JP2014131968A - Radiation damage inhibiting composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide medicaments and food products for alleviating radiation damage to a person exposed to radiation, and methods of producing and administering the same.SOLUTION: A radiation damage inhibitor includes chlorellavulgaris powder as an active ingredient. More preferably, the radiation damage inhibitor includes chlorellavulgaris E-25 strain powder as an active ingredient.

Description

  The present invention provides pharmaceuticals and foods that reduce radiation damage to people exposed to radiation.

The Fukushima Daiichi nuclear power plant was damaged by the Great East Japan Earthquake and melted down, releasing radioactive materials into the atmosphere. In Fukushima Prefecture, there is an increased risk of damage from radiation exposure.
Although radiation therapy for cancer patients is effective, there is a problem of side effects because not only cancer cells but also normal cells are destroyed.

Accordingly, there is a need for drugs and foods that reduce side effects and radiation damage to cancer patients and people who have been exposed to radiation in nuclear accidents.
On the other hand, although the use of Chlorella genus that hardly contains α-cellulose in the cell wall is described in Patent Document 1, development of a useful application of such Chlorella genus has been demanded.

    Patent Document 1 Japanese Patent Publication No. 3-47836

  Provided are a medicine, a food, a production method thereof, and an administration method for reducing radiation damage to a person exposed to radiation.

According to the present invention, the following inventions are provided.
(1) A radiation injury inhibitor comprising chlorella vulgaris powder as an active ingredient.
(2) Chlorella vulgaris is E-25 strain | stump | stock, The radiation injury inhibitor of Claim 1 characterized by the above-mentioned.

  The radiation injury inhibitor of the present invention has an effect of reducing the damage even when exposed to radiation.

  The form of the medicine and food composition containing Chlorella vulgaris E-25 of the present invention can be used as it is as a culture or as a dried product, but it is obtained by disrupting cells by French press or ultrasonic treatment. It may be used. Alternatively, an extract using an organic solvent such as acetone, methanol, ethanol, hexane, or an aqueous extract may be used as it is or after the above treatment. Moreover, as long as an active ingredient is not impaired, you may refine | purify further by the refinement | purification means well-known to those skilled in the art, and may add and manufacture processes, such as decoloring and deodorizing.

  As a form of a medicine or a food composition, the following forms can also be used. For example, when used for food, sugar, fructose and other sugars, stabilizers, acidulants (citric acid, malic acid), vitamins, minerals, etc. are blended, tea, coffee, fruit juice drinks, fermented milk, It can be used in the form of carbonated beverages, pudding, jelly, drop, caramel, chocolate, sprinkle, dressing, soup and the like. When chlorella is blended with food, the blending ratio with respect to the food is 10 to 90% by weight, more preferably 20 to 50% by weight, and further preferably 20 to 30% by weight in terms of dry chlorella.

  Moreover, when using as a pharmaceutical, the dosage form can be selected arbitrarily. In general, it is formulated with a pharmaceutically acceptable liquid or solid carrier and, if necessary, a solvent, a dispersant, an emulsifier, a buffer, a stabilizer, an excipient, an extender, a binder, It is appropriate to add a disintegrating agent, a lubricant, etc., and use it in the form of tablets, granules, powders, powders, capsules and the like.

The radiation injury inhibitor or food composition comprising the chlorella of the present invention as an active ingredient is preferably administered orally. The daily intake is preferably 3 g to 10 g, more preferably 3 g to 8 g, and still more preferably 4 g to 7 g.
Chlorella vulgaris E-25 powder used in the radiation injury suppression test was obtained by cultivating chlorella vulgaris E-25 in a culture tank, collecting the cells by centrifugation, drying and crushing them.

When this Chlorella vulgaris E-25 powder was administered to a cancer patient undergoing radiotherapy, a marked difference was observed in the side effect suppression effect compared to the target group that was not administered.
That is, the total effective rate with remarkable effect or effect was 21.1% in the non-administered group (effective), but had a (suppressing) effect, but the difference was about twice as high as 40% in the administration group. It was seen. Furthermore, among the effects observed, the remarkable effect was 0% in the subject group, but a significant difference was seen in 6 (17.1%) in the administration group. Effectiveness was not significantly different between the control group 21.1% and the administration group 22.9%.

