JP2016094372A - Composition for high carotenoid absorption - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition for high absorption of carotenoid which is hydrophobic, the composition enhancing carotenoid absorption into a living body and making efficiently obtaining carotenoid physiology effect with a little intake easy.SOLUTION: The invention provides a composition for high carotenoid absorption containing deep ocean water and carotenoid. Preferably, the carotenoid is derived from Citreae, more preferably, the carotenoid derived from Citreae is cryptoxanthin, and still more preferably, the deep ocean water is raw water or a mixed water of a desalted water obtained by a reverse osmosis membrane method and the raw water of the deep ocean water.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a carotenoid superabsorbent composition, more specifically a carotenoid superabsorbent composition containing deep ocean water and carotenoid, and is used in foods and drinks, pharmaceuticals, quasi drugs, cosmetics, feeds, pet foods, etc. Can be used.

  Carotenoid is a pigment contained in animals and plants, microorganisms, etc., and is a general term for molecules having a specific skeleton. As its functionality, provitamin A activity, antioxidant action (for example, see Non-Patent Document 1), anti-obesity action (for example, see Non-Patent Document 2), skin beautifying action (for example, see Patent Document 1). However, its properties belong to hydrophobicity, and it is difficult to say that the absorption to the living body is sufficient for the intake. Therefore, ingestion of an effective amount that exhibits the above-mentioned functionality requires a large amount of intake, but such an intake form is not only economically wasteful but also has a concern of adversely affecting the living body. . For this reason, various studies have been made in order to improve the absorbability. For example, combined use with an emulsifier has been studied (for example, see Patent Document 2).

  However, even when used in combination with an emulsifier, the absorption promotion action of the carotenoid obtained is limited, and satisfactory results have not been obtained. In addition, there is a concern that the number of steps increases due to the combined use with an emulsifier, and the costs associated therewith increase.

  On the other hand, deep ocean water refers to seawater collected from the deep sea at a depth of 200 meters or less from the sea surface, but its physicochemical properties are known to be different from surface seawater (for example, non-patent literature). 3). One of the characteristics is mineral, which is characterized by containing a mineral component essential for the human body (for example, see Patent Document 3). It is already known that deep ocean water exerts an effect of promoting absorption promotion of vitamin C, which is a water-soluble vitamin (see, for example, Patent Document 3), but fat-soluble carotenoids (for example, non-patents) Up to now, no absorption-promoting action has been discovered.

JP 2008-297215 A JP 2008-79512 A JP 2010-274214 A

“Carotenoids: Their Diversity and Physiological Activity” (Mamuro Mamoru, Takaichi Shinichi, Tomita Junfumi) Pharmacology and treatment Vol. 36 Examination of the usefulness of the body fat reduction effect by ingestion of β-cryptoxanthin-containing beverages derived from Satsuma mandarin (Takashi Tsuchida et al.) No. 3, 247-253 (2008) “Science of water” (Yoshinori Kawase) Tokyo Denki University Press (2011)

  An object of the present invention is to provide a carotenoid superabsorbent composition that enhances the absorbability of a carotenoid that is hydrophobic to the living body, and that can easily obtain the physiological effect of the carotenoid efficiently by ingesting a small amount.

  As a result of intensive studies, the present inventors have found the effect of promoting carotenoid absorption by combining deep sea water and carotenoids, and have reached the present invention.

That is, the gist of the present invention is the following (1) to (7).
(1) A carotenoid superabsorbent composition containing deep ocean water and carotenoids.
(2) The carotenoid superabsorbent composition according to (1), wherein the carotenoid is derived from citrus.
(3) The carotenoid superabsorbent composition as described in (2) above, wherein the citrus carotenoid is cryptoxanthin.
(4) The deep sea water is fresh water or reverse osmosis membrane method, and is a mixed water obtained by mixing the fresh water and raw deep sea water. (1) to (3) Carotenoid superabsorbent composition.
(5) A carotenoid superabsorbent food additive comprising the carotenoid superabsorbent composition according to any one of (1) to (4).
(6) A carotenoid superabsorbent cosmetic comprising the carotenoid superabsorbent composition according to any one of (1) to (4).
(7) A carotenoid superabsorbent pharmaceutical comprising the carotenoid superabsorbent composition according to any one of (1) to (4).

  According to the present invention, the absorbability of carotenoids into a living body can be remarkably improved, so that the physiological effect of carotenoids can be efficiently obtained with a small amount of ingestion.

