JP2015532424A5 - - Google Patents

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Publication number
JP2015532424A5
JP2015532424A5 JP2015535062A JP2015535062A JP2015532424A5 JP 2015532424 A5 JP2015532424 A5 JP 2015532424A5 JP 2015535062 A JP2015535062 A JP 2015535062A JP 2015535062 A JP2015535062 A JP 2015535062A JP 2015532424 A5 JP2015532424 A5 JP 2015532424A5
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JP
Japan
Prior art keywords
droplets
droplet
microfluidic
zone
storage
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JP2015535062A
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English (en)
Japanese (ja)
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JP2015532424A (ja
JP6282279B2 (ja
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Priority claimed from FR1259566A external-priority patent/FR2996545B1/fr
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JP2015535062A 2012-10-08 2013-10-08 生物学的材料を含有する溶液を処理および分析するためのマイクロ流体工程ならびにこれに対応するマイクロ流体回路 Active JP6282279B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1259566 2012-10-08
FR1259566A FR2996545B1 (fr) 2012-10-08 2012-10-08 Procede microfluidique de traitement et d'analyse d'une solution contenant un materiel biologique, et circuit microfluidique correspondant.
PCT/EP2013/070966 WO2014056930A1 (fr) 2012-10-08 2013-10-08 Procédé microfluidique de traitement et d'analyse d'une solution contenant un matériel biologique, et circuit microfluidique correspondant

Publications (3)

Publication Number Publication Date
JP2015532424A JP2015532424A (ja) 2015-11-09
JP2015532424A5 true JP2015532424A5 (https=) 2016-11-24
JP6282279B2 JP6282279B2 (ja) 2018-02-21

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JP2015535062A Active JP6282279B2 (ja) 2012-10-08 2013-10-08 生物学的材料を含有する溶液を処理および分析するためのマイクロ流体工程ならびにこれに対応するマイクロ流体回路

Country Status (5)

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US (5) US9816133B2 (https=)
EP (1) EP2903738B1 (https=)
JP (1) JP6282279B2 (https=)
FR (1) FR2996545B1 (https=)
WO (1) WO2014056930A1 (https=)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA3216609C (en) 2012-08-14 2024-05-14 10X Genomics, Inc. Microcapsule compositions and methods
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
FR2996545B1 (fr) 2012-10-08 2016-03-25 Ecole Polytech Procede microfluidique de traitement et d'analyse d'une solution contenant un materiel biologique, et circuit microfluidique correspondant.
CN105008895B (zh) 2012-10-15 2019-02-15 纳诺赛莱克特生物医药股份有限公司 颗粒分选的系统、设备和方法
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016904A (es) 2014-06-26 2017-03-27 10X Genomics Inc Analisis de secuencias de acidos nucleicos.
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
DE102014224664B3 (de) * 2014-12-02 2015-10-08 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Vorrichtung und verfahren zur tropfenerzeugung
EP3708256B1 (en) 2015-04-22 2022-11-30 Stilla Technologies Contact-less priming method for loading a solution in a microfluidic device and associated system
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2018099420A1 (zh) * 2016-11-30 2018-06-07 领航基因科技（杭州）有限公司 微滴式数字pcr芯片
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018156935A1 (en) 2017-02-24 2018-08-30 The Regents Of The University Of California Particle-drop structures and methods for making and using the same
US11213824B2 (en) 2017-03-29 2022-01-04 The Research Foundation For The State University Of New York Microfluidic device and methods
CN110945139B (zh) 2017-05-18 2023-09-05 10X基因组学有限公司 用于分选液滴和珠的方法和系统
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US20190064173A1 (en) 2017-08-22 2019-02-28 10X Genomics, Inc. Methods of producing droplets including a particle and an analyte
US11634757B2 (en) 2017-10-20 2023-04-25 Stilla Technologies Emulsions with improved stability
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
JP2019117118A (ja) * 2017-12-27 2019-07-18 株式会社エンプラス 流体取扱方法およびこれに用いる流体取扱装置、ならびに流体取扱システム
WO2019204279A1 (en) 2018-04-16 2019-10-24 Klaris Corporation Methods and apparatus for forming 2-dimensional drop arrays
US11130120B2 (en) 2018-10-01 2021-09-28 Lifeng XIAO Micro-pipette tip for forming micro-droplets
US10486155B1 (en) 2018-10-22 2019-11-26 Klaris Corporation Vacuum-loaded, droplet-generating microfluidic chips and related methods
CN116440969A (zh) 2018-11-27 2023-07-18 斯蒂拉科技公司 具有优化的相流的微流控芯片架构
WO2020109388A1 (en) 2018-11-27 2020-06-04 Stilla Technologies Wells for optimized sample loading in microfluidic chips
WO2020176882A1 (en) 2019-02-28 2020-09-03 10X Genomics, Inc. Devices, systems, and methods for increasing droplet formation efficiency
US12186751B2 (en) 2019-06-28 2025-01-07 10X Genomics, Inc. Devices and systems incorporating acoustic ordering and methods of use thereof
US12059679B2 (en) 2019-11-19 2024-08-13 10X Genomics, Inc. Methods and devices for sorting droplets and particles
US11060141B1 (en) 2019-12-23 2021-07-13 Stilla Technologies Multiplex drop-off digital polymerase chain reaction methods
US10953404B1 (en) 2020-04-24 2021-03-23 Pattern Bioscience, Inc. Apparatuses for contactless loading and imaging of microfluidic chips and related methods
WO2022192683A1 (en) * 2021-03-11 2022-09-15 Virginia Tech Intellectual Properties, Inc. Methods and apparatus for evaporation based liquid transport
CA3215070A1 (en) 2021-04-14 2022-10-20 Joseph DE RUTTE Nanoscale reaction chambers and methods of using the same
CA3155441A1 (en) * 2021-04-20 2022-10-20 National Research Council Microfluidic chip, kit, and system for displacing independent reaction volumes of an emulsion
CN114471765B (zh) * 2022-01-18 2023-04-21 北京保利微芯科技有限公司 离心式液滴生成芯片
CN118201982A (zh) 2022-08-18 2024-06-14 10X基因组学有限公司 具有含氟二醇添加剂的液滴形成装置和方法
EP4521118A1 (en) 2023-09-06 2025-03-12 Stilla Technologies Loading unit for an analysis device

