JP2017015488A - Imaging device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an imaging device that can highly accurately acquire information on a tissue of a biology.SOLUTION: An imaging device (1) comprises: a sample support part (2) that supports a biological tissue (BT); a detection unit (21) that detects light radiated from the tissue by irradiation of infrared light; a calibration reference part (5) that is used in calibration of the detection unit; and a control unit (7) that moves at least one of the calibration reference part and the sample support unit, and switches an arrangement state where the calibration reference part is arranged in a detection range (AI) of the detection unit and a retreat state where the calibration reference unit is arranged out of the detection range of the detection unit.SELECTED DRAWING: Figure 1

Description

  The present invention relates to an imaging apparatus.

  In the field of medical treatment or the like, a technique has been proposed in which a biological tissue is imaged and the image is used for various diagnoses, examinations, observations, and the like (for example, see Patent Document 1 below). The apparatus according to Patent Document 1 is a technique that, for example, irradiates a body tissue with infrared rays and acquires an image of a subcutaneous blood vessel based on the infrared rays reflected by the body tissues.

JP 2006-102360 A

  For example, in the imaging technique as described above, it is desired that information on a biological tissue can be acquired with high accuracy.

  According to an aspect of the present invention, a specimen support unit that supports biological tissue, a detection unit that detects light emitted from the tissue by irradiation with infrared light, a calibration reference unit that is used for calibration of the detection unit, An arrangement state in which at least one of the calibration reference unit and the sample support unit is moved, the calibration reference unit is arranged within the detection range of the detection unit, and a retracted state in which the calibration reference unit is arranged outside the detection range of the detection unit And an imaging device including a control unit that switches between.

It is a figure which shows the imaging device which concerns on embodiment. It is a figure which shows the illumination unit of the imaging device which concerns on embodiment. It is a figure which shows the imaging unit of the imaging device which concerns on embodiment. It is a figure which shows the calibration reference | standard part and switching part of the imaging device which concern on embodiment. It is a figure which shows the accommodating part of the imaging device which concerns on embodiment. It is a figure which shows the exhaust port of the imaging device which concerns on embodiment. It is a flowchart which shows the imaging method which concerns on embodiment. It is a figure which shows the other form of the switching part which concerns on embodiment. It is a figure which shows the other form of the switching part which concerns on embodiment.

  Embodiments will be described. FIG. 1A is a diagram illustrating an appearance of the imaging device 1 according to the embodiment, and FIG. 1B is a diagram illustrating a configuration of the imaging device 1. In the XYZ orthogonal coordinate system in the figure, the X direction and the Y direction are, for example, the horizontal direction, and the Z direction is, for example, the vertical direction. In each of the X direction, Y direction, and Z direction, the direction of the arrow is appropriately referred to as a + side (eg, + X side), and the opposite side is referred to as a − side (eg, −X side).

  The imaging device 1 is used for medical support such as pathological diagnosis support, clinical diagnosis support, and observation support, for example. As shown in FIG. 1B, the imaging device 1 includes a specimen support unit 2, an illumination unit (illumination unit) 3, a detection unit (imaging unit) 4, a calibration reference unit 5, a switching unit 6, The control part 7 and the accommodating part 8 are provided. The specimen support unit 2 supports a specimen including a biological tissue BT. The sample support part 2 is a rectangular plate-shaped member, for example. For example, the upper surface (mounting surface) of the specimen support unit 2 is disposed substantially parallel to the horizontal direction, and the tissue BT can be mounted on the upper surface (mounting surface).

  The tissue BT is, for example, a human tissue, but may be a tissue of a non-human organism (eg, an animal). The tissue BT may be a tissue cut from a living organism or may be a tissue associated with a living organism. The tissue BT may be a living organism (living body) tissue (living tissue) or a living organism (dead body) after death. The tissue BT may be an object extracted from a living organism. The tissue BT may include any organ of an organism, may include skin, and may include internal organs inside the skin. In addition, the tissue BT may contain one or both of secretion and excretion. Further, the tissue BT may be a material in which a substance that receives light and emits light by excitation (eg, a fluorescent substance or a phosphorescent substance) is added to a living tissue. The tissue BT may be fixed using a tissue fixing solution such as formalin.

  For example, the illumination unit 3 is disposed above the sample support 2 and includes infrared light (hereinafter referred to as “infrared light” in this specification, including “near infrared light”). To the tissue BT. The illumination unit 3 is attached to the imaging unit 4, for example. The illumination unit 3 includes a light source unit 11, a holding member 12, a visible light source unit 13, and a light source moving unit 14. The light source unit 11 emits infrared light. The holding member 12 holds the light source unit 11. The holding member 12 is, for example, a plate-like member, and holds the light source unit 11 on the lower surface side. The light source moving unit 14 changes the irradiation angle of the infrared light with respect to the tissue BT. In the present embodiment, the imaging device 1 includes a diffusing member 15. The diffusion member 15 diffuses infrared light from the light source unit 11. Infrared light from the light source unit 11 is diffused by the diffusion member 15 and then irradiated to the tissue BT. The illumination unit 3 can irradiate the tissue BT with near-infrared light. The illumination unit 3 can irradiate the tissue BT with a single narrow wavelength band of infrared light. Further, the illumination unit 3 may be capable of performing shadowless illumination such as a shadowless lamp.

  In the present embodiment, the illumination unit 3 can also irradiate the tissue BT with visible light. The visible light source unit 13 emits visible light. The visible light source unit 13 is held by the holding member 12. The holding member 12 holds the visible light source unit 13 on the lower surface side, for example. The light source moving unit 14 can also change the irradiation angle (eg, irradiation direction) of visible light with respect to the tissue BT. Visible light from the visible light source unit 13 is diffused by the diffusion member 15 and then irradiated to the tissue BT.

  FIG. 2A is a diagram showing the illumination unit 3 with the diffusion member 15 attached, and FIG. 2B is a diagram showing the illumination unit (A) with the diffusion member 15 removed. is there. FIG. 2C is a plan view of the illumination unit 3. FIG. 2D illustrates the light source moving unit 14. As shown in FIGS. 2A and 2B, the diffusion member 15 is provided so as to cover the emission side of the illumination unit 3. The diffusing member 15 has an opening 15a, and an optical path (an optical axis 21a of the first imaging unit 21 described below and its surroundings) between the imaging unit 4 and the sample support unit 2 is disposed inside the opening 15a. . For example, the diffusing member 15 is arranged on the emission side of the illumination unit 3 and is integrally provided with the light source unit 11 and the visible light source unit 13, and includes the specimen support unit 2, the light source unit 11, and the visible light source unit 13. Arranged between. For example, the diffusing member 15 is disposed on the light receiving side of the imaging unit 4 and has an opening (for example, an opening 15a) through which light such as infrared light or visible light (light through the tissue BT) passes. As described above, the diffusing member 15 uses the opening, and the light emission side of the light source unit 11 among the light source unit 11, the visible light source unit 13, and the imaging unit (the first imaging unit 21 and / or the second imaging unit 22). And it is provided so that the light emission side of the visible light source part 13 may be covered. Further, the diffusion member 15 constitutes an upper portion (ceiling portion) with respect to the specimen support portion 2 in the retracted state of the calibration reference portion 5.

