JP2016113460A - Oral antimicrobial agent and oral composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an oral antimicrobial agent and an oral composition exerting an excellent antibacterial effect that selectively inhibits the growth of pathogenic bacteria without affecting on the growth of a nonpathogenic indigenous bacteria in oral cavity.SOLUTION: The invention provides an oral antimicrobial agent comprising one or more kinds of plant extracts selected from Cistanche tubulosa, sprouting Brassica oleracea var. italica, and Coprinus comatus, and an oral composition containing the oral antimicrobial agent.SELECTED DRAWING: None

Description

  TECHNICAL FIELD The present invention relates to an antibacterial agent for oral cavity and a composition for oral cavity that exhibit an excellent selective antibacterial effect that selectively suppresses the growth of pathogenic bacteria without affecting the growth of non-pathogenic bacteria in the oral cavity.

  A variety of bacteria coexist in the human body, forming a bacterial population called the resident flora. Pathogenic bacteria and opportunistic bacteria are present in the resident bacterial flora, but in a state where the bacterial flora is maintained, diseases caused by these bacteria are suppressed and are in a so-called healthy state . However, when the balance of the bacterial flora is lost due to dietary habits, antibiotic intake, stress, aging, etc., pathogenic bacteria and opportunistic bacteria increase and develop diseases. Therefore, it is important to maintain the balance of the resident bacterial flora by selectively suppressing pathogenic bacteria and predominating non-pathogenic resident bacteria for disease control.

  Among the hundreds of bacteria that grow in the oral cavity of humans, the pathogenic bacteria are porphyromonas gingivalis, a periodontopathic bacterium, and Streptococcus mutans, a causative bacterium of caries. There are only a few types of bacteria represented by Streptococcus mutans). Most of the rest are Streptococcus gordonii, Streptococcus mitis, Streptococcus oralis, and M. streptococcus oralis, which are representative of the mitochondrion of M. ) Bacteria such as Veionella parvula, Neisseria subflava, and Neisseria bacteria such as Neisseria subflava are usually non-pathogenic indigenous bacteria. Therefore, in order to prevent oral diseases, it is important to selectively suppress the growth of oral pathogenic bacteria in the oral cavity without affecting the growth of non-pathogenic oral resident bacteria.

  In particular, the non-pathogenic resident Streptococcus of Mitsis group is known to be involved in the tooth surface adhesion of the above-mentioned caries and periodontal disease causative bacteria, or to have an antibacterial action ( Non-patent document 1) and utilization as probiotics are also disclosed (patent document 1; International Publication No. 2013/021957), which are resident bacteria that act on oral disease suppression.

  Therefore, techniques for selectively suppressing periodontopathic bacteria without affecting the growth of non-pathogenic oral resident bacteria are disclosed in Patent Documents 2 to 4 (WO2003 / 099304, JP2009- 274957 and JP2010-64961), but the effect is not sufficient.

International Publication No. 2013/021957 International Publication No. 2003/099304 JP 2009-274957 A JP 2010-64961 A International Publication No. 2008/093678 JP 2007-31297 A JP-T-2001-520181 JP 2012-72078 A JP 2012-56914 A

Van Hoogmoed CG et al., Applied Environmental Microbiology, 66 (2), p659-663, 2000

  Therefore, development of a new technique for selectively suppressing the growth of oral pathogenic bacteria without affecting the growth of non-pathogenic oral resident bacteria has been desired.

  The present invention has been made in view of the above circumstances, and an antibacterial agent for oral cavity that exhibits an excellent antibacterial effect that selectively suppresses the growth of pathogenic bacteria without affecting the growth of non-pathogenic bacteria in the oral cavity. It aims at providing the composition for oral cavity.

