JP2014525906A - Novel nanogold and its preparation process - Google Patents

Abstract

The present application relates to the biological synthesis of novel nanogold by an environmentally friendly process using plant raw materials classified into the genus Crassica, Mikan, Ouyuki, Dolichos, and Zeocalpama. The novel nanogold product of the present invention is not limited to human and animal treatments, pharmaceutical compositions, cosmetics, herbal compositions, but technical fields such as nanotechnology, environmental protection technology, and biotechnology. Used for The invention also relates to the use of plant raw materials in metal purification and particle size reduction.
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Description

  The present invention relates to an environmentally friendly process for obtaining nanogold. The present invention broadens the application range of gold in therapy. The product, which is a novel nanogold obtained by novel biological synthesis using plant materials, has increased adsorption capacity and low toxicity upon oral administration. The novel product can be administered with or without an appropriate carrier and can be administered with other plant materials or therapeutic compounds. The invention also applies to nanotechnology, biotechnology, environmental protection technology, and material science.

  The object of the present invention is the biological synthesis of nanogold for oral administration with thermal stability, low toxicity, particle size in the range of 10 nm-1000 nm and enhanced adsorption capacity in human and animal treatment Related to manufacturing. The present invention is a fusion of biotechnology and environmental protection technology to produce nanogold without producing harmful waste. The novel nanogold is also applied in various fields including, but not limited to, technical fields that take into account its stability, strong properties, particle size, and low toxicity. It is a further object of the present invention to establish an environmentally friendly nanogold production process that does not produce harmful waste.

  The applicability of gold in therapy has been known for a long time. Orally administrable gold based products are known in traditional and alternative medicine. Furthermore, the gold-based products obtained by the chemical reduction process are ingestible and injectable into the body and can also be used for the treatment of humans and animals. Gold salts obtained by chemical reduction are known to cause side effects due to their toxicity, so their therapeutic application is limited. Despite chemical purification, impurities are found on the surface of the gold particles, and when these impurities are administered to humans and animals, they cause various side effects. The toxicity of gold-based products causes side effects such as nausea, vomiting, diarrhea, dermatitis, and blood disorders. The main reason for toxicity is the change in oxidation state and carrier molecule after administration. For example, orally administrable gold products obtained by chemical reduction, such as auranofin or lidaura, are known to have a less therapeutic effect than injectable gold salts.

  The advent of nanogold particles obtained by chemical reduction such as underwater arc discharge, electrochemical processes, etc. has improved the applicability of gold in therapy. However, the colloidal gold obtained by the chemical reduction process results in stable gold colloids and partially stable gold colloids or gold nanosuspensions that are less effective when administered orally. It has been found. Although the nanogold product obtained by electrochemical process is patent pending, it has been described that having a cleaner surface increases its effectiveness in vivo and the particle size range is 8 nm to 100 nm. (International application PCT / US2010 / 041427).

  Gold-based products are known for their anti-inflammatory properties and bacteriostatic effects and are used in the treatment of rheumatoid arthritis, tuberculosis, cancer, asthma, HIV, malaria and the like. However, toxicity and in vivo oxidative factors limit its use in animal and human therapy.

  In addition, stronger and more stable gold nanoparticles are one of the most preferred nanomaterials in the current state in technical applications, especially biomedical applications such as bioimaging.

  Advanced technology shows that there are various methods for producing nanogold. Except for the manufacturing process of “swarna bhasma”, there is no known other process for obtaining nanogold by biological synthesis. Swarna bhasma is an indispensable part of traditional medicine in India, including gold containing spherical particles with an average size of 56 nm-57 nm. Traditional methods are: (1) Sesamum Indicum Linn, buttermilk, cattle urine, soured straw processed from rice, and refinement using Dolichos biflorous Linn, (2) using hematite and rock salt Special refining and heating processes that had been (3) an ashing process by adding mercury and sulfur in the required ratio. The resulting product is a brownish red powder “swarna bhasma” (BrownCL et al, Gold Bulletin 2007).

  The production and use of “swarna bhasma” is further discussed in international application PCT / IN2008 / 000816, where “swarna bhasma” produced by traditional methods is used as a way to perpetuate stem cell nature in stem cell therapy. Be charged. However, the processes and products discussed in the above application do not resemble the present invention.

  In order to expand the applicability of metallic gold, there is a need for nanogold that is stable and more powerful, has high mobility, adequate therapeutic effect particle size, and low toxicity.

  The novel nanogold obtained by the biological synthesis process of the present invention is therapeutically effective but non-toxic when administered at a specific dose. Due to its small particle size and faster mobility, it is known to cross the blood brain barrier and blood placenta barrier. It is also known to induce spontaneous cell death of cancer cells. In particular, the novel process is carried out at various stages using plant materials. There is no process similar to the process claimed here.

