JP2015146736A - Multifaceted microorganism culture sheet and use method thereof - Google Patents

Abstract

PROBLEM TO BE SOLVED: To easily and reliably conduct the measurement of a colony and the addition of a sample solution.SOLUTION: A multifaceted microorganism culture sheet 20 comprises a single substrate sheet 11, a plurality of frame bodies 14 provided on the substrate sheet, and culture media 21 in the frame bodies 14. Each of the culture media 21 is covered with an independent cover sheet 13.

Description

  The present invention relates to a multifaceted microorganism culture sheet and a method of using the same, and more particularly to a multifaceted microorganism culture sheet and a method of using the same that can easily perform colony counting and sample solution addition.

  Many microorganism culture sheets for measuring microorganisms in food or the environment have been devised. A typical microorganism culture sheet is composed of a rectangular waterproof sheet, a frame formed thereon, a medium formed inside the frame, and a cover sheet covering the medium. In recent years, the microorganism culture sheet has been widely used as an alternative to the agar medium because it has advantages such as less time for medium adjustment, less space, and reduced disposal costs compared to the conventional agar medium.

   For example, a cold water-soluble powder containing a water-based adhesive composition layer and a gelling agent attached to the adhesive composition layer is formed on the surface of the waterproof sheet, and the cold water-soluble powder is covered with the cover sheet. A microorganism culture sheet is obtained by covering with. In such a microorganism culture sheet, the cover sheet is peeled off, and the sample solution is added onto the cold water soluble powder layer. In this case, the gelling agent quickly hydrates and forms a reconstitution medium that maintains microbial growth. Next, when the cover sheet is covered and incubated for a predetermined time, colonies can be counted through the transparent cover sheet.

Japanese Examined Patent Publication No. 2-49705 International Publication No. W001 / 044437 JP-T-2004-515236 JP-A-8-280377

  In any microorganism culture sheet, it is necessary to add a sample solution for each microorganism culture sheet. In addition, it is necessary to measure each colony culture sheet for each microorganism culture sheet. Is on.

  The present invention has been made in consideration of such points, and provides a multifaceted microorganism culture sheet that can easily perform colony measurement and can reliably add a sample solution, and a method of using the same. The purpose is to do.

  The present invention includes a base sheet, a plurality of medium forming areas provided on the base sheet, a medium arranged in each medium forming area and configured to culture colonies of microorganisms, A plurality of cover sheets that independently cover the culture medium, each cover sheet having one side edge fixed to the base sheet, and holding the other side edge to open the cover sheet. It is a sheet.

  In the present invention, a printing unit including management information on the culture medium may be provided on the base sheet corresponding to each culture medium.

  In the present invention, cuts for partitioning each medium formation region may be formed on the substrate sheet.

  In the present invention, different types of culture media may be disposed on the base sheet.

  In the present invention, the color of the cover sheet covering one type of medium may be different from the color of the cover sheet covering another type of medium.

  In the present invention, each cover sheet provided on the base sheet may have an opening direction that falls within an angle range of 45 °.

  The present invention includes a base sheet, a plurality of frames provided on the base sheet, a medium arranged in each frame and configured to culture colonies of microorganisms, and each medium independently. Each of the cover sheets has a side edge fixed to the base sheet, and the cover sheet is opened by gripping the other side edge to cover the culture medium. A multifaceted microorganism culture sheet comprising: a step of holding the other side edge of the cover sheet to open the cover sheet; and a step of adding a sample solution onto the medium in which the cover sheet is opened. How to use.

  As described above, according to the present invention, colony measurement and sample solution addition can be performed easily and reliably.

FIG. 1 is a plan view showing a multi-sided microorganism culture sheet according to the first embodiment of the present invention. FIG. 2 is a perspective view showing a multifaceted microorganism culture sheet. FIG. 3 is a plan view showing a multi-faceted microorganism culture sheet after a sample solution is attached. FIG. 4 is a view showing a state in which a multi-sided microorganism culture sheet is inspected by a scanner. FIG. 5 is a side view showing a multi-sided microorganism culture sheet. 6 (a) and 6 (b) are plan views showing a multi-sided microorganism culture sheet according to the second embodiment of the present invention. FIG. 7 is a plan view showing a multi-imposition microorganism culture sheet according to the third embodiment of the present invention. FIG. 8 is a plan view showing a multi-imposition microorganism culture sheet according to the fourth embodiment of the present invention. 9 (a) and 9 (b) are plan views showing a multifaceted microorganism culture sheet according to the fifth embodiment of the present invention. FIG. 10 is a plan view showing a multi-imposition microorganism culture sheet according to the sixth embodiment of the present invention. FIG. 11 is a view showing the opening direction of the cover sheet of the multi-faced microorganism culture sheet.

