JP2015128381A - Culture device - Google Patents
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- JP2015128381A JP2015128381A JP2014000668A JP2014000668A JP2015128381A JP 2015128381 A JP2015128381 A JP 2015128381A JP 2014000668 A JP2014000668 A JP 2014000668A JP 2014000668 A JP2014000668 A JP 2014000668A JP 2015128381 A JP2015128381 A JP 2015128381A
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- upper lid
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- gasket
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- 229920002379 silicone rubber Polymers 0.000 claims abstract description 20
- 239000004945 silicone rubber Substances 0.000 claims abstract description 20
- 238000012856 packing Methods 0.000 abstract description 30
- 239000000463 material Substances 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000011521 glass Substances 0.000 description 9
- 238000007789 sealing Methods 0.000 description 5
- 229910001220 stainless steel Inorganic materials 0.000 description 5
- 239000010935 stainless steel Substances 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 4
- 210000004102 animal cell Anatomy 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 1
- 229920000178 Acrylic resin Polymers 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- -1 alkalis Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229920003217 poly(methylsilsesquioxane) Polymers 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
本発明は、上蓋と、槽内部品と、上蓋固定装置と、培養槽本体とを含む培養装置に関し、上蓋と培養槽本体との間を気密、液密に密閉するために用いられるガスケット乃至パッキンを必要としない培養装置に関する。 The present invention relates to a culture apparatus including an upper lid, a tank internal part, an upper lid fixing device, and a culture tank main body, and a gasket or packing used for hermetically and liquid-tightly sealing between the upper lid and the culture tank main body. The present invention relates to a culture apparatus that does not need to be used.
従来、培養装置の上蓋と培養槽本体との間を気密、液密に密閉するためにガスケットが用いられていた。この理由は、上蓋や培養槽本体の材質がステンレスや硬い硝子でできているために培養槽本体の内部を気密、液密とするためには、どうしてもガスケット乃至パッキンが必要となるためである。
そして、ガスケット乃至パッキンの材料としてはゴム系の弾力性のある材料が用いられてきた。
Conventionally, a gasket has been used to hermetically and liquid-tightly seal between the upper lid of the culture apparatus and the culture vessel body. This is because the material of the upper lid and the culture tank main body is made of stainless steel or hard glass, so that a gasket or packing is inevitably required to make the inside of the culture tank main body airtight and liquid-tight.
A rubber-based elastic material has been used as a gasket or packing material.
又、培養装置の上蓋には撹拌機が設置され、各種センサー類が多数配置されるため、槽内部品を設置するスペース不足から、槽内部品を上蓋ではなく培養槽本体に貫通孔を設けて設置することが行われている。
この場合、培養槽本体は材質がステンレスやガラス等であるため貫通孔を気密、液密とするためのパッキン部品等が必要であった。
そして、本発明者は下記特許文献１において、培養槽本体ではなく、上記ガスケット乃至パッキンに水平方向の貫通孔を設け、該貫通孔に槽内部品の一部を嵌め込んだ培養装置を発明している。
In addition, since there are agitators on the top lid of the culture device and a large number of various sensors are arranged, because of the lack of space to install the tank parts, the tank parts are not provided with a through hole in the culture tank body. It is done to install.
In this case, since the material of the culture tank main body is stainless steel or glass, packing parts for making the through-hole airtight and liquid-tight are necessary.
And inventor invented the culture apparatus which provided the through-hole of the horizontal direction in the said gasket thru | or packing instead of the culture tank main body in the following patent document 1, and fitted a part of tank internal part in this through-hole. ing.
近年、センサー、冷却コイル、サンプリング管等、多種類の槽内部品を取り付ける必要が生じ、従来の上蓋に十分なスペースが無いという課題が生じている。特に、５リットル程度以下の小型の培養槽においては上蓋のスペース不足は切実な問題であった。
また、上蓋に槽内部品を取り付けると上蓋が重くなり、上蓋の着脱時等に作業者の負担が大きくなるという問題があった。
そこで、本発明者は、上蓋と培養槽本体との間に設置されたガスケットに着目し、この上下方向の厚みを大きくし、培養槽本体ではなく、上記ガスケット乃至パッキン自体に水平方向の貫通孔を１個以上設け、該貫通孔に槽内部品の一部を嵌め込んだ培養装置を考案した。
In recent years, it has been necessary to attach various types of tank components such as sensors, cooling coils, and sampling tubes, and there has been a problem that there is not enough space in the conventional upper lid. In particular, in a small culture tank of about 5 liters or less, lack of space on the upper lid was a serious problem.
