JP2016106621A - Producing method of polyphenol compound containing plant, method of increasing amount of polyphenol compound in polyphenol compound containing plant, method of increasing amount of tricaffeoylquinic acid in tricaffeoylquinic acid containing plant and tricaffeoylquinic acid containing plant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a producing method of polyphenol compound containing plant in which plant containing increased polyphenol compound amount than normal amount may be efficiently produced, a method of increasing amount of polyphenol compound in polyphenol compound containing plant, a method of increasing amount of tricaffeoylquinic acid in tricaffeoylquinic acid containing plant and a tricaffeoylquinic acid containing plant.SOLUTION: There is provided a producing method and application thereof of polyphenol compound containing plant containing increased polyphenol compound amount than a plant subjected to root part removing step, comprising a root part removing step in which root part of grown polyphenol compound containing plant selected from a group consisting of potatoes other than sweet potato, convolvulaceae plant and asteraceae plant and a preserving step in which the plant from which root part is removed is preserved under the presence of water, at 20°C to 40°C, for 24 hours or more.SELECTED DRAWING: None

Description

  The present invention is a method for producing a polyphenol compound-containing plant, a method for increasing the amount of a polyphenol compound in a polyphenol compound-containing plant, a method for increasing the amount of tricaffeoylquinic acid in a tricaffeoylquinic acid-containing plant, and The present invention relates to a tricaffeoylquinic acid-containing plant.

  The polyphenol compound is contained in leaves, stems, flowers, berries, seeds, etc. of plants such as tea tree, coffee tree, sweet potato, spring chrysanthemum, burdock, soybean, and grape. A polyphenol compound is a general term for substances having a plurality of phenolic hydroxyl groups in the molecule. Since polyphenol compounds have an antioxidant action, they are used in many health foods, cosmetics and the like.

Attempts to increase the content of polyphenol compounds in plants have been made in the past, and various reports have been made.
For example, Patent Document 1 describes that the content of anthocyanin, which is a kind of polyphenol compound, can be increased by irradiating a sunny lettuce during cultivation with blue light having a wavelength of 400 nm to 500 nm at night. There is.
In Patent Literature 2, hydroponic cultivation in which hydroponic cultivation under artificial lighting is grown is replaced with water just before harvesting, and hydroponic cultivation in which light is irradiated for 17 hours or more per day for one day or more. There is a description that the content of polyphenol compounds in vegetables can be increased by continuing.
Patent Document 3 describes that a crop having a high polyphenol content can be obtained by cutting a plant from which the above-ground growth points and roots of the plant have been completely removed.

  In recent years, it has been reported that caffeoylquinic acid compound, a kind of polyphenol compound, has various physiological activities such as prevention of dementia, anti-obesity, anti-hyperglycemia, anti-hypertension, anti-tumor, antibacterial, etc. Has attracted attention. Tricaffeoylquinic acid in particular has high physiological activity among caffeoylquinic acid compounds, and its effectiveness against various diseases has been demonstrated by tests using small animals (for example, see Non-Patent Document 1). Therefore, it can be said that its utility value is high.

  Therefore, if the content of the polyphenol compound in the plant can be increased, the physiological activity of the polyphenol compound can be further effectively utilized.

JP 2003-204718 A International Publication No. 2010/140632 JP 2007-14295 A

Makoto Yoshimoto, “Functional components of sweet potato, especially polyphenols”, Food and Technology, August 2008, pp10-18

  However, in the method described in Patent Document 1, the content of anthocyanins in the sunny lettuce can be increased, but the increase is small. In the method described in Patent Document 2, it is difficult to increase the productivity of vegetables because the vegetable cultivation method is limited to hydroponics. In the method described in Patent Document 3, it is necessary to completely remove the plant body above-ground growth point, and therefore the operation becomes very complicated. That is, none of the methods described in Patent Documents 1 to 3 is a sufficient method from the viewpoint of efficiently increasing the polyphenol compound in the plant body.

This invention is made | formed in view of the above situations, and provides the production method of the polyphenol compound containing plant body which can produce efficiently the plant body which increased the amount of polyphenol compounds rather than the quantity originally contained. The task is to do.
Another object of the present invention is to provide a method for increasing the amount of a polyphenol compound in a polyphenol compound-containing plant and a method for increasing the amount of tricaffeoylquinic acid in a tricaffeoylquinic acid-containing plant. .
Furthermore, an object of the present invention is to provide a tricaffeoylquinic acid-containing plant in which the amount of tricaffeoylquinic acid is increased from the amount originally contained.

The present invention is as follows.
[1] A root removing step for removing a root part of a grown polyphenol compound-containing plant selected from the group consisting of potatoes other than sweet potatoes, convolvulaceae and asteraceae, and a plant from which the root is removed, The preservation | save process preserve | saved at 20 degreeC-40 degreeC for 24 hours or more in presence of this, The production method of the polyphenol compound containing plant body in which the amount of polyphenol compounds increased rather than the plant body used for the said root removal process.
[2] The polyphenol compound-containing plant body from which the root portion is removed in the root removal step is a caffeoylquinic acid compound-containing plant body, and the caffeoylquinic acid compound-containing plant body used in the root removal step is caffeoylquinic acid. The production method according to [1], wherein the amount of the compound is increased.
[3] The polyphenol compound-containing plant from which the root is removed in the root removal step is a tricaffeoylquinic acid-containing plant, and the tricaffeoylquinic acid-containing plant used in the root removal step is tricaffeoylquina. The production method according to [1], wherein the acid amount is increased.

[4] The production method according to any one of [1] to [3], wherein the storage period in the storage step is 2 days to 35 days.
[5] The production method according to any one of [1] to [4], wherein the storage period in the storage step is 3 to 7 days.
[6] The production method according to any one of [1] to [5], wherein the storage step includes irradiating light to the plant body from which the root portion has been removed.
[7] The production method according to [6], wherein the illuminance of light in the light irradiation is 1,000 lux or more.
[8] The production method according to any one of [1] to [7], wherein the storage temperature in the storage step is 20 ° C to 35 ° C.

[9] The storage step according to any one of [1] to [8], wherein the storage step includes storing at least a part of the plant from which the root portion has been removed in at least one of water and soil. Production method.
[10] Any one of [1] to [9], wherein the storage step includes storing at least a part of the plant body from which the root portion has been removed by placing the plant in water and then placing the plant in soil. The production method according to one.
[11] The production method according to any one of [1] to [10], wherein the plant body is a convolvulaceae plant.
[12] The production method according to [11], wherein the convolvulaceae plant is sweet potato.

[13] Produced by the production method according to any one of [3] to [12], the content of tricaffeoylquinic acid is per 100 g of dry weight of the leaf blade of the tricaffeoylquinic acid-containing plant. Tricaffeoylquinic acid-containing plant that is 80 mg or more.
[14] A root removal step for removing a root of a grown polyphenol compound-containing plant selected from the group consisting of potatoes other than sweet potatoes, convolvulaceae and asteraceae, and a plant from which the root has been removed, The preservation | save process preserve | saved at 20 to 40 degreeC for 24 hours or more in presence of this, The method of increasing the amount of the polyphenol compound in a polyphenol compound containing plant body.
[15] A root removing step for removing the root of a plant containing tricaffeoylquinic acid grown from the group consisting of potatoes other than sweet potato, convolvulaceae, and asteraceae, and a plant from which the root has been removed A method for increasing the amount of tricaffeoylquinic acid in a tricaffeoylquinic acid-containing plant, comprising a storage step of storing the licoric acid at 20 ° C to 40 ° C for 24 hours or more in the presence of water.

ADVANTAGE OF THE INVENTION According to this invention, the production method of the polyphenol compound containing plant body which can produce efficiently the plant body in which the amount of polyphenol compounds increased rather than the quantity originally contained can be provided.
In addition, according to the present invention, it is possible to provide a method for increasing the amount of polyphenol compound in a polyphenol compound-containing plant and a method for increasing the amount of tricaffeoylquinic acid in a tricaffeoylquinic acid-containing plant. .
Furthermore, according to the present invention, it is possible to provide a tricaffeoylquinic acid-containing plant in which the amount of tricaffeoylquinic acid is increased from the amount originally contained.