  Stage I of the process is to purify metallic gold using Dalbergia Sissoo and Dolichos Biflorous. Stage II relates to the refined gold particle size reduction utilizing lime (Citrus Acida) and green fennel (Ferula). Stage III is ashing to produce purified nanogold with a size range of 10 nm-1000 nm.

  The present invention is a novel biological synthesis that differs from any existing technology, including processes associated with the production of “swarna bhasma”. The special refining processes utilizing mercury and sulfur, which are different plant materials used and emphasized in the production of swarna bhasma, are not part of the present invention. Furthermore, in the present invention, the ashing temperature and process are different. In the present invention, Himalayan wisteria bean is used independently of any other plant substance or organic substance in the purification stage after boiling metal gold with Sisson. Traditional methods include using Himalayan wisteria with cow urine, buttermilk and the like.

  The new product obtained by the new process is pinkish brown or yellow, depending on the desired particle size. The new product is thermally stable and is not toxic when administered at specific doses.

  This new product has better therapeutic applicability than any existing gold salt and nanogold particles, especially in the treatment of cancer. The novel products have application in a variety of other human and animal treatments including, but not limited to, treatment of rheumatoid arthritis, asthma and the like. The novel nanogold is also applied as an anti-inflammatory, aphrodisiac, and antioxidant. The application of novel nanogold in herbal products, medicinal cosmetics, and nutritional supplements is also anticipated.

  Administration can be with a suitable carrier, such as honey, water, botanical ingredients, minerals, or combinations thereof. The therapeutic effect and toxicity can vary depending on the type of carrier used for administration. In the present invention, it is also envisaged to administer the novel nanogold without using a suitable carrier.

  In the present invention, novel nanogold applications in fields such as material science, biochemical applications, biotechnology, nanotechnology, environmental conservation technology, and agriculture are expected.

  The present invention relates to the biological synthesis of nanogold particles using plant materials at different stages of purification, grinding and ashing. The present invention relates to a process for obtaining nanogold by biological synthesis performed in three stages. That is, in order to reduce the particle size together with other plant raw materials such as Citrus and Ouenkyo, the first stage of biological refining of metallic gold foil using the extracted components of Sissonoki and Himalayan wisteria The second stage of milling is the third stage of heating to produce new nanogold that is not toxic when administered at a specific dose, can be administered orally, and has a higher adsorption capacity. The essence of the present invention includes the use of Sissonoki in the metal refining process and the use of Citrus and Oenothera plant materials in the process of reducing particle size. This was not known in the prior art.

  The novelty of the present invention also relates to particle size. The process of the present invention produces new nanogold with a size of 10 nm-1000 nm by biological synthesis. The present invention is not only a novel biological process for producing nanogold, but also includes a novel use of plant materials in metal purification and metal particle size reduction. In the metal gold refining process performed before particle size reduction, plant materials classified as genus “Dalbergia”, plant materials classified as specific Sissonoki, or others having the characteristics of the above compounds The use of any neoflavonoid has not been previously known. The present invention uses a mixture of equal amounts of liquid lime and dried Oowei by weight to reduce the particle size of metal gold purified by biological synthesis, and has a size of 10 nm to 1000 nm. Provide nano-gold. It is also anticipated that gold purified by any other method that yields the same quality as the Stage I product will be used for particle size reduction using lime and mallow. The use of any plant material classified as "Tangerus" and "Ouyoukyo" is also envisaged in the present invention. Furthermore, it is envisaged that plant raw materials or chemical compounds having similar properties may be used in the metal purification process and particle size reduction process described above.

  Himalayan wisteria is often used as an astringent, diuretic, and tonic. Enzymes found in Himalayan peas identified as urease, allantoinase, ribonuclease, nicotinamide deaminase, β-N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, and β-glucosidase Has been. In the present invention, metal gold is boiled with Sisson and then used during the purification stage, which is a novel application.

  Limes classified in the genus “Tangerine” are commonly found in Asia. Lime juice contains citric acid, malic acid, phosphoric acid, and salt. The medicinal properties of lime are well known and are also used as ingredients. The use of lime in reducing metal particle size is not known in the state of the art. The present invention utilizes lime juice to reduce metal gold particle size through biological synthesis. Lemon, Citron, Daidai, Garcinia Pedunculata, etc. plants under the genus mandarin, or any other plant material including other ingredients found in citric acid and lime, or compounds that have similar effects Chemical compounds are envisioned in the present invention.

