JP2015166317A - Blood sugar level elevation inhibitor, agent for suppressing blood pressure elevation, and extraction method of persimmon calyx - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a drug containing an active ingredient having a novel functionality except to stop hiccups or antibacterial activity, in a calyx part of persimmon which is usually destroyed, in order to aim at effective utilization of persimmon, and to provide an extraction method of the active ingredient.SOLUTION: The invention provide a blood sugar level elevation inhibitor and an agent for suppressing blood pressure elevation which contain alcohol extract from persimmon calyx as an active ingredient, and an extraction method of persimmon calyx comprising an extraction step of contacting persimmon calyx with alcohol to extract an active ingredient.

Description

  The present invention relates to a blood sugar level increase inhibitor, a blood pressure increase inhibitor, and a method for extracting sputum, which contain a sputum extract as an active ingredient.

  In general, persimmons are widely cultivated because edible fruits and processed foods using persimmon fruits are edible. When edible berries are used, the fruit portion is used after separating the berries other than fruits. Regarding the use of soot, for example, the following is known.

Patent Document 1 describes that cocoon pulp, peeled husk, and cocoon are mixed and processed at an arbitrary mixing ratio. Patent Document 2 describes that the fruit and cocoon of an immature cocoon are separated and the cocoon is removed.
The cocoon part of the cocoon is called cyte and is widely distributed as a traditional Chinese medicine called Sakaiyu. The main effect of Tsukuyu is to prevent shacking. Concerning Saito, Non-Patent Document 1 describes the effect of Saito on tonic clonic epilepsy.
Non-Patent Document 2 describes that ursolic acid and oleanolic acid, which are supposed to be contained in sputum, have antibacterial activity against caries.

JP 2006-288373 A JP 2008-63332 A

Minami E, Shibata H, Nomoto M, Fukuda T., “Effect of shitei-to, a traditional Chinese medicine formulation, on pentylenetetrazol-induced kindling in mice.” Phytomedicine. 2000 Mar; 7 (1): 69-72. Konishi et al., “Antimicrobial effect of sputum extract against caries-causing bacteria”, Japanese Dental Conservation Journal 51 (3), 299-307, 2008-06-30

  As described above, strawberries are used not only for fruits but also for skins and strawberries. However, since most cases are used for edible fruits, the most part of the cocoon part is discarded after the removal.

  Accordingly, an object of the present invention is to provide a drug containing an active ingredient having a new functionality other than shack prevention and antibacterial activity, and the active ingredient in a portion of the heel that is usually discarded in order to effectively use the heel. It is to provide an extraction method for extraction.

  As a result of studying further effective utilization of the cocoon part of the cocoon, the present inventors have found that a composition comprising an extract obtained by treating cocoon cocoon with alcohol as an active ingredient has an effect of suppressing an increase in blood glucose level and an increase in blood pressure. It was found to have an inhibitory action.

That is, in order to achieve the above object, the following inventions [1] to [3] are provided.
[1] A blood sugar level increase inhibitor comprising an alcoholic extract of salmon as an active ingredient.
[2] An antihypertensive agent comprising an alcoholic extract of salmon as an active ingredient.
[3] A method for extracting cocoons having an extraction step of extracting active ingredients by bringing the cocoons into contact with alcohol.

  The blood sugar level increase inhibitor and blood pressure increase inhibitor of the present invention have, as an active ingredient, a component contained in an extract obtained by extracting salmon with alcohol.

  A blood sugar level increase inhibitor, for example, has an α-glucosidase inhibitory activity that inhibits the action of α-glucosidase inhibitory activity in the intestine and inhibits the decomposition and absorption of sugar. Ingestion is expected to suppress an increase in blood glucose level.

  An antihypertensive agent, for example, has an angiotensin converting enzyme inhibitory activity that inhibits angiotensin converting enzyme (ACE) involved in an increase in blood pressure. By taking an effective amount of an antihypertensive agent, an increase in blood pressure can be achieved. It is expected to be suppressed.

