JP2015505241A - 生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット - Google Patents
生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット Download PDFInfo
- Publication number
- JP2015505241A JP2015505241A JP2014546191A JP2014546191A JP2015505241A JP 2015505241 A JP2015505241 A JP 2015505241A JP 2014546191 A JP2014546191 A JP 2014546191A JP 2014546191 A JP2014546191 A JP 2014546191A JP 2015505241 A JP2015505241 A JP 2015505241A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- probe
- sample
- hybridization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161569656P | 2011-12-12 | 2011-12-12 | |
| US61/569,656 | 2011-12-12 | ||
| PCT/US2012/069178 WO2013090386A2 (en) | 2011-12-12 | 2012-12-12 | Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015505241A true JP2015505241A (ja) | 2015-02-19 |
| JP2015505241A5 JP2015505241A5 (cg-RX-API-DMAC7.html) | 2016-01-28 |
Family
ID=47436256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014546191A Pending JP2015505241A (ja) | 2011-12-12 | 2012-12-12 | 生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9145584B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2802671B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2015505241A (cg-RX-API-DMAC7.html) |
| CN (2) | CN105385757B (cg-RX-API-DMAC7.html) |
| BR (1) | BR112014014307A2 (cg-RX-API-DMAC7.html) |
| DK (1) | DK2802671T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2013090386A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112014014307A2 (pt) | 2011-12-12 | 2019-09-24 | Cellay Inc | métodos e kits para detecção in situ à temperatura ambiente de um ácido nucleico alvo em uma amostra biológica |
| US20140161972A1 (en) * | 2012-12-09 | 2014-06-12 | National Sun Yat-Sen University | Method for forming conductive film at room temperature |
| EP3066214B1 (en) | 2013-11-07 | 2019-10-02 | Cellay, Inc. | Methods and kits for detecting a prostate carcinoma and predicting disease outcomes for prostate cancers |
| US11021739B2 (en) * | 2015-02-04 | 2021-06-01 | Alma Mater Studiorum Uneverseta Di Bologna | Additive for accelerating hybridization |
| CN108779490A (zh) * | 2016-01-08 | 2018-11-09 | 雅培分子公司 | 包含硫氰酸胍的杂交缓冲液 |
| US10577645B2 (en) * | 2016-03-18 | 2020-03-03 | Norgen Biotek Corp. | Methods and kits for improving global gene expression analysis of human blood, plasma and/or serum derived RNA |
| US11584924B2 (en) | 2016-08-24 | 2023-02-21 | Infusion Tech | Method for concentrating microorganism or extracting nucleic acid using DTBP |
| US11287422B2 (en) | 2019-09-23 | 2022-03-29 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| KR102673492B1 (ko) * | 2019-09-23 | 2024-06-10 | 엘리먼트 바이오사이언스, 인크. | 세포적으로 처리가능한 핵산 서열분석 방법 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006523100A (ja) * | 2003-04-03 | 2006-10-12 | モナリザ メディカル, リミテッド | 経子宮頸細胞を使用する非侵襲的出生前遺伝子診断 |
| JP2008535508A (ja) * | 2005-04-14 | 2008-09-04 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング | 腫瘍組織における増大したコピー数のegfr遺伝子に基づく抗egfr抗体療法 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5753433A (en) * | 1909-12-05 | 1998-05-19 | Boehringer Mannheim Gmbh | Method for the sensitive detection of nucleic acids |
| US5753437A (en) | 1987-10-13 | 1998-05-19 | The United States Of America As Represented By The Department Of Health & Human Services | Method of diagnosing cancer susceptibility or metastatic potential |
| US5082830A (en) | 1988-02-26 | 1992-01-21 | Enzo Biochem, Inc. | End labeled nucleotide probe |
| US5714327A (en) | 1990-07-19 | 1998-02-03 | Kreatech Diagnostics | Platinum-containing compounds, methods for their preparation and applications thereof |
| NL9001639A (nl) | 1990-07-19 | 1992-02-17 | Amc Amsterdam | Pt-houdende verbinding, werkwijze voor de bereiding ervan, alsmede toepassing van dergelijke verbindingen. |
| US5719024A (en) | 1993-08-18 | 1998-02-17 | Applied Spectral Imaging Ltd. | Method for chromosome classification by decorrelation statistical analysis and hardware therefore |
| NZ307633A (en) | 1995-05-09 | 2000-01-28 | Kreatech Biotechnology Bv | Methods for the production of platinum-based linkers between labels and bio-organic molecules, for labelling bio-organic molecules, for detecting |
| US6780603B1 (en) | 1995-07-21 | 2004-08-24 | Regents Of The University Of Minnesota | Analysis of alpha integrins for the diagnosis of diabetic nephropathy |
| JP2001501629A (ja) | 1996-10-04 | 2001-02-06 | ノボ ノルディスク アクティーゼルスカブ | N―置換アザ複素環式化合物 |
| US20020197656A1 (en) | 1999-12-17 | 2002-12-26 | Ronghao Li | Cell arrays and the uses thereof |
| US20040115692A1 (en) | 2000-04-03 | 2004-06-17 | Cytyc Corporation | Methods, compositions and apparatuses for detecting a target in a preservative solution |
