JP2014507668A - 選択的に官能基化されたナノ流体バイオセンサーにおける生体分子の迅速な定量およびその方法 - Google Patents
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Abstract
Description
本発明の目的は、複雑な操作を必要としない、安価で迅速なナノ流体バイオセンサーを提供することである。
本発明は、選択的に官能基化された表面を有するナノメーターサイズの限局域に生体分子を進入させることが、生体分子が固定化されたバイオマーカーと相互作用する確率を大きく増大させるという発見に基づく。これは、蛍光標識生体分子の存在の超低濃度における定量を可能とする。
本明細書中で使用されるとおり、用語「生体分子」は、例えば、抗体またはサイトカイン、などのタンパク質、ペプチド、核酸、脂質分子、ポリサッカライドおよびウイルスを含む(が、それらに限定されない)、総称であることを意図する。
Claims (11)
- 2つの基材(201、202)の間に画定され、バイオマーカーが固定化される1つまたは数個の選択的に官能基化された領域(211)を有するナノチャンネル(210)を含む、蛍光標識生体分子(320)を検出し、定量化するためのバイオセンサー(200)であって、前記ナノチャンネルはさらに、側面インプット開口部(220)および側面アウトプット開口部(230)によって画定されており、前記インプット開口部(220)は、生体分子(320)を含有する溶液を前記ナノチャンネル(210)に進入させるように適合されており、前記アウトプット開口部(230)は、毛細管現象によって前記ナノチャンネル(210)を通して前記溶液を推進するように適合されている、前記バイオセンサー(200)。
- 前記バイオマーカー(310)が、特定の生体分子(320)と生物学的または化学的に相互作用し、および/または前記溶液(300)中に含有される非特異的生体分子(330)と相互作用しないように適合されている、請求項1に記載のバイオセンサー(200)。
- 前記基材(201、202)が、シリコン、ガラス、プラスチックおよび酸化物化合物から成る群から選ばれる材料から作られている、請求項1または2に記載のバイオセンサー(200)。
- 前記アウトプット開口部(230)が、前記ナノチャンネル(210)を通して前記溶液を推進するように適合された駆動要素(241または242)を有しているかまたはそれと接触している、請求項1〜3のいずれか1項に記載のバイオセンサー(200)。
- 前記ナノチャンネル(210)および前記側面開口部(220、230)内部の非官能基化表面が、1nmから1000nmの間の厚さを有する、金属化合物、プラスチック化合物および酸化物化合物から成る群から選ばれる材料の薄層を含む、請求項1〜4のいずれか1項に記載のバイオセンサー(200)。
- 前記側面開口部(220、230)が、100nm2から20mm2までの面積を有し、かつ前記ナノチャンネル(210)が、2nmから1000nmの高さ、2nmから20mmの幅、および2nmから20mmの長さを有する、請求項1〜5のいずれか1項に記載のバイオセンサー(200)。
- 請求項1〜6のいずれか1項に記載の数個のバイオセンサー(200)を含むアレイであって、前記バイオセンサー(200)がカプセルシステム(101)内部または表面(102)上に固定されている、前記アレイ。
- 請求項1〜7のいずれか1項に定義された1つまたは数個のバイオセンサー(200)から成り、かつ蛍光励起および検出のための光学的手段(500)を含む、アセンブリー。
- 前記光学的手段(500)が、検出器アレイ(CMOSもしくはCCD)、アバランシェフォトダイオード(APD)、または光電子増倍管(PMT)などの単一光子検出器である検出器を含む蛍光測定ユニットである、請求項8に記載のアセンブリー。
- 溶液(300)中の蛍光標識生体分子(320)の存在を検出し、定量化する方法であって、以下の:
a)少なくとも1つの、請求項1〜6のいずれか1項に定義されたバイオセンサー(200);
b)バイオマーカー(310)に対して特異的であることができ、前記ナノチャンネル中に固定化され、かつ、前記カプセルシステム(101)または前記表面(102)中には固定化されない標識生体分子(320および/または330)を含有する水溶液(300)を、側面インプット開口部(220)から前記側面アウトプット開口部(230)まで前記ナノチャンネル(210)を横切って置くことによる、前記バイオセンサー(200)の充填メカニズム;
c)光学システム(500);
d)前記ナノチャンネル(210)内部のバイオマーカー(310)上に固定化された特定の生体分子(320)に結合したフロオロフォアの光退色による前記生体分子(320)の検出、ならびに前記ナノチャンネル(210)の長さにわたる濃度勾配の決定、
を含む、前記方法。 - 前記生体分子(320)が、タンパク質、DNA、RNA、抗体、アミノ酸、核酸、酵素、脂質分子、ペプチド、ポリサッカライドまたはウイルスである、請求項10に記載の方法。