  Hereinafter, specific embodiments of the present invention will be described in detail. However, the present invention is not limited to the following embodiments, and may be implemented with appropriate modifications within the scope of the object of the present invention. be able to.

In the present specification, a numerical range indicated using “to” means a range including the numerical values described before and after “to” as a minimum value and a maximum value, respectively.
In this specification, the amount of each component in the composition is the total amount of the plurality of substances present in the composition unless there is a specific indication when there are a plurality of substances corresponding to each component in the composition. Means.
In this specification, the term “process” is not limited to an independent process, and is included in the term if the intended purpose of the process is achieved even when it cannot be clearly distinguished from other processes. .

In the present specification, the “root” means a part of a plant that absorbs moisture and nutrients. When the plant body is cultivated in soil culture, at least a part thereof is in the soil, and in the case of hydroponics, at least a part thereof is in water (or hydroponics).
In the present specification, the “terrestrial part” means a part of a plant other than the above “root part”. The “aboveground part” includes a stem or stem, leaves that perform photosynthesis and water transpiration, and the like.

[Production Method of Polyphenol Compound-Containing Plant]
The method for producing a polyphenol compound-containing plant of the present invention (hereinafter also referred to as “plant production method”) is a grown polyphenol selected from the group consisting of potatoes other than sweet potatoes, convolvulaceae plants, and asteraceae plants. A root removing step for removing the root of a compound-containing plant (hereinafter, also simply referred to as “plant”) and the plant from which the root has been removed, in the presence of water at 20 ° C. to 40 ° C. for 24 hours or more. A method for producing a polyphenol compound-containing plant having an increased amount of polyphenol compound as compared to the plant subjected to the root removal step.
The plant body production method of the present invention may include other steps other than the root removal step and the storage step, if necessary, as long as the effects of the present invention are not impaired.

In the method for producing a plant of the present invention, after removing the root of the grown polyphenol compound-containing plant, the plant from which the root has been removed is stored at 20 ° C. to 40 ° C. for 24 hours or more in the presence of water. By such a simple method, it is possible to efficiently produce a plant body in which the amount of the polyphenol compound is increased from the amount originally contained.
In the plant production method of the present invention, the amount of the polyphenol compound is preferably 1.1 times or more, more preferably 1.3 times or more, and further preferably 1.4 times or more than the plant body subjected to the root removal step. Plant can be produced.
In the method for producing a plant of the present invention, the amount of caffeoylquinic acid compound is preferably 1.2 times or more, more preferably 1.5 times or more, and further preferably 2 than that of the plant subjected to the root removal step. Plants increased by more than 0 times can be produced.
Furthermore, in the method for producing a plant of the present invention, the amount of tricaffeoylquinic acid is preferably 1.2 times or more, more preferably 2.0 times or more, more preferably 3 than that of the plant subjected to the root removal step. Plants increased by more than 0 times can be produced.

The polyphenol compound-containing plant that can be applied to the method for producing a plant of the present invention includes a plant selected from the group consisting of potatoes other than sweet potatoes, convolvulaceae and asteraceae.
Examples of potatoes (excluding sweet potato) include potato, cassava, taro, taro, yam and the like.
Examples of the Convolvulaceae plant include sweet potato, convolvulus, morning glory, yorgao, hydrangea, liquorice, prunus, evolvulus and ensai.
Examples of Asteraceae plants include: Jerusalem artichoke, yarrow, burdock, mugwort, aster, baccaris, daisies, calendula, ezogiku, safflower, cornflower, nemophila, shungiku, margaret, sea anemone, chicory, mulchicore, datura , Dahlia, purple barrengiku, himejoon, fujibakama, tsubebuki, gerbera, hakogusa, miyakowasle, sunflower, yomena, lettuce, senbonyari, chamomile, cineraria, fuchsia, yacon, gypsophila, nogeshi, ursinia, hakuti
Each of the above-mentioned plant bodies contains a caffeoylquinic acid compound (including tricaffeoylquinic acid) and can be applied to the plant production method of the present invention.

Among the above, the polyphenol compound-containing plant that can be applied to the plant production method of the present invention is preferably a plant selected from the group consisting of a convolvulaceae plant and an Asteraceae plant, and is a convolvulaceae plant. Is more preferable, and it is still more preferable that it is a sweet potato.
Examples of sweet potatoes that can be preferably applied to the plant production method of the present invention include venezumas, beniharka, benicomachi, red and red, naruto kinki, white yutaka, white sasatsuma, koganesengan, murasakimasari, ayamurasaki, suou, shimoimo, tamakane There can be mentioned many varieties derived from the high system No. 14. In addition, sweet potatoes of various strains that have not yet been registered as varieties can also be applied to the plant production method of the present invention.

The polyphenol compound in the plant body that can be increased by the production method of the plant body of the present invention includes caffeoylquinic acid compound, caffeoylglucose compound, feruloylquinic acid compound, cinalin, rosmarinic acid, catechin compound, isoflavone compound, Examples include quercetin, luteolin, anthocyanin compounds, proanthocyanidin compounds, rutin compounds, flavangenol compounds, resveratrol compounds, tannic acid compounds, ellagic acid, curcumin, lignan compounds, and glycosides thereof.
In the method for producing a plant body of the present invention, as a polyphenol compound that can be increased in comparison with a normal method for producing a plant body, a caffeoylquinic acid compound can be mentioned.
Examples of caffeoylquinic acid compounds include monocaffeoylquinic acid (3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, and 1-O-caffeoyl oil. Quinic acid), dicaffeoylquinic acid (3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, 1,3- O-dicaffeoylquinic acid, 1,4-O-dicaffeoylquinic acid, and 1,5-O-dicaffeoylquinic acid), tricaffeoylquinic acid (3,4,5-O-tricaffee) Oil quinic acid, 1,4,5-O-tricaffeoylquinic acid, 1,3,5-O-tricaffeoylquinic acid, and 1,3,4-O-tricaffeoylquinic acid), and tetra Caffeoylquinic acid (1,3,4,5-O-tetracaffeo Lucina acid), and the like.

  A caffeoylquinic acid compound, which is a kind of polyphenol compound, has been reported to have various physiological activities such as prevention of dementia, anti-obesity, anti-hyperglycemia, anti-hypertension, anti-tumor, antibacterial and the like. In the method for producing a plant of the present invention, when a plant containing a caffeoylquinic acid compound is used as the polyphenol compound-containing plant, the plant of the caffeoylquinic acid compound having various physiological activities as described above is used. The content in the body can be significantly increased.

In the plant production method of the present invention, when a plant containing tricaffeoylquinic acid is used as the polyphenol compound-containing plant, the content of tricaffeoylquinic acid in the plant is more prominent. Can be increased.
Tricaffeoylquinic acid is a kind of caffeoylquinic acid compound, and among caffeoylquinic acid compounds, it is known to have particularly high physiological activity. However, no plant rich in tricaffeoylquinic acid has been found so far. Tricaffeoylquinic acid itself is a very rare substance. The plant production method of the present invention also has a remarkable effect that a plant containing tricaffeoylquinic acid at a high concentration can be produced as compared with existing tricaffeoylquinic acid-containing plants. obtain.
Hereinafter, the plant production method of the present invention will be described in detail for each step.

<Root removal process>
The root removal step is a step of removing the root of the grown polyphenol compound-containing plant. The term “growth” as used herein refers to a state in which the plant body is grown by a normal breeding method carried out for each plant type, variety, etc., and the plant body is sufficiently large. Means state. For example, in the case of sweet potato, it means a state in which the ground part of the sweet potato has developed four leaves (four nodes) or more, or a state in which the ground part has extended to 20 cm or more. In the case of wormwood and spring chrysanthemum, it means that the above-ground part has grown to 10 cm or more. In the case of artichoke and lettuce, it means that 4 to 5 or more true leaves have come out. In the case of burdock, it means a state where 3 to 4 or more true leaves have come out. In the case of taro, it means that three or more true leaves have come out. In the case of potatoes, it means that the above-ground part has grown to 10 cm or more. In the case of yacon, it means that the above-ground part has grown to 10 cm or more. In the case of ensai, it means a state where the above-ground part has grown to 10 cm or more.