As a result of intensive studies to achieve the above object, the present inventors have found that a specific plant extract described later can selectively cause pathogenicity in the oral cavity without affecting the growth of non-pathogenic bacteria in the oral cavity. It has an excellent selective antibacterial action that suppresses the growth of bacteria, is effective as an antibacterial agent for oral cavity, and the selective antibacterial action causes an excessive increase in pathogenic bacteria that cause oral diseases. It can be controlled to prevent the balance of oral resident flora from being lost, and the oral resident flora can be controlled to maintain a healthy state, and this can be added to the oral composition. It has been found that the above-described excellent selective antibacterial effect can be imparted and can be suitably used for the prevention or suppression of oral diseases such as periodontal disease, and the present invention has been made.
In this case, according to the present invention, a non-pathogenic bacterium resident in the oral cavity, in particular, Streptococcus gordonii, Streptococcus mitis, Streptococcus olitis (Streptococcus oris) -linked sphere of M. istis Excellent antibacterial activity to selectively inhibit the growth of pathogenic bacteria in the oral cavity, especially periodontopathic bacteria such as Porphyromonas gingivalis and Prevotella intermedia, without affecting the growth of Has an effect. It can also be expected that the antibacterial action controls the normal bacterial flora in the oral cavity to maintain a healthy state.

  In addition, the extract of kankanikujuyo containing echinacoside and acteoside is effective as a hyaluronidase inhibitor (Patent Document 5; International Publication No. 2008/093678). It has oxidant and radical scavenging action and cell regeneration action and is effective as a compounding ingredient such as an external preparation for skin (Patent Documents 6 and 7; JP 2007-31297 A, JP 2001-520181 A), Extracts such as Sacrehito Toyotake containing ergothioneine have a melanin production inhibitory action and are effective as an antifungal agent by an anti-Candida action (Patent Documents 8 and 9; JP 2012-72078 A and JP 2012). -56914) is known, but is related to the present invention. The extract has an excellent antibacterial action that selectively suppresses the growth of oral pathogenic bacteria without affecting the growth of non-pathogenic oral resident bacteria, and is effective as an antibacterial agent for oral cavity This is a new finding of the present inventors.

Accordingly, the present invention provides the following oral antibacterial agent and oral composition.
[1]
An antibacterial agent for oral cavity comprising one or more plant extracts selected from Kankaniku Juyo, germinated broccoli, and Sasaclehi Toyotake.
[2]
The antibacterial agent for oral cavity according to [1], which is a selective antibacterial agent that suppresses the growth of pathogenic bacteria without suppressing the growth of non-pathogenic bacteria in the oral cavity.
[3]
The antibacterial agent for oral cavity according to [2], wherein the pathogenic bacteria are Porphyromonas gingivalis and / or Prevotella intermedia, which are periodontopathic bacteria, and the non-pathogenic bacteria are Streptococcus of the Mittis group.
[4]
The antibacterial agent for oral cavity according to [3], wherein the non-pathogenic bacterium is Streptococcus gordonii, Streptococcus mitis and Streptococcus oralis, a streptococcus of the Mittis group.
[5]
An oral composition comprising the oral antibacterial agent according to any one of [1] to [4].
[6]
When the antibacterial agent for oral cavity contains kankanikujuyo extract, the content is 0.001 to 10% by mass of the whole composition as a pure extraction amount, and when the germinated broccoli extract is contained, the content thereof Is a pure extract amount of 0.001 to 10% by mass of the whole composition, and when it contains Sasahihi toyoke extract, its content is 0.001 to 10% by mass of the whole composition in the extracted pure amount [ [5] The composition for oral cavity according to [5].
[7]
The oral composition according to [5] or [6], which is prepared in a dosage form selected from a dentifrice, mouthwash, oral spray, tablet, gummi, chewing gum and candy.

  According to the present invention, an antibacterial agent for oral cavity and a composition for oral cavity that exhibit excellent selective antibacterial effects that selectively suppress the growth of pathogenic bacteria without affecting the growth of non-pathogenic bacteria in the oral cavity. Can be provided.

