JP2016044129A - Moisturizing agent and external preparation for skin containing it - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for effective utilization of a pineapple core.SOLUTION: A pineapple core is contacted to a cellulase enzyme agent derived from Trichoderma microorganisms such as Trichoderma reesei and Aspergillus microorganisms such as Aspergillus niger preferably under the condition of 45-50°C and pH 5-7, and an enzyme-treated material thereof, preferably a supernatant thereof is used as a moisturizing agent. Preferably a fruit core subjected to heat treatment or delignification treatment is used upon enzyme treatment.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a moisturizing agent and an external preparation for skin containing the same.

  Pineapple is a typical edible plant that eats juice and fruits. However, the actual situation is that the soft pulp portion is usually eaten, and the core portion having a hard tissue is cut out and disposed of as waste.

  In addition to its use as a dietary fiber (food fiber), the pineapple fruit core is used as a moisturizing function improving agent or a barrier function improving agent by extracting a hydroxy fatty acid derivative from the edible portion containing the fruit core. (Patent Document 1), Extracting glucosylceramide from the edible part containing the fruit core and using it as a raw material for cosmetics excellent in preventing reduction in the amount of moisture in the epidermis caused by UV exposure (Patent Document 2), including the fruit core It is known that fruit water obtained in a step of concentrating fruit juice obtained from fruits is used as a raw material for cosmetics (Patent Document 3).

  In addition, the fruit juice of pineapple and its extract have antioxidant, anti-inflammatory and melanin production inhibitory effects (Patent Document 4), and the prevention of foot-and-mouth disease in pineapple fruit, its juice and extract. Or it is known that there is a therapeutic effect (Patent Document 5).

  However, these methods are methods that mainly use fruit juice including the edible portion, and a method that effectively uses the fruit core portion, which can be said to be a pineapple juice residue, has been desired.

  Further, the composition of the pineapple fruit core has been investigated by Pardo et al. (Non-patent Document 1), but it has been unknown so far whether or not the fruit core can be effectively used by enzyme treatment.

JP 2012-158573 A JP 2013-241370 A Japanese Patent Laid-Open No. 10-287526 JP 2002-275081 A JP 2003-201245 A

Pardo et al., J. Agric. Chemistry and Enviroment, 2014, 3, 53-56

  This invention is made | formed in view of the said background art, Comprising: It is providing the method of using effectively the fruit core of a pineapple.

  The present invention is to use a processed product obtained by subjecting a pineapple fruit core to a cellulase treatment as a humectant.

  A moisturizing agent having a pineapple-specific scent and a skin external preparation using the same are provided.

FIG. 1 is a chart showing analysis results of constituent sugars in a cellulase-treated product of pineapple fruit core. The numbers in FIG. 1 correspond to the enzyme numbers in Table 1. FIG. 2 is a chart showing the effect of the presence or absence of heat treatment. FIG. 3 is a graph showing changes in transepidermal moisture transpiration during application of a pineapple fruit core treated product. FIG. 4 is a graph showing a comparison of the stratum corneum moisture content when a pineapple fruit core processed product is applied.

  The humectant according to the present invention is obtained by subjecting a pineapple fruit core to an enzyme treatment with a cellulase enzyme agent. The pineapple fruit core used in the present invention is a part obtained by removing the edible part from the fruit of a plant belonging to the genus Ananas of the pineapple family, and is a part usually discarded. The present invention uses the discarded portion, and an edible portion may be included.

  As the cellulase enzyme agent, various cellulase enzyme agents prepared from cellulase-producing bacteria and marketed can be used. The origin of the cellulase enzyme is not limited, and examples include cellulase enzymes derived from the genus Aspergillus, such as Aspergillus niger, and enzyme agents derived from Trichoderma reesei. Cellulase is an enzyme that degrades the glycosidic bond of β-1,4-glucan, and the cellulase enzyme agent contains one or more cellulases. In the enzyme treatment, one kind of cellulase enzyme agent is used, but two or more kinds of cellulase enzyme agents may be used.

  The enzyme treatment is performed by bringing a pineapple fruit core into contact with a cellulase enzyme agent. The conditions for enzyme treatment can be appropriately determined by those skilled in the art. Examples of the treatment conditions include a reaction temperature of 30 to 60 ° C., preferably 40 to 55 ° C., more preferably 45 to 50 ° C., and pH 4 to 8, preferably pH 5 to 7, and a contact time of 8 to 24 hours. The enzyme concentration varies depending on the substrate concentration (fruit core amount), but is 0.1 to 5%, preferably 0.5 to 2% when the substrate concentration is 25 to 50%.

  In contact with the cellulase enzyme agent, the pineapple fruit core is preferably cut and / or crushed in advance. Moreover, the heat processing by an autoclave and boiling can be performed as arbitrary processing. The conditions for the heat treatment are not particularly limited, and examples include high pressure steam treatment conditions of 1 to 3 atm, 105 ° C. to 150 ° C. and 5 to 30 minutes, and heating conditions of 30 minutes to 2 hours in boiling water. The

  The enzyme-treated product thus obtained contains oligosaccharides such as arabinose and xylobiose and vascular fibers. In particular, the reaction solution excluding the fiber contains various oligosaccharides and has an excellent moisturizing effect. It also has a pineapple-specific fragrance and functions as a fragrance.