The growing method of the plant body to be used for the root removal step is not particularly limited, and a known growing method can be selected according to the kind of the plant. The plant body to be subjected to the root removal step may be grown by hydroponics or by soil culture. The plant body to be subjected to the root removal step can be grown by a growing method as described in the following document, for example.
Potato: “Making potatoes and sweet potatoes taught by farmers”, separate volume Contemporary Agriculture, Agricultural Welfare Society, October 2013 issue, pp92-96; Sweet Potato: the same magazine, pp152-175; Artichoke: Great vegetable growing dictionary (2014), Supervision Masaaki Hojo, Seibidou Publishing Co., Ltd., pp8-9; Shungiku: the same magazine, pp34-35; Lettuce: the same magazine, pp80-81; Burdock: the same magazine, pp168-169; 187; Ensai: pp. 212-213
From the viewpoint of more significantly increasing the content of polyphenol compounds in the plant after removing the root, the plant subjected to the root removal process should be grown under sufficient light irradiation in the presence of water and fertilizer. Is preferred.

In the root removal step, the method for removing the root part of the plant is not particularly limited as long as the root part and the stem part of the plant body are separated and the root part can be removed from the plant body. .
Examples of means for removing the root of the plant include means such as cutting with a blade or folding by hand. As a method for removing the root part of the plant body, a method of cutting the stem so as not to include the root part is generally mentioned. Since the cut location differs depending on the type of plant body, it cannot be defined unconditionally. From the viewpoint that the plant body can be harvested multiple times, in the root removal step, it is preferable that the side buds are sufficiently elongated from the main branch, and then the elongated portion is cut from the vicinity of the main branch.
As a preferable mode in the case of cutting the stem, for example, using a sharp pruning shears, a pruning knife, a sickle, a clipper, a chainsaw or the like, and having an inclination angle with respect to the stem or perpendicular to the stem. The aspect cut | disconnected is mentioned. When cutting a stem with an inclination angle, the inclination angle is not particularly specified.
In the method for producing a plant of the present invention, it is not an obstacle to obtain the effect of the present invention that a new root grows in the plant after the root removal step.

<Storage process>
A preservation | save process is a process of preserve | saving the plant body which removed the root part at 20 to 40 degreeC in presence of water for 24 hours or more. The term “in the presence of water” as used herein means that there is moisture in the environment where the plant body from which the root part has been removed is placed, and water can be absorbed if the plant body from which the root part has been removed can absorb water to such an extent that it does not wither. The space to include is also included. In the case of storing a plant from which roots have been removed in a space containing water vapor, the amount of water vapor in the space is not particularly limited as long as the amount of water vapor is maintained so that the plant is not withered. The amount of water vapor that can be maintained without dying the plant varies depending on the plant body, so it cannot be generally defined. In general, the lower limit of the amount of water vapor in the space for storing the plant from which the roots have been removed is 50% relative humidity at 30 ° C.
“Preservation” in the preservation step means maintaining the freshness to such an extent that the plant body from which the root portion has been removed does not die, and it is not necessary to grow it. In a preservation | save process, you may leave the plant body which removed the root part in the space containing water vapor | steam.

From the viewpoint of sufficiently maintaining the freshness of the plant body from which the root portion has been removed and significantly increasing the content of the polyphenol compound in the plant body, the preservation step is preferably at least part of the plant body from which the root portion has been removed, Including placing and storing in at least one of water or soil, and more preferably, placing and storing at least a part of the plant body from which the root has been removed in water and then placing and storing in the soil. Including.
Specific examples of the preservation method in the preservation step include a preservation method in which the end portion of the plant body from which the root portion is removed is immersed in water, and the end portion of the plant body from which the root portion has been removed is applied to the soil containing water. Preservation method to insert, preservation method to cover the end of the plant body from which the root part is removed with a sponge soaked in water, absorbent cotton, etc., sponge or absorbent cotton soaked in water or water in a sealed space, and root part The preservation method etc. which put the removed plant body are mentioned.

In a preservation | save process, when arrange | positioning at least one part of the plant body which removed the root part to water, the commercially available liquid fertilizer (for example, Hyponex (trademark) etc.) used for hydroponics etc. is added with respect to water. Also good. “Water” as used herein refers to tap water or water containing an amount of minerals contained in tap water and does not contain fertilizer.
In a preservation | save process, when arrange | positioning at least one part of the plant body which removed the root part to soil, you may add the fertilizer used for soil cultivation etc. with respect to soil. The “use soil” here means non-fertilized soil that does not contain fertilizer such as black soil, akadama soil, kanuma soil, pumice, vermiculite, peat moss and the like. In addition, you may use a commercial item for a fertilizer.

  From the viewpoint of remarkably increasing the content of polyphenol compounds in the plant body, in the preservation process, after the root has newly grown in the plant body from which the root part has been removed, no fertilizer is added or substantially fertilizer is included. It is preferable to store under low fertilization conditions. The term “low fertilizer conditions that do not substantially contain fertilizers” as used herein means fertilizer conditions that include fertilizers that are necessary to maintain the freshness of the plant and that do not contain the amount of fertilizers intended for growth. To do. The amount of fertilization under low fertilization conditions is preferably 1/5 or less of the amount of fertilizer used for normal cultivation, more preferably 1/10 or less, and even more preferably 1/50 or less. .

The storage period in the storage process is 24 hours or more. The upper limit of the storage period is not particularly limited. If the storage period is less than 24 hours, the polyphenol compound contained in the plant cannot be increased sufficiently. The storage period in the storage step is preferably 2 to 35 days.
In the preservation step, the preservation period arranged in water (or arranged in a space containing water vapor) is preferably 1 day (24 hours) to 14 days from the viewpoint of productivity and economy, and preferably 2 days to It is more preferably 14 days, and further preferably 3 days to 7 days.
In a preservation | save process, it is preferable to arrange | position at least one part of the plant body which removed the root part to water for 2 days-14 days from a viewpoint of producing efficiently the plant body in which content of the polyphenol compound markedly increased. After placing at least a part of the removed plant in water for 3 days to 7 days, it is preferably 1 day to 60 days, more preferably 3 days to 40 days, still more preferably 7 days to 30 days, and particularly preferably 14 days. Place on the soil for a day to 21 days.

It is preferable that a preservation | save process includes irradiating light to the plant body from which the root part was removed from a viewpoint which increases content of the polyphenol compound in a plant body notably.
The light source for irradiating the plant from which the root is removed is not particularly limited, and may be sunlight, a fluorescent lamp, a xenon lamp, a mercury lamp, a halogen lamp, an LED (light-emitting diode), etc. An artificial light source may be used. When an LED is selected as the light source, a blue LED having a wavelength of 400 nm to 500 nm is preferably used from the viewpoint of increasing the content of the polyphenol compound in the plant body.
The light irradiation time per day is preferably 5 hours to 24 hours, more preferably 8 hours to 16 hours, and still more preferably 10 hours to 14 hours.
The illuminance of light in the light irradiation is not particularly limited. From the viewpoint of significantly increasing the content of the polyphenol compound in the plant body, the illuminance of light in the light irradiation is preferably 1,000 lux or more, and more preferably 10,000 lux or more.
The upper limit of the illuminance of light is not particularly limited as long as it does not adversely affect the plant by being too high, and may be set as desired. In general, the illuminance of light is desirably 200,000 lux or less.

  The preservation | save temperature in a preservation | save process is 20 to 40 degreeC from a viewpoint of increasing content of the polyphenol compound in a plant body. From the viewpoint of significantly increasing the content of the polyphenol compound in the plant body, the storage temperature in the storage step is preferably 20 ° C to 35 ° C.

<Other processes>
The plant body production method of the present invention may include other steps other than the root removal step and the storage step, if necessary, as long as the effects of the present invention are not impaired.