  The koji used in the present invention is widely cultivated as an edible fruit and is easily available. Moreover, since the present invention effectively uses the wrinkle part of the wrinkle that is normally discarded, the material cost can be reduced. In addition, it can be used as it is for alcohol extraction, and can be easily handled because it can be cut and pulverized as necessary.

  In view of using the extract as a pharmaceutical or food, the alcohol used in the present invention is preferably ethanol or the like that is easy to obtain and has safety to the human body.

  That is, according to the present invention, a drug containing an active ingredient having new functionalities such as a blood glucose level increase suppressing action and a blood pressure increase suppressing action can be used effectively by simply discarding sputum that has been discarded. Can be easily obtained.

It is a photograph figure which shows the result of having performed the thin layer chromatography (TLC) analysis. It is a photograph figure which shows the result of having performed the thin layer chromatography (TLC) analysis. It is a figure which shows the result of having performed GC-MS analysis.

Hereinafter, embodiments of the present invention will be described with reference to the drawings.
The present invention uses a composition containing an extract obtained by treating cocoon with alcohol as an active ingredient for further effective utilization of the cocoon part of the cocoon as an inhibitor of blood sugar level increase or blood pressure increase. It is what you use.

  The part of the cocoon used in the present invention is a cocoon part other than the fruit. Persimmon refers to a persimmon that is not withered even after the petals have dried up and the fruit has ripened. There are no particular restrictions on the variety of koji used, and any taxonically belonging to Diospyros kaki can be used, but for reasons such as easy availability, it is named, for example, Fuyucho or Jiro It is good. Persimmons are young fruits harvested earlier than the normal harvest time (about 30 to 60 days after fruit set) or mature fruits harvested at the normal harvest time (about 150 to 180 days after fruit set) It is good to use. Fruits are removed from the harvested cocoons to collect cocoons, and the cocoons are used in a dry or non-dried state. What is necessary is just to perform a drying process with a well-known method and apparatus, when drying a straw. The collected cocoons are used in the form as they are or after being appropriately cut and crushed. In the case of cutting and pulverizing the scissors, the cutting and pulverizing process may be performed by a known method and apparatus.

  The obtained soot is treated with alcohol. The alcohol that can be used in the present invention is preferably a lower alcohol such as methanol, ethanol, propyl alcohol, isopropyl alcohol, butanol, and isobutanol. Moreover, you may utilize the mixed solution of these alcohol and water, the solution which mixed several alcohol mentioned above, and water, or the solution which mixed multiple said alcohol. The mixing ratio of alcohol and water is not particularly limited. In this specification, the case where 100% ethanol is used as the alcohol will be described.

  The method for extracting sputum according to the present invention includes an extraction step in which the active ingredient is extracted by bringing the sputum into contact with ethanol. In the extraction step, an extract (extract) is obtained by bringing ethanol into contact with the soot that has been appropriately subjected to the above-described treatment after collection and immersing, stirring, or refluxing.

As the extraction conditions, the extraction temperature can be arbitrarily set as long as it is not lower than the boiling point and lower than the freezing point of the solvent used for extraction, and preferably, the components contained in the soot are not easily denatured or lost due to the reaction. And it is good to set it as the temperature range from the normal temperature to the boiling point of a solvent with comparatively favorable extraction efficiency. Specifically, the extraction pressure is suitably set within a range of 1.0 to 2.5 kg / cm ² . The weight ratio of the solvent to the soot can be arbitrarily set, for example, soot: solvent in the range of 2: 1 to 1:50, but is not limited thereto. There is no particular limitation on the extraction time, and preferably several hours to several days.

  The extract of koji may use the extract (extract) obtained by the above-described solvent and extraction conditions as it is, but the extract can be further purified and used. As a purification method, specifically, a filtration method using a filter paper or a membrane filter, a liquid separation operation method, a distillation method, a sublimation method, a precipitation method, a recrystallization method, a centrifugal separation method, a vacuum concentration method, a pH adjustment, Although methods, such as salting out, are mentioned and 1 type or 2 types or more of these methods are selected, It is not limited to these methods.

