JP2017031111A - Skin quality improvement composition, and cosmetics - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new skin quality improvement composition which can improve the moisture retention of a skin, and cosmetics.SOLUTION: A skin quality improvement composition comprises (A) water-soluble polymers, and (B) sphingoglycolipids.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a skin quality improving composition and a cosmetic, and more particularly to a skin quality improving composition and a cosmetic that improve moisture retention.

Dry skin is skin in a state where the water content in the stratum corneum is reduced due to a decrease in sebum secretion or a decrease in intercellular stratum corneum lipids, and the function as a barrier is reduced.
Therefore, when the skin becomes dry, the moisture inside the skin evaporates and the skin further dries, or the resistance to irritation decreases and the skin develops into troubles such as rough skin.

  Therefore, various techniques have been studied so far in order to improve such dry skin or prevent the occurrence of dry skin.

For example, Patent Document 1 contains 0.0001 to 0.2% by weight of hyaluronic acid or a derivative thereof and 0.5-10% by weight of chitosan or a derivative thereof. Is disclosed.
Patent Document 2 discloses an emulsifier-type skin external preparation, the surface of which is treated with hyaluronic acid and / or a salt thereof which may be hydrolyzed, and a polymer or copolymer having a phosphorylcholine residue. An external preparation for skin characterized by containing the above.
Patent Document 3 discloses a moisturizing agent containing collagen, hyaluronic acid or / and a salt thereof, and trimethylglycine. When collagen is 1, the hyaluronic acid or / and the salt is 0.5. -3, a moisturizing agent having a mixing ratio (weight ratio) of trimethylglycine of 0.5 to 6 is disclosed.

JP 2007-99703 A JP 2013-53088 A JP 2014-129337 A

Since the compositions disclosed in Patent Documents 1 to 3 contain hyaluronic acid that exhibits a water-retaining effect, a certain effect can be expected with respect to improving the moisture retention of the skin.
However, the user's need for the moisture retention of the skin does not decrease and continues to increase. That is, the creation of a skin quality improving composition or cosmetic that exhibits a higher effect on the moisture retention of the skin is required.

  In addition, since the presence of a user who does not match the skin is assumed with a predetermined skin quality improving composition or cosmetic, it is necessary to present many options to the user. Therefore, it is also necessary to create a skin quality improving composition or a cosmetic having a combination of components different from the combination of components disclosed in Patent Documents 1 to 3.

  Then, this invention makes it a subject to provide the new skin quality improvement composition which can improve the moisture retention of skin, and cosmetics.

The above problem can be solved by the following means.
(1) A skin quality improving composition comprising (A) a water-soluble polymer and (B) a glycosphingolipid.
(2) The water-soluble polymer of the component (A) is at least one selected from the group consisting of hyaluronic acid, hyaluronic acid salt, hyaluronic acid derivative, carboxyvinyl polymer, hydroxyethyl cellulose, and carboxymethyl cellulose. 2. The skin quality improving composition as described in 1 above, wherein
(3) The value of the ratio of the total content of the component (B) and the component (C) with respect to the content of the component (A) is 0.02 to 10. 3. The skin quality improving composition according to 1 or 2 above, wherein the composition is 0.
(4) The content of the component (A) is 0.01 to 1.00% by mass, and the total content of the component (B) and the component (C) is 0.01 to 2. 4. The skin quality improving composition as described in 3 above, wherein the composition is 50% by mass.
(5) The skin quality improving composition as described in any one of (1) to (4) above, wherein the component (B) is derived from a microorganism.
(6) A cosmetic comprising the skin quality improving composition according to any one of (1) to (5).

  Since the skin quality improving composition according to the present invention includes (A) a water-soluble polymer and (B) a glycosphingolipid, the synergistic action by the combination of the component (A) and the component (B) It is possible to exert a very excellent effect on improving the moisture retention of the skin.

  In addition, the skin quality improving composition according to the present invention contains (C) a phospholipid and a value of the ratio of the total content of the (B) component and the (C) component to the content of the (A) component. However, since it is within the predetermined range, the effect of improving the moisture retention of the skin is ensured.

  In the skin quality improving composition according to the present invention, the content of the component (A) is in a predetermined range, and the total content of the component (B) and the component (C) is in a predetermined range. For this reason, the effect of improving the moisture retention of the skin is more certain.

  In the skin quality improving composition according to the present invention, the component (B) may be derived from a predetermined microorganism.

  Since the cosmetic according to the present invention includes the above-described skin quality improving composition, it can exhibit the effect of improving the moisture retention of the skin.

It is measurement data for confirming the influence which content of (B) component and (C) component has on the moisture increase amount of skin. It is the measurement data of the transmittance | permeability of each sample in case the (A) component whose average molecular weight is 2 million is used. It is the measurement data of the transmittance | permeability of each sample in case the (A) component whose average molecular weight is 1.2 million is used. It is the measurement data of the transmittance | permeability of each sample in case the (A) component whose average molecular weight is 100,000 is used. It is the measurement data of the transmittance | permeability of each sample in case the (A) component whose average molecular weight is 50,000 is used.