  As shown in FIG. 2C, a plurality of illumination units 3 are arranged around an optical axis (eg, an optical axis of light received by a light receiving element) 21a of an imaging unit (detection unit). In each lighting unit 3, the light source unit 11 includes a plurality of light sources 16. For example, each of the plurality of light sources 16 is a light emitting diode (LED), but may include a solid light source such as a laser diode (LD) or a lamp light source such as a halogen lamp. The plurality of light sources 16 emit infrared light having different wavelength bands. The wavelength band of the infrared light emitted from each of the plurality of light sources 16 is selected from, for example, a wavelength band of about 700 nm or more and about 2500 nm or less. The wavelength bands of infrared light emitted from each of the plurality of light sources 16 are set so as not to overlap each other, for example, but may be overlapped, and two or more light sources 16 emit infrared light in the same wavelength band. May be. In each lighting unit 3, the number of light sources 16 included in the light source unit 11 is six in FIG. 2C, but may be one or any number of two or more. In each lighting unit 3, the plurality of light sources 16 are all held by the holding member 12, but the plurality of light sources 16 may be divided into a plurality of members and held. Further, for example, the plurality of light sources 16 are controlled by the control unit 7 and emit infrared light selectively or collectively.

  The visible light source unit 13 includes a light source such as a light emitting diode (LED). This light source may be a solid light source such as a laser diode (LD) or a lamp light source such as a halogen lamp. The visible light source unit 13 emits visible light in at least a part of a wavelength band of, for example, about 380 nm to about 750 nm. The visible light source part 13 is provided in each illumination unit 3, for example. In each lighting unit 3, the visible light source unit 13 is held by, for example, the same holding member 12 as the plurality of light sources 16 of the light source unit 11, but may be held by a member different from the holding member 12. The number of light sources of the visible light source unit 13 provided in each illumination unit 3 is one in FIG. 2C, but may be two or more. When the visible light source unit 13 includes a plurality of light sources, the wavelength bands of visible light emitted from the plurality of light sources may be different from each other for two or more light sources, or may be the same for two or more light sources.

  As shown in FIG. 2D, the light source moving unit 14 changes the irradiation angle of the infrared light IR to the tissue BT (eg, the irradiation direction of the light source unit 11 and the emission direction). The irradiation direction D1 of the light source unit 11 is, for example, the direction of the central axis of the infrared light IR (beam) emitted from the light source unit 11. The light source moving unit 14 changes the irradiation angle of the infrared light IR, for example, by changing the posture of the holding member 12 (for example, the angle with the optical axis 21a of the first imaging unit 21). The irradiation angle of the infrared light IR from the light source unit 11 is set so that, for example, the positional relationship between the light source unit 11 and the first imaging unit 21 deviates from the regular reflection relationship regarding the surface of the tissue BT. The irradiation angle of the infrared light IR from the light source unit 11 may be set so that the positional relationship between the light source unit 11 and the first imaging unit 21 deviates from the regular reflection relationship with respect to the upper surface of the sample support unit 2.

  For example, the light source moving unit 14 connects the holding member 12 and the imaging unit 4 and moves (eg, rotates) the holding member 12 with respect to the imaging unit 4. Thereby, the attitude | position of the holding member 12 changes and the irradiation angle of the infrared light IR from the light source part 11 changes (the irradiation direction D1 after a change is shown with a dashed-two dotted line). The light source moving unit 14 includes, for example, a gear and transmits a driving force for moving the holding member 12. The light source moving unit 14 may include an actuator such as an electric motor that supplies a driving force for moving the holding member 12, or may not include an actuator. When the light source moving unit 14 includes an actuator, the actuator may be controlled by the control unit 7. The control unit 7 may control the irradiation angle of the infrared light IR by controlling the light source moving unit 14. Further, when the light source moving unit 14 does not include an actuator, for example, the light source moving unit 14 may be driven by an operator (user) human power. In addition, the holding member 12 may be connected (eg, supported) to an object different from the imaging unit 4 or may not be connected (eg, supported) to the imaging unit 4. The light source moving unit 14 may change the irradiation angle of the infrared light for each lighting unit 3, or change the irradiation angle of the infrared light at a time in two or more lighting units 3 by a link mechanism, for example. You may let them.

  The plurality of lighting units 3 have the same configuration, but two or more of them may have different configurations. For example, in one illumination unit 3, at least one of the positional relationship of the plurality of light sources 16 with respect to the holding member 12, the number of the plurality of light sources 16, and the wavelength band of infrared light emitted from the plurality of light sources 16 is different from the other illumination units. It may be different from the unit 3. Further, the imaging device 1 may not include at least a part of the illumination unit 3. For example, the illumination unit 3 may be attached to the imaging device 1 so as to be replaceable, and may be attached when the imaging device 1 performs imaging. In addition, at least a part of the lighting unit 3 may be a part of equipment (for example, a room light) in which the imaging device 1 is used.

  Returning to the description of FIG. 1, the imaging unit 4 as a detection unit includes a first imaging unit 21 and a second imaging unit 22 as detection units. The first imaging unit 21 is, for example, an infrared camera, and images the tissue BT by irradiation with infrared light. The first imaging unit 21 detects light (eg, reflected light, scattered light, transmitted light, reflected scattered light, etc.) emitted from the tissue BT by irradiation with infrared light. The first imaging unit 21 includes an imaging optical system (detection optical system) 23 and an imaging element (light receiving element) 24. The imaging optical system 23 has an AF mechanism (autofocus mechanism), for example, and forms an image of the tissue BT. The optical axis 21 a of the first imaging unit 21 is coaxial with the optical axis of the imaging optical system 23.

  The image sensor 24 captures an image formed by the imaging optical system 23. The imaging device 24 includes a two-dimensional image sensor such as a CCD image sensor or a CMOS image sensor. The image sensor 24 has, for example, a structure in which a plurality of pixels arranged two-dimensionally and a photodetector such as a photodiode is disposed in each pixel. The imaging device 24 uses, for example, InGaAs (indium gallium arsenide) as a photodetector, and has sensitivity in the wavelength band of infrared light emitted from the light source unit 11. The detection range A1 of the first imaging unit 21 is, for example, an imaging region that can be imaged by the first imaging unit 21 on the sample support unit 2 and a visual field region of the first imaging unit 21 on the sample support unit 2. The imaging region of the first imaging unit 21 is, for example, a region that is optically conjugate with the light receiving region (the region where the photodetector is disposed) of the imaging element 24. The field area of the first imaging unit 21 is, for example, an area optically conjugate with the inside of the field stop of the imaging optical system 23. For example, the first imaging unit 21 generates captured image data as an imaging result (detection result). For example, the first imaging unit 21 supplies captured image data to the control unit 7.

  The second imaging unit 22 is, for example, a visible camera, and images the tissue BT by irradiation with visible light. For example, the second imaging unit 22 detects light reflected and scattered from the surface of the tissue BT in the visible light from the visible light source unit 13. The second imaging unit 22 includes an imaging optical system (not shown) and an imaging element (not shown). The imaging optical system has an AF mechanism (autofocus mechanism), for example, and forms an image of the tissue BT. The imaging element captures an image formed by the imaging optical system. The imaging element includes a two-dimensional image sensor such as a CCD image sensor or a CMOS image sensor. The image sensor 24 has, for example, a structure in which a plurality of pixels arranged two-dimensionally and a photodetector such as a photodiode is disposed in each pixel. The imaging element uses, for example, Si (silicon) as a photodetector, and has sensitivity in the wavelength band of visible light emitted from the visible light source unit 13. For example, the second imaging unit 22 generates captured image data as an imaging result (detection result). For example, the second imaging unit 22 supplies captured image data to the control unit 7.

  In the present embodiment, the imaging device 1 includes a size changing unit 31. The size changing unit 31 relatively moves the first imaging unit 21 and the sample support unit 2 in the direction of the optical axis 21a of the first imaging unit 21, and changes the size of the detection range A1. For example, the size changing unit 31 moves the imaging unit 4 provided with the first imaging unit 21 to move the first imaging unit 21 and the sample support unit 2 of the imaging unit (for example, the first imaging unit 21). Relative movement is made in the optical axis direction (eg, the optical axis direction of light received by the detection element). In the present embodiment, the illumination unit 3 having the diffusion member 15 is connected to the imaging unit 4, and the size changing unit 31 moves the illumination unit 3 together with the imaging unit 4. The size changing unit 31 includes, for example, an actuator (not shown) such as an electric motor, and a transmission unit (not shown) that transmits a driving force from the actuator to the imaging unit 4. For example, the size changing unit 31 is controlled by the control unit 7 and moves the imaging unit 4 by the movement amount specified by the control unit 7.

