CN104024409A - 用于重组蛋白表达的经工程改造的低等真核宿主菌株 - Google Patents

用于重组蛋白表达的经工程改造的低等真核宿主菌株 Download PDF

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Publication number
CN104024409A
CN104024409A CN201280064747.2A CN201280064747A CN104024409A CN 104024409 A CN104024409 A CN 104024409A CN 201280064747 A CN201280064747 A CN 201280064747A CN 104024409 A CN104024409 A CN 104024409A
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China
Prior art keywords
host cell
att1
seq
polypeptide
activity
Prior art date
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Pending
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CN201280064747.2A
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English (en)
Chinese (zh)
Inventor
B.江
R.D.阿吉罗斯
S.尼尔森
R.C.戴维德森
R.陈
J.庄
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Merck Sharp and Dohme LLC
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Schering Corp
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Publication date
Application filed by Schering Corp filed Critical Schering Corp
Publication of CN104024409A publication Critical patent/CN104024409A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201280064747.2A 2011-10-28 2012-10-23 用于重组蛋白表达的经工程改造的低等真核宿主菌株 Pending CN104024409A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161552674P 2011-10-28 2011-10-28
US61/552674 2011-10-28
PCT/US2012/061432 WO2013062940A1 (en) 2011-10-28 2012-10-23 Engineered lower eukaryotic host strains for recombinant protein expression

Publications (1)

Publication Number Publication Date
CN104024409A true CN104024409A (zh) 2014-09-03

Family

ID=48168381

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280064747.2A Pending CN104024409A (zh) 2011-10-28 2012-10-23 用于重组蛋白表达的经工程改造的低等真核宿主菌株

Country Status (9)

Country Link
US (1) US9328367B2 (https=)
EP (1) EP2771461B1 (https=)
JP (1) JP2014532406A (https=)
KR (1) KR20140091018A (https=)
CN (1) CN104024409A (https=)
AU (1) AU2012329091A1 (https=)
CA (1) CA2853341A1 (https=)
IN (1) IN2014CN02963A (https=)
WO (1) WO2013062940A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101790802B1 (ko) 2009-09-03 2017-10-27 머크 샤프 앤드 돔 코포레이션 항-ｇｉｔｒ 항체
CA2853341A1 (en) 2011-10-28 2013-05-02 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression
CN104105789A (zh) * 2012-02-16 2014-10-15 默沙东公司 降低酵母中甘露糖基转移酶活性的方法
CA2884573A1 (en) * 2012-10-22 2014-05-01 Merck Sharp & Dohme Corp. Crz1 mutant fungal cells
SG11201707056UA (en) * 2015-03-03 2017-09-28 Sandoz Ag Constitutive yeast LLP promoter-based expression system
WO2017194699A1 (en) * 2016-05-13 2017-11-16 University Of Copenhagen A cell-based array platform
JP2018038286A (ja) * 2016-09-05 2018-03-15 国立大学法人神戸大学 新規宿主細胞及びそれを用いた目的タンパク質の製造方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081548A1 (en) * 2000-04-24 2001-11-01 Wyeth Novel cell systems having specific interaction of peptide binding pairs
US20020139012A1 (en) * 1999-04-21 2002-10-03 American Home Products Corporation Methods for identifying modulators of N-type ion channel inactivation
US20060148000A1 (en) * 1999-04-21 2006-07-06 Wyeth Methods for identifying modulators of N-type ion channel inactivation
US20060211085A1 (en) * 2004-04-29 2006-09-21 Piotr Bobrowicz Methods for reducing or eliminating alpha-mannosidase resistant glycans for the production of glycoproteins
CN103889444A (zh) * 2011-08-08 2014-06-25 默沙东公司 N-糖基化的胰岛素类似物

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1283265B1 (en) 2000-05-17 2009-08-05 Mitsubishi Tanabe Pharma Corporation Process for producing protein with reduction of mannose phosphate in the sugar chain and glycoprotein produced thereby
ES2252261T3 (es) 2000-06-28 2006-05-16 Glycofi, Inc. Metodos para producir glicoproteinas modificadas.
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
CA2551484C (en) 2003-12-24 2015-03-31 Glycofi, Inc. Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
CN101606769A (zh) 2009-07-15 2009-12-23 陈德芳 一种宝石连接结构
CA2853341A1 (en) 2011-10-28 2013-05-02 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020139012A1 (en) * 1999-04-21 2002-10-03 American Home Products Corporation Methods for identifying modulators of N-type ion channel inactivation
US20060148000A1 (en) * 1999-04-21 2006-07-06 Wyeth Methods for identifying modulators of N-type ion channel inactivation
WO2001081548A1 (en) * 2000-04-24 2001-11-01 Wyeth Novel cell systems having specific interaction of peptide binding pairs
US20060211085A1 (en) * 2004-04-29 2006-09-21 Piotr Bobrowicz Methods for reducing or eliminating alpha-mannosidase resistant glycans for the production of glycoproteins
CN103889444A (zh) * 2011-08-08 2014-06-25 默沙东公司 N-糖基化的胰岛素类似物

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KUBERL A. ET AL.: "Pichia pastoris CBS 7435 chromosome 1, complete replicon sequence", 《GENBANK》 *
KUBERL A. ET AL.: "Pichia pastoris CBS 7435 chromosome 1, complete replicon sequence", 《GENBANK》, 6 April 2011 (2011-04-06) *
W.ZACHARIAE ET AL.: "Glucose repression of lactose/galactose metabolism in Kluyv/eromyces lactis is determined by the concentration of the transcriptional activator LA1 9 (KiGAL4)", 《NUCLEIC ACIDS RESEARCH》 *

Also Published As

Publication number Publication date
KR20140091018A (ko) 2014-07-18
US9328367B2 (en) 2016-05-03
JP2014532406A (ja) 2014-12-08
EP2771461A4 (en) 2015-06-17
WO2013062940A1 (en) 2013-05-02
EP2771461B1 (en) 2019-12-04
US20140302557A1 (en) 2014-10-09
CA2853341A1 (en) 2013-05-02
IN2014CN02963A (https=) 2015-07-03
EP2771461A1 (en) 2014-09-03
AU2012329091A1 (en) 2014-05-01

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