  Further, the obtained extract (extract) may be diluted with an appropriate solvent, concentrated and used, or further dried under reduced pressure to obtain a dry powder or may be prepared in a paste form. .

  A composition obtained by adding water, alcohols, polyhydric alcohols, surfactants, acids, alkalis, thickeners, excipients, preservatives, etc. to the koji extract prepared in this way. It is also possible. The shape of the composition may be liquid, solid, powder, granule, pellet, gel, mucous, or the like.

  The composition can be administered as a pharmaceutical by oral, enteral, transmucosal, injection or the like. Examples of the administration form include oral administration or intravenous injection such as tablets, capsules, granules, powders, syrups, etc., intramuscular injections, suppositories, inhalants, percutaneous absorption agents, eye drops, nasal drops, etc. Parenteral administration. In order to prepare pharmaceutical preparations of such various dosage forms, the above-described composition is further added to a pharmaceutically acceptable excipient, binder, extender, disintegrant, lubricant, and dispersant. , Buffering agents, preservatives, flavoring agents, fragrances, coating agents, carriers, diluents, and the like can be added in appropriate combinations. Among the administration forms, the preferred form is oral administration, and the content of the extract of sputum in the preparation when used as a preparation for oral administration is preferably about 0.1 to 70% by mass, for example.

  When used as a medicine, the daily dose per adult varies depending on various factors such as age, weight, sex, and administration method, but in the case of oral administration, the above plant or extract thereof (in terms of solid content) ), For example, 0.1 to 5000 mg is preferably administered once a day to several times a day.

  In the case of using the composition as a food, the same form (tablet, capsule, syrup, etc.) as the above-mentioned oral administration preparation can be mentioned. The composition may be appropriately blended in various beverages, snacks, dairy products, seasonings, processed starch products, processed meat products, and the like.

  In order to prepare various forms of food products, the above-mentioned extract or composition is further added to other food materials, solvents, softeners, oils, emulsifiers, preservatives, fragrances, stabilizers, colorants, ultraviolet rays. Absorbers, antioxidants, humectants, thickeners, and the like can be added in appropriate combinations. In order to efficiently use the active ingredient in the koji, it is easy to use industrially and it is preferable to add the additive to the extract as a form that does not impair the flavor of the food. In general, the content of the koji extract in the food product is preferably about 0.002 to 5% by mass, for example.

  In the present invention, a composition comprising an extract obtained by treating salmon with an alcohol such as ethanol as an active ingredient has an effect of suppressing an increase in blood glucose level and an effect of suppressing an increase in blood pressure. That is, the composition can be used as a blood glucose level increase inhibitor or a blood pressure increase suppressant, which contains a salmon alcohol extract as an active ingredient.

  The inhibitory action on the increase in blood glucose level is achieved, for example, by having an α-glucosidase inhibitory activity that inhibits the action of α-glucosidase inhibitory activity in the intestine and inhibits the decomposition and absorption of sugar. α-Glucosidase (AGH) breaks down disaccharides such as maltose produced by breaking down starch into glucose. When this glucose enters the blood, the blood sugar level rises. Therefore, it is considered that inhibiting AGH can reduce the amount of glucose produced and suppress an increase in blood glucose level. The α-glucosidase inhibitory activity can be evaluated using, for example, a rat small intestine-derived enzyme.

  The blood pressure increase inhibitory effect is achieved, for example, by having an angiotensin converting enzyme inhibitory activity that inhibits angiotensin converting enzyme (ACE) involved in increasing blood pressure. An increase in blood pressure involves a pressor mechanism (renin / angiotensin system) and an antihypertensive mechanism (kinin / kallikrein system), and angiotensin converting enzyme is an enzyme that acts on both systems. Angiotensin I is converted to angiotensin II having a vasoconstrictive action by an angiotensin converting enzyme. Furthermore, the enzyme also has an action of inactivating bradykinin that lowers blood pressure, and the action of these two systems is involved in the increase of blood pressure. Therefore, it is considered that an increase in blood pressure can be suppressed by inhibiting angiotensin converting enzyme. The angiotensin converting enzyme inhibitory activity can be detected by enzymatic method of 3-Hydroxybutyric acid (3HB) excised from 3-Hydroxybutyryl-Gly-Gly-Gly (3HB-GGG).

