JP2015199700A - Neutral fat absorption-suppressing composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a neutral fat absorption-suppressing composition which highly works for reducing a particle diameter of bile acid micelle and is capable of suppressing absorption of neutral fat into a body.SOLUTION: A neutral fat absorption-suppressing composition of the present invention can effectively suppress reduction of a particle diameter of bile acid micelle by containing polydextrose. A composition of the present invention is a mixture containing polydextrose and one type, two types or more of polymerizable monomers being a raw material thereof as a polydextrose source, and it is preferable to use the mixture having the viscosity of 50 mass/volume% aqueous solution at 25°C is 15 mPa s or more. Further, it is also preferable to use the mixture containing 50 mass% or more of dietary fiber as the polydextrose source.

Description

  The present invention relates to a neutral fat absorption inhibitory composition, and more particularly to a neutral fat absorption inhibitory composition that suppresses in vivo absorption of neutral fat by suppressing particle size reduction of bile acid micelles.

  Conventional neutral fat absorption suppression technology has been mainly based on lipase inhibition. Lipase is contained in the pancreatic juice secreted into the digestive tract, and is an important digestive enzyme that breaks down triglycerides into fatty acids and monoglycerides that are easily absorbed. By inhibiting the activity of this lipase, digestion of triglycerides can be inhibited and absorption can be suppressed. For example, orlistat is a drug that shows fat absorption inhibition using lipase inhibition as a mechanism of action.

  However, a problem with lipase inhibitors is that fatty stool appears as a side effect. When lipase is inhibited, the fat taken from the meal is excreted as stool without being digested, so that there is a problem that the QOL is remarkably lowered, such as oil coming out from the anus during the release. .

  The suppression of fat absorption by controlling the particle size of bile acid micelles does not inhibit the function of lipase, and fat is decomposed into fatty acids and monoglycerides, so there is no side effect of excreting undigested oil like fatty stool. On the other hand, the absorption of fat via bile acid micelles can be delayed by controlling the bile acid micelle particle size and suppressing the decrease in the particle size. That is, by controlling the particle size of bile acid micelles and suppressing the decrease thereof, fat stool, which is a side effect of lipase inhibitors, can be suppressed, and fat absorption can be suppressed.

  A micelle is a sphere formed by collecting a plurality of such molecules in a state where the polar hydrophilic portion of an amphiphilic molecule such as a surfactant extends outward and the nonpolar hydrophobic portion extends inward. Refers to the structure. For example, when water and oil are mixed together with a surfactant, the lipophilic part of the surfactant is oriented with the oil inside and the hydrophilic part is oriented toward the interface with water to form a micelle structure. Among micelles, bile acid micelles are micelles formed in the presence of bile acids, and are usually formed in vivo by bile acids, fatty acids, monoglycerides, and the like. These bile acid micelles are known to participate in the lipid digestion and absorption process as follows, for example, in vivo.

  Many of the fats in food are chemically stable neutral fats, or triglycerides. It is known that neutral fat taken into the body as a meal is digested and absorbed as follows. First, neutral fat is emulsified with bile in the duodenum. Next, it is decomposed into monoglycerides and fatty acids by the action of pancreatic lipase contained in the pancreatic juice. Monoglycerides and fatty acids form bile acid micelles by contact with bile acids secreted into the intestine, and are absorbed through the bile acid micelles. It is known that bile acid micelles are more easily absorbed as the particle size is smaller. Therefore, it is considered that a composition that controls the particle size of bile acid micelles and suppresses the decrease in particle size can suppress fat absorption.

  So far, it has been reported that indigestible dextrin, which is a kind of water-soluble dietary fiber, suppresses the decrease in the particle size of bile acid micelles and suppresses fat absorption (Non-patent Document 1).

Journal of Health Science, 55 (5), 838-844 (2009)

However, it is difficult to say that the effect of suppressing the particle size reduction of bile acid micelles by indigestible dextrin is sufficient.
The object of the present invention is to suppress the decrease in the particle size of bile acid micelles, which has a higher action than indigestible dextrin, and effectively absorbs neutral fat into the body by delaying fat absorption. An object of the present invention is to provide a neutral fat absorption suppressing composition that can be suppressed.