The present invention will be described in further detail below. The antibacterial agent of the present invention uses, as an active ingredient, an extract of one or more kinds of plants selected from (a) Kankaniku Juyo, (b) germinated broccoli and (c) Sasakuhi Toyota. This is a selective antibacterial agent, and has an excellent antibacterial effect that selectively suppresses the growth of periodontopathic bacteria without affecting the growth of non-pathogenic bacteria in the oral cavity. Also acts as a control agent.
Here, “selective antibacterial” refers to the growth of non-pathogenic bacteria by having antibacterial activity against pathogenic bacteria in the oral cavity while having no antibacterial activity against non-pathogenic bacteria. It is an antibacterial action that selectively inhibits the growth of pathogenic bacteria without inhibition.
Moreover, “oral resident flora control” means that the growth of non-pathogenic resident bacteria is not affected by exerting selective antibacterial action against pathogenic bacteria as described above. It is to control the microbial flora balance of the resident flora to a low pathogenic flora.

  (A) Cistanche tubulosa is a genus Cypridaceae, and is a plant parasitic on the roots of red willow (Village: Goryuaceae) with its origin in China and the Taklamakan Desert.

As for the extract of Kankaniku Juyo, those extracted by known methods can be used, but commercially available products can also be used. A preferable extract of citrus cucumber is extracted using a hydrophilic solvent, for example, a hydrophilic organic solvent such as water, a lower aliphatic alcohol having 1 to 3 carbon atoms such as ethanol and methanol, or a mixture thereof. The ones made are preferred. The extraction method is not particularly limited, and any method such as continuous extraction, immersion extraction or supercritical extraction can be employed. The extraction temperature may be from room temperature to reflux heating, and a normal extraction device can be used. .
For example, it can be prepared by employing the method described in International Publication No. 2008/093678 of Patent Document 5. Specific examples are shown below.
The stems of Cistanche tubulosa, more preferably the fresh stems of the plant, are cut into flakes or ground into fine particles or powder. Next, the obtained flakes, fine particles, or powder is immersed in a hydrophilic solvent (water, an organic solvent such as a lower aliphatic alcohol having 1 to 3 carbon atoms such as ethanol, methanol, or a mixture thereof). Of these hydrophilic solvents, water-containing ethanol is particularly preferable. The hydrous ethanol has an ethanol concentration of 20 to 60% by mass, desirably about 40 to 50% by mass. The mixed solution is filtered, and the filtrate is concentrated under reduced pressure or in vacuo to obtain a crude extract. Further, the crude extract is heated in water and then transferred to an adsorption column packed with D-101 type or AB-8 type macroporous adsorption resin to purify the extract. The eluate is concentrated and then dried by a known drying method to obtain the above extract.

  In addition, the kankanikujuyou extract can also use commercial items, such as the product made by Oriza Oil Chemical Co., Ltd.

  (B) Germination broccoli is a germination body of the broccoli (Brassica oleracea var. Italica) which belongs to the Brassicaceae Brassica genus, a production area and a cultivation method are not specifically limited, A various thing can be used.

The extract of germinated broccoli can be extracted by a known method, but a commercially available product can also be used. A preferred germinated broccoli extract was extracted using a hydrophilic solvent, for example, water, a hydrophilic organic solvent such as ethanol or methanol having 1 to 3 carbon atoms, or a mixture thereof. Those are preferred. The extraction method is not particularly limited, and any method such as continuous extraction, immersion extraction or supercritical extraction can be employed. The extraction temperature may be from room temperature to reflux heating, and a normal extraction device can be used. .
The method for preparing the germinated broccoli extract is not particularly limited, and a part or whole of the broccoli germination (buds, cotyledons, seeds, roots) or the dried and pulverized one is extracted with a solvent or the like. Can be obtained.
For example, it can be prepared by adopting the method described in Japanese Patent Application Laid-Open No. 2007-31297 of Patent Document 6. Specific examples are shown below.
Broccoli (Brassica oleracea var. Italica) seeds were immersed in water at 15 to 25 ° C., germinated by indirect sunlight irradiation for 2 to 5 days, and then whole plants (buds, Cotyledons, seeds, roots). This is dried and pulverized and then degreased. Thereafter, the powder obtained is finely pulverized, sterilized and sieved, and the obtained powder is treated with a hydrophilic solvent (solvent, for example, an organic solvent such as water, lower aliphatic alcohol having 1 to 3 carbon atoms such as ethanol, or a mixture thereof). Soak to obtain a filtrate. Of these hydrophilic solvents, water-containing ethanol or water-containing 1,3-butylene glycol is particularly preferable. The filtrate can be concentrated under reduced pressure or in vacuum to obtain a germinated broccoli extract. As a preferred extraction method, water-containing ethyl alcohol or water-containing 1,3-butylene glycol is used, extracted at room temperature or heated for 1 to 10 days, filtered, and the obtained filtrate is left for about one week. And then aging and filtering again. If necessary, this extract may be further pulverized by adding a step such as decolorization and deodorization to distill off the solvent.

