JP2016010393A - Installing stand for device for cell segregation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an installing stand for a device for cell segregation capable of easily attaching each part of a device for cell segregation at a correct position and performing a cell segregation operation safely and uniformly.SOLUTION: An installing stand for a device for cell segregation comprises: right/left side plates; a top plate which is unified with or separate from the right/left side plates and connects between top ends or between top parts of the right/left side plates; a horizontal connection plate which is unified with or separate from the right/left side plates and connects between the right/left side plates below the top plate while keeping a space for housing the cell segregation device; a cleaning bag support tool supporting the cleaning bag and a cell-containing liquid bag support tool supporting the cell-containing liquid bag which are provided on a bottom surface of the top plate; a chamber support tool supporting the chamber and a column support tool supporting the cell segregation column which are provided on a top surface of the horizontal connection plate; and a waste liquid bag support tool supporting the waste liquid bag which is provided on a bottom surface of the top plate or a top surface of the horizontal connection plate.

Description

  The present invention relates to a stand on which a cell separation device can be installed.

  In recent years, with rapid advances in hematology and scientific technology, only the necessary blood fractions are separated from body fluids such as whole blood, bone marrow, umbilical cord blood, and tissue extracts and administered to patients. In addition, a treatment style that suppresses side effects by not administering fractions that are not necessary for treatment is widespread.

As a method for performing the blood fractionation, for example, as described in Patent Document 1, an erythrocyte sedimentation agent is added to the body fluid stored in a bag, and the layer is biased toward nucleated cells and platelets, and the erythrocytes are biased. After the nucleated cells and platelets are introduced into the cell trap, and the nucleated cells and platelets are trapped by the filter material, the cell traps are separated into 0. Only the filtrate that has passed through the filter material of 2 volumes or more and less than 1.0 volume is extruded with gas and introduced into the filter material, and mononuclear cells and platelets captured by the filter material are selectively recovered. How to do is known.
When performing this method, for example, the body fluid storage bag 14, the cell trap 11, and the drain bag 12 are arranged in this order as shown in FIGS. (For example, see paragraph 0037 of Patent Document 1). When using gravity, the positions of the above-described parts are generally determined in the vertical direction.

Examples of the stand include a stand provided with a plurality of hands, each of which can place the bag, the cell trap, and the like at a vertical position of a metal shaft member that has been installed vertically. There is a possibility that the cell trap, drain bag, etc. cannot be fixed at the correct position, and even if the positional relationship of each part can be correctly arranged, the overall position becomes higher with respect to the work table, making it difficult to implement etc. There's a problem.
In particular, if the uppermost bag is fixed to the cell strainer, the flow rate will increase, and the contact time between the body fluid and the cell trap may be short, which may reduce the recovery rate of nucleated cells, or the end of the flow Sometimes air enters the cell trap and the nucleated cell recovery rate may be reduced. On the other hand, when the cell trap or the uppermost bag is fixed at a low position, the liquid passing speed is slow and the processing time is long.
In addition, if the position of the waste bag that can hold the liquid drained from the cell trap, such as a drain bag, is low relative to the cell filter, the flow will stop spontaneously while the user is off the seat. However, air may enter the cell trap and the nucleated cell recovery rate may decrease, and conversely, when the drain bag is fixed to a higher position, the liquid passing speed becomes slower and the processing time becomes longer.

  On the other hand, a holding device in which each part is positioned in advance is also included (FIG. 6 of cited document 2). However, this holding device is in an unstable state in which the filter unit 8 for capturing cells is suspended, and when the liquid is passed through such a filter unit, the filter unit tilts and the cells are separated. There is a possibility that it cannot be done sufficiently.

  However, when a rod-shaped stand is used for mounting the cell separation device, know-how is necessary with a commercially available stand, and if the installation method is bad, the original effect of the cell separation device cannot be exhibited, and the cell However, there are cases where sufficient results cannot be obtained even if the separation is possible, such as an increase in loss.

JP 2009-284860 A JP-T-2002-541930

  In view of the circumstances as described above, an object of the present invention is to provide a cell separation device installation stand that can easily attach each part of the cell separation device to the correct position and can safely perform the cell separation operation. It is to provide.

  The present inventors have intensively studied to solve such problems. As a result, the present inventors have succeeded in producing a stand of an unprecedented form that can stably arrange each part of the cell separation device in a correct position in advance, and have completed the present invention.