  Note that the imaging device 1 may not include the second imaging unit 22. Further, the second imaging unit 22 may be included in a device external to the imaging device 1. Further, the size changing unit 31 may move the specimen support unit 2 forward and backward with respect to the first imaging unit 21. In this case, the size changing unit 31 may move or move the imaging unit 4. It does not have to be. Further, the imaging device 1 may not include the size changing unit 31. The imaging device 1 (eg, the imaging optical system 23) may include a zoom mechanism (eg, a zoom lens).

  3A and 3B are diagrams illustrating movement of the imaging unit 4 of the imaging apparatus 1 according to the embodiment. In FIG. 3B, the imaging unit 4 is disposed below (eg, the vertical direction approaching the object) as compared to FIG. As the imaging unit 4 moves downward (eg, in a vertical direction approaching the object), the imaging unit 4 approaches the opposite object (eg, sample support unit 2), and the detection range A1 on this object (see FIG. 1B). Becomes narrower. For example, the imaging unit 4 can acquire a captured image in which an object in the detection range A1 is enlarged as the detection range A1 becomes narrower. Further, as the imaging unit 4 moves upward (eg, in the vertical direction away from the object), the imaging unit 4 moves away from the opposite object (eg, the sample support unit 2), and the detection range A1 on this object (see FIG. 1B). ) Becomes wider. For example, the imaging unit 4 can acquire a captured image obtained by reducing an object in the detection range A1 as the detection range A1 becomes wider.

  Next, the calibration reference unit 5 and the switching unit 6 will be described with reference to FIGS. 1 and 4. FIG. 4 is a diagram illustrating the calibration reference unit 5 and the switching unit 6. The calibration reference unit 5 has, for example, at least a calibration portion (eg, standard white plate, standard gray plate, standard black plate, etc.) serving as a measurement reference on the surface, and calibration of the first imaging unit 21 (eg, imaging). Used to calibrate the brightness of light received by the light receiving element of the light. The calibration reference unit 5 is calibrated and verified by a public organization such as a standard white plate. The calibration reference portion 5 may be a plate-shaped member, a block-shaped (bulk-shaped) member, a sheet-shaped member, or another shape member. The calibration reference unit 5 has a substantially flat reflectance in a predetermined wavelength band (eg, 300 nm to 2500 nm). The calibration reference unit 5 can also be used for calibration of the second imaging unit 22. When calibrating the first imaging unit 21, the control unit 7 arranges the calibration reference unit 5 within the detection range A <b> 1 of the first imaging unit 21 (in an arrangement state).

  As shown in FIG. 1, when the tissue BT is imaged by the first imaging unit 21, the control unit 7 places the calibration reference unit 5 outside the detection range A1 of the first imaging unit 21 (calibration reference). The unit 5 is in the retracted state). The control unit 7 moves at least one of the calibration reference unit 5 and the sample support unit 2 to switch between the retracted state (FIG. 1) and the arrangement state (FIG. 4) of the calibration reference unit 5. For example, when the control unit 7 switches the calibration reference unit 5 from the retracted state (FIG. 1) to the arranged state (FIG. 4), the sample supporting unit 2 is arranged outside the detection range A <b> 1 of the first imaging unit 21. The switching unit 6 is controlled by the control unit 7 and relatively moves the calibration reference unit 5 and the sample support unit 2. The control unit 7 controls the switching unit 6 to switch between the retracted state and the arrangement state of the calibration reference unit 5.

  First, an operation when the calibration reference unit 5 is switched from the retracted state (FIG. 1) to the arranged state (FIG. 4) will be described. In the retracted state of the calibration reference unit 5 (FIG. 1), the sample support unit 2 and the calibration reference unit 5 are disposed along the direction of the optical axis 21a of the first imaging unit 21, for example. Further, for example, the calibration reference unit 5 is located below the sample support unit 2 (Z direction in FIG. 4) in the retracted state, and is arranged and accommodated so that one surface of the calibration reference unit 5 is covered by the sample support unit 2. Stored in the unit 8. For example, the calibration reference unit 5 is arranged on the opposite side of the detection unit (imaging unit 4) with respect to at least a part of the sample support unit 2 in the retracted state. For example, in the retracted state, the calibration reference portion 5 and the sample support portion 2 are disposed so as to face each other. For example, in the retracted state, the surface of the calibration reference portion 5 on which the white portion for calibration is provided is disposed so as to face the placement surface of the specimen support portion 2 or the surface opposite to the placement surface. For example, the standard support unit 2 is disposed above the calibration reference unit 5 in the retracted state, and is disposed at a position where light (eg, infrared light, visible light) incident on the calibration reference unit 5 can be blocked. . The control unit 7 rotates at least one of the sample support unit 2 and the calibration reference unit 5 around an axis that is not parallel to the direction of the optical axis 21 a of the first imaging unit 21. Further, the control unit 7 rotates at least one of the sample support unit 2 and the calibration reference unit 5 around an axis perpendicular to or perpendicular to the direction of the optical axis 21 a of the first imaging unit 21. For example, the calibration reference unit 5 is held on the side opposite to the first imaging unit 21 with respect to the specimen support unit 2 in the retracted state (FIG. 1). For example, at least a part of the calibration reference unit 5 (eg, the surface or one surface of the calibration reference unit 5) is covered with the sample support unit 2 in the retracted state. In the retracted state, the surface of the calibration reference portion 5 on which the calibration portion is formed is covered with the sample support portion 2. In the retracted state of the calibration reference unit 5, the sample support unit 2 is disposed, for example, at a position that blocks the optical path between the calibration reference unit 5 and the first imaging unit 21.

  The control unit 7 rotates the sample support unit 2 when switching to the arrangement state (FIG. 4) of the calibration reference unit 5. The sample support portion 2 is, for example, a rectangular plate shape, and one end portion (−Y side end portion) of a side (eg, short side) parallel to the Y direction is supported by the rotation shaft 32. The rotation shaft 32 is, for example, parallel to the X direction (eg, parallel to the long side of the sample support unit 2) and is rotatable around the X direction. The switching unit 6 includes, for example, the rotating shaft 32, the actuator 36 that supplies driving force to the rotating shaft 32, and a transmission unit (not shown) that transmits the driving force from the actuator 36 to the rotating shaft 32.

  When the control unit 7 switches to the arrangement state of FIG. 4, the control unit 7 controls the actuator 36 of the switching unit 6, and moves the rotation shaft 32 in the direction away from the calibration reference unit 5 (counterclockwise in FIG. 4). Rotate around the center. The calibration reference unit 5 is disposed within the detection range A1 of the first imaging unit 21 as the sample support unit 2 retracts from the optical path between the calibration reference unit 5 and the first imaging unit 21.

  For example, the control unit 7 may adjust the interval H1 between the calibration reference unit 5 and the first imaging unit 21 when switching to the arrangement state. For example, the control unit 7 controls the size changing unit 31 to set the interval H1 between the calibration reference unit 5 and the first imaging unit 21 in the arrangement state, and the sample support unit 2 and the first imaging in the retracted state (see FIG. 1). Adjustment is made to be substantially the same as the distance H2 from the portion 21. In addition, for example, the calibration reference unit 5 is arranged in the same plane as the sample support unit 2 or arranged in parallel to the sample support unit 2 in the arrangement state (or retracted state).

