JP2016086807A - Koji, foods and drinks using koji as raw material, and production method thereof - Google Patents
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Description
本発明は、麹、麹を原料とした飲食品及びその製造方法に関し、特に、穀物等のでんぷん含有物を焙煎処理した後、これを用いて製麹された麹およびそれを原料とした飲食品とその製造方法に関する。 The present invention relates to koji, foods and drinks made from koji, and methods for producing the same, and in particular, after roasting starch-containing materials such as cereals, koji made using the same and food and drink made from the koji Product and its manufacturing method.
従来より、清酒、焼酎、みりん、食酢及び甘酒等の酒類、食品の原料処理は、穀類の精白、洗米、浸漬及び蒸煮からなる。このような従来の原料処理法により醸造された清酒、焼酎等は、通常の市販商品として親しまれている。しかし、近年製品の多様化が進み、これら清酒や焼酎等に関して原料処理に工夫を加えることで、香味の改善された品質の製品が望まれている。例えば特開2000−152774号公報(特許文献1)及び特開2001−269159号公報(特許文献2)に記載されているように原料を焙煎することにより味や香味を向上させる試みが見受けられる。また、特開2011−177109号(特許文献3)に記載されているように焙煎穀物の抽出液を飲料に混合する試みも行われている。 Conventionally, sake, shochu, mirin, alcoholic beverages such as vinegar and amazake, and raw material processing of foods consist of cereal polishing, washed rice, soaking and steaming. Sake, shochu, etc. brewed by such conventional raw material processing methods are popular as ordinary commercial products. However, diversification of products has progressed in recent years, and quality products with improved flavor are desired by adding ingenuity to the raw material processing for these sake, shochu and the like. For example, as described in Japanese Patent Application Laid-Open No. 2000-152774 (Patent Document 1) and Japanese Patent Application Laid-Open No. 2001-269159 (Patent Document 2), attempts to improve the taste and flavor by roasting the raw materials can be seen. . In addition, as described in JP 2011-177109 A (Patent Document 3), an attempt has been made to mix a roasted grain extract into a beverage.
ところで、清酒製造法については、原料としての米、米麹等に使用制限があり、限られた多様化しか図れなかった。例えば、清酒における主たる香気成分は酵母の発酵産物であり、従来の原料処理工程で香味に特徴のある多様化した酒類製品は得られにくいという一面がある。そのような中、特許文献1及び特許文献2に記載されているように原料を焙煎することにより味や香味を向上させる試みも報告されているが、製麹後に焙煎処理を行うことで麹由来のα‐アミラーゼやグルコアミラーゼといった糖化酵素の失活を招く恐れがある。糖化酵素の失活を伴わない程度に焙煎具合を調整することは難しいだけでなく、十分な焙煎香も製品に残りづらくなる。 By the way, as for the sake production method, there are restrictions on the use of rice, rice bran, etc. as raw materials, and only limited diversification has been achieved. For example, the main aroma component in sake is a fermentation product of yeast, and there is one aspect that diversified liquor products characterized by flavor are difficult to obtain in the conventional raw material treatment process. Under such circumstances, attempts have been reported to improve the taste and flavor by roasting raw materials as described in Patent Document 1 and Patent Document 2, but by performing roasting treatment after koji making There is a risk of inactivation of saccharifying enzymes such as α-amylase and glucoamylase derived from koji. Not only is it difficult to adjust the roasting condition to such an extent that saccharification enzyme is not deactivated, but also sufficient roasting incense is difficult to remain in the product.
本発明は、以上のような課題に鑑みてなされたものであり、麹由来の糖化酵素を熱処理により失活させること無く、焙煎処理した穀物等のでんぷん含有物を用いて製麹された麹及びこの麹を原料とした飲食品、並びにその製造方法を提供することを目的とする。 The present invention has been made in view of the above problems, and koji made using starch-containing materials such as roasted grain without inactivating the koji-derived saccharifying enzyme by heat treatment. And it aims at providing the food-drinks which used this candy as a raw material, and its manufacturing method.
このような目的を達成するため、本発明の麹は、穀物等の原料をα化後乾燥したのちに焙煎処理し、その原料を吸水・膨化する際に麹菌分生子を着床させ製麹することを特徴としている。この方法により、焙煎処理時に生成されるピラジン類化合物などの香気成分を含み香ばしい香りを呈した麹を製造することが可能となる。また、この麹を原料として用いることにより、香ばしい香りを呈した清酒等の醸造物や飲料、食品の製造が可能となる。 In order to achieve such an object, the koji of the present invention is made from a raw material such as cereal and then roasted after drying, and when the raw material is water-absorbed and expanded, the koji mold conidia is placed on the koji making It is characterized by doing. By this method, it is possible to produce a koji that contains a fragrance component such as a pyrazine compound produced during the roasting treatment and exhibits a fragrant scent. Moreover, by using this koji as a raw material, it becomes possible to produce brewed products such as sake, beverages and foods with a fragrant fragrance.