That is, the present invention provides the following.
(1) A cleaning bag, a cell-containing liquid bag, a chamber connected to the cleaning bag via a first tube, a chamber connected to the cell-containing liquid bag via a second tube, and a third tube connected to the chamber A cell separation column connected via a cell separation device, and a cell separation device having at least a waste liquid bag connected to the cell separation column via a fourth tube, the left and right side plates; The left and right side plates are connected to each other between the upper and lower ends of the left and right side plates, and a top plate that is integral with or separate from the left and right side plates, and has a space for accommodating the cell separation device below the top plate. A horizontal connecting plate that is integral with or separate from the left and right side plates, a cleaning bag support that supports the cleaning bag, and the cell-containing solution provided on the lower surface of the top plate A cell-containing liquid bag support that supports the cell, a chamber support that supports the chamber, a column support that supports the cell separation column, and a lower surface of the top plate. Alternatively, a stand for device installation for cell separation, comprising: a waste liquid bag support provided on an upper surface of the horizontal connecting plate for supporting the waste liquid bag.
(2) The cell separation device installation stand according to (1), wherein the chamber support, the column support, and the waste bag support are arranged in this order while being spaced apart in the left-right direction.
(3) The stand for device installation for cell separation as described in said (2) in which the said column support part is provided in the approximate center part of the left-right direction of the said horizontal connection board.
(4) The cell separation device installation stand according to any one of (1) to (3), wherein the washing bag support and the cell-containing liquid bag support are provided above the chamber support.
(5) The cell separation device installation stand according to any one of (1) to (4), wherein the horizontal connecting plate is disposed above the lower ends of the left and right side plates.
(6) The cell separation device installation stand according to any one of (1) to (5), wherein the column support is a clip having a rotation stop mechanism that matches a shape of the cell separation column.
(7) The cell separation device installation stand according to (6), wherein the clip is a one-side clip.
(8) The stand for device installation for cell separation as described in said (7) by which the window was provided in the center part of the curved-shaped board | plate material which comprises the said clip.
(9) The cell separation device installation stand according to any one of (1) to (8), wherein the column support has a mechanism for reversing vertically.
(10) The cell separation device installation stand according to any one of (1) to (9), wherein the chamber support grips a side surface of the chamber.
(11) The cell separation device installation stand according to (10), wherein the chamber support includes a mechanism capable of tilting the chamber at a predetermined angle.
(12) The cell separation device installation stand according to any one of (1) to (11), wherein a foot is further provided on a lower surface of the side plate.
(13) The stand for device installation for cell separation according to any one of (1) to (12), wherein the washing bag support and the cell-containing liquid bag support are hooks.
(14) The cell separation device installation stand according to (13), wherein the hook is a flat hook.
(15) The cell separation device installation stand according to any one of (1) to (14), wherein the waste liquid bag support has a mechanism capable of adjusting a mounting height of the waste liquid bag.
(16) The cell separation device installation stand according to (15), wherein the waste liquid bag support is a hook.
(17) The cell separation device is a cell separation device in which the third tube has a branch portion in the third tube, and is connected to the cell recovery bag from the branch portion through the fifth tube. The stand for device installation for cell separation of any one of said (1)-(16) arrange | positioned in the lower surface rather than the said horizontal connection board.
(18) The device installation stand for cell separation according to any one of (1) to (17), wherein the side plate includes a connection portion that can be connected to a side plate of another stand.

By using the stand according to the present invention as described above, the device for cell separation can be installed in an appropriate position in a short time, and also for various situations that occur when performing the cell separation operation. There is an effect that the processing operation can be performed safely and uniformly.
Among them, in the stand according to the present invention, the location where the respective parts such as the washing bag, the cell-containing liquid bag, the chamber, the cell separation column, the waste liquid bag, etc. of the cell separation device are preliminarily clarified in the device. Each part can be easily installed without hesitation. In addition, the position of the washing bag, the cell-containing bag, and the column, where the vertical position tends to be a problem when using gravity, is provided with a washing bag support and a cell-containing bag support on the bottom of the top plate. By providing a column support on the upper surface of the horizontal connecting plate, it can be set at a position where an operation using gravity can be easily performed. Further, regarding the position of the waste liquid bag, the positional relationship with the column is easily adjusted by providing the waste liquid bag support on the lower surface of the top plate or the upper surface of the horizontal connecting plate.
Moreover, since the stunt of the present invention is composed of the top plate, the left and right side plates, and the horizontal connecting plate as described above, it is possible to maintain a stable state even when a cell separation device is installed.

It is the schematic front view (a) and schematic back view (b) which show an example of the stand of this invention. It is the perspective view which looked at the stand 1 shown in FIG. It is the perspective view which looked at the stand 1 shown in FIG. It is the schematic seen from the AA part of the stand 1 shown in FIG. 1 in the downward direction. It is the schematic which shows an example of the stand 1 'of another aspect. It is the schematic which shows an example of the cell separation device 14 with which the stand 1 of this invention is mounted | worn. It is the schematic which shows the state which mounted | wore the stand 1 shown in FIG. 1 with the device 14 for cell separation. FIG. 2 is a schematic view showing a state in which two stands 1 are juxtaposed using a fixture 25 and a schematic view of the fixture 25. It is the schematic which shows the state which has arrange | positioned the two stands 1 at an angle using the fixing tool 25 ', and the schematic diagram of the fixing tool 25'. It is the schematic which shows an example of stand 1 '' in which a column support has an inversion mechanism in the up-and-down direction.

(Cell separation device)
The cell separation device installed in the stand of the present invention is composed of a circuit in which an instrument for separating cells is connected by a tube. Specifically, a washing bag, a cell-containing liquid bag, and the washing bag A chamber connected to the cell-containing solution bag via a second tube, a cell separation column connected to the chamber via a third tube, and the cell separation A device having at least a waste liquid bag connected to the column for use via a fourth tube.

  The washing bag refers to a bag containing a washing solution for washing or priming the cell separation column. Examples of the washing liquid include infusion solutions represented by physiological saline, distilled water, Ringer's solution, liquids containing inorganic salts, sugars, serum, proteins, buffers, plasma, culture media, and the like. In particular, a physiological saline solution or an infusion solution can be preferably used from the viewpoint of safety. The cleaning liquid does not need to be one kind of liquid, and two or more liquids from these groups can be mixed and used. As an example of mixing and using two liquids, a physiological saline solution to which citric acid is added can be cited. The term “priming” as used herein refers to an operation of filling the inside of the cell separation device with the washing liquid, and aims to secure the flow path of the cell-containing liquid by removing air from the cell separation column or the circuit.

  The cell-containing liquid bag refers to a bag containing a liquid containing cells to be separated. Examples of cells include induced pluripotent stem cells (iPS cells), embryonic stem cells (ES cells), mesenchymal stem cells, adipose-derived mesenchymal cells, adipose-derived stromal stem cells, pluripotent adult stem cells, bone marrow stromal cells, Multipotent biological stem cells such as hematopoietic stem cells, T cells, B cells, killer T cells (cytotoxic T cells), NK cells, NKT cells, regulatory T cells and other lymphoid cells, macrophages, single cells Examples include somatic cells such as spheres, dendritic cells, granulocytes, erythrocytes, platelets, neurons, muscle cells, fibroblasts, hepatocytes, cardiomyocytes, or cells that have undergone treatment such as gene introduction or differentiation. . Examples of the cell-containing solution include a culture solution of the cells, body fluid such as blood, bone marrow, umbilical cord blood, menstrual blood, lymph, and tissue extract.