  Plant raw materials classified as Ouyoukyo are known for their medicinal properties and are also used as food ingredients. Ferula Narthex, Ferula Asafoetida, and Ferula Foetida are generally plants native to Iran and Afghanistan and have been widely used in traditional medicine since ancient times. However, it is not known in the state-of-the-art medicine or traditional medicine to combine orange fennel and lime juice for metal particle size reduction. The present invention establishes a unique and non-obvious use of plant materials classified into the genus "Ouyoukyo" and "Tangerine".

  The following description of the process should not be construed as limiting. In one embodiment of the invention, Ferula Narthex and lime are used together in an appropriate ratio to reduce the particle size of the refined metallic gold.

  Stage I of the process relates to the biological purification of metallic gold and is the first stage of the nanogold manufacturing process. The foil or other shaped metal gold is heated, preferably at about 600 ° C., until it becomes red hot. A temperature of 600 ° C. should not be construed as limiting. The red hot metal gold is boiled in the extracted oil of Sissonoki for several hours, preferably at least 7 hours. The time of at least 7 hours required for boiling should not be construed as limiting. The resulting product obtained after boiling with Sissonoki is then boiled for several hours (but not limited to at least 7 hours) in the extracted components of Himalayan Fuji beans to obtain a metal powder. In order to obtain gold that has been organically purified by biological synthesis, the product, that is, the metal powder, is subjected to a boiling process using Sissonoki and a boiling process in the extracted components of Himalayan Fuji beans several times. This process needs to be repeated 10 times, but the number of process iterations of 10 should not be interpreted as a natural process.

  Stage II of the new process relates to grinding. The product of stage I is ground to dryness at a temperature between 22 ° C. and 45 ° C. with a mixture of equal weight ratios of lime juice and dried Ferula Narthex. The resulting dried product is subjected to at least 10 additional pulverization processes with a mixture of lime and Ferula Narthex to achieve the optimal and finely divided desired particle size. The ratio of lime juice to dried Ferula Narthex in the mixture should be equal by weight. The number of process iterations of 10 should not be construed as limiting. The desired particle size is a determinant of the number of repetitions.

  Stage III of the process of the present invention relates to ashing. After reaching the optimum particle size, the dried product is exposed to high temperatures in the range of 300 ° C. to 950 ° C. until it is dried and gradually cooled. The minimum temperature when exposed to high temperature is preferably 300 ° C. This process of heating and cooling is repeated several times, at least 10 times, until a dry nanogold product is obtained. The color of the product varies from mild pinkish brown to yellow depending on the desired particle size. The number of times the process is repeated until a product of the desired particle size is obtained should not be construed as limiting the process. In order to ensure the optimal range of heating and cooling, biomass, usually cow dung, is used as the heating fuel. The use of biomass ensures that the optimum maximum temperature is obtained until a dry product is obtained before gradual cooling. This ashing process is performed in an apparatus that provides uniform heating.

  The new nanogold dusts, pinkish brown or yellow, are biologically pure and have a particle size range of 10 nm-1000 nm.

  The dosage of new nanogold is usually in the range of 1 microgram to 100 mg per day. The dose specified as 1 microgram to 100 mg should not be construed as limiting because the dose can vary depending on various factors. Specific dosages of other minerals that can be administered in combination with the novel nanogold are usually in the range of 1 microgram to 120 mg. The dosage of nanogold and other minerals and plant materials varies depending on various factors such as the general health condition and age of the patient, and in the case of a tumor, the type and progression stage of the tumor. Accordingly, the specific dosage should not be construed as limiting in any case.

  Honey and water are most widely used as suitable carriers, but should not be construed as limiting as any other suitable carrier can be used for administration.

  New nanogold medicinal, nutraceutical, cosmetic, or herbal compositions or dosage forms suitable for administration may be powders, tablets, effervescent, liquid, gelatin, granules, or any other good taste It may be in an administrable form.

