JP2016121113A - Cosmetics for prevention or improvement of gray hair - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide hair cosmetics and cosmetics for improving gray hair having excellent stability and safety, while having excellent effect for preventing or improving gray hair development.SOLUTION: The invention provides a gray hair preventive agent containing Lilium maculatum extract or gray hair improvement cosmetics containing the gray hair preventive agent by finding out that gray hair preventive (gray hair blackening) effect together with excellent tyrosinase activity-promoting effect and melanin production-promoting effect are recognized in the extract of Lilium maculatum belonging to Liliaceae Lilium.SELECTED DRAWING: None

Description

The present invention relates to a tyrosinase activity promoter, a melanin production promoter, a gray hair preventing or gray hair improving cosmetic.

White hair is one of the physiological aging phenomena caused by changes in hair matrix pigment cells. Possible causes of gray hair include reduction in the number of melanocytes (pigmenting cells), decrease in melanogenesis enzymes, and disturbance of melanin pigment granule migration from melanocytes to keratinocytes. Is currently unknown.

There have been many reports on ingredients that prevent or improve white hair as methods for changing whitened hair to black hair, but none of them has been obtained in terms of effectiveness and safety. First, it is mainly about hair dyeing with hair dyes.

However, since hair dyeing with hair dyes is very damaging to the hair and the background, there is an extremely high demand for prevention of white hair by methods other than hair dyeing and to improve the resulting white hair as much as possible. The appearance of effective and inexpensive white hair improving cosmetics is eagerly desired.

The color of the hair is determined by the amount of pigment melanin in the hair. Melanin is biosynthesized from tyrosine by the enzyme tyrosinase in pigment cells (melanocytes) present in the hair bulb of skin and hair. Patent Documents 1, 2, 3 and Non-Patent Document 1 report that human hair can be blackened by activation of melanocytes or tyrosinase.

For this reason, in order to achieve the purpose of preventing white hair and improving white hair, it is an effective means to examine tyrosinase activity promoting components and melanin production promoting components effective in the mechanism of melanin pigment formation.
Therefore, if a component that increases the amount of melanin by directly acting on melanocytes and increasing their proliferation or promoting the activity of tyrosinase is found, the body's defense ability inherent to melanin is promoted and white hair is prevented. Or improvements can be realized.

So far, various proposals have been made regarding tyrosinase activity promoters and melanin production promoters. For example, Patent Document 4 proposes a tyrosinase activity promoter containing a dihydrolpeol derivative. In Patent Document 5, a melanin production promoter and a tyrosinase activity promoter containing pepper leaf or an extract thereof have been proposed, but in a situation where sufficient in terms of effectiveness and safety have not yet been obtained. is there.

Traditionally, liliaceous plants have been recognized as whitening agents. In patent document 6, the whitening cosmetic composition characterized by containing a lily family plant (Liliaceae) as an active ingredient is proposed. Patent Documents 7 and 8 have proposed as a whitening agent characterized by containing a plant belonging to the genus Lily family such as Madonna lily (Lilium Candidum) and Casablanca (Lilium Casa Blanca).

On the other hand, Patent Document 9 proposes Lilium macculatum Thunb., A lily family, as a hyaluronidase activity inhibitor and a moisturizing cosmetic. In Patent Document 10, as an example of cosmetics that can prevent rough skin and wrinkles, yellowing of hair, split ends, tears, etc., and improve the skin and hair from the essence, the lily family crude drug “Yuri” is used as a material. Is disclosed. However, the effect of reducing the occurrence of gray hair has been confirmed only with carrots, and no effect on the lily has been confirmed.

Thus, it has not been known so far that squid has a tyrosinase activity promoting effect and a melanin production promoting effect, and it has not been known that squid has a gray hair improving effect.

JP 2001-288052 A JP 2001-288098 A Japanese Patent Laid-Open No. 2007-8888 Japanese Patent Laid-Open No. 2002-3381 JP 2003-226649 A JP-A-5-345718 JP2013-203725A JP 2011-225564 A JP 2003-012489 A JP 2003-055139 A

Takashi Yoshida, “Anti-Aging Series 1 Actual Hair and Hair Removal”, p49-61, NTS (2005)

An object of the present invention is to provide a white hair preventing or white hair improving cosmetic that is excellent in preventing or improving the occurrence of white hair and having excellent safety.

As a result of intensive studies for solving the above-mentioned problems, the present inventors have found that an extract of a lily belonging to the genus Lily genus has an excellent tyrosinase activity promoting effect and melanin production promoting effect, as well as preventing and improving white hair. That is, the present invention provides a tyrosinase activity promoter, a melanin production promoter, a gray hair preventing or a gray hair improving cosmetic containing an extract of a lily belonging to the genus Lily family.

