JP2016506811A - 光減衰媒体中の細胞への所望の光量の送達方法 - Google Patents
光減衰媒体中の細胞への所望の光量の送達方法 Download PDFInfo
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Abstract
Description
実質的に光減衰材料を含まない懸濁液について、リンパ球中のアポトーシス反応(所望の結果)に送達される関連する光量(この場合はUVA)の検量線の作成は以下のように決定された。
次に、ヘマトクリット(すなわち、光減衰材料の濃度)と関連させて検量線が生成され、産物の厚みを吸収された紫外光のパーセンテージに対する産物の厚みは以下のように測定された。
Claims (13)
- 全血を単核細胞を含む1以上の成分に分離するための処理室と、分離された単核細胞を受容するための、既知の体積の単核細胞懸濁液が受容された時に予め定められた厚みを有する少なくとも1つの処理容器とを有する使い捨て流体回路と、
前記処理室を受容して全血からの単核細胞の分離を発生させるのに適した分離装置と、
前記貯蔵容器を受容するのに適した照射装置と、
前記貯蔵容器内に受容された懸濁された単核細胞のヘマトクリットを制御するように構成された制御部とを備える、循環光療法システム。 - 収容された前記既知の体積の懸濁された単核細胞を含む前記処理容器が予め定められた約4mm〜5mmの厚みを有する、請求項1に記載の循環光療法システム。
- 前記処理容器は4.5mmの厚みを有する、請求項2に記載の循環光療法システム。
- 制御部は、2%〜3%のヘマトクリットを有する懸濁された単核細胞を提供するように構成されている、請求項2に記載の循環光療法システム。
- 制御部は、2.5%のヘマトクリットを有する懸濁された単核細胞を提供するように構成されている、請求項3に記載の循環光療法システム。
- a)生物学的流体を細胞産物を含む1以上の要素に分離するために分離室を備える使い捨て流体回路と、前記細胞産物を受容するのに適している少なくとも1つの処理容器とを得、前記流体回路は前記分離室と前記処理容器との間に無菌閉鎖経路を備えること、
b)前記分離室をアフェレーシス装置上に設置し、前記処理容器を照射装置上に設置し、前記アフェレーシス装置は前記回路を通して流体流通を生じさせるために少なくとも1つのポンプを含むこと、
c)生物学的流体の源からある体積の前記生物学的流体を前記分離室に導入し、前記細胞産物を前記体積の生物学的流体から前記分離室内で分離すること、
d)前記分離された細胞産物を、懸濁液を形成するように、選択された量の活性剤と結合させること、
e)前記懸濁液が予め定められたヘマトクリットと予め定められた厚みを有するように前記アフェレーシス装置の前記少なくとも1つのポンプの運動によって、分離された細胞産物の前記懸濁液と活性剤を前記処理容器内に導入すること、
f)分離された細胞産物と活性剤とが結合された前記懸濁液を前記照射装置内で光で処理すること、
g)前記アフェレーシス装置の前記少なくとも1つのポンプによって前記処理された細胞産物を前記処理室から引くこと、
のステップを含む体外循環光療法手順を実施するための方法。 - 分離された細胞産物は単核細胞を含み、懸濁液のヘマトクリットは2%〜3%であり、前記処理容器内の懸濁液の厚みは4mm〜5mmである、請求項6に記載の方法。
- 前記懸濁液のヘマトクリットは2.5%であり、前記処理容器内の前記懸濁液の厚みは4.5mmである、請求項7に記載の方法。
- 前記処理容器内の懸濁液の厚みは、既知の表面積を有する処理容器を提供することによって決定され、分離された細胞産物と活性剤とが結合された前記懸濁液の既知の体積を導入するように前記アフェレーシス装置の少なくとも1つのポンプを動作させる、請求項6に記載の方法。
- 懸濁液が既知の量の光エネルギーに曝されたときに所望の治療的効果が得られるように、標的細胞と、光エネルギー減衰物質と、光エネルギー活性化組成物を含む懸濁液を調製する方法であって、ステップは以下を含む:
