JP2016202107A - Novel microorganisms having excellent antibacterial action and plant growth promoting action - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide Trichoderma microorganisms having excellent antibacterial action and plant growth promoting action, and microbiologic pesticides and plant growth promoters comprising a culture and/or bacterial cell thereof as an active ingredient.SOLUTION: The present invention provides a novel microorganism belonging to Trichoderma atroviride, trichoderma atroviride S/R-06 strain (accession number: NITE P-02029), and microbiologic pesticides and plant growth promoters comprising a culture and/or bacterial cell thereof as an active ingredient.SELECTED DRAWING: Figure 14

Description

  The present invention relates to a novel microorganism excellent in antibacterial action and plant growth promoting action, and in particular, a novel microorganism trichoderma atroviride S.R-06 belonging to Trichoderma atroviride and its culture and / or cell body The present invention relates to a microbial pesticide and a plant growth promoter as active ingredients.

  Trichoderma microorganisms are filamentous fungi that are widely found in soil, and have the property of hindering the growth of other fungi and the like and promoting the growth of plants, so they are mainly used as microbial preparations used in the agricultural field. Has been.

  Conventionally, preparations using Trichoderma microorganisms include, for example, plant disease control agents containing Trichoderma harzianum SK-55 as an active ingredient (Patent Document 1), Trichoderma viride T-3 strain, Trichoderma virensu T04398 strain, Trichoderma virens strain T04401, Trichoderma virens strain T04464 or mutants thereof as active ingredients, an agent for controlling pesticidal diseases in soybean (Patent Document 2), Trichoderma atroviride SKT-1 strain, Trichoderma atroviride SKT-2 strain A plant seed germination rate improver (Patent Document 3) containing at least one selected from Trichoderma atrobide SKT-3 strain as an active ingredient is disclosed.

Japanese Patent No. 3046167 Japanese Patent No. 5052873 JP 2006-124280 A

  However, the effects of these microbial preparations are not yet sufficient, and Trichoderma microorganisms having more excellent antibacterial action and plant growth promoting action have been demanded. Therefore, an object of the present invention is to provide a microorganism of the genus Trichoderma excellent in an antibacterial action and a plant growth promoting action, a microbial pesticide and a plant growth promoting agent containing the culture and / or fungus body as active ingredients.

  As a result of diligent research, the present inventors have found that a strain isolated from the soil of Shizunai-cho, Hokkaido Hidaka Branch Office is a novel microorganism belonging to Trichoderma atroviride, compared with a reference strain of a microorganism belonging to the genus Trichoderma. The inventors have found that the hyphal elongation rate is remarkably fast, exhibiting a high growth inhibitory effect on a wide variety of pathogens, and remarkably promoting the growth of plants, and have completed the following inventions.

(1) The novel microorganism according to the present invention is trichoderma atroviride S · R-06 strain (Accession Number: NITE P-02029).

(2) The microbial pesticide according to the present invention comprises, as an active ingredient, a culture and / or fungus body of trichoderma atroviride S · R-06 (accession number: NITE P-02029).

(3) The plant growth promoter according to the present invention comprises a culture and / or fungus body of trichoderma atroviride S · R-06 strain (Accession No .: NITE P-02029) as an active ingredient.

  According to trichoderma atroviride S · R-06 strain, its growth can be effectively suppressed against a wide variety of pathogens. Therefore, trichoderma atroviride S · R-06 strain can be used as an active ingredient of microbial pesticides for controlling plant diseases, and can also be used for the manufacture of a medicament for preventing or treating human animal diseases. .

  Further, according to trichoderma atroviride S · R-06 strain, it is possible to remarkably promote plant growth. Therefore, trichoderma atroviride S · R-06 strain can be used as an active ingredient of a plant growth promoter.

  Furthermore, since the microorganisms of the genus Trichoderma are commonly found in the soil, according to trichoderma atroviride S · R-06, the environmental load is low and the safety is high, such as microbial pesticides and plant growth promoters. Microbial preparations can be produced.