  The enzyme-treated product is separated into a reaction solution and a fiber as a residue thereof by a method such as centrifugation or filtration, and the reaction solution can be blended in a skin external preparation such as a cosmetic as a moisturizing agent. Moreover, when there are few residues etc., the whole reaction material (processed material) may be mix | blended, without isolate | separating both.

  Furthermore, delignification treatment is performed when it is desired to reduce the fiber. The method of delignification treatment is not particularly limited, and for example, an alkaline aqueous solution of about 1 to 10% hypochlorous acid is heated at room temperature or about 30 to 50 ° C. for 30 minutes to several hours, It is performed by immersing the above fiber. The delignification treatment may be performed at any time before or after contact with the cellulase enzyme agent. When it is carried out before the contact with the cellulase enzyme agent, the cellulase enzyme after the delignification treatment is brought into contact with the cellulase enzyme agent or the pH of the delignification treatment liquid containing the fruit core is adjusted and then brought into contact with the cellulase enzyme agent. In addition, when it is performed after contact with the cellulase enzyme treatment agent, preferably, the fiber taken out from the reaction solution after the enzyme treatment is subjected to delignification treatment, and then contacted with the cellulase enzyme agent and separated first. Used by mixing with the reaction solution. Alternatively, delignification treatment may be performed without separating the fiber from the reaction solution after the enzyme treatment, and the cellulase treatment agent may be contacted again after pH adjustment. The cellulase enzyme agent used in this case is the same enzyme agent as the previously used cellulase enzyme agent, and may be a different enzyme agent. In addition, when performing a heat processing and a delignification process, whichever may be performed first.

  The enzyme-treated product of pineapple fruit core may be used as it is as a humectant, and may be further concentrated and used as an extract-type humectant, or may be dried by a method such as freeze-drying and used as an individual humectant. .

  The external preparation for skin according to the present invention is a composition intended for application to human or animal skin. The use is not limited. For example, the external preparation for skin is a pharmaceutical composition, a cosmetic composition, and may be a composition as a so-called quasi-drug. Moreover, it may be a milky lotion, a cream, an ointment, a liquid like a lotion, or a spray regardless of the dosage form.

  The amount to be added to the external preparation for skin varies depending on the intended dosage form, but is generally from 0.0001 to 99.9% in the composition, preferably from 0.001 to 10%, more preferably from 0.01 to 5%. %.

  Skin external preparations are components necessary for formulation such as water, oil and other carriers, surfactants, preservatives, stabilizers, UV absorbers, UV reflectors, anti-inflammatory agents, antiallergic agents, antioxidants. And various pharmacologically active ingredients such as antibiotics. In addition, a humectant other than the enzyme-treated product of pineapple fruit core may be blended.

  EXAMPLES Hereinafter, although this invention is demonstrated based on the following Example, it cannot be overemphasized that this invention is not limited to the following Example.

[Enzyme treatment with cellulase enzyme]
A commercially available frozen pineapple fruit core was thawed, cut, and crushed with water for about 3 minutes with a mixer. The crushed material was filtered with two non-woven bags for household waste stacked and washed with running water to obtain a sample for enzyme treatment.

  Enzymatic degradation was performed with cellulase enzymes for food shown in Table 1. Suspend the sample in 5 mM acetate buffer (pH 5.0) to 10%, add each cellulase enzyme agent to a concentration of 0.1 to 1%, and react at 37 to 60 ° C. for 15 to 24 hours. It was. After the enzyme reaction, the reaction solution was centrifuged with a centrifuge (10,000 rpm, 4 ° C.). As a result, although variations were observed between the cellulase enzyme agents, most of the cells found in the samples were decomposed by the cellulase treatment, and the vascular fibers found in the samples remained as residues. Among the enzyme agents used, the enzyme agents with less fiber and a relatively large amount of monosaccharide or oligosaccharide were C5, C6, C11, and C19 enzyme agents. Moreover, although reaction progressed between 37-60 degreeC, on the reaction conditions at 45-50 degreeC, the production amount of oligosaccharide was large and it was preferable temperature conditions.

  Next, degradation products were examined from the supernatant by size exclusion HPLC. Size exclusion HPLC was performed using a TOSO-8020 HPLC analysis system loaded with an Oligo PW column (TOSO). As a result, it was found that oligosaccharides of monosaccharide, disaccharide, trisaccharide or more were produced (see FIG. 1). In addition, the total sugar amount of the supernatant was measured by the phenol-sulfuric acid method, and the constituent sugar was analyzed using trifluoroacetic acid (TFA). On average, biose was a constituent sugar. In addition, uronic acid was only about 1% of the total sugar, and most of it was considered to be a neutral sugar. Furthermore, since arabinose, xylose, and cellulose increased by hydrolysis with TFA, it was shown that each oligosaccharide was decomposed into monosaccharides (see Table 2). From these facts, it can be said that an enzyme reaction product containing monosaccharides and oligosaccharides such as arabinose and xylobiose can be obtained from the pineapple fruit core. In addition, the 10% aqueous solution of the supernatant had a sweet scent peculiar to pineapple and was not irritating to the skin.