  In addition, the extract of germination broccoli can also use a commercial item, such as the product made by Oriza Yuka.

  (C) Coprinus comatus is an edible moth belonging to the genus Sacrahitococeae and has a cylindrical white silk umbrella and is covered with white fur-like scales. Widely distributed in temperate regions around the world such as Europe and North America, it also grows naturally in Japan from spring to autumn.

As for the extract of Sasakuhi Toyotake, one extracted by a known method as shown below can be used, but a commercially available product can also be used. A preferable extract of Sasahihi Toyotake was extracted using a hydrophilic solvent, for example, water, a hydrophilic organic solvent such as ethanol or methanol having a carbon number of 1 to 3 or the like, or a mixture thereof. Those are preferred. The extraction method is not particularly limited, and any method such as continuous extraction, immersion extraction or supercritical extraction can be employed. The extraction temperature may be from room temperature to reflux heating, and a normal extraction device can be used. .
The extract of Sasa Kurehi Toyotake uses a hydrophilic solvent (water, an organic solvent such as ethanol or methanol having a carbon number of 1 to 3 such as methanol, or a mixture thereof), continuous extraction, immersion extraction, Any method such as supercritical extraction can be employed, and any device can be used at room temperature to reflux heating.
For example, it can be prepared by adopting the method described in JP 2012-72078 A of Patent Document 8. Specific examples are shown below.
A Sacrihitoke (Coprinus comatus) is immersed in a hydrophilic solvent (an organic solvent such as water, ethanol, methanol or a lower aliphatic alcohol having 1 to 3 carbon atoms, or a mixture thereof) to obtain a filtrate. Of these hydrophilic solvents, water or water-containing ethanol is particularly preferable. In particular, water is preferable because it is difficult to reduce the activity of the active ingredient during extraction. The filtrate can be concentrated under reduced pressure or in vacuum to obtain an extract of Sasahi Toyota. If necessary, this extract may be further pulverized by adding a step such as decolorization and deodorization to distill off the solvent.

  In addition, the extract of Sasa Kurehi Toyotake can also use commercially available products, such as those manufactured by Oriza Oil Chemical Co., Ltd.

  The plant extract selected from the above (a) to (c) according to the present invention can be suitably blended in the oral composition as the selective antibacterial agent of the present invention.

  In the present invention, the composition for oral cavity includes tablets, gummies, and the like applied to the oral cavity in addition to general dentifrice, mouthwash, mouse spray, oral paste, ointment, patch and the like. It includes candy, chewing gum and the like, and can be prepared and used in various forms such as paste, gel, liquid and solid.

  Specific examples of oral compositions include toothpastes, toothpastes, liquid toothpastes, liquid toothpastes, moisturized toothpastes, mouthwashes, mouse sprays, oral pastes, ointments, patches, tablets, troches, It can be prepared in various dosage forms such as tablets such as tablets, gummi, candy, granules, chewing gum and the like. In particular, it is suitably prepared as a dentifrice, mouthwash, tablet, gummi, and candy, particularly as a dentifrice and mouthwash. In addition, it can also be suitably used as a tablet, gummi, or candy in terms of imparting an antibacterial effect.