  The present invention provides a neutral fat absorption inhibiting composition containing polydextrose as an active ingredient.

  According to the composition of the present invention, the particle size reduction of bile acid micelles can be effectively suppressed, and it can be effective in suppressing the absorption of neutral fat into the body by delaying the absorption of fat.

FIG. 1 is a graph showing the results of a bile acid micelle particle size reduction suppression test in Examples and Comparative Examples. FIG. 2 is a graph showing the results of a test for suppressing the decrease in the particle size of bile acid micelles when the amount of dietary fiber was changed in the examples. FIG. 3 is a graph showing the results of a particle size reduction inhibition test of bile acid micelles when the viscosity of a 50 mass / volume% aqueous solution of a polydextrose mixture was changed in the examples. FIG. 4 is a graph showing the results of a bile acid micelle particle size reduction inhibition test when the pH of the composition of the present invention was changed in the examples.

  Hereinafter, the neutral fat absorption inhibiting composition of the present invention will be described based on preferred embodiments thereof. The present invention is intended to suppress fat absorption by suppressing particle size reduction of bile acid micelles, and is also used as a composition for suppressing particle size reduction of bile acid micelles. In addition, the present invention aims to promote the excretion of lipids outside the body by suppressing the decrease in the particle size of bile acid micelles. Hereinafter, these compositions are also simply referred to as the composition of the present invention.

  The composition of this invention suppresses the particle size fall of a bile acid micelle effectively by containing polydextrose.

  Polydextrose is a water-soluble and indigestible polysaccharide that has a structure in which glucose is polymerized in a branched manner with α- or β-type 1-2, 1-3, 1-4, or 1-6 bonds. And some of them work as dietary fiber. Polydextrose is produced, for example, by mixing glucose, sorbitol, and citric acid at a mass ratio of about 89: 10: 1, or glucose, sorbitol, and phosphoric acid at a mass ratio of about 90: 10: 0.1, and then polymerizing them under high temperature conditions. Is done. The amount of polydextrose is measured by the methods described in the US Federal Chemicals Codex and the separate sheet of July 21, 2006, from Shokuyasu No. 0721001.

  In the present invention, as a polydextrose source, a mixture containing polydextrose and one or more polymerizable monomers as raw materials as impurities (hereinafter also referred to as a polydextrose mixture) may be used. Alternatively, only polydextrose from which impurities have been removed may be used. Examples of the polymerizable monomer contained in the polydextrose mixture include the aforementioned glucose, which is a raw material for producing polydextrose, and sorbitol. That is, the polydextrose mixture may be composed of, for example, polydextrose, glucose and sorbitol. The polydextrose mixture is usually solid. The polydextrose mixture preferably has a water content of, for example, 5% by mass or less, particularly 4% by mass or less.

  The total amount of glucose and sorbitol in the polydextrose mixture is preferably 10% by mass or less, and more preferably 6% by mass or less. The smaller the total amount of glucose and sorbitol in the polydextrose mixture, the better. However, it is preferably 1% by mass or more from the viewpoint of the production cost of polydextrose. The contents of glucose and sorbitol can be measured by various known quantitative methods.

  Further, in the polydextrose mixture, the ratio (A) / (B) of the total amount of (A) dietary fiber and (B) glucose and sorbitol described later in the mixture is 8.3 or more, particularly 12.5 or more. preferable.

  Polydextrose and polydextrose mixtures contain dietary fiber. Dietary fiber is an indigestible component that is not digested by human digestive enzymes among the components contained in food. As described above, polydextrose is a polysaccharide obtained by polymerizing monosaccharides such as glucose, and among these, those having a molecular weight of a certain level or more are considered to be dietary fibers.

  In the present invention, when the polydextrose mixture described above is used, the amount of dietary fiber in the polydextrose mixture is 50% by mass or more, so that sweetness and miscellaneous taste due to impurities derived from the raw material can be reduced, and the micelle particle size is reduced. From the viewpoint of effectively suppressing the above. The amount of dietary fiber in the mixture is more preferably 60% by mass or more, still more preferably 65% by mass or more, and particularly preferably 70% by mass or more. Moreover, although the amount of dietary fiber in the said mixture is so preferable that it is high, it is advantageous at the point of manufacturing cost that it is 95 mass% or less, for example.