It is a table | surface which shows the top 30 with the high base sequence and the homology rate of the ITS area | region of SIID13819 strain as a result of the BLAST search in Apollon DB-FU (ITS) database Ver7.0. It is a table | surface which shows the top 30 which had a high homology rate with the base sequence of the ITS area | region of SIID13819 strain as a result of the BLAST search in an international base sequence database. It is a figure which shows the molecular phylogenetic tree created using the base sequence of the ITS area | region of SIID13819 strain, and the base sequence of the ITS area | region of the strain | stump | stock shown by * in FIG. It is a photograph which shows the colony of SIID13819 strain. It is a photograph which shows the vegetative mycelium of SIID13819 strain. It is a photograph which shows the conidia pattern of SIID13819 stock. It is a photograph which shows the conidia pattern of SIID13819 stock. It is a photograph which shows the conidia pattern of SIID13819 stock. It is a photograph which shows the conidia formation cell of SIID13819 strain | stump | stock. It is a photograph which shows the conidia formation cell of SIID13819 strain | stump | stock. It is a photograph which shows the conidia of SIID13819 stock. It is a photograph which shows the thick film spore of SIID13819 strain. It is the table | surface which shows the colony of SIID13819 stock | strain cultured on each temperature conditions of 15 degreeC, 20 degreeC, 25 degreeC, 30 degreeC, and 35 degreeC, and the colony diameter and the presence or absence of pigment production. It is a photograph which shows the result of having performed the opposing culture | cultivation with various pathogens and antagonistic bacteria (S * R-06 strain and 1017676 strain), and evaluating the growth inhibitory effect with respect to a pathogen. It is a photograph which shows the result of having performed the opposing culture | cultivation with various pathogens and antagonistic bacteria (S * R-06 strain and 1017676 strain), and evaluating the growth inhibitory effect with respect to a pathogen.

  Hereinafter, the trichoderma atroviride S · R-06 strain and the microbial pesticide and plant growth promoter according to the present invention will be described in detail. trichoderma atroviride S • R-06 strain (hereinafter sometimes simply referred to as “S • R-06 strain”) was incorporated on March 27, 2015, as shown in Example 1 described later. Since it was deposited as the NITE P-02029 with the Deposit Number NITE P-02029 at the Product Evaluation Technology Base Organization Patent Microorganism Deposit Center (2-5-8 Kazusa-Kamashita, Kisarazu City, Chiba Prefecture), it can be obtained from this institution.

  The S · R-06 strain can be cultured or grown by stationary culture at 20-30 ° C. for several days in an agar medium. Examples of the agar medium include Potato Sucrose Agar (PSA medium), Corneal Dextrose Agar (CMD medium; Samuels et. Al., 1998), Potato dextrose agar medium (PDA medium; “Digo” Nippon Pharmaceutical Co., Ltd.), Synthetischerch. agar (SNA medium; Nirenberg, 1976) can be used.

  Here, the PSA medium can be prepared by the following procedure. First, potatoes are washed with tap water, then peeled, cut into 1 cm squares, and quickly washed with tap water. Subsequently, boil the potatoes (1 cm squares that have been peeled and cut) with 200 gram of 1 L of distilled water for 20 minutes. After crushing it, it is squeezed into a cotton bag and the liquid is recovered. Here, 20 g of sucrose is added and dissolved. After adjusting the pH to 5.6, 20 g of agar is added, and distilled water is added so that the final volume is 1 L. This is subjected to autoclaving (high pressure steam sterilizer) and sterilized at 121 ° C. for 15 minutes.

  The S · R-06 strain can also be cultured or grown by shaking culture in a liquid medium at 20 to 30 ° C. for several days. Examples of the liquid medium include potato-dextrose liquid medium (DIFCO), malt extract medium (malt extract 20 g, peptone 1 g, glucose 20 g, distilled water 1000 ml) and glucose bouillon medium (meat extract 10 g, NaCl 5 g, peptone 10 g, For example, 10 g of glucose and 1000 ml of distilled water) can be used.

  Since S · R-06 strain is excellent in antibacterial action and plant growth promoting action as shown in the examples described later, its culture and fungus are mainly used in the agricultural field such as microbial pesticides and plant growth promoting agents. It can be used as an active ingredient of a microbial preparation used in 1. That is, the microbial pesticide and the plant growth promoter according to the present invention contain the culture and / or fungus body of S · R-06 strain as active ingredients.

