JP2016535601A5 - - Google Patents
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- JP2016535601A5 JP2016535601A5 JP2016552246A JP2016552246A JP2016535601A5 JP 2016535601 A5 JP2016535601 A5 JP 2016535601A5 JP 2016552246 A JP2016552246 A JP 2016552246A JP 2016552246 A JP2016552246 A JP 2016552246A JP 2016535601 A5 JP2016535601 A5 JP 2016535601A5
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- 239000003153 chemical reaction reagent Substances 0.000 claims 8
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- 235000013622 meat product Nutrition 0.000 claims 6
- -1 BIO Chemical compound 0.000 claims 5
- 230000001973 epigenetic Effects 0.000 claims 5
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- 239000003112 inhibitor Substances 0.000 claims 4
- 244000144972 livestock Species 0.000 claims 4
- 230000001114 myogenic Effects 0.000 claims 4
- AQGNHMOJWBZFQQ-UHFFFAOYSA-N CT 99021 Chemical compound CC1=CNC(C=2C(=NC(NCCNC=3N=CC(=CC=3)C#N)=NC=2)C=2C(=CC(Cl)=CC=2)Cl)=N1 AQGNHMOJWBZFQQ-UHFFFAOYSA-N 0.000 claims 3
- 102000019058 Glycogen Synthase Kinase 3 beta Human genes 0.000 claims 3
- 108010051975 Glycogen Synthase Kinase 3 beta Proteins 0.000 claims 3
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- 102000003964 Histone deacetylases Human genes 0.000 claims 2
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- KWGKDLIKAYFUFQ-UHFFFAOYSA-M Lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 claims 2
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- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 claims 1
- VPVLEBIVXZSOMQ-UHFFFAOYSA-N 3-[[6-(3-aminophenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy]phenol Chemical compound NC1=CC=CC(C=2NC3=NC=NC(OC=4C=C(O)C=CC=4)=C3C=2)=C1 VPVLEBIVXZSOMQ-UHFFFAOYSA-N 0.000 claims 1
- JHSXDAWGLCZYSM-UHFFFAOYSA-N 4-(4-chloro-2-methylphenoxy)-N-hydroxybutanamide Chemical compound CC1=CC(Cl)=CC=C1OCCCC(=O)NO JHSXDAWGLCZYSM-UHFFFAOYSA-N 0.000 claims 1
- JTDYUFSDZATMKU-UHFFFAOYSA-N 6-(1,3-dioxobenzo[de]isoquinolin-2-yl)-N-hydroxyhexanamide Chemical compound C1=CC(C(N(CCCCCC(=O)NO)C2=O)=O)=C3C2=CC=CC3=C1 JTDYUFSDZATMKU-UHFFFAOYSA-N 0.000 claims 1