  The chamber is for temporarily storing the liquid contained in the washing bag and the cell-containing liquid contained in the cell-containing liquid bag, and for removing air contained in the liquid. It is an instrument. The chamber is made of a transparent material in order to confirm the inside. The passage inside the chamber may be narrowed near the center so that the washing liquid or the cell-containing liquid is dropped as a droplet.

The cell separation column is a column packed with a cell separation material that can capture desired cells and separate them from other cells and other components in the liquid. The upper and lower sides of the column are provided with ports for supplying and discharging liquid.
The material of the cell separation material may be appropriately determined according to the type of target cells, and is not particularly limited.

  The waste liquid bag is a bag for storing various liquids passed through the cell separation column.

  Commercially available products can be used for the washing bag, the cell-containing liquid bag, and the waste liquid bag. These bags are pre-drilled so that they can be used suspended from a predetermined position on the upper side. Also in the stand of the present invention, a cleaning bag support, a cell-containing solution bag support, and a waste solution bag support, which will be described later, are attached to the hole portions of the bags.

  The washing bag is connected to one end of the chamber through a first tube and the cell-containing solution bag is connected through a second tube.

The other end of the chamber is connected to one surface of the cell separation column via a third tube.
The other side of the cell separation column is connected to a waste solution bag through a fourth tube.

  The cell separation device includes a cell collection bag for collecting the cells captured by the cell separation column. The cell collection bag is provided with a branch portion in the third tube, and is connected to the branch portion via a fifth tube.

  Each tube used in the cell separation device may be provided with an air filter or a clamp in order to control liquid flow at a predetermined position. The third tube that connects the cell separation column and the chamber, the fourth tube that connects the cell separation column and the waste bag, and the fifth tube that connects the cell separation column and the cell collection bag are branched portions. A port may be provided via the port. These ports are used to inject liquid for collecting cells, inject priming liquid, collect solution in the bag or cell-containing liquid, as needed, from each circuit of the cell separation device. It is used to drain the solution after it has been collected or to perform sampling.

(stand)
The stand of the present invention is a stand for installing the cell separation device,
A space for accommodating the cell separation device is formed below the top plate, and a top plate that is integral with or separate from the left and right side plates, connecting the left and right side plates and the upper end portions or the upper portions of the left and right side plates. The horizontal connecting plate that is integral with or separate from the left and right side plates, the cleaning bag support that supports the cleaning bag, and the cell-containing liquid bag that are provided on the lower surface of the top plate. A cell-containing liquid bag support that supports the chamber, a chamber support that supports the chamber, a column support that supports the cell separation column, and a lower surface of the top plate or the A waste liquid bag support provided on an upper surface of the horizontal connecting plate for supporting the waste liquid bag.

  In the stand of the present invention, a frame (frame body) is formed by the left and right side plates and the top plate connecting the upper ends or the upper portions of these side plates, and either the front or the back of the opened stand is used. Moreover, a cell separation device can be installed. In addition, when the stand of the present invention is arranged in, for example, a clean bench, there is a type in which air is circulated from the back to the front in order to prevent invasion of germs from outside in the clean bench. In addition to the front of the frame, the back of the stand is open, so there is an advantage that the air flow in the clean bench is not blocked.

The left and right side plates and the top plate may be integrated with each other, or may be separate bodies made of different members. In the case of being integrated, the connecting portion between the left and right side plates and the top plate may be connected to the top plate and the side plate vertically, or the connecting portion is curved. Also good.
In addition, when the left and right side plates and the top plate are separate, it is only necessary to connect the upper end portions or the upper portions of the left and right side plates with a top plate.

  The thickness and width of the top plate and the side plate are not particularly limited as long as the stand can be carried by hand. Moreover, you may match with the place of the objective to install. For example, when the stand is arranged in the clean bench, it may be adjusted according to the size of the inside of the clean bench. Moreover, there is no limitation in particular about the shape of the said top plate and a side plate, It can take arbitrary shapes, such as a plane, a pipe shape, and a square pillar shape.

Further, a hand hole for a handle or a handle may be provided at a predetermined position of the top plate and / or the side plate. By providing such hand holes and handles, the stand can be easily carried.
There are no particular limitations on the number, shape, and position of the hand holes and handles.

  The lower surface of the side plate is a portion that comes into contact with the base when installed. The stand of the present invention can be stably supported because the stand is supported by the left and right side plates as compared with the bar-shaped stand. Further, in order to stably place the stand even when the base on which the stand is placed is not horizontal, a foot may be provided on the lower surface of the side plate. From the viewpoint of stably supporting the stand, the foot only needs to support the one side plate at two or more points. There are no particular limitations on the number and shape of the legs.

Below the top plate, there is provided a horizontal connection plate that is integral with or separate from the left and right side plates and opens the space for accommodating the cell separation device to connect the left and right side plates.
In the stand of this invention, a cell separation device can be accommodated by attaching each part of the said cell separation device to the space enclosed by the said top plate, a side plate, and a horizontal connection board.
The horizontal connecting plate and the left and right side plates may be integrated with each other, or may be a separate body constituted by different members.
The fixing method when the horizontal connecting plate and the left and right side plates are separated is not particularly limited, for example, by fixing with an adhesive or using a fixing tool such as a screw. . Further, the thickness and width of the horizontal connecting plate are not particularly limited.

  The mounting position of the horizontal connecting plate may be near the lower surface of the side plate, but may be adjusted to a position having a predetermined height from the lower surface.