  Moreover, since the carotenoid superabsorbent composition of the present invention can be used by blending with various products such as food additives, foods and drinks, cosmetics, pharmaceuticals, and pet foods, various products having a carotenoid superabsorbent effect can be provided. .

The examination result of the presence or absence of the beta-cryptoxanthin absorption promotion effect by deep sea water using human colon cancer-derived cell Caco-2 is shown. The examination result of the presence or absence of the astaxanthin absorption promotion effect | action by the deep sea water using human colon cancer origin cell Caco-2 is shown.

  Hereinafter, the invention will be described in detail.

  The deep sea water in the present invention is seawater having characteristics different from the seawater distributed in the surface layer, which is distributed in the deep sea having a depth of 200 meters or deeper, has cleanliness, and is rich in inorganic nutrient salts. It has low temperature stability. In other words, deep ocean water not only contains the minerals essential for humans and other living organisms in a better balance than land water, but also is not contaminated with persistent organic pollutants (POPS) like land water. It has the characteristics of

  The deep ocean water is generally seawater at a depth of 200 m or less which is not reachable by the sun, and has excellent resource properties such as eutrophication, cleanliness, low water temperature, and water quality stability. From the viewpoint of resource utilization, deep ocean water is defined as “highly resourced seawater deeper than the compensation depth, where organic matter decomposition is superior to organic production by photosynthesis, and there is less vertical mixing and human influence”. In other words, deep ocean water is rich in nutrients rich in inorganic salts such as nitrates and phosphates, cleanliness with less residual organic compounds besides bacteria and microorganisms, low water temperature without temperature rise due to solar radiation, Characterized by water quality stability with small fluctuations throughout the year.

  Above all, the deep sea water obtained in Izu Akazawa has a higher carotenoid absorbability than the deep sea water obtained in other intake areas because it contains abundant inorganic salt nutrients. In particular, among deep sea water of Izu Akazawa, deep sea water pumped from a depth of 800 m off Izu Akazawa has particularly high carotenoid absorption.

  The carotenoid in the present invention is not particularly limited, but α-cryptoxanthin, β-cryptoxanthin, isocryptoxanthin, actinoerythrol, astaxanthin, astaxin, adonixanthin, phenicoxanthine, β-apo-8′-carotenal, β-apo-8′-carotenol, alloxanthin, anteraxanthin, isoagelaxanthin, isoseasatintin, isoreniellaten, β-isorenieraten, idoxanthin, equinone, 3′-epilutein, erythroxanthin sulfate, Okenone, osylaxanthin, capsanthin, cabsorbin, caroxanthin, α-carotene, β-carotene, β-carotene, δ-carotene, ε-carotene, ε, ε-carotene-3.3'-diol, η-carotene, ζ -Carotene, Kanta Santin, guilloxanthine, guaaxanthin, crystalaxanthin, crocoxanthin, crocetin, chloroxanthine, chlorobactene, ketomyxocoxanthin, ketomixol fucoside, geliodesxanthin, zynoxanthine, thermozeaxanthin, diazinoxanthine, diatoxanthine Diaponeurosporene, diapophytoene, 4.4'-diapolycopene dial, 2,2'-diketospiriloxanthine, 3,4-dehydrorhodopine, dehydrolycopene, citranaxanthin, citranaxanthin , Siphonaxanthin, staphyloxanthine, spiroxanthin, spheroidenone, sphenoidene, zeaxanthin, taraxanthin, tunaxanthin, deoxyflexoxanthin, decaprenoxanthin, Thiaten, demethylspheroidene, paplioerythrin, trikentriorhodin, tolralozin, tolylene, neurospoxanthine, neurosporen, neoxanthine, nostoxanthin, bacteriorubixanthin, bacteriovelin, bastaxanthin, paplioerythrinone , Paplioerythrin, parasiloxanetin, halacinthiaxanthine, violaxanthin, fizzarien, phytoene, phytofluene, philipsiaxanthine, phenicoxanthine, fucoxanthin, prasinoxanthine, flavuxanthin, frituraxanthin, plectiniaxanthin, hetero Xanthine, peridinin, helenien, bokelia xanthine, myxoxanthine, mytiloxanthin, mutoxanthine, mutochrome Meso-zeaxanthin, monads xanthine, easy Tsuka xanthine, lycopene, lutein, ruby xanthine, rodoxanthin, Rodopinaru, Rodopinoru, Rodobiburin, Rodopin, and the like Lolo xanthine.

  Although it does not specifically limit as a carotenoid in this invention, The fatty acid ester body of the said carotenoid, a sulfate ester body, a hydroxyl group addition body, a dehydroxylation body, a glycoside, etc. are also contained.