[Tricaffeoylquinic acid-containing plant]
The tricaffeoylquinic acid-containing plant of the present invention is produced by the above-described method for producing a plant of the present invention, and the content of tricaffeoylquinic acid is determined by drying the leaf blades of the tricaffeoylquinic acid-containing plant. The amount is preferably 80 mg or more, more preferably 200 mg or more, still more preferably 300 mg or more, and particularly preferably 500 mg or more per 100 g of mass.
To date, no plant rich in tricaffeoylquinic acid has been found.
In the method for producing a plant body of the present invention, a tricaffeoylquinic acid-containing plant body is used as the polyphenol compound-containing plant body, so that the tricaffeoylquinic acid-containing plant body is used. A plant containing quinic acid at a high concentration can be realized.

In the plant production method of the present invention, among the caffeoylquinic acid compounds contained in the plant body, the content of tricaffeoylquinic acid, which is a particularly rare substance having a high physiological activity, is remarkable. An increased plant body can be realized.
In the tricaffeoylquinic acid-containing plant that has undergone the root removal step and the preservation step in the plant production method of the present invention, the content of tricaffeoylquinic acid relative to the total amount of caffeoylquinic acid compound is used for the root removal step. Compared to the tricaffeoylquinic acid-containing plant, it is preferably 1.2 times or more, more preferably 2.0 times or more, still more preferably 3.0 times or more, and particularly preferably 3.5 times or more.
Since the example of a caffeoylquinic acid compound here is synonymous with the example of the caffeoylquinic acid compound demonstrated in the section of the production method of the plant of the present invention, explanation is omitted here.

  The content of tricaffeoylquinic acid in the plant can be confirmed by the following extraction method and quantitative method. Polyphenol compounds other than tricaffeoylquinic acid can also be extracted by the same method as the following extraction method, and by creating a calibration curve using a standard product of the polyphenol compound to be measured. Quantification can be performed by the same method as the following quantification method.

<Extraction method of tricaffeoylquinic acid>
The tricaffeoylquinic acid in the present invention is extracted using one of the following two methods.
1. The plant body is freeze-dried and then ground into a powder using a mill. A plant freeze-dried powder (50 mg) and 80 v / v% ethanol aqueous solution (5 mL) are placed in a 15-mL falcon tube and boiled for 5 minutes. After boiling, the ethanol aqueous solution containing the plant body cooled to 40 ° C. or lower is centrifuged at 3000 rpm (round per minute; the same applies hereinafter) for 15 minutes, and the resulting supernatant is recovered. The collected supernatant is filtered through a syringe filter having a pore size of 0.45 μm, and the obtained filtrate is used for the following quantitative analysis.
2. In a nitrogen atmosphere, 5 g of a raw plant cut into 1 cm to 2 cm and 50 mL of a 90 v / v% aqueous ethanol solution are placed in a 100 mL eggplant flask and heated at 80 ° C. for 1 hour. After the heating, the aqueous ethanol solution containing the plant body is filtered using absorbent cotton, and the filtrate (first filtrate) is recovered. The residue obtained by filtration is washed with 25 mL of a 90 v / v% aqueous ethanol solution, and the filtrate (second filtrate) is recovered. The first filtrate and the second filtrate are put into a 100 mL graduated cylinder, made up to 100 mL, filtered through a syringe filter having a pore size of 0.45 μm, and the obtained filtrate is used for the following quantitative analysis.

<Method of quantifying tricaffeoylquinic acid>
The tricaffeoylquinic acid in the present invention is analyzed using high performance liquid chromatography (HPLC) and quantified by an absolute calibration curve method. The HPLC analysis conditions are shown below.

-HPLC analysis conditions-
Measuring device: Prominence (liquid pump: LC-20AT, degasser: DGU-20A3, UV detector: SPD-20A, column oven: CTO-20AC, manufactured by Shimadzu Corporation)
Column: TSKgel ODS-100V 5 μm (inner diameter 4.6 mm × length 150 mm, manufactured by Tosoh Corporation)
Mobile phase: Liquid A) 0.2 v / v% formic acid-water, Liquid B) 0.2 v / v% formic acid-acetonitrile Gradient cycle: Liquid A + B liquid = 100%, showing the composition of liquid B.
B liquid = 2% → 19% (0 minutes → 16 minutes)
B liquid = 19% → 52% (16 minutes → 32 minutes)
Liquid B = 52% → 100% (32 minutes → 37 minutes)
B liquid = 100% → 2% (37 minutes → 47 minutes)
Flow rate: 1 mL / min Column temperature: 40 ° C
Injection volume: 10 μL
Detection wavelength: 330 nm

  The total amount of the polyphenol compound in the present invention can be measured by, for example, the Folin-Ciocalteu method described in Islam et al., J. Agric. Food Chem. 2002, 50, 3718-3722. The Folin-Ciocalteu method is a measurement method that utilizes the fact that a foreign reagent is colored by being reduced by a phenolic hydroxyl group. Specifically, the total amount of polyphenol compounds contained in the measurement sample is measured by mixing the measurement sample, the foreign reagent, and a 10% by mass aqueous sodium carbonate solution and measuring the absorbance at 600 nm. Note that chlorogenic acid (5-O-caffeoylquinic acid) can be used as the standard substance, and it may be calculated as a chlorogenic acid equivalent concentration.

[Method for increasing the amount of polyphenol compound in a polyphenol compound-containing plant]
The method for increasing the amount of polyphenol compound in the polyphenol compound-containing plant of the present invention (hereinafter also referred to as “polyphenol compound increase method”) is a group consisting of potatoes other than sweet potatoes, convolvulaceae plants, and asteraceae plants. The root part removing step for removing the root part of the plant body containing the polyphenol compound grown (hereinafter also referred to as “polyphenol compound-containing plant body”) selected from the above, and the plant body from which the root part has been removed, in the presence of water at 20 ° C. The preservation | save process preserve | saved for 24 hours or more at -40 degreeC.
In the method for increasing a polyphenol compound of the present invention, after removing the root of the grown polyphenol compound-containing plant, the plant from which the root has been removed is stored at 20 ° C. to 40 ° C. for 24 hours or more in the presence of water. By such a simple method, the amount of the polyphenol compound in the polyphenol compound-containing plant can be increased from the amount originally contained.
In the method for increasing the polyphenol compound of the present invention, the amount of the polyphenol compound is preferably 1.1 times or more, more preferably 1.3 times or more, still more preferably 1.4 times or more than the plant body to be subjected to the root removal step. Particularly preferably, it can be increased by 1.5 times or more.

The polyphenol compound-containing plant that can be applied to the method for increasing the polyphenol compound of the present invention is synonymous with the polyphenol compound-containing plant described in the section of the plant production method of the present invention, and preferred examples are also the same. The description is omitted here.
Further, the root removal step and the preservation step in the method for increasing the polyphenol compound of the present invention are also synonymous with the root removal step and the preservation step described in the section of the plant production method of the present invention, and preferred examples are also the same. Therefore, the description is omitted here.
The method for increasing the polyphenol compound of the present invention may include other steps than the root removal step and the storage step, as necessary, as long as the effects of the present invention are not impaired.

[Method for increasing the amount of caffeoylquinic acid compound in a plant containing caffeoylquinic acid compound]
The method of increasing the amount of caffeoylquinic acid compound in the caffeoylquinic acid compound-containing plant of the present invention (hereinafter also referred to as “caffeoylquinic acid compound increasing method”) is a potato other than sweet potato, Convolvulaceae. A root removal step for removing the root of a plant and a plant containing a caffeoylquinic acid compound (hereinafter also referred to as “caffeoylquinic acid compound-containing plant”) selected from the group consisting of plants and asteraceae plants; And a preservation step of preserving the plant from which the root has been removed at 20 ° C. to 40 ° C. for 24 hours or more in the presence of water.
A caffeoylquinic acid compound is a substance having high physiological activity among polyphenol compounds.
In the method for increasing the caffeoylquinic acid compound of the present invention, after removing the root of the grown caffeoylquinic acid compound-containing plant, the plant from which the root has been removed is removed at 20 ° C. to 40 ° C. in the presence of water. The amount of caffeoylquinic acid compound in the caffeoylquinic acid compound-containing plant can be increased from the amount originally contained by a simple method of storing for 24 hours or more.
In the method for increasing caffeoylquinic acid compound of the present invention, the amount of caffeoylquinic acid compound is preferably 1.2 times or more, more preferably 1.5 times or more, and still more preferably than the plant body subjected to the root removal step. It can be increased by 2.0 times or more, particularly preferably 2.4 times or more.