  In the present invention, in order to keep the amount of dietary fiber contained in the polydextrose mixture within the above range, after producing polydextrose by the above-described method, the reaction product obtained by the polymerization may be purified by a known method. That's fine.

  In addition, the amount of dietary fiber contained in the polydextrose mixture is the enzyme-HPLC method ("Analytical methods for nutritional components, etc. in nutrition labeling standards", April 26, 1999, Eishin No. 13, 8 dietary fiber) Measure according to. Specifically, it is performed as follows.

First, 500 mg of a sample is accurately measured, and 50 ml of 0.08 mol / l phosphate buffer (pH 6.0 ± 0.5) is added. To this, 0.1 ml of a thermostable α-amylase (Sigma: EC 3.2.1.1 Bacillus icheniformis derived) solution is added, placed in boiling water, and allowed to stand for 30 minutes with stirring every 10 minutes. After cooling, 0.275 mol / l sodium hydroxide solution is added to adjust the pH to 7.5 ± 0.1. Add 0.1 ml of protease (from Sigma: EC 3.4.21.62 Bacillus icheniformis ) solution and react for 30 minutes while shaking in a water bath at 60 ± 2 ° C. After cooling, 0.325 mol / l hydrochloric acid is added to adjust the pH to 4.3 ± 0.3. Add 0.1 ml of amyloglucosidase (Sigma: EC 3.2.13 Aspergillus niger ) solution, and allow to react for 30 minutes while shaking in a 60 ± 2 ° C. water bath. Immediately after completion of the above enzyme treatment, it is heated in a boiling water bath for 10 minutes, then cooled, dispensed into 50 ml centrifuges, centrifuged for 10 minutes, and the supernatant is recovered. Add 10 ml of pure water to the residue, stir well, centrifuge again to collect the supernatant, and use these as the enzyme treatment solution. The total amount of the enzyme treatment solution was passed through a column (glass tube 20 mm × 300 mm) packed with 50 ml of ion exchange resin (OH type: H type = 1: 1) at a flow rate of 80 ml / hr, and the effluent was further passed through pure water The total volume is about 250 ml. This solution is concentrated by a rotary evaporator, and the whole volume is made up to 50 ml with pure water. Filter through a membrane filter with a pore size of 0.45 μm to make a test solution. Glycerin is prepared to 5 mg / ml, 10 mg / ml, and 15 mg / ml with pure water to obtain a standard solution.

  Next, liquid chromatography is performed on 20 μl of the test solution and standard solution, and the peak area values of the dietary fiber fraction of the test solution and glycerin of the standard solution are measured.

The analysis conditions for liquid chromatography are as follows.
Detector: Differential refractometer Column: ULTRON PS-80N (φ8.0 × 300 mm, Shimadzu GL)
Column temperature: 50 ° C
Mobile phase: Ultrapure water Flow rate: 0.5 ml / min

The amount of dietary fiber is calculated from the following equation by preparing a calibration curve from the standard solution concentration and area, obtaining the glycerol equivalent from the calibration curve.
Amount of dietary fiber (%) = [glycerol equivalent (mg / ml) / sampled amount (mg)] × f1 × 50 (ml) × 100
(In the above formula, f1 is the sensitivity ratio (0.82) of the peak area of glycerin and glucose.)

In the present invention, as a polydextrose source, a mixture containing polydextrose and one or more polymerizable monomers as raw materials thereof, and the viscosity of an aqueous solution having a specific concentration at 25 ° C. is a specific value or more The present inventors have found that the use of the mixture as described above enables the composition of the present invention to more effectively suppress the particle size reduction of bile acid micelles. Specifically, the polydextrose mixture preferably has a 50 mass / volume% aqueous solution viscosity of 15 mPa · s or higher at this temperature.
Further, the viscosity of the 50 mass / volume% aqueous solution in the polydextrose mixture is preferably as high as possible, but is preferably 25 mPa · s or less from the viewpoint of the production cost of the polydextrose mixture.
Here, the concentration of the aqueous solution of the polydextrose mixture being “X mass / volume%” means that the mass of the polydextrose mixture contained in 100 ml of the aqueous solution is Xg.