  Although it does not specifically limit as said fatty acid ester body, The fatty acid ester, such as lauric acid (C12), myristic acid (C14), palmitic acid (C16), stearic acid (C18), is mentioned.

  Among the above carotenoids, α-cryptoxanthin, β-cryptoxanthin, α-carotene, β-carotene, lycopene, lutein, zeaxanthin, and astaxanthin are preferable, and β-cryptoxanthin is particularly preferable. Particularly preferred. In the present invention, β-cryptoxanthin refers to esters, hydroxyl adducts, dehydroxylated products, and glycosides of β-cryptoxanthin and its fatty acids, like the carotenoids described above.

  The ratio of carotenoid and deep sea water in the composition for highly absorbing carotenoid in the present invention is such that the mass ratio of carotenoid (in terms of dry matter): deep sea water (in terms of dry matter) is 1: 10 to 1: 2000 is preferable, 1:20 to 1: 1000 is more preferable, 1:50 to 1: 1000 is still more preferable, and 1:50 to 1: 700 is particularly preferable.

Content and application amount of carotenoid superabsorbent composition The application amount of the carotenoid superabsorbent composition of the present invention should be appropriately set according to the type of product used, application, expected effect, application form, etc. That's fine. For example, it is preferable to take it as a carotenoid so that the daily intake per adult is 0.001 to 100 g, more preferably 0.01 to 50 g, and still more preferably 0.01 to 30 g.

Form of use of carotenoid superabsorbent composition The application form of the carotenoid superabsorbent composition of the present invention is not particularly limited. For example, oral, transdermal, enteral, transmucosal, intravenous, transarterial, subcutaneous, Although it can be used in any application form such as intramuscular, from the viewpoint of more effectively exerting the carotenoid high absorption action, oral application, transdermal application, and intravenous application are preferable.

  The carotenoid superabsorbent composition of the present invention can be used in any application form and can exert a carotenoid superabsorbent action, so it is incorporated into various products such as food additives, food and drink, pharmaceuticals, cosmetics, feed, pet food, etc. Can be used.

  Further, the dosage form of the product to which the carotenoid superabsorbent composition of the present invention is blended may be any of solid, semi-solid, liquid, etc., and is appropriately set according to the type and use of the product. The In addition, when using this composition as the raw material added to food-drinks, it may be called the food additive for high carotenoid absorption. The product to which the carotenoid superabsorbent composition of the present invention is blended is water, fats and oils, waxes, hydrocarbons, fatty acids, within a range that does not impair the effects of the present invention, depending on the form and the like. Higher alcohols, esters, plant extracts, water-soluble polymers, surfactants, metal soaps, alcohols, polyhydric alcohols, pH adjusters, antioxidants, UV inhibitors, preservatives, fragrances, powders, You may contain additives, such as a thickener, a pigment | dye, and a chelating agent. In addition, the carotenoid superabsorbent composition of the present invention is blended with other carotenoid superabsorbent compositions within a range that does not impair the effects of the present invention, depending on the form and use thereof. Also good. Examples thereof include sulfur-containing molecules such as vitamin C, squalane, niacin, niacinamide, cysteine, and alliin, carotenoids, placenta extract, sorbitol, chitin, chitosan, various plant extracts, and the like. About these compounding quantities, unless the effect of this invention is impaired, it is not limited.

  When the carotenoid superabsorbent composition of the present invention is used in foods and drinks, it is provided as a food or drink that is adjusted to a desired form in combination with other food materials and additive ingredients and exhibits the desired effect. Examples of such foods and drinks include foods for specified health use, dietary supplements, functional foods, foods for the sick, and the like in addition to general foods and drinks. Although it does not restrict | limit especially as a form of these food / beverage products, Specifically, main dishes, such as breads and noodles; Side dishes, such as cheese, ham, wiener, and fishery products; Fruit juice drinks, carbonated drinks, lactic acid drinks, etc. Beverages; Cookies, cakes, jellies, ice cream, pudding, candy, yogurt and other favorite products; tablets, granules, powders, capsules, soft capsules, nutritional drinks and other supplements. These foods and drinks can be used for the applications described above. In addition, the food for the sick can be used as a meal for patients with diseases whose symptoms are expected to be improved by the carotenoid absorption promoting action.

  When the carotenoid superabsorbent composition of the present invention is used for food or drink, the mixing ratio of the agent to the food or drink varies depending on the form of the food or drink, but is, for example, 0.1 to 20% by mass, preferably Is 0.2 to 20% by mass, more preferably 0.35 to 10% by mass.