A cleaning bag support that supports the cleaning bag and a cell-containing solution bag support that supports the cell-containing solution bag are provided on the lower surface of the top plate.
The cleaning bag support may be any device that can suspend a cleaning bag such as a hook. The shape of the hook is not particularly limited. However, when the direction of the bag is rotated, it is difficult to see the amount of liquid in the bag, and there is a risk that the tube of the cell separation device may be kinked. Also, if the bag is tilted obliquely, there may be cases where liquid will remain in the bag but the flow through the column will be terminated. it can.

Further, the cleaning bag support and the cell-containing liquid bag support may be installed so as to be shifted in the front or back direction of the top plate so that the attached cleaning bag and the cell-containing liquid bag do not collide with each other. The numbers of the washing bag support and the cell-containing liquid bag support are not particularly limited. For example, when different liquids are used in the priming and cleaning operations, it is preferable to have two cleaning bag supports.
For example, when installing the cell-containing liquid bag after attaching the washing bag to the stand for the first time by providing the washing bag support on the back side of the top plate and the attachment position of the cell-containing liquid bag support on the front side In addition, these bags can be attached without bringing them into contact with each other. Further, on the lower surface of the top plate, the cleaning bag support may be provided on the front side, and the cell-containing liquid bag support may be provided on the back side.

A chamber support for supporting the chamber and a column support for supporting the cell separation column are provided on the upper surface of the horizontal connecting plate.
The chamber support is a member for supporting the chamber, and may be, for example, one that grips a side surface of the chamber.
According to such a configuration, since there is no member to support on the front side of the chamber, the inside of the chamber can be confirmed from the front side.
If the chamber supporter also supports the back side of the chamber, the chamber can be supported more stably.

  The position of the chamber supported by the chamber support is not particularly limited. For example, if the chamber is supported near the center with respect to the vertical direction, the lower part of the chamber is not supported and exposed. It becomes easy to do. In addition, the cell-containing solution may be further passed through the cleaning solution kept inside the chamber, and these liquids may be mixed in the chamber. In this case, if the lower part of the chamber is not supported, this lower part can be touched with a finger. Mixing can be facilitated by tapping lightly and vibrating the chamber.

  The chamber support may include a mechanism capable of tilting the chamber at a predetermined angle. By projecting the lower part of the chamber in the front direction using this mechanism and tilting the entire chamber, the two tubes extending from the upper part of the chamber can be connected to the washing bag and the cell-containing liquid bag without kinking. .

The column support is an instrument that supports the cell separation column. The support method is not particularly limited. For example, a hole having a shape for fitting the lower surface of the cell separation column may be provided, but a clip having a rotation stop mechanism that matches the shape of the column and By doing so, the column can be fixed on the horizontal connecting plate so as not to rotate left and right.
Since the clip is a one-side clip, the cell separation column can be attached and detached more stably.

  In addition, since a window is provided in the central part of the curved plate material constituting the clip, it is possible to check the state of the inside of the cell separation column, particularly air is used for cell separation. Confirmation not to enter the material can be performed. There is no particular limitation on the shape of the window.

  Furthermore, when it is necessary to wash the red blood cells in the cell separation column, the red blood cell removal efficiency is improved by flowing the cell-containing liquid and then turning the cell separation column upside down and then passing the washing liquid. Therefore, it is preferable that the column support has a mechanism for reversing in the vertical direction. Thereby, the cell separation column can be inverted while being held by the column support. The mechanism for reversing the column support in the vertical direction is not particularly limited. For example, the column support is not fixed to the horizontal connection plate, and the support member is fixed to the horizontal connection plate near the back of the column support. The column support and the support member are connected by a single shaft member, and the column support can be rotated about the axis of the shaft member. In addition, when the column support has a mechanism for reversing in the vertical direction, it is preferable to fix the upper and lower portions of the cell separation column in order to stably rotate the cell separation column up and down.

A waste liquid bag support for supporting the waste liquid bag is provided on the lower surface of the top plate or the upper surface of the horizontal connecting plate.
The waste liquid bag support is an instrument that can suspend a waste liquid bag, such as a hook. In particular, if the hook is a hook, it can be inserted into a hanging hole provided in the upper part of the waste liquid bag, so that the waste liquid bag can be easily attached and detached.

  Examples of the waste bag support provided on the lower surface of the top plate include attaching a rod-like member to the lower surface of the top plate and making the tip of the rod-like member a hook or attaching a hook.

Examples of the waste bag support provided on the upper surface of the horizontal connecting plate include attaching a rod-shaped member having a desired height to the upper surface of the horizontal connecting plate, and attaching a hook to the tip.
In this case, the waste liquid bag attached to the waste liquid bag support is attached to the front side of the rod-shaped member so that the inside can be easily seen, but by further hanging the lower part of the waste liquid bag below the horizontal connecting plate, The waste liquid bag can be attached obliquely in the front direction from the top to the bottom of the waste bag. In this way, it is possible to suppress the degree of change in the liquid level when the liquid level in the waste liquid bag gradually rises during device operation than when hanging vertically, by attaching it obliquely in the front direction. Become.

  Further, the waste liquid bag support may have a mechanism capable of adjusting a mounting height of the waste liquid bag. For example, when the height of the waste liquid bag is kept lower than the lower part of the chamber and the lower part of the cell separation column, if the operator leaves the seat in the middle of the liquid passage, the cell separation column after the completion of the liquid passage. The air may invade the cell separation material filled in the cell, and the separation operation may be impaired. On the other hand, if there is a mechanism that can adjust the mounting height of the waste liquid bag, for example, the liquid level in the chamber support and the liquid level in the waste bag are the same height. By adjusting the height, even if there is no liquid in the washing bag or the cell-containing liquid bag, it is possible to temporarily stop the liquid flow naturally with the liquid remaining in the chamber.