- MDZCSIDIPDZWKL-UHFFFAOYSA-N 6-N-[2-[[4-(2,4-dichlorophenyl)-5-imidazol-1-ylpyrimidin-2-yl]amino]ethyl]-3-nitropyridine-2,6-diamine Chemical compound C1=C([N+]([O-])=O)C(N)=NC(NCCNC=2N=C(C(=CN=2)N2C=NC=C2)C=2C(=CC(Cl)=CC=2)Cl)=C1 MDZCSIDIPDZWKL-UHFFFAOYSA-N 0.000 claims 1
- VUWMSJQZENJPHO-UHFFFAOYSA-N 7-oxo-7-(N-phenylanilino)heptanoic acid Chemical compound C=1C=CC=CC=1N(C(=O)CCCCCC(=O)O)C1=CC=CC=C1 VUWMSJQZENJPHO-UHFFFAOYSA-N 0.000 claims 1
- MAUCONCHVWBMHK-UHFFFAOYSA-N Abexinostat Chemical compound O1C2=CC=CC=C2C(CN(C)C)=C1C(=O)NCCOC1=CC=C(C(=O)NO)C=C1 MAUCONCHVWBMHK-UHFFFAOYSA-N 0.000 claims 1
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- RFLHBLWLFUFFDZ-UHFFFAOYSA-N BML-210 Chemical compound NC1=CC=CC=C1NC(=O)CCCCCCC(=O)NC1=CC=CC=C1 RFLHBLWLFUFFDZ-UHFFFAOYSA-N 0.000 claims 1
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- XQTWDDCIUJNLTR-CVHRZJFOSA-N Doxycycline Chemical compound O.O=C1C2=C(O)C=CC=C2[C@H](C)[C@@H]2C1=C(O)[C@]1(O)C(=O)C(C(N)=O)=C(O)[C@@H](N(C)C)[C@@H]1[C@H]2O XQTWDDCIUJNLTR-CVHRZJFOSA-N 0.000 claims 1
- 229960003722 Doxycycline Drugs 0.000 claims 1
- 210000001671 Embryonic Stem Cells Anatomy 0.000 claims 1
- 102000001267 GSK3 Human genes 0.000 claims 1
- 108060006662 GSK3 Proteins 0.000 claims 1
- YALNUENQHAQXEA-UHFFFAOYSA-N Givinostat Chemical compound C1=CC2=CC(CN(CC)CC)=CC=C2C=C1COC(=O)NC1=CC=C(C(=O)NO)C=C1 YALNUENQHAQXEA-UHFFFAOYSA-N 0.000 claims 1
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- 101700014819 MYOG Proteins 0.000 claims 1
- 241001465754 Metazoa Species 0.000 claims 1
- 210000003205 Muscles Anatomy 0.000 claims 1
- HRNLUBSXIHFDHP-UHFFFAOYSA-N N-(2-aminophenyl)-4-[[(4-pyridin-3-ylpyrimidin-2-yl)amino]methyl]benzamide Chemical compound NC1=CC=CC=C1NC(=O)C(C=C1)=CC=C1CNC1=NC=CC(C=2C=NC=CC=2)=N1 HRNLUBSXIHFDHP-UHFFFAOYSA-N 0.000 claims 1
- OYKBQNOPCSXWBL-SNAWJCMRSA-N N-hydroxy-3-[(E)-3-(hydroxyamino)-3-oxoprop-1-enyl]benzamide Chemical compound ONC(=O)\C=C\C1=CC=CC(C(=O)NO)=C1 OYKBQNOPCSXWBL-SNAWJCMRSA-N 0.000 claims 1
- GOVYBPLHWIEHEJ-UHFFFAOYSA-N N-hydroxy-4-[(2-methyl-3,4-dihydro-1H-pyrido[4,3-b]indol-5-yl)methyl]benzamide Chemical compound C1N(C)CCC2=C1C1=CC=CC=C1N2CC1=CC=C(C(=O)NO)C=C1 GOVYBPLHWIEHEJ-UHFFFAOYSA-N 0.000 claims 1
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- FWZRWHZDXBDTFK-ZHACJKMWSA-N Panobinostat Chemical compound CC1=NC2=CC=C[CH]C2=C1CCNCC1=CC=C(\C=C\C(=O)NO)C=C1 FWZRWHZDXBDTFK-ZHACJKMWSA-N 0.000 claims 1
