JP2017019106A - Packaging material for packaging powder composition containing cysteine protease - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a package material capable of suppressing reduction of enzyme activities of cysteine protease contained in a powder composition even after including the powder composition containing cysteine protease in the package material.SOLUTION: There is provided a package material for packaging a powder composition containing cysteine protease, wherein the package material has a barrier layer, a sealant layer and a metallocene polyethylene layer, the sealant layer and the metallocene polyethylene layer are laminated in contact with each other and the sealant layer is a polyethylene layer or a non-drawing polypropylene layer.SELECTED DRAWING: None

Description

  The present invention includes a packaging material for packaging a powder composition containing cysteine protease, a packaging bag formed by molding the packaging material into a bag shape, and a powder composition containing cysteine protease enclosed in the packaging bag. It relates to the package.

  Tongue moss is formed when food residues, exfoliated oral mucosal cells, salivary components (proteins, etc.), bacteria, bacterial metabolites, etc. adhere to the tongue surface, and is considered to cause halitosis. ing.

  There are small protrusions (襞) called tongue papillae on the surface of the tongue, and tongue coating is formed by adhering and accumulating in the gaps of the tongue papillae, etc., which is sufficient for daily oral care such as tooth brushing and gargle It is difficult to remove. Therefore, as a method for removing tongue coating, for example, there is a method of physically removing tongue coating by brushing the tongue surface using a dedicated removal tool such as a tongue brush, or a method of chemically degrading tongue coating with an enzyme. Are known.

  Proteases are known as enzymes having a resolution of tongue coating, and foods such as candy and tablets containing enzymes such as cysteine protease have been reported as specific forms (Patent Documents 1 to 3).

  However, cysteine protease is heat-sensitive, has poor stability over time in high-temperature and high-humidity conditions such as summer, and easily deactivates, and stability over time of enzyme activity in compositions containing water such as aqueous solutions and aqueous gels. Is known to be bad. Therefore, for products containing cysteine protease, efforts such as devising compounding ingredients and devising a storage environment are required to prevent inactivation.

International Publication No. 03/090704 International Publication No. 2007/026755 International Publication No. 2007/105661

  As a result of research so far, the present inventors have improved the time-dependent stability of the enzyme activity of the cysteine protease and the powder composition for preparing the tongue coating removal gel at the time of use as a product for removing tongue coating by cysteine protease. The powder composition for removing tongue coating was developed, and patent applications have been filed for these compositions (Japanese Patent Application Nos. 2015-090703 and 2015-106416, both unpublished).

  In order to preserve and / or distribute these powder compositions, the present inventor has been studying a packaging bag in which the powder composition is enclosed. The powder composition is enclosed in a packaging bag and is moved up and down several times. When the powder composition is taken out after shaking and the enzyme activity of the cysteine protease contained in the powder composition is measured, it has been surprisingly found that the enzyme activity of the cysteine protease decreases.

  Although the reason for the phenomenon described above is not necessarily clear, the present inventors include cysteine protease contained in the powder composition adsorbed on the inner wall of the packaging bag and contained in the powder composition taken out from the packaging bag. It is speculated that a decrease in the amount of cysteine protease may result in a decrease in the enzyme activity of the cysteine protease contained in the powder composition.

  The present invention has been made in view of the above-mentioned problems, and even after a powder composition containing cysteine protease is sealed in a packaging bag, the enzyme activity of cysteine protease contained in the powder composition is reduced. It aims at providing the packaging material which can suppress.

  This inventor repeated earnest examination in order to solve an above-described subject. As a result, by using a packaging bag formed by molding a packaging material having a specific laminated structure into a bag shape, it is included in the powder composition even after the powder composition containing cysteine protease is sealed. It has been found that a decrease in the enzyme activity of cysteine protease can be suppressed. Based on this knowledge, the present inventor has completed the present invention through further research.

  That is, the present invention typically includes the subject matters described in the following sections.