The procedure for producing the microbial preparation (microbial pesticide, plant growth promoter) according to the present invention is not particularly limited. For example, it can be produced by the following procedure. First, an equal amount of water is added to the bran and sterilized by heating at 120 ° C. to prepare a bran medium. After cooling this to room temperature, the S · R-06 strain cultured or propagated by the above-mentioned method is added as an inoculum. Subsequently, the bran medium to which the S · R-06 strain has been added is placed flat on a tray to a depth of 5 cm or less and cultured at 25 ° C. for 7 days. Thereafter, drying is performed with dehumidified air at 40 ° C. or lower until the water content becomes 8% or lower. Then, it grind | pulverizes to the particle size of 80 mesh pass, and uses the obtained powder as a microbial formulation (a microbial pesticide, a plant growth promoter). Usually, 1 × 10 ⁸ to 1 × 10 ¹¹ conidia are contained in 1 g of the powdered microbial preparation produced by this procedure.

  As a procedure for producing a microbial preparation (microbicide pesticide, plant growth promoter) according to the present invention, distilled water obtained by culturing S · R-06 strain on the agar medium and sterilizing the formed conidia is also available. It is good also as a liquid microorganism preparation (microbicide pesticide, plant growth promoter).

Next, the application procedure of the microbial preparation (microbial pesticide, plant growth promoter) according to the present invention is also not particularly limited, and for example, it can be applied by the following procedure. That is, if it is a powdery microbial preparation, a required amount is suspended in water, and if it is a liquid microbial preparation, it is sprayed as it is on the soil for growing the target plant. Here, the required amount of the microbial preparation according to the present invention is often a small amount, it is difficult to spread uniformly, so if necessary, diluted with water, soil, fertilizer, soil improvement material, etc., It is preferable to spray after increasing the total amount. When the solid is diluted or increased, it is preferable to spray the microorganism preparation according to the present invention and then spray water to promote the establishment of the S · R-06 strain on the soil. The required amount of the microorganism preparation according to the present invention is 3 to 5 g per 1 m ^{2 of} soil in the case of powder, and 1 × 10 ⁹ conidia per 1 m ^{2 of} soil in the case of liquid. Can be.

  As an application procedure of the microbial preparation (microbial pesticide, plant growth promoter) according to the present invention, it is applied by mixing with seeds of the target plant instead of spraying on the soil and sowing the seeds. May be.

  The Trichoderma atroviride S · R-06 strain and the microbial pesticide and plant growth promoter according to the present invention will be described below based on examples. Note that the technical scope of the present invention is not limited to the features shown by these examples.

<Example 1> Isolation and identification of strains (1) Isolation of strains From the soil of Shizunai-cho, Hokkaido Hidaka branch office, a fungus having the form of Astragalus was isolated and used as SIID13819 strain.

(2) Identification using ITS region base sequence Entrusted to Techno Suruga Labs Co., Ltd., analyzed the base sequence of the internal transcription spacer region (ITS region) of SIID13819 strain, and identified the attribution. went. Specifically, analysis was performed using the following reagents, devices, and programs.

DNA extraction; physical disruption and modification of Marmur (1961) PCR; PrimeSTAR HS DNA Polymerase (Takara Bio)
Cycle sequence; BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems)
Sequencing; ChromaPro 1.5 (Technology Pty Ltd.)
Analysis software: Apollon 2.0 (Techno Suruga Lab)
Homology search; Basic Local Alignment Search Tool (BLAST) (NCBI http://www.ncbi.nlm.nih.gov/BLAST/)
Search database; Apollon DB-FU (ITS) database Ver 7.0 (Techno Suruga Lab); International base sequence database (GenBnk / DDBJ / EMBL)

  As a result of BLAST search, the top 30 having the highest homology with the nucleotide sequence (SEQ ID NO: 1) of the ITS region of SIID13819 strain are shown in FIG. 1 (Apollon DB-FU (ITS) database Ver7.0) and FIG. 2 (international nucleotide sequence). Database). In addition, FIG. 3 shows a molecular phylogenetic tree prepared using the base sequence (SEQ ID NO: 1) of the ITS region of the SIID13819 strain and the base sequence of the ITS region of the strain indicated by * in FIG.

  As shown in FIGS. 1 to 3, the base sequence (SEQ ID NO: 1) of the ITS region of SIID13819 strain is 100% identical to that of Hyporea atroviridis and Trichoderma atroviride and belongs to the same taxonomic group as these species. did. Hypocrea atroviridi and Trichoderma atrovide have a relationship between a teleomorph (sexual age) and an anamorph (asexual era).