  For example, the control unit 7 controls the switching unit 6 and the size changing unit 31 so that the first imaging unit 21 and the sample support unit 2 do not collide. For example, while the switching unit 6 moves the sample support unit 2 and the calibration reference unit 5 relative to each other, the control unit 7 captures the imaging unit 4 (eg, the first imaging unit 21) and the sample support unit by the size changing unit 31. Restrict or prohibit relative movement with 2 For example, the control unit 7 determines whether or not the switching unit 6 is operating based on a control signal that controls the switching unit 6. When it is determined that the switching unit 6 is operating, the control unit 7 does not need to supply an operation start control signal to the size changing unit 31, and supplies the operation start control signal for a certain period (example). , Until the operation of the imaging unit 4 ends). For example, when the control unit 7 switches to the arrangement state of the calibration reference unit 5, after the relative movement (rotation) between the sample support unit 2 and the calibration reference unit by the switching unit 6 is completed, the imaging unit by the size changing unit 31 is used. The relative movement between 4 and the specimen support 2 is started.

  Note that the control unit 7 may determine whether or not the switching unit 6 is operating based on the position information of the sample support unit 2, for example. For example, the imaging apparatus 1 includes a position sensor that detects the position of the sample support unit 2, and the control unit 7 determines whether the sample support unit 2 is operating based on the detection result (position information) of the position sensor. It may be determined. This position sensor may be an encoder provided in the switching unit 6, for example. For example, when the operation of the size changing unit 31 is prohibited or restricted, the control unit 7 may notify the user to that effect by blinking a lamp, sound, or the like.

  Next, the operation when switching the calibration reference unit 5 from the arrangement state (FIG. 4) to the retracted state (FIG. 1) will be described. The control unit 7 controls the actuator 36 of the switching unit 6 to switch the sample support unit 2 closer to the calibration reference unit 5 when switching from the arrangement state (FIG. 4) of the calibration reference unit 5 to the retracted state (FIG. 1). Rotate (clockwise in FIG. 4). For example, the calibration reference unit 5 is disposed outside the detection range A1 of the first imaging unit 21 by blocking the optical path between the calibration reference unit 5 and the first imaging unit 21 by the sample support unit 2.

  For example, the sample support 2 is supported by the stopper 35 at the other end (+ Y end) of a side parallel to the Y direction (for example, the short side), and is rotated clockwise when viewed from the + X side. Is regulated. For example, the stopper 35 regulates the rotational position of the sample support unit 2 so that the sample support unit 2 and the calibration reference unit 5 do not contact (collision). For example, the calibration reference unit 5 is arranged in non-contact with the sample support unit 2 in the retracted state.

  For example, the control unit 7 may adjust the interval between the calibration reference unit 5 and the first imaging unit 21 when switching from the arrangement state of the calibration reference unit 5 to the retracted state. For example, the control unit 7 controls the switching unit 6 and the size changing unit 31 so that the first imaging unit 21 and the sample support unit 2 do not collide. For example, while the size changing unit 31 moves the imaging unit 4 (for example, the first imaging unit 21) and the calibration reference unit 5 relative to each other, the control unit 7 controls the sample support unit 2 and the calibration reference unit 5 by the switching unit 6. Restrict or prohibit relative movement of. For example, the control unit 7 determines whether or not the size changing unit 31 is operating based on a control signal for controlling the size changing unit 31. If the control unit 7 determines that the size changing unit 31 is operating, the control unit 7 may not supply an operation start control signal to the switching unit 6, and may supply the operation start control signal to the size changing unit 31. It may be delayed until the end of the operation. For example, when the control unit 7 switches the calibration reference unit 5 to the retracted state, after the relative movement between the imaging unit 4 and the calibration reference unit 5 by the size changing unit 31 is completed, Start relative movement with the calibration reference section.

  Note that the control unit 7 may determine whether or not the size changing unit 31 is operating based on the position information of the first imaging unit 21, for example. For example, the imaging device 1 includes a position sensor that detects the position of the first imaging unit 21, and the control unit 7 determines whether the size changing unit 31 is operating based on the detection result (position information) of the position sensor. It may be determined whether or not. This position sensor may be an encoder provided in the size changing unit 31, for example. For example, when the operation of the size changing unit 31 is prohibited or restricted, the control unit 7 may notify the user to that effect by blinking a lamp, sound, or the like. The control unit 7 does not have to adjust the interval between the sample support unit 2 or the calibration reference unit 5 and the first imaging unit 21 when switching between the retracted state and the arrangement state of the calibration reference unit 5.

  Next, the accommodating part 8 is demonstrated. FIG. 5 is a diagram illustrating the accommodating portion 8 of the imaging device 1 according to the present embodiment. The accommodating part 8 accommodates the sample support part 2 and the imaging unit 4 (for example, the first imaging part 21). The accommodating portion 8 has an accommodating space SP for accommodating the sample support portion 2 and the imaging unit 4 therein. The accommodating portion 8 can open the accommodating space SP to the outside. FIG. 5A shows a state where the accommodation space SP is opened (open state), and FIG. 5B shows a state where the accommodation space SP is closed.

  The storage unit 8 is in a state in which the storage space SP is closed (closed state, light shielding state), for example, from the outside of the storage unit 8 (eg, inside the imaging device 1 other than the storage unit 8, outside the imaging device 1, etc.). It functions as a dark box (dark room) that can keep the outside light from being blocked. For example, in the storage unit 8, light (eg, external light, light from an indoor light, natural light) enters (incides) from the outside of the storage unit 8 into an infrared light path in a state where the storage space SP is closed. This is suppressed (reduced). The optical path of the infrared light in the accommodation space SP is, for example, an optical path from the light source unit 11 to the detection range A1 (eg, tissue BT, specimen support unit 2, calibration reference unit 5, illumination region) of the first imaging unit 21; It includes at least part of the optical path from the detection range A1 to the first imaging unit 21. In addition, for example, the accommodating portion 8 suppresses (reduces) external light from entering (entering) the visible light optical path from the outside of the accommodating portion 8 in a state where the accommodating space SP is closed. The optical path of visible light in the accommodation space SP is, for example, the optical path from the visible light source unit 13 to the detection range of the second imaging unit 22 (eg, tissue BT, specimen support unit 2, calibration reference unit 5, illumination region), and this It includes at least part of the optical path from the detection range to the second imaging unit 22. For example, the storage unit 8 has external light from the outside of the storage unit 8 (eg, inside the imaging device 1 other than the storage unit 8, outside the imaging device 1, etc.) to the storage space SP in a state where the storage space SP is closed. (For example, light from room lights, natural light) is prevented (reduced) from entering (incident).

  The accommodating part 8 is provided with the leg part 40, the base part 41, the flame | frame part 42 (it shows with a dashed-two dotted line), the cover part 43, and the door member 44, for example. The leg part 40 contacts the installation surface F (for example, the upper surface of a desk) where the imaging device 1 is installed. The base part 41 is provided on the leg part 40 and supported by the leg part 40. The frame part 42 is provided on the base part 41 and supported by the base part 41. For example, at least a part of the control unit 7, the imaging unit 4, the illumination unit 3, the size changing unit 31, and the door driving unit 45 (described later) is provided in the frame unit 42. The accommodating part 8 includes, for example, a holding part that holds the specimen support part 2. For example, the frame part 42 holds the sample support part 2 rotatably. The accommodating portion 8 includes a holding portion that holds the calibration reference portion 2. For example, the base unit 41 holds the calibration reference unit 2. The calibration reference part 2 is attached to the base part 42 by, for example, screwing. The calibration reference unit 2 may be attached so as to be exchangeable, or may be fixed so as not to be exchangeable. For example, the cover portion 43 is provided on the base portion 41 in a non-contact manner with the frame portion 42 and is supported by the base portion 41. The cover part 43 has an inner surface 43 a facing the inside (accommodating space) of the housing part 8 and an outer surface 43 b facing the outside of the housing part 8. The imaging unit 4 (for example, the first imaging unit 21) is not in contact with the cover unit 43, and is supported so that transmission of force from the cover unit 43 is suppressed (reduced). The cover portion 43 may be in contact with at least a part of the detection portion (eg, the imaging unit 4). For example, an absorbent material (eg, cushion material, sponge, etc.) that absorbs force on the inner surface side of the cover portion 43. ), An elastic body or the like may be provided. For example, when at least one of an absorbent material and an elastic body is provided, transmission of force between the cover unit 43 and the detection unit (for example, the imaging unit 4) is reduced.