  As the mounting height of the waste liquid bag, for example, a position below the chamber (in order to smoothly pass the liquid from the chamber to the waste liquid bag by gravity), near the upper surface of the liquid level in the chamber And a position where the liquid level in the waste liquid bag is parallel (in order to suppress the column liquid flow rate and prevent air from entering the column). If there is a mechanism capable of adjusting these heights, the cell separation operation can be performed efficiently.

As the adjustment mechanism, for example, when the waste liquid bag support is a rod-shaped member attached to the lower surface of the top plate or the upper surface of the horizontal connecting plate, the waste liquid bag is changed by changing the length of the rod-shaped member. The mounting height can be adjusted.
Further, as another aspect of the adjusting mechanism, a hook is previously provided at a plurality of positions in the length direction of the rod-shaped member, or a hole or the like to which the hook can be attached is provided in advance. The mounting height may be adjusted.

  In the stand of the present invention, the chamber support, the cell separation column support, and the waste bag support are separated in this order with respect to the directions of the left and right side plates (left and right direction, length direction of the top plate). By arranging, each instrument without kinking the plurality of tubes connecting the chamber support, the column support for cell separation, and the waste bag support of the cell separation device. Can be attached to the stand.

  In particular, when the cell separation column support is provided at a substantially central portion in the left-right direction of the horizontal connecting plate, the cell separation device can be efficiently mounted on a stand.

  In addition, the position of the cleaning bag support and the cell-containing liquid bag support on the lower surface of the top plate is provided above the chamber support so that the cleaning bag and the cell-containing liquid bag are transferred to the chamber. The liquid can be smoothly passed without the tubes constituting the circuit entangled and kinked.

When the cell separation device is a cell separation device having a branch portion in the third tube and connected to the cell recovery bag from the branch portion via the fifth tube, the cell recovery bag is You may arrange | position on the lower surface rather than the said horizontal connection board.
Since the waste bag and the cell collection bag are similar in shape and are close to each other in the cell separation device, when the cell separation device is attached to the stand, there are cases where the bags are misplaced.
On the other hand, in the stand of the present invention, the waste liquid bag connected to the cell separation column is suspended from the horizontal connection plate or from the inner side of the top plate, while the cell collection bag is placed on the lower surface of the horizontal connection plate. By disposing, the distance between the waste liquid bag and the cell recovery bag can be surely taken, and thus the cell recovery operation can be easily performed without the mistake of the bags at the time of wearing or operation.

Moreover, the stand of this invention can perform several cell separation operation | work simultaneously by making two or more stands parallel.
As a method of arranging a plurality of stands in parallel, for example, it is possible to provide a connecting portion that can connect a side plate of another stand to the side plate.
By having the said connection part, stands can be fixed stably.

As a connection part provided in the said side plate, what is necessary is just to be able to connect mutual side plates, and there is no limitation in particular.
For example, a hole is provided in the side plate, and a fixing tool that fixes the side plates to each other at the hole portion can be used.
In addition, as a form of connection of the stands, the side plates of the two stands may be brought into contact with each other so as to form a straight line. When two or more stands are used, a certain angle is formed. By setting it as such a connection part, you may arrange | position a some stand in polygonal shape according to the number of the stands to be used.

  Materials for the top plate, side plate and horizontal connecting plate constituting the stand include halogenated polymers such as polyvinyl chloride, polyamide, polyimide, polysulfone, polycarbonate, polyethylene, polypropylene, polyvinyl chloride acrylic copolymer, acrylonitrile, butadiene styrene, polystyrene. , Polymethylpentene, acrylonitrile-butadiene-styrene resin (ABS resin), stainless steel, iron, etc., but high resistance to alcohol disinfection, less rusting, light weight, easy to install on clean benches, etc. Therefore, polyvinyl chloride, ABS resin, and stainless steel are preferable.

(cell)
The cells collected in the cell collection bag, which is the object of the present invention, are not particularly limited. For example, artificial pluripotent stem cells (iPS cells), embryonic stem cells (ES cells), mesenchymal stem cells, adipose-derived mesenchyme Biological stem cells, T cells, B cells, killer T cells (cytotoxic T cells), NK cells, such as cell lines, adipose-derived stromal stem cells, pluripotent adult stem cells, bone marrow stromal cells, hematopoietic stem cells Lymphocyte cells such as NKT cells and regulatory T cells, macrophages, monocytes, dendritic cells, granulocytes, platelets, etc., somatic cells such as neurons, myocytes, fibroblasts, hepatocytes, cardiomyocytes, etc. Or the cell etc. which performed processing, such as gene introduction and differentiation, are illustrated.

  Next, embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, the present invention is not limited to the embodiments shown in the accompanying drawings, and includes all the embodiments that satisfy the requirements described in the claims. It is a waste.

As shown in the front view of FIG. 1 (a), the rear view of FIG. 1 (b) and the perspective view of FIG. 2, the stand 1 of the present invention includes a left and right side plate 2 and an upper end portion of the left and right side plates 2. And a top plate 3 connecting the two.
In FIG. 1A, FIG. 1B, and FIG. 2, the connecting surface between the side plate 2 and the top plate 3 is provided with a curved surface, but the side plate 2 and the top plate 3 are directly connected. Also good. Further, the side plate 2 and the top plate 3 are integrated, but may be separate from each other.
Moreover, although the said side plate 2 is installed in the orthogonal | vertical direction with respect to the top plate 3, if the stand 1 is stable, you may extend the lower part of the side plate 3 to an outer side direction, for example.

Below the top plate 3 is provided a horizontal connecting plate 4 that connects the left and right side plates 2 with a space for accommodating the cell separation device.
Both ends of the horizontal connecting plate 4 in the left-right direction are in contact with the surfaces of the left and right side plates 2.