  Hereinafter, the form (embodiment) for implementing the skin quality improvement composition and cosmetics which concern on this invention is demonstrated.

≪Skin quality improving composition≫
The skin quality improving composition according to the present embodiment (hereinafter referred to as “composition” as appropriate) includes (A) a water-soluble polymer and (B) a glycosphingolipid. Moreover, it is preferable that the composition which concerns on this embodiment contains (C) phospholipid further.
And the composition which concerns on this embodiment should be used suitably as an external preparation which performs application | coating, sticking, a dispersion | spreading, etc. with respect to the skin where moisture retention is falling, or the skin which wants to prevent a moisture retention fall. Can do.
Hereinafter, each component contained in the composition according to the present embodiment will be described in detail.

<(A) component>
The composition according to this embodiment contains a water-soluble polymer as the component (A). This component (A) has a function of improving the moisture retention of the skin. In addition, 0.01-1.00 mass% is preferable, and, as for content of (A) component, 0.05-0.50 mass% is more preferable.

The water-soluble polymer (A) is a water-soluble polymer compound having a molecular weight of 1000 or more (including so-called quasi-polymer compounds). And as the water-soluble polymer, hyaluronic acid, hyaluronic acid salt, hyaluronic acid derivative, carboxyvinyl polymer, carboxyvinyl polymer salt, hydroxyethylcellulose, hydroxyethylcellulose salt, carboxymethylcellulose, and carboxymethylcellulose salt, and more It is preferably at least one selected from the group consisting of Further, among these, at least one selected from the group consisting of hyaluronic acid, a salt of hyaluronic acid, and a derivative of hyaluronic acid is more preferable.
The hyaluronic acid as component (A) is represented by the following structural formula.

Examples of the salt of hyaluronic acid (A) include alkali metal salts such as sodium and potassium, alkaline earth metal salts such as calcium and magnesium, and metal salts such as aluminum and zinc. Are preferably used.
The derivative of hyaluronic acid as component (A) is a compound produced by changing a small portion of hyaluronic acid, including propylene glycol hyaluronate, sodium acetylated hyaluronate, hydroxypropyltrimonium hyaluronate, hydrolyzed glyceryl hyaluronate, A hyaluronic acid crosspolymer etc. can be illustrated.

The carboxyvinyl polymer as the component (A) is an acidic polymer compound having a carboxyl group, and a main constituent monomer is a crosslinked polymer of acrylic acid.
As the component (A), a salt of a carboxyvinyl polymer, for example, an alkali metal salt such as sodium or potassium, an alkaline earth metal salt such as calcium or magnesium, or a metal salt such as aluminum or zinc may be used.

The (A) component hydroxyethyl cellulose is a cellulose ether obtained by partially hydroxyethylating a hydroxyl group of cellulose.
As the component (A), a salt of hydroxyethyl cellulose, for example, an alkali metal salt such as sodium or potassium, an alkaline earth metal salt such as calcium or magnesium, or a metal salt such as aluminum or zinc may be used.

The component (A) carboxymethylcellulose is a cellulose ether obtained by partially carboxymethylating a hydroxyl group of cellulose.
As the component (A), a salt of carboxymethyl cellulose, for example, an alkali metal salt such as sodium or potassium, an alkaline earth metal salt such as calcium or magnesium, or a metal salt such as aluminum or zinc may be used.

Although the average molecular weight of (A) component is not specifically limited, 100,000 or less are preferable and 10,000 or less are especially preferable. When the average molecular weight of the component (A) is not more than a predetermined value, when the component (A) is combined with the components (B) and (C) described later, the transparency of the entire composition (transparency after solution) It can be in a high state.
The average molecular weight of the component (A) is determined, for example, according to the term “viscosity” described in “Purified Sodium Hyaluronate” in the 15th Amendment Japanese Pharmacopoeia, and Laurent's formula (Formula 1). Can be used to calculate.
(Laurent et al., Biochim Biophys Acta. 1960 Aug 26; 42: 476-485.)
(Formula 1): Intrinsic viscosity (dL / g) = 0.00036 × M ^0.78

<(B) component>
The composition according to this embodiment contains glycosphingolipid as the component (B). This component (B) has the function of improving the moisture retention of the skin, like the component (A). In addition, 0.005-1.25 mass% is preferable and, as for content of (B) component, 0.05-1.0 mass% is more preferable.
The glycosphingolipid of component (B) is represented by the following structural formula.

R ₁ in the formula (1) is a sugar moiety composed of four hexoses or one uronic acid selected from the group consisting of uronic acid, glucosamine, galactose, and mannose. As for the four hexoses, the number of hexoses, the bonding order, the bonding form, and the optical isomerism are not particularly limited as long as one to four are combined from uronic acid, glucosamine, galactose and mannose. Examples of R ₁ combinations include those having uronic acid as the only hexose, and those consisting of four hexoses composed of uronic acid, glucosamine, galactose and mannose.

The following structures A and B can be illustrated as specific examples of R ₁ .