In addition, when the parts in the tank are attached to the upper lid, the upper lid becomes heavy, and there is a problem that the burden on the operator increases when the upper lid is attached or detached.
Therefore, the present inventor has paid attention to the gasket installed between the upper lid and the culture tank main body, and increased the thickness in the vertical direction, so that the horizontal through-hole is not formed in the gasket or the packing itself but the culture tank main body. A culture apparatus was devised in which one or more of these were provided and a part of the in-tank part was fitted into the through-hole.
しかし、この装置では、上蓋、ガスケット乃至パッキンと培養槽本体という３部品を必要としていた。そのため培養槽本体へのガスケット乃至パッキンの載置、ガスケット乃至パッキンへの上蓋の載置及び培養槽本体への取付け等の作業を必要とするため、組立てに手間がかかるだけでなく、培養槽が転倒した際には培養槽本体(硝子)の破損による作業者の怪我のおそれが多々あった。
そこで、本発明者は培養槽本体を、ガスケット乃至パッキンと一体に成形することによって、部品点数を減らし、製作工程の簡素化を図ることができないかを種々検討した結果、培養槽本体自体にガスケット乃至パッキンに使用されるシリコーンゴムを用いることを思いついたのである。
However, this apparatus requires three parts: an upper lid, a gasket or packing, and a culture tank body. For this reason, operations such as placing a gasket or packing on the culture tank body, placing an upper lid on the gasket or packing, and attaching to the culture tank body are not only troublesome to assemble, but also the culture tank When falling, there was a risk of injury to workers due to breakage of the culture tank body (glass).
Therefore, as a result of various examinations as to whether the incubator body can be formed integrally with the gasket or packing to reduce the number of parts and simplify the production process, the inventor has attached the incubator body to the incubator body itself. I came up with the idea of using silicone rubber for packing.
上記の課題を解決するために、以下に示すような手段を講じることとした。すなわち、
(１) 本発明は、上蓋と、槽内部品と、上蓋固定装置と、培養槽本体とを含む培養装置において、前記培養槽本体がシリコーンゴムからなり、且つ、
前記培養槽本体には槽内部品を設置するための少なくとも１以上の貫通孔が形成されており、且つ
前記槽内部品が前記貫通孔に嵌め込まれて保持されることを特徴とする培養装置である。
本発明によれば、シリコーンゴムからなる培養槽本体に貫通孔を設けているので、槽内部品の一部、例えば配管をこの孔に通すことにより、槽内部品を培養槽本体内に保持することができる。
(２) 本発明は又、前記シリコーンゴムを透明のシリコーンゴムで形成したことを特徴とする請求項１記載の培養装置である。
培養槽本体を透明のシリコーンゴムで形成したことにより、ガラス製の培養装置本体のように内部監視が極めて容易で、且つガラス製の培養装置本体よりも衝撃耐性に強く、軽量である。又、通常必要とされる上蓋と培養槽本体との間を気密、液密に密閉するために用いられるガスケット乃至パッキンは不要である。更に、培養槽本体においても槽内部品の配管用のガスケット乃至パッキンは不要となる。
上記手段によれば、以下のような作用効果が得られる。
In order to solve the above problems, the following measures are taken. That is,
(1) The present invention provides a culture apparatus including an upper lid, a tank internal part, an upper lid fixing device, and a culture tank body, wherein the culture tank body is made of silicone rubber, and
In the culture apparatus, at least one or more through-holes for installing the in-tank parts are formed in the culture tank body, and the in-tank parts are fitted and held in the through holes. is there.
According to the present invention, since the through-hole is provided in the culture tank body made of silicone rubber, the part in the tank, for example, the piping is passed through this hole to hold the part in the tank in the culture tank body. be able to.
(2) The culture apparatus according to claim 1, wherein the silicone rubber is formed of a transparent silicone rubber.
Since the culture tank body is formed of transparent silicone rubber, internal monitoring is extremely easy like a glass culture apparatus body, and it is stronger and lighter in impact resistance than a glass culture apparatus body. Further, a gasket or packing used for hermetically and liquid-tightly sealing between the upper lid and the culture vessel main body that are usually required is unnecessary. Further, in the culture tank main body, a gasket or packing for piping of the parts in the tank is not required.
According to the above means, the following effects can be obtained.
上蓋と培養槽本体との間を気密、液密に密閉するために従来用いられているガスケット乃至パッキン、又、培養槽本体に設置される配管用のガスケット乃至パッキンが不要となり、部品点数が大きく減り、製造工程が短縮することによって培養装置の製造コストを大きく下げることができる。 Gaskets and packings conventionally used for airtight and liquid-tight sealing between the upper lid and the culture tank main body, and piping gaskets and packings installed in the culture tank main body are no longer required, resulting in a large number of parts. The manufacturing cost of the culture apparatus can be greatly reduced by reducing the manufacturing process.
