JP2017000088A - 植物保護能力を有する微生物 - Google Patents
植物保護能力を有する微生物 Download PDFInfo
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- JP2017000088A JP2017000088A JP2015118395A JP2015118395A JP2017000088A JP 2017000088 A JP2017000088 A JP 2017000088A JP 2015118395 A JP2015118395 A JP 2015118395A JP 2015118395 A JP2015118395 A JP 2015118395A JP 2017000088 A JP2017000088 A JP 2017000088A
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Abstract
Description
[態様1]配列番号1又は2で表される塩基配列と95%以上の同一性を有する塩基配列からなる核酸を含む、微生物。
[態様2]シュードモナス(Pseudomonas)属細菌である、態様1の微生物。
[態様3]phl遺伝子クラスター及びhcn遺伝子クラスターを有する、態様1又は2の微生物。
[態様4]plt遺伝子クラスター及びprn遺伝子クラスターを有さない、態様1〜3のいずれか1の微生物。
[態様5]rzx類縁体遺伝子クラスターをさらに有する、態様1〜4のいずれか1の微生物。
[態様6]シュードモナス(Pseudomonas)sp. Os17株(受領番号NITE AP−02053)又はシュードモナス(Pseudomonas)sp. St29株(受領番号NITE AP−02054)である、態様1の微生物。
[態様7]態様1〜6のいずれか1の微生物を含む、生物農薬。
[態様8]植物保護剤である、態様7の生物農薬。
[態様9]ピシウム(Pythium)属菌又はフザリウム(Fusarium)属菌に関連する植物病に対する植物保護剤である、態様8の生物農薬。
[態様10]態様1〜6のいずれか1の微生物を植物に接触させる工程を含む、植物の保護方法。
本発明は、新規な微生物を提供するものであり、本発明の微生物は、配列番号1又は2で表される塩基配列と95%以上の同一性を有する塩基配列からなる核酸を含むことを特徴とする。
本発明はまた、上記(1)で説明した本発明の微生物を含む生物農薬を提供することができる。本明細書において生物農薬とは、生物を利用することにより得られる農薬を意味する。本発明の生物農薬は、本発明の微生物を利用することから微生物農薬と称することもできる。
本発明はまた、上記(1)で説明した本発明の微生物を利用した植物の保護方法を提供することができる。本発明の植物の保護方法は、本発明の微生物又は生物農薬を植物に接触させる工程を含むことを特徴とする。
P. protegensは普遍的に生息すると言われながら、アジアにおける単離報告例がこれまでになかった。そこで本研究では、P. protegens近縁種の日本国内分離株からの探索を試みた。
(2)−1.抗菌性試験
新たに単離されたOs17株及びSt29株の抗菌作用を調べるため、Bacillus subtilis及び植物病原菌であるFusarium oxysporumに対する抗菌性試験を行った。
オートクレーブして滅菌水を吸水させたアワを、滅菌シャーレ1枚につき約14gずつ分けて入れ、ピシウム菌(Pythium ultimum、農業生物資源ジーンバンク(http://www.gene.affrc.go.jp/index_j.php)のMAFF番号、MAFF425494)を植菌して、27℃、暗黒下で1週間培養した。滅菌水をしみ込ませた滅菌濾紙の上に、表面滅菌したキュウリの種子(品種:新ときわ地這)を並べ、26℃、24時間、暗黒下に置いて発芽、発根させた。検定に供するPseudomonas属細菌のシングルコロニーをNYB培地(25g/L Nutrient broth (Oxoid)、5g/L Yeast extract (Oxoid))に植菌し、振とう培養した(30℃、180 rpm、24時間)。Pseudomonas属細菌の培養液を遠心にかけて集菌し、滅菌水に懸濁した。OD600=0.1になるよう、50mL容のファルコンチューブにて滅菌水で調整した。バーミキュライト((株)白元)に水を加えてなじませた。無処理用のバーミキュライトを取り分けておき、残りのバーミキュライトについて、1Lにつきピシウム菌感染アワを7gの割合で加え、よく撹拌した。バーミキュライトを、100mL容のフラスコに30mLずつ入れた。キュウリの種を3粒ずつ、発根したもののみバーミキュライトの上にまき、その種子の上にPseudomonas属細菌の調整済み水溶液を3mLずつ滴下した。上から無処理のバーミキュライトを5mLずつかぶせ、シリコン栓をして、明期16時間、暗期8時間、25℃にて1週間栽培した。栽培後、キュウリの生残数を数え、地上部と根に分けて重さを測定した。
新たに単離されたOs17株及びSt29株の機能性及び特性を調べるため、各菌株について全ゲノムの塩基配列決定を行った。配列決定に供するため、Os17株及びSt29株からのゲノムDNAの抽出を、キアゲンGenomic DNA buffer set and genomic-tipを用いて行った。これを用い、解析用の8 kb paired end ライブラリを作製し、Roche GS FLX Titanium による次世代シークエンシング解析を行った。シークエンシング解析後、アセンブル解析を実施し、アセンブル結果の最適化を行うことにより、ギャップ無しの単一コンティグ化、および環状化に成功した。
Os17株及びSt29株のゲノムデータは下記の通りであり、これまでに入手していたCab57株と合わせて下表に示す。
