JP2016031334A5

JP2016031334A5 -

Info

Publication number: JP2016031334A5
Application number: JP2014154847A
Authority: JP
Filing date: 2014-07-30
Publication date: 2017-08-10
Anticipated expiration: 2034-07-30

Description

（１−５）試薬分注機構２０８
試薬分注機構２０８は、試料分注機構２０６と同様の構成を有している。すなわち、試薬分注機構２０８は、水平方向への回動及び上下移動可能に構成されており、試薬吸引位置２０９においてプローブの先端を試薬容器２０１Ａ，２０１Ｂ，２０１Ｃ内の試薬に接液させて所定量の吸引を行い、試薬添加位置２２２において反応容器２０５に吐出する。分注後のプローブは、試料分注機構２０６におけるプローブ洗浄機４０３と同様の構成を有するプローブ洗浄機にて試薬成分の洗浄を行う。すなわち、試薬分注機構２０８のプローブ洗浄機では、送水弁を通して洗浄カップに供給される洗浄水により、洗浄カップ内に挿入されたプローブの外面を洗浄するとともに、プローブによって洗浄カップ内の洗浄水を吸い上げ、廃棄ボトルに移動して吐出することでプローブの洗浄を行う。 (1-5) Reagent dispensing mechanism 208
The reagent dispensing mechanism 208 has the same configuration as the sample dispensing mechanism 206. That is, the reagent dispensing mechanism 208 is configured rotated and vertically movably in the horizontal direction, the reagent containers 201A to the tip of the probe in the reagent aspirating position 209, 201B, by wetted the reagent in 201C Tokoro A fixed amount is aspirated and discharged into the reaction container 205 at the reagent addition position 222. After the dispensing, the reagent components are washed by a probe washing machine having the same configuration as the probe washing machine 403 in the sample dispensing mechanism 206. That is, in the probe washing machine of the reagent dispensing mechanism 208, the washing water supplied to the washing cup through the water supply valve is used to wash the outer surface of the probe inserted into the washing cup, and the probe is used to wash the washing water in the washing cup. The probe is washed by sucking up and moving to a waste bottle and discharging.

（１−８．２）捕捉体添加処理
捕捉体添加処理は、捕捉試薬容器２０１Ｂに収容された捕捉試薬（捕捉抗体試薬や捕捉抗原試薬）を試薬分注機構２０８によって反応容器２０５に分注する処理であり、分析対象成分により、捕捉抗体試薬か捕捉抗原試薬の何れかが選択されて用いられる。 (1-8.2) Capturing body addition process In the capturing body addition process, the capture reagent (capture antibody reagent or capture antigen reagent) accommodated in the capture reagent container 201B is dispensed into the reaction container 205 by the reagent dispensing mechanism 208. Either capture antibody reagent or capture antigen reagent is selected and used depending on the component to be analyzed.

１００自動分析装置
１０７ラック
１０８試料容器
１１３ラック搬送ライン
２０１Ａ標識試薬容器
２０１Ｂ捕捉試薬容器
２０１Ｃ反応固相試薬容器
２０３反応ディスク
２０４反応容器設置位置
２０５反応容器
２０６試料分注機構
２０７試料吸引位置
２０８試薬分注機構
２０９試薬吸引位置
２１０分注チップ
２１１反応液吸引機構
２１２反応液吸引位置
２１３緩衝液吸引位置
２１４洗浄液吸引位置
２１５検出ユニット
２１６反応容器移送機構
２１７分注チップ保管部
２１８チップ結合位置
２１９反応容器保管部
２２０チップ破棄位置
２２１試料吐出位置
２２２試薬添加位置
２２３制御部
２４０検量線
４０２反応固相集合機構 100 automatic analyzer 107 rack 108 sample container 113 rack transport line 201A labeling reagent container 201B capture reagent container 201C reaction solid phase reagent container 203 reaction disk 204 reaction container installation position 205 reaction container 206 sample dispensing mechanism 207 sample suction position 208 reagent part Injection mechanism 209 Reagent suction position 210 Dispensing chip 211 Reaction liquid suction mechanism 212 Reaction liquid suction position 213 Buffer liquid suction position 214 Cleaning liquid suction position 215 Detection unit 216 Reaction container transfer mechanism 217 Dispensing chip storage unit 218 Chip coupling position 219 Reaction container Storage unit 220 Chip discarding position 221 Sample discharge position 222 Reagent addition position 223 Control unit 240 Calibration curve 402 Reaction solid phase assembly mechanism

Claims

A procedure for performing a first capture body addition process of adding a capture antibody reagent or capture antigen reagent containing a capture body to a reaction container containing a sample to be analyzed;
A procedure for performing a first labeled body addition process of adding a labeled antigen reagent or labeled antibody reagent containing a labeled body to the reaction container;
A procedure for performing a first reaction solid phase addition treatment in which a reaction solid phase reagent containing a reaction solid phase is added to the reaction vessel;
A procedure for performing a first reaction solid phase washing treatment for washing the reaction solid phase in order to remove components other than the component that forms a bond including the reaction solid phase;
Removing at least a part of the reaction solid phase from the reaction vessel, and performing a first measurement process for measuring a physical quantity related to the labeled body including the reaction solid phase to form a bond;
A procedure for performing a second labeled body addition treatment on the reaction vessel after the first measurement treatment;
A procedure for performing a second reaction solid phase washing treatment after the second labeling agent addition treatment;
A procedure for performing a second measurement process after the second reaction solid phase washing process;
And a procedure for selectively determining one measurement result from a plurality of measurement result candidate values obtained by the first measurement process based on the result of the second measurement process.