The caffeoylquinic acid compound-containing plant that can be applied to the method for increasing the caffeoylquinic acid compound of the present invention is synonymous with the caffeoylquinic acid compound-containing plant described in the section of the plant production method of the present invention. Since a preferable example is the same, description is abbreviate | omitted here.
Further, the root removal step and the preservation step in the method for increasing the caffeoylquinic acid compound of the present invention are also synonymous with the root removal step and the preservation step described in the section of the plant production method of the present invention, and preferred examples are also included. Since it is the same, description is abbreviate | omitted here.
The method for increasing the caffeoylquinic acid compound of the present invention may include other steps other than the root removal step and the storage step, if necessary, as long as the effects of the present invention are not impaired.

[Method for increasing the amount of tricaffeoylquinic acid in tricaffeoylquinic acid-containing plants]
The method for increasing the amount of tricaffeoylquinic acid in the tricaffeoylquinic acid-containing plant according to the present invention (hereinafter also referred to as “method for increasing tricaffeoylquinic acid”) is a potato other than sweet potato, Convolvulaceae. A root removal step for removing the root of a plant containing tricaffeoylquinic acid (hereinafter also simply referred to as “plant”) selected from the group consisting of plants and asteraceae, and a plant from which the root has been removed The preservation | save process which preserve | saves a body for 24 hours or more at 20 degreeC-40 degreeC in presence of water.
Tricaffeoylquinic acid is a very rare substance having a particularly high physiological activity among polyphenol compounds. However, the amount of tricaffeoylquinic acid in existing tricaffeoylquinic acid-containing plants is very small, and no plant body rich in tricaffeoylquinic acid has been found.
In the method for increasing tricaffeoylquinic acid according to the present invention, after removing the root of the grown tricaffeoylquinic acid-containing plant, the plant from which the root has been removed is removed at 20 ° C. to 40 ° C. in the presence of water. By the simple method of storing for 24 hours or more, the amount of tricaffeoylquinic acid in the tricaffeoylquinic acid-containing plant can be increased from the amount originally contained.
In the method for increasing tricaffeoylquinic acid of the present invention, the amount of tricaffeoylquinic acid is preferably 1.2 times or more, more preferably 2.0 times or more, and still more preferably than the plant body to be subjected to the root removal step. It can be increased by 3.0 times or more, particularly preferably by 3.5 times or more.

The tricaffeoylquinic acid-containing plant that can be applied to the method for increasing tricaffeoylquinic acid of the present invention is synonymous with the tricaffeoylquinic acid-containing plant described in the section of the plant production method of the present invention. Since a preferable example is the same, description is abbreviate | omitted here.
Further, the root removal step and the preservation step in the method for increasing tricaffeoylquinic acid of the present invention are also synonymous with the root removal step and the preservation step described in the section of the plant production method of the present invention, and preferred examples are also included. Since it is the same, description is abbreviate | omitted here.
The method for increasing tricaffeoylquinic acid of the present invention may include other steps other than the root removal step and the storage step, if necessary, as long as the effects of the present invention are not impaired.

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to the following examples unless it exceeds the gist thereof.
In the examples, the abbreviations shown in Table 1 were used as the compound names.

In Examples, 3-CQA, 4-CQA, and 5-CQA are collectively referred to as “chlorogenic acid (ChA)”.
In the examples, 3,4-DCQA, 3,5-DCQA, and 4,5-DCQA are collectively referred to as “DCQA”.
In the examples, “total amount of caffeoylquinic acid compound” refers to 3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA. It means the total amount.

[Extraction method of polyphenol compound]
In Examples, polyphenol compounds (isoquercetin, 3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA) contained in the plant are Extraction was performed by the following method.

1. Extraction of polyphenol compound from freeze-dried plant The freeze-dried plant was pulverized into powder using a mill. 50 mg of the lyophilized powder of the obtained plant and 5 mL of 80 v / v% aqueous ethanol solution were placed in a 15 mL falcon tube and boiled for 5 minutes. After boiling, the aqueous ethanol solution containing the plant body cooled to 40 ° C. or lower was centrifuged at 3000 rpm for 15 minutes, and the resulting supernatant was collected. The collected supernatant was filtered through a syringe filter having a pore size of 0.45 μm to obtain a filtrate. The obtained filtrate was used for the following quantitative analysis.

2. Extraction of Polyphenol Compound from Raw Plant Body Under a nitrogen atmosphere, 5 g of a plant body cut to 1 cm to 2 cm and 50 mL of a 90 v / v% aqueous ethanol solution were placed in a 100 mL volume eggplant flask and heated at 80 ° C. for 1 hour. After the heating, the ethanol aqueous solution containing the plant body was filtered using absorbent cotton, and the filtrate (first filtrate) was recovered. The residue obtained by filtration was washed with 25 mL of a 90 v / v% aqueous ethanol solution, and the filtrate (second filtrate) was recovered. The first filtrate and the second filtrate were placed in a 100 mL capacity measuring cylinder and made up to 100 mL, and then filtered through a syringe filter having a pore size of 0.45 μm to obtain a filtrate. The obtained filtrate was used for the following quantitative analysis.

[Quantification method of each polyphenol compound]
In Examples, quantification of polyphenol compounds (isoquercetin, 3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA) contained in plants. Were performed by an absolute calibration curve method using high performance liquid chromatography (HPLC). The HPLC analysis conditions are shown below.

-HPLC analysis conditions-
Measuring device: Prominence (liquid pump: LC-20AT, degasser: DGU-20A3, UV detector: SPD-20A, column oven: CTO-20AC, manufactured by Shimadzu Corporation)
Column: TSKgel ODS-100V 5 μm (inner diameter 4.6 mm × length 150 mm, manufactured by Tosoh Corporation)
Mobile phase: Liquid A) 0.2 v / v% formic acid-water, Liquid B) 0.2 v / v% formic acid-acetonitrile Gradient cycle: Liquid A + B liquid = 100%, showing the composition of liquid B.
B liquid = 2% → 19% (0 minutes → 16 minutes)
B liquid = 19% → 52% (16 minutes → 32 minutes)
Liquid B = 52% → 100% (32 minutes → 37 minutes) Liquid B = 100% → 2% (37 minutes → 47 minutes)
Flow rate: 1 mL / min Column temperature: 40 ° C
Injection volume: 10 μL
Detection wavelength: 330 nm

[Measurement method of total amount of polyphenol compound]
In the examples, the total amount of the polyphenol compound contained in the plant was measured by the Folin-Ciocalteu method. The filtrate obtained above, the foreign reagent, and a 10% by mass aqueous sodium carbonate solution were mixed to obtain a measurement sample. The absorbance of the obtained measurement sample at 600 nm was measured using a microplate reader (SpectraMax Plus 384, manufactured by Molecular Devices). As a standard substance, chlorogenic acid (5-O-caffeoylquinic acid) was used, and a calibration curve for the standard substance was prepared for each measurement. The total amount of polyphenol compounds contained in the plant body was calculated as a chlorogenic acid equivalent concentration by the prepared calibration curve.
In the tables of the examples, “total amount of polyphenol compound” is expressed as “total polyphenol”.