In the above-mentioned composition for oral cavity, the blending amount of the extract of Kankaniku Juyo is preferably 0.001 to 10% (mass%, the same applies hereinafter), more preferably 0.01 to 5%, and further Preferably it is 0.1 to 1%.
The blending amount of the extract of germinated broccoli is preferably 0.001 to 10%, more preferably 0.01 to 5%, and still more preferably 0.1 to 1%.
0.001 to 10% is preferable, and the blending amount of the extract of Sasakuhi Toyotake is preferably 0.01 to 5%, more preferably 0.1 to 1%.
In addition, the compounding quantity of the extract in this invention is the extraction pure part quantity remove | excluding the extraction solvent from the extract (hereinafter the same).

  In this invention, when mix | blending the plant extract chosen from (a), (b), (c), the more excellent antimicrobial effect can be provided as the compounding quantity is in the said range. In addition, the antibacterial effect improves as the blending amount of the plant extract increases, but if the blending amount is too large, the taste of itself may be manifested. Is more preferred.

In addition to the above-mentioned components, the oral composition may be blended with other known components as appropriate according to the dosage form as necessary within the range not impeding the effects of the present invention, and prepared by a usual method. .
In the dentifrice, abrasives, thickeners, binders, surfactants and, if necessary, sweeteners, colorants, preservatives, fragrances, active ingredients, and the like can be blended.
A mouthwash can contain a thickener, a binder, a surfactant, and, if necessary, a sweetener, a colorant, a preservative, a fragrance, an active ingredient, and the like.
Tablets include sugar alcohols such as sorbitol and maltitol, excipients such as cellulose, lactose and dextrin, fine silicon dioxide, acidulant, sweetener, colorant, emulsifier, thickener, gelling agent, fruit juice, spice Active ingredients can be blended.
Gummy can contain thickeners such as glycerin and gelatin, gelling agents, sugars, acidulants, sweeteners, colorants, emulsifiers, fruit juices, active ingredients, and the like.
The chewing gum can contain a binder such as a gum base and an edible gum, a sweetener, a coloring agent, a sour agent, a preservative, a brightener, a fragrance, an active ingredient, and the like, and may be coated with sugar coating as necessary.

  Examples of the abrasive used in the dentifrice include silica-based abrasives, calcium phosphate-based abrasives, calcium carbonate-based abrasives, and the blending amounts are usually 2 to 50% for toothpaste and 0 to 0 for liquid toothpaste. 30%.

  Examples of the thickener include sugar alcohols such as sorbitol and xylitol, and polyhydric alcohols such as glycerin and propylene glycol. The blending amount is usually 5 to 50%.

  As the binder, organic or inorganic binders such as cellulose derivatives such as sodium carboxymethyl cellulose, gums such as xanthan gum, gelling silica, and gelling aluminum silica can be blended. The blending amount is usually 0.5 to 10%.

  As the surfactant, an anionic surfactant, a nonionic surfactant, a cationic surfactant, and an amphoteric surfactant that are generally used in oral compositions can be blended. Examples of the anionic surfactant include alkyl sulfates such as sodium lauryl sulfate and N-acyl sarcosinate. Nonionic surfactants include sugar fatty acid esters, sugar alcohol fatty acid esters, sorbitan fatty acid esters, glycerin fatty acid esters, polyoxyethylene fatty acid esters such as polyoxyethylene hydrogenated castor oil, polyoxyethylene higher alcohol ethers, fatty acid alkanolamides, etc. Is mentioned. Examples of cationic surfactants include alkylammonium salts, and examples of amphoteric surfactants include betaine-based and imidazoline-based ones. The compounding amount of the surfactant is usually 0 to 10%, particularly 0.01 to 5%.

Examples of the sweetening agent include saccharin sodium.
Examples of the colorant include Red No. 2, Blue No. 1, and Yellow No. 4, and examples of the preservative include paraoxybenzoic acid ester.