  The horizontal connecting plate 4 is disposed above the lower end portions of the left and right side plates 2. By adopting such a configuration, the cell separation device can be installed on the stand 1 in a more three-dimensional manner, so that the occurrence of tube kinks or misunderstandings caused by forcibly attaching each part is suppressed. Can do.

A cleaning bag support 5 that supports the cleaning bag and a cell-containing solution bag support 6 that supports the cell-containing solution bag are provided on the lower surface of the top plate 3.
The positions of the washing bag support 5 and the cell-containing liquid bag support 6 are as shown in FIGS. 1 (a) and 1 (b), as seen from the front side of the stand 1, and the washing bag support 5 May be on the right side, or the cell-containing solution bag support 6 may be on the right side.

  In addition, as shown in FIG. 3, the cleaning bag support 5 is provided with the cleaning bag support 5 on the back side and the attachment position of the cell-containing liquid bag support 6 on the front side on the lower surface of the top plate 3. After attaching the washing bag to the stand 1 for the first time, when attaching the cell-containing solution bag, these bags can be attached without contacting each other.

  The washing bag support 5 and the cell-containing liquid bag support 6 are both flat hooks, and the hooks face the front, and the hooks are passed through holes provided in the upper part of the washing bag and the cell-containing liquid bag. So you can support without tilting the bag.

  On the upper surface of the horizontal connecting plate 4, a chamber support 7 that supports a chamber, a column support 8 that supports the cell separation column, and a waste liquid bag support 9 that supports the waste liquid bag are provided.

The chamber support 7 includes a gripping portion 7a that grips the side surface and the back surface of the chamber, and a support rod 7b that can dispose the gripping portion 7a at a predetermined height.
The grip portion 7b is configured so that the lower portion of the chamber can be gripped while being inclined in the front direction.

  In addition, since the cleaning bag support 5 and the cell-containing liquid bag support 6 are provided above the chamber support 7, the liquid can be efficiently passed through the two bags.

The column support 8 is a clip having a rotation stop mechanism that matches the shape of the cell separation column. Specifically, the column support 8 can open and close the plate member 8a in a horizontal direction, a curved plate member 8a that matches the shape of the side surface of the column main body, a bottom portion 8b that has a hole that matches the shape of the bottom portion of the column main body, and the plate member 8a. Thus, it comprises a shaft portion 8c that can be fixed to the bottom portion 8b by pivotally supporting the end portion of the plate member 8a. In this column support 8, the column fixes the side surface and bottom surface of the column main body, so that the column can be stably supported.
In the stand 1, the curved plate 8 a of the column support 8 is opened to the front side, a cell separation column is placed on the bottom 8 b from the front side, and the plate 8 a is attached to the cell separation column main body. The column for cell separation can be attached to the column support 8 by returning to the position where it is brought into contact with the surface.

  In addition, a window 12 is provided at the center of the plate member 8 a so that the inside of the column fixed to the column support 8 can be observed through the window 12. The shape of the window 12 may be other shapes as long as the inside of the column can be observed.

The waste liquid bag support 9 is a rod-like member, and is provided with a protrusion at its tip that can be inserted into a hole on the top surface of the waste liquid bag to support the waste liquid bag.
The waste liquid bag support 9 may have a mechanism capable of adjusting the mounting height of the waste liquid bag. For example, by preparing a plurality of types of waste liquid bag support 9 in which the length of the rod-shaped member of the waste liquid bag support 9 is changed, and replacing the waste liquid bag support 9 according to the required length, The mounting height can be adjusted.

  In the stand 1, the chamber support 7, the column support 8, and the waste bag support 9 are arranged in this order while being separated in the left-right direction.

Here, as shown in the schematic diagram of the upper surface of the horizontal connecting plate 4 in the cross-sectional direction of the AA portion in FIG. 4 in FIG. Provided.
The chamber support 7 and the column support 8 are arranged on the front side of the horizontal connecting plate 4 so that the cell separation device can be easily installed from the front side of the stand 1.
On the other hand, by disposing the waste liquid bag support 9 on the back side of the horizontal connection plate 4, the entire waste liquid bag can be inclined by hanging the lower part of the waste liquid bag to the front side of the horizontal connection plate 4. By tilting the entire waste liquid bag in this way, it is possible to moderate the change of the liquid in the waste liquid bag and maintain the liquid passing speed.

  In addition to the above, the stand 1 has the following configuration.

As shown in FIG. 2, the left and right side plates 2 are provided with holes 10 a that are horizontal to the top plate 3, so that the stand 1 can be easily held when the stand 1 is carried upright. Further, the left and right side plates 2 are also provided with a hole 10b that is horizontal with respect to the length direction of the side plate 2, so that the stand 1 can be easily held when the stand 1 is carried sideways.
Note that the shapes of the holes 10a and 10b may be changed as necessary, and handles may be provided on the surfaces of the side plate 2 and the top plate 3 instead of the holes 10a and 10b.

  Legs 13 are provided on the lower surfaces of the left and right side plates 2 so that the stand 1 can be supported more stably.

  Further, as shown in FIGS. 1 (a), 1 (b) and 2, both end portions of the horizontal connecting plate 4 also have reinforcing surfaces 11 facing the lower surface while being in contact with the surface of the side plate 2, thereby The contact area between the connecting plate 4 and the side plate 2 is increased to ensure the fixing, and the strength of the side plate 2 is also reinforced.

Next, FIG. 5 shows another embodiment of the stand 1 ′.
Regarding the stand 1 ′, the description of the same structure as the stand 1 is omitted.

In the stand 1 ′, a waste liquid bag support 9 ′ for supporting the waste liquid bag is provided on the lower surface of the top plate 3.
In the waste liquid bag support 9 ′, a flat hook is provided at the tip of a bar-like member extending downward from the top plate 3.
The waste liquid bag support 9 'may have a mechanism capable of adjusting the height of the waste liquid bag. For example, by preparing a plurality of types of waste liquid bag support 9 'in which the length of the rod-shaped member of the waste liquid bag support 9' is changed, and replacing the waste liquid bag support 9 'according to the required length. The mounting height of the waste liquid bag can be adjusted.