R ₂ in Formula (1) is an alkyl group, an alkenyl group, or an alkynyl group that may have a cycloalkyl group. The number of carbon atoms of R ₂ is not particularly limited, but is preferably in the range of 15 to 25. The alkyl group, alkenyl group and alkynyl group of R ₂ may be linear or branched and may or may not be substituted with a hydroxyl group or the like. In particular, a cycloalkyl group such as a cyclopropyl group may be present in the chain of the alkyl group. The position of the double bond of the alkenyl group and the position of the triple bond of the alkynyl group are not particularly limited.

The following structures a to c can be exemplified as specific examples of R ₂ .

R ₃ in the formula (1) is an alkyl group. The alkyl group that R ₃ can take may be linear or branched, and may or may not be substituted with a hydroxyl group or the like. The carbon number of the alkyl group is usually in the range of 1 to 50, and preferably in the range of 15 to 25. As a specific example of R ₃ , a linear alkyl group having 12 carbon atoms can be exemplified.

As a group of glycosphingolipids preferably used in the composition of the present invention, R _{1 in} formula (1) is a sugar moiety represented by structures A and B, and R ₃ is a linear alkyl group having 12 carbon atoms. The glycosphingolipid group which is can be mentioned. Another preferred glycosphingolipid group is a glycosphingolipid group in which R _{2 in} formula (1) is represented by structures a, b, or c, and R ₃ is a linear alkyl group having 12 carbon atoms. it can. As a particularly preferred glycosphingolipid group, R ₁ in formula (1) is a saccharide moiety represented by structures A and B, R ₂ is represented by structures a, b, or c, and R ₃ is 12 carbon atoms. A glycosphingolipid group which is a linear alkyl group can be exemplified.

  These glycosphingolipids represented by the formula (1) may be contained alone in the composition of the present invention, or may be contained in combination of two or more. The ratio of each component in the case of containing 2 or more types in combination is not particularly limited.

<(C) component>
The composition according to this embodiment preferably contains a phospholipid as the component (C). The component (C) has a function of improving the stability of the composition (stability after solution) by coexisting with the component (A) and the component (B). In addition, 0.005-1.25 mass% is preferable and, as for content of (C) component, 0.05-1.0 mass% is more preferable.

  In addition, although the phospholipid of (C) component is an ester of phosphoric acid and a fatty acid, specifically, when (B) component is produced using a microorganism of the genus Sphingomonas described later, It is a cell membrane phospholipid produced.

<Value of ratio of total content of component (B) and component (C) to content of component (A)>
When the composition which concerns on this embodiment contains not only (A) component and (B) component but (C) component, with respect to content of (A) component, (B) component and (C) component The total content ratio value (= {(B) component content + (C) component content} / (A) component content: hereinafter referred to as “content ratio” where appropriate)) is 0.02. It is preferably ˜10.0, more preferably 0.2 to 5.0.
When the value of this content ratio is within a predetermined range, the effect of improving the moisture retention of the skin obtained by the synergistic action of the component (A), the component (B), and the component (C) is certain. Become.

  Note that the ratio of the content of the component (C) to the content of the component (B) (= content of the component (C) / content of the component (B)) is not particularly limited, but is described later in the genus Sphingomonas What is necessary is just to set it as the ratio of content of (C) component with respect to content of (B) component at the time of producing using this microorganism, for example, 0.5-1.5.

  The content of each component can be measured by commercially available high performance liquid chromatography (HPLC), thin layer chromatography (TLC), or the like.

In addition, when (B) component and (C) component are produced using microorganisms of the genus Sphingomonas described later, the average particle size of (B) component and (C) component is about 15 μm. By applying, it is preferable to use the component (B) and the component (C) having an average particle diameter of 60 to 80 nm. By using the small component (B) and component (C) having an average particle size of about 60 to 80 nm, the effect of improving the transparency of the entire composition (transparency after solution) can be ensured, and the composition The increase in viscosity (viscosity after solution) can be suppressed.
And in order to make the average particle diameter of (B) component and (C) component 60-80 nm, what is necessary is just to give a high-pressure process (pressure: 90-95 MPa) with respect to (B) component and (C) component. .
In addition, the average particle diameter of (B) component and (C) component can be measured by nanoparticle diameter measurement distribution apparatus SALD-7500 nano (made by Shimadzu Corporation).

<Others>
The composition according to the present embodiment may be in any form such as a solid, liquid, paste, jelly, and powder as long as it is suitably used as a cosmetic.
In addition, when making the composition which concerns on this embodiment into solid form, excipient | fillers, such as a dextrin, a cellulose, lactose, vitamin B2, vitamin B6, vitamin B12, ascorbine other than an amino acid, a nucleic acid, collagen, acetylglucosamine, etc. It may contain vitamins such as acids or salts thereof, minerals such as iron and calcium, other lubricants, fragrances, etc., and in the case of liquid form, further thickeners such as alginic acid or salts thereof and xanthan gum Further, emulsifiers such as sucrose fatty acid ester and glycerin fatty acid ester, and pH adjusting agents such as sodium hydrogen carbonate and citric acid may be contained.
In addition, the composition according to this embodiment may contain substances used for each cosmetic described later.