本発明を実施するには、穀類の精白、洗米、浸漬及び蒸きょうにより穀類をα化処理後、60℃〜100℃、好ましくは80℃〜95℃で所定の時間、乾燥させることによりα化米を製造する。その際、α化米の水分が10%以下、好ましくは5%以下、さらに好ましくは3%以下となるように時間を調整するのがよい。
このようにして得られたα化米を、次に100℃〜300℃、好ましくは120℃〜220℃、更に好ましくは150℃〜180℃で任意の時間焙煎せしめ、焙煎α化米を得る。乾燥方法に格別な限定はなく、例えば上記温度の温風を用い、必要あればα化米を移動及び/又は攪拌しながら乾燥してもよいし、あるいは適宜な熱源を用いてそのまま乾燥してもよい。この焙煎処理により、ピラジン類化合物が生成されるので、この焙煎処理された穀物を製麹原料の少なくとも一部に用いることにより、製造された飲食品はピラジン類化合物由来の香ばしい香りを呈することになる。
さらに、この状態で焙煎α化米上の雑菌は除去されており、且つ水分含量が低いことから、高い貯蔵性を有している。また、上記の加熱条件をコントロールすることにより、製品への残存ピラジン類化合物量や色調及び/又は香味を自由にコントロールすることができる。In order to carry out the present invention, after cerealization of grains by whitening, washing, dipping and steaming of cereals, the cereals are dried by drying at 60 ° C to 100 ° C, preferably 80 ° C to 95 ° C for a predetermined time. Manufacture rice. At that time, the time should be adjusted so that the water content of the pregelatinized rice is 10% or less, preferably 5% or less, more preferably 3% or less.
The pregelatinized rice thus obtained is then roasted at 100 ° C. to 300 ° C., preferably 120 ° C. to 220 ° C., more preferably 150 ° C. to 180 ° C. for an arbitrary time. obtain. There is no particular limitation on the drying method. For example, using warm air at the above temperature, if necessary, the pregelatinized rice may be dried while moving and / or stirring, or may be dried as it is using an appropriate heat source. Also good. Since this roasting process produces a pyrazine compound, by using the roasted grain as at least a part of the koji raw material, the manufactured food or drink exhibits a fragrant aroma derived from the pyrazine compound. It will be.
Further, in this state, miscellaneous bacteria on the roasted and pregelatinized rice have been removed, and since the water content is low, it has a high storability. Further, by controlling the above heating conditions, the amount, color tone and / or flavor of the remaining pyrazine compound in the product can be freely controlled.
次いで、焙煎α化米に麹菌分生子を着床させる。分生子の着床は、焙煎α化米を吸水させ分生子を散布し播種する方法と、焙煎α化米の吸水時に分生子を分散させた水を用いる方法とがある。例えば、焙煎α化米の吸水時に分生子を分散させた水を用いる場合は、乾燥α化米重量の20%〜100%、好ましくは60%〜80%、さらに好ましくは40%〜50%の水を用い焙煎α化米に播種する。この際、水に分散させる麹菌種および分生子量をコントロールすることで製麹後に得られる酵素バランスをコントロールすることができる。
さらに、麹菌分生子を播種した焙煎α化米を20℃〜50℃、好ましくは30℃〜35℃で一定時間培養することで焙煎米麹を得ることができる。この際、培養温度、培養時間をコントロールすることにより、製麹後に得られる酵素バランスをコントロールすることができる。
上記のように製麹された焙煎米麹は、これを単独で使用することにより(例えば単発酵)、あるいは、酒類醸造時の原材料として使用することにより(例えば並行複発酵)、ピラジン類化合物を付与された醸造物を得ることができる。Next, the Aspergillus oryzae conidia are implanted in the roasted pregelatinized rice. There are two methods of conidia implantation: a method in which roasted pregelatinized rice is absorbed and the conidia is dispersed and seeded, and a method in which conidia are dispersed when water is absorbed in the roasted pregelatinized rice. For example, when using water in which conidia are dispersed during water absorption of roasted pregelatinized rice, 20% to 100%, preferably 60% to 80%, more preferably 40% to 50% of the weight of dry pregelatinized rice Seed roasted α-rice rice with water. At this time, the enzyme balance obtained after koji making can be controlled by controlling the gonococcal species and the amount of conidia dispersed in water.
Furthermore, roasted rice bran can be obtained by culturing roasted pregelatinized rice seeded with Aspergillus oryzae conidia at 20 ° C. to 50 ° C., preferably 30 ° C. to 35 ° C. for a certain period of time. At this time, the enzyme balance obtained after the koji making can be controlled by controlling the culture temperature and culture time.
