JP2014110812A - Composition for proliferating lactobacillus, medium for lactobacillus and method for culturing lactobacillus - Google Patents
Composition for proliferating lactobacillus, medium for lactobacillus and method for culturing lactobacillus Download PDFInfo
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本発明は、乳酸菌を増殖させることができる乳酸菌増殖用組成物、乳酸菌用培地及び乳酸菌の培養方法に関する。 The present invention relates to a composition for growing lactic acid bacteria capable of growing lactic acid bacteria, a medium for lactic acid bacteria, and a method for culturing lactic acid bacteria.
乳酸菌は、グルコース等の糖類から多量の乳酸を生成する細菌の総称であり、醤油、清酒、味噌等に見出される他、乳製品、穀類、腸等にも分布している。その培養には、発酵乳、乳酸菌飲料、チーズ等の製造のために獣乳を含む培地を使用することが多いが、乳酸菌の栄養要求性が厳格であるため、増殖に適さない菌株が多いという問題がある。 Lactic acid bacteria are a general term for bacteria that produce a large amount of lactic acid from sugars such as glucose, and are also found in soy sauce, sake, miso, etc., and are also distributed in dairy products, cereals, intestines, and the like. The culture often uses a medium containing animal milk for the production of fermented milk, lactic acid bacteria beverages, cheese, etc., but there are many strains that are not suitable for growth due to the strict nutritional requirements of lactic acid bacteria. There's a problem.
そこで、乳酸菌を増殖させるための物質として、培地中に、酵母エキス、麦芽エキス、ペプトン、アミノ酸等を添加することが知られているが（例えば、特許文献１参照）、高価な上に培養条件が制限され、安価かつ簡便に、乳酸菌を増殖させることが難しいという問題がある。 Therefore, it is known to add yeast extract, malt extract, peptone, amino acid, etc. to the medium as a substance for growing lactic acid bacteria (see, for example, Patent Document 1), but the culture conditions are expensive. However, there is a problem that it is difficult to grow lactic acid bacteria inexpensively and easily.
したがって、安価かつ簡便に、乳酸菌を増殖させることができる乳酸菌増殖用組成物、並びに、乳酸菌の培養に好適な乳酸菌用培地及び乳酸菌の培養方法の速やかな提供が強く求められているのが現状である。 Therefore, at present, there is a strong demand for prompt provision of a composition for growing lactic acid bacteria that can grow lactic acid bacteria inexpensively and easily, a medium for lactic acid bacteria suitable for culturing lactic acid bacteria, and a method for culturing lactic acid bacteria. is there.
本発明は、前記従来における諸問題を解決し、以下の目的を達成することを課題とする。即ち、本発明は、安価かつ簡便に、乳酸菌を増殖させることができる乳酸菌増殖用組成物、並びに、乳酸菌の培養に好適な乳酸菌用培地及び乳酸菌の培養方法を提供することを目的とする。 An object of the present invention is to solve the conventional problems and achieve the following objects. That is, an object of the present invention is to provide a composition for growing lactic acid bacteria capable of growing lactic acid bacteria inexpensively and easily, a medium for lactic acid bacteria suitable for culturing lactic acid bacteria, and a method for culturing lactic acid bacteria.
前記課題を解決するため、本発明者らは鋭意検討した結果、以下のような知見を得た。即ち、大麦若葉を含有する乳酸菌増殖用組成物を用いて乳酸菌を培養することにより、安価かつ簡便に、乳酸菌を増殖させることができることを見出した。 In order to solve the above-mentioned problems, the present inventors have made extensive studies and as a result, obtained the following findings. That is, it has been found that lactic acid bacteria can be grown inexpensively and easily by culturing lactic acid bacteria using a composition for growing lactic acid bacteria containing young barley leaves.