(3) Identification by morphological observation The SIID13819 strain was identified as Corneal Dextrose Agar medium (Samuels et. Al., 1998; hereinafter referred to as “CMD medium”), potato dextrose agar medium (Digo; Nippon Pharmaceutical Co., Ltd .; hereinafter referred to as “PDA medium”) ) And Synthetischer nahrstoffarmer agar (Nirenberg, 1976; hereinafter referred to as “SNA medium”), the cells were cultured at 25 ° C. for 7 days, and morphological observation was performed using an optical microscope (BX51; Olympus). In addition, the lactophenol liquid and the lactophenol cotton blue liquid were used for the mount liquid. The observation result of the colony is shown in FIG. 4, and the observation result of the vegetative mycelium is shown in FIG. In addition, as observation results of asexual reproductive organs, conidia pattern observation results are shown in FIGS. 6 to 8, conidia-forming cell observation results are shown in FIGS. 9 and 10, and conidia observation results are shown in FIG. The spore observation results are shown in FIG. In addition, as a result of culturing SIID13819 strain for about 4 weeks under the same conditions, formation of a sexual reproductive organ was not recognized, and it was revealed that it was an asexual age.

  As shown in FIG. 4, in any of the CMD medium, the PDA medium, and the SNA medium, the colony diameter was 65 to 70 mm, and the surface property was velvet. The color of the colony is dark green (Deep green; 27E-8) and green (Green; 27B-8) to white (White; 27A-1) in CMD medium and PDA medium, and green and yellow in SNA. It was green (Light green; 27A-5) to white (White; 27A-1). Here, the numerical value in () indicates the code number of “color” used in Kornerup and Wancher (1978). In any medium, production of soluble pigment was not observed.

  Moreover, as shown in FIG. 5, the vegetative mycelium was formed on the agar surface or in the agar. The mycelium was thin or slightly thick and swollen, the width of the mycelium was 1.5 to 5 μm, and the color of the mycelium was colorless. In addition, the formation of septal hyphae was observed.

  In addition, as shown in FIGS. 6 and 7, the conidia pattern was upright from the vegetative mycelium and branched regularly or irregularly. Moreover, as shown in FIG. 8, it was recognized that the conidia pattern gathered and formed the lump (tuft) on wool.

  Moreover, as shown in FIGS. 9 and 10, the shape of the phialide, which is a conidia-forming cell, is an ampoule (spindle shape) with a thin tip, and the size is 6-10 μm long axis × 1.8-3 μm short axis. It was observed that it was formed at the tip of the conidial pattern.

  Moreover, as shown in FIG. 11, the conidia were fiaro type and the color tone was colorless to green. The shape was spherical to subspherical, and was 1 cell. The size was 2.5 to 3.5 μm long axis × 2 to 3 μm short axis, and the surface was smooth.

  Further, as shown in FIG. 12, thick spore was formed at the tip or middle of the vegetative mycelium, the shape was spherical to subspherical, the color tone was colorless, and it was unicellular.

  The morphological characteristics observed in the above Example 1 (3) almost coincided with the characteristics of Trichoderma atrobilide (Dodd et al., 2003, Trichoderma Online).

  From the results of the above Examples 1 (2) and (3), it was revealed that the SIID13819 strain belongs to the Ascomycota hunta molda button bamboo subclass button bamboo order button bamboo family Trichoderma atroviride (Trichoderma atroviride).

(4) Growth temperature test SID13819 strain was inoculated into PDA medium, cultured under each temperature condition of 15 ° C, 20 ° C, 25 ° C, 30 ° C and 35 ° C, and colony diameter measurement and pigment production on the third day of culture Observation was made for the presence or absence of. The result is shown in FIG.

  As shown in FIG. 13, colonies grew at 15 ° C., 20 ° C., 25 ° C. and 30 ° C., but no colony growth was observed at 35 ° C. The colony diameter was 30 to 32 mm at 15 ° C, 55 mm at 20 ° C, 60 mm at 25 ° C, and 50 to 55 mm at 30 ° C. Pigment production was not observed under any temperature conditions of 15 ° C, 20 ° C, 25 ° C, 30 ° C and 35 ° C. That is, the SIID13819 strain was different from the characteristics of Trichoderma atrobilide (Dodd et al., 2003, Trichoderma Online) in that no colony growth was observed at 35 ° C. From these results and the results of Examples 1 (2) and (3), it was revealed that SIID13819 strain is a novel strain belonging to Trichoderma atrobide.

  Therefore, the SIID13819 strain was named trichoderma atroviride S · R-06, and on March 27, 2015, the National Institute of Technology and Evaluation of the National Institute of Technology and Evaluation, 2-5-8 Kazusa Kamashichi, Kisarazu City, Chiba Prefecture ) As deposit number NITE P-02029. Hereinafter, trichoderma atroviride S • R-06 may be abbreviated as “S • R-06 strain”.