The extract of the lily belonging to the genus Lily family of the present invention exhibits excellent tyrosinase activity effect, melanin production promoting effect, prevention of white hair generation and improvement effect.

Lily macculatum Thunb. Used in the present invention is a plant belonging to the genus Lily family, and the alias is called Iwaturi, Iwayuri, Miyakashi Yuri, Yamakashi Yuri, Hamari. The part to be used is not particularly limited, and flowers, leaves, stems, buds, shoots, etc., which are above-ground parts, bulbs, roots, etc., which are underground parts, can be used in addition to whole grass, but bulbs are particularly preferable. The bulb described in the present invention refers to a shortened stem with an enlarged leaf or part of a leaf (scale leaf) overlapping, and a new shoot refers to a newly emerged bud (young shoot). Refers to the entire region above the bulb.

Although the preparation method of a squash lily extract is not specifically limited, For example, it extracts from under low temperature to warm using various suitable organic solvents. Examples of the extraction solvent include water; lower monohydric alcohols such as methyl alcohol and ethyl alcohol; liquid polyhydric alcohols such as glycerin, propylene glycol, and 1,3-butylene glycol; ketones such as acetone and methyl ethyl ketone; and ethyl acetate. One type or two or more types of alkyl esters; hydrocarbons such as benzene and hexane; ethers such as diethyl ether; halogenated alkanes such as dichloromethane and chloroform can be used. Among them, water, ethyl alcohol, and a mixed solvent of one or more of 1,3-butylene glycol are particularly suitable.

Although the extraction method of a squash lily extract is not specifically limited, For example, if it is dried, it is 1 to 1000 times by weight, particularly 10 to 100 times by weight of solvent, and is 0 ° C. or more, particularly 20 ° C. to 40 ° C. It is preferably performed for 1 hour or longer, particularly 3 to 7 days. Moreover, you may heat-extract at 60-100 degreeC for 1 hour.

The squash lily extract obtained under the above conditions may be used as an extracted solution, but if necessary, it may be used by appropriately filtering and concentrating it after filtration or the like. it can.

In the case where the present invention is used as a gray hair preventing or gray hair improving cosmetic, an effective amount of the squash lily extract may be present. In general, it is preferably 0.0001 to 100% by mass in terms of evaporated and dried content for the composition for preventing or improving white hair, and the range of 0.01 to 10.0% by mass is particularly optimal. .

The white hair prevention or white hair improving cosmetic of the present invention is within the range not impairing the effects of the present invention, for example, fats and oils, waxes, hydrocarbon oils, ester oils, higher alcohols, silicone oils, ultraviolet absorbers, ultraviolet scattering agents, Moisturizer, surfactant, water-soluble polymer, thickener, powder, various active ingredients, skin protectant, anti-aging agent, plant extract, antiseptic, anti-inflammatory agent, pH adjuster, sequestering agent, An antioxidant etc. can be mix | blended.

Examples of the dosage form of the cosmetic for preventing or improving white hair according to the present invention include, for example, ointments, creams, emulsions, lotions, funny, essences, packs, gels, hair mists, hair tonics, hair liquids, liniments, hair rinses, hair shampoos, hairs. Any treatments, hair conditioners, aerosols, mousses, etc. may be used as long as they are applicable to the hair or scalp, and the dosage form is not particularly limited.

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to these examples. Unless otherwise specified, the blending amount is expressed in mass%.

<Preparation of squash lily extract>
Squash lily extract was prepared using dried lily of root lily (root, bulb, above-ground part). 10 g of a 50 (W / V)% ethanol aqueous solution was added to 1 g of the plant raw material, followed by extraction by heating at 60 ° C. for 3 hours. After extraction, the mixture was filtered to remove the plant active ingredient, and then distilled off under reduced pressure to obtain a solid extract as a squash extract.

<Measurement of cell growth rate>
Human epidermal keratinocyte cells (HaCaT cells) 5.0 × 10 ⁴ cells / mL are dispersed in Dulbecco's modified Eagle medium containing 10% FBS, seeded in 0.5 mL each in a 24-well plate, and at 37 ° C. under 5% CO ₂ . Cultured for 24 hours. After 24 hours, a lily (bulb) extract (dry residue) was added to a concentration of 10 ppm and 100 ppm. 72 hours after cell seeding, WST assay was performed using Cell Counting Kit-8 (Dojindo) to evaluate cell proliferation rate. The results are shown in Table 1.

From the results in Table 1, no cytotoxicity was observed when the lily extract (100 ppm, 200 ppm) was added.