本発明は、前記知見に基づくものであり、前記課題を解決するための手段としては、以下の通りである。即ち、
＜１＞ 大麦若葉を含有することを特徴とする乳酸菌増殖用組成物である。
＜２＞ 大麦若葉が、粉末状である前記＜１＞に記載の乳酸菌増殖用組成物である。
＜３＞ 大麦若葉の水分含有量が、５質量％以下である前記＜１＞から＜２＞のいずれかに記載の乳酸菌増殖用組成物である。
＜４＞ 大麦若葉の乳酸菌増殖用組成物における含有量が、０．００２質量％以上である前記＜１＞から＜３＞のいずれかに記載の乳酸菌増殖用組成物である。
＜５＞ 更に、オリゴ糖及び水溶性食物繊維の少なくともいずれかを含有する前記＜１＞から＜４＞のいずれかに記載の乳酸菌増殖用組成物である。
＜６＞ 更に、乳酸菌を含有する前記＜１＞から＜５＞のいずれかに記載の乳酸菌増殖用組成物である。
＜７＞ 乳酸菌が、ストレプトコッカス・フェカリス（Ｓｔｒｅｐｔｏｃｏｃｃｕｓ ｆａｅｃａｌｉｓ）である前記＜１＞から＜６＞のいずれかに記載の乳酸菌増殖用組成物である。
＜８＞ 前記＜１＞から＜７＞のいずれかに記載の乳酸菌増殖用組成物を含有することを特徴とする乳酸菌用培地である。
＜９＞ 前記＜８＞に記載の乳酸菌用培地を用いて乳酸菌を培養することを特徴とする乳酸菌の培養方法である。
The present invention is based on the above knowledge, and means for solving the above problems are as follows. That is,
<1> A composition for growing lactic acid bacteria, comprising young barley leaves.
<2> The composition for growing lactic acid bacteria according to <1>, wherein the young barley leaves are in a powder form.
<3> The composition for growing lactic acid bacteria according to any one of <1> to <2>, wherein the water content of the barley young leaves is 5% by mass or less.
<4> The composition for growing lactic acid bacteria according to any one of <1> to <3>, wherein the content of the barley young leaves in the composition for growing lactic acid bacteria is 0.002% by mass or more.
<5> The composition for growing lactic acid bacteria according to any one of <1> to <4>, further comprising at least one of an oligosaccharide and a water-soluble dietary fiber.
<6> The composition for growing lactic acid bacteria according to any one of <1> to <5>, further comprising lactic acid bacteria.
<7> The composition for growing lactic acid bacteria according to any one of <1> to <6>, wherein the lactic acid bacteria is Streptococcus faecalis.
<8> A culture medium for lactic acid bacteria comprising the composition for growing lactic acid bacteria according to any one of <1> to <7>.
<9> A method for cultivating lactic acid bacteria, comprising culturing lactic acid bacteria using the medium for lactic acid bacteria according to <8>.
本発明によると、従来における諸問題を解決することができ、安価かつ簡便に、乳酸菌を増殖することができる乳酸菌増殖用組成物、並びに、乳酸菌の培養に好適な乳酸菌用培地及び乳酸菌の培養方法を提供することができる。 INDUSTRIAL APPLICABILITY According to the present invention, various conventional problems can be solved, and a composition for growing lactic acid bacteria capable of growing lactic acid bacteria inexpensively and easily, a medium for lactic acid bacteria suitable for culturing lactic acid bacteria, and a method for culturing lactic acid bacteria Can be provided.
（乳酸菌増殖用組成物）
本発明の乳酸菌増殖用組成物は、大麦若葉を含有し、好ましくはオリゴ糖、水溶性食物繊維、及び乳酸菌を含有し、更に必要に応じてその他の成分を含有する。
(Composition for lactic acid bacteria growth)
The composition for growing lactic acid bacteria of the present invention contains barley young leaves, preferably contains oligosaccharides, water-soluble dietary fiber, and lactic acid bacteria, and further contains other components as necessary.
＜大麦若葉＞
前記大麦若葉は、オオムギ（Ｈｏｒｄｅｕｍ ｖｕｌｇａｒｅ Ｌ．）の若葉であり、通常入手可能なものであれば、特に制限はなく、目的に応じて適宜選択することができる。
また、前記大麦若葉は、栄養価が高く、ビタミン、ミネラル、葉緑素などに富むことから、近年注目されている健康食品素材である。
<Young barley leaves>
The barley young leaves are young leaves of barley (Hordeum vulgare L.), and are not particularly limited as long as they are usually available, and can be appropriately selected according to the purpose.
In addition, the barley young leaves are high in nutritional value and rich in vitamins, minerals, chlorophyll, and the like, and thus are health food materials that have attracted attention in recent years.
前記大麦の品種としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、二条大麦、四条大麦、六条大麦、裸麦などが挙げられる。 The variety of barley is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include Nijo barley, Shijo barley, Rojo barley, and bare barley.
