JP2015530074A - 生物学的マイクロ物体の結合 - Google Patents

生物学的マイクロ物体の結合 Download PDF

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Publication number
JP2015530074A
JP2015530074A JP2015521839A JP2015521839A JP2015530074A JP 2015530074 A JP2015530074 A JP 2015530074A JP 2015521839 A JP2015521839 A JP 2015521839A JP 2015521839 A JP2015521839 A JP 2015521839A JP 2015530074 A JP2015530074 A JP 2015530074A
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micro
objects
channel
biological
micro object
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JP2015521839A
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English (en)
Japanese (ja)
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JP2015530074A5 (https=
Inventor
ウー，ミン，シー．
チャップマン，ケヴィン，ティー．
カンドロス，イゴール，ワイ．
マチュー，ガエタン，エル．
ショート，スティーブン，ダブリュー．
Original Assignee
バークレー ライツ，インコーポレイテッド
バークレー ライツ，インコーポレイテッド
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Publication of JP2015530074A publication Critical patent/JP2015530074A/ja
Publication of JP2015530074A5 publication Critical patent/JP2015530074A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Fluid Mechanics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2015521839A 2012-07-13 2013-07-12 生物学的マイクロ物体の結合 Pending JP2015530074A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261671499P 2012-07-13 2012-07-13
US61/671,499 2012-07-13
US201261720956P 2012-10-31 2012-10-31
US61/720,956 2012-10-31
PCT/US2013/050269 WO2014011985A1 (en) 2012-07-13 2013-07-12 Combining biological micro-objects

Publications (2)

Publication Number Publication Date
JP2015530074A true JP2015530074A (ja) 2015-10-15
JP2015530074A5 JP2015530074A5 (https=) 2016-09-01

Family

ID=49914304

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015521839A Pending JP2015530074A (ja) 2012-07-13 2013-07-12 生物学的マイクロ物体の結合

Country Status (4)

Country Link
US (1) US20140017791A1 (https=)
EP (1) EP2872615A4 (https=)
JP (1) JP2015530074A (https=)
WO (1) WO2014011985A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016252995B2 (en) * 2015-04-22 2020-12-03 Berkeley Lights, Inc. Freezing and archiving cells on a microfluidic device
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
CN109922885B (zh) 2016-03-16 2022-05-10 伯克利之光生命科技公司 用于基因组编辑克隆的选择和传代的方法、系统和装置
CN110268414B (zh) * 2016-12-01 2023-08-01 伯克利之光生命科技公司 微流体装置中的微物体的自动检测和重新定位
CN110268108B (zh) 2016-12-12 2022-09-06 埃克切拉生物科学公司 用于使用微毛细管阵列进行筛选的方法和系统
EP3562796B1 (en) 2016-12-30 2023-04-19 Xcella Biosciences, Inc. Multi-stage sample recovery system
CA3048645A1 (en) 2016-12-30 2018-07-05 The Regents Of The University Of California Methods for selection and generation of genome edited t cells
KR20200026878A (ko) 2017-06-06 2020-03-11 지머젠 인코포레이티드 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼
SG11202011180UA (en) 2018-05-31 2020-12-30 Berkeley Lights Inc Automated detection and characterization of micro-objects in microfluidic devices
BR112020024839A2 (pt) 2018-06-06 2021-05-18 Zymergen Inc. manipulação de genes envolvidos em transdução de sinal para controlar a morfologia fúngica durante a fermentação e produção
ES3039910T3 (en) 2018-12-06 2025-10-27 Xcella Biosciences Inc Lateral loading of microcapillary arrays
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007537729A (ja) * 2004-04-12 2007-12-27 ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 微粒子及び細胞の操作用の光電子ピンセット

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7901939B2 (en) * 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
US6958132B2 (en) * 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
WO2004103565A2 (de) * 2003-05-19 2004-12-02 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Vorrichtung und verfahren zur strukturierung von flüssigkeiten und zum zudosieren von reaktionsflüssigkeiten zu in separationsmedium eingebetteten flüssigkeitskompartimenten
US20100273681A1 (en) * 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007537729A (ja) * 2004-04-12 2007-12-27 ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 微粒子及び細胞の操作用の光電子ピンセット

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KIRSCHBAUM, M., ET AL.: "Highly controlled electrofusion of individually selected cells in dielectrophoretic field cages", LAB ON A CHIP, vol. 12, no. 3, JPN6017017275, 2012, pages 443 - 450, ISSN: 0003557811 *
NING HU, N., ET AL.: "A high-throughput dielectrophoresis-based cell electrofusion microfluidic device", ELECTROPHORESIS, vol. 32, no. 18, JPN6017017273, 2011, pages 2488 - 2495, ISSN: 0003557810 *

Also Published As

Publication number Publication date
EP2872615A1 (en) 2015-05-20
WO2014011985A1 (en) 2014-01-16
EP2872615A4 (en) 2016-03-30
US20140017791A1 (en) 2014-01-16

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