Furthermore, FIG. 10 shows another embodiment of the stand 1 ″.
The stand 1 '' has a mechanism in which the column support 8 rotates in the vertical direction. Specifically, in the stand 1 ″, the bottom 8b of the column support 8 placed on the surface of the horizontal connection plate 4 is not fixed to the horizontal connection plate 4, and the back surface of the shaft portion 8c of the column support 8 is used. A column support rotating fixture 8d that is detachably fixed to the horizontal connecting plate 4 is provided on the side, and the vertical center of the shaft portion 8c and the column support rotating fixture 8d are separated by another shaft member 8e. It is connected so that it can rotate. Then, the column support rotating fixture 8d is removed from the horizontal connecting plate 4, the shaft portion 8c is rotated by 180 ° around the axis of the shaft member 8e, and then the column support rotating fixture 8d is again moved to the horizontal. By attaching to the connecting plate 4, the column support 8 can be turned upside down.

  As another aspect, a space having such a width that the bottom portion 8b does not contact the horizontal connecting plate 4 even when the shaft portion 8c is rotated is formed between the bottom portion 8b of the column support 8 and the horizontal connecting plate 4. You may provide between. According to such a configuration, the column support 8 can be freely turned upside down without removing the column support rotating fixture 8d from the horizontal connecting plate 4. In addition, by inserting another support member in the space between the bottom portion 8b and the horizontal connecting plate 4, it is possible to prevent the cell separation column from being inclined during the cell separation operation.

  Further, in the stand 1 ″, in addition to the bottom portion 8b, a bottom portion 8b ′ for fixing the upper portion of the inverted cell separation column is provided on the shaft in order to invert the column support 8 more stably. It is provided in the upper part of the part 8c. The bottom portion 8b 'has a hole that matches the shape of the bottom portion of the column main body, like the bottom portion 8b, and is fixed to the upper portion of the shaft portion 8c. In this case, the curved plate 8a of the column support 8 is opened to the front side, a cell separation column is inserted from the front side into the space between the bottom portions 8b and 8b ', and the plate 8a is separated from the cell. The column for cell separation can be attached to the column support 8 by returning to the position where it is brought into contact with the surface of the column main body.

  FIG. 6 shows a cell separation device 14 used in the present invention. The cell separation device 14 has at least a cleaning bag 15, a cell-containing liquid bag 16, a chamber 17, a cell separation column 18, and a waste liquid bag 19, and each part is connected by a tube to form a circuit.

  The washing bag 15 and the cell-containing liquid bag 16 are connected to the upper end of the chamber 17 via a first tube 20a and a second tube 20b, respectively.

The lower end of the chamber 17 is connected to the cell separation column 18 via the third tube 20c.
The cell separation column 18 is connected to the waste liquid bag 19 via the fourth tube 20d on the surface of the cell separation column 18 opposite to the side connected to the third tube 20c.

  In addition, a branch portion 22a can be provided in the middle of the third tube 20c in the downward direction of the cell separation column 18, and the fifth tube 20e can be connected to the cell recovery bag 21 from the branch portion 22a. Cells captured by the cell separation column 18 can be collected in the cell collection bag 21.

  The tubes 20a, 20b, 20c, 20d, and 20e may be provided with an air filter, a clamp, or the like (not shown) in order to allow the liquid to flow satisfactorily.

  A branch portion 22b may be provided in the third tube 20c between the branch portion 22a and the chamber 17, and a port 23a may be connected to the branch portion 22b. This port 23a can be used as a priming port for injecting a priming solution.

  A branch portion 22c may be provided in the fourth tube 20d between the cell separation column 18 and the waste liquid bag 19, and a port 23b may be connected to the branch portion 22c. The port 23b can be used as an injection port for a cell recovery solution for injecting the recovery solution into the cell separation column 18 when cells are recovered.

  A branch portion 22d may be provided in the fifth tube 20e between the branch portion 22a and the cell collection bag 21, and a port 23c may be connected to the branch portion 22d. The port 23c can be used as a cell recovery port for recovering cells inside the cell recovery bag 21.

Further, the port 23b used as the injection port and the port 23c used as the cell recovery port are similar in appearance and close in position, so that an error may occur when attaching the cell separation device 14 to the stand 1. Since this may occur, a port attachment 24 may be provided on the side surface of the horizontal connecting plate 4 in the stand 1 as shown in FIGS.
In addition, as the port attachment 24, you may provide the attachment corresponding to each port in an appropriate position, respectively.

FIG. 7 shows a perspective view of the stand 1 with the cell separation device 14 installed.
The cell separation device 14 is accommodated in a space between the left and right side plates 2 and the top plate 3 of the stand 1. In particular, the positions of the chamber 17, the cell separation column 18 and the waste liquid bag 19 can be easily adjusted to an optimal arrangement for cell separation using gravity, and each part can be easily attached and detached.
The waste liquid bag support 9 is supported by hanging the lower part of the waste liquid bag 19 to the front side of the horizontal connecting plate 4 to change the position of the liquid level of the liquid supplied into the waste liquid bag 19. Compared with the case where 19 is supported vertically, the influence on the liquid flow rate using gravity can be suppressed.
In the stand 1, the cell collection bag 21 is disposed on the lower surface of the horizontal connection plate 4, so that, for example, it is possible to avoid a mistake with the waste liquid bag 19, and to perform the cell collection operation safely. Can do.