From this, it was considered that Chlorella vulgaris E-25 powder has the effect of reducing the side effects of radiotherapy.
When using the chlorella concerning this invention as a drink or a foodstuff, the following forms can be considered.

(Tea drink)
A novel tea beverage can also be produced using the chlorella ground product or composition of the present invention. For example, an appropriate amount of the chlorella pulverized product or composition according to the present invention is added to the raw material (preferably 0.1 to 30% of the raw material is not limited to this, but the addition time is the time of hot water or hot water extraction, or the final (This is preferred, but not limited to this at the time of product blending.) By producing a tea beverage according to a production method well known to those skilled in the art, a tea beverage having a novel flavor can be produced. In other words, richness and horses can be strengthened. Moreover, a color can also be provided and the effect which can adjust the color of a product is also acquired. Here, tea drinks are, for example, Chinese tea such as barley tea, brown rice tea, green tea, oolong tea, dokudami tea, habu tea, matcha tea, gyokuro, kyoban tea, hoji tea, sencha sagagaon, gyokuro sagane , Sencha, powdered tea, Tochu tea, reduced fertilizer tea, rooibos tea, barley tea, new tea and mixed teas made by mixing multiple ingredients.

(Soft drink)
A soft drink having a new flavor can also be produced using the chlorella ground product or composition. For example, an appropriate amount of chlorella pulverized product or composition according to the present invention is added to the raw material (preferably 0.1 to 30% of the raw material is not limited to this, but the addition time may be any step of the production process, The final product blending is preferred, but not limited to this.) By producing a soft drink according to a production method well known to those skilled in the art, a soft drink having a novel flavor can be produced. It can add a rich fragrance, sweet scent, grain-like scent, and enhance richness and horses. Moreover, a color can also be provided and the effect which can adjust the color of a product is also acquired. Here, soft drinks include juices, carbonated drinks, malt drinks, lactic acid bacteria drinks, health drinks, sports drinks, amino acid drinks, vitamin drinks, etc., but are not limited thereto.

(Coffee drink)
It is also possible to produce a coffee beverage having a new flavor by using a chlorella ground product or a composition. For example, an appropriate amount of the chlorella pulverized product or composition according to the present invention is added to the raw material (preferably 0.1 to 10% of the raw material, but this is not the only case, the addition time may be any step of the production process, (Although it is preferable when the final product is blended, etc., it is not limited to this.) A coffee beverage having a novel flavor can be produced by producing a coffee beverage according to a production method well known to those skilled in the art. That is, richness, horses, etc. can be strengthened in coffee. Here, coffee beverages include, but are not limited to, coffee, coffee beverages, and soft drinks with coffee.

(Confectionery)
A confectionery having a new flavor can also be produced by using a chlorella ground product or a composition. For example, an appropriate amount of the chlorella pulverized product or composition according to the present invention is added to the raw material (from 0.1% to 100% of the raw material. The timing of addition may be any step of the manufacturing process, or added to the final product. However, the present invention is not limited to this.) A confectionery having a novel aroma can be produced by producing the confectionery according to a production method well known to those skilled in the art. You can strengthen the body and horses. Moreover, a color can also be provided and the effect which can adjust the color of a product is also acquired. Here, confectionery includes, but is not limited to, pon confectionery, cookies, biscuits, rice crackers, hail, rice crackers, rice cakes, gums, and snacks.

(Rice)
It is also possible to produce rice having a new flavor by using a chlorella pulverized product or a composition. For example, an appropriate amount of the chlorella pulverized product or composition according to the present invention is added to the raw material (0.1 to 30% of the raw material. The timing of addition may be any step in the manufacturing process, or added to the final product. However, the present invention is not limited to this.) A rice having a novel flavor can be produced by producing a rice according to a conventional method for producing a rice. You can strengthen the body and horses. Moreover, a color can also be provided and the effect which can adjust the color of a product is also acquired. Here, rice is white rice, brown rice, shelled rice-like food, baked rice, fried rice, cooked rice, barley rice, buckwheat rice, pot rice, red rice, bird rice, gomoku rice, matsutake rice, oyster rice, potato rice, Say crab rice, scallop rice, etc.