The roasted rice bran produced as described above can be used alone (for example, simple fermentation), or as a raw material for brewing alcohol (for example, parallel double fermentation), pyrazine compounds Can be obtained.
本発明の麹の製造方法によれば、α化後乾燥した穀物等を焙煎し原料とすることにより、ピラジン類化合物などの香気成分を含み香ばしい香りを呈した麹の提供が可能となる。麹を焙煎する方法では麹の糖化酵素を失活させる恐れがあるが、本発明による麹は、清酒等の醸造物の製造に特段の制限なく使用でき、醸造物に香味を付与することができる。また、焙煎時の加熱条件等をコントロールすることにより、醸造物の色調や香味を自由にコントロール可能である。さらには、清酒等の醸造物のほか、麹を利用した多様な飲料や食品にピラジン類化合物などの香気成分由来の香ばしい香りを付与することが可能となる。 According to the method for producing koji of the present invention, it is possible to provide koji with a fragrant fragrance containing aroma components such as a pyrazine compound by roasting grains and the like dried after pregelatinization and using them as raw materials. Although the method of roasting koji may inactivate koji saccharifying enzymes, koji according to the present invention can be used without particular limitation in the production of brewed products such as sake, and imparts flavor to the brewed products. it can. In addition, the color and flavor of the brew can be freely controlled by controlling the heating conditions during roasting. Furthermore, in addition to brewed products such as sake, it is possible to impart a fragrant fragrance derived from aroma components such as pyrazine compounds to various beverages and foods using koji.
本発明による麹の製造工程を図1に示した。以下、実施例によって本発明を更に具体的に説明するが、本発明はこれらの実施例に限定されるものではない。例えば、原料のでんぷん含有物としては、米のほか、麦やヒエなどの穀類や、白玉粉のような穀物等の加工品が利用できる。 The manufacturing process of the bag according to the present invention is shown in FIG. EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples. For example, as a raw material starch-containing material, grains, such as wheat and barnyard millet, and processed products such as grains such as white flour can be used in addition to rice.
清酒製造用玄米を精米歩合65%まで精米後、洗米、吸水、蒸きょうし、荒熱を除去した後に95℃の熱風乾燥機にて20時間乾燥させα化米を得た。その後、120℃、150℃、180℃でそれぞれ2時間、4時間、6時間、8時間焙煎し、焙煎α化米を得た。市販麹菌の分生子0.2gを5mLの水に分散させ、焙煎α化米10gに加え、プラスチックシャーレ(φ90×15mm)にて、35℃/48時間で培養し、焙煎米麹を得た。
対照として清酒製造用玄米を精米歩合65%まで精米後、洗米、吸水、蒸きょうし、荒熱を除去した後に95℃の熱風乾燥機にて20時間乾燥させα化米を得た。市販麹菌(株式会社秋田今野社製)の分生子0.2gを5mLの水に分散させ、α化米10gに加え、プラスチックシャーレ(φ90×15mm)にて、35℃/48時間で培養し、α化米麹を得た。
対照として清酒製造用玄米を精米歩合65%まで精米後、洗米、吸水、蒸きょうし、荒熱を除去した後に95℃の熱風乾燥機にて20時間乾燥させα化米を得た。市販麹菌の分生子0.2gを5mLの水に分散させ、α化米10gに加え、プラスチックシャーレ(φ90×15mm)にて、35℃/48時間で培養し、α化米麹を得た。その後、180℃で5分、10分、15分、30分、60分間焙煎し、α化米焙煎麹を得た。Brown rice for refined sake was polished to a polishing rate of 65%, washed with rice, absorbed in water, steamed, removed from rough heat and dried in a hot air dryer at 95 ° C. for 20 hours to obtain pregelatinized rice. Thereafter, roasting was performed at 120 ° C., 150 ° C., and 180 ° C. for 2 hours, 4 hours, 6 hours, and 8 hours, respectively, to obtain roasted α-rice. 0.2 g of conidia of commercially available koji molds are dispersed in 5 mL of water, added to 10 g of roasted α-rice, and cultured at 35 ° C./48 hours in a plastic petri dish (φ90 × 15 mm) to obtain roasted rice bran It was.
As a control, brown rice for sake production was refined to 65% of the polished rice, washed with water, absorbed, steamed, removed from rough heat and dried in a hot air dryer at 95 ° C. for 20 hours to obtain pregelatinized rice. 0.2 g of conidia of commercially available koji mold (manufactured by Akita Imano Co., Ltd.) is dispersed in 5 mL of water, added to 10 g of pregelatinized rice, and cultured in a plastic petri dish (φ90 × 15 mm) at 35 ° C./48 hours, Obtained alpha rice bran.