The viscosity of the aqueous solution can be measured using an LVT RVT HAT HBT analog viscometer (manufactured by Brookfield) and specifically by the following procedure.
As an example, a No. 1 spindle is placed on the main body, and the spindle is immersed in a prepared position in 80 mL of a 50% mass / volume sample prepared. Set the rotation speed to 50 rpm, check that the pointer points to 0 mPa · s on the scale, and turn on the motor switch. Viscosity is calculated from the numerical value indicated by the pointer 1 minute after the start of spindle rotation and recorded.

  In order to obtain a polydextrose mixture in which the viscosity of the aqueous solution is in the specific range, the molecular weight and degree of polymerization of the polydextrose used may be adjusted according to fractionation and polymerization conditions, or may be purified by a known method. .

Further, the composition of the present invention is a solid substance, and the pH of 25 ° C. when it is an aqueous solution having a concentration of 20% by mass / volume is within a specific range, the composition of the present invention enables the bile acid micelle particles It is preferable because a decrease in diameter can be effectively suppressed. Specifically, when the composition of the present invention is an aqueous solution having a concentration of 20% by mass / volume, the pH at the above temperature is preferably higher than 2.0 and lower than 8.0, and preferably 2.1 or more and 7 It is more preferably 0.9 or less, further preferably 2.5 or more and 7.5 or less, and most preferably 3.0 or more and 7.0 or less. Such a polydextrose mixture can be obtained, for example, by purifying the above-mentioned polymer of glucose and sorbitol by a known method.
Moreover, in this invention, it is also preferable to use the polydextrose mixture whose pH in 25 degreeC when it is set as 20 mass / volume% aqueous solution is the said range as a polydextrose source.
Furthermore, it is also preferable that the composition of the present invention is a liquid composition in which the pH at 25 ° C. is adjusted to the above range from the viewpoint of effectively suppressing the decrease in the particle size of bile acid micelles. In order to adjust the pH when the composition of the present invention is liquid to the above range, a polydextrose or polydextrose mixture having a pH in the above range when dissolved or dispersed in water can be used. , PH adjusting agent and the like may be added. The liquid composition here is preferably one obtained by dissolving or dispersing polydextrose or a polydextrose mixture in water. The liquid composition contains other optional components as required.
The pH of the aqueous solution of the composition of the present invention and the polydextrose mixture and the pH of the liquid composition can be measured by the methods described in Examples below.

  In addition to polydextrose produced by the above method, polydextrose may also include derivatives of the polydextrose. Examples of such derivatives include hydrogenated or reduced polydextrose obtained by hydrogenating polydextrose produced by the above method. Further, polydextrose produced by neutralizing the polydextrose produced by the above method with a base such as potassium hydroxide is also included in polydextrose.

  The composition of the present invention can be taken orally. In this case, the dosage form of the composition is, for example, powder, granule, granule, tablet, rod, plate, block, solid , Round, liquid, candy, paste, cream, capsule like hard capsule or soft capsule, caplet, tablet, gel, jelly, gummy, wafer, biscuit, cookie, chewable , Syrup form, stick form and the like.

  The composition of the present invention may be composed only of polydextrose or polydextrose mixture, and may contain other components in addition to these. Other ingredients include, for example, vitamins, proteins, water-soluble dietary fibers other than polydextrose, oligosaccharides, minerals, mucopolysaccharides, dairy products, soy milk products, plants or processed plant products, lactic acid bacteria, natto bacteria, butyric acid Microorganisms such as fungi, bacilli and yeast can be blended. Furthermore, sugars, sweeteners, acidulants, colorants, thickeners, brighteners, production agents, antioxidants, pH adjusters and the like that are usually used in the food field as needed can be mentioned. Other ingredients include various excipients, binders, lubricants, stabilizers, diluents, extenders, thickeners, emulsifiers, colorants, flavors, food additives, seasonings. And so on. The content of other components can be appropriately selected according to the form of the composition of the present invention.

  The content of polydextrose in the composition of the present invention varies depending on the use and dosage form of the composition, the required level of effect, etc., but generally the proportion of polydextrose in the solid content of the composition of the present invention is 3.0. The mass is preferably from 100% by mass to 100% by mass, and more preferably from 3.3% by mass to 100% by mass.