Item 1.
A packaging material for packaging a powder composition containing cysteine protease,
The packaging material has a sealant layer, a metallocene polyethylene layer, and a barrier layer, and is laminated so that the sealant layer and the metallocene polyethylene layer are in contact with each other,
The sealant layer is a polyethylene layer or an unstretched polypropylene layer,
Packaging material.
Item 2.
Item 2. The packaging material according to Item 1, wherein the barrier layer is disposed outside the metallocene polyethylene layer.
Item 3.
Item 3. The packaging material according to Item 2, wherein the barrier layer is a transparent vapor-deposited polyethylene terephthalate layer.
Item 4.
Item 3. The packaging material according to Item 2, wherein the barrier layer is a metal foil layer.
Item 5.
Furthermore, the packaging material of said claim | item 4 which has a base material layer.
Item 6.
Item 6. The packaging material according to Item 5, wherein the base material layer is disposed outside the barrier layer.
Item 7.
Item 7. The packaging material according to Item 6, wherein the base material layer is a polyethylene terephthalate layer.
Item 8.
A packaging bag for packaging a powder composition containing cysteine protease, wherein the packaging material according to any one of items 1 to 7 is molded into a bag shape so that a sealant layer is an innermost layer.
Item 9.
Item 9. A package comprising the packaging bag according to Item 8 and a powder composition containing cysteine protease, wherein the powder composition is enclosed in the packaging bag.

  According to the packaging bag formed by molding the packaging material of the present invention into a bag shape, even when a powder composition containing cysteine protease is enclosed, the enzyme activity of the cysteine protease contained in the powder composition is reduced. Can be suppressed.

It is a figure which has a sealant layer and a metallocene polyethylene layer, and is a figure which shows the laminated structure laminated | stacked so that a sealant layer and a metallocene polyethylene layer may contact. It is a figure which has a sealant layer, a metallocene polyethylene layer, and a barrier layer, and is a figure which shows the laminated structure in which one or some arbitrary layers may intervene between the metallocene polyethylene layer and the barrier layer. It is a figure which shows a laminated structure which has a sealant layer, a metallocene polyethylene layer, and a barrier layer, and is laminated | stacked so that a metallocene polyethylene layer and a barrier layer may contact. It is a figure which shows the laminated structure which 1 or several arbitrary layers may interpose between a barrier layer and a base material layer. It is a figure which shows the laminated structure laminated | stacked so that a barrier layer and a base material layer may contact. It is a figure which shows the laminated structure of base material layer / metallocene polyethylene layer / barrier layer / metallocene polyethylene layer / sealant layer.

  Hereinafter, the present invention will be described in detail.

Packaging Material The packaging material of the present invention has a sealant layer, a metallocene polyethylene layer, and a barrier layer.

  The sealant layer is a layer that becomes the innermost layer when the packaging material of the present invention is molded into a bag shape, and is a layer intended to seal the contents by welding the sealant layers together by heat or the like. The sealant layer in the packaging material of the present invention is a polyethylene (PE) layer or an unstretched polypropylene (CPP) layer. Examples of the polyethylene layer include a low density polyethylene (LDPE) layer, a medium density polyethylene (MDPE) layer, a high density polyethylene (HDPE) layer, and a linear low density polyethylene (LLDPE) layer. Among these, A low density polyethylene layer and a linear low density polyethylene layer are preferable, and a linear low density polyethylene layer is more preferable.

  The thickness of the sealant layer is not particularly limited, but is usually about 1 to 200 μm, preferably about 2 to 100 μm, and more preferably about 10 to 70 μm.

  The metallocene polyethylene layer is also referred to as a super polyethylene (SPE) layer, and is an ethylene-α-olefin copolymer layer obtained by copolymerizing ethylene and α-olefin using a metallocene catalyst. . Examples thereof include a copolymer of ethylene and 1-butene, a copolymer of ethylene and 1-hexene, and a copolymer of ethylene and octene. Moreover, the metallocene polyethylene film made by any manufacturing method of the inflation molding method and the T-die molding method can also be used. Metallocene polyethylene is classified as linear low-density polyethylene (LLDPE), but it is greatly different in molecular weight distribution and comonomer distribution, so that it is obtained by copolymerization using a Ziegler-Natta catalyst. It is clearly distinguished from the shaped low density polyethylene (LLDPE). Also in the present invention, the metallocene polyethylene layer is clearly distinguished from the polyethylene layer in the sealant layer.