Next, the cosmetic containing the skin quality improving composition according to the above-described embodiment will be described.
≪Cosmetics≫
The cosmetic according to the present embodiment is a cosmetic containing the composition according to the present embodiment. And about the content ratio and content of (A) component in the cosmetics which concern on this embodiment, (B) component, and (C) component, it is each so that it may become the same content ratio and content as the said composition. It preferably contains components.
Moreover, if the cosmetics which concern on this embodiment are products for improving the moisture retention of skin, a form will not be specifically limited. Therefore, the cosmetic according to the present embodiment may be in any form such as a solid, a liquid, a paste, a jelly, and a powder. In order to form such a form, for example, it is solidified using a gelling agent, is dispersed using a liquid, is made into a solution by adding a solvent, and is spray-dried into a powder form. do it.

And as the types of cosmetics according to the present embodiment, for example, cosmetic soap, shampoo, facial cleanser, rinse, eye cream, eye shadow, cream / milky lotion, lotion, beauty liquid, perfume, funny, cosmetic oil, hair Cosmetics, hair dyes, perfumes, powders, packs, shave creams, shave lotions, tanning oils, sunscreen oils, sunscreen lotions, sunscreen lotions, tanning creams, sunscreen creams, foundations, powdered perfumes, blushers, Examples include mascara, eyebrow, nail cream, nail enamel, beauty nail enamel remover, hair wash, bath cosmetic, lipstick, lip balm, eyeliner, toothpaste, deodorant, eau de cologne, hair restorer, and hair restorer. It can also be used as an ointment or poultice.
In addition, since the cosmetic according to the present embodiment is not only excellent in moisture retention but also excellent in transparency, among the cosmetics described above, products that require high transparency and no coloring are advantages. It can be suitably applied to products such as lotions and cosmetic liquids.

  Various components may be added to the cosmetic according to the present embodiment depending on the purpose of use. Examples of the additive component include oil and fat components, UV absorbers, IR absorbers, emulsifiers, surfactants, antiseptics, antifungal agents, antioxidants, whitening agents, polysaccharides, fluorescent materials, pigments, dyes, fragrances, Examples include sequestering agents, binders, extenders, antibiotics, blood circulation promoters, anti-inflammatory agents, cell activators, and the like. Moreover, the moisturizing component other than the component (A), the component (B), and the component (C) can be further added to the cosmetic according to the present embodiment.

  Fatty acids (for example, oleic acid, behenic acid, isostearic acid, lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, linoleic acid, γ-linolenic acid can be used as the fat and oil component that can be used in the cosmetic according to this embodiment. Acid, columbic acid, eicosa- (n-6,9,13) -trienoic acid, arachidonic acid, α-linolenic acid, thymnodonic acid, hexaenoic acid), ester oil (eg pentaerythritol-tetra-2-ethylhexanoate) , Isopropyl myristate, butyl stearate, hexyl laurate, octyldodecyl myristate, diisopropyl adipate, diisopropyl sebacate), wax (eg beeswax, whale wax, lanolin, carnauba wax, candelilla wax, petrolatum), animal oils and vegetable oils (eg mink Oil, olive oil, castor oil, cocoa butter, palm oil, cod liver oil, beef tallow, butter fat, evening primrose oil, rice bran oil, squalane), mineral oil (eg hydrocarbon oil, liquid paraffin), silicone oil (eg methylphenyl silicone) Dimethyl silicon), higher alcohols (for example, lauryl alcohol, stearyl alcohol, oleyl alcohol, cetyl alcohol, 2-octyldodecanol, 2-decyltetradecanol) and derivatives thereof. Moreover, (alpha) -hydroxy acid, hydroxycarboxylic acid, dicarboxylic acid, glycyrrhizic acid, glycyrrhetic acid, mevalonic acid (mevalonolactone) etc. can be used as an organic acid.

  Examples of UV absorbers that can be used in the cosmetic according to this embodiment include oxybenzone (2-hydroxy-4-methoxybenzophenone), oxybenzonesulfonic acid, oxybenzonesulfonic acid (trihydrate), guaiazulene, ethylene glycol salicylate, and satylyl. Octyl acid, dipropylene glycol salicylate, phenyl salicylate, homomenthyl salicylate, methyl salicylate, methyl diisopropylcinnamate, cinoxalate (2-ethoxyethyl p-methoxycinnamate), mono-2-ethylhexyl di-p-methoxycinnamate Glyceryl acid, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone, tetrahydroxybenzophenone, p-aminobenzoic acid , Ethyl p-aminobenzoate, glyceryl p-aminobenzoate, amyl p-dimethylaminobenzoate, 2-ethylhexyl p-dimethylaminobenzoate, p-hydroxyanisole, 2-ethylhexyl p-methoxycinnamate, p- Isopropyl methoxycinnamate, diisopropyl cinnamate, 2- (2-hydroxy-5-methylphenyl) benzotriazole, sodium hydroxymethoxybenzophenonesulfonate, 4-tert-butyl-4'-methoxybenzoylmethane, salicylic acid-2 -Ethylhexyl, glyceryl-p-minobenzoate, methyl orthoaminobenzoate, 2-hydroxy-4-methoxybenzophenone, amyl-p-dimethylaminobenzoate, 2-phenylbenzimidazole-5-sulfone 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, dicaroyl trioleate, p-methoxysilicic acid-2-ethoxyethyl, butylmethoxybenzoylmethane, glyceryl-mono-2-ethylhexanoyl-di-p- Examples thereof include methoxybenzophenone, 2-ethylhexyl-2-cyano-3,3-diphenyl acrylate, 2,2-dihydroxy-4-methoxybenzophenone, and ethyl-4-bishydroxypropylaminobezoate.