<Measurement of tyrosinase activity>
McLuvine buffer solution (pH 6.8) 1.0 mL, L-tyrosine aqueous solution (0.3 mg / 1.0 mL) 1.0 mL, and purified water 0.9 mL were mixed. 180 μL of this mixed solution was added to a 96-well plate, and the squash extract was added to the final concentrations shown in Table 2, and the absorbance at 475 nm and 655 nm ( _{A475 nm} , _{A655 nm} ) was measured. After measuring the absorbance, 20 μL of tyrosinase solution (manufactured by Sigma, derived from mushrooms, 0.5 mg / mL) was added, and after reacting at 37 ° C. for 15 minutes, absorbance at 475 nm and 655 nm ( _{B475 nm} , _{B655 nm} ) was measured. The acceleration rate of tyrosinase activity was calculated from the obtained absorbance according to the following formula, and the results are shown in Table 2.
<Calculation formula of tyrosinase activity promotion rate>
(1) Tyrosinase activity amount = ( _{B475 nm} − _{B655 nm} ) − ( _{A475 nm− A655 nm} )
(2) Tyrosinase activity promotion rate (%) = {(SB) / (CB)} × 100
S: Tyrosinase activity of sample (with tyrosinase added) B: Tyrosinase activity of blank (without tyrosinase added) C: Tyrosinase activity of control (distilled water instead of sample)

From the results in Table 2, it was confirmed that the scouring lily extract has an effect of promoting tyrosinase activity regardless of the site of use, but the effect of bulbs was particularly high.
In the above examples, the results of the extraction with 50 (W / V)% aqueous ethanol solution were shown for the roots, bulbs and above-ground part, but the same tendency was confirmed in other parts and extraction solvents.

<Measurement of intracellular melanin content>
Mouse melanoma cells (B16 melanoma cells) 5.0 × 10 ⁴ cells / mL were dispersed in Dulbecco's modified Eagle medium containing 10% FBS, seeded in 1 mL in a 12-well plate, and cultured at 37 ° C. under 5% CO ₂ . After 24 hours, a squash lily extract (dry residue) was added to a concentration of 100 ppm. After culturing for 96 hours after cell seeding, the medium was discarded and washed with PBS (−), and 500 μL of 2N NaOH was added. It was boiled at 100 ° C. for 30 minutes and centrifuged at 3500 rpm for 1 minute. 350 μL of this supernatant was transferred to a 96-well plate, and the absorbance at ₄₀₅ nm and 655 nm (A _{405 nm} , A _{655 nm} ) was measured. Furthermore, 40 μL of this supernatant was transferred to a 96-well plate, and the absorbance was measured at ₅₄₀ nm and 655 nm (B _{540 nm} , B _{655 nm} ) according to the BCA method to measure the amount of protein. Melanin production promotion rate was calculated from the obtained absorbance according to the following formula, and the results are shown in Table 3.

<Promoting rate of melanin production>
(1) Melanin amount per protein = (A _{405 nm-} B _{655 nm} ) / (B _{540 nm-} B _{655 nm} )
(2) Melanin production promotion rate (%) = S / C × 100
S: Amount of melanin per protein of sample (extracted extract) C: Amount of melanin per protein of control (no extract added)

From the results of Table 3, it was confirmed that the extract of squid lily has a melanin production promoting effect.
In addition, although the result in the extract in 50 (W / V)% ethanol aqueous solution was shown about the bulb in the said Example, the same tendency was confirmed also in the other site | part and the extraction solvent.

Hereinafter, prescription examples of the white hair preventing or white hair improving cosmetic according to the present invention will be shown. In addition, content is mass%. A manufacturing method is a conventional method.

<Prescription Example 1> Lotion 1,3-butylene glycol 10.00
Glycerin 2.00
Polyoxyethylene sorbitan monolaurate (20E.O.) 0.15
Ethanol 5.00
Squash lily extract (bulb; dry residue) 0.08
Preservative appropriate amount
Purified water balance <br/> Total 100

<Prescription Example 2> Emulsion squash lily extract (bulb; dry residue) 0.07
Squalane 8.0
Vaseline 2.0
Beeslow 0.5
Sorbitan sesquioleate 0.8
Polyoxyethylene oleyl ether (20E.0.) 1.2
Carboxyvinyl polymer 0.2
Propylene glycol 0.5
Potassium hydroxide 0.1
Ethanol 7.0
Preservative / Antioxidant Appropriate amount Fragrance Appropriate amount
Purified water balance <br/> Total 100

