the cryopreservation container 1 and a molding die that performs hot embossing on the surface of the cryopreservation container 1 after molding. More specifically, for example, a blasting process is performed on a part or the whole of the mold for forming the surface shape of the cryopreservation container 1 to form unevenness, and then the uneven surface is formed by plating the uneven surface. Form. In the blasting treatment in which sand or metal fine particles collide with the surface of the mold, for example, fine particles of 0.01 μm or more and 1000 μm or less can be suitably used. The arithmetic average roughness Ra of the concavo-convex region tends to be smaller as the particles colliding in the blasting process are smaller or the collision energy is lower, and conversely, the arithmetic mean roughness Ra is larger as the particles are larger or the collision energy is higher. Further, the ratio of the surface area to the unit flat area of the concavo-convex region is smaller as the number of colliding particles is smaller, and conversely is larger as the number of particles is larger.

  Thereby, for example, the arithmetic average roughness Ra is not more than the thickness t of the pressure-sensitive adhesive layer 12 and not less than 0.04 times the thickness t of the pressure-sensitive adhesive layer 12 in a part or the whole of the mold, and the surface area per unit plane area A concavo-convex region having a ratio of 5 or more is formed. In addition, as a method of forming the uneven region on the mold, in addition to the blasting process, a chemical etching process, a plasma process, a corona process for etching a workpiece by applying a chemical reaction / corrosion action by a chemical such as an etchant. There are methods such as processing, laser processing, and cutting. In addition, a combination of photoresist techniques can also be used to produce a pattern-like uneven region such as a line shape or a lattice shape. Moreover, you may give metal plating, such as chromium, to an uneven | corrugated area | region as needed, in order to improve the intensity | strength of an uneven | corrugated area | region. As the material of the mold, for example, steel, ceramics, glass, or the like can be used.

  In the concavo-convex transfer step S2, the surface shape of the concavo-convex region of the mold is transferred to at least a part of the outer surface 1f of the cryopreservation container 1 to form a label attaching region 1a to which the label 10 is attached. The label pasting region 1a formed in the uneven transfer step S2 has an arithmetic average roughness Ra of not more than the thickness t of the adhesive layer 12 and not less than 0.04 times the thickness t of the adhesive layer 12, and a unit flat area The ratio of the surface area to is 5 or more.

  More specifically, for example, when the cryopreservation container 1 is molded by injection molding, the surface shape of the concavo-convex area is set to at least the outer surface 1f of the cryopreservation container 1 by using a mold in which the concavo-convex area is formed. Can be transcribed in part. Further, for example, hot embossing can be performed using a molding die in which an uneven region is formed on at least a part of the surface of the cryopreservation container 1 molded by inflation molding. Thereby, the surface shape of the uneven | corrugated area | region of a shaping | molding die can be transcribe | transferred to at least one part of the outer surface 1f of the cryopreservation container 1. FIG.

  Thus, the manufacturing method of the cryopreservation container 1 of this embodiment has the mold processing step S1 and the unevenness transfer step S2. Thereby, the arithmetic average roughness Ra is not more than the thickness t of the adhesive layer 12 and 0.04 times or more the thickness t of the adhesive layer 12, and the ratio of the surface area to the unit plane area is 5 or more. The cryopreservation container 1 having can be easily manufactured. Therefore, peeling of the label 10 affixed to the label affixing region 1a can be prevented, and it can be used in a field where a higher degree of cleanliness is required than before, and the manufacturing cost can be reduced as compared with the past. A cryopreservation container 1 can be provided.

  The embodiment of the present invention has been described in detail with reference to the drawings, but the specific configuration is not limited to this embodiment, and there are design changes and the like without departing from the gist of the present invention. They are also included in the present invention.

  Examples 1 and 2 of the cryopreservation container of the present invention and comparative examples of cryopreservation containers not included in the present invention will be described below.

Example 1
Based on the manufacturing method of the cryopreservation container described in the above embodiment, in the mold processing step, the entire surface of the mold is subjected to blasting to form irregularities, and then plated with chromium by electroplating, An uneven area was formed on the entire surface of the mold forming the outer surface of the cryopreservation container.

