JP2015509182A - 質量分析法とスコア正規化による微生物の特定方法 - Google Patents
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Abstract
Description
Claims (16)
- 質量分析法によって1組の所定の参照微生物から微生物を特定する方法であって、各参照微生物は1組の参照データにより表され、
特定すべき前記微生物を代表する1組のデータを前記微生物の質量分析法測定に従って決定するステップと、
参照微生物ごとに、決定した前記1組のデータと前記参照微生物の前記1組の参照データとの間の距離を計算するステップと、
前記参照微生物として特定すべき前記微生物に対する確率を、関係
を含む、方法。 - スカラpは全ての参照微生物に対して同一である、請求項1乃至5の何れか1項に記載の方法。
- スカラpは1/Nに等しく、Nは前記1組の参照微生物のサイズである、請求項6に記載の方法。
- スカラpは0.5に等しい、請求項1乃至6の何れか1項に記載の方法。
- 質量スペクトルの決定と、取得したスペクトルと各参照微生物の間の距離の計算によりベクトル分類アルゴリズムを実現する、請求項1乃至8の何れか1項に記載の方法。
- 前記微生物の前記質量スペクトルの決定は、
前記微生物の少なくとも1つの質量スペクトルを取得するステップと、
取得した前記少なくとも1つの質量スペクトルにおけるピークを検出して、検出した前記ピークを所定のベクトル空間のベクトルに変換するステップと、
を含み、
前記微生物と各参照微生物の間の前記距離の計算は、決定した前記ベクトルと、前記参照微生物の第1の部分空間特性と他の参照微生物の第2の部分空間特性の間の前記ベクトル空間を分割する境界と、の間の代数距離を計算するステップを含む、
請求項9に記載の方法。 - 参照微生物の境界は、「サポート・ベクトル・マシン」タイプのアルゴリズムと前記参照微生物に対応する1組のベクトルとによって計算される、請求項9または10に記載の方法。
- 前記ベクトルは、前記質量スペクトルの質量対電荷比の範囲における各所定の再分割区間において高々1つのピークを特定することによって計算される、請求項9、10、または11の何れか1項に記載の方法。
- 質量スペクトルの決定と、取得したスペクトルと各参照微生物の間の距離の計算により許容距離アルゴリズムを実現する、請求項1乃至12の何れか1項に記載の方法。
- 確率f(m)の各々を所定の閾値と比較するステップと、
確率f(m)の全てが前記閾値より小さい場合には、特定すべき前記微生物が前記参照微生物の何れとも対応しないと判定するステップと、
を含む、請求項1乃至13の何れか1項に記載の方法。 - 前記閾値は60%に等しい、請求項14に記載の方法。
- 特定すべき微生物の質量スペクトルを生成可能な質量分光計と、
請求項1乃至15の何れか1項の方法を実施することによって、前記質量分光計により生成された前記質量スペクトルに関連付けられた前記微生物を特定可能な計算ユニットと、
を備える、質量分析法により微生物を特定するための装置。
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US201161566029P | 2011-12-02 | 2011-12-02 | |
EP11306609.6 | 2011-12-02 | ||
US61/566,029 | 2011-12-02 | ||
EP11306609.6A EP2600284A1 (fr) | 2011-12-02 | 2011-12-02 | Procédé d'identification de microorganismes par spectrométrie de masse et normalisation de scores |
PCT/IB2012/056859 WO2013080169A1 (fr) | 2011-12-02 | 2012-11-30 | Procede d'identification de microorganismes par spectrometrie de masse et normalisation de scores |
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JP2015509182A true JP2015509182A (ja) | 2015-03-26 |
JP6027132B2 JP6027132B2 (ja) | 2016-11-16 |
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US (2) | US10546735B2 (ja) |
EP (2) | EP2600284A1 (ja) |
JP (1) | JP6027132B2 (ja) |
CN (1) | CN104040561B (ja) |
ES (1) | ES2665551T3 (ja) |
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WO2020230397A1 (ja) * | 2019-05-10 | 2020-11-19 | 株式会社島津製作所 | 理論質量の外れ値検出方法 |
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FR3009387B1 (fr) * | 2013-07-31 | 2016-11-18 | Biomerieux Sa | Procede et dispositif d'analyse d'un echantillon biologique |
FR3035410B1 (fr) * | 2015-04-24 | 2021-10-01 | Biomerieux Sa | Procede d'identification par spectrometrie de masse d'un sous-groupe de microorganisme inconnu parmi un ensemble de sous-groupes de reference |
CN105447527A (zh) * | 2015-12-31 | 2016-03-30 | 四川木牛流马智能科技有限公司 | 采用图像识别技术将环境微生物进行分类的方法和系统 |
CN105608472A (zh) * | 2015-12-31 | 2016-05-25 | 四川木牛流马智能科技有限公司 | 一种将环境微生物进行全自动分类的方法和系统 |
US10930371B2 (en) * | 2017-07-10 | 2021-02-23 | Chang Gung Memorial Hospital, Linkou | Method of creating characteristic peak profiles of mass spectra and identification model for analyzing and identifying microorganizm |
CN107481240B (zh) * | 2017-08-17 | 2020-06-09 | 重庆青信科技有限公司 | 基于能谱ct图像全分割方法及系统 |
CN107481242B (zh) * | 2017-08-17 | 2020-06-05 | 重庆青信科技有限公司 | 一种能谱ct图像的分割方法及系统 |
WO2019079492A1 (en) * | 2017-10-18 | 2019-04-25 | The Regents Of The University Of California | SOURCE IDENTIFICATION FOR MOLECULES UNKNOWN BY MASS SPECTRUM CORRESPONDENCE |
KR20190074890A (ko) * | 2017-12-20 | 2019-06-28 | 에스케이하이닉스 주식회사 | 메모리 컨트롤러 및 그 동작 방법 |
CN116324418A (zh) * | 2020-10-06 | 2023-06-23 | 赛默飞世尔科学公司 | 用于快速微生物鉴定的系统和方法 |
CN112884663B (zh) * | 2021-01-18 | 2023-11-21 | 北京晶科瑞医学检验实验室有限公司 | 一种针对组织质谱成像结果识别并划分细胞边界的方法 |
US11990327B2 (en) | 2022-02-18 | 2024-05-21 | Shimadzu Corporation | Method, system and program for processing mass spectrometry data |
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JPWO2020230397A1 (ja) * | 2019-05-10 | 2021-12-09 | 株式会社島津製作所 | 理論質量の外れ値検出方法 |
JP7095805B2 (ja) | 2019-05-10 | 2022-07-05 | 株式会社島津製作所 | 理論質量の外れ値検出方法 |
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