JP2014532406A - 組換えタンパク質発現のための操作された下等真核宿主株 - Google Patents

組換えタンパク質発現のための操作された下等真核宿主株 Download PDF

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Publication number
JP2014532406A
JP2014532406A JP2014538873A JP2014538873A JP2014532406A JP 2014532406 A JP2014532406 A JP 2014532406A JP 2014538873 A JP2014538873 A JP 2014538873A JP 2014538873 A JP2014538873 A JP 2014538873A JP 2014532406 A JP2014532406 A JP 2014532406A
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Japan
Prior art keywords
host cell
att1
polypeptide
seq
activity
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Pending
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JP2014538873A
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English (en)
Japanese (ja)
Inventor
ジアーン，ボー
アーギロス，レベツカ，デイー
ネルソン，ステフアニー
デイビツドソン，ロバート，シー
チエン，ローンフア
ジユアーン，ジユイン
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Merck Sharp and Dohme LLC
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Merck Sharp and Dohme LLC
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Application filed by Merck Sharp and Dohme LLC filed Critical Merck Sharp and Dohme LLC
Publication of JP2014532406A publication Critical patent/JP2014532406A/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2014538873A 2011-10-28 2012-10-23 組換えタンパク質発現のための操作された下等真核宿主株 Pending JP2014532406A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161552674P 2011-10-28 2011-10-28
US61/552,674 2011-10-28
PCT/US2012/061432 WO2013062940A1 (en) 2011-10-28 2012-10-23 Engineered lower eukaryotic host strains for recombinant protein expression

Publications (1)

Publication Number Publication Date
JP2014532406A true JP2014532406A (ja) 2014-12-08

Family

ID=48168381

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014538873A Pending JP2014532406A (ja) 2011-10-28 2012-10-23 組換えタンパク質発現のための操作された下等真核宿主株

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Country Link
US (1) US9328367B2 (https=)
EP (1) EP2771461B1 (https=)
JP (1) JP2014532406A (https=)
KR (1) KR20140091018A (https=)
CN (1) CN104024409A (https=)
AU (1) AU2012329091A1 (https=)
CA (1) CA2853341A1 (https=)
IN (1) IN2014CN02963A (https=)
WO (1) WO2013062940A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018038286A (ja) * 2016-09-05 2018-03-15 国立大学法人神戸大学 新規宿主細胞及びそれを用いた目的タンパク質の製造方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101790802B1 (ko) 2009-09-03 2017-10-27 머크 샤프 앤드 돔 코포레이션 항-ｇｉｔｒ 항체
CA2853341A1 (en) 2011-10-28 2013-05-02 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression
CN104105789A (zh) * 2012-02-16 2014-10-15 默沙东公司 降低酵母中甘露糖基转移酶活性的方法
CA2884573A1 (en) * 2012-10-22 2014-05-01 Merck Sharp & Dohme Corp. Crz1 mutant fungal cells
SG11201707056UA (en) * 2015-03-03 2017-09-28 Sandoz Ag Constitutive yeast LLP promoter-based expression system
WO2017194699A1 (en) * 2016-05-13 2017-11-16 University Of Copenhagen A cell-based array platform

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1194756A4 (en) * 1999-04-21 2004-12-29 Wyeth Corp METHODS OF IDENTIFYING N-TYPE ION CHANNEL INACTIVATION MODULATORS
US20060148000A1 (en) * 1999-04-21 2006-07-06 Wyeth Methods for identifying modulators of N-type ion channel inactivation
JP2004527204A (ja) * 2000-04-24 2004-09-09 ワイエス ペプチド結合対の特異的相互作用を有する新規な細胞系
EP1283265B1 (en) 2000-05-17 2009-08-05 Mitsubishi Tanabe Pharma Corporation Process for producing protein with reduction of mannose phosphate in the sugar chain and glycoprotein produced thereby
ES2252261T3 (es) 2000-06-28 2006-05-16 Glycofi, Inc. Metodos para producir glicoproteinas modificadas.
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
CA2551484C (en) 2003-12-24 2015-03-31 Glycofi, Inc. Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
CN101084233B (zh) * 2004-04-29 2012-08-15 格利科菲公司 用于在糖蛋白产生中减少或消除α-甘露糖苷酶抗性聚糖的方法
CN101606769A (zh) 2009-07-15 2009-12-23 陈德芳 一种宝石连接结构
AR087433A1 (es) * 2011-08-08 2014-03-26 Merck Sharp & Dohme Analogos de insulina n-glicosilados
CA2853341A1 (en) 2011-10-28 2013-05-02 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [ONLINE], ACCESSIN NO. FR839628, <HTTP://WWW.NCBI.NLM.NIH.GOV/NUCCORE/328349772?SAT, JPN6016031931 *
NUCLEIC ACIDS RES. (1993) VOL.21, NO.1, P.69-77, JPN6016031928 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018038286A (ja) * 2016-09-05 2018-03-15 国立大学法人神戸大学 新規宿主細胞及びそれを用いた目的タンパク質の製造方法

Also Published As

Publication number Publication date
KR20140091018A (ko) 2014-07-18
US9328367B2 (en) 2016-05-03
EP2771461A4 (en) 2015-06-17
WO2013062940A1 (en) 2013-05-02
CN104024409A (zh) 2014-09-03
EP2771461B1 (en) 2019-12-04
US20140302557A1 (en) 2014-10-09
CA2853341A1 (en) 2013-05-02
IN2014CN02963A (https=) 2015-07-03
EP2771461A1 (en) 2014-09-03
AU2012329091A1 (en) 2014-05-01

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