Perfumes include peppermint oil, spearmint oil, anise oil, eucalyptus oil, winter green oil, cassia oil, clove oil, thyme oil, sage oil, lemon oil, orange oil, peppermint oil, cardamom oil, coriander oil, mandarin oil, Lime oil, lavender oil, rosemary oil, laurel oil, camomil oil, caraway oil, marjoram oil, bay oil, lemongrass oil, origanum oil, pine needle oil, neroli oil, rose oil, jasmine oil, grapefruit oil, sweetie Natural fragrances such as oil, coconut oil, Iris concrete, absolute peppermint, absolute rose, orange flower, and processing of these natural fragrances (front reservoir cut, rear reservoir cut, fractional distillation, liquid-liquid extraction, essence, powder Perfumes, etc.) and menthol, Rubon, Anethole, Cineol, Methyl salicylate, Synamic aldehyde, Eugenol, 3-1-Mentoxypropane-1,2-diol, Thymol, Linalol, Linarel acetate, Limonene, Menthone, Menthyl acetate, N-Substituted paramenthane 3-carboxamide, pinene, octyl aldehyde, citral, pulegone, carbyl acetate, anisaldehyde, ethyl acetate, ethyl butyrate, allylcyclohexane propionate, methyl anthranilate, ethyl methyl phenyl glycidate, vanillin, undecalactone, Hexanal, butanol, isoamyl alcohol, hexenol, dimethyl sulfide, cycloten, furfural, trimethylpyrazine, ethyl lactate, ethyl Single flavors such as oacetate, and other flavors such as strawberry flavor, apple flavor, banana flavor, pineapple flavor, grape flavor, mango flavor, butter flavor, milk flavor, fruit mix flavor, tropical fruit flavor, etc. Known perfume materials used in the composition for use can be used.
The amount of these fragrances is usually 0.00001 to 1% for dentifrices and mouthwashes, and 0.001 to 50% for tablets, gummi and chewing gum, and the above fragrance materials were used. It is preferable to use 0.1 to 10% of the flavoring fragrance during the composition.

  As an active ingredient, the well-known thing normally mix | blended with the composition for oral cavity can be mix | blended in the range which does not inhibit the effect of this invention. Active ingredients include nonionic fungicides such as isopropylmethylphenol, cationic fungicides such as cetylpyridinium chloride, anti-inflammatory agents such as tranexamic acid and epsilon aminocaproic acid, enzymes such as dextranase, sodium fluoride, mono Examples include fluorides such as sodium fluorophosphate, water-soluble phosphate compounds, copper compounds, potassium nitrate, aluminum lactate, various vitamins, plant extracts, and the like. In addition, the compounding quantity of the said active ingredient is set in the range which does not prevent the selective antimicrobial effect of this invention.

  In addition, in the composition for oral cavity of this invention, it is preferable to restrict | limit the mixing | blending of the non-selective disinfectant which disinfects non-pathogenic bacteria with periodontopathic bacteria. The non-selective bactericides are often found in bactericides that have been widely used in oral preparations, and examples thereof include cationic bactericides such as cetylpyridinium chloride, benzethonium chloride, and benzalkonium chloride. Moreover, nonionic disinfectants, such as isopropylmethylphenol, are also mentioned. These non-selective bactericides can be blended within a range where the oral composition of the present invention has a selective antibacterial effect, but when blended, 0.1% or less, especially 0.05% of the total composition. The following are preferred, most preferably not blended. These non-selective bactericides have a non-selective bactericidal action that kills pathogenic periodontopathic bacteria as well as non-pathogenic oral resident bacteria. If this occurs, the bacterial balance in the oral cavity may be lost, and the oral microbial flora cannot be controlled, resulting in the possibility of bacterial substitution. Therefore, in the present invention, it is more suitable for balance control of the oral microbial flora by the selective antibacterial effect that the non-selective fungicide is not blended.

  Hereinafter, although an experiment example and a formulation example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to the following Example. In the following examples, “%” means “% by mass” unless otherwise specified.