  When the composition of the present invention is a solution in which polydextrose is dissolved in a liquid, the concentration of polydextrose in the composition is preferably 10 g / L or more and 900 g / L or less, preferably 10 g / L or more and 800 g. / L or less is more preferable.

  Further, when the composition of the present invention is obtained by dissolving a polydextrose mixture in a liquid such as water, the concentration of the polydextrose mixture in the composition is 0.1 mass / volume% or more and 50 mass / volume%. Is preferably 0.5 mass / volume% or more and 40 mass / volume% or less, more preferably 1 mass / volume% or more and 30 mass / volume% or less.

  The amount of polydextrose in the composition of the present invention is preferably 1 g or more and 30 g or less, more preferably 2 g or more and 20 g or less, and more preferably 3 g or more and 12 g or less as polydextrose. It is more preferable that

  When the composition of the present invention is orally ingested, the particle size reduction of bile acid micelles can be suppressed in the digestive tract.

  The bile acid micelle specifically refers to a micelle formed by a decomposition product of fat in the presence of bile acid. Bile acid is a generic name for compounds having a colanic acid skeleton, which are steroid derivatives widely found in mammalian bile. Examples of bile acids include COOH-type bile acids, conjugated bile acids, and salts thereof. Examples of the COOH-type bile acids include cholic acid, chenodeoxycholic acid, hyocholic acid, 5α-cypurinol, deoxycholic acid, lithocholic acid, ursodeoxycholic acid, hyodeoxycholic acid and the like. Examples of conjugated bile acids include those in which the carboxyl group of COOH-type bile acids is amide-bonded with taurine or glycine, and examples include glycocholic acid and taurocholic acid. Examples of the COOH-type bile acid and conjugated bile acid salts described above include sodium salts and potassium salts. In the present invention, the bile acid preferably contains a conjugated bile acid or a salt thereof. Moreover, it is preferable that a bile acid micelle contains a fatty acid and a monoglyceride, and it is especially preferable that a fatty acid and 2-monoglyceride are included. The fatty acid may be a saturated fatty acid or an unsaturated fatty acid.

  Such a composition of the present invention can effectively suppress the absorption of fatty acids and monoglycerides in the small intestinal epithelium by suppressing the decrease in the particle size of bile acid micelles in the digestive tract. Moreover, the discharge | emission of a lipid outside a body can be accelerated | stimulated by suppressing the particle size fall of the bile acid micelle in a digestive tract. Therefore, the composition of this invention is effective for the use which suppresses absorption of a neutral fat.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the scope of the present invention is not limited to such examples. Hereinafter, unless otherwise specified, “%” represents mass%, and “part” represents mass part.
In addition, the following dietary fiber amount and viscosity were measured by the method mentioned above.

[Example 1]
As polydextrose, the following polydextrose mixture A was dissolved in distilled water to obtain a 20 mass / volume% aqueous solution sample.
Polydextrose mixture A:
Viscosity of 50 mass / volume% aqueous solution at 25 ° C. is 20 mPa · s, dietary fiber content is 80.16 mass%, ratio of (A) dietary fiber content and (B) total amount of glucose and sorbitol (A) / (B) is 18.6.

[Comparative Example 1]
Instead of the aqueous solution of the polydextrose mixture, an indigestible dextrin-containing material (the amount of indigestible dextrin was 94.4% by mass) was dissolved in distilled water to obtain a 20% by mass / aqueous solution sample.

  The aqueous solution samples of Example 1 and Comparative Example 1 obtained above were subjected to the following <micelle formation test>. Further, distilled water was used instead of the aqueous solution sample, and a similar <micelle formation test> was performed as Reference Example 1 (negative control).