  The thickness of the metallocene polyethylene layer is not particularly limited, but is usually about 1 to 200 μm, preferably about 2 to 100 μm, more preferably about 5 to 50 μm.

  The barrier layer is a layer for the purpose of improving the strength of the packaging material and preventing moisture, oxygen, light and the like from entering the inside when formed into a bag shape. As the barrier layer, for example, a metal foil layer such as aluminum (AL) or stainless steel, a metal vapor deposited film layer obtained by vapor depositing a metal such as aluminum, a metal oxide such as alumina, or a silicon oxide is vapor deposited on the film. And a transparent vapor-deposited film layer, a polyvinylidene chloride layer, a barrier nylon layer, a polyvinylidene chloride-coated polyethylene terephthalate layer, a polyvinylidene chloride-coated biaxially stretched polypropylene layer, and the like. The film for depositing metal, metal oxide, silicate oxide or the like is not particularly limited, and examples thereof include polyethylene terephthalate (PET), unstretched polypropylene (CPP), biaxially stretched polypropylene (OPP), and polyethylene (PE). Is mentioned. Specific examples of the metal deposited film layer include a deposited polyethylene terephthalate (deposited PET) layer, a deposited unstretched polypropylene (deposited CPP) layer, a deposited biaxially stretched polypropylene (deposited OPP) layer, and a deposited polyethylene (deposited). PE) layer and the like, and specific examples of the transparent vapor-deposited film layer include a transparent vapor-deposited polyethylene terephthalate (transparent vapor-deposited PET) layer, a transparent vapor-deposited unstretched polypropylene (transparent vapor-deposited CPP) layer, etc. Is mentioned.

  The thickness of the barrier layer is not particularly limited as long as the above-described purpose of the barrier layer is achieved, but is usually about 1 to 100 μm, preferably about 2 to 70 μm, more preferably about 2 to 40 μm.

  In the packaging material of the present invention, the sealant layer and the metallocene polyethylene layer are laminated so as to contact each other. Here, in this specification, “the layers are laminated so that the (A layer and B layer) are in contact” means that the B layer is laminated on the A layer or the A layer is laminated on the B layer sequentially. This means that no arbitrary layer is interposed between the A layer and the B layer. That is, in the packaging material of the present invention, as shown in FIG. 1, the sealant layer and the metallocene polyethylene layer are laminated without any layers interposed between the sealant layer and the metallocene polyethylene layer.

  Moreover, in the packaging material of this invention, it is preferable that the barrier layer is arrange | positioned not the outer side of a sealant layer but the outer side of a metallocene polyethylene layer. Here, in this specification, “(A layer is arranged outside B layer)” means that the A layer is laminated on the B layer, and 1 between the A layer and the B layer. Or it means that a plurality of arbitrary layers may be interposed. That is, in the packaging material of the present invention, the barrier layer is preferably laminated on the metallocene polyethylene layer. In this case, as shown in FIG. 2, one or a plurality of arbitrary layers may be interposed between the barrier layer and the metallocene polyethylene layer, or as shown in FIG. And the metallocene polyethylene layer may be laminated so as to be in contact with each other. Among these, it is more preferable that the barrier layer and the metallocene polyethylene layer are laminated so as to contact each other. The use of the adhesive is determined according to the selection of any layer or manufacturing method that can be interposed between the barrier layer and the metallocene polyethylene layer, and the effect on the effect of the present invention with respect to the presence or absence of the use of the adhesive. There is no. Examples of the material that can be used in any layer that can be interposed between the barrier layer and the metallocene polyethylene layer include polypropylene, polyethylene terephthalate, ethylene-vinyl alcohol copolymer, nylon polyvinyl alcohol, and ethylene-vinyl acetate. A copolymer, polyacrylonitrile, polystyrene, cellophane, paper, etc. are mentioned.