<Example 2> Evaluation of mycelial elongation rate Comparison of mycelial elongation rate between S.R-06 strain and Hyporea atroviridis NBRC 101776 (hereinafter referred to as "101767 strain"), which is a reference strain of Trichoderma atroviride sexual age. Went. Specifically, the S · R-06 strain and the 101776 strain were planarly cultured in a PDA medium at 27 ° C. for 3 days. Subsequently, the tip of the colony was punched out with a 6 mm diameter cork borer, placed in a PDA medium, and cultured at 27 ° C. for 2 days. The colony radius was measured on the first day and the second day of culture, and the value obtained by subtracting the colony radius on the first day from the colony radius on the second day of culture was defined as the hyphal elongation rate (mm / day) per day. The same experiment was performed 5 times, and the average value was calculated. The results are shown in Table 1.

  As shown in Table 1, the average value of the hyphal elongation rate was 21.4 mm / day for the S · R-06 strain and 18.0 mm / day for the 101767 strain. That is, the hyphal elongation rate of the S · R-06 strain was nearly 20% higher than the hyphal elongation rate of the 101767 strain. From this result, it was revealed that trichoderma atroviride S · R-06 has a significantly faster hyphal elongation than the reference strain of Trichoderma microorganism. Here, the hyphal elongation rate is an important factor in suppressing the growth of pathogens, and it is considered that the higher the hyphal elongation rate, the higher the ability to suppress the growth of pathogens and the better the antibacterial action. From this, it was considered that trichoderma atroviride S · R-06 has a higher ability to suppress the growth of pathogens than the reference strain of Trichoderma microorganisms and is excellent in antibacterial action.

<Example 3> Evaluation of antibacterial action The pathogens and antagonists shown in Table 2 were prepared, and were subjected to planar culture in PDA medium at the temperatures shown in Table 2 and the number of culture days.

Subsequently, colonies of each pathogen and antagonistic bacteria cultured in a plane were punched with a 6 mm diameter cork borer, each pathogen was placed 30 mm from the end of a 90 mm diameter petri dish containing PDA medium, and 30 mm from the other end. The antagonistic bacteria were placed in and cultured at 27 ° C. for 5 days. In addition, about Rhizopus chinensis NBRC 31988 (pathogen of rice seedling blight), only the opposing culture | cultivation with S * R-06 strain | stump | stock was performed as an antagonist. As a control, the one in which only the pathogen was placed was cultured in the same manner. Then, the area (pathogen growth area) where the pathogen was growing in each petri dish was measured, and the pathogen growth inhibition rate (%) was calculated using Equation 1. The results are shown in FIG. 14 and FIG.
Formula 1: pathogen growth inhibition rate (%) = {(pathogen growth area in control−pathogen growth area in each petri dish) / pathogen growth area in control} × 100

  As shown in FIG. 14, the pathogen growth inhibition rate against Fusarium oxysporum NBRC 7152 (pathogen causing plant wilt and rot) was 80% in the S · R-06 strain, while 64 in the 101769 strain. %Met. Also, Rhizoctonia solani NBRC 30944 (pathogen of Rhizoctonia disease), Sclerotinia sclerotiorum NBRC 30965 (pathogen of mycorrhizal disease) and Colletotrichum acutum NBRC 32850 (Role of anthracnose pathogens S All of the strains were 100%.

  Further, as shown in FIG. 15, the pathogen growth inhibition rate against Helicobasidium mompa NBRC 31651 (purple mompa disease pathogen) was 89% in the S · R-06 strain and 69% in the 101676 strain. there were. Moreover, the pathogen growth suppression rate with respect to Rosellia necatrix NBRC 32538 (pathogen of white Mompa disease) was 100% in the S.R-06 strain, and 87% in the 101767 strain. Moreover, the pathogen growth suppression rate with respect to Armillaria mellea NBRC31621 (pathogen of Nararatake disease) was 100% in both S · R-06 and 101776 strains. In addition, the pathogen growth inhibition rate against Athelia rolfsiii NBRC 31215 (white silkworm pathogen) was 74% in the S · R-06 strain and 70% in the 101767 strain. Moreover, the pathogen growth inhibition rate of the S.R-06 strain against Rhizopus chinensis NBRC 31988 (pathogen of rice seedling blight) was 100%.