標的細胞と光エネルギー活性化組成物を含み実質的に光エネルギー減衰物質を含まない複数の第1の試料を調製すること;
既知の量の光エネルギーをそれぞれの第1の試料に適用すること;
第1の試料の治療的反応を測定すること;
治療的反応に基づいて、標的細胞を含む懸濁液に送達された光エネルギーの量を予測できるように、適用された光量に対して第1の試料のそれぞれの治療的反応を比較すること;
標的細胞と、様々な量の光エネルギー減衰物質と、光エネルギー活性化組成物とを含む複数の第2の試料を調製すること;
既知の量の光エネルギーをそれぞれの第2の試料に適用すること;
第2の試料のそれぞれの治療的反応を測定すること;
治療的反応を得るためにそれぞれの第2の試料によって吸収された光エネルギーの量を決定するために、それぞれの第2の試料の治療的反応を、第1の試料の治療的反応と比較すること;
それぞれの第2の試料によって吸収された光エネルギーと、治療的反応に基づいてそれぞれの第2の試料に適用された光エネルギーと量との比を決定し、プロットの傾斜が実質的に平らになるように光エネルギー減衰物質の様々な量の範囲を決定するために、それぞれの第2の試料中の光エネルギー減衰物質の様々な量に対してそれぞれの第2の試料についてその比を比較すること;そして
光減衰物質の量が前記範囲内になるように処理される懸濁液を調製すること。 - 懸濁液が既知の量の光エネルギーに曝された時に所望の治療的効果を得るために、標的細胞と、光エネルギー減衰物質と、光エネルギー活性化組成物とを含む懸濁液中で、懸濁液中に存在し得る光エネルギー減衰物質の量の範囲を決定するための方法であり、ステップは以下を含む:
標的細胞と光エネルギー活性化組成物を含み実質的に光エネルギー減衰物質を含まない複数の第1の試料を調製すること;
既知の量の光エネルギーをそれぞれの第1の試料に適用すること;
第1の試料の治療的反応を測定すること;
治療的反応に基づいて、標的細胞を含む懸濁液に送達された光エネルギーの量を予測できるように、適用された光量に対して第1の試料のそれぞれの治療的反応を比較すること;
標的細胞と、様々な量の光エネルギー減衰物質と、光エネルギー活性化組成物とを含む複数の第2の試料を調製すること;
既知の量の光エネルギーをそれぞれの第2の試料に適用すること;
第2の試料のそれぞれの治療的反応を測定すること;
治療的反応を得るためにそれぞれの第2の試料によって吸収された光エネルギーの量を決定するために、それぞれの第2の試料の治療的反応を、第1の試料の治療的反応と比較すること;そして
それぞれの第2の試料によって吸収された光エネルギーと、治療的反応に基づいてそれぞれの第2の試料に適用された光エネルギーと量との比を決定し、プロットの傾斜が実質的に平らになるように光エネルギー減衰物質の様々な量の範囲を決定するために、それぞれの第2の試料中の光エネルギー減衰物質の様々な量に対してそれぞれの第2の試料についてその比を比較すること。 - 標的細胞は単核細胞を含み、光エネルギー減衰物質は赤血球と血漿を含み、光エネルギー活性化組成物はソラレンを含み、所望の治療的効果は予め定められたパーセンテージの単核細胞にアポトーシスを生じ、光エネルギー減衰物質の量は複数の第2の試料において、それぞれの第2の試料のヘマトクリットと厚みを変動することによって変動させられる、請求項10に記載の方法。.
- 標的細胞は単核細胞を含み、光エネルギー減衰物質は赤血球と血漿を含み、光エネルギー活性化組成物はソラレンを含み、所望の治療的効果は予め定められたパーセンテージの単核細胞にアポトーシスを生じ、光エネルギー減衰物質の量は複数の第2の試料において、それぞれの第2の試料のヘマトクリットと厚みを変動することによって変動させられる、請求項11に記載の方法。
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