Moreover, the composition mentioned above has anti-obesity action and antioxidant activity.
The anti-obesity effect is achieved by having β-lipase inhibitory activity that inhibits the action of β-lipase in the intestine and inhibits the decomposition and absorption of fat. Antioxidant activity has the activity of diminishing or eliminating harmful reactions involving oxygen.

[Example 1]
The fruit and mature fruits of Fuyu and Jiro are collected at different times, and immediately after collection, they are divided into pericarp, fruits, seeds, and cocoon parts and stored at -80 ° C. As a comparative example, a Chinese herbal medicine “柿蔕 (City, Made in China)” (hereinafter referred to as “Chinese herbal medicine”) was purchased commercially (available from Nakaya Hikojuro Pharmacy).

  In the fruit and mature fruit of Tomiyu and Jiro, 3.0 g of each was brought into contact with 30 mL of 100% ethanol and extracted at 4 ° C. for 3 days (extraction process). The extract was dried under reduced pressure at 45 to 50 ° C. for 180 minutes, and then the obtained extract was redissolved in 100% ethanol so as to be 10% by weight (ethanol fraction) to obtain a sample solution. At this time, precipitation of components insoluble in ethanol and soluble in water was observed. Therefore, after recovering the ethanol-soluble component (ethanol fraction), the remaining ethanol-insoluble component was redissolved in water so as to be 10% by weight (water fraction). The same treatment was performed on the skin, fruit, and seed to prepare a sample solution.

Chinese herbal medicine was extracted with 100% ethanol, hot water, water, and ethyl acetate. Extraction with 100% ethanol and ethyl acetate was performed by the method according to the above-mentioned ethanol of Tomiyu and Jiro.
About hot water extraction, hot water extraction was performed according to prescription. That is, water was added so that it might become a ratio of 100 mL with respect to 1.0 g of soot, and it was made to contact hot water of 100 degreeC, and hot water extraction was performed. After extraction, it was dried under reduced pressure under the above-mentioned conditions, and redissolved in water to 10% by weight.
About water extraction, it performed by the method according to this hot water extraction.

Thin layer chromatography (TLC) analysis was performed on the persimmons, pericarps, seeds, and fruits (ethanol extraction stock solution) of Fuyu persimmon fruit and Jiro persimmon fruit.
The sample solution was charged on a TLC plate (20 cm × 20 cm, Kieselgel 60 F254, Merck) and then developed for 2 hours (developing solvent: chloroform-ethyl acetate-formic acid = 5: 4: 1). After completion of the development, the detection reagent was sprayed and the plate was heated at 110 ° C. As the detection reagent, p-anisaldehyde targeting all organic compounds and DPPH (1,1-diphenyl-2-picrylhydrazyl) for detecting antioxidant activity were used. After the color development, the colored spots were observed. The results are shown in FIG.

  When p-anisaldehyde was used as a detection reagent (left side of FIG. 1), the number and color of spots appearing on the persimmon, pericarp, fruit, and seed differed in the Fuyu persimmon fruit and the Jiro persimmon fruit. As a result, it was recognized that the components contained were different for each of cocoons, pericarps, fruits and seeds.

  In Chinese herbal medicine, the number and color of spots appearing in the samples extracted by ethanol, water, hot water extraction, and ethyl acetate extraction were different. As a result, it was recognized that the components recovered by the extraction method were different in traditional Chinese medicine.

When DPPH was used as a detection reagent (right side of FIG. 1), spots and intensity differences showing antioxidant activity were confirmed in Fuyu persimmon fruit, Jiro persimmon fruit and Chinese herbal medicine. In addition, in the Fuyu persimmon fruit and the Jiro persimmon fruit, the spots and strength differences showing antioxidant activity were also confirmed in each of the persimmon, pericarp, fruit and seed.
In the case of Kampo medicine, the detection of antioxidant activity using DPPH did not detect compounds with antioxidant activity in the ethyl acetate extract, so the compounds involved in antioxidant activity are mainly hydrophilic compounds. It turns out that there is.