As a control, brown rice for sake production was refined to 65% of the polished rice, washed with water, absorbed, steamed, removed from rough heat and dried in a hot air dryer at 95 ° C. for 20 hours to obtain pregelatinized rice. 0.2 g of conidia of commercially available koji molds were dispersed in 5 mL of water, added to 10 g of pregelatinized rice, and cultured at 35 ° C./48 hours in a plastic petri dish (φ90 × 15 mm) to obtain pregelatinized rice bran. Then, it roasted at 180 ° C. for 5 minutes, 10 minutes, 15 minutes, 30 minutes, and 60 minutes to obtain pregelatinized rice roasted rice cake.
上記麹の酵素力価をキット(株式会社キッコーマン 糖化力分別定量キットおよびα‐アミラーゼ測定キット)を用いて測定した。その結果を図2に示す。その結果、α化米を焙煎することにより酵素力価は低減するものの、発酵工程で十分な糖化が可能な領域であるグルコアミラーゼ100U/g以上、α‐アミラーゼ500U/g以上を保持することを見出した。また、α化米焙煎麹は5分程度の焙煎工程で元の酵素力価の約27%程度まで失活し、香味もロースト感の薄いものだった。 The enzyme titer of the above koji was measured using kits (Kikkoman Corporation saccharification power fractionation quantification kit and α-amylase measurement kit). The result is shown in FIG. As a result, although the enzyme titer is reduced by roasting the pregelatinized rice, it retains glucoamylase of 100 U / g or more and α-amylase of 500 U / g or more, which are areas where sufficient saccharification is possible in the fermentation process. I found. In addition, the pregelatinized rice roasted rice cake was deactivated to about 27% of the original enzyme titer in the roasting process for about 5 minutes, and the flavor was lightly roasted.
上記のように作成した焙煎米麹に含有されるピラジン類化合物をGC/MS法で測定し、その結果をα化米麹を対照として図3に示した。なお、ここで言うピラジン類化合物とは2−メチルピラジン、2,6−ジメチルピラジン、2,3,5−トリメチルピラジン、2−エチル−3−メチルピラジン、2,5−ジメチルピラジンを指し、濃度はそれぞれの合算値である。
この結果より、焙煎温度および焙煎時間に応じてピラジン類化合物の濃度が増加することが認められた。これは、精白米を焙煎処理することで、精白米が含有する糖類や蛋白質が加熱されてα−ジカルボニル化合物とα−アミノ酸との反応であるアミノカルボニル反応を経て、ピラジン類化合物が生成されたことによるものである。また、選抜されたパネラー3名により官能評価を行った結果、焙煎温度120℃の場合で8時間、150℃の場合で6時間以上、180℃の場合で2時間以上、焙煎することによりロースト感を得られることが明らかとなった。The pyrazine compounds contained in the roasted rice bran prepared as described above were measured by the GC / MS method, and the results are shown in FIG. In addition, the pyrazine compound mentioned here refers to 2-methylpyrazine, 2,6-dimethylpyrazine, 2,3,5-trimethylpyrazine, 2-ethyl-3-methylpyrazine, and 2,5-dimethylpyrazine. Is the sum of each.
From this result, it was recognized that the concentration of the pyrazine compound increased according to the roasting temperature and roasting time. This is due to roasting of milled rice, the saccharides and proteins contained in milled rice are heated, and an aminocarbonyl reaction, which is a reaction between an α-dicarbonyl compound and an α-amino acid, is performed to produce a pyrazine compound. It is because it was done. In addition, as a result of sensory evaluation by three selected panelists, by roasting at a roasting temperature of 120 ° C for 8 hours, at 150 ° C for 6 hours or more, at 180 ° C for 2 hours or more It became clear that a roasted feeling could be obtained.
本発明により、清酒等の醸造物のほか、麹を利用した多様な飲料や食品の産業分野に、ピラジン類化合物などの香気成分由来の香ばしい香りを呈した新たな製品の創出が可能となる。清酒、焼酎、みりん、食酢、味噌等の醸造物のほか、甘酒、菓子など麹を原料とした飲食品や、漬物など農林水産物の加工品も本発明の飲食品である。 According to the present invention, it is possible to create a new product exhibiting a fragrant fragrance derived from an aroma component such as a pyrazine compound in various industrial fields of beverages and foods using koji as well as brewed products such as sake. In addition to brewed products such as sake, shochu, mirin, vinegar, miso, etc., foods and drinks made from koji such as amazake and sweets, and processed products of agricultural, forestry and fishery products such as pickles are also food and drink of the present invention.
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CN116349830A (en) * | 2023-05-05 | 2023-06-30 | 安徽农业大学 | Method for improving production efficiency of red rice by preprocessing long-shaped rice |
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