  Examples of emulsifiers and surfactants that can be used in the cosmetic according to this embodiment include nonionic surfactants, anionic surfactants, and cationic surfactants. Nonionic surfactants include sorbitan esters (eg sorbitan monolaurate, sorbitan monooleate, sorbitan monoisostearate), polyoxyethylene sorbitan esters (eg polyoxyethylene sorbitan monoisostearate, polyoxyethylene sorbitan monolaurate) , Polyoxyethylene sorbitan monooleate), glycerol ether (eg glycerol monoisostearate, glycerol monomyristate), polyoxyethylene glycerol ether (eg polyoxyethylene glycerol monoisostearate, polyoxyethylene glycerol monomyristate) Polyglycerin fatty acid esters (eg diglyceryl monostearate, decaglyceryl decaisostearate, Glyceryl diisostearate), glyceryl fatty acid esters (eg glyceryl monocaprate, glyceryl monolaurate, glyceryl monomyristate, glyceryl monopalmitate, glyceryl monooleate, glyceryl monostearate, glyceryl monolinoleate, glyceryl monoisostearate) Rate, glyceryl monodilinoleate, glyceryl monodica plate), polyoxyethylene glyceryl fatty acid ester (eg, polyoxyethylene glyceryl monomyristate, polyoxyethylene glyceryl monooleate, polyoxyethylene glyceryl monostearate), polyoxyethylene Branched alkyl ethers such as polyoxyethylene octyldodecyl alcohol, polyoxyethylene-2-decyltetradec Alcohol), polyoxyethylene alkyl ether (eg, polyoxyethylene oleyl alcohol ether, polyoxyethylene cetyl alcohol ether), polyoxyethylene hydrogenated castor oil fatty acid ester (eg, polyoxyethylene hydrogenated castor oil, polyoxyethylene dihydrocholesterol ether, Polyoxyethylene hydrogenated castor oil isostearate), polyoxyethylene alkylaryl ether (for example, polyoxyethylene octylphenol ether) and the like can be exemplified.

  In addition, as an anionic surfactant, salts of higher fatty acids (eg oleic acid, stearic acid, isostearic acid, palmitic acid, myristic acid, behenic acid) (eg diethanolamine salt, triethanolamine salt, amino acid salt, potassium salt, sodium) Salt), ether carboxylic acid alkali salt, N-acyl amino acid salt, N-acyl sarcon salt, and higher alkyl sulfonate. Furthermore, examples of the cationic surfactant or amphoteric surfactant include alkyl quaternary ammonium salts, polyamines, and alkylamine salts.

  As powders that can be used in the cosmetic according to this embodiment, talc, kaolin, fuller's earth, rubber, starch, silica, silicic acid, aluminum silicate hydrate, chemically modified aluminum magnesium silicate, sodium polyacrylate, tetra Examples include alkylarylammonium succinate, trialkylarylammonium succinate, ethylene glycol monostearate, sodium carboxymethylcellulose, carboxyvinyl polymer, chalk, gum, ethylene glycol monostearate, ethylene glycol distearate it can.

  As polyols that can be used in the cosmetic according to the present embodiment, polyglycerin such as glycerin, diglycerin, triglycerin, tetraglycerin, ethylene glycol, propylene glycol, 1,3-butylene glycol, 1,4-butylene glycol , Dipropylene glycol, polyethylene glycol, sorbitol, erythritol, maltotriose, threitol, sucrose, glucose, maltose, maltose, fructose, and xylitol.