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2720943B1 (fr) 1994-06-09 1996-08-23 Applic Transferts Technolo Emulsions inverses stables à forte concentration en composé(s) fluoré(s) et leur utilisation pour l'administration pulmonaire de médicaments et pour la fabrication d'émulsions multiples.
JP3012608B1 (ja) 1998-09-17 2000-02-28 農林水産省食品総合研究所長 マイクロチャネル装置及び同装置を用いたエマルションの製造方法
JP2001145486A (ja) 1999-11-19 2001-05-29 Natl Inst Of Advanced Industrial Science & Technology Meti 多試料用の微小容量化学反応装置
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
JP2003153692A (ja) 2001-09-07 2003-05-27 Shinji Katsura 核酸増幅方法
EP2302389B1 (en) 2002-05-09 2018-01-24 The University of Chicago Device and method for pressure-driven plug transport and reaction
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20050063875A1 (en) * 2003-09-22 2005-03-24 Georgia Tech Research Corporation Micro-fluidic processor
FR2873171B1 (fr) 2004-07-19 2007-12-07 Centre Nat Rech Scient Cnrse Circuit microfluidique a composant actif
US7759111B2 (en) 2004-08-27 2010-07-20 The Regents Of The University Of California Cell encapsulation microfluidic device
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
ITRM20050389A1 (it) 2005-07-22 2007-01-23 Giuliani Spa Composti e loro sali specifici per i recettori ppar ed i recettori per l'egf e loro uso in campo medico.
DE102005037401B4 (de) 2005-08-08 2007-09-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Bildung einer Emulsion in einem fluidischen Mikrosystem
CA2653321A1 (en) * 2006-05-26 2007-12-06 Althea Technologies, Inc. Biochemical analysis of partitioned cells
FR2901717A1 (fr) 2006-05-30 2007-12-07 Centre Nat Rech Scient Procede de traitement de gouttes dans un circuit microfluidique.
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US20090053719A1 (en) 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
WO2009134395A2 (en) * 2008-04-28 2009-11-05 President And Fellows Of Harvard College Microfluidic device for storage and well-defined arrangement of droplets
CA2738578C (en) 2008-09-23 2021-05-04 Quantalife, Inc. Droplet-based assay system
US8877512B2 (en) 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
FR2950544B1 (fr) 2009-09-29 2011-12-09 Ecole Polytech Circuit microfluidique
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
FR2958186A1 (fr) 2010-03-30 2011-10-07 Ecole Polytech Dispositif de formation de gouttes dans un circuit microfluide.
US9387236B2 (en) 2011-06-10 2016-07-12 Prothera Inc. Pharmaceutical compositions containing protease and methods for the treatment of lysosomal storage diseases
FR2996545B1 (fr) 2012-10-08 2016-03-25 Ecole Polytech Procede microfluidique de traitement et d'analyse d'une solution contenant un materiel biologique, et circuit microfluidique correspondant.

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