  In addition, when the fruit core that had been autoclaved at 115 ° C. for 30 minutes was similarly enzyme-treated, the production rate of monosaccharides and oligosaccharides was slightly improved as shown in FIG. In this enzyme treatment, a C11 enzyme agent having a small amount of fiber and a large amount of oligosaccharide produced was used.

  Next, the fiber, which is the residue of the enzyme-treated product obtained in Example 1, was immersed in a 10% aqueous sodium hypochlorite solution at room temperature for 8 hours to perform delignification treatment. Subsequently, each cellulase enzyme agent shown in Table 1 (containing 1% enzyme agent in the reaction solution) was subjected to an enzyme treatment for 15 hours in the same manner as in Example 1. As a result, although there was variation between the enzymes, a decrease in fiber was observed. In this example, among the above-mentioned enzymes, the enzymes with relatively good solubilization were C1, C2, C5, C7, C11, C12, C14, C18, and C19.

  In addition, when the sugar analysis of the reactants was performed in the same manner as in Example 1, it was confirmed that their presence was almost in the ratio of mannose (2.2 mol), galactose (1.2 mol), and glucose (1 mol). The fiber was solubilized. Thereby, solubilization of the pineapple fruit core is promoted, and a humectant with less fiber can be provided.

  Next, a lotion having the formulation shown in Table 3 was prepared using the supernatant obtained in Example 1 (treated with an enzyme preparation of C11), and the moisturizing effect was confirmed. The moisturizing effect was confirmed by measuring the transepidermal moisture transpiration (TEWL) of the application part and the moisture content of the stratum corneum.

[Transepidermal moisture transpiration]
Lotion was applied to the inner side of the forearm twice a day for 7 days, and the transepidermal water transpiration amount of the application part was measured before application, 5 days after application, and 10 days after application. Transepidermal water transpiration was measured using a skin water transpiration measuring device TEWAMETER TM210 (COURAGE + KHAZAKA Electronic GmbH). The results are shown in FIG. As a result, the amount of water transpiration from the skin could be suppressed by applying the skin lotion containing the pineapple fruit core enzyme-treated product.

[Corn layer moisture content]
A 50% 1,3-butanediol aqueous solution containing 1% of the above supernatant was applied to the inner side of the forearm, and the stratum corneum water content was measured using a skin stratum corneum water content measuring device SKICON-200EX (manufactured by Yayoi Co., Ltd.). It was measured. The stratum corneum moisture content was measured when 50 minutes had elapsed after application, after the application site had been washed after 50 minutes had elapsed, and after 20 minutes had elapsed since washing. The results are shown in FIG. In addition, in FIG. 4, the relative value with respect to the stratum corneum moisture content before application | coating was shown. As a result, compared with the uncoated case, the moisture retention amount of the stratum corneum was increased by applying the pineapple fruit core treated product. Moreover, compared with the water | moisture retention amount falling after water washing in the uncoated part, the high water | moisture content retention was maintained after water washing in the site | part which applied the pineapple fruit core processed material.

  The effective use of the pineapple fruit core according to the present invention can be measured and contributes to the reduction of industrial waste.

Claims (10)

  A humectant containing as an active ingredient a processed fruit core obtained by subjecting a pineapple fruit core to an enzyme treatment with a cellulase enzyme.   The humectant according to claim 1, wherein the pineapple fruit core subjected to the heat treatment and / or delignification treatment is treated with an enzyme.   The humectant according to claim 1 or 2, further comprising a fruit core treated product obtained by subjecting the residue after the enzyme treatment with the cellulase enzyme agent to delignification treatment and further the enzyme treatment with the cellulase enzyme agent.   The humectant according to claim 2 or 3, wherein the delignification treatment is treatment by contact with alkaline hypochlorous acid.   The humectant according to any one of claims 1 to 4, wherein the cellulase enzyme agent includes at least one of a cellulase enzyme agent derived from the genus Turkishderma or a cellulase enzyme agent derived from the genus Aspergillus.   An external preparation for skin containing a processed fruit core obtained by enzymatically treating a pineapple fruit core with a cellulase enzyme.   The skin external preparation of Claim 6 containing the fruit core processed material obtained by carrying out the enzyme process of the pineapple fruit core which performed the heat processing and / or delignification treatment.   The skin external preparation according to claim 6 or 7, comprising a fruit core treated product obtained by subjecting the residue after the enzyme treatment with the cellulase enzyme agent to delignification treatment and further the enzyme treatment with the cellulase enzyme agent.   The skin external preparation according to claim 7 or 8, wherein the delignification treatment is treatment by contact with alkaline hypochlorous acid.   The skin external preparation according to any one of claims 6 to 9, wherein the cellulase enzyme agent includes at least one of a cellulase enzyme agent derived from the genus Turkishderma or a cellulase enzyme agent derived from the genus Aspergillus.