  As other materials that can be used in the cosmetic according to the present embodiment, vitamins (for example, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin K), amino acids (Eg proline, leucine, isoleucine, alanine, threonine, lysine, cysteine, arginine), hormones (eg follicular hormone, pregnenolone, corticosteroids), peptides (eg keratin, collagen, elastin), sugars (eg polyol) Exemplified), inorganic salts (for example, sodium chloride, sodium bicarbonate, sodium carbonate, borax, sodium sulfate, sodium sulfide, sodium thiosulfate, sodium sesquicarbonate, magnesium oxide, calcium carbonate, magnesium carbonate) , Potassium chloride, potassium sulfide), lactic acid bacteria culture, sterols (eg, cholesterol, provitamin D3, campesterol, stigmasterol, stigmasterol, 5-dihydrocholesterol, α-spinasterol, cholesterol fatty acid ester), sphingosines (E.g. sphingosine, dihydrosphingosine, phytosphingosine, dehydrosphingosine, dehydrophytosphingosine, sphingadienin), ceramide, pseudoceramide, saponin, chitin derivative, oligosaccharide (e.g. maltose, xylobiose, isomantose, lactose, sucrose, raffinose, Maltotriose, xylotriose, maltotetraose, xylotetraose, maltopentaose, xylopentaose , Maltohexaose, xylohexaose, maltoheptaose, xyloheptaose), acidic mucopolysaccharide (hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparin, heparan sulfate), and yeast extract.

  Furthermore, the cosmetics according to the present embodiment include thickeners (for example, electrolytes such as carboxyvinyl polymer, carboxymethylcellulose, polyvinyl alcohol, carrageenan, alginates, propylene glycol alginate, gelatin, sodium chloride), whitening agents (for example, Arbutin, Allantoin, Vitamin E derivative, Glycyrrhizin, Ascorbic acid phosphate, Magnesium salt, Kojic acid, Panthelic acid derivative, Placenta extract, Yakuinin, Green tea, Kakkon, Mulberry white skin, Licorice, Ogon, Aloe, Orange peel, Chamomile, Spirit Turf), skin protectants (eg retinol, retinol esters, retinoic acid), emollients (eg stearyl alcohol, glyceryl monoricinoleate, mink oil, cetyl alcohol, stearic acid, coconut oil, Skin oil, oisostearic acid), emollients (eg stearyl alcohol, glyceryl monoricinoleate, glyceryl monostearate, cetyl alcohol), skin permeation enhancers (eg 2-methylpropan-2-ol, 2-propanol, ethyl) -2-hydroxypropanoate, 2,5-hexanediol, acetone, tetrahydrofuran), bioactive plant extracts (eg, extracts of aloe, arnica, licorice, sage, assembly, etc.), preservatives (eg, p-hydroxybenzoic acid) Acid esters, sodium benzoate, urea, methylparaben, ethylparaben, propylparaben, butylparaben), anti-inflammatory agents (eg salicylic acid), bactericides (eg triclosan), antioxidants (eg α-tocopherol, butylhydroxytoluene), Loose Impactant (eg, triethanolamine or a combination of sodium hydroxide and lactic acid), keratolytic agent (eg, lactic acid, glycolic acid, malic acid, tartaric acid, citric acid), scrub agent (eg, polyethylene powder), pigment (eg, calcium, barium) And aluminum lake, iron oxide, titanium dioxide, mica) and the like. Materials other than these can also be added to the cosmetic of the present invention depending on the application. About the addition amount and addition method of each component, it can follow the method well-known in this technical field.

Next, the skin quality improving composition according to the above-described embodiment and the method for producing a cosmetic will be described. In addition, although (A) component contained in the skin quality improvement composition which concerns on this embodiment, (B) component, (C) component can also use a commercial item, it manufactures according to the following manufacture examples, for example. You can also
≪Production example≫
The method for producing a skin quality improving composition according to the present embodiment includes a microorganism culture step, a separation / purification step, and a mixing step.
As a production example, the case where the component (A) is hyaluronic acid or a hyaluronic acid salt will be described.

[Microbe culture process]
The microorganism culturing step is a step of causing a microorganism to produce the component (A), the component (B), and the component (C) by culturing a predetermined microorganism.
The microorganism for producing the component (A) used in the microorganism culturing step is not particularly limited as long as it has hyaluronic acid production ability, but a microorganism belonging to the genus Streptococcus (for example, Streptococcus zooepidemicus) can be preferably used.
The microorganism that produces the component (B) is not particularly limited as long as it has the ability to produce glycosphingolipids, but microorganisms belonging to the genus Sphingomonas can be preferably used.
The component (C) is produced together with the component (B) as a secondary component when a microorganism of the genus Sphingomonas is used as the microorganism for producing the component (B).
The composition of the culture solution used in the microorganism culture step, the sterilization conditions of the culture solution, and the culture conditions (temperature, time, etc.) may be performed under known conditions.

Specifically, when Sphingomonas paucimobilis is used as the microorganism of the genus Sphingomonas that produces component (B), R ₁ in formula (1) is generally structure A or structure B. , R ₂ is the structure a, and R ₃ is a straight-chain alkyl group having 12 carbon atoms.

[Separation and purification process]
The separation / purification step is a step of separating and purifying microorganisms and the like from the culture solution.
The method for separating microorganisms and the like is not particularly limited, and may be performed by a method such as filtration or centrifugation. Further, the method for purifying microorganisms and the like is not particularly limited, and may be performed by a known method.

[Mixing process]
The mixing step is a step of mixing the component (A), the component (B), and the component (C) obtained in the separation / purification step.
In this mixing step, each component may be mixed so as to have the above-described content ratio and content.