New nanogold medicinal, nutraceutical, cosmetic, or herbal compositions or dosage forms suitable for administration may be powders, tablets, effervescent, liquid, gelatin, granules, or any other good taste It may be in an administrable form.
(Item 1)
(A) heating the metal gold until red hot;
(B) boiling the red-hot metal gold together with the extractive component of Sissonoki,
(C) boiling the metal gold that has undergone step (b) with the extract of Himalayan wisteria;
(D) grinding the product obtained by step (c) with a mixture of lime and Ferula Narthex until dry;
(E) ashing the product obtained by step (d) by exposing the product obtained by step (d) to a gradually increasing temperature until an optimum maximum temperature is obtained; ,
(F) gradually cooling the product of step (e) to dryness to obtain nanogold.
(Item 2)
Plant raw materials classified into the genus Crane genus in step (b), macrorotomas in step (c), plant raw materials classified into the genus Citrus and Oenothera in step (d), or extract components thereof, Alternatively, a product obtained by the process according to item 1, obtained organically or otherwise and using compounds having similar properties.
(Item 3)
Use of a plant raw material that is expected to produce a desired effect in order to obtain nanogold in the process from stage (a) to stage (g) according to item 1.
(Item 4)
In the step (b) according to item 1 and the metal purification of a plant raw material belonging to the genus Crane or sisson, its extract, Dalbergia Chromene, or a neoflavonoid obtained by organic, chemical or other processes Independent use, or use with other plant materials or chemical compounds.
(Item 5)
Himalayan wisteria and other botanical raw materials, or their extracted components, or chemical or combined with plant raw materials classified as genus Crane or their extract components, or neoflavonoids obtained by chemical or other processes Use in step (c) of the process according to item 1 of a compound obtained by other processes and having the same or similar properties as Himalayan wisteria.
(Item 6)
Any plant material classified as lime or Citrus genus, or an extract thereof, or a compound obtained by chemical or other processes and having the same or similar characteristics as plant materials classified as Citrus Independent use in metal particle size reduction and step (d) as described in item 1, or in combination with Ferula Narthex, or plant raw materials classified as Ouary, extracts thereof, or chemicals Or use with compounds obtained by other processes and having the same or similar properties as plant raw materials classified in the genus Pleurotus.
(Item 7)
Use of the product according to item 2 in nanotechnology, material science, biotechnology, environmental protection technology and other applicable fields.
(Item 8)
Use of a product according to item 2 in a pharmaceutical composition, a nutritional supplement composition, a cosmetic, a herbal composition or a treatment.
(Item 9)
Direct or indirect administration and use of the product according to item 2 in the treatment of humans and animals.
(Item 10)
Item 1. The process of item 1 and the application of the product of item 2 defined by its environmental friendliness, low toxicity, thermal stability, strong properties and particle size.
(Item 11)
Item 3. Product dosage form according to item 2, in any form that is well administrable for animal and human treatment.

Claims (11)

(A) heating the metal gold until red hot;
(B) boiling the red-hot metal gold together with the extractive component of Sissonoki;
(C) boiling the metal gold after the step (b) together with an extract of Himalayan wisteria;
(D) grinding the product obtained by said step (c) with a mixture of lime and Ferula Narthex until dry;
(E) ashing the product obtained by step (d) by exposing the product obtained by step (d) to a gradually increasing temperature until an optimum maximum temperature is obtained. And the stage of
(F) a process of obtaining nanogold comprising: gradually cooling the product of step (e) to dryness.   Plant raw materials classified into the genus Crane genus in the step (b), Zeocarpa bean (Macrotyloma) in the step (c), Plant raw materials classified into the genus Citrus and Ouenki in the step (d), or these 2. A product obtained by the process of claim 1 using an extractive component or a compound obtained organically or otherwise and having similar properties.   Use of a plant material expected to produce a desired effect for obtaining nanogold in the process from step (a) to step (g) of the process according to claim 1.   The step (b) according to claim 1 and the metal of the plant raw material belonging to the genus Crane or Sisson, its extract, Dalbergia Chromene, or a neoflavonoid obtained by organic, chemical or other processes. Independent use in refining or use with other plant materials or chemical compounds.   Himalayan wisteria and other botanical raw materials, or their extracted components, or chemical or combined with plant raw materials classified as genus Crane or their extract components, or neoflavonoids obtained by chemical or other processes Use of a compound obtained by other processes and having the same or similar properties as Himalayan wisteria in step (c) of the process according to claim 1.   Any plant material classified as lime or Citrus genus, or an extract thereof, or a compound obtained by chemical or other processes and having the same or similar characteristics as plant materials classified as Citrus 2. Independent use in said step (d) according to claim 1, reduced in particle size of metal, or in combination with Ferula Narthex, or a plant raw material classified as Ouary, extract thereof, or Use with compounds obtained by chemical or other processes and having the same or similar properties as plant raw materials classified in the genus Pleurotus.   Use of the product according to claim 2 in nanotechnology, material science, biotechnology, environmental protection technology and other applicable fields.   Use of the product according to claim 2 in a pharmaceutical composition, a nutritional supplement composition, a cosmetic, an herbal composition or a treatment.   Direct or indirect administration and use of the product according to claim 2 in the treatment of humans and animals.   The process of claim 1 and the application of the product of claim 2 defined by its environmental friendliness, low toxicity, thermal stability, strong properties and particle size.   3. The dosage form of the product of claim 2 in any form that is well administrable for animal and human treatment.