又、本発明に係る培養装置では、培養槽本体のスペースは広いので、培養槽本体の任意の箇所に貫通孔を設けることができ、より数多くの槽内部品の取り付けが可能となる。
又、槽内部品の交換も容易になる。
又、槽内部品は、動物細胞の培養、植物細胞の培養、微生物の培養など目的とする培養方法に応じて交換する必要があるが、本発明装置では培養目的に応じて槽内部品を培養槽本体に簡単に取付けることもでき、しかも培養槽本体はシリコーンゴムであるため従来のステンレスやガラス等と比べてはるかに軽量であるので迅速で容易な交換作業が可能となる。何よりも、培養槽本体の破損とそれに伴う作業者の怪我を防止するだけでなく、培養後の菌体の環境汚染のおそれを大幅に軽減する効果が期待できる。
Further, in the culture apparatus according to the present invention, since the space of the culture tank main body is wide, a through hole can be provided at an arbitrary position of the culture tank main body, and a larger number of in-tank parts can be attached.
In addition, replacement of the parts in the tank is facilitated.
In addition, it is necessary to replace the parts in the tank according to the intended culture method such as animal cell culture, plant cell culture, microorganism culture, etc., but in the apparatus of the present invention, the parts in the tank are cultured according to the culture purpose. It can be easily attached to the tank body, and since the culture tank body is made of silicone rubber, it is much lighter than conventional stainless steel, glass, etc., so that quick and easy replacement work is possible. Above all, it can be expected not only to prevent breakage of the main body of the culture tank and injury to the operator accompanying it, but also to greatly reduce the risk of environmental contamination of the cells after the culture.
又、本発明に係る培養装置では、培養槽本体に多数の貫通孔を設けることができるので、培養目的毎に異なる構成の槽内部品を取り付けた培養槽本体を用意したり或いは槽内部品の変更により、上蓋の交換を要せず、培養槽本体と一体の槽内部品の交換のみで、目的とする培養に適した培養装置を提供することができる。 Further, in the culture apparatus according to the present invention, since a large number of through holes can be provided in the culture tank body, a culture tank body with a different tank internal configuration for each culture purpose can be prepared or As a result of the change, it is possible to provide a culture apparatus suitable for the intended culture by only exchanging the parts in the tank integral with the culture tank main body without replacing the upper lid.
以下、本発明の一実施形態に係る培養装置を添付図面に基づいて説明する。 Hereinafter, a culture device according to an embodiment of the present invention will be described with reference to the accompanying drawings.
図１に示すように、培養装置１は、上蓋２と、上蓋固定装置３と、槽内部品４と、培養槽本体５とを備える。本発明の培養装置１は、動物細胞の培養、植物細胞の培養、微生物の培養などを目的とする培養装置である。
上蓋２は、培養槽本体５の上部にガスケット乃至パッキンを介することなく載置されている。これは、培養槽本体５の材質がシリコーンゴムであるため、培養槽本体５自体がガスケット乃至パッキンの働きをするためである。
As shown in FIG. 1, the culture apparatus 1 includes an upper lid 2, an upper lid fixing device 3, a tank internal part 4, and a culture tank body 5. The culture apparatus 1 of the present invention is a culture apparatus intended for animal cell culture, plant cell culture, microorganism culture, and the like.
The upper lid 2 is placed on the upper part of the culture tank main body 5 without a gasket or packing. This is because the material of the culture tank body 5 is silicone rubber, so that the culture tank body 5 itself functions as a gasket or packing.
本発明においては、上蓋２と培養槽本体５との間を気密、液密に密閉するために用いられるガスケット乃至パッキンは不要である。又、培養槽本体５においても槽内部品４の配管用のガスケット乃至パッキンは不要である。これらの理由は、これまでの培養装置においては、上蓋２を培養槽本体５に密着させるために両者の間にガスケット乃至パッキンを設置したり、又、培養槽本体５においては槽内部品４の配管部分にガスケット乃至パッキンを設置したりすることがどうしても必要であったが、本発明の培養装置１では、培養槽本体５がそれ自身でガスケット乃至パッキンの働きをするため、あえてガスケット乃至パッキンは採用する必要がないからである。 In the present invention, a gasket or packing used for hermetically and liquid-tightly sealing between the upper lid 2 and the culture tank main body 5 is not necessary. Further, the culture tank body 5 also does not require a gasket or packing for piping of the tank internal part 4. For these reasons, in the conventional culture apparatus, a gasket or packing is installed between the upper lid 2 and the culture vessel body 5 so that the upper lid 2 is in close contact with the culture vessel body 5. However, in the culture apparatus 1 of the present invention, since the culture vessel body 5 itself functions as a gasket or packing, the gasket or packing is intentionally installed. It is not necessary to adopt.