Embodiment of the Invention

First Embodiment Hereinafter, a first embodiment of the present invention will be described with reference to the drawings.
1 to 5 are views showing a first embodiment of the present invention.

  As shown in FIG. 1 to FIG. 5, a multifaceted microorganism culture sheet 20 includes a single rectangular waterproof sheet (base sheet) 11 and a plurality of, for example, eight circular frames provided on the base sheet 11. And a body 14. Here, the single base sheet refers to a single base sheet. In addition, each frame 14 provided on the base sheet 11 defines a medium formation region. A medium configured to culture colonies of microorganisms is arranged in each medium forming area, and a medium area 21 (also referred to as a medium) described later is formed. Moreover, the recessed part which functions as a culture medium formation area | region directly in the board | substrate sheet | seat 11 without providing the frame 14 may be formed, and a culture medium may be formed in each recessed part.

  Moreover, the culture medium area | region 21 comprised so that the colony 21a was culture | cultivated was formed in each frame 14 provided on the base material sheet 11, and the outer side of the frame body 14 is a non-medium area | region (it is also called parts other than a culture medium). 22 Further, the base sheet 11 is provided with a cover sheet 13 that independently covers the culture medium 21 provided in each frame 14.

  Moreover, the cover sheet 13 covering the culture medium 21 in each frame 14 is fixed to the base sheet 11 at one side edge 13a, and the other side edge 13b of the cover sheet 11 is not fixed to the base sheet 11. The cover sheet 11 can be opened by gripping and lifting the other side edge 13 b of each cover sheet 11.

  By the way, such a multifaceted microorganism culture sheet 20 is produced by the following method. That is, first, a rectangular waterproof sheet (base sheet) 11 is prepared, and a plurality of, for example, eight frame bodies 14 are formed on the base sheet 11. Next, a medium 21 having a dry medium layer containing a gelling agent, a nutrient, a water absorbing agent, an indicator, and the like is formed in each frame body 14.

  Next, on the base material sheet 11, a cover sheet 13 that covers the culture medium 21 in each frame 14 is provided independently for each culture medium 21. In this case, each cover sheet 13 is heat-sealed and fixed on the base sheet 11 at one side edge 13a, and the other side edge 13b is not fixed to the base sheet 11 and is easily grasped and lifted. Can do.

  As shown in FIG. 1, in the multi-sided microorganism culture sheet 20, the cover sheet 13 covering each medium 21 has one side edge 13 a located on the left side, so that the cover sheet 13 is opened from the right side to the left side. They are in the same direction (see FIG. 1).

  In this case, as shown in FIG. 11, the opening direction of each cover sheet 13 may be within an angle range α of 45 ° from the right side to the left side, preferably within the angle range α of 15 °, more preferably. Is within an angular range α of 5 °. More preferably, the opening direction of each cover sheet 13 is the same direction from the right side to the left side as described above.

  Here, the angle range α that is the opening direction of the cover sheet 13 extends symmetrically about the conveyance direction L of the multifaceted microorganism culture sheet 20.

  In this way, the opening direction of each cover sheet 13 is set to an angle range α of 45 ° from the right side to the left side, preferably an angle range α of 15 °, more preferably an angle range α of 5 °, and further preferably the same direction. Thus, the operator can easily lift each cover sheet 13 from the same position. Further, when the multi-faceted microorganism culture sheet 20 is put into the scanner 30 described later, the cover sheet 13 can be passed through the conveyance path without being rolled. Next, the material of each member of the multifaceted microorganism culture sheet 20 will be described. The single base sheet 11 and the cover sheet 13 are both made of a transparent synthetic resin, and the frame body 14 is made of a hydrophobic synthetic resin. Moreover, the culture medium 21 consists of a dry culture medium layer containing a gelatinizer, a nutrient, a water absorbing agent, etc. as mentioned above.

Next, the operation of the present embodiment having such a configuration will be described.
First, a multifaceted microorganism culture sheet in which eight frame bodies 14 and a culture medium 21 are formed on the substrate sheet 11 described above is prepared (see FIG. 2).

  Next, a sample solution containing microorganisms is added (inoculated) on the medium 21 in each frame 14. In this case, first, the other side edge 13b of the cover sheet 13 covering the culture medium 21 is gripped, and the cover sheet 13 is lifted. This opens the cover sheet 13 and exposes the culture medium 21. Next, a sample solution can be added onto the exposed medium 21.