  The cover part 43 has an opening 43c that opens the accommodation space SP to the outside. The door member 44 is movable between a position that closes the opening 43c (hereinafter referred to as a closed position) and a position that opens at least a part of the opening 43c to the outside (hereinafter referred to as an open position). The closed position of the door member 44 is, for example, a position where the lower end position of the door member 44 is disposed at or below the lower end position of the opening 15a. The closed position of the door member 44 is a position where the lower end position of the door member 44 is disposed above the lower end position of the opening 15a.

  The imaging device 1 includes a door drive unit 45 that drives a door member, for example. The door drive unit 45 includes an actuator 46 and a transmission unit 47, for example. The actuator 46 includes, for example, an electric motor, and generates a driving force that moves the door member 44. The actuator 46 is controlled by the control unit 7 and generates a driving force specified by a control signal supplied from the control unit 7. The transmission unit 47 transmits the driving force from the actuator 46 to the door member 44. The transmission unit 47 transmits the driving force via a pair of members 48 connected by a magnet or the like, for example. The pair of members 48 are separated from each other when a predetermined force (for example, a force larger than a force attracted by a magnetic force, an impact force) is applied to the housing portion 8. Thereby, transmission of the driving force in the transmission part 47 is cut | disconnected, and the drive of the door member 44 stops.

  The door drive unit 45 includes, for example, a non-contact sensor 49, and the control unit 7 controls the door drive unit 45 according to the detection result of the non-contact sensor 49. The non-contact sensor 49 optically detects a user input (eg, operation), for example. For example, the non-contact sensor 49 emits light to the outside through a window 49 a provided in the cover 43 and detects reflected light of this light. For example, when the user holds his / her hand over the window 49a, the intensity of the reflected light detected by the non-contact sensor 49 changes from less than the threshold value to more than the threshold value. In such a case, the control unit 7 controls the door driving unit 45 to move the door member 44 between the open position and the closed position. For example, the non-contact sensor 49 supplies the detection result to the control unit 7, and the control unit 7 monitors the detection result of the non-contact sensor 49. For example, when the control unit 7 determines that the detection result of the non-contact sensor 49 has changed from less than the threshold value to more than the threshold value in a state where the door member 44 is disposed at the open position, the control unit 7 sends a control signal to the door drive unit 45. . The door drive unit 45 moves, for example, the door member 44 from the open position to the closed position in accordance with this control signal. Similarly, for example, when the control unit 7 determines that the detection result of the non-contact sensor 49 has changed from less than the threshold value to more than the threshold value in a state where the door member 44 is disposed at the closed position, the control unit 7 controls the door drive unit 45. By doing so, the door member 44 is moved from the closed position to the open position.

  For example, the control unit 7 may limit or prohibit the relative movement of the sample support unit 2 and the calibration reference unit 5 by the switching unit 6 while the door driving unit 45 moves the door member 44. For example, the control unit 7 determines whether or not the door driving unit 45 is operating based on a control signal that controls the door driving unit 45. When the control unit 7 determines that the door driving unit 45 is in operation, the control unit 7 may not supply an operation start control signal to the switching unit 6, and may supply the operation start control signal to the door driving unit 45. It may be delayed until the end of the operation. Similarly, for example, the control unit 7 may restrict or prohibit the movement of the door member 44 by the door driving unit 45 while the switching unit 6 relatively moves the sample support unit 2 and the calibration reference unit 5.

  Note that the control unit 7 may determine whether or not the door driving unit 45 is operating based on position information of the door member 44, for example. For example, the imaging device 1 includes a position sensor that detects the position of the door member 44, and the control unit 7 determines whether or not the door driving unit 45 is operating based on the detection result (position information) of the position sensor. May be determined. This position sensor may be an encoder provided in the door drive unit 45, for example. For example, when the control unit 7 prohibits or restricts the operation of the switching unit 6 or restricts or prohibits the movement of the door member 44 by the door driving unit 45, the control unit 7 informs that fact by blinking a lamp or sound. For example, the user may be notified.

  The control unit 7 also, for example, captures the imaging unit 4 (for example, the first imaging unit 21) and the sample support unit 2 (or the calibration reference unit 5) by the size changing unit 31 while the door driving unit 45 moves the door member 44. ) May be restricted or prohibited. For example, when the control unit 7 determines that the door driving unit 45 is in operation, the control unit 7 may not supply an operation start control signal to the size changing unit 31, and may supply the operation start control signal to the door. You may delay until the operation | movement of the drive part 45 is complete | finished. Similarly, for example, while the size changing unit 31 relatively moves the imaging unit 4 (for example, the first imaging unit 21) and the sample support unit 2 (or the calibration reference unit 5), the control unit 7 performs the door driving unit 45. The movement of the door member 44 may be restricted or prohibited. For example, when the operation of the size changing unit 31 is prohibited or restricted, or when the movement of the door member 44 by the door driving unit 45 is restricted or prohibited, the control unit 7 informs that fact by blinking a lamp, The user may be notified by voice or the like.

  Moreover, the imaging device 1 may include an interlock mechanism. For example, the control device 1 may limit or prohibit at least a part of the operation of the imaging device 1 according to the opening / closing state (eg, position, movement) of the door member 44. For example, the control unit 7 may limit or prohibit at least a part of the operation of the imaging device 1 based on the detection result of the non-contact sensor 49. For example, when the non-contact sensor 49 detects an input from the user when switching between the arrangement state and the retracted state of the calibration reference unit 5 (eg, during switching operation), the control unit 7 arranges the calibration reference unit 5. The operation for switching between the state and the evacuation state may be restricted or prohibited. For example, the control unit 7 controls the drive unit (for example, the actuator 36) of the switching unit 6 to electrically limit (in terms of software) the operation of switching between the arrangement state and the retracted state of the calibration reference unit 5, or It may be prohibited. For example, when the non-contact sensor 49 detects an input from the user when switching between the arrangement state and the retracted state of the calibration reference unit 5, the control unit 7 opens the door according to the detection result of the non-contact sensor 49. The movement (opening / closing operation) of the member 21 may be restricted or prohibited. For example, the control unit 7 may limit or prohibit the movement (opening / closing operation) of the door member 21 by controlling the door driving unit 45.

  Similarly to the above switching operation, the imaging device 1 performs at least a part of the detection operation by the detection unit (eg, the imaging unit) and the size operation (movement of the detection unit) by the size changing unit 31 with an interlock mechanism. May be restricted or prohibited. The sensor used in the interlock mechanism may not be the non-contact sensor 49, and may be a sensor that detects position information (eg, position, speed, acceleration) of the door member 44, for example. The sensor used in the interlock mechanism may be, for example, an electronic sensor that electrically detects the position information of the door member 44, or a mechanical sensor that mechanically detects the position information of the door member 44. Alternatively, an electronic sensor and a mechanical sensor may be used in combination.