[Example 2]
The following experiment was conducted using the sample solution of the buds of Tominari and Jiroro prepared in Example 1 (young fruit, mature fruit). The Chinese herbal medicine used the sample solution which extracted hot water.

  Each sample (ethanol extraction stock solution) was subjected to thin layer chromatography (TLC) analysis. The results are shown in FIG.

When p-anisaldehyde is used as a detection reagent (left side of FIG. 2), Tomi Yu (mature fruit: experimental example 1-1, young fruit: experimental example 2-1), Jiro (mature fruit: experimental example 3-1) , Young Fruit: In Experimental Example 4-1) and Traditional Chinese Medicine (Comparative Example 1-1), the number and color of spots appearing in each were different. As a result, it was recognized that the components contained in varieties differ depending on the variety.
In addition, there was a difference in the number and color of spots in the comparison of mature fruits and young fruits of Togashi and Jiro. From this, it was recognized that the components contained in the koji differ depending on the maturity of the koji.

  When DPPH was used as a detection reagent (right side of FIG. 2), Togashi Yu (mature fruit: Experimental Example 1-2, young fruit: Experimental Example 2-2) Results: Many spots showing antioxidant activity were confirmed as compared with Experimental Example 4-2) and Chinese herbal medicine (Comparative Example 1-2). In addition, a difference in color intensity was confirmed between mature fruits (Experimental Example 1-2) and young fruits (Experimental Example 2-2) in Fuyu persimmon. Thereby, it was recognized that antioxidant activity changes with varieties and maturity.

Example 3
The α-glucosidase inhibitory activity (inhibition of blood sugar level increase) was investigated for the ethanol fraction and water fraction of Fuyu persimmon (young fruit, mature fruit), Jiro persimmon (young fruit), and Chinese herbal medicine prepared in Example 1. It was.

  The enzyme solution was prepared as follows. That is, 1.0 g rat intestinal acetone powder was suspended in 10 mL of 0.1 M sodium phosphate buffer (pH 7.0) and stirred for 30 minutes under ice cooling. Thereafter, centrifugation was performed (12000 rpm × 10 minutes, 4 ° C.), and the supernatant was used as a crude enzyme solution. The prepared enzyme solution was stored at -80 ° C.

0.4 mL of 250 mM maltose aqueous solution was added as a substrate to 0.48 mL of distilled water. To this reaction solution, 0.1 mL of each extract (ethanol fraction and water fraction, final concentration: 1 to 20 mg / mL) was added as an inhibitor, followed by preincubation at 37 ° C. for 5 minutes after stirring. Thereafter, 0.02 mL of α-glucosidase crude enzyme solution was added, and the reaction was performed at 37 ° C. for 40 minutes. After completion of the reaction, 1.0 mL of 0.2 M sodium carbonate aqueous solution was added to stop the reaction. Glucose produced in the same reaction was measured using Glucose CII-Test Wako. After determining the residual activity, the 50% inhibitory concentration (IC ₅₀ , mg / ml) was calculated. The results are shown in Table 1 (the smaller the value, the higher the activity).

With regard to Chinese herbal medicine, the activity (IC ₅₀ = 16.7 mg / ml) was confirmed only in the water fraction.
As for the persimmon of Fuyu persimmon (mature fruit), the activity was confirmed in the ethanol fraction (IC ₅₀ = 5.8 mg / mL) and the water fraction (IC ₅₀ = 15.7 mg / mL), and the ethanol fraction was higher. Had activity.
As for the persimmon of Fuyu persimmon (young fruit), the activity (IC ₅₀ = 2.5 mg / mL) was confirmed only in the ethanol fraction, which was higher than the mature fruit.
As for Jiro koji (young fruit) cocoons, activity (IC ₅₀ = 6.6 mg / mL) was confirmed only in the ethanol fraction, which was the second highest activity after Furuyu koji berries.