  The arithmetic average roughness Ra of the uneven area of the mold and the ratio of the surface area to an arbitrary unit plane area of 200 μm × 200 μm were measured using a laser microscope VK-9510 manufactured by Keyence Corporation. The arithmetic average roughness Ra Was 1.1 μm, and the ratio of the surface area to the unit plane area was 5.28. In addition, the ratio of the surface area to the arithmetic average roughness Ra and the unit flat area was calculated by obtaining the surface shape with a laser microscope at a magnification of 50 times and a measurement pitch in the depth direction of 0.1 μm. The reference length in the calculation of the arithmetic average roughness Ra was 200 μm.

  Next, a polyethylene substrate B300H manufactured by Ube Maruzen Polyethylene Co., Ltd. manufactured by inflation film formation was prepared. Four polyethylene substrates were prepared in the form of 20 mm square and 30 μm thick film. The polyethylene substrate is subjected to a heat embossing process using a mold having an uneven area, and an uneven transfer process is performed to transfer the surface shape of the uneven area to form a labeling area. Four specimens of the cryopreservation container were produced. Using the above laser microscope, the ratio of the arithmetic average roughness Ra of the labeling area of the test piece of Example 1 to the surface area of any unit plane area of 200 μm × 200 μm was measured in the same manner as the uneven area of the mold. However, the arithmetic average roughness Ra was 1.8 μm, and the ratio of the surface area to the unit plane area was 11.27.

(Example 2)
As in Example 1, in the mold processing step, the surface of the mold that forms the outer surface of the cryopreservation container by blasting the entire surface of the slide glass S2112 manufactured by Matsunami Glass Co., Ltd. to form irregularities An uneven area was formed throughout.

  In the same manner as in Example 1, the arithmetic average roughness Ra of the uneven area of the mold and the ratio of the surface area to an arbitrary unit plane area of 200 μm × 200 μm were measured using the above laser microscope. Was 1.1 μm, and the ratio of the surface area to the unit plane area was 4.86.

  In the same manner as in Example 1, a concavo-convex transfer process was performed on the polyethylene base material to form a label attaching region on the surface of the polyethylene base material, and four test pieces of the cryopreservation container of Example 2 were manufactured. When the arithmetic average roughness Ra of the labeling region of the test piece of Example 2 and the ratio of the surface area to an arbitrary unit flat area of 200 μm × 200 μm were measured, the arithmetic average roughness Ra was 0.8 μm, The ratio of surface area to area was 5.17.

(Comparative example)
Four of the polyethylene substrates used in Examples 1 and 2 that do not form a labeling region were prepared and used as test pieces for the cryopreservation container of Comparative Example 1. As in Examples 1 and 2, the arithmetic average roughness Ra of the surface of the polyethylene substrate and the ratio of the surface area to an arbitrary unit plane area of 200 μm × 200 μm were measured. The arithmetic average roughness Ra was 0.08 μm. Yes, the ratio of the surface area to the unit plane area was 2.36.

  Next, Teppura (registered trademark) tape SS24K manufactured by King Jim Co., Ltd. was prepared as a label to be attached to the cryopreservation container. It was 20 micrometers when the thickness of the adhesion layer of a label was measured with said laser microscope. That is, the label pasting area of the test piece of Example 1 has an arithmetic average roughness Ra of 0.09 times the thickness of the adhesive layer, and a ratio of the surface area to the unit flat area is 11.27. Moreover, the label pasting region of the test piece of Example 2 has an arithmetic mean roughness Ra of 0.04 times the thickness of the adhesive layer, and a ratio of the surface area to the unit flat area is 5.17. On the other hand, the surface of the test piece of the comparative example has an arithmetic average roughness Ra of 0.004 times the thickness of the adhesive layer, and the ratio of the surface area to the unit plane area is 2.36.