  If you want to use hyaluronic acid as component (A), separate the acid treatment step by adding alcohol or hydrous alcohol containing hydrochloric acid to the hyaluronic acid Na obtained in the separation / purification step, followed by washing and drying. -It may be performed after the purification step.

  Regarding the method for producing a cosmetic according to the present embodiment, the skin quality improving composition produced by the above-described method is mixed with a cosmetic material, and thereafter, the cosmetic is produced by a method for producing a cosmetic known to those skilled in the art. That's fine.

  Next, the skin condition improving composition and cosmetics according to the present invention will be described by exemplifying examples that satisfy the requirements of the present invention and comparative examples that are not.

Example 1
First, in Example 1, the influence which the content ratio and content of (A) component, (B) component, and (C) component have on "moisturizing property" is confirmed.

<Preparation of sample>
(A) component, (B) component, (C) component, phenoxyethanol (0.4 mass%) as a preservative, and purified water (remainder) at room temperature so that it may become the compounding quantity shown in the following Table 1 Each sample was prepared by mixing at.
And the component (A) used sodium hyaluronate (FCH-200, manufactured by Kikkoman Biochemifa Co., Ltd., molecular weight 2 million). Biosphingo (manufactured by Kikkoman Biochemifa Corporation) was used as the component (B) and the component (C). In this biosphingo, the content ratio of the component (B) to the component (C) was about 1: 1. The component (B) is a glycosphingolipid in which R ₁ in the formula (1) is the structure A or B, R ₂ is the structure a, and R ₃ is a linear alkyl group having 12 carbon atoms. , (C) component was phospholipid.
In addition, (B) component and (C) component prepared the aqueous solution of biosphingo, and then performed high pressure treatment (pressure: 90-95 MPa, number of passes: 3 times) using a high pressure homogenizer (APV-1000, manufactured by APV). ) Was used.

<Test content: moisture retention>
The skin moisture content of the forearm of 9 male and female subjects was measured using Corneometer CM825 (Courage + Khazaka Electronic GmbH) in an atmosphere with a room temperature of 19.6 to 22.9 ° C. and a humidity of 44.3.0 to 51.3%. Measured. Then, the said sample was apply | coated (20 microliters / 2cm < ² >) with respect to the skin after measuring the moisture content, and after 120 minutes passed, the skin moisture content was measured again.
For each subject, the amount of water increase (electric capacity AU) is calculated by subtracting the amount of skin moisture before applying the sample from the amount of moisture in the skin after applying the sample, and the average value of the calculated water increase is calculated. did.
The amount of increase in moisture, that is, the result of improvement in moisture retention is shown in FIG.

<Examination of test results>
As shown in FIG. 1, the sample (sample No. 1) that does not contain the component (B) and the component (C) does not have a large amount of water increase, and the effect of improving the moisture retention is not sufficient.
On the other hand, it was confirmed that the samples (samples Nos. 2 to 5) containing the component (B) and the component (C) had a large value of the amount of increase in moisture, and the effect of improving the moisture retention was great.
In particular, a sample in which the ratio of the total content of the component (B) and the component (C) with respect to the content of the component (A) falls within the range of 0.2 to 5.0 (sample No. 2 5), it was confirmed that the value of the amount of increase in water was considerably large, and the effect of improving the moisture retention was very large.

<< Example 2 >>
Next, in Example 2, the influence of the average molecular weight of the component (A) on “transparency” will be confirmed.

<Preparation of sample>
Components (A), (B), (C), phenoxyethanol (0.4% by mass) as preservatives, and purified water (remainder) at room temperature so that the blending amounts shown in FIGS. Each sample was prepared by mixing at.
For component (A), sodium hyaluronate (FCH-200, manufactured by Kikkoman Biochemifa Co., Ltd., molecular weight 2 million) was used in the test shown in FIG. 2, and sodium hyaluronate (FCH--) was used in the test shown in FIG. In the test shown in FIG. 4, using sodium hyaluronate (FCH-SU, manufactured by Kikkoman Biochemifa Corporation, molecular weight 100,000), the test shown in FIG. Then, micro hyaluronic acid (FCH, manufactured by Kikkoman Biochemifa Co., Ltd., molecular weight: 50,000) was used.
Biosphingo (manufactured by Kikkoman Biochemifa Corporation) was used as the component (B) and the component (C). In this biosphingo, the content ratio of the component (B) to the component (C) was about 1: 1. The component (B) is a glycosphingolipid in which R ₁ in the formula (1) is the structure A or B, R ₂ is the structure a, and R ₃ is a linear alkyl group having 12 carbon atoms. , (C) component was phospholipid.
In addition, (B) component and (C) component prepared the aqueous solution of biosphingo, and then performed high pressure treatment (pressure: 90-95 MPa, number of passes: 3 times) using a high pressure homogenizer (APV-1000, manufactured by APV). ) Was used.

<Test content: Transparency>
The transmittance (% T) of the sample was measured using an ultraviolet-visible spectrophotometer (V-630, manufactured by JASCO Corporation).