次に、槽内部品４としては、前記したもの以外に、例えば、各種センサー、熱交換コイル、サンプリング管、バスケット等を挙げることができる。
又、培養槽本体５は、シリコーンゴムから形成されているので、ガスケット乃至パッキンを介することなく上蓋２と、上蓋固定装置３により分解可能に連結されている。
以下、培養装置１の構成をさらに詳述する。
Next, examples of the in-vessel component 4 include various sensors, heat exchange coils, sampling tubes, baskets and the like in addition to those described above.
Moreover, since the culture tank main body 5 is formed of silicone rubber, it is connected to the upper lid 2 and the upper lid fixing device 3 so as to be disassembled without using a gasket or packing.
Hereinafter, the configuration of the culture apparatus 1 will be described in more detail.
上蓋２は、例えば、ステンレスの板材を用いて形成され、図１に示すように、攪拌機２１等が取り付けられている。
図２に示すように、上蓋２は、培養槽本体５の上部に載置され、上蓋固定装置３により、分解可能に連結することができる。上蓋２と培養槽本体５とは、ガスケット乃至パッキンを介することなく、上蓋固定ナット３１及び上蓋固定用ネジ金具３２とからなる上蓋固定装置３によって直接結合されている。
The upper lid 2 is formed using, for example, a stainless steel plate, and a stirrer 21 and the like are attached as shown in FIG.
As shown in FIG. 2, the upper lid 2 is placed on the upper part of the culture tank main body 5 and can be connected to the upper lid fixing device 3 so as to be disassembled. The upper lid 2 and the culture vessel main body 5 are directly coupled by the upper lid fixing device 3 including the upper lid fixing nut 31 and the upper lid fixing screw metal fitting 32 without using a gasket or packing.
培養槽本体５は、底付き円筒状の容器である。本実施例においては上部の開口周縁に水平方向により厚みを持たせた上縁部５１が設けられている。この上縁部５１に上蓋固定装置３を上下に通すことによって上蓋２と培養槽本体５とは分解可能に連結されている。 The culture tank main body 5 is a cylindrical container with a bottom. In the present embodiment, an upper edge 51 having a thickness in the horizontal direction is provided on the periphery of the upper opening. By passing the upper lid fixing device 3 up and down through the upper edge portion 51, the upper lid 2 and the culture tank main body 5 are detachably connected.
培養槽本体５の材料であるシリコーンゴムは、ガスケット乃至パッキンの物性を有するものが用いられている。又、耐熱、耐薬品性の材質のもので、繰り返しの蒸気滅菌でも劣化しにくい部材からなっており、常温で酸、アルカリ、塩素などへの耐性も強い部材である。又、比重も、ステンレスやガラスと比べて非常に小さい。 As the silicone rubber that is a material of the culture tank body 5, a material having physical properties of a gasket or packing is used. In addition, it is made of a heat-resistant and chemical-resistant material, and is made of a material that is not easily deteriorated even by repeated steam sterilization. The material is also highly resistant to acids, alkalis, chlorine, etc. at room temperature. The specific gravity is also very small compared to stainless steel and glass.
本発明の最も好適な実施例においては、培養槽本体５は全体が高透明のシリコーンゴムであるが、必ずしも全体が高透明である必要はなく、少なくとも一部に内部観察用の窓として内部監視用の透明の部分が設けられても良い。ここで高透明とはガラスやアクリル樹脂のような透明感をもった高透明なシリコーンゴムを言う。
本発明の培養槽本体で用いられる透明のシリコーンゴムとしては多少の白濁感があっても構わない。しかし、本発明においては、ガラスのような透明感を持った、いわゆる高透明シリコーンゴムが最も好ましい。この理由は、培養槽の内部観察が極めて容易であることや、意匠的な美観からである。
In the most preferred embodiment of the present invention, the culture vessel main body 5 is made of highly transparent silicone rubber as a whole, but it is not necessarily required to be highly transparent as a whole. A transparent part may be provided. Here, highly transparent means highly transparent silicone rubber having a transparent feeling like glass or acrylic resin.
The transparent silicone rubber used in the culture vessel body of the present invention may have a slight cloudiness. However, in the present invention, so-called highly transparent silicone rubber having a transparent feeling like glass is most preferable. This is because the inside observation of the culture tank is very easy and from the design aesthetics.