  When the carotenoid superabsorbent composition of the present invention is used in cosmetics, it is prepared in a desired form in combination with cosmetically acceptable base materials and additive ingredients, and is provided as a carotenoid absorption promoting cosmetic product. . The form of such cosmetics is not particularly limited, and specific examples include emulsions, creams, lotions, packs, cosmetic liquids, detergents, makeup cosmetics, hair cosmetics, and the like.

  When the carotenoid superabsorbent composition of the present invention is used in cosmetics, the blending ratio of the agent with respect to the cosmetics varies depending on the form of the cosmetics, but is, for example, 0.1 to 20% by mass, preferably 0.8. 2-20 mass%, More preferably, 0.35-10 mass% is mentioned.

  When the carotenoid superabsorbent composition of the present invention is used for a pharmaceutical product, the composition of the present invention is used alone or in combination with other pharmacologically active ingredients, pharmaceutically acceptable base materials, additive ingredients, and the like. Prepared as a pharmaceutical for promoting carotenoid absorption. Although it does not restrict | limit especially as a form of such a pharmaceutical, Specifically, oral administration formulation, such as a tablet, a granule, a powder agent, a capsule, a soft capsule, a syrup, external preparation, an inhalant, a suppository, etc. Transdermal or transmucosal preparations; injections and the like.

  When the carotenoid superabsorbent composition of the present invention is used as a pharmaceutical, the blending ratio of the agent with respect to the pharmaceutical varies depending on the form of the pharmaceutical and the like, but is, for example, 0.003 to 50% by mass, preferably 0.00. 03-30 mass%, More preferably, 0.35-30 mass% is mentioned.

  When the carotenoid superabsorbent composition of the present invention is used in feed or pet food, the carotenoid absorption promoter of the present invention and other feed components are combined to prepare a desired form, Served as pet food. The feed ingredient used in the feed or pet food is not particularly limited, but includes, for example, cereals such as corn, wheat, barley and rye; bran such as bran and rice bran; rice cake such as corn gluten meal and corn jam meal Animal feeds such as skim milk powder, whey, fish meal, and bone powder; yeasts such as beer yeast; calciums such as calcium phosphate and calcium carbonate; vitamins; fats and oils; amino acids;

  When the carotenoid superabsorbent composition of the present invention is used in feed or pet food, the blending ratio of the composition with respect to feed or pet food varies depending on the form of the feed or pet food, but for example, 0. 1-20 mass%, Preferably 0.2-20 mass%, More preferably, 0.35-10 mass% is mentioned.

  Regarding the administration or intake of the composition when the carotenoid superabsorbent composition of the present invention is used as cosmetics, foods / drinks, pharmaceuticals, feeds and pet foods, the dosage form of the agent, the age of the subject of administration, and expected The amount effective for carotenoid absorption may be appropriately set according to the effect to be obtained. For example, the daily dose or intake of adults as carotenoids is usually set to about 0.001 to 100 g, preferably about 0.002 to 50 g, and more preferably about 0.005 to 50 g. By satisfying such administration or intake, carotenoid superabsorption can be effectively achieved in vivo.

  The carotenoid superabsorbent composition of the present invention, the carotenoid superabsorbent food additive, and cosmetics, foods and drinks, pharmaceuticals, feeds and pet foods containing the composition, etc., do not impair the effects of the present invention. Thus, various functional components can be blended as necessary. Although it does not specifically limit as a functional component, For example, the composition which accelerates | stimulates absorption of other carotenoids may be sufficient, vitamin C, collagen, squalane, niacin, niacinamide, hyaluronic acid, placenta extract, sorbitol, chitin, Chitosan and other various plant extracts are also included. About these compounding quantities, unless the effect of this invention is impaired, it is not limited.

  The production method of the composition of the present invention will be described below with examples.

  The carotenoid of the present invention can be obtained by a method of extracting from animals or plants or bacteria using a solvent or the like. Further, the carotenoid extract is preferably concentrated by concentrating to dryness by a conventional method.