前記大麦若葉の使用形態としては、特に制限はなく、目的に応じて適宜選択することができるが、粗く裁断したチップ状の大麦若葉と比較して、乳酸菌との接触面積を増やすことができる点、並びにペースト状や液体状の大麦若葉と比較して、雑菌繁殖抑制、輸送及び保管コスト抑制の点で、粉末状の大麦若葉が好ましい。
前記大麦若葉の水分含有量としては、特に制限はなく、目的に応じて適宜選択することができるが、雑菌繁殖抑制や大麦若葉の変色（退色）抑制の点で、５質量％以下が好ましい。
The use form of the barley young leaves is not particularly limited and can be appropriately selected according to the purpose, but the contact area with lactic acid bacteria can be increased as compared to the roughly cut chip-shaped barley young leaves. Compared to pasty and liquid barley young leaves, powdered barley young leaves are preferable in terms of suppression of miscellaneous bacteria growth, transportation and storage costs.
There is no restriction | limiting in particular as water content of the said barley young leaf, Although it can select suitably according to the objective, 5 mass% or less is preferable at the point of miscellaneous bacteria reproduction suppression and the barley young leaf discoloration (fading) suppression.
前記乳酸菌増殖用組成物における大麦若葉粉末の含有量としては、特に制限はなく、目的に応じて適宜選択することができるが、下限値としては、０．００２質量％以上が好ましく、０．００３質量％以上がより好ましく、０．０１質量％以上が更に好ましく、０．１質量％以上が特に好ましく、上限値としては、９０質量％以下が特に好ましい。前記含有量が、０．００２質量％未満であると、乳酸菌が増殖されにくいことがある。 There is no restriction | limiting in particular as content of the barley young leaf powder in the said composition for lactic acid bacteria growth, Although it can select suitably according to the objective, As a lower limit, 0.002 mass% or more is preferable, 0.003 More preferably, it is more preferably 0.01% by weight or more, particularly preferably 0.1% by weight or more, and particularly preferably 90% by weight or less. If the content is less than 0.002% by mass, lactic acid bacteria may be difficult to grow.
−大麦若葉の粉末処理−
前記大麦若葉の粉末処理としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、前処理、ブランチング処理、乾燥処理、及び破砕処理をこの順に行う粉末処理などが挙げられ、更に必要に応じてその他の処理を行ってもよく、特許第３４２８９５６号公報、特許第３２７７１８１号公報等に記載の粉末処理に基づいて行ってもよい。
-Powder processing of young barley leaves-
There is no restriction | limiting in particular as powder processing of the said barley young leaf, According to the objective, it can select suitably, For example, the powder processing etc. which perform a pre-processing, a blanching process, a drying process, and a crushing process in this order are mentioned. Further, other treatments may be performed as necessary, and may be performed based on the powder treatment described in Japanese Patent No. 3428956, Japanese Patent No. 3277181, and the like.
−−前処理−−
前記前処理としては、特に制限はなく、目的に応じて適宜選択することができるが、成熟期前、即ち分げつ開始期から出穂開始前期（背丈が２０ｃｍ〜４０ｃｍ程度）に収穫された大麦若葉を水（好ましくは２５℃以下の冷水）で洗浄し、泥などを洗い落とし、水気を切った後、適当な長さ（例えば、５ｃｍ〜１０ｃｍ）に切断することが好ましい。なお、収穫後、直ちに処理されることが好ましく、処理までに時間を要する場合、緑葉の変質を防ぐために低温貯蔵などの当業者が通常用いる貯蔵手段により貯蔵されることが好ましい。
--Pre-treatment--
The pretreatment is not particularly limited and may be appropriately selected according to the purpose. Barley harvested before the maturity period, that is, from the beginning of tillering to the early stage of heading (about 20 to 40 cm in height). It is preferable to wash the young leaves with water (preferably cold water of 25 ° C. or less), wash off mud, etc., drain the water, and then cut it into an appropriate length (for example, 5 cm to 10 cm). In addition, it is preferable to process immediately after harvesting, and when it takes time to process, it is preferable to store by the storage means normally used by those skilled in the art, such as low-temperature storage, in order to prevent deterioration of green leaves.