  Based on the above, it was recognized that the buds of Fuyu persimmon (young fruit) and Jiro persimmon (young fruit) have higher α-glucosidase inhibitory activity than fruits, seeds and pericarp. In addition, it was recognized that the Fuyu persimmon (mature fruit) persimmon has higher α-glucosidase inhibitory activity than the fruit and pericarp.

Example 4
The angiotensin-converting enzyme inhibitory activity (blood pressure increase inhibitory activity) was investigated for the water fractions of the fragrance of Fuyu and Jiroro prepared in Example 1 and the water fractions of Chinese medicine pods.
In this test, the inhibitory activity against angiotensin converting enzyme involved in blood pressure increase was measured using Acekit-Double Estee (Angiotensin converting enzyme inhibitory activity measuring kit) (Dojindo Laboratories Co., Ltd.). ₅₀ ). Since the kit has restrictions on the organic solvent used for dissolving the sample and its concentration in measuring the activity, only the water fraction was examined for inhibitory activity.

  The test was conducted according to the method of using the kit. That is, 20 μL of an angiotensin converting enzyme and aminoacylase mixed solution prepared in ultrapure water, 20 μL of a substrate solution (3-Hydroxybutyryl-Gly-Gly-Gly (3HB-GGG)), and 20 μL of a sample solution are mixed and incubated at 37 ° C. for 1 hour. Reacted. To this, 200 μL of a mixture of 3-hydroxybutyrate dehydrogenase and coenzyme was added and allowed to react at room temperature for 10 minutes. After the reaction, absorbance at 450 nm was measured, and the inhibition rate was calculated based on the obtained data. The results are shown in Table 2 (smaller values indicate higher activity).

  As a result, although there was a difference in activity depending on the cultivar, inhibitory activity against angiotensin converting enzyme was observed in any pod. The highest activity was seen in Fuyu-an (mature fruit).

Example 5
Β-lipase inhibitory activity (anti-obesity action) was examined for the ethanol fraction and water fraction of Togashi Yuji, Jiro, and Kampo medicines prepared in Example 1.

To 50 μL of the fluorescent methylumbelliferone oleate solution, 25 μL of the sample solution (final concentration: 2.5 to 2500 mg / mL) was added and preincubated at 37 ° C. for 5 minutes. Thereafter, 25 μL of β-lipase was added, and an enzyme reaction was performed at 37 ° C. for 30 minutes. After completion of the reaction, 50 μL of 100 mM citrate buffer (pH 4.2) was added to stop the reaction, and the fluorescence of released 4-methylumbelliferone was measured at an excitation wavelength of 355 nm and a fluorescence wavelength of 460 nm. After determining the residual activity, the 50% inhibitory concentration (IC ₅₀ , μg / mL) was calculated. The results are shown in Table 3 (smaller values indicate higher activity).

With regard to Chinese herbal medicine, the activity was confirmed in both fractions, and the water fraction (IC ₅₀ = 7.2 μg / mL) had higher inhibitory activity.
In the case of Fuyu persimmon (mature fruit), activity was observed in both fractions, but the water fraction (IC ₅₀ = 5.7 μg / mL) had higher inhibitory activity.
As for the persimmon of Fuyu persimmon, the activity was observed in both fractions, but the water fraction (IC ₅₀ = 23.1 μg / mL) had higher inhibitory activity.
As for Jiro koji (mature fruit) koji, activity was observed in both fractions, but the water fraction (IC ₅₀ = 1.7 μg / mL) had high inhibitory activity, and the highest activity among those tested. Met.
As for Jiro koji (young fruit) koji, activity was observed in both fractions, and the water fraction (IC ₅₀ = 6.9 μg / mL) had higher inhibitory activity.

  Based on the above, it was recognized that the buds of Fuyu and Jiro have higher β-lipase inhibitory activity than any part of the fruit skin, fruit or seed.

Example 6
With respect to the ethanol extraction stock solutions of Togashi Rin, Jiro, and Chinese herb prepared in Example 1, the amount of polyphenol and the amount of antioxidant activity were quantified.