  When obtaining carotenoids derived from animals and plants, the animals and plants to be used are not particularly limited, but grains such as brown rice, corn, sweet potatoes, sweet potatoes, sweet potatoes such as sweet potatoes, and processed products thereof, azuki bean, green beans, sweet potatoes, sweet potatoes, broad beans , Daizu, Chickpea, Benibanengen, Ramame, Ryoto, Lentil and other beans, Almond, Asa, Sesame, Cashew nut, Kaya, Ginkgo, Chestnut, Walnut, Coconut, Sesame, Shi, Watermelon, tochi, lotus, rhinoceros, pistachio, sunflower, brazil nut, hazelnut, macadamia nut, pine, peanut and other seeds, artichoke, morning glory, tomorrow, asparagus, udon, funny, hill hijiki, okura , Turnip, pumpkin, mustard, cauliflower, kanpyo, chrysanthemum, cabbage, cucumber, Japanese garlic, Kyosai, Mushrooms, Kinsai, Watercress, Hakui, Kale, Kohlrabi, Garbage, Burdock, Komatsuna, Zhasai, Santo Usagi, Shisogarashi, Shiso, Jukusai, Shungiku, Junsai, Ginger, Shiro Zucchini, crispy, celery, mainspring, taasai, daikon, daisai, takana, bamboo shoot, onion, tuna, chicory, chingensai, boar, vine, tsurumura saki, hot pepper, chili, Winter potato, tomatoes, trevis, tonbutsu, nagasaki haikusai, eggplants, nazuna, nigari, leeks, carrots, garlic, green onions, nozawana, nobori, napa cabbage, pakchoi, basil, parsley, sardine konnyaku, Hayatouri, beet , Peppers, hinona, hiroshimana, wipes, buns, block Lee, Spinach, Horseradish, Makomo, Mizukake, Trefoil, Myouga, Mago, Mameha, Sprouts, Morohaya, Yamagobo, Yuri, Yosai, Yomagi, Peanuts, Rakikyo, Riki, Rhubarb, Vegetables such as lettuce, lotus root, shrimp, wasabi, bracken, acerola, avocado, apricot, strawberry, fig, yokan, plum, unshu mandarin orange, olive, orange, oroblanco, persimmon, pumpkin, karin, kiwifruit, kiwano, kumquat , Guava, gooseberry, gummy, grapefruit, gourmet, cherry, sanbokan, shikuwasha, watermelon, sudachi, plum, daikon, tangor, danzero, cherimoya, durian, summer mandarin, natsume, jujube, pineapple, hascup , Hassle, passion flu Tsu, banana, papaya, hyuga natsu, loquat, grape, blueberry, buntan, white support, ponkan, makiwauri, quince, mango, melon, peach, yamomo, yuzu, raspberry, apple, lemon , Aonori, Amanori, Arame, Iwanori, Enogori, Ogori, Kawari, Constricted, Kombu, Suizenji, Amakusa, Tosakari, Hijiki, Hiigashi, Funori, Matsumo, Mudori , Seaweeds such as mozuku, sea bream, sea bream, sea bream, eel, caviar, kochi, salmon / masal, sea bream, sea bream, fish, fish, scallop, sea bream, Abalone, Itayagai, Oyster, Sazae, Shijimi, Tani, Mushroom, Fist, Top shell, Garage, Ribbon, Clam, Scallop , Shellfish such as sea bream, potato, shellfish such as shrimp and crab, cephalopods such as squid and octopus, sea urchin, jellyfish, jellyfish, sea bream, sea cucumber, sea bream, wild boar, etc. , Pigs, rabbits, cows, horses, whales, deer, pigs, noodles, goats, Aigamo, poultry and other meats, eggs, quail eggs, chicken eggs, peas and other eggs, milk such as milk And processed products.

  Among the above-mentioned animals and plants, those derived from citrus are preferable. Citrus in the present invention can include plants belonging to the citrus family and the like. More specifically, Wenzhou orange, Iyokan, Summer orange, Orange, Cabos, Kawabata, Kishumikan, Kiyomi, Kumquat, Grapefruit, Gecki, Sanpokan, Shikuwasa, Jabara, Sweety, Sudachi, Daidai, Tachibana, Dekopon, Natsudaidai, Hassaku, Navel orange, Valencia orange, Evening white cocoon, Hyuganatsu, Bungtan, Ponkan, Mandarin orange, Yatsushiro, Yuzu, Lime, Lemon, Karatachi (including varieties equivalent or similar to these) and the like. Among them, mandarin orange is desirable because it has a high content of cryptoxanthin.

  When obtaining a carotenoid derived from a microorganism, the microorganism to be used is not particularly limited as long as the effects of the present invention are not impaired. Examples include selected microorganisms. Among these, from the viewpoint of carotenoid production ability, bacteria belonging to the genus Paracoccus, Sphingomonas, Brerevundimonas or Erythrobacter are preferred, and bacteria belonging to the genus Paracoccus are more preferred.