  The above-mentioned label was affixed to the label application region of each of the four test pieces of Examples 1 and 2 and the surface of the four test pieces of the comparative example. The size of the label was 10 mm square. Each test piece was immersed in liquid nitrogen, stored for one day, and returned to room temperature. Although the label affixed to the label application area | region of each four test pieces of Example 1 and 2 did not peel, the label affixed on the surface of the four test pieces of a comparative example peeled.

  Furthermore, even if each of the four test pieces of Examples 1 and 2 was bent so that the radius of curvature was 5 mm and cooled similarly to room temperature, the label attached to the label application region did not peel off, The labels attached to the surfaces of the four test pieces of the comparative examples were peeled off under the same conditions. As described above, the label is peeled off by the label affixing region in which the arithmetic average roughness Ra is not more than the thickness of the adhesive layer and not less than 0.04 times the thickness of the adhesive layer, and the ratio of the surface area to the unit flat area is 5 or more. It was confirmed that this can be prevented.

  Further, in the uneven transfer step, the ratio of the surface area to the arithmetic average roughness Ra and the unit flat area of the label application region is larger than the ratio of the surface area to the arithmetic average roughness Ra and the unit flat area of the uneven region of the mold. The tendency to become was seen. Further, in the uneven transfer process, depending on the material of the mold and the base material, the ratio of the surface area to the arithmetic average roughness Ra and unit flat area of the uneven area of the mold, and the arithmetic average roughness Ra and unit flat area of the labeling area It has been found that the relationship with the ratio of surface area to surface changes.

DESCRIPTION OF SYMBOLS 1 Cryopreservation container 1a Label sticking area | region 1f Outer surface 1m Base material 11 for cryopreservation containers Base material layer 12 Adhesive layer 10 Label 100 Cryopreservation container kit S1 Mold processing process S2 Concavity and convexity transfer process t Thickness of adhesion layer

Claims (7)

A cryopreservation container to which a label having a base material layer and an adhesive layer is attached,
Having a label affixing area where the label is affixed to at least a portion of the outer surface;
The label pasting region has an arithmetic average roughness Ra of not more than the thickness of the adhesive layer and not less than 0.04 times the thickness of the adhesive layer, and a ratio of a surface area to a unit plane area of 5 or more. And cryopreservation container.   The cryopreservation container according to claim 1, wherein the cryopreservation contents are filled and sealed.   The cryopreservation container according to claim 1 or 2, wherein the label is attached to the label application region.   The cryopreservation container according to claim 3, wherein the adhesive layer has a thickness of 10 μm or more. A cryopreservation container kit comprising a label having a base material layer and an adhesive layer, and a cryopreservation container to which the label is attached,
The cryopreservation container has a label attaching region where the label is attached to at least a part of an outer surface;
The label pasting region has an arithmetic average roughness Ra of not more than the thickness of the adhesive layer and not less than 0.04 times the thickness of the adhesive layer, and a ratio of a surface area to a unit plane area of 5 or more. A cryopreservation container kit. A base material for a cryopreservation container to which a label having a base material layer and an adhesive layer is attached,
Having a label affixing area where the label is affixed to at least a portion of the outer surface;
The label pasting region has an arithmetic average roughness Ra of not more than the thickness of the adhesive layer and not less than 0.04 times the thickness of the adhesive layer, and a ratio of a surface area to a unit plane area of 5 or more. A base material for a cryopreservation container. A method for producing a cryopreservation container to which a label having a base material layer and an adhesive layer is attached,
A mold processing step for forming an uneven region by attaching particles having an average particle diameter equal to or less than the thickness of the adhesive layer to at least a part of the mold; and
A concavo-convex transfer step of transferring a surface shape of the concavo-convex region of the mold to at least a part of the outer surface of the cryopreservation container to form a label attaching region to which the label is attached,
The label pasting region has an arithmetic average roughness Ra of not more than the thickness of the adhesive layer and not less than 0.04 times the thickness of the adhesive layer, and a ratio of a surface area to a unit plane area of 5 or more. A method for producing a cryopreservation container.

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