  In the present specification, the laminated structure of the packaging material may be expressed using “/” such as “A layer / B layer / C layer”. In this expression, the innermost layer when the packaging material is molded into a bag shape is described on the rightmost side, and approaches the outermost layer as it goes to the left. For example, as described above, when “A layer / B layer / C layer” is described, the A layer is the outermost layer, the C layer is the innermost layer, and the B layer is between the A layer and the C layer. Indicates an intervening layer.

  That is, the packaging material of the present invention may have a laminated structure of barrier layer / 1 or a plurality of arbitrary layers / metallocene polyethylene layer / sealant layer, or a laminated structure of barrier layer / metallocene polyethylene layer / sealant layer. In these, the laminated structure which is a barrier layer / metallocene polyethylene layer / sealant layer is more preferable, and as such a laminated structure, transparent vapor deposition PET / SPE / CPP, AL / SPE / PE, etc. are mentioned, for example.

  Further, when the barrier layer in the packaging material of the present invention is a metal foil layer, the packaging material of the present invention further has a single layer or a base layer composed of a plurality of layers on the outer layer side of the barrier layer. Also good.

  Although it does not restrict | limit especially as a base material layer, For example, a biaxially stretched polyethylene (OPP), stretched nylon (ONY), paper, a polyethylene terephthalate (PET) layer etc. can be illustrated. Among these, a polyethylene terephthalate (PET) layer is preferable.

  The thickness of the base material layer is not particularly limited, but is usually about 10 to 100 μm, preferably about 10 to 60 μm.

  When the packaging material of this invention has a base material layer, it is preferable that the base material layer is laminated | stacked on the outer side of an above described barrier layer. In this case, as shown in FIG. 4, one or a plurality of arbitrary layers may be interposed between the base material layer and the barrier layer, or as shown in FIG. It may be laminated so as to be in contact with the barrier layer. Optional layers that can be interposed between the base material layer and the barrier layer include a low density polyethylene layer, a medium density polyethylene layer, a high density polyethylene layer, a linear low density polyethylene layer, a metallocene polyethylene layer, a polypropylene layer, ethylene Examples thereof include a vinyl alcohol copolymer layer, a nylon polyvinyl alcohol layer, an ethylene-vinyl acetate copolymer layer, a polyacrylonitrile layer, a polystyrene layer, and a cellophane layer.

  Among these, a laminated structure of base material layer / metallocene polyethylene layer / barrier layer / metallocene polyethylene layer / sealant layer shown in FIG. 6 is preferable, and as such a laminated structure, for example, PET / SPE / AL / SPE / PE and the like.

  The method for forming the above laminated structure in the packaging material of the present invention, in other words, the production method of the packaging material of the present invention is not particularly limited, and examples thereof include known methods such as an extrusion lamination method and a polysand lamination method. It is done.

  The packaging material of the present invention is used for packaging a powder composition containing cysteine protease. That is, the packaging material of the present invention is a packaging material for packaging a powder composition containing cysteine protease. More specifically, the packaging material of the present invention is molded into a bag shape as described later and used as a packaging bag. By enclosing a powder composition containing cysteine protease in a packaging bag obtained by molding the packaging material of the present invention into a bag shape, a decrease in enzyme activity of cysteine protease contained in the powder composition can be suppressed.

  The packaging bag of the present invention is obtained by molding the above-described packaging material of the present invention into a bag shape so that the sealant layer is the innermost layer, that is, the sealant layer is a layer in contact with the contents. It is done. The method for molding into a bag shape is not particularly limited. For example, in the case of molding a rectangular packaging bag, the two packaging materials of the present invention are overlapped so that the sealant layers are in contact with each other, and three sides are formed. On the other hand, it can be molded into a bag shape having an opening by performing heat sealing. Then, the contents can be enclosed in a packaging bag by putting the contents and performing heat sealing.