<Test Example 1>
Soil cultivation was carried out in an outdoor house (under sunlight), and the stems and leaves of the eight sweet potatoes grown were cut and harvested so as not to include the roots. The harvested sweet potato stalks and leaves are made into a 1000-fold diluted solution of Hyponex (registered trademark) (NPK = 6-10-5) so that the foliage does not fade as in the case of maintaining the freshness of flowers. It was inserted so that the cut end was immersed and the leaves were not immersed.
Sweet potato stems and leaves inserted into this Hyponex (registered trademark) were placed in an artificial meteorometer (temperature: 30 ° C., relative humidity: 70%, illuminance: 23,000 lux, 12-hour day length (light period: 6:00 to 18:00, dark) (Period: 18:00 to 6:00))) and stored for 7 days.
Sweet potato leaves immediately after harvest (0 days of storage), 3 days of harvested sweet potato leaves, and 7 days of harvested sweet potato leaves were lyophilized to extract polyphenol compounds, and then sweet potato The total amount of polyphenol compound contained in the leaf blades was measured, and 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA were quantified. The measurement was performed twice by changing the harvested part of the sweet potato leaf, and the respective measurement samples were referred to as measurement sample A and measurement sample B. The measurement results are shown in Tables 2 to 5. The unit of the total amount of polyphenols in Tables 2 and 3 is g ChA equivalent / 100 g F.I. D. P. The unit of the content of each polyphenol compound in Table 4 and Table 5 is g / 100 g F. D. P. (Leaf lyophilized powder).

As shown in Tables 2 and 3, the total amount of polyphenols contained in the leaves of sweet potato increased steadily.
On the other hand, although not shown in the table, when the above-grown sweet potato was grown in soil without cutting the foliage for 7 days, there was almost no change in the total amount of polyphenols contained in the leaves of the sweet potato. I couldn't see it.

Further, as shown in Tables 4 and 5, the amounts of 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA contained in the sweet potato leaf blades increased steadily.
On the other hand, although not shown in the table, when the above-grown sweet potato was grown on the soil for 7 days without cutting the foliage, 3,4-DCQA contained in the leaf of sweet potato, 3, 5 -Little change was seen in the amount of DCQA, 4,5-DCQA, and TCQA.

<Test Example 2>
Soil cultivation was carried out in an outdoor house (under sunlight), and the stems and leaves of the eight sweet potatoes grown were cut and harvested so as not to include the roots. The harvested sweet potato stalks and leaves are made into a 1000-fold diluted solution of Hyponex (registered trademark) (NPK = 6-10-5) so that the foliage does not fade as in the case of maintaining the freshness of flowers. It was inserted so that the cut end was immersed and the leaves were not immersed.
The stems and leaves of sweet potatoes inserted in this Hyponex (registered trademark) were stored for 7 days in an artificial weather apparatus (temperature: 30 ° C., relative humidity: 70%, non-illuminated).
Sweet potato leaves immediately after harvest (0 days of storage), 3 days of harvested sweet potato leaves, and 7 days of harvested sweet potato leaves were lyophilized to extract polyphenol compounds, and then sweet potato The total amount of polyphenol compounds contained in the leaf blades was measured. Table 6 shows the measurement results. The unit of the total amount of polyphenols in Table 6 is g ChA equivalent / 100 g F.I. D. P. (Leaf lyophilized powder).

  As shown in Table 6, the total amount of the polyphenol compound contained in the leaf of the sweet potato increased steadily under no lighting conditions.

<Test Example 3>
Soil cultivation was carried out in an outdoor house (under sunlight), and the stems and leaves of the eight sweet potatoes grown were cut and harvested so as not to include the roots. The harvested sweet potato stalks and leaves were inserted in tap water so that the foliage was not soaked and the leaves were not soaked in tap water so that the foliage would not withstand, as in the case of maintaining the freshness of flowers.
The stems and leaves of sweet potatoes inserted in this tap water are stored in a storage room (temperature: 30 ° C., relative humidity: 70%, illuminance: 2,000 lux, 12 hours long day (light period: 6:00 to 18:00, dark period: 18:00) -6))) and stored for 3 days.
Sweet potato leaves immediately after harvest (0 days of storage) and sweet potato leaves harvested after storage for 3 days were freeze-dried, polyphenol compounds were extracted, and TCQA contained in the sweet potato leaves was quantified. . Table 7 shows the measurement results. The unit of TCQA content in Table 7 is g / 100 g F.I. D. P. (Leaf lyophilized powder).

  As shown in Table 7, TCQA contained in the leaves of sweet potato increased smoothly under the illuminance condition of 2,000 lux.

<Test Example 4>
Cultivate soil in an outdoor house (under sunlight) and grow 8 shoots of sweet potato grown (variety: Koganesengan, a different varieties from the sweet potato used in Test Example 1) so as not to include the roots. Harvested. The harvested sweet potato stalks and leaves are made into a 1000-fold diluted solution of Hyponex (registered trademark) (NPK = 6-10-5) so that the foliage does not fade as in the case of maintaining the freshness of flowers. It was inserted so that the cut end was immersed and the leaves were not immersed.
Sweet potato stems and leaves inserted into this Hyponex (registered trademark) were placed in an artificial meteorometer (temperature: 30 ° C., relative humidity: 70%, illuminance: 23,000 lux, 12-hour day length (light period: 6:00 to 18:00, dark) (Period: 18:00 to 6:00))) and stored for 7 days.
Sweet potato leaves immediately after harvest (0 days of storage), 3 days of harvested sweet potato leaves, and 7 days of harvested sweet potato leaves were lyophilized to extract polyphenol compounds, and then sweet potato The total amount of polyphenol compound contained in the leaf blades was measured, and 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA were quantified. The measurement results are shown in Tables 8 to 10. The unit of the total amount of polyphenols in Table 8 is g ChA equivalent / 100 g F.I. D. P. The unit of the content of each polyphenol compound in Table 9 and Table 10 is g / 100 g F. D. P. (Leaf lyophilized powder).

As shown in Table 8, the total amount of polyphenol compounds contained in the leaves of sweet potato (variety: Koganesengan) increased steadily.
In addition, as shown in Tables 9 and 10, the amounts of 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA contained in the leaves of sweet potato (variety: Koganesengan) Increased.

<Test Example 5>
Cultivate soil in an outdoor house (under sunlight) and grow the stems and leaves of 8 nodes of the grown sweet potato (variety: Murasakikimari, a variety different from the sweet potato used in Test Example 1) so as not to include the roots. Harvested. The harvested sweet potato stalks and leaves are made into a 1000-fold diluted solution of Hyponex (registered trademark) (NPK = 6-10-5) so that the foliage does not fade as in the case of maintaining the freshness of flowers. It was inserted so that the cut end was immersed and the leaves were not immersed.
Sweet potato stems and leaves inserted into this Hyponex (registered trademark) were placed in an artificial meteorometer (temperature: 30 ° C., relative humidity: 70%, illuminance: 23,000 lux, 12-hour day length (light period: 6:00 to 18:00, dark) Period: 18:00 to 6:00))) and stored for 3 days.
Sweet potato leaves immediately after harvest (0 days of storage) and potato leaves harvested after storage for 3 days are freeze-dried, polyphenol compounds are extracted, and caffeoylquinic acid compounds ( 3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA) were quantified. The measurement results are shown in Tables 11 and 12. In Table 11, the unit of content of 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA, and the unit of total amount of caffeoylquinic acid compound in Table 12 are all g / 100 g F.I. D. P. (Leaf lyophilized powder).

  As shown in Tables 11 and 12, the amounts of 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA contained in the leaf blades of sweet potato (variety: Murasakimasari), and caffeoylquinic acid The total amount of compound increased steadily.

<Test Example 6>
Cultivate soil in an outdoor house (under sunlight), and cut the stems and leaves of 8 nodes of the grown sweet potato (variety: Tama Akane, a different varieties from the sweet potato used in Test Example 1) so as not to include the root part, Harvested. The harvested sweet potato stalks and leaves are made into a 1000-fold diluted solution of Hyponex (registered trademark) (NPK = 6-10-5) so that the foliage does not fade as in the case of maintaining the freshness of flowers. It was inserted so that the cut end was immersed and the leaves were not immersed.
Sweet potato stems and leaves inserted into this Hyponex (registered trademark) were placed in an artificial meteorometer (temperature: 30 ° C., relative humidity: 70%, illuminance: 23,000 lux, 12-hour day length (light period: 6:00 to 18:00, dark) (Period: 18:00 to 6:00))) and stored for 7 days.
Sweet potato leaves immediately after harvest (day 0) and potato leaves harvested after storage for 7 days are freeze-dried, polyphenol compounds are extracted, and caffeoylquinic acid compounds contained in the leaves of sweet potatoes ( 3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA) were quantified. The measurement results are shown in Table 13 and Table 14. In addition, the unit of the content of DCQA (3,4-DCQA, 3,5-DCQA, and 4,5-DCQA) and TCQA in Table 13 and the unit of the total amount of caffeoylquinic acid compound in Table 14 are G / 100 g F.I. D. P. (Leaf lyophilized powder).