  By adding the sample solution onto the medium 21, gelation of the medium proceeds by the gelling agent, and the microorganisms in the gelled medium 21 can be cultured under appropriate conditions. As a result, colonies 21a are formed in the culture medium 21 (see FIG. 3). In this case, the colony 21a is colored by the indicator in the gelled medium 21.

  After adding the sample solution on one medium 21 in this way, the sample solution is sequentially added on the next medium 21 in the same manner, and thus the sample is added to all the mediums 21 on the base sheet 11. Liquid can be added.

  Thereafter, the multi-sided culture film 20 as a whole is placed in an incubator and cultured for a predetermined time, whereby colonies 21a can be formed in each culture medium 21.

  Thereafter, as shown in FIG. 4, the multi-sided microorganism culture sheet 20 is put into the scanner 30 as a whole, and the number of colonies 21 a formed in each medium 21 of the multi-sided microorganism culture sheet 20 is measured by the scanner 30. Can do.

  As described above, according to the present embodiment, the multi-imposition microorganism culture sheet 20 has a plurality of (eight) culture media 21 provided on the single base sheet 11, and thus has a single culture medium. Compared with the case where the microorganism culture sheets are put into the scanner 30 one by one, the multi-sided microorganism culture sheet 20 is fed into the scanner 30 as a whole, so that it can be easily and quickly formed on each medium 21 using the scanner 30. Colonies 21a can be measured.

  Moreover, since each culture medium 21 of the multi-sided microorganism culture sheet 20 is covered with an independent cover sheet 13, one cover sheet 13 is opened to expose the corresponding culture medium 21, and a sample is placed on the exposed culture medium 21. When adding the liquid, the other medium 21 can be covered with the corresponding cover sheet 13. For this reason, when a sample solution is added onto one medium 21, the sample solution is not mistakenly added onto another medium 21, and other medium 21 is inadvertently exposed and dust or the like is left. There is no contamination.

  Furthermore, since the opening directions of the cover sheets 13 covering the respective culture media 21 of the multifaceted microorganism culture sheet 20 are the same, the operation of opening the cover sheets 13 can be performed quickly.

Second Embodiment Next, a second embodiment of the present invention will be described with reference to FIGS.
In the second embodiment shown in FIGS. 6A and 6B, one side edge 13 a fixed to the base sheet 11 is provided adjacent to each other among a pair of adjacent cover sheets 13. . Other configurations are substantially the same as those of the first embodiment shown in FIGS.

  In the second embodiment shown in FIGS. 6A and 6B, the same parts as those in the first embodiment shown in FIGS.

  In FIG. 6A, four frame bodies 14 are provided on a single base sheet 11, and an independent cover sheet 13 is provided so as to cover the culture medium 21 installed in each frame body 14. .

  Of the pair of upper cover sheets 13, one side edge 13 a fixed to the base sheet 11 is adjacent to each other, and similarly, one side edge of the lower pair of cover sheets 13 fixed to the base sheet 11. 13a are also adjacent to each other.

  Furthermore, in FIG.6 (b), a pair of frame body 14 is provided on the single base material sheet 11, and the independent cover sheet 13 which covers the culture medium 21 installed in each frame body 14 is provided. . Moreover, the one side edge 13a fixed to the base material sheet 11 among a pair of cover sheets 13 is adjacent to each other.

  As shown in FIGS. 6A and 6B, each of the pair of adjacent cover sheets 13 is made adjacent to each other by adjoining one side edge 13 a fixed to the base sheet 11 among the pair of adjacent cover sheets 13. The one side edge 13a can be fixed on the base sheet 11 by heat sealing at the same time.

Third Embodiment Next, a third embodiment of the present invention will be described with reference to FIG.
In the third embodiment shown in FIG. 7, a cut 15 surrounding each frame 14 is formed on a single base sheet 11. Other configurations are substantially the same as those of the first embodiment shown in FIGS.

  In the third embodiment shown in FIG. 7, the same parts as those in the first embodiment shown in FIG. 1 to FIG.

  In FIG. 7, eight frame bodies 14 are provided on a single base sheet 11 in two stages × 4 rows, and cover sheets 13 that are independent and cover the culture medium 21 installed in each frame body 14. Is provided.

  In addition, the base sheet 11 is provided with cuts 15 that divide the region of the frame body 14 provided over 2 rows × 4 rows.

  In FIG. 7, the base sheet 11 is broken along the cuts 15 formed in the base sheet 11, for example, so that the base sheet 11 has two base sheets 11 having a frame 14 in one stage × 4 rows. Or it can also divide | segment into the two base material sheets 11 which have the frame 14 of 2 steps | paragraphs x 2 rows.