[Experimental example]
By the method shown below, the antibacterial (growth suppression) effect of the plant extract of each density | concentration (pure extraction part) shown in Table 1 was evaluated. The results are shown in the table.
100 μL each of plant extracts prepared with distilled water to 0.002%, 0.02%, 0.2% concentration (pure extract) was added to a 96-well plate. Next, 100 μL of the bacterial solution shown in the table was added to each, followed by anaerobic culture (80 vol% nitrogen, 10 vol% carbon dioxide, 10 vol% hydrogen) at 37 ° C. for 24 hours. The bacterial solution was pre-cultured with Todd Hewitt Broth (Becton and Dickinson) culture solution (THBHM medium) containing 5 mg / L hemin (manufactured by Sigma) and 1 mg / L vitamin K (manufactured by Wako Pure Chemical Industries). The inoculated bacteria were inoculated at a concentration of 2% in a 2-fold concentration THBHM medium.
Further, the amount of bacterial growth was measured by measuring the turbidity at a wavelength of 550 nm, and calculating the relative value of the control (plant extract unadded: 0%) relative to the turbidity as the bacterial growth. That is, the growth is promoted as the growth degree is increased, and the growth is inhibited as the growth degree is decreased.
In the present evaluation method, the selective antibacterial effect in the present invention is the following bacteria used for the evaluation,
The growth of all non-pathogenic bacteria is 0.8 or more, preferably 1.0 or more, and the growth of all pathogenic bacteria is 0.8 or less, preferably 0.5 or less, more preferably 0 .1 or less.
Furthermore, the antibacterial effect was evaluated by the same method as described above using each concentration of cetylpyridinium chloride shown in Table 2, and the results are also shown in the table.

Oral resident bacteria used for the evaluation are the following 5 bacterial species purchased from American Type Culture Collection.
Non-pathogenic bacteria:
Streptococcus gordonii ATCC 10558 (hereinafter abbreviated as S. gordonii)
Streptococcus mitis ATCC 47456 (hereinafter abbreviated as S.mitis)
Streptococcus oralis ATCC 9811 (hereinafter abbreviated as S. oralis)
Pathogenic bacteria:
Porphyromonas gingivalis ATCC 33277 (hereinafter abbreviated as P. gingivalis)
Prevoterra intermedia ATCC 49046 (hereinafter abbreviated as P.intermedia)
Before measuring the turbidity, the plate was shaken to stir the culture solution.

The details of the extract used and the components contained in the extract are as follows.
-As an extract of Cistanche tubulosa, Kanka extract manufactured by Oriza Oil Chemical Co., Ltd. (Kanka extract-P25, pure extract amount of Kanka extract 100%) was used.
-As an extract of germinated broccoli (Brassica oleracea var. Italica), broccoli sprout extract (broccoli sprout extract, 50% pure extract of germinated broccoli extract) manufactured by Oriza Oil Chemical Co., Ltd. was used.
As extracts of Coprinus comatus, Coprino Extract (registered trademark) (Coprino Extract (registered trademark) -P0.5, manufactured by Oriza Oil Chemical Co., Ltd.) %)It was used.
In the reference example, cetylpyridinium chloride manufactured by Wako Pure Chemical Industries, Ltd. was used.

  From the results shown in Tables 1 and 2, periodontal pathogens are selectively used without affecting the growth of Streptococcus of the Mittis group in the oral cavity by the extract of Kankaniku Juyo, germinating broccoli, or Saccharum terrestris. It has been found that a selective antibacterial effect that suppresses the growth of sexual bacteria is exhibited. In addition, the antibacterial effect prevents the pathogenic bacteria from increasing excessively, prevents the oral resident flora from being unbalanced, and controls the oral resident flora to maintain a healthy state Was expected.

  Next, the composition for oral cavity of the composition shown to the following prescription example was prepared using the plant extract similar to the above. All oral compositions exhibited an excellent antibacterial effect.