Production of Chlorella Powder Chlorella vulgaris E-25 was cultured in a sterile tank, and the cells were collected by centrifugation and then powdered by a spray drying method.
Measurement of Inhibitory Effect on Radiation Damage 74 tumor patients receiving radiation treatment were selected and randomly divided into 48 experimental groups and 26 control groups. The experimental plot is 30 males and 18 females, with an average age of 53.79 ± 15.33 years. The control plot is 20 males and 6 females with an average age of 56.20 ± 16.83. These patients were treated with radiation, and Chlorella E-25 powder was administered only to the experimental section to measure the effect.

In the experimental group, the patient was given radiation therapy and simultaneously received Chlorella E-25 powder. The dose is 1.5 grams 3 times a day.
For the control group, other drugs and blood products related to the adjuvant treatment were not used while receiving radiation treatment, and only the presence or absence of chlorella administration was different from the experimental group.
Participants were tested for blood, urine, liver, kidney and immune cell proteins before and after receiving radiation therapy to determine the effect of Chlorella E-25.

For the determination of the effect, three kinds of evaluations, a remarkable effect, an effect, and an invalidity, were performed.
Significant effect A White blood cells remain stable or elevated after radiation therapy. The number and classification of white blood cells are in the normal range.
B After radiation therapy, IgA, IgG and IgM should be within the normal range (IgA: 0.2-5g / L, IgG: 6-16g / L, IgM: 0.6-2g / L)
C After the radiotherapy, the platelet count and hemoglobin are within the normal range.
(Platelet count: 100-300 × 10 / L, hemoglobin: male 120-160 g / L, female 110-150 g / L)

Effective A After the radiotherapy, the decrease in white blood cell count does not exceed 30% and the white blood cell count and classification are within the normal range.
After radiotherapy, one or more of IgA, IgG, and IgM should not be lower than normal (IgA: 0.2-5g / L, IgG: 6-16g / L, IgM: 0.6-2g / L)
C After the radiotherapy, the decrease in platelet count and hemoglobin should be within the normal range without exceeding 30%.
(Platelet count: 100-300 × 10 / L, hemoglobin: male 120-160 g / L, female 110-150 g / L)
Invalid Valid standard not reached

Subjective indicators The following items were measured as subjective indicators.
It is considered invalid if the improvement value of subjective indicators such as loss of appetite, headache, nausea, vomiting, neglect and inability is ≦ 0.
In addition, some patients have multiple cancers, and patients with multiple cases were counted for each case.

As a result of measuring the effect according to the above index, the experimental group had more remarkable effects and effects than the control group (see Example 1, Table 2). Therefore, chlorella E-25 was considered to have an effect of reducing the side effects of radiation therapy.
The administration form of Chlorella E-25 may be a powder of chlorella cells, or may be in the form of an extract or a composition.

The patient group for measuring the administration effect of Chlorella vulgaris E-25 was performed according to the disease types and the number of cases in Table 1. The experiment was performed by randomly dividing the control group into 28 cases and the administration group as 55 cases.

As a result, the results shown in Table 2 were obtained. None of the patients showed significant effects in the control group, but 6 (17.1%) showed significant effects in the experimental group. Effectiveness was not significantly different between the control group 21.1% and the experimental group 22.9%. No effect (invalid) was found in the experimental group, which was 79.0% in the control group and 60.0% in the experimental group.

  The number of WBC (white blood cells) and platelets were significantly decreased in the control group compared to before the experiment, but no significant decrease was observed in the experimental group. In addition, the WBC (white blood cell) count and platelets were significantly higher in the experimental group than in the control group.

Regarding the amount of immunity protein, IgM was significantly higher in the experimental group than in the control group after radiotherapy. Regarding IgA and IgG, the experimental group showed slightly higher values after radiotherapy, but no significant difference was observed.

Regarding SGPT (serum glutamate pyruvate transaminase), BUN (blood urea nitrogen) and ALB (albumin), no significant difference was observed before and after radiotherapy.

Regarding subjective symptom improvement, the control group showed an approximately 2-fold increase in appetite loss and nausea before and after treatment, but the experimental group showed almost no difference before and after treatment. From this, it is considered that the experimental group has an inhibitory effect on appetite loss and nausea.

  The radiation injury inhibitor of the present invention can be used in the pharmaceutical industry and the food industry.

Claims (2)

  A radiation injury inhibitor comprising chlorella vulgaris powder as an active ingredient.   Chlorella vulgaris is E-25 strain | stump | stock, The radiation disorder inhibitor of Claim 1 characterized by the above-mentioned.