  The powder composition which is the content enclosed in the packaging bag of this invention will not be restrict | limited especially if it is a composition which contains cysteine protease and is a powder form. Examples of the cysteine protease include papain family cysteine protease, ginger protease, orizain, and cathepsins. In addition, the said powder composition may contain the above-mentioned cysteine protease individually by 1 type, and may contain it in combination of 2 or more types.

  Moreover, the said powder composition may contain other components other than cysteine protease. Examples of other components include bases (sugar alcohols such as reduced palatinose, palatinose, and maltitol), thickening polysaccharides (eg, xanthan gum, guar gum, hyaluronic acid, hydroxyethyl cellulose, etc.), pH adjusters, and coloring agents. , Flavoring agents, fragrances, moisture inhibitors and the like.

  Examples of the powder composition containing cysteine protease are described in Japanese Patent Application No. 2015-090703 and Japanese Patent Application No. 2015-106416 (both unpublished) for which the present inventors have applied for a patent. Examples thereof include a composition. Examples include powder compositions containing cysteine proteases such as papain, reduced palatinose, xanthan gum, moisture inhibitors, colorants, pH adjusters and the like.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated further in detail, this invention is not limited to the following example. In the following, papain was used as a representative example of cysteine protease.

  Various components shown in Table 1 below were mixed at the ratio shown in Table 1 to prepare a powder composition.

  Subsequently, sample No. having the configuration shown in Table 2 below. The packaging material of 1-11 was shape | molded in the bag shape of length 60mm x width 70mm, and the packaging bag was produced. Sample No. 11 is a sample No. 11 packaging bag. In one packaging bag, the innermost layer and the outermost layer are produced so that they are turned inside out.

  Sample No. All of the packaging materials 1 to 11 are commercially available products, and the trade names are as follows.

・ Sample No. 1 and 11: Rami Zip AL (trade name, manufactured by Nihon Sha)
・ Sample No. 2: Lami Zip AL (trade name, manufactured by Nihon Sha)
・ Sample No. 3: Unipack (trade name, manufactured by Nippon Shokubai)
・ Sample No. 4: Lami grip VP (trade name, manufactured by Nippon Shokubai Co., Ltd.)
・ Sample No. 5: Lami Grip OP (trade name, manufactured by Nihon Sha)
・ Sample No. 6: Lami Zip LZ (trade name, manufactured by Nihon Sha)
・ Sample No. 7: Rami Zip BY (trade name, manufactured by Nippon Shokubai Co., Ltd.)
・ Sample No. 8: Unipack (trade name, manufactured by Japan Corporation)
・ Sample No. 9: Hygroscopic storage bag (trade name, manufactured by ASONE)
・ Sample No. 10: Kakukei CPP bag (trade name, manufactured by Kakkei)

  In the “Packaging Bag Configuration” column in Table 2 below, the laminated structure of the packaging material and the thickness of each layer are shown. For example, a sample No. indicated as “PET12 / SPE15 / AL7 / SPE15 / PE40”. 1 indicates that the innermost layer is a PE layer and the outermost layer is a PET layer. Further, the number given to the right of the symbol indicating each layer indicates the thickness (unit: μm) of each layer, and “PE40” indicates that the PE layer is 40 μm.

  Next, 2 g of the powder composition prepared above was added to each of sample Nos. After putting in the packaging bag of 1-11 and sealing, the packaging bag was shaken up and down 10 times. Thereafter, the enzyme activity of papain was measured in accordance with the “Enzyme Activity Measurement Method” described in “Papain” (pages 523 to 524) of the 8th edition “Ingredient Standard / Preservation Standard” of the Food Additives. Specifically, the enzyme activity of papain was measured as follows.