<Micelle formation test>
0.0125 g of sodium taurocholate and 0.0195 g of 2-monoolein were weighed into a 1.5 mL Eppendorf tube.
500 μL of the aqueous solution sample (Example 1 or Comparative Example 1) or distilled water (Reference Example 1) and 34.25 μL of oleic acid were added to the 1.5 mL Eppendorf tube, and the mixture was stirred by vortexing for 10 seconds. The obtained mixture was homogenized at room temperature for 3 minutes with a homogenizer (manufactured by Microtech Nichion Co., Ltd., Histocolon NS-310EII). Here, the strength of the homogenizer was set to 4. After 30 seconds of the homogenization treatment, 2 μL was sampled from the bottom of the Eppendorf tube, and diluted by adding to 1 mL of 0.15% SDS solution that had been heated to 37 ° C. (1 hour or more) in advance. After dilution, the average micelle diameter of the diluted solution was measured using “Zeta Sizer Nano s” manufactured by Malvern, and defined as “average diameter after 0 minutes of homogenization treatment”. The measurement temperature was 37 ° C. Even after 60, 120, 180 minutes of the homogenization treatment, the same sampling and dilution were performed from the homogenized liquid, and the average micelle diameter was measured. The average diameter after minutes ”. Both the homogenized liquid and the diluted liquid were allowed to stand at 37 ° C. until the measurement after 180 minutes was completed. For each average value of the average diameter after 60, 120, and 180 minutes after homogenization, the ratio when the average value of the average diameter after 0 minutes after homogenization was 100 was calculated. The result is shown in FIG.

  As shown in FIG. 1, in Example 1 using polydextrose, the average particle size of bile acid micelles was decreased compared to Comparative Example 1 using indigestible dextrin and Reference Example 1 being a negative control. Suppressed. Therefore, it can be seen that the composition of the present invention using polydextrose effectively suppresses the particle size reduction of bile acid micelles.

[Examples 2 to 4]
Three types of polydextrose mixtures having different production conditions from the polydextrose mixture A used in Example 1 were produced. Table 1 shows the dietary fiber content of these polydextrose mixtures measured by the above method. The obtained polydextrose mixture was dissolved in distilled water to obtain a 20 mass / volume% aqueous solution sample.

  The result of having used the sample of Examples 2-4 for said <micelle formation test> is shown in FIG. In FIG. 2, the result of using distilled water as a negative control is also shown as Reference Example 2 as in FIG.

[Examples 5 to 8]
Four polydextrose mixtures having different production conditions from the polydextrose mixture used in Example 1 were produced. With respect to these polydextrose mixtures, the viscosity (mPa · s) of 50 mass / volume% aqueous solution was measured by the above method. The results are shown in Table 2. Reference Example 3 in Table 2 is distilled water.

  Each polydextrose mixture used in Examples 5 to 8 was used as a 20 mass / volume% aqueous solution sample, and these were subjected to the above-described <micelle formation test>. About each polydextrose mixture used in Examples 5-8, the viscosity (mPa * s) of 50 mass / volume% aqueous solution was used for the horizontal axis, and the 20 mass / volume% aqueous solution sample was used for <micelle formation test>. FIG. 3 is a graph in which the obtained average particle diameter (ratio of average particle diameter after 180 minutes after homogenization,% when the average particle diameter after 0 minutes after homogenization is 100) is plotted on the vertical axis. . FIG. 3 also shows the viscosity of distilled water used as a negative control (Reference Example 3) and the average particle diameter obtained by subjecting the distilled water to the <micelle formation test>.

  From the results shown in FIG. 2, it can be seen that when a polydextrose mixture having a dietary fiber content of 50% by mass or more is used, a decrease in the particle size of bile acid micelles is more effectively suppressed. Further, from the results shown in FIG. 3, even when a polydextrose mixture having a viscosity of an aqueous solution of 50% by mass / volume of 15 mPa · s or more is used, the decrease in the particle size of bile acid micelles is further effectively suppressed. I understand that.

Example 9
A polydextrose mixture (dietary fiber content 70%) was dissolved in distilled water to obtain a liquid composition (polydextrose mixture concentration: 20 mass / volume%). At this time, the pH of the liquid composition was adjusted to 2.0 using citric acid. The pH was measured by the following method.

<Measurement method of pH>
The measurement was performed at 25 ° C. using “Seven Easy” manufactured by METTLER TOLEDO as a pH meter.

Example 10
A liquid composition (polydextrose mixture concentration: 20 mass / volume%, pH 3.0) was obtained in the same manner as in Example 9, except that the pH was adjusted to 3.0.