<Examination of test results>
As shown in FIGS. 2-5, it has confirmed that the transmittance | permeability fell as content of (A) component increased. Moreover, it has confirmed that the transmittance | permeability fell as content of (B) component and (C) component increased.
However, as is clear when comparing FIGS. 2 to 5, the lower the average molecular weight of the component (A), the lower the transmittance due to the increase in the content of the component (A), and the components (B) and (C). It was confirmed that the decrease in transmittance due to the increase in content can be suppressed.
In particular, as shown in FIG. 5, when the average molecular weight of the component (A) reaches 50,000, the transmittance is almost lowered even if the content of the components (A), (B), and (C) is increased. I confirmed that I did not.

Example 3
Next, in Example 3, the influence of the addition of the component (C) on “stability” is confirmed.

<Preparation of sample>
(A) component, (B) component, (C) component, phenoxyethanol (0.4 mass%) as a preservative, and purified water (remainder) at room temperature so that it may become the compounding quantity shown to Table 2, 3. Each sample was prepared by mixing at.
In addition, about component (A), sodium hyaluronate (FCH-200, manufactured by Kikkoman Biochemifa Co., Ltd., molecular weight 2 million), sodium hyaluronate (FCH-120, manufactured by Kikkoman Biochemifa Co., Ltd., molecular weight 1.2 million), micro hyaluronic acid Any of acids (FCH, manufactured by Kikkoman Biochemifa Co., Ltd., molecular weight: 50,000) was used.
Moreover, about the sample shown in Table 2, Biosphingo (Kikkoman Biochemifa Co., Ltd. product) was used as (B) component and (C) component. In this biosphingo, the content ratio of the component (B) to the component (C) was about 1: 1. The component (B) is a glycosphingolipid in which R ₁ in the formula (1) is the structure A or B, R ₂ is the structure a, and R ₃ is a linear alkyl group having 12 carbon atoms. , (C) component was phospholipid.
On the other hand, for the sample shown in Table 3, as a component (B), a glycosphingolipid (R _{1 of} formula (1)) obtained by excluding the component (C) from Biosphingo (manufactured by Kikkoman Biochemifa Co., Ltd.). Is structure A or structure B, R ₂ is structure a, and R ₃ is a glycosphingolipid having a linear alkyl group having 12 carbon atoms.
The components (B) and (C) shown in Table 2 are prepared by preparing an aqueous biosphingo solution and then using a high-pressure homogenizer (APV-1000, APV-1000) for high-pressure treatment (pressure: 90 to 95 MPa, pass (Number of times: 3 times).
Moreover, also about (B) component shown in Table 3, after adjusting aqueous solution, what was high-pressure-processed (pressure: 90-95 MPa, the frequency | count of a pass: 3 times) using the high-pressure homogenizer (APV company make, APV-1000). Was used.

<Test content: Stability>
The state of the sample after the lapse of 1 week from production (1 week standing at 25 ° C.) was visually confirmed, and the stability (stability after solution) was evaluated.
The evaluation criteria are as follows.
◯: No precipitate was confirmed, and the liquid was kept in a uniform state.
Δ: Some precipitates were confirmed.
X: Many precipitates were confirmed, and the liquid and the precipitates were clearly separated.

<Examination of test results>
As shown in Table 3, in the case of the combination of the component (A) + the component (B) (including the case where the content of the component (A) is 0% by mass), precipitates are confirmed for all the samples. (Evaluation was △).
On the other hand, as shown in Table 2, in the case of the combination of the component (A) + the component (B) + the component (C) (including the case where the content of the component (A) is 0% by mass), partly precipitated Although there was a sample in which an object was confirmed, many samples maintained a uniform state as a liquid (evaluation was Δ or ◯).
And since the result of Table 2 compared with the result of Table 3 and the stability became a favorable result as a whole, by further combining the component (C) with the component (A) + (B), It was confirmed that stability (stability after solution) was improved.

  Although the skin quality improving composition and cosmetics according to the present invention have been described in detail with reference to the embodiments and examples, the gist of the present invention is not limited to the above-described contents, and Needless to say, modifications and changes can be made based on the description.

Claims (6)

  A skin quality improving composition comprising (A) a water-soluble polymer and (B) glycosphingolipid.   The water-soluble polymer of the component (A) is at least one selected from the group consisting of hyaluronic acid, hyaluronic acid salt, hyaluronic acid derivative, carboxyvinyl polymer, hydroxyethyl cellulose, and carboxymethyl cellulose. The skin quality improving composition according to claim 1. (C) containing phospholipids,
The value of the ratio of the total content of the component (B) and the component (C) to the content of the component (A) is 0.02 to 10.0. Or the skin quality improvement composition of Claim 2.   The content of the component (A) is 0.01 to 1.00% by mass, and the total content of the component (B) and the component (C) is 0.01 to 2.50% by mass. The skin quality improving composition according to claim 3, wherein   The said (B) component is a microorganism-derived thing, The skin quality improvement composition of any one of Claims 1-4 characterized by the above-mentioned.   A cosmetic comprising the skin quality improving composition according to any one of claims 1 to 5.

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