Fourth Embodiment Next, a fourth embodiment of the present invention will be described with reference to FIG.
In the fourth embodiment shown in FIG. 8, a printing unit 16 including management information of the frame body 14 is provided on the base sheet 11 adjacent to each frame body 14. Other configurations are substantially the same as those of the first embodiment shown in FIGS.

  In the fourth embodiment shown in FIG. 8, the same parts as those in the first embodiment shown in FIGS. 1 to 5 are denoted by the same reference numerals, and detailed description thereof is omitted.

  In FIG. 8, four frame bodies 14 are provided on a single base sheet 11, and an independent cover sheet 13 is provided so as to cover the culture medium 21 installed in each frame body 14.

  Further, on the base sheet 11, a printing unit 16 including management information of the frame body 14 is provided adjacent to the frame body 14.

  As such a printing unit 16, a number or a bar code including ID information corresponding to each frame 14 is conceivable, or a QR code (registered trademark) including other management information is conceivable.

Among these, the following information can be incorporated in the QR code (registered trademark).
(Information on microorganism culture sheet)
・ Product ID
・ Imposition position in the sheet ・ Manufacturing factory ・ Production line ・ Manufacturing date ・ Expiration date
・ Inspection ID
・ Inspection time (start / end)
・ Sampling place (collection process)
・ Inspection target (product name / lot name)
・ Person in charge (employee number / name)
・ Test type (generic bacteria, E. coli, etc.)
・ Culture conditions (dilution ratio, type of diluent, temperature, humidity, incubation time)
・ Culture location (incubator number, number of plates, etc.)
According to this Embodiment, the culture medium 21 arrange | positioned in each frame 14 on the base material sheet 11 can be managed separately easily and reliably.

Fifth Embodiment Next, a fifth embodiment of the present invention will be described with reference to FIGS.
In the fifth embodiment shown in FIGS. 9A and 9B, different types of culture media 21A, 21B, and 21C are provided on the base sheet 11, and the culture media 21A, 21B, and 21C are covered with different colors. It is covered with 13A, 13B, 13C. Other configurations are substantially the same as those of the first embodiment shown in FIGS.

  In the fifth embodiment shown in FIGS. 9A and 9B, the same parts as those in the first embodiment shown in FIGS. 1 to 5 are denoted by the same reference numerals, and detailed description thereof is omitted.

  In FIG. 9A, four frame bodies 14 are provided on a single base sheet 11, and different types of culture media 21 </ b> A and 21 </ b> B are provided in each frame body 14. In addition, independent cover sheets 13A and 13B are provided to cover the culture media 21A and 21B.

  As shown in FIG. 9A, a pair of culture media 21A and 21A are provided above the base sheet 11, and a pair of culture media 21B and 21B of a different type from the culture media 21A and 21A are provided below. Yes.

  The color (for example, yellow) of the cover sheet 13A covering the upper pair of culture media 21A and 21A is different from the color (for example, green) of the cover sheet 13B covering the lower pair of culture media 21B and 21B.

  9B, three frame bodies 14 are provided on a single base sheet 11, and different types of culture media 21A, 21B, and 21C are provided in each frame body 14, and each culture medium 21A, Independent cover sheets 13A, 13B, and 13C are provided to cover 21B and 21C. In FIG. 9B, the color of the cover sheet 13A covering the medium 21A is yellow, the color of the cover sheet 13B covering the medium 21B is green, and the color of the cover sheet 13C covering the medium 21C is purple. It has become.

  In FIG. 9 (a), for example, when two types of sample liquids are tested using two types of culture medium 21, one type of sample liquid is arranged in the vertical direction on the base sheet 11, and the medium 21A and the medium Add to 21B to form colony 21a. Next, another type of sample solution is added to the remaining medium 21A and medium 21B to form colonies 21a.

  In this case, since the cover sheets 13A and 13B of different colors are provided for the different culture media 21A and 21B, the culture media 21A and 21B to be used can be easily and reliably found. There is no addition on the media 21A, 21B.

  Further, in FIG. 9B, for example, when the sample liquid is inspected using three types of medium 21, the sample liquid is added to the medium 21A, medium 21B, and medium 21C arranged in the vertical direction on the base sheet 11. And colonies 21a are formed.

  In this case, since the cover sheets 13A, 13B, and 13C of different colors are provided for the different culture media 21A, 21B, and 21C, the culture media 21A, 21B, and 21C to be used can be easily and reliably found, The liquid is not mistakenly added to the other culture media 21A, 21B, and 21C.

  The color of the colored cover sheets 13A, 13B, and 13C is selected so as not to interfere with this measurement function when the number of colonies 21a is measured by inserting the multifaceted microorganism culture sheet 20 into the scanner 30. .