  As described above, the stand 1, 1 ′, 1 ″ of the present invention is connected to the washing bag, the cell-containing liquid bag, the washing bag through the first tube, and the second cell-containing liquid bag. A cell connected via a tube, a cell separation column connected to the chamber via a third tube, and a cell separation column having at least a waste liquid bag connected to the cell separation column via a fourth tube If it is a device, regardless of the device manufacturer and the difference in the shape of each part, it can be easily installed in a position suitable for cell separation using gravity, and the cell separation operation can be performed safely and uniformly. be able to.

  In addition, since the stand 1 can easily attach and remove the cell separation device 14 as described above, a plurality of cell separation operations can be performed simultaneously by arranging a plurality of stands 1 in parallel.

  For example, when the side plates 2 of the stand 1 are arranged as shown in FIG. 8A, the two side plates 2 are bonded so that the shape of the hole 10a of the side plate 2 matches, and the holes 10a are exposed. A fixing tool 25 is fitted into the cross section of the side plate 2 and fixed. As shown in FIG. 8B, the fixture 25 is provided with a straight groove 26 having a size corresponding to the thickness of the two side plates 2, and the two pieces bonded together in the hole 10a. The stands 1 can be fixed to each other by fitting the groove 26 into the exposed cross section of the side plate 2.

  As shown in FIG. 9B, when a fixture 25 ′ having two grooves 26 that can fix the side plates 2 at a desired angle is used, as shown in FIG. The stand 1 can be arranged at a desired angle. In this case, it can also be arranged in a polygonal shape by increasing the number of stands 1 to be fixed.

  In FIGS. 8 (a) and 9 (a), description of each support tool other than the side plate 2, the top plate 3, the horizontal connecting plate 4, and the fixtures 25 and 25 'is omitted.

1, 1 ′, 1 ″ stand 2 side plate 3 top plate 4 horizontal connecting plate 5 washing bag support 6 cell-containing liquid bag support 7 chamber support 7a gripping part 7b support rod 8 column support 8a curved plate 8b 8b 'Bottom 8c Shaft 8d Column support Rotating fixture 8e Shaft member 9 Waste bag support 10a, 10b Hole 11 Reinforcing surface 12 Window 13 Leg 14 Cell separation device 15 Washing bag 16 Cell-containing liquid bag 17 Chamber 18 Cell Separation column 19 Waste liquid bag 20a 1st tube, 20b 2nd tube, 20c 3rd tube 20d 4th tube, 20e 5th tube 21 Cell collection bag 22a, 22b, 22c, 22d Branch part 23a, 23b, 23c port 24 port Mounting tool 25, 25 'Fixing tool

Claims (18)

  A cleaning bag, a cell-containing liquid bag, a chamber connected to the cleaning bag via a first tube, a chamber connected to the cell-containing liquid bag via a second tube, and a chamber connected to the chamber via a third tube The cell separation column, and a stand for installing a cell separation device having at least a waste bag connected to the cell separation column via a fourth tube, comprising: left and right side plates; Connecting the upper and lower side plates between the upper and lower side plates, or a top plate that is integral with or separate from the left and right side plates, and connecting the left and right side plates below the top plate with a space for accommodating the cell separation device. A horizontal connecting plate that is integral with or separate from the left and right side plates, a cleaning bag support that supports the cleaning bag, and a cell-containing liquid bag that are provided on the lower surface of the top plate. A cell-containing liquid bag support to be supported, a chamber support for supporting the chamber, a column support for supporting the cell separation column, and a lower surface of the top plate or the horizontal provided on the upper surface of the horizontal connecting plate. A stand for installing a device for cell separation, comprising: a waste liquid bag support provided on an upper surface of a connecting plate for supporting the waste liquid bag.   The cell separation device installation stand according to claim 1, wherein the chamber support, the column support, and the waste bag support are arranged in this order while being spaced apart from each other in the left-right direction.   The cell separation device installation stand according to claim 2, wherein the column support portion is provided at a substantially central portion in the left-right direction of the horizontal connecting plate.   The cell separation device installation stand according to any one of claims 1 to 3, wherein the cleaning bag support and the cell-containing liquid bag support are provided above the chamber support.   The cell separation device installation stand according to any one of claims 1 to 4, wherein the horizontal connection plate is disposed above a lower end portion of the left and right side plates.   The stand for installing a device for cell separation according to any one of claims 1 to 5, wherein the column support is a clip having a rotation stopping mechanism adapted to the shape of the column for cell separation.   The cell separation device installation stand according to claim 6, wherein the clip is a one-side clip.   The stand for installing a device for cell separation according to claim 7, wherein a window is provided at a central portion of the bent plate material constituting the clip.   The stand for device installation for cell separation according to any one of claims 1 to 8, wherein the column support has a mechanism for reversing vertically.   The cell separation device installation stand according to any one of claims 1 to 9, wherein the chamber support grips a side surface of the chamber.   The stand for installing a device for cell separation according to claim 10, wherein the chamber support includes a mechanism capable of tilting the chamber at a predetermined angle.   The stand for device installation for cell separation of any one of Claims 1-11 with which the leg is further provided in the lower surface of the said side plate.   The stand for device installation for cell separation according to any one of claims 1 to 12, wherein the washing bag support and the cell-containing liquid bag support are hooks.   The cell separation device installation stand according to claim 13, wherein the hook is a flat hook.   The stand for device installation for cell separation according to any one of claims 1 to 14, wherein the waste liquid bag support has a mechanism capable of adjusting a mounting height of the waste liquid bag.   The cell separation device installation stand according to claim 15, wherein the waste liquid bag support is a hook.   The cell separation device is a cell separation device having a branch portion in the third tube and connected to a cell recovery bag from the branch portion via a fifth tube, and the cell recovery bag is the horizontal The stand for device installation for cell separation of any one of Claims 1-16 arrange | positioned in a lower surface rather than a connection board.   The device separation stand for cell separation according to any one of claims 1 to 17, wherein the side plate includes a connection portion that can be connected to a side plate of another stand.

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