- MDYDGUOQFUQOGE-UHFFFAOYSA-N S-[7-oxo-7-[(4-phenyl-1,3-thiazol-2-yl)amino]heptyl] 2-methylpropanethioate Chemical compound S1C(NC(=O)CCCCCCSC(=O)C(C)C)=NC(C=2C=CC=CC=2)=C1 MDYDGUOQFUQOGE-UHFFFAOYSA-N 0.000 claims 1
- MFBOGIVSZKQAPD-UHFFFAOYSA-M Sodium butyrate Chemical compound [Na+].CCCC([O-])=O MFBOGIVSZKQAPD-UHFFFAOYSA-M 0.000 claims 1
- 241000282898 Sus scrofa Species 0.000 claims 1
- RTKIYFITIVXBLE-QEQCGCAPSA-N Trichostatin A Chemical compound ONC(=O)/C=C/C(/C)=C/[C@@H](C)C(=O)C1=CC=C(N(C)C)C=C1 RTKIYFITIVXBLE-QEQCGCAPSA-N 0.000 claims 1
- WAEXFXRVDQXREF-UHFFFAOYSA-N Vorinostat Chemical compound ONC(=O)CCCCCCC(=O)NC1=CC=CC=C1 WAEXFXRVDQXREF-UHFFFAOYSA-N 0.000 claims 1
- 229960000237 Vorinostat Drugs 0.000 claims 1
- NCNRHFGMJRPRSK-MDZDMXLPSA-N belinostat Chemical compound ONC(=O)\C=C\C1=CC=CC(S(=O)(=O)NC=2C=CC=CC=2)=C1 NCNRHFGMJRPRSK-MDZDMXLPSA-N 0.000 claims 1
- 229960003094 belinostat Drugs 0.000 claims 1
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- 210000000646 extraembryonic cell Anatomy 0.000 claims 1
- 235000012631 food intake Nutrition 0.000 claims 1
- 230000002068 genetic Effects 0.000 claims 1
- 210000000107 myocyte Anatomy 0.000 claims 1
- 229960005184 panobinostat Drugs 0.000 claims 1
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 1
- 210000001082 somatic cell Anatomy 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- WWGBHDIHIVGYLZ-UHFFFAOYSA-N tert-butyl N-[4-[3-[[7-(hydroxyamino)-7-oxoheptyl]carbamoyl]-1,2-oxazol-5-yl]phenyl]carbamate Chemical compound C1=CC(NC(=O)OC(C)(C)C)=CC=C1C1=CC(C(=O)NCCCCCCC(=O)NO)=NO1 WWGBHDIHIVGYLZ-UHFFFAOYSA-N 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
Claims (26)
- 家畜、家禽、狩猟動物又は水生動物種の自己複製細胞株を筋原性転写因子で修飾して、筋原性転写因子修飾細胞株を生成すること、及び
前記修飾細胞株を外因性調節により誘導し、ここで分化した修飾細胞株が筋細胞と筋管を形成すること、及び
前記筋細胞と筋管を培養して骨格筋線維を生成させ、これにより培養された食肉製品を生成すること、
を含む、培養された食肉製品をインビトロで生成する方法。 - 前記培養された食肉製品が、家畜又は家禽種のものである、請求項１に記載の方法。
- 前記培養された食肉製品が、家畜種のものである、請求項２に記載の方法。
- 前記家畜種が、ブタ又はウシである、請求項３に記載の方法。
- 前記生成された培養された食肉製品が、ヒト及び非ヒトの食物消費に適合される、請求項１〜４のいずれか１項に記載の方法。
- 前記自己複製細胞株が、胚性幹細胞株、人工多能性幹細胞株、胚体外細胞株、及び体細胞株からなる群から選択される、請求項１〜５のいずれか１項に記載の方法。
- 前記筋原性転写因子が、ＭＹＯＤ１、ＭＹＯＧ、ＭＹＦ５、ＭＹＦ６、ＰＡＸ３、ＰＡＸ７、それらのパラログ、オーソログ、及び遺伝子変異体からなる群から選択される、請求項１〜６のいずれか１項に記載の方法。
- 前記筋原性転写因子が、ＭＹＯＤ１である、請求項１〜７のいずれか１項に記載の方法。