Sixth Embodiment Hereinafter, a sixth embodiment of the present invention will be described with reference to FIG.
In the sixth embodiment shown in FIG. 10, different types of culture media 21A, 21B, and 21C are provided on the base sheet 11, and the culture media 21A, 21B, and 21C are covered with cover sheets 13A, 13B, and 13C of different colors. It's covered. Other configurations are substantially the same as those of the fifth embodiment shown in FIGS.

  In the sixth embodiment shown in FIG. 10, the same parts as those in the fifth embodiment shown in FIGS. 9A and 9B are denoted by the same reference numerals, and detailed description thereof is omitted.

  In FIG. 10, twelve frame bodies 14 are provided on a single base sheet 11, and different types of culture media 21 </ b> A, 21 </ b> B, and 21 </ b> C are provided in each frame body 14. In addition, independent cover sheets 13A, 13B, and 13C are provided to cover the culture media 21A, 21B, and 21C.

  As shown in FIG. 10, four culture media 21A, 21A, 21A, 21A are provided in the upper stage of the base sheet 11, and four culture media of a different type from the culture media 21A, 21A, 21A, 21A are provided in the middle stage. 21B is provided. In the lower stage, four culture media 21C different from both the culture media 21A and the culture media 21B are provided.

  The color of the cover sheet 13A covering the upper culture medium 21A is yellow, the color of the cover sheet 13B covering the middle culture medium 21B is green, and the color of the cover sheet 13C covering the lower culture medium 21C is purple. It has become.

  In addition, the base sheet 11 is formed with cuts 15 that partition the regions of the frame bodies 14.

  In FIG. 10, for example, when testing is performed on four types of sample solutions using three types of culture media 21, the first type of sample solution is arranged on the base sheet 11 in the right side of the culture medium 21 </ b> A. And added to the culture medium 21B and the culture medium 21C to form a colony 21a. Next, a second type of sample solution is added to the second stage medium 21A, medium 21B, and medium 21C from the right to form colonies 21b. Similarly, the third type sample solution and the fourth type sample solution are added to the remaining medium 21A, medium 21B, and medium 21C to form colonies 21b.

  In this case, since the cover sheets 13A, 13B, and 13C of different colors are provided for the different culture media 21A, 21B, and 21C, the culture media 21A, 21B, and 21C to be used can be easily and reliably found, The liquid is not mistakenly added to the other culture media 21A, 21B, and 21C.

  In FIG. 10, the base sheet 11 is broken along the cuts 15 formed in the base sheet 11, for example, so that the base sheet 11 has three base sheets 11 having a frame 14 of one step × 4 rows. Alternatively, it can be divided into two base sheet 11 having a frame 14 of 3 stages × 2 rows.

DESCRIPTION OF SYMBOLS 11 Base material sheet 13 Cover sheet 13a One side edge 13b Other side edge 13A, 13B, 13C Cover sheet 14 Frame 15 Cut | interruption 16 Printing part 20 Multi-faced microorganism culture sheet 21 Medium

Claims (7)

A base sheet;
A plurality of medium forming regions provided on the base sheet;
A medium arranged in each medium forming region and configured to culture colonies of microorganisms;
A plurality of cover sheets that individually cover each medium,
Each cover sheet has one side edge fixed to the base material sheet, and holds the other side edge to open the cover sheet.   The multi-sided microorganism culture sheet according to claim 1, wherein a printing unit including management information on the culture medium is provided on the base sheet corresponding to each culture medium.   3. The multifaceted microorganism culture sheet according to claim 1 or 2, wherein a cut line for dividing each medium formation region is formed on the substrate sheet.   The multi-faced microorganism culture sheet according to any one of claims 1 to 3, wherein different types of culture media are arranged on the base sheet.   The multi-faced microorganism culture sheet according to claim 4, wherein the color of the cover sheet covering one type of medium is different from the color of the cover sheet covering another type of medium.   The multi-faced microorganism culture sheet according to claim 1, wherein each cover sheet provided on the base sheet has an opening direction that falls within an angle range of 45 °. A base sheet, a plurality of frames provided on the base sheet, a medium arranged in each frame and configured to culture colonies of microorganisms, and a cover sheet that covers each medium independently In each of the cover sheets, in one side edge is fixed to the base sheet, the other side edge is gripped and the cover sheet is opened to open the cover sheet.
Gripping the other side edge of the cover sheet covering the medium, and opening the cover sheet;
Adding a sample solution onto the medium with the cover sheet open;
A method for using a multi-faceted microorganism culture sheet comprising:

Images