−−ブランチング処理−−
前記ブランチング処理は、緑葉の緑色の褪色や栄養成分の変質に関与する酵素を失活させるために行う。
前記ブランチング処理としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、熱水処理、蒸熱処理などが挙げられる。
前記ブランチング処理の温度及び時間としては、処理する緑葉の量及び熱水のｐＨに応じて適宜決定すればよい。
前記ブランチング処理された大麦若葉は、緑色及び風味を維持する上で、直ちに冷却されることが好ましい。前記冷却としては、例えば、冷却水中への浸漬処理、冷風を当てた急冷処理などの当業者が通常用いる処理であればよい。前記冷却水としては、３０℃以下の水が好ましく、２０℃以下の水がより好ましい。前記冷却水の温度が低いほど、緑葉の緑色が映えるようになり、見た目が美しくなる。前記浸漬時間としては、緑葉の処理量に応じた任意の時間であればよい。
前記ブランチング処理を行った場合は、冷却後、乾燥にさきがけて、緑葉からある程度の水を切る脱水を行うことが好ましい。前記脱水は、遠心分離などの脱水装置を用いて行われる。前記遠心分離による脱水の場合、緑葉がからまってしまう場合は、緑葉をほぐした後、乾燥処理に移行することが好ましい。乾燥前に脱水することにより、乾燥時のむらや焦げつきをなくすことができる。
--Branching process--
The blanching treatment is performed to inactivate enzymes involved in the green discoloration of green leaves and alteration of nutrient components.
There is no restriction | limiting in particular as said blanching process, According to the objective, it can select suitably, For example, a hot water process, steaming, etc. are mentioned.
What is necessary is just to determine suitably as the temperature and time of the said blanching process according to the quantity of the green leaf to process, and the pH of hot water.
The blanched young barley leaves are preferably cooled immediately to maintain the green color and flavor. As said cooling, what is necessary is just the process normally used by those skilled in the art, such as the immersion process in cooling water, the rapid cooling process which applied the cold wind, etc. As said cooling water, 30 degrees C or less water is preferable, and 20 degrees C or less water is more preferable. The lower the temperature of the cooling water, the more green the green leaves shine and the more beautiful it looks. As said immersion time, what is necessary is just arbitrary time according to the processing amount of the green leaf.
When the blanching treatment is performed, it is preferable to perform dehydration after cooling to remove some water from the green leaves before drying. The dehydration is performed using a dehydration apparatus such as a centrifugal separator. In the case of dehydration by centrifugation, if green leaves get tangled, it is preferable to loosen the green leaves and then move to a drying process. By dehydrating before drying, unevenness and scorching during drying can be eliminated.
−−乾燥処理−−
前記乾燥処理としては、特に制限はなく、目的に応じて適宜選択することができるが、前記ブランチング処理された大麦若葉の水分含量を、１０％以下となるよう乾燥する処理が好ましく、５％以下となるよう乾燥する処理がより好ましい。
前記乾燥処理における乾燥方法としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、熱風乾燥、高圧蒸気乾燥、電磁波乾燥、凍結乾燥等により乾燥する方法などが挙げられる。
前記熱風乾燥における乾燥温度及び乾燥時間としては、前記大麦若葉が変色しない条件であれば、特に制限はなく、目的に応じて適宜選択することができるが、７０℃〜１４０℃が好ましく、８０℃〜１３０℃がより好ましい。
--- Drying process--
There is no restriction | limiting in particular as said drying process, Although it can select suitably according to the objective, The process which dries so that the water content of the said barley young leaf blanched may be 10% or less is preferable, and 5% A treatment of drying so as to be the following is more preferable.
There is no restriction | limiting in particular as a drying method in the said drying process, According to the objective, it can select suitably, For example, the method etc. which dry by hot air drying, high pressure steam drying, electromagnetic wave drying, freeze drying, etc. are mentioned.
The drying temperature and drying time in the hot-air drying are not particularly limited as long as the barley young leaves are not discolored, and can be appropriately selected according to the purpose, but preferably 70 ° C to 140 ° C, 80 ° C. -130 degreeC is more preferable.
−−破砕処理−−
前記破砕処理としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、前記乾燥処理された大麦若葉を、クラッシャー、ミル、ブレンダー、石臼等を用いて粉砕する処理などが挙げられる。前記粉砕処理後の大麦若葉を必要に応じて、例えば、３０メッシュ〜２５０メッシュの篩にかけて通過したものを大麦若葉粉末として用いることができる。
--Crushing process--
The crushing treatment is not particularly limited and can be appropriately selected depending on the purpose. Examples thereof include a treatment for crushing the dried barley leaves using a crusher, a mill, a blender, a stone mill, and the like. It is done. If necessary, the barley young leaves after the pulverization treatment can be used as barley young leaf powder after passing through a sieve of 30 mesh to 250 mesh, for example.