  As shown in Table 13 and Table 14, the amount of DCQA and TCQA contained in the leaf blades of sweet potato (variety: Tamaakane) and the total amount of caffeoylquinic acid compound increased steadily.

<Test Example 7>
The stems and leaves of 8 nodes of the sweet potato grown in an outdoor house (under sunlight) and grown (variety: Kokei No. 14, different varieties from the sweet potato used in Test Example 1) should not include the roots. Cut and harvested. The harvested sweet potato stalks and leaves are made into a 1000-fold diluted solution of Hyponex (registered trademark) (NPK = 6-10-5) so that the foliage does not fade as in the case of maintaining the freshness of flowers. It was inserted so that the cut end was immersed and the leaves were not immersed.
Sweet potato stems and leaves inserted into this Hyponex (registered trademark) were placed in an artificial meteorometer (temperature: 30 ° C., relative humidity: 70%, illuminance: 23,000 lux, 12-hour day length (light period: 6:00 to 18:00, dark) Period: 18:00 to 6:00))) and stored for 3 days.
Sweet potato leaves immediately after harvest (0 days of storage) and potato leaves harvested after storage for 3 days are freeze-dried, polyphenol compounds are extracted, and caffeoylquinic acid compounds ( 3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA) were quantified. The measurement results are shown in Table 15 and Table 16. In addition, the unit of the content of DCQA (3,4-DCQA, 3,5-DCQA, and 4,5-DCQA) and TCQA in Table 15 and the unit of the total amount of caffeoylquinic acid compound in Table 16 are G / 100 g F.I. D. P. (Leaf lyophilized powder).

  As shown in Table 15 and Table 16, the amount of DCQA and TCQA contained in the leaf blades of sweet potato (variety: No. 14) and the total amount of caffeoylquinic acid compound increased steadily.

<Test Example 8>
8 bunches of sweet potato (variety: white yutaka, different from the sweet potato used in Test Example 1) cultivated under indoor fluorescent light (fluorescent light for plant growth Biolux (registered trademark) A, NEC Lighting) The foliage was cut and removed so as not to include the roots. The room was adjusted to have an air temperature of 30 ° C. and a relative humidity of 60%. The illuminance of the fluorescent lamp was adjusted so that the tip of the sweet potato seedling was about 6,000 lux. The fluorescent lamp was turned on and off at a cycle of 12 hours.
The harvested sweet potato stalks and leaves are made into a 1000-fold diluted solution of Hyponex (registered trademark) (NPK = 6-10-5) so that the foliage does not fade as in the case of maintaining the freshness of flowers. It was inserted so that the cut end was immersed and the leaves were not immersed. The sweet potato stems and leaves inserted in this hyponex solution were stored for 4 days under the same indoor environment as described above.
We harvested the leaves of sweet potato just after harvesting (0 days of storage) and the leaves of sweet potatoes harvested after storage for 4 days, and extracted polyphenol compounds from each of the harvested raw leaves to make the leaves of sweet potatoes Quantification of TCQA and isoquercetin contained was performed. Table 17 shows the measurement results. In addition, the unit of content of TCQA and isoquercetin in Table 17 is g / 100 g D.E. P. The dry leaf blade mass was converted to the dry leaf mass using the conversion factor calculated by the following method.
A portion of the raw leaf blade used for extraction was sampled, and dried under reduced pressure at 80 ° C. using a vacuum pump until the weight became constant. Based on the mass change before and after drying, a coefficient (conversion coefficient) for converting the mass of raw leaf blades to the mass of dried leaf blades was calculated.

  As shown in Table 17, the amounts of TCQA and isoquercetin contained in the leaf blades of sweet potato (variety: Shiroyutaka) both increased steadily.

<Test Example 9>
Cut the stems and leaves of 8 nodes of Ensai (Convolvulaceae) cultivated under indoor fluorescent lamps (fluorescent lamps for plant growth Biolux (registered trademark) A, NEC Lighting) so as not to include roots, Harvested. The room was adjusted to have an air temperature of 30 ° C. and a relative humidity of 60%. The illuminance of the fluorescent lamp was adjusted so that the tip of the seedling of ensai was about 6,000 lux. The fluorescent lamp was turned on and off at a cycle of 12 hours.
In the same manner as when maintaining the freshness of flowers, the stems and leaves of the harvested ensai were added to a 1000-fold diluted solution of Hyponex (registered trademark) (NPK = 6-10-5) so that the stems and leaves do not fade. It was inserted so that the cut end was immersed and the leaves were not immersed. The foliage of Ensai inserted in this Hyponex solution was stored for 2 days under the same indoor environment as described above.
Harvesting the leaves of Ensai immediately after harvest (0 days of storage) and the leaves of Ensai that were stored for 2 days and harvested, and extracted polyphenol compounds from each of the harvested raw leaves, to the leaves of Ensai The contained caffeoylquinic acid compounds (3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA) were quantified. The measurement results are shown in Table 18. In Table 18, the unit of the content of ChA (3-CQA, 4-CQA, and 5-CQA), DCQA (3,4-DCQA, 3,5-DCQA, and 4,5-DCQA), and TCQA G / 100 g D.I. P. Using the conversion factor calculated by the same method as in Test Example 8, the raw leaf mass was converted to the dry leaf mass.

  As shown in Table 18, the amount of caffeoylquinic acid compounds (ChA, DCQA, and TCQA) contained in the leaves of Ensai increased steadily.

<Test Example 10>
Cut the stems and leaves (15 cm tip) of spring chrysanthemum (Asteraceae) cultivated under indoor fluorescent lamps (fluorescent lamps for plant growth, Biolux (registered trademark) A, NEC Lighting) so as not to include the roots. And harvested. The interior of the room was adjusted to a temperature of 30 ° C. Moreover, the indoor humidity was adjusted so as to have a saturated vapor pressure at an air temperature of 30 ° C. The illuminance of the fluorescent lamp was adjusted so that the tip of the spring chrysanthemum seedling was about 6,000 lux. The fluorescent lamp was turned on and off at a cycle of 12 hours.
In the same way as when maintaining the freshness of flowers, the stems and leaves of the harvested spring chrysanthemum are diluted into a 1000-fold diluted solution of Hyponex (registered trademark) (NPK = 6-10-5) so that the stems and leaves do not fade. It was inserted so that the cut end was immersed and the leaves were not immersed. Crunchyme stems and leaves inserted in this hyponex solution were stored for 2 days in the same indoor environment as described above.
Harvested spring chrysanthemum leaves immediately after harvest (0 days of storage) and harvested spring chrysanthemum leaves preserved for 2 days, and extracted polyphenolic compounds from each harvested raw leaf blades, into spring chrysanthemum leaves The contained caffeoylquinic acid compounds (3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and TCQA) were quantified. The measurement results are shown in Table 19. In Table 19, the unit of content of ChA (3-CQA, 4-CQA, and 5-CQA), DCQA (3,4-DCQA, 3,5-DCQA, and 4,5-DCQA), and TCQA G / 100 g D.I. P. Using the conversion factor calculated by the same method as in Test Example 8, the raw leaf mass was converted to the dry leaf mass.

  As shown in Table 19, the amount of caffeoylquinic acid compounds (ChA, DCQA, and TCQA) contained in the leaves of spring chrysanthemum increased smoothly.