  Among the bacteria belonging to the genus Paracoccus, Paracoccus carotinifaciens, Paracoccus marcusii, Paracoccushaeundaensis and Paracoccus zeaxanthinifaciens are preferable, and Paracoccus carotinifaciens is particularly preferable. Specific examples of bacteria belonging to the genus Paracoccus include Paracoccus carotinifaciens E-396 strain (FERM BP-4283) and Paracoccus genus bacteria A-581-1 strain (FERM BP-4671), and these mutant strains. Are also preferably used in the present invention.

  When extracting from animals and plants and / or microorganisms with a solvent, a polar solvent such as water or an organic solvent can be used. The organic solvent is not particularly limited as long as the effects of the present invention are not impaired, but alcohols such as methanol, ethanol, propanol and butanol, polyhydric alcohols such as ethylene glycol, propylene glycol and glycerin, acetone, methyl ethyl ketone and the like. Ketones, esters such as methyl acetate and ethyl acetate, ethers such as tetrahydrofuran and diethyl ether, halogenated hydrocarbons such as dichloromethane, dichloroethane and chloroform, aliphatic hydrocarbons such as hexane and pentane, and aromatics such as toluene Group hydrocarbons, polyethers such as polyethylene glycol, pyridines and the like. These may be used alone or in combination of two or more.

  In particular, when the composition of the present invention is contained in food, it is preferable to use ethanol or hexane among the above organic solvents from the viewpoint of safety. In order to increase the extraction efficiency, water, enzymes, various surfactants, and the like can be used as long as the effects of the present invention are not impaired.

  In the extraction with a solvent, the extraction can be performed a plurality of times. It is also possible to use a supercritical extraction method.

  The extract thus obtained is preferably made into a concentrate by a concentration operation. Examples of the concentration operation include removal of the solvent by using a vacuum concentration device such as an evaporator or heating. Subsequently, the carotenoids with high purity can be obtained by purifying the concentrate by a known purification means. Specifically, for example, the carotenoid is obtained by purifying the extract several times by silica gel column chromatography, or by removing impurities by alkali treatment or solvent fractionation and then purifying by silica gel column chromatography. Can be obtained.

  The deep ocean water of the present invention is more preferably deep ocean water pumped from the aforementioned Izu Akazawa offshore. The method for producing deep ocean water is described in detail below.

  Deep ocean water obtained from the intake site may be used as it is, as long as it does not impair the effects and safety of the present invention, or it can be used as it is, desalination by the reverse osmosis membrane method, cation, anion exchange membrane, electrodialysis, You may use the processed water which performed sterilization processes, such as filter filtration and boiling.

  Desalination by the reverse osmosis membrane method can be carried out by a conventionally known method. For example, a reverse osmosis membrane method using a reverse osmosis membrane device (RO device) using a hollow fiber membrane module can be employed. In such a hollow fiber membrane module, hollow fiber type selectively permeable membrane elements in which hollow fibers are aligned in substantially the same direction are arranged in a container in the longitudinal direction of the hollow fiber.

  Using such a reverse osmosis membrane device, desalination can be performed by flowing deep ocean water into the hollow fiber and collecting the permeated hollow fiber as fresh water. The method using a reverse osmosis membrane device has an advantage that energy consumption is relatively small and dissolved ions can be almost completely removed.

  The raw water of the deep sea water is preferably 0.01 to 5.0% by volume (0.00035 to 0.175% by mass in terms of dry matter), more preferably 0 to the fresh water for dilution obtained from the deep sea water. 0.1 to 0.9% by volume (0.0035 to 0.0315% by mass in terms of dry matter). The fresh water for dilution obtained from the deep ocean water is a high temperature condition (especially in the case of 800 m (Izu Akazawa Deep Water) in the temperature range where the deep ocean water shows the maximum density of water (about 4 ° C), Since it is water stored at 81 atm), it is preferable to dilution of deep ocean water over fresh water derived from other land.

  The absolute amount of mineral contained in the drinking water produced as described above is diluted. However, the mineral balance is kept the same. For example, the mineral ratio of Izu Akazawa deep sea water pumped from a depth of 800 m off Izu Akazawa is potassium: calcium: magnesium: sodium = 1: 1: 3: 24. This ratio is also maintained by dilution.

  Further, the deep sea water used in the present invention may be a salt (dry matter) from which moisture has been removed. The drying method is not particularly limited as long as the effects of the present invention can be obtained, and examples thereof include hot air drying, freeze drying, and spray drying.

  EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples. The deep ocean water used in the examples was pumped from a depth of 800 m off Izu Akazawa.