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PCT/IB2012/050527 WO2012120387A1 (en) | 2011-03-09 | 2012-02-06 | Rapid quantification of biomolecules in a selectively functionalized nanofluidic biosensor and method thereof |
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US (1) | US9547004B2 (ja) |
EP (1) | EP2684027B1 (ja) |
JP (1) | JP6130306B2 (ja) |
CN (1) | CN103502795B (ja) |
AU (1) | AU2012226463B2 (ja) |
BR (1) | BR112013022952B1 (ja) |
CA (1) | CA2829178C (ja) |
ES (1) | ES2767123T3 (ja) |
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JP2017524952A (ja) * | 2014-07-03 | 2017-08-31 | アビオニック・ソシエテ・アノニム | 全血などの流体サンプルの迅速分子定量化のためのカプセル |
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WO2011108540A1 (ja) | 2010-03-03 | 2011-09-09 | 国立大学法人大阪大学 | ヌクレオチドを識別する方法および装置、ならびにポリヌクレオチドのヌクレオチド配列を決定する方法および装置 |
EP3021968B1 (en) * | 2013-07-17 | 2018-09-26 | Abionic SA | Method and device for bioassays |
KR20160079780A (ko) | 2013-09-18 | 2016-07-06 | 퀀텀 바이오시스템즈 가부시키가이샤 | 생체분자를 서열화하는 디바이스들, 시스템들 및 방법들 |
JP2015077652A (ja) | 2013-10-16 | 2015-04-23 | クオンタムバイオシステムズ株式会社 | ナノギャップ電極およびその製造方法 |
BR112016013252B1 (pt) * | 2013-12-13 | 2020-09-29 | Abionic Sa | Sistema de evacuação de gás para biossensor nanofluídico e método para evacuação de gás de um sistema nanofluídico |
CN106414767A (zh) * | 2014-01-24 | 2017-02-15 | 量子生物有限公司 | 生物分子的测序装置、系统及方法 |
CN104651500B (zh) * | 2015-01-30 | 2017-06-30 | 华东理工大学 | 气单胞菌溶素纳米孔通道的制备方法及其应用 |
KR20180101595A (ko) | 2016-02-01 | 2018-09-12 | 프리벤치오 인코포레이티드 | 심혈관 질환 및 사건을 위한 진단학적 및 예후적 방법 |
CN113324958A (zh) * | 2021-05-19 | 2021-08-31 | 江南大学 | 一种可视化检测重金属汞的快检毛细管及其制备方法和应用 |
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JP6130306B2 (ja) | 2017-05-17 |
BR112013022952A2 (pt) | 2016-12-06 |
ES2767123T3 (es) | 2020-06-16 |
US20140256573A1 (en) | 2014-09-11 |
CA2829178A1 (en) | 2012-09-13 |
CN103502795B (zh) | 2016-02-24 |
EP2684027A1 (en) | 2014-01-15 |
AU2012226463A1 (en) | 2013-10-10 |
CN103502795A (zh) | 2014-01-08 |
EP2684027B1 (en) | 2019-12-11 |
AU2012226463B2 (en) | 2015-03-12 |
WO2012120387A1 (en) | 2012-09-13 |
CA2829178C (en) | 2020-01-07 |
US9547004B2 (en) | 2017-01-17 |
PT2684027T (pt) | 2020-01-27 |
HK1187982A1 (zh) | 2014-04-17 |
BR112013022952B1 (pt) | 2020-09-08 |
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