  That is, S.R-06 strains are Rhizoctonia solani NBRC 30944 (pathogen of Rhizoctonia disease), Sclerotinia sclerotiorum NBRC 30965 (pathogen of myconuclear disease) and Colletotrichum actinum NBRC3A. Disease pathogens), 100% pathogen growth inhibition rate was exhibited in the same manner as 101776 strain, Fusarium oxysporum NBRC 7152 (pathogen causing plant wilt and spoilage), Helicobasidium mompa NBRC 31651 (pathogen of purple Mompa disease) ), Rossellinia necatrix NBRC 32538 (White Mon For disease pathogens) and Athelia rolfsii NBRC 31215 (pathogen Southern Blight), showed higher pathogen growth inhibition rate than 101776 strain.

  That is, trichoderma atroviride S · R-06 exhibits a high growth inhibitory effect against a wide variety of pathogens as compared with the reference strain of Trichoderma microorganisms, and thus has an excellent antibacterial action. It was.

<Example 4> Production and application of microbial pesticides (1) Production of microbial pesticides S · R-06 strain was shake-cultured in a liquid medium to prepare an inoculum. On the other hand, 10 kg of bran, 10 L of water, and 50 mL of 36% concentrated hydrochloric acid were mixed and sterilized by heating at 120 ° C. for 30 minutes in a trommel type sterilization pot. Inoculation was carried out by adding 100 mL of the inoculum of the prepared S · R-06 strain. An equal amount of each was placed in 20 aluminum culture trays (450 × 700 × 80 mm) and cultured for 7 days under conditions of 25 ± 1 ° C. and relative humidity of 90%. After the 4th day from the start of the culture, a stirring operation was performed once a day to promote conidia formation. After completion of the culture, the product was dried for about 12 hours under dehumidified air heated to 35 ° C. until the water content became 4% or less, to obtain 7.3 kg of a dried product. The obtained dried product was subjected to an impact pulverizer and pulverized to a particle size of 80 mesh pass, and the obtained dry powder was used as a microbial pesticide. In 1 g of this dry powder, about 8 × 10 ⁹ conidia were contained.

(2) Application of microbial pesticide The microbial pesticide of Example 4 (1) was diluted to a ratio of 1.5 g per liter of water. This was sprayed at a rate of ² L / m ^{2 on} turf grass of a golf course where brown patches with Rhizoctonia solani as a pathogen were generated, and the progress was observed. As a result, the progression of symptom stopped after 2 days, and the state recovered completely after 12 days. From this result, it became clear that the microbial pesticide containing trichoderma atroviride S · R-06 as an active ingredient can effectively control plant diseases.

<Example 5> Evaluation of plant growth promoting action (1) Production of plant growth promoting agent S / R-06 strain was applied to PDA medium and cultured at 25 ° C for 10 days to form conidia. Subsequently, conidia were suspended in sterilized distilled water to prepare a conidia solution, which was used as a plant growth promoter.

(2) Evaluation of plant growth promoting action 7 L of red clay, 2 L of humus, 1 L of permiculite, 15 g of lime and 15 g of chemical fertilizer were mixed to prepare a soil for cultivation, put in a planter, and a test zone and a control zone were set. The plant growth promoter of Example 5 (1) was sprayed on the test plot so that the sprayed amount would be 1 × 10 ⁹ conidia per 1 m ² . Nothing was sprayed on the control. Subsequently, Komatsuna seeds were sown in the test group and the control group, and cultivated under the same conditions for 28 days. Thereafter, 10 komatsuna strains were randomly collected from each ward, the plant height was measured, and the average value was calculated.

  As a result, the average plant height in the control group was 16.8 cm, whereas the average plant height in the test group was 20.5 cm. That is, the plant height of Komatsuna was remarkably large in the group sprayed with the plant growth promoter according to the present invention compared to the group not sprayed. From these results, it has been clarified that the plant growth promoter containing trichoderma atroviride S · R-06 as an active ingredient significantly promotes the growth of plants.

Claims (3)

  trichoderma atroviride S • R-06 strain (Accession Number: NITE P-02029).   A microbial pesticide comprising, as an active ingredient, a culture and / or fungus body of trichoderma atroviride S · R-06 strain (Accession Number: NITE P-02029).   A plant growth promoter comprising, as an active ingredient, a culture and / or fungus body of trichoderma atroviride S · R-06 strain (Accession Number: NITE P-02029).