<Absorption promotion experiment of β-cryptoxanthin by deep sea water using Caco-2 cells>
Test example 1
(Confirmation of intestinal absorption)
In order to examine the intestinal absorbability of the carotenoid superabsorbent composition, examination was performed using Caco-2, which is a human colon cancer-derived cell. Caco-2 cells were seeded at a concentration of 2.0 × 10 ⁵ cells / well in a 6-well plate (Asahi Techno Glass Co., Ltd.) coated with collagen (Nitta Gelatin) and once every 2 days until confluent. The culture was continued while exchanging the medium (DMEM (manufactured by Wako Pure Chemical Industries) + 10% FBS (manufactured by GIBCO) + 1% NEAA (manufactured by Wako Pure Chemical Industries) + 0.5% PSA (manufactured by Wako Pure Chemical Industries)). On the 0th day after the confluence, the culture was continued for 14 days while changing with the same medium once every two days. During this time, samples containing 0.5% by mass and 2.0% by mass (β-cryptoxanthin (hereinafter referred to as “β- CX ”may be referred to))) or added to a final concentration of 2.0% by mass (astaxanthin) and cultured. After 14 days, Caco-2 cells were replaced with Hanks buffer (HBSS, manufactured by Sigma Aldrich) from 30 minutes before the main culture, and the residual FBS was removed by culturing. After 30 minutes, Caco-2 cells were allowed to act on an HBSS mixed solution containing β-cryptoxanthin (manufactured by Shikoku Yasushi Pharmaceutical) and astaxanthin (manufactured by Wako Pure Chemical Industries). Since an additive was added to the basic composition of the HBSS mixture, the composition is shown below.

(HBSS mixture)
Basic composition:
HBSS, 50 μM Taurocholate Na (manufactured by Tokyo Chemical Industry Co., Ltd.), 0.01 mass% M-10D (manufactured by Mitsubishi Chemical Foods)
Additive:
(Β-cryptoxanthin)
Control (DSW-): β-cryptoxanthin 2 μM or 6 μM
0.5% DSW: β-cryptoxanthin 2 μM or 6 μM + final concentration 0.5 mass% DSW
2.0% DSW: β-cryptoxanthin 2 μM or 6 μM + final concentration 2.0 mass% DSW
(Astaxanthin)
Control (DSW-): Astaxanthin reagent 6 μM
2.0% (DSW +): Astaxanthin reagent 6 μM + final concentration 2.0 mass% DSW

  After the above HBSS mixed solution was allowed to act on the cells for 2 hours, the mixed solution was removed, and the cells were washed twice with 1 mM Naurocholate / PBS and once with ice-cold PBS. After removing cryptoxanthin or astaxanthin, the cells were collected and extracted with hexane three times to collect only β-cryptoxanthin or astaxanthin accumulated inside the cells, concentrated with a centrifugal evaporator, and then used for HPLC samples. .

  The result of β-cryptoxanthin in Test Example 1 is shown in FIG. It was found that the uptake action of β-cryptoxanthin was significantly increased in the cells to which DSW was added, compared to the control containing only 2 μM or 6 μM as β-cryptoxanthin. As a result, it was found that β-cryptoxanthin is highly absorbed by the action of DSW.

  The results of astaxanthin in Test Example 1 are shown in FIG. It was found that the uptake action of astaxanthin was significantly increased in cells to which DSW was added, compared to a control containing only 6 μM of astaxanthin reagent. From this, it was found that astaxanthin is highly absorbed by the action of DSW.

Formulation Examples 1-3
Although the compounding composition example of this invention is shown below, this invention is not limited by the composition.
<Formulation example 1>
・ Composition from deep ocean water (raw water) Deep ocean water ... 95% by mass (3.325% by mass in terms of dry matter)
Wenzhou mandarin orange-derived emulsion (β-CX 0.04%) 5 mass%
<Formulation example 2>
・ Composition using freshwater derived from deep ocean water Freshwater derived from deep ocean water: 60% by mass
Deep sea water: 20% by mass (0.7% by mass as dry matter)
Wenzhou mandarin orange-derived emulsion (β-CX 0.04%) 20% by mass
<Formulation example 3>
・ Composition using salt derived from deep ocean water Salt derived from ocean deep water ・ ・ ・ 5% by mass
Wenzhou mandarin orange-derived enzyme-treated powder (β-CX 0.2% by mass) ... 95% by mass

Formulation Examples 4-11
A composition based on deep ocean water (raw water) described in Formulation Example 1 (hereinafter referred to as Composition 1), and a composition derived from deep sea water (fresh water / raw water) described in Formulation Example 2 (hereinafter referred to as Composition 2) Although the composition example when applied to foods and cosmetics using the composition of the deep sea water salt described in Formulation Example 3 (hereinafter referred to as Composition 3) is shown, the present invention is limited by the composition is not.