- 未分化細胞ストック増殖のために第１の培地中に前記修飾細胞株を維持することをさらに含む、請求項１〜８のいずれか１項に記載の方法。
- 細胞ストック増殖のためにドキシサイクリンを含む第１の培地中に前記修飾細胞株を維持することをさらに含む、請求項９に記載の方法。
- 系譜特異的な分化のために第２の培地中に前記修飾細胞株を移し替えることをさらに含む、請求項１〜１０のいずれか１項に記載の方法。
- 系譜特異的な分化のためにＥ２を含む第２の培地中で前記修飾細胞株を処理することをさらに含む、請求項１１に記載の方法。
- 前記第２の培地が、古典的ＷＮＴシグナル伝達経路を活性化する試薬、エピジェネティックな調節のための試薬、又はそれらの組合わせをさらに含む、請求項１１又は１２に記載の方法。
- 前記第２の培地が、古典的ＷＮＴシグナル伝達経路を活性化する試薬をさらに含む、請求項１３に記載の方法。
- 前記古典的ＷＮＴシグナル伝達経路を活性化する試薬が、ＧＳＫ３β阻害剤を含む、請求項１４に記載の方法。
- 前記ＧＳＫ３β阻害剤が、ＣＨＩＲ９９０２１、塩化リチウム、ＢＩＯ、ＣＨＩＲ９９０２１、ＳＢ２１６７６３、ＣＨＩＲ−９８０１４、ＴＷＳ１１９、チデグルシブ、ＩＭ−１２、１−アザケンパウロン（1-Azakenpullone）、ＡＲ−Ａ０１４４１８、及びＳＢ４１５２８６からなる群の１つ又は複数の構成員から選択される、請求項１４に記載の方法。
- 前記ＧＳＫ３β阻害剤が、ＣＨＩＲ９９０２１である、請求項１６に記載の方法。
- 前記第２の培地が、エピジェネティックな調節のための試薬をさらに含む、請求項１３に記載の方法。
- 前記エピジェネティックな調節のための試薬が、５ＡＣ、ＲＧ１０８、スクリプタイド、酪酸ナトリウム、トリコスタチンＡ、スベロイルアニリドヒドロキサム酸、ＭＳ−２７５、ＣＩ−９９４、ＢＭＬ−２１０、Ｍ３４４、ＭＧＣＤ０１０３、ＰＸＤ１０１、ＬＢＨ−５８９、ツバスタチンＡ、ＮＳＣ３８２５、ＮＣＨ−５１、ＮＳＣ−３８５２、ＨＮＨＡ、ＢＭＬ−２８１、ＣＢＨＡ、サレルミド、ピメリン酸ジフェニルアミド、ＩＴＦ−２３５７、ＰＣＩ−２４７８１、ＡＰＨＡ化合物８、ドロキシノスタット（Droxinostat）、ＳＢ−９３９、ヒストンデアセチラーゼパラログ、ヒストンアセチルトランスフェラーゼパラログ、ｔｅｔ−メチシトシンジオキシゲナーゼパラログ（tet-methycytosine dioxygenase paralogs）、ヒストンデメチラーゼパラログ、ヒストンメチルトランスフェラーゼパラログ、及びＤＮＡメチルトランスフェラーゼパラログ、ヒストン、並びにＭｉ−２／ＮｕＲＤ及びＳＷＩ／ＳＮＦを含むクロマチンリモデリング複合体のサブユニットからなる群の１つ又は複数の構成員から選択される、請求項１８に記載の方法。
- 前記エピジェネティックな調節のための試薬が、ＤＮＡメチル化の阻害剤である、請求項１８又は１９に記載の方法。
- 前記筋組織が、骨格筋線維を含む、請求項１〜２０のいずれか１項に記載の方法。
- 前記古典的ＷＮＴシグナル伝達経路が、ＧＳＫ３β又はその基質ＣＴＮＮＢ１の遺伝子編集阻害により活性化される、請求項１〜２１のいずれか１項に記載の方法。
- 請求項１〜２２のいずれか１項に記載のインビトロでの方法により生成される培養された食肉製品。
- 前記エピジェネティックな調節のための試薬が、ヒストンデアセチラーゼパラログ、ヒストンアセチルトランスフェラーゼパラログ、ｔｅｔ−メチルシトシンジオキシゲナーゼパラログ、ヒストンデメチラーゼパラログ、ヒストンメチルトランスフェラーゼパラログ、及びＤＮＡメチルトランスフェラーゼパラログ、ヒストン、並びにＭｉ−２／ＮｕＲＤ及びＳＷＩ／ＳＮＦを含むクロマチンリモデリング複合体のサブユニットからなる群の１つ又は複数の構成員から選択される、請求項１８に記載の方法。
- 前記培地が１７−βエストラジオール（Ｅ２）を含む、請求項１２に記載の方法。
- 前記筋管が多核の筋管である、請求項１に記載の方法。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361962068P | 2013-10-30 | 2013-10-30 | |
| US61/962,068 | 2013-10-30 | ||
| PCT/US2014/063250 WO2015066377A1 (en) | 2013-10-30 | 2014-10-30 | Method for scalable skeletal muscle lineage specification and cultivation |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020005873A Division JP2020089381A (ja) | 2013-10-30 | 2020-01-17 | 拡張性のある骨格筋系譜形成及び培養の方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016535601A JP2016535601A (ja) | 2016-11-17 |
| JP2016535601A5 true JP2016535601A5 (ja) | 2017-12-07 |