A procedure for performing a first capture body addition process of adding a capture antibody reagent or capture antigen reagent containing a capture body to a reaction container containing a sample to be analyzed;
A procedure for performing a first labeled body addition process of adding a labeled antigen reagent or labeled antibody reagent containing a labeled body to the reaction container;
A procedure for performing a first reaction solid phase addition treatment in which a reaction solid phase reagent containing a reaction solid phase is added to the reaction vessel;
Removing at least a part of the reaction solid phase from the reaction vessel, and performing a first measurement process for measuring a physical quantity related to the labeled body including the reaction solid phase to form a bond;
A procedure for performing a second labeled body addition treatment on the reaction vessel after the first measurement treatment;
A procedure for performing a second measurement process after the second labeled body addition process;
And a procedure for selectively determining one measurement result from a plurality of measurement result candidate values obtained by the first measurement process based on the result of the second measurement process.

The analysis method according to claim 1 or 2,
The labeled antibody reagent to said at second labels addition treatment, the label of an amount of 1 / 2-1 / 1000 of the amount of added label in labeled form addition treatment of the first is added The analysis method characterized by adding.

The analysis method according to claim 1 or 2,
The second measurement process includes:
A first measurement for measuring a physical quantity related to the label immediately after the second label addition process;
And a second measurement for measuring a physical quantity relating to the label when the predetermined time after the second label addition treatment has elapsed,
One measurement result is selectively determined from a plurality of measurement result candidate values obtained by the first measurement process based on a difference between the first measurement result and the second measurement result. Analysis method to do.

The first time when a capture antibody or capture antigen containing a capture body, a labeled antigen or label antibody containing a label, and a first reagent containing a plurality of reaction solid phases are added to a reaction container containing a sample to be analyzed A procedure for performing the addition process of
Second time of adding a second reagent containing a capture antibody or capture antigen containing a capture body, a labeled antigen or labeled antibody containing a label, and a reaction solid phase not contained in the first reagent A procedure for performing the addition process;
Removing at least a part of the reaction solid phase from the reaction vessel, and performing a first measurement process for measuring a physical quantity related to the labeled body including the reaction solid phase to form a bond;
A procedure for performing an addition process of adding a reagent containing a labeled antigen or labeled antibody containing a labeled body to the reaction container after the first measurement process;
A procedure for performing a second reaction solid phase washing treatment after the second labeling agent addition treatment;
A procedure for performing a second measurement process after the second reaction solid phase washing process;
And a procedure for selectively determining one measurement result from a plurality of measurement result candidate values obtained by the first measurement process based on the result of the second measurement process.

A sample container for storing a sample to be analyzed;
A labeled reagent container containing a labeled antigen that forms a specific antigen-antibody bond with a component to be analyzed in the sample or a labeled reagent containing a labeled antibody;
A capture reagent container containing a capture antigen that forms a specific antigen-antibody bond with a component to be analyzed in the sample or a capture reagent containing the capture antibody;
A reaction solid phase reagent container that contains a reaction solid phase reagent including a reaction solid phase that forms a specific antigen-antibody bond with a component to be analyzed in the sample;
A reaction container containing a reaction solid phase for dispensing and reacting the sample and the labeling reagent, the capture reagent, and the solid phase reagent;
A sample dispensing mechanism for dispensing the sample from the sample container to the reaction container;
A reagent dispensing mechanism for dispensing the labeling reagent, the supplementary reagent, and the solid phase reagent from the labeling reagent container , the capture reagent container, and the reaction solid phase reagent container , respectively, to the reaction container;
An analysis mechanism for performing qualitative and quantitative analysis of the component to be analyzed based on a physical quantity relating to a label in the reaction container;
A measurement mechanism for performing a measurement process for measuring a physical quantity related to the labeled body in the reaction container;
First capture body addition process for adding the capture reagent to the reaction container containing the sample, first label addition process for adding a labeling reagent to the reaction container, and a reaction solid phase in the reaction container A first solid phase addition treatment for adding a reagent, a first reaction solid phase washing treatment for washing the reaction solid phase in order to remove components other than the components that have formed the antigen-antibody bond including the reaction solid phase, Taking out at least a part of the reaction solid phase from the reaction vessel, and measuring the physical quantity of the labeled body including the reaction solid phase to form a bond, and the measurement process after the first measurement process A second labeling treatment process to the reaction vessel, a second reaction solid phase washing process after the second labeling substance addition process, and a second measurement process after the second reaction solid phase washing process. To the result of the second measurement process Zui, the automatic analyzer characterized by comprising an analysis processor control system for selectively placing one measurement results from a plurality of measurements candidate value obtained by the first measurement process.