Example 11
A polydextrose mixture (dietary fiber content 70%) was dissolved in distilled water to obtain a liquid composition (polydextrose mixture concentration: 20 mass / volume%). The pH of this liquid composition was measured as described above, and it was pH 5.36.

Example 12
A liquid composition (polydextrose mixture concentration: 20 mass / volume%, pH 7.0) was obtained in the same manner as in Example 9, except that the pH was adjusted to 7.0 using sodium hydroxide.

Example 13
A liquid composition (polydextrose mixture concentration: 20 mass / volume%, pH 8.0) was obtained in the same manner as in Example 10 except that the pH was adjusted to 8.0 using sodium hydroxide.

  Each of the liquid compositions obtained in Examples 9 to 13 was used as an aqueous solution sample and subjected to the above-described <micelle formation test> to obtain an average particle size (uniform when the average particle size 0 minutes after homogenization was 100) The ratio of the average particle diameter 180% after the conversion was obtained. FIG. 4 is a graph in which the pH of the liquid composition is plotted on the horizontal axis and the vertical axis is plotted on the average particle diameter.

  From the results shown in FIG. 4, when a polydextrose mixture and a solid composition having a 20 mass / volume% aqueous solution with a pH of more than 2.0 and less than 8.0 are used, the particle size of bile acid micelles It can be seen that the reduction is effectively suppressed. Further, when the liquid compositions of Examples 10, 11 and 12 having a pH of more than 2.0 and less than 8.0 were used, Example 9 having a pH of 2.0 or less and Example having a pH of 8.0 or more It can be seen that the decrease in the particle size of the bile acid micelles is more effectively suppressed as compared to the composition of 13.

(Formulation Example 1: Powder)
Excipients necessary for production were added to polydextrose (80% dietary fiber content) to prepare a composition of the present invention in the form of a powder in which polydextrose was 95%.

(Formulation Example 2: Tablet)
Mix and tablet 5 parts of polydextrose (50% dietary fiber), 5 parts of vitamin B ₁ , 12 parts of vitamin B ₆ , 4 parts of calcium stearate, 30 parts of maltose and the rest of hydroxypropylcellulose The composition of the present invention was prepared in the form of a tablet.

(Formulation Example 3: Granules)
The composition of the present invention in the form of granules by mixing and granulating 90 parts of polydextrose (dietary fiber content 70%), 5 parts of tea extract, some amount of fragrance, and the remainder of calcium stearate with 100 parts as a whole. Was prepared.

(Formulation Example 4: Capsule)
An embodiment of a capsule containing 100 parts as a whole and mixing 40 parts of polydextrose (dietary fiber amount 50%), 8 parts of lecithin and the remainder of olive oil as a content liquid and encapsulating it in a capsule shell The composition of the present invention was prepared.

(Formulation Example 5: Liquid formulation)
Total 100 parts, 1 part polydextrose (dietary fiber 75%) 1 part, vitamin B ₁ 1 part, fructose glucose liquid sugar 10 parts, citric acid 1 part, sodium benzoate 0.02 part, flavor preparation 2 parts, sucralose The composition of the present invention was prepared in the form of a liquid by mixing 0.05 part, 0.03 part acesulfame potassium, and the remaining purified water.

Claims (5)

  A neutral fat absorption-suppressing composition comprising polydextrose.   The neutral fat absorption suppression composition according to claim 1, wherein the neutral fat absorption suppression is due to suppression of a decrease in the particle size of bile acid micelles.   The polydextrose source is a mixture containing one or more polydextrose and a polymerizable monomer as a raw material thereof, and the viscosity of a 50 mass / volume% aqueous solution at 25 ° C. is 15 mPa · s or more. The neutral fat absorption inhibitory composition of Claim 1 or 2 using this mixture.   The polydextrose source is a mixture containing one or more polydextrose and a polymerizable monomer as a raw material thereof, and the mixture having a dietary fiber amount of 50% by mass or more is used. The neutral fat absorption inhibitory composition according to any one of 3 above.   The neutral fat according to any one of claims 1 to 4, which is a solid and has a pH of 25 ° C higher than 2.0 and lower than 8.0 when an aqueous solution having a concentration of 20% by mass / volume is obtained. Absorption suppression composition.