  The amount of polyphenol was measured using the foreign thiocult method. 0.45 mL of 50% foreign thiocult reagent was added to 0.09 mL of each extract and stirred. After 3 minutes, 0.45 mL of 0.4 M sodium carbonate aqueous solution was added, stirred, and incubated at 55 ° C. for 5 minutes. The reaction solution was allowed to cool for 1 hour to remove bubbles, and the absorbance at 765 nm was measured. At this time, the amount of polyphenol was calculated from a gallic acid equivalent (mg-gallic acid equivalent / 1.0 g) per 1.0 g of sample fresh weight by preparing a calibration curve using gallic acid.

  Antioxidant activity was measured under light-shielding conditions using a brown test tube. To 1.0 mL of 100 mM Tris-HCl buffer (pH 7.4), 2.0 mL of 0.5 M DPPH ethanol solution was added and stirred, and 1.0 mL of the sample solution was added. After incubation at 24 ° C. for 20 minutes, the absorbance at 525 nm was measured. At this time, the antioxidant activity was calculated using a Trolox calibration curve and the equivalent of Trolox per μg of fresh sample weight (μmol-trolox equivalent / 1.0 g).

  The results are shown in Table 4.

  In the cocoon region, both the amount of polyphenol (7.25 mg-gallic acid equivalent / 1.0 mg) and antioxidant activity (10.09 μmol-trolox equivalent / 1.0 mg) have the highest value for Fuyu (fruit). Indicated. In addition, for seeds, fruits and pericarp, Fuyu persimmon (young fruit) seeds (polyphenol content: 20.47 mg, antioxidant activity: 12.42 μmol) and Jiro persimmon (young fruit) seeds (polyphenol content: 13.91 mg, antioxidant activity) : 6.17 μmol) showed a high polyphenol content and an antioxidant activity value. Chinese herbal medicines had lower polyphenol content and antioxidant activity than Fuyu and Jiro.

Example 7
The ethanol extract of Fuyu candy, Jiro candy (young fruit, mature fruit) and Chinese medicine candy prepared in Example 1 was dried under reduced pressure and subjected to GC-MS analysis.

In the GC-MS analysis, each sample was subjected to trimethylsilyl derivatization (TMS derivatization). For GC-MS analysis, Agilent 6890N (manufactured by Agilent Technologies) is used as the GC apparatus, and J & W DB-5 (manufactured by Agilent Technologies, 30 m × 0.25 mm-id, film thickness of 0.25 μm) is used as the column. And an Agilent 5972 (manufactured by Agilent Technologies) was used as the MS detector.
In addition, ursolic acid, oleanolic acid, betulinic acid, and β-sitosterol were similarly derivatized with TMS, and the retention time and fragment ion peak were compared. The results are shown in FIG.

In GC-MS analysis, no significant component difference (difference in peak) was found between Tomiyu and Jiro.
Comparing Fuyu-an, Jiro-an and Chinese herbal medicines, it was confirmed that in the retention time of 20.000 to 25.000 minutes, the ingredients in Fuyu-an, Jiro-an, but not in Kampo medicines were found. In addition, a peak that was not found in the buds of Fuyu and Jiro at 8.598 minutes was confirmed in the Kampo medicine basket. Thus, in GC-MS, the difference of the contained component in Fuyu and Jiro was and Chinese herbal medicine was shown.
In any sputum, triterpenoids (retention time, ursolic acid: 29.683 minutes, oleanolic acid: 29.206 minutes, betulinic acid: 29.326 minutes) and β-sitosterol (27.256 minutes) were detected. It was done.

  The present invention can be used for a method for extracting sputum, and the extracted composition can be used as a pharmaceutical and a food having a blood glucose level increase suppressing action and a blood pressure increase suppressing action.

Claims (3)

  A blood glucose level increase inhibitor comprising an alcoholic extract of persimmon as an active ingredient.   An antihypertensive agent comprising an alcoholic extract of salmon as an active ingredient.   A method for extracting koji, which comprises an extraction step of bringing koji into contact with alcohol to extract active ingredients.