−−その他の処理−−
前記その他の処理としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、気流、高圧蒸気、加熱等による殺菌処理などが挙げられる。
-Other processing-
There is no restriction | limiting in particular as said other process, According to the objective, it can select suitably, For example, the sterilization process by an airflow, a high pressure steam, a heating, etc. are mentioned.
−乳酸菌増殖用組成物の調製方法−
前記乳酸菌増殖用組成物の調製方法としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、（１）無機塩類を含む溶媒に前記大麦若葉粉末を溶解して調製する方法、（２）無機塩類を含む溶媒に前記大麦若葉粉末を溶解した後、更に前記オリゴ糖、前記水溶性食物繊維、前記乳酸菌、及び前記その他の成分を溶解させて調製する方法などが挙げられる。前記無機塩類を含む溶媒としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、生理食塩水、リン酸緩衝液、リン酸緩衝生理食塩水などが挙げられるが、これらの中でも、リン酸緩衝生理食塩水（ＰＢＳ）が好ましい。
-Preparation method of lactic acid bacteria growth composition-
The method for preparing the composition for growing lactic acid bacteria is not particularly limited and may be appropriately selected depending on the intended purpose. For example, (1) a method for preparing by dissolving the barley young leaf powder in a solvent containing inorganic salts (2) After dissolving the barley young leaf powder in a solvent containing inorganic salts, the oligosaccharide, the water-soluble dietary fiber, the lactic acid bacteria, and the other components are further prepared. The solvent containing the inorganic salt is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include physiological saline, phosphate buffer, phosphate buffered saline, and the like. Among these, phosphate buffered saline (PBS) is preferable.
＜オリゴ糖＞
前記オリゴ糖を本発明の乳酸菌増殖用組成物に含有させることにより、より安価かつ簡便に乳酸菌を増殖させることができる。
前記オリゴ糖としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、スクロース、ラクトース、トレハロース、マルトース、フラクトオリゴ糖、ガラクトオリゴ糖、イソマルトオリゴ糖、キシロオリゴ糖、シクロデキストリンなどが挙げられる。これらは、１種単独で使用してもよいし、２種以上を併用してもよい。
前記オリゴ糖の前記乳酸菌増殖用組成物における含有量としては、特に制限はなく、目的に応じて適宜選択することができるが、０．１質量％〜３０質量％が好ましく、１質量％〜１０質量％がより好ましい。
<Oligosaccharide>
By including the oligosaccharide in the composition for growing lactic acid bacteria of the present invention, the lactic acid bacteria can be grown more inexpensively and easily.
The oligosaccharide is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include sucrose, lactose, trehalose, maltose, fructooligosaccharide, galactooligosaccharide, isomaltoligosaccharide, xylooligosaccharide, and cyclodextrin. It is done. These may be used individually by 1 type and may use 2 or more types together.
There is no restriction | limiting in particular as content in the said composition for lactic acid bacteria growth of the said oligosaccharide, Although it can select suitably according to the objective, 0.1 mass%-30 mass% are preferable, and 1 mass%-10 mass. The mass% is more preferable.
＜水溶性食物繊維＞
前記水溶性食物繊維を本発明の乳酸菌増殖用組成物に含有させることにより、より安価かつ簡便に乳酸菌を増殖させることができる。
前記水溶性食物繊維としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、難消化性デキストリン、ペクチン、アルギン酸、グアーガム、グアーガム加水分解物、グルコマンナン、ガラクトマンナン、ポリデキストロース、カラギーナンなどが挙げられる。これらは、１種単独で使用してもよいし、２種以上を併用してもよい。
前記水溶性食物繊維の前記乳酸菌増殖用組成物における含有量としては、特に制限はなく、目的に応じて適宜選択することができるが、１質量％〜５０質量％が好ましく、５質量％〜４０質量％がより好ましい。
<Water-soluble dietary fiber>
By including the water-soluble dietary fiber in the composition for growing lactic acid bacteria of the present invention, the lactic acid bacteria can be grown more inexpensively and easily.