  The interlock mechanism may be an electrical interlock mechanism that electrically (in terms of software) prohibits or restricts the operation of each unit. The interlock mechanism may be a mechanical interlock mechanism that mechanically (in hardware) prohibits or restricts the operation of each unit. For example, the interlock mechanism may mechanically prohibit or restrict the movement of each part (for example, the imaging unit 4 and the calibration reference part 5) by a member such as a stopper in conjunction with the movement of the door member 44. The interlock mechanism may be a combination of an electrical interlock mechanism and a mechanical interlock mechanism.

  Further, the imaging apparatus 1 may include a switching unit that switches whether to operate the interlock mechanism (on / off of the interlock mechanism). This switching unit may be a mechanical switch, or the control unit 7 may switch the interlock mechanism on and off in response to an operator command. In addition, the electrical interlock mechanism and the mechanical interlock mechanism described above may be switched on / off independently, or may be switched on / off in conjunction with each other.

The inner surface 8 a of the housing portion 8 includes an inner surface 44 a of the door member 44 and an inner surface 43 a of the cover portion 43. For example, the inner surface 8a is in a state of surrounding the accommodation space SP in a state where the door member 44 is disposed at the closed position. The inner surface 8a is suppressed (reduced) in infrared light reflection by a surface treatment such as a coating treatment. The inner surface 8a has, for example, a reflectance of near infrared light of less than 10%, less than 5%, less than 2%, less than 1%, less than 0.5%, or less than 0.1. The reflectance of the inner surface 8a is adjusted in advance by the material of the coating film, for example. The reflectance of the inner surface 8a with respect to infrared light is, for example, the reflectance with respect to infrared light of an object (eg, tissue BT, specimen support unit 2, calibration reference unit 5) disposed in the detection range A1 of the first imaging unit 21. Lower than. For example, the reflectance R is expressed by the following formula (1) using the refractive index N1 of one material and the other refractive index N0 with respect to the interface.
R = (N0−N1) ^ 2 / (N0 + N1) (1)

  FIG. 6 is a diagram illustrating the exhaust port 50 of the imaging device 1 according to the embodiment. In the present embodiment, the accommodating portion 8 includes an exhaust port 50 that exhausts gas in a space (accommodating space SP in FIG. 5) in which the specimen support portion 2 is accommodated. The exhaust port 50 is provided, for example, on the back side (the side opposite to the opening 43c) of the housing portion 8. The exhaust port 50 is provided on a predetermined surface (eg, inner surface) of the housing portion 8 such as a side surface or a bottom surface of the housing portion 8 and communicates with the housing space SP of FIG. For example, when the tissue BT is fixed with a tissue fixing solution such as a formalin solution, the imaging apparatus 1 can exhaust gas (eg, formaldehyde) volatilized from the tissue fixing solution from the exhaust port 50 to the outside. Moreover, the imaging device 1 can release the heat of the accommodation space SP to the outside by exhausting the gas in the accommodation space SP. For example, the exhaust port 50 may be provided with an air supply unit such as a fan or a suction device that sends the gas in the accommodation space SP to the outside. Moreover, the exhaust port 50 may be connected to said air supply part via flow paths, such as a hose and piping, for example. Note that the imaging device 1 may be capable of processing the gas in the accommodation space SP (eg, filtering processing) and exhausting the processed gas to the outside.

  In addition, the structure of the above-mentioned accommodating part 8 is an example, and can be changed suitably. For example, a part of the imaging device 1 may not be accommodated in the accommodation unit 8. For example, at least a part of the control unit 7, the door driving unit 45, and the size changing unit 31 may be provided outside the housing unit 8 or may be externally attached to the housing unit 8. Further, the imaging device 1 may not include at least a part of the accommodating portion 8. For example, when the imaging device 1 is used in a dark room or the like, the storage unit 8 does not have to block outside light. For example, when the storage unit 8 does not block external light, the storage unit 8 may not include the door member 44 and the door drive unit 45. Further, for example, when the imaging device 1 is used in a draft chamber or the like, the accommodating portion 8 may not include the exhaust port 50.

  Next, an example of an imaging method will be described based on the configuration of the imaging device 1 described above. The imaging method according to the present embodiment includes, for example, supporting a biological tissue BT on a specimen support unit, imaging a tissue with an imaging unit by irradiation with infrared light, and a calibration standard used for calibration of the imaging unit. An arrangement state in which at least one of the unit 5 and the sample support unit 2 is moved and the calibration reference unit is disposed within the detection range (eg, in the field of view) of the imaging unit, and the calibration reference unit is the detection range of the imaging unit And switching to a retracted state arranged outside (eg, out of the field of view).

  FIG. 7 is a flowchart illustrating an example of an imaging method according to the embodiment. In the present embodiment, the imaging device 1 calibrates the first imaging unit 21 prior to imaging the tissue BT, for example. In step S <b> 1, the control unit 7 controls the switching unit 6 to move the sample support unit 2 to place the calibration reference unit 5 in the detection range A <b> 1 of the first imaging unit 21. In step S <b> 2, the control unit 7 irradiates the calibration reference unit 5 by irradiating infrared light from the light source unit 11 in a state where the door member 44 of the housing unit 8 is disposed at the closed position, and the first imaging unit 21. Then, the calibration reference unit 5 is imaged. When the captured image obtained in step S2 is used, an output of each pixel of the first imaging unit 21 with respect to a predetermined light amount is obtained. For example, the calibration reference unit 5 is formed so that the variation (dispersion) of the spatial distribution (in-plane distribution) of the reflectance is not more than a predetermined value. For example, in the detection result (captured image) of the detection unit (imaging unit 4), the distribution of detection values (eg, pixel values) is the characteristics (eg, sensitivity, S / N ratio) of the light receiving element (pixels of the image sensor 16). , The relationship of the output to the light quantity). For example, the pixel of the image sensor 16 corresponding to a relatively dark portion in the captured image may have a lower sensitivity than other pixels. For such a pixel, for example, by increasing the gain or adding a positive offset, the output for a predetermined amount of light can be aligned with other pixels. Further, for example, the calibration reference unit 5 is formed so that the reflectance distribution in the predetermined wavelength band (the reflectance distribution with respect to the wavelength) becomes a predetermined distribution. For example, in the detection result (captured image) of the detection unit (imaging unit 4), the distribution of the detection value (eg, pixel value) and the distribution of the reflectance with respect to the wavelength of the calibration reference unit 5 are compared. The characteristics (eg, sensitivity) of the light receiving element (pixel of the image sensor 16) can be obtained. For example, the characteristics of the light receiving element with respect to a predetermined wavelength can be corrected (calibrated) based on the wavelength dependence of the characteristics of the light receiving element obtained based on the captured image. In step S <b> 3, the control unit 7 places the calibration reference unit 5 in the first imaging unit 21 in a state in which the door member 44 of the housing unit 8 is disposed at the closed position and irradiation of infrared light from the light source unit 11 is stopped. To image. When the captured image (dark image) obtained in step S3 is used, the output of each pixel of the first imaging unit 21 when the light amount is almost zero is obtained. For example, the pixel of the image sensor 16 corresponding to a relatively bright part in the captured image may have higher sensitivity or more noise than other pixels. For such a pixel, for example, by reducing the gain or adding a negative offset, the output for a predetermined light amount (for example, the light amount is almost 0) can be aligned with other pixels. In step S4, the control unit 7 calibrates the first imaging unit 21 using the captured image in step S2 and the captured image in step S3. In addition, the imaging device 1 uses the visible light source unit 13 instead of the light source unit 11 and uses the second imaging unit 22 instead of the first imaging unit 21 to perform the above-described steps S1 to S4. The second imaging unit 22 can also be calibrated.