<Test Example 11>
The stems and leaves of 4 nodes of sweet potato cultivated in soil under an indoor fluorescent lamp (fluorescent lamp for plant growth Biolux (registered trademark) A, NEC Lighting) were cut and harvested so as not to include roots. The room was adjusted to have an air temperature of 30 ° C. and a relative humidity of 60%. The illuminance of the fluorescent lamp was adjusted so that the tip of the sweet potato seedling was about 6,000 lux. The fluorescent lamp was turned on and off at a cycle of 12 hours.
Polyphenol compounds are extracted from the harvested raw leaf blades, and caffeoylquinic acid compounds (3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA) contained in the leaves of sweet potato , 4,5-DCQA, and TCQA). Table 20 shows the measurement results. In Table 20, the contents of ChA (3-CQA, 4-CQA, and 5-CQA), DCQA (3,4-DCQA, 3,5-DCQA, and 4,5-DCQA), and TCQA, and The unit of the total amount of caffeoylquinic acid compound is g / 100 g D.E. P. Using the conversion factor calculated by the same method as in Test Example 8, the raw leaf mass was converted to the dry leaf mass.

<Test Example 12>
The stems and leaves of 4 nodes of sweet potato cultivated in soil under an indoor fluorescent lamp (fluorescent lamp for plant growth Biolux (registered trademark) A, NEC Lighting) were cut and harvested so as not to include roots. The room was adjusted to have an air temperature of 30 ° C. and a relative humidity of 60%. The illuminance of the fluorescent lamp was adjusted so that the tip of the sweet potato seedling was about 6,000 lux. The fluorescent lamp was turned on and off at a cycle of 12 hours.
The harvested sweet potato stalks and leaves were inserted in water so that the cut end was immersed and the leaves were not immersed. The potato stems and leaves inserted in this water were stored for 6 days under the same indoor environment as above. The stems and leaves of the sweet potato preserved for 6 days were planted in vermiculite (non-fertilized soil) and stored for another 15 days under the same indoor environment as described above, while harvesting the leaf blades.
In addition, as a non-fertile soil, instead of vermiculite, black soil, akadama soil, or pumice stone was used as a non-fertile soil, and the harvested sweet potato stems and leaves were preserved in the same indoor environment as described above, and then the leaf blades were harvested. . Table 21 shows the storage period in each system.

  Polyphenol compounds are extracted from the harvested raw leaf blades, and caffeoylquinic acid compounds (3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA) contained in the leaves of sweet potato , 4,5-DCQA, and TCQA). The measurement results are shown in Table 22 and Table 23 (relative values with respect to Test Example 11). In Table 22, the contents of ChA (3-CQA, 4-CQA, and 5-CQA), DCQA (3,4-DCQA, 3,5-DCQA, and 4,5-DCQA), and TCQA, and The unit of the total amount of caffeoylquinic acid compound is g / 100 g D.E. P. Using the conversion factor calculated by the same method as in Test Example 8, the raw leaf mass was converted to the dry leaf mass.

  As shown in Table 22 and Table 23, the content of the caffeoylquinic acid compounds (ChA, DCQA, and TCQA) is increased in all systems as compared to Test Example 11 (0 days of storage, immediately after harvesting). It was confirmed. Further, among the caffeoylquinic acid compounds, the increase in TCQA content was particularly remarkable.

<Test Example 13>
The stems and leaves of 4 nodes of sweet potato cultivated in soil under an indoor fluorescent lamp (fluorescent lamp for plant growth Biolux (registered trademark) A, NEC Lighting) were cut and harvested so as not to include roots. The room was adjusted to have an air temperature of 30 ° C. and a relative humidity of 60%. The illuminance of the fluorescent lamp was adjusted so that the tip of the sweet potato seedling was about 10,000 lux. The fluorescent lamp was turned on and off at a cycle of 12 hours.
The harvested sweet potato stems and leaves were put in a plastic bag and sealed as they were. Absorbent cotton moistened with water was placed in the plastic bag so that water vapor was present. And after storing for 7 days under the same indoor environment as described above, the leaf blades were harvested.
Polyphenol compounds are extracted from the harvested raw leaf blades, and caffeoylquinic acid compounds (3-CQA, 4-CQA, 5-CQA, 3,4-DCQA, 3,5-DCQA) contained in the leaves of sweet potato , 4,5-DCQA, and TCQA). The measurement results are shown in Table 24 and Table 25 (relative values with respect to Test Example 11). In Table 24, the contents of ChA (3-CQA, 4-CQA, and 5-CQA), DCQA (3,4-DCQA, 3,5-DCQA, and 4,5-DCQA), and TCQA, and The unit of the total amount of caffeoylquinic acid compound is g / 100 g D.E. P. Using the conversion factor calculated by the same method as in Test Example 8, the raw leaf mass was converted to the dry leaf mass.

  As shown in Table 24 and Table 25, it was confirmed that the content of caffeoylquinic acid compounds (ChA, DCQA, and TCQA) increased as compared with Test Example 11 (0 days of storage, immediately after harvesting). . Further, among the caffeoylquinic acid compounds, the increase in TCQA content was particularly remarkable.

Claims (15)

A root removal step for removing the root of a plant body containing a grown polyphenol compound selected from the group consisting of potatoes other than sweet potato, convolvulaceae, and asteraceae,
A preservation step of storing the plant from which the root has been removed at 20 ° C. to 40 ° C. in the presence of water for 24 hours or more,
A method for producing a polyphenol compound-containing plant in which the amount of the polyphenol compound is increased as compared with the plant subjected to the root removal step. The polyphenol compound-containing plant that removes the root in the root removal step is a caffeoylquinic acid compound-containing plant,
The production method according to claim 1, wherein the amount of the caffeoylquinic acid compound is increased as compared with the plant body containing the caffeoylquinic acid compound used in the root removal step. The polyphenol compound-containing plant that removes the root in the root removal step is a tricaffeoylquinic acid-containing plant,
The production method according to claim 1, wherein the amount of tricaffeoylquinic acid is increased as compared to a tricaffeoylquinic acid-containing plant used for the root removal step.   The production method according to any one of claims 1 to 3, wherein a preservation period in the preservation step is 2 days to 35 days.   The production method according to any one of claims 1 to 4, wherein a preservation period in the preservation step is 3 days to 7 days.   The said preservation | save process is a production method of any one of Claims 1-5 including irradiating light to the plant body from which the said root part was removed.   The production method according to claim 6, wherein an illuminance of light in the light irradiation is 1,000 lux or more.   The production method according to any one of claims 1 to 7, wherein a storage temperature in the storage step is 20 ° C to 35 ° C.   The production method according to any one of claims 1 to 8, wherein the storing step includes storing at least a part of the plant body from which the root portion has been removed, arranged in at least one of water and soil. .   The said preservation | save process includes arrange | positioning and preserve | saving at least one part of the plant body from which the said root part was removed, arrange | positioning in water, and storing it in any one of Claims 1-9. The production method described.   The production method according to any one of claims 1 to 10, wherein the plant body is a convolvulaceae plant.   The production method according to claim 11, wherein the convolvulaceae plant is sweet potato.   It is produced by the production method according to any one of claims 3 to 12, and the content of tricaffeoylquinic acid is 80 mg or more per 100 g of dry mass of the leaf blade of the tricaffeoylquinic acid-containing plant. A tricaffeoylquinic acid-containing plant. A root removal step for removing the root of a plant body containing a grown polyphenol compound selected from the group consisting of potatoes other than sweet potato, convolvulaceae, and asteraceae,
A preservation step of storing the plant from which the root has been removed at 20 ° C. to 40 ° C. in the presence of water for 24 hours or more,
A method for increasing the amount of a polyphenol compound in a polyphenol compound-containing plant. A root removal step for removing the roots of the grown tricaffeoylquinic acid-containing plant selected from the group consisting of potatoes other than sweet potatoes, convolvulaceae and asteraceae,
A preservation step of storing the plant from which the root has been removed at 20 ° C. to 40 ° C. in the presence of water for 24 hours or more,
A method for increasing the amount of tricaffeoylquinic acid in a tricaffeoylquinic acid-containing plant.