| JP6728049B2 JP6728049B2 (ja) | 2020-07-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016552246A Active JP6728049B2 (ja) | 2013-10-30 | 2014-10-30 | 拡張性のある骨格筋系譜形成及び培養の方法 |
| JP2020005873A Pending JP2020089381A (ja) | 2013-10-30 | 2020-01-17 | 拡張性のある骨格筋系譜形成及び培養の方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
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| JP2020005873A Pending JP2020089381A (ja) | 2013-10-30 | 2020-01-17 | 拡張性のある骨格筋系譜形成及び培養の方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US20160251625A1 (ja) |
| EP (1) | EP3071040B2 (ja) |
| JP (2) | JP6728049B2 (ja) |
| CN (2) | CN105899079A (ja) |
| AU (2) | AU2014342180B2 (ja) |
| BR (1) | BR112016009803B1 (ja) |
| CA (1) | CA2928726A1 (ja) |
| ES (1) | ES2782380T5 (ja) |
| IL (2) | IL296889A (ja) |
| WO (1) | WO2015066377A1 (ja) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105899079A (zh) | 2013-10-30 | 2016-08-24 | 密苏里大学的策展人 | 可扩展性骨骼肌谱系特化和培育的方法 |
| US11318167B2 (en) | 2015-12-11 | 2022-05-03 | The Johns Hopkins University | Isolation of fusion-competent myoblasts and therapeutic applications thereof related to muscular dystrophy |
| WO2017124100A1 (en) * | 2016-01-14 | 2017-07-20 | Memphis Meats, Inc. | Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process |
| IL291373B2 (en) | 2016-07-11 | 2023-10-01 | Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd | Methods and systems for growing cells in culture |
| WO2018208628A1 (en) | 2017-05-06 | 2018-11-15 | Memphis Meats, Inc. | Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure |
| WO2018227016A1 (en) * | 2017-06-07 | 2018-12-13 | Wild Type, Inc. | Ex vivo meat production |
| EP3638777A4 (en) | 2017-07-13 | 2021-05-12 | Memphis Meats, Inc. | COMPOSITIONS AND METHODS FOR INCREASING THE EFFICIENCY OF CELL CULTURES FOR FOOD MANUFACTURING |
| GB2573327B (en) * | 2018-05-03 | 2021-05-12 | Mosa Meat Bv | Apparatus and process for production of tissue from cells |
| CA3201964C (en) | 2019-05-28 | 2023-10-31 | Upside Foods, Inc. | Apparatuses and methods for preparing a comestible meat product |
| CA3160109C (en) | 2019-11-20 | 2023-07-04 | Upside Foods, Inc. | Apparatuses and systems for preparing a meat product |
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