<Formulation example 4>
The compounding composition of 100g jelly is shown below.
Composition 1 350 mg
Glucose fructose 15g
Citric acid 100mg
Carrageenan 50mg
Locust bean gum 50mg
Perfume Trace Water Residue

<Formulation example 5>
The composition of a 100 ml beauty drink is shown below.
Composition 2 500 mg
Glucose fructose liquid sugar 500mg
Nicotinamide 20mg
Vitamin B1 nitrate 5mg
Vitamin B2 phosphate 5mg
Vitamin B6 5mg
Anhydrous caffeine 50mg
α-Glucosyl hesperidin 5mg
Water residue

<Formulation example 6>
Examples of cosmetic formulations are shown below.
(Lotion)
Composition 2 2.50 mass%
Trisodium citrate 3.00% by mass
Concentrated glycerin 4.00% by mass
1,3-butylene glycol 5.00% by mass
Xanthan gum 0.05% by mass
Sodium hyaluronate 0.01% by mass
Preservative Appropriate amount Purified water Residual

<Formulation example 7>
Examples of cosmetic formulations are shown below.
(Moisturizing cream)
Composition 2 2.50 mass%
Behenyl alcohol 2.00% by mass
Glyceryl trioctanoate 3.00% by mass
Jojoba oil 1.00% by mass
Olive oil 1.00% by mass
Methyl polysiloxane 0.10% by mass
Natural vitamin E 0.10 mass%
POE (20) sorbitan monostearate 2.50 mass%
POE (80) hydrogenated castor oil 0.50% by mass
L-serine 0.50% by mass
Sodium pyrrolidonecarboxylate 0.10% by mass
Carboxyvinyl polymer 0.05% by mass
Potassium hydroxide 0.01% by mass
Preservative Appropriate amount of purified water Residual

<Formulation example 8>
Examples of cosmetic formulations are shown below.
(Milky lotion)
Composition 2 2.50 mass%
Glyceryl trioctanoate 3.00% by mass
Jojoba oil 1.00% by mass
Olive oil 1.00% by mass
Methyl polysiloxane 0.10% by mass
Natural vitamin E 0.10 mass%
POE (20) sorbitan monooleate 1.50% by mass
Sorbitan monooleate 0.50% by mass
L-serine 0.50% by mass
Sodium pyrrolidonecarboxylate 0.10% by mass
Polyvinylpyrrolidone 0.05% by mass
Preservative Appropriate amount Purified water Residual

<Formulation example 9>
Examples of cosmetic formulations are shown below.
(pack)
Composition 2 2.50 mass%
Hydroxyethoxycellulose 3.00% by mass
Carboxyvinyl polymer 1.00% by mass
Polyoxyethylene oleyl ether (15EO) 1.00% by mass
Ethanol 5.00% by mass
Preservative Appropriate amount Purified water Residual

<Formulation example 10>
Examples of cosmetic formulations are shown below.
(Cosmetics for hair)
Composition 2 0.35 mass%
Hardened castor oil (50EO) 0.70% by mass
1,3-butylene glycol 5.00% by mass
Glycerin 1.00% by mass
Ethanol 25.00% by mass
Purified water residue

<Formulation example 11>
Examples of supplement formulation are shown below.
(Soft capsule)
Composition 3 30 mg
Safflower oil 240mg
Beeswax 30mg
Vitamin C 0.1mg
The above composition is mixed and filled into a food soft capsule made of gelatin or the like.

Claims (7)

  A carotenoid superabsorbent composition comprising deep ocean water and carotenoids.   The carotenoid superabsorbent composition according to claim 1, wherein the carotenoid is derived from citrus.   The carotenoid superabsorbent composition according to claim 2, wherein the citrus carotenoid is cryptoxanthin.   The carotenoid superabsorption according to any one of claims 1 to 3, wherein the deep sea water is a raw water or a mixed water in which the fresh water and the deep sea water are mixed with fresh water or a reverse osmosis membrane method. Composition.   A carotenoid superabsorbent food additive comprising the carotenoid superabsorbent composition according to any one of claims 1 to 4.   A carotenoid superabsorbent cosmetic product comprising the carotenoid superabsorbent composition according to any one of claims 1 to 4. A carotenoid superabsorbent pharmaceutical comprising the carotenoid superabsorbent composition according to any one of claims 1 to 4.

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