  8.75 g of L-cysteine hydrochloride was dissolved in about 800 ml of water, dissolved by adding 2.23 g of disodium ethylenediaminetetraacetate, adjusted to pH 4.5 with 1 mol / L sodium hydroxide solution, and water was added. The dilution was adjusted to 1000 ml. Next, sample no. From 0.5 to 11 packaging bags, 0.500 g of the powder composition was accurately weighed, and the diluent prepared above was added and dissolved to prepare 50 ml of sample solutions.

5 ml of casein test solution (pH 8.0) was accurately weighed and placed in a test tube, heated at about 37 ° C. for 10 minutes, then added with 1 ml of the sample solution prepared above, and immediately shaken to obtain a mixed solution. Next, after reacting the mixed solution at about 37 ° C. for 10 minutes, 5 ml of a trichloroacetic acid test solution was added and shaken, allowed to stand at about 37 ° C. for 30 minutes, and filtered using filter paper for quantitative analysis (5 types C). . For the filtrate except the first 3 ml, the absorbance (A _T ) at a wavelength of 275 nm was measured using water as a control. Separately, add 5 ml of the trichloroacetic acid test solution to 1 ml of the sample solution and shake well, then add 5 ml of casein test solution (pH 8.0), shake well, and leave at 37 ° C. for 30 minutes. Then, filtration and absorbance (A _b ) were measured. Further, by using a 0.1 mol / L hydrochloric acid, the produced tyrosine standard solution containing tyrosine 50.0μg in 1 ml, as control of water, was measured the absorbance at a wavelength 275nm to _{(A S).} Further, with respect to 0.1 mol / L hydrochloric acid, the absorbance (A _S0 ) at a wavelength of 275 nm was measured using water as a control.

  From each absorbance measured above, the enzyme activity (unit / g) was measured by the following formula (1).

  Using the enzyme activity value of the powder composition calculated in the same manner as described above before putting in the packaging bag as a reference (100%), the residual rate of enzyme activity was calculated from each enzyme activity value calculated above. As for the remaining rate of enzyme activity obtained, the enzyme activity remaining rate of 95% or more was “excellent (◯)”, and the enzyme activity remaining rate was 90% or more and less than 95% “possible ( Evaluations were made based on the criteria of “impossible (×)” for those that were less than 90%. The remaining rate of enzyme activity and the evaluation results are shown in the “residual rate” column and the “evaluation result” column of Table 2 below.

  As is apparent from Table 2 above, sample no. When the packaging materials 1, 2, and 4 were used, it was confirmed that the residual rate of enzyme activity was as high as 95% or more. Sample No. 1, 2 and 4 and sample no. From the comparison with 3 and 5-11, it has a sealant layer, a metallocene polyethylene layer, and a barrier layer, the innermost layer is a PE layer or a CPP layer, and the innermost layer and the SPE layer are laminated in contact with each other. It was found that the decrease in the enzyme activity of cysteine protease contained in the powder composition can be suppressed only when the packaging material having the laminated structure is used.

Claims (9)

A packaging material for packaging a powder composition containing cysteine protease,
The packaging material has a sealant layer, a metallocene polyethylene layer, and a barrier layer, and is laminated so that the sealant layer and the metallocene polyethylene layer are in contact with each other,
The sealant layer is a polyethylene layer or an unstretched polypropylene layer,
Packaging material. The packaging material according to claim 1, wherein the barrier layer is disposed outside the metallocene polyethylene layer. The packaging material according to claim 2, wherein the barrier layer is a transparent vapor-deposited polyethylene terephthalate layer. The packaging material according to claim 2, wherein the barrier layer is a metal foil layer. Furthermore, the packaging material of Claim 4 which has a base material layer. The packaging material according to claim 5, wherein the base material layer is disposed outside the barrier layer. The packaging material according to claim 6, wherein the base material layer is a polyethylene terephthalate layer. A packaging bag for packaging a powder composition containing cysteine protease, wherein the packaging material according to any one of claims 1 to 7 is molded into a bag shape so that a sealant layer is an innermost layer. A package comprising the packaging bag according to claim 8 and a powder composition containing cysteine protease, wherein the powder composition is enclosed in the packaging bag.

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