| US6617148B2 (en) | 2000-06-29 | 2003-09-09 | E. I. Du Pont De Nemours And Company | Natural promoters for gene expression and metabolic monitoring in bacillus species |
| EP1373572B1 (en) | 2001-03-02 | 2006-07-26 | Stratagene California | Compositions and methods using platinum compounds for nucleic acid labeling |
| CA2504830A1 (en) | 2002-11-05 | 2004-05-21 | Roskamp Research Llc | Organic anion transport polypeptide related protein-4 (oatprp4) gene in tourette syndrome and related disorders |
| US6995020B2 (en) * | 2003-07-21 | 2006-02-07 | Aureon Laboratories, Inc. | Methods and compositions for the preparation and use of fixed-treated cell-lines and tissue in fluorescence in situ hybridization |
| DE602005022714D1 (de) | 2004-06-03 | 2010-09-16 | Advandx Inc | Hybridisierung von pna-sonden in alkohollösungen |
| US20090215643A1 (en) * | 2007-07-26 | 2009-08-27 | Cellay, Llc | Highly Visible Chromosome-Specific Probes and Related Methods |
| US20090036664A1 (en) | 2007-07-31 | 2009-02-05 | Brian Jon Peter | Complex oligonucleotide primer mix |
| WO2009041917A1 (en) * | 2007-09-28 | 2009-04-02 | Agency For Science, Technology And Research | Method of electrically detecting a nucleic acid molecule |
| CN101363048B (zh) * | 2008-08-29 | 2011-11-23 | 芮屈生物技术(上海)有限公司 | 一种nkx2-8基因的原位杂交检测试剂盒及其检测方法和应用 |
| BR112012030813A2 (pt) | 2010-06-03 | 2017-06-20 | Cellay Inc | "métodos e kits para detecção in situ de sequências de nucleotídeo" |
| BR112014014307A2 (pt) | 2011-12-12 | 2019-09-24 | Cellay Inc | métodos e kits para detecção in situ à temperatura ambiente de um ácido nucleico alvo em uma amostra biológica |
-
2012
- 2012-12-12 BR BR112014014307A patent/BR112014014307A2/pt not_active IP Right Cessation
- 2012-12-12 WO PCT/US2012/069178 patent/WO2013090386A2/en not_active Ceased
- 2012-12-12 CN CN201510835512.4A patent/CN105385757B/zh not_active Expired - Fee Related
- 2012-12-12 US US13/712,334 patent/US9145584B2/en not_active Expired - Fee Related
- 2012-12-12 EP EP12806823.6A patent/EP2802671B1/en not_active Not-in-force
- 2012-12-12 DK DK12806823.6T patent/DK2802671T3/en active
- 2012-12-12 JP JP2014546191A patent/JP2015505241A/ja active Pending
- 2012-12-12 CN CN201280069016.7A patent/CN104145027A/zh active Pending
-
2015
- 2015-09-03 US US14/844,823 patent/US10400275B2/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006523100A (ja) * | 2003-04-03 | 2006-10-12 | モナリザ メディカル, リミテッド | 経子宮頸細胞を使用する非侵襲的出生前遺伝子診断 |
| JP2008535508A (ja) * | 2005-04-14 | 2008-09-04 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング | 腫瘍組織における増大したコピー数のegfr遺伝子に基づく抗egfr抗体療法 |
Non-Patent Citations (1)
| Title |
|---|
| J. EXP. CLIN. CANCER RES., vol. 30:38, JPN6016035352, 2011, ISSN: 0003399866 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2802671B1 (en) | 2018-07-18 |
| CN105385757A (zh) | 2016-03-09 |
| BR112014014307A2 (pt) | 2019-09-24 |
| WO2013090386A2 (en) | 2013-06-20 |
| EP2802671A2 (en) | 2014-11-19 |
| US20150368701A1 (en) | 2015-12-24 |
| WO2013090386A3 (en) | 2013-08-22 |
| CN104145027A (zh) | 2014-11-12 |
| US9145584B2 (en) | 2015-09-29 |
| CN105385757B (zh) | 2020-04-03 |
| DK2802671T3 (en) | 2018-11-05 |
| US10400275B2 (en) | 2019-09-03 |
| US20130149705A1 (en) | 2013-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK2576830T3 (en) | Methods for in situ detection of nucleotide sequences | |
| US9145584B2 (en) | Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample | |
| RU2505609C2 (ru) | Композиции и способы гибридизации | |
| CA2116214C (en) | In situ hybridization method | |
| EP2914737B1 (en) | Methods and kits for performing in situ hybridization | |
| US20240084395A1 (en) | Single-stranded oligonucleotide probes for chromosome or gene copy enumeration | |
| AU2002240180A1 (en) | Hybridization buffers using low molecular weight dextran sulfate and methods for their use | |
| US20160046984A1 (en) | Robust Detection of Nucleic Acids in Situ | |
| Kearney et al. | Fluorescence in situ hybridization for cancer-related studies | |
| US20230093993A1 (en) | Methods And Kits For Detecting A Prostate Carcinoma And Predicting Disease Outcomes For Prostate Cancers | |
| Knoll et al. | In situ hybridization and detection using nonisotopic probes | |
| Sterchi et al. | Molecular pathology 21 | |
| GOSDEN et al. | YOSHIRO SHIBASAKI |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20151204 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20151204 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20160913 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20161212 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20170522 |