  Next, the imaging device 1 uses the calibrated first imaging unit 21 to image the tissue BT by the processing from step S5. In step S <b> 5, the control unit 7 controls the switching unit 6 to place the sample support unit 2 in the detection range A <b> 1 of the first imaging unit 21. In step S <b> 6, the biological tissue BT is placed on the specimen support unit 2. In step S7, the control unit 7 causes the light source unit 11 to irradiate the tissue BT on the specimen support unit 2 with infrared light in a state where the door member 44 of the storage unit 8 is disposed at the closed position, and the first imaging unit. 21 causes the tissue BT to be imaged. In step S8, the control unit 7 outputs the captured image data obtained in step S7 to the outside.

  Note that the control unit 7 may execute the calibration process of Steps S1 to S4 every time one image is taken, or every time a predetermined number of times is taken. In addition, the above calibration processing is performed when at least a part of imaging conditions (eg, shutter speed of the image sensor 24, size of the detection range A1, wavelength of infrared light, intensity of infrared light) is changed. It may be done. The calibration process may be performed every time the imaging apparatus 1 is turned on (every time it is activated), or may be performed every time the imaging apparatus 1 is activated a predetermined number of times. Further, the calibration process may be executed every time a predetermined period elapses. Moreover, the control part 7 may perform said calibration process according to a user's instruction | command. In addition, the control unit 7 may notify the user of the recommended timing for executing the calibration process based on, for example, the number of times of imaging from the previous calibration process, the elapsed time, and the imaging conditions.

  Next, another form of the switching unit 6 will be described. FIG. 8A is a diagram illustrating the imaging apparatus 1 according to the first modification. 8A is arranged in the optical path between the first imaging unit 21 and the specimen support unit 2 in the arrangement state. In the arrangement state of the calibration reference unit 5, the sample support unit 2 is arranged outside the detection range A <b> 1 of the first imaging unit 21 by the optical path between the first imaging unit 21 being blocked by the calibration reference unit 5. . The switching unit 6 can rotate the calibration reference unit 5 around a rotation axis 51 that is non-parallel (eg, vertical) to the optical axis 21 a of the first imaging unit 21. When the control unit 7 switches from the arrangement state of the calibration reference unit 5 to the retracted state, the control unit 7 controls the switching unit 6 to rotate the calibration reference unit 5 around the rotation axis 51, thereby causing the calibration reference unit 5 to move to the first imaging unit. Retract from the optical path between 21 and the specimen support 2. In the retracted state of the calibration reference unit 5, the sample support unit 2 is disposed within the detection range A <b> 1 of the first imaging unit 21 because the optical path to the first imaging unit 21 is not blocked by the calibration reference unit 5. The For example, when it is assumed that the time for the calibration reference unit 5 in the retracted state is longer than the time in the arrangement state, the switching unit 6 moves the calibration reference unit 5 to the back side of the imaging apparatus 1 (see FIG. 1 may be rotated to the + Y side). When rotating at least one of the calibration reference unit 5 and the sample support unit 2, the rotation axis is set in an arbitrary direction that is not parallel to the optical axis 21 a of the first imaging unit 21.

  FIG. 8B is a diagram illustrating the imaging apparatus 1 according to the second modification. 8B is disposed in the optical path between the calibration reference unit 5 and the first imaging unit 21 when the calibration reference unit 5 is in the retracted state. In the retracted state of the calibration reference unit 5, the calibration reference unit 5 is disposed outside the detection range A <b> 1 of the first imaging unit 21 by blocking the optical path between the first imaging unit 21 and the sample support unit 2. . The specimen support part 2 can be divided into a plurality of parts (first part, second part) 2a, 2b. The switching unit 6 can rotate the component 2 a around the rotation shaft 52 and can rotate the component 2 b around the rotation shaft 53. For example, the rotation shaft 52 is provided at one end portion of the sample support portion 2, and the rotation shaft 53 is provided at the other end portion of the sample support portion 2. The control unit 7 controls the switching unit 6 to rotate the component 2a away from the calibration reference unit 5 (counterclockwise in the drawing) when switching from the arrangement state of the calibration reference unit 5 to the retracted state. Retreat from the optical path between the reference unit 5 and the first imaging unit 21. In addition, the control unit 7 rotates the component 2 b away from the calibration reference unit 5 (clockwise in the figure) and retracts it from the optical path between the calibration reference unit 5 and the first imaging unit 21. Thereby, the sample support part 2 is divided into a plurality of parts 2 a and 2 b and retracts from the optical path between the calibration reference part 5 and the first imaging part 21. The calibration reference unit 5 is arranged in the detection range A <b> 1 of the first imaging unit 21 because the optical path between the calibration imaging unit 5 and the first imaging unit 21 is not blocked by the sample support unit 2. In the second modification, the calibration reference unit 5 may be disposed in the optical path between the sample support unit 2 and the first image pickup unit 21. In this case, the control unit 7 may replace the sample support unit 2. Alternatively, the calibration reference unit 5 may be divided into a plurality of parts and retracted from the optical path between the sample support unit 2 and the first imaging unit 21.

  FIG. 8C is a diagram illustrating the imaging apparatus 1 according to the third modification. The specimen support unit 2 in FIG. 8C is disposed in the optical path between the calibration reference unit 5 and the first imaging unit 21 in the retracted state of the calibration reference unit 5. In the retracted state of the calibration reference unit 5, the calibration reference unit 5 is disposed outside the detection range A <b> 1 of the first imaging unit 21 by blocking the optical path between the first imaging unit 21 and the sample support unit 2. . The switching unit 6 translates the sample support unit 2, and can retract the sample support unit 2 from the optical path between the first imaging unit 21 and the calibration reference unit 5. The control unit 7 controls the switching unit 6 to switch the specimen support unit 2 from the optical path between the calibration reference unit 5 and the first imaging unit 21 when switching the calibration reference unit 5 from the retracted state to the arrangement state. Evacuate. The calibration reference unit 5 is arranged in the detection range A <b> 1 of the first imaging unit 21 because the optical path between the calibration imaging unit 5 and the first imaging unit 21 is not blocked by the sample support unit 2. In the third modification, the calibration reference unit 5 may be disposed in the optical path between the sample support unit 2 and the first image pickup unit 21. In this case, the control unit 7 may replace the sample support unit 2. Alternatively, the calibration reference unit 5 may be moved in parallel and retracted from the optical path between the sample support unit 2 and the first imaging unit 21.

  FIG. 9A is a diagram illustrating an imaging apparatus 1 according to a fourth modification. The specimen support unit 2 in FIG. 9A is disposed in the optical path between the calibration reference unit 5 and the first imaging unit 21 in the retracted state of the calibration reference unit 5. In the retracted state of the calibration reference unit 5, the calibration reference unit 5 is disposed outside the detection range A <b> 1 of the first imaging unit 21 by blocking the optical path between the first imaging unit 21 and the sample support unit 2. . The specimen support part 2 can be divided into a plurality of parts 2a and 2b. The switching unit 6 translates the components 2a and 2b, respectively, and can retract the components 2a and 2b from the optical path between the first imaging unit 21 and the calibration reference unit 5, respectively. When the control unit 7 switches the calibration reference unit 5 from the retracted state to the arrangement state, the control unit 7 controls the switching unit 6 to divide the sample support unit 2 into components 2a and 2b, and each of the components 2a and 2b is imaged first. The optical path between the unit 21 and the calibration reference unit 5 is retracted. The calibration reference unit 5 is arranged in the detection range A <b> 1 of the first imaging unit 21 because the optical path between the calibration imaging unit 5 and the first imaging unit 21 is not blocked by the sample support unit 2. In the fourth modification, the calibration reference unit 5 may be disposed in the optical path between the sample support unit 2 and the first imaging unit 21, and in this case, the control unit 7 replaces the sample support unit 2. Alternatively, the calibration reference unit 5 may be divided into a plurality of parts and retracted from the optical path between the sample support unit 2 and the first imaging unit 21.