図２に示すように、培養槽本体５の上縁部５１には貫通孔５２が培養槽本体５の水平方向に形成されている。この貫通孔５２に槽内部品４の一部である配管が嵌め込まれている。培養槽本体５の貫通孔５２の部分はガスケット乃至パッキンとしても使用される物性を持つシリコーンゴムであるため、槽内部品４は培養槽本体５にしっかりと保持されると共に、培養槽本体５の内部は気密、液密に保たれる。
本発明の培養装置１においては、あえて上蓋２と培養槽本体５との間を気密、液密に密閉するために用いられるガスケット乃至パッキンは不要であり用いられていない。
又、培養槽本体５においても槽内部品４の配管用のガスケット乃至パッキンは不要であり用いられていない。
貫通孔５２は、目的とする培養の種類と槽内部品の種類に応じて、その口径や形状は適宜設計変更でき、又、複数個形成されても良い。本実施例では断面円形で、口径は槽内部品の一部である配管より、僅かに小さく形成されている。
As shown in FIG. 2, a through hole 52 is formed in the upper edge portion 51 of the culture tank body 5 in the horizontal direction of the culture tank body 5. A pipe which is a part of the in-vessel part 4 is fitted into the through hole 52. Since the portion of the through-hole 52 of the culture tank body 5 is made of silicone rubber having physical properties that are also used as a gasket or packing, the in-vessel component 4 is firmly held by the culture tank body 5 and the culture tank body 5 The interior is kept airtight and liquid tight.
In the culture apparatus 1 of the present invention, a gasket or packing used for sealing the space between the upper lid 2 and the culture tank body 5 in an airtight and liquid tight manner is unnecessary and not used.
Further, in the culture tank main body 5, a gasket or packing for piping of the tank internal part 4 is unnecessary and not used.
The diameter and shape of the through-hole 52 can be changed as appropriate according to the type of the desired culture and the type of the components in the tank, and a plurality of through-holes 52 may be formed. In this embodiment, the cross-section is circular, and the diameter is slightly smaller than the pipe that is a part of the in-tank part.
貫通孔５２の数が複数個設けられた場合には、槽内部品４の一部によって塞がれた貫通孔５２以外の貫通孔については、図示しない栓によって塞ぐことが可能である。 When a plurality of the through holes 52 are provided, the through holes other than the through holes 52 blocked by a part of the in-vessel part 4 can be closed by plugs (not shown).
なお、本発明の実施例では上縁部５１の横方向の厚みが他の部分と比べて厚みが大きくなっているが、同じ厚みであっても良い。 In addition, in the Example of this invention, although the thickness of the horizontal direction of the upper edge part 51 is large compared with another part, the same thickness may be sufficient.
上蓋２と培養槽本体５とは、上蓋固定ナット３１及び上蓋固定用ネジ金具３２とからなる上蓋固定装置３により分離可能に締結されている。 The upper lid 2 and the culture vessel main body 5 are fastened to each other by an upper lid fixing device 3 including an upper lid fixing nut 31 and an upper lid fixing screw 32.
本発明の培養装置は、動物細胞、植物細胞、微生物等の培養装置として利用可能である。 The culture apparatus of the present invention can be used as a culture apparatus for animal cells, plant cells, microorganisms and the like.
１ 培養装置
２ 上蓋
２１ 撹拌機
３ 上蓋固定装置
４ 槽内部品
５ 培養槽本体
５１ 上縁部
５２ 貫通孔
DESCRIPTION OF SYMBOLS 1 Culture apparatus 2 Upper lid 21 Stirrer 3 Upper lid fixing apparatus 4 In-tank part 5 Culture tank main body 51 Upper edge part 52 Through-hole
Claims (2)
前記培養槽本体がシリコーンゴムからなり、且つ、
前記培養槽本体には前記槽内部品を設置するための少なくとも１以上の貫通孔が形成されており、且つ
前記槽内部品の一部が前記貫通孔に嵌め込まれて保持されることを特徴とする培養装置。 In the culture apparatus including the upper lid, the tank inner part, the upper lid fixing device, and the culture tank body,
The culture vessel body is made of silicone rubber, and
The culture tank main body is formed with at least one or more through-holes for installing the in-tank part, and a part of the in-tank part is fitted into the through-hole and held. Culture device to do.
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| JP2017077195A (en) * | 2015-10-20 | 2017-04-27 | 株式会社日立製作所 | Culture tank structure |
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| WO2017069211A1 (en) * | 2015-10-20 | 2017-04-27 | 株式会社日立製作所 | Culture tank structure and culture vessel |
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