本研究では、Os17株、St29株及びCab57株の3菌株に関して、BLASTpを利用して(カットオフ値:60%)タンパク質コード配列(CDS)の比較解析を行った(図4)。Os17株に特異的な256個のCDS、及びSt29株に特異的な347個のCDSの中に植物保護能力の差の原因となる遺伝子が含まれるものと考えられる。また、Os17株及びSt29株に特異的な519個のCDSの中に、これらの種特有の植物保護能力の高い遺伝子が含まれると考えられる。
上記の結果からOs17株におけるrzx類縁体遺伝子クラスターの重要性を調べるために、Os17株のrzxB欠損変異株を作製し、野生株との抗菌作用の比較を行った。rzxB欠損変異株は次の通り作製した。下記のプライマーRzxBUF/RzxBUR及びRzxBDF/RzxBDRを用いて、rzxB遺伝子の6790位〜7580位の790bpの領域及び9400位〜10180位の780bpの領域をそれぞれPCRにより増幅した。なおPCRにおいては、高正確性DNAポリメラーゼKOD Plus(東洋紡)を用い、テンプレートとしてOs17株のゲノムDNAを使用した。
Claims (10)
- 配列番号1又は2で表される塩基配列と95%以上の同一性を有する塩基配列からなる核酸を含む、微生物。
- シュードモナス(Pseudomonas)属細菌である、請求項1に記載の微生物。
- phl遺伝子クラスター及びhcn遺伝子クラスターを有する、請求項1又は2に記載の微生物。
- plt遺伝子クラスター及びprn遺伝子クラスターを有さない、請求項1〜3のいずれか1項に記載の微生物。
- rzx類縁体遺伝子クラスターをさらに有する、請求項1〜4のいずれか1項に記載の微生物。
- シュードモナス(Pseudomonas)sp. Os17株(受領番号NITE AP−02053)又はシュードモナス(Pseudomonas)sp. St29株(受領番号NITE AP−02054)である、請求項1に記載の微生物。
- 請求項1〜6のいずれか1項に記載の微生物を含む、生物農薬。
- 植物保護剤である、請求項7に記載の生物農薬。
- ピシウム(Pythium)属菌又はフザリウム(Fusarium)属菌に関連する植物病に対する植物保護剤である、請求項8に記載の生物農薬。
- 請求項1〜6のいずれか1項に記載の微生物を植物に接触させる工程を含む、植物の保護方法。
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101848579B1 (ko) * | 2017-01-10 | 2018-04-12 | 강원대학교산학협력단 | 항균 활성을 가지는 슈도모나스 프로테겐스 jr52 균주 및 이의 용도 |
CN112111426A (zh) * | 2020-09-22 | 2020-12-22 | 河南科技学院 | 一种亚麻假单胞菌及其在大葱紫斑病生防中的应用 |
WO2021028911A1 (en) * | 2019-08-14 | 2021-02-18 | Lavie Bio Ltd. | Bacterial strains having fungicidal activity, compositions comprising same and use thereof |
JP7508106B2 (ja) | 2020-12-14 | 2024-07-01 | 国立研究開発法人農業・食品産業技術総合研究機構 | 植物成長促進又は植物保護の能力を有する微生物 |
-
2015
- 2015-06-11 JP JP2015118395A patent/JP6653097B2/ja active Active
Non-Patent Citations (2)
Title |
---|
TAKEUCHI K. ET AL.: "Complete genome sequence of the biocontrol strain Pseudomonas protegens Cab57 discovered in Japan re", PLOS ONE, vol. 9(4), JPN6018045222, 2014, pages e93683 * |
竹内香純ら: "Pseudomonas sp.Os17の植物保護能力にはリゾキシン類縁体が寄与する", 日本植物病理学会大会プログラム・講演要旨予稿集, vol. 15, JPN6018045221, 10 March 2015 (2015-03-10), pages 106 * |
Cited By (5)
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---|---|---|---|---|
KR101848579B1 (ko) * | 2017-01-10 | 2018-04-12 | 강원대학교산학협력단 | 항균 활성을 가지는 슈도모나스 프로테겐스 jr52 균주 및 이의 용도 |
WO2021028911A1 (en) * | 2019-08-14 | 2021-02-18 | Lavie Bio Ltd. | Bacterial strains having fungicidal activity, compositions comprising same and use thereof |
CN112111426A (zh) * | 2020-09-22 | 2020-12-22 | 河南科技学院 | 一种亚麻假单胞菌及其在大葱紫斑病生防中的应用 |
CN112111426B (zh) * | 2020-09-22 | 2022-10-21 | 河南科技学院 | 一种亚麻假单胞菌及其在大葱紫斑病生防中的应用 |
JP7508106B2 (ja) | 2020-12-14 | 2024-07-01 | 国立研究開発法人農業・食品産業技術総合研究機構 | 植物成長促進又は植物保護の能力を有する微生物 |
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