[Prescription Example 1] Dentifrice Silicic anhydride 20.0%
Sodium lauryl sulfate 1.0
Propylene glycol 3.0
Sorbit (100% product) 30.0
Xanthan gum 1.0
Saccharin sodium 0.1
Fragrance 1.0
Germinated broccoli extract 0.01
Water balance
Total 100.0%

[Prescription Example 2] mouthwash xylitol 3.0%
Glycerin (100% product) 5.0
Polyoxyethylene (60) hydrogenated castor oil 0.5
Sodium citrate 0.3
Citric acid 0.1
Fragrance 0.2
Ethanol 8.0
Propylene glycol 3.0
Kankaniku Juyo Extract 0.01
Water balance
Total 100.0%

[Prescription Example 3] Tablets (tablets)
76.0% sugar
Glucose 20.0
Sucrose fatty acid ester 0.2
Kankaniku Juyo Extract 0.1
Water balance
Total 100.0%

[Prescription Example 4] Tablet Lactose 55.0%
Crystalline cellulose 25.0
Starch degradation product 10.0
Glycerin fatty acid ester 5.0
Sasakurehi Toyotake Extract 0.05
Water balance
Total 100.0%

[Prescription Example 5] Gummy reduced starch syrup 40.0%
Granulated sugar 20.0
Glucose 20.0
Gelatin 5.0
Dye 0.02
Fragrance 0.5
Germinated broccoli extract 0.1
Water balance
Total 100.0%

[Formulation Example 6] Candy Sugar 50.0%
Minamata 33.0%
Organic acid 2.0%
Fragrance 0.5%
Sasakurehi Toyotake Extract 1.0%
Water balance
Total 100.0%

[Formulation example 7] Chewing gum sugar 50.0%
Gum base 20.0
Glucose 14.0
Minamata 15.0
Fragrance 0.5
Kankaniku Juyo Extract 0.5
Water balance
Total 100.0%

[Formulation Example 8] Oral pasta hydroxyethyl cellulose 3.0%
Carrageenan 1.0
Sorbit (70% aqueous solution) 40.0
Sucrose palmitic acid monoester 2.0
Lauroyl sarcosine sodium 0.3
Aluminum lactate 0.8
Saccharin sodium 0.1
Sodium tartrate 1.0
Kankaniku Juyo Extract 1.0
Fragrance 1.0
Hydrochloric acid or sodium hydroxide
Water balance
Total 100.0%

Claims (7)

  An antibacterial agent for oral cavity comprising one or more plant extracts selected from Kankaniku Juyo, germinated broccoli, and Sasaclehi Toyotake.   The antibacterial agent for oral cavity according to claim 1, which is a selective antibacterial agent that suppresses the growth of pathogenic bacteria without suppressing the growth of non-pathogenic bacteria in the oral cavity.   The antibacterial agent for oral cavity according to claim 2, wherein the pathogenic bacterium is periodontopathic bacterium Porphyromonas gingivalis and / or Prevotella intermedia, and the non-pathogenic bacterium is Streptococcus of Mittis group.   4. The antibacterial agent for oral cavity according to claim 3, wherein the non-pathogenic bacterium is Streptococcus gordonii, Streptococcus mitis and Streptococcus oralis, Streptococcus.   The composition for oral cavity containing the antimicrobial agent for oral cavity of any one of Claims 1-4.   When the antibacterial agent for oral cavity contains kankanikujuyo extract, the content is 0.001 to 10% by mass of the whole composition as a pure extraction amount, and when the germinated broccoli extract is contained, the content thereof Is 0.001 to 10% by mass of the whole composition in terms of the extracted pure amount, and when it contains Sasahihito Toyotake extract, the content is 0.001 to 10% by mass of the whole composition in terms of the extracted pure amount. Item 6. The oral composition according to Item 5.   The composition for oral cavity according to claim 5 or 6, prepared in a dosage form selected from dentifrice, mouthwash, spray for oral cavity, tablet, gummi, chewing gum and candy.