<Formulation example 3> Cream squash lily extract (bulb; amount of dry residue) 0.09
Beeswax 2.0
Stearyl alcohol 5.0
Stearic acid 8.0
Squalane 10.0
Self-emulsifying glyceryl monostearate 3.0
Polyoxyethylene cetyl ether (20E.0.) 1.0
Propylene glycol 5.0
Potassium hydroxide 0.3
Preservative / Antioxidant Appropriate amount Fragrance Appropriate amount
Purified water balance <br/> Total 100

<Formulation example 4> Funny liquid paraffin 2.0
Vaseline 1.0
Diisostearyl malate 1.0
Polyoxybutylene polyoxyethylene polyoxypropylene glyceryl ether (3B.O.) (8E.O.) (5P.O.) 0.2
Lauryl PEG-9 polydimethylsiloxyethyl dimethicone 0.5
PEG-9 Polydimethylsiloxyethyl dimethicone 0.2
Decamethylcyclopentasiloxane 4.0
Octyl methoxycinnamate 2.6
Diethylaminohydroxybenzoyl hexyl benzoate 1.0
Sericite 20.0
Zinc laurate 2.0
Boron nitride 8.0
Squash lily extract (bulb; dry residue) 0.01
Preservative appropriate amount
Talc balance <br/> Total 100

<Prescription Example 5> Packing agent lily extract (bulb; amount of dry residue) 0.002
Vinyl acetate resin emulsion 15.0
Polyvinyl alcohol 10.0
Jojoba oil 3.0
Glycerin 5.0
Titanium oxide 8.0
Kaolin 7.0
Ethanol 5.0
Perfume appropriate amount Preservative / antioxidant appropriate amount
Purified water balance <br/> Total 100

<Formulation Example 6> Ointment squash extract (bulb; dry residue amount) 0.001
Resorcin 0.5
Octyl paradimethylaminobenzoate 4.0
Butylmethoxybenzoylmethane 4.0
Stearyl alcohol 18.0
Owl 20.0
Glycerin monostearate 0.3
Vaseline 33.0
Perfume appropriate amount Preservative / antioxidant appropriate amount
Purified water balance <br/> Total 100

<Prescription Example 7> Hair Tonic Squash Extract (Bulb; dry residue) 0.01
Hardened castor oil EO (40) 2.0
Ethanol 70.0
Perfume appropriate amount
Purified water balance <br/> Total 100

<Prescription Example 8> Hair tonic polyethylene glycol 2000 2.0
L-Menthol 0.2
Squash lily extract (bulb; dry residue) 0.02
Ethanol 50.0
Perfume appropriate amount
Purified water balance
Total 100

<Prescription Example 9> Hair Liquid Polyoxypropyl Butyl Ether (40PO) 15.0
Polyoxypropyl butyl ether phosphate (40PO) 15.0
Squash lily extract (bulb; dry residue) 0.05
Ethanol 50.0
Perfume appropriate amount
Purified water balance <br/> Total 100

<Prescription Example 10> Hair Gel Carboxyvinyl Polymer 0.5
Polyvinylpyrrolidone 2.0
Glycerin 4.0
Squash lily extract (bulb; dry residue) 0.03
Polyoxyethylene octyldodecyl ether appropriate amount Sodium hydroxide 0.15
Ethanol 20.0
Perfume appropriate amount
Purified water balance
Total 100

<Formulation example 11> Scalp treatment 1,3-propylene glycol 0.5
Pentaerythritol tetra-2-ethylhexanate 1.2
Ethanol 60.0
Squash lily extract (bulb; dry residue) 0.02
Perfume appropriate amount
DME / LPG (95/5) appropriate amount
Total 100

<Prescription Example 12> Hairspray (stock solution)
Acrylic resin alkanolamine salt (50%) 7.0
Cetyl alcohol 0.1
Silicone oil 0.3
Ethanol 92.4
Squash lily extract (bulb; dry residue) 0.002
Perfume appropriate amount (filling)
Stock solution 50.0
Dimethyl ether 45.0
LPG 5.0
Total 100

Also in the above-mentioned prescription examples, a gray hair preventing effect and a gray hair improving effect were confirmed. In addition, although the above-mentioned prescription example showed the result of the extract at the time of extracting 50 (W / V)% ethanol aqueous solution about the above-ground part, a bulb, and a root, the same tendency was confirmed also in other parts and extraction solvents. .

Claims (3)

A tyrosinase activity promoter comprising an extract of a lily of the genus Lily belonging to the lily family. A melanin production promoter comprising an extract of a lily of the genus Lily belonging to the lily family. A white hair-preventing or white-hair-improving cosmetic comprising a lily of the genus Lily belonging to the lily family.