  FIG. 9B is a diagram illustrating the imaging apparatus 1 according to the fifth modification. The calibration reference unit 5 in FIG. 9B has a smaller area than the detection range A1 of the first imaging unit 21. The calibration reference unit 5 is disposed on the same side as the first imaging unit 21 with respect to the sample support unit 2. The switching unit 6 can move the calibration reference unit 5 across the optical path between the sample support unit 2 and the first imaging unit 21. The control unit 7 controls the switching unit 6 to switch the calibration reference unit 5 between the first imaging unit 21 and the sample support unit 2 when switching the calibration reference unit 5 from the retracted state to the arrangement state. Place it in a part. In the arrangement state of the calibration reference unit 5, the control unit 7 controls the switching unit 6 to switch the position of the calibration reference unit 5 in the detection range A <b> 1 of the first imaging unit 21, while the calibration reference unit 5 is connected to the first imaging unit 21. 5 is imaged. For example, the control unit 7 performs calibration for each region where the calibration reference unit 5 is captured in the captured image of the first imaging unit 21. In addition, the sample support part 2 may move during the movement of the calibration reference part 5, or may not move. For example, the sample support unit 2 may be provided on the same moving body as the calibration reference unit 5 and may move together with the calibration reference unit 2. The standard support unit 2 may move in a different direction from the calibration reference unit 5 or may move at a speed different from that of the calibration reference unit 5.

  FIG. 9C is a diagram illustrating an imaging apparatus 1 according to a sixth modification. The calibration reference portion 5 in FIG. 9C is provided in a part of the sheet-like optical member 54, for example. The optical member 54 is stretched over rollers 55 and 56 provided at both ends of the specimen support 2 and is provided so as to cover both the front and back surfaces of the specimen support 2. The switching unit 6 rotates or circulates the optical member 54 around the sample support unit 2 by rotating at least one of the rollers 55 and 56. For example, the calibration reference unit 5 is provided in a part of the optical member 54 in the circumferential direction. FIG. 9C corresponds to the retracted state of the calibration reference unit 5, and the calibration reference unit 5 is disposed on the opposite side of the first imaging unit 21 with respect to the specimen support unit 2. When the control unit 7 switches the calibration reference unit 5 from the retracted state to the arrangement state, the control unit 7 controls the switching unit 6 to rotate the optical member 54, thereby causing the calibration reference unit 5 to detect the detection range A <b> 1 of the first imaging unit 21. To be placed. In addition, when the control unit 7 switches the calibration reference unit 5 from the retracted state to the arrangement state, the control unit 7 controls the switching unit 6 to further circulate the optical member 54, thereby causing the calibration reference unit 5 to move to the first imaging unit 21. Retreat from the detection range A1.

  The imaging device 1 as described above is used in an imaging system including a display device and an image processing device, for example. For example, the image processing apparatus performs image processing on a captured image of the tissue BT captured by the imaging apparatus 1 to generate an image. In addition, the imaging system causes the display device to display at least one of a captured image of the tissue BT captured by the imaging device 1 and an image generated by the image processing device. In addition, for example, the imaging device 1 according to the present embodiment can be used for a medical device for supporting pathological diagnosis using infrared light (eg, a pathological imaging device using infrared light).

  The technical scope of the present invention is not limited to the aspects described in the above-described embodiments. One or more of the requirements described in the above embodiments and the like may be omitted. In addition, the requirements described in the above-described embodiments and the like can be combined as appropriate. In addition, as long as it is permitted by law, the disclosure of all documents cited in the above-described embodiments and the like is incorporated as a part of the description of the text.

DESCRIPTION OF SYMBOLS 1 ... Imaging device, 2 ... Sample support part, 5 ... Calibration reference | standard part, 6 ... Switching part,
7 ... control unit, 8 ... housing unit, 8a ... inner surface, 11 ... light source unit,
12 ... Holding member, 13 ... Visible light source unit, 14 ... Light source moving unit,
15 ... Diffusing member, 16 ... Multiple light sources, 21 ... First imaging unit,
21a ... Optical axis, 31 ... Size changing part, 44 ... Door member, 45 ... Door drive part,
49 ... Non-contact sensor, 50 ... Exhaust port, A1 ... Detection range, BT ... Tissue

Claims (21)

A specimen support that supports biological tissue;
A detection unit for detecting light emitted from the tissue by irradiation with infrared light;
A calibration reference part used for calibration of the detection part;
An arrangement state in which at least one of the calibration reference part and the sample support part is moved, the calibration reference part is arranged within the detection range of the detection part, and the calibration reference part is outside the detection range of the detection part An imaging apparatus comprising: a controller that switches between a retracted state and a disposition state.   The imaging apparatus according to claim 1, further comprising a switching unit that is controlled by the control unit and relatively moves the calibration reference unit and the sample support unit.   The sample support unit is disposed outside the detection range of the detection unit when switching from the arrangement state to the retracted state, and is disposed within the detection range of the detection unit when switching from the retraction state to the arrangement state. The imaging device according to claim 1 or 2.   The said calibration reference | standard part is located under the said sample support part in the said retracted state, and is arrange | positioned so that one surface may be covered with the said sample support part. Imaging device.   The imaging device according to any one of claims 1 to 4, wherein the specimen support part and the calibration reference part are arranged along an optical axis direction of the detection part.   6. The control unit according to claim 1, wherein the control unit rotates at least one of the sample support unit and the calibration reference unit around an axis that is not parallel to the optical axis direction of the detection unit. Imaging device. In the retracted state, the calibration reference part is held on the side opposite to the detection part with respect to the specimen support part,
The imaging device according to any one of claims 1 to 6, wherein the control unit rotates the sample support unit when switching to the arrangement state.   8. The apparatus according to claim 1, further comprising a size changing unit that relatively moves the detection unit and the sample support unit in an optical axis direction of the detection unit and changes a size of the detection range. Imaging device. A light source unit for emitting the infrared light;
The imaging device according to claim 1, further comprising: a diffusion member that diffuses the infrared light from the light source unit.   The imaging device according to claim 9, wherein the light source unit includes a plurality of light sources that emit the infrared light having different wavelengths.   The imaging device according to claim 9, further comprising a light source moving unit that changes an irradiation angle of the infrared light with respect to the tissue.   The imaging device according to claim 9, further comprising a visible light source unit that irradiates the tissue with visible light.   The imaging device according to claim 12, wherein the light source unit and the visible light source unit are held by the same member.   The diffusing member is configured integrally with the light source unit and the visible light source unit, and is disposed between the sample support unit, the light source unit, and the visible light source unit. The imaging device according to item.   The imaging device according to claim 1, further comprising a housing unit that houses the sample support unit and the detection unit and blocks external light incident on an optical path of the infrared light. The accommodating portion has an inner surface surrounding an optical path between the specimen support portion and the detection portion,
The imaging device according to claim 15, wherein the inner surface is configured to reduce reflection of light emitted from the tissue by irradiation with the infrared light.   The imaging device according to claim 15 or 16, wherein the storage unit includes a door member capable of opening a space for storing the specimen support unit to the outside, and a door drive unit that drives the door member.   The imaging device according to claim 17, wherein the door driving unit includes a non-contact sensor and drives the door member according to a detection result of the non-contact sensor.   The imaging device according to claim 17 or 18, wherein the door driving unit stops driving the door member when a predetermined force is applied to the housing unit. The accommodating portion includes a cover portion including an outer surface facing the outside,
The imaging device according to any one of claims 13 to 17, wherein the detection unit is supported so as to reduce transmission of force from the cover unit.   The imaging device according to any one of claims 15 to 20, wherein the storage unit includes an exhaust port that exhausts gas in a space in which the sample support unit is stored to the outside.

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