The water-soluble dietary fiber is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include indigestible dextrin, pectin, alginic acid, guar gum, guar gum hydrolysate, glucomannan, galactomannan, polydextrose And carrageenan. These may be used individually by 1 type and may use 2 or more types together.
The content of the water-soluble dietary fiber in the composition for growing lactic acid bacteria is not particularly limited and may be appropriately selected depending on the intended purpose, but is preferably 1% by mass to 50% by mass, and 5% by mass to 40%. The mass% is more preferable.
＜乳酸菌＞
前記乳酸菌を予め本発明の乳酸菌増殖用組成物に含有させることにより、より安価かつ簡便に前記乳酸菌を増殖させることができる。
前記乳酸菌としては、糖類から多量の乳酸を生成する細菌であれば、特に制限はなく、目的に応じて適宜選択することができ、例えば、ラクトバチルス・アシドフィルス（Ｌａｃｔｏｂａｃｉｌｌｕｓ ａｃｉｄｏｐｈｉｌｕｓ）、ラクトバチルス・カゼイ（Ｌａｃｔｏｂａｃｉｌｌｕｓ ｃａｓｅｉ）、ストレプトコッカス・フェカリス（Ｓｔｒｅｐｔｏｃｏｃｃｕｓ ｆａｅｃａｌｉｓ）（エンテロコッカス・フェカリス（Ｅｎｔｅｒｏｃｏｃｃｕｓ ｆａｅｃａｌｉｓ）と称されることがある）、ストレプトコッカス・サーモフィラス（Ｓｔｒｅｐｔｏｃｏｃｃｕｓ ｔｈｅｒｍｏｐｈｉｌｕｓ）、バチルス・コアギュランス（Ｂａｃｉｌｌｕｓ ｃｏａｇｕｌｌａｎｓ）などが挙げられる。これらは、１種単独で使用してもよいし、２種以上を併用してもよい。
前記乳酸菌の性質としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、耐熱性、耐酸性、耐糖性、耐塩性、有胞子性などが挙げられる。
前記乳酸菌の入手方法としては、特に制限はなく、目的に応じて適宜選択することができ、例えば、ヨーグルトや野菜等の食品から単離された乳酸菌や市販品を用いてもよい。
前記乳酸菌の前記乳酸菌増殖用組成物における含有量としては、特に制限はなく、目的に応じて適宜選択することができるが、０．０１質量％〜１０質量が好ましく、０．１質量％〜５質量％がより好ましい。
<Lactic acid bacteria>
By previously containing the lactic acid bacteria in the composition for growing lactic acid bacteria of the present invention, the lactic acid bacteria can be grown more inexpensively and easily.
The lactic acid bacterium is not particularly limited as long as it is a bacterium that produces a large amount of lactic acid from saccharides, and can be appropriately selected according to the purpose. For example, Lactobacillus acidophilus, Lactobacillus casei ( Lactobacillus casei), Streptococcus faecalis (sometimes referred to as Enterococcus faecalis), Streptococcus thermophilus (L), Streptococcus thermophyllus (Streptococcus thermos) These may be used individually by 1 type and may use 2 or more types together.
There is no restriction | limiting in particular as a property of the said lactic acid bacteria, According to the objective, it can select suitably, For example, heat resistance, acid resistance, sugar resistance, salt resistance, spore resistance etc. are mentioned.
There is no restriction | limiting in particular as the acquisition method of the said lactic acid bacteria, According to the objective, it can select suitably, For example, you may use the lactic acid bacteria isolated from foodstuffs, such as yogurt and vegetables, or a commercial item.
There is no restriction | limiting in particular as content in the said composition for lactic acid bacteria growth of the said lactic acid bacteria, Although it can select suitably according to the objective, 0.01 mass%-10 mass are preferable, 0.1 mass%-5 The mass% is more preferable.
＜その他の成分＞
前記その他の成分としては、本発明の効果を損なわない範囲内であれば、特に制限はなく、目的に応じて適宜選択することができ、例えば、賦形剤、増量剤、結合剤、増粘剤、乳化剤、着色料、香料などが挙げられる。
<Other ingredients>
The other components are not particularly limited as long as they do not impair the effects of the present invention, and can be appropriately selected according to the purpose. For example, excipients, extenders, binders, thickeners Agents, emulsifiers, colorants, fragrances and the like.
＜用途＞
本発明の乳酸菌増殖用組成物は、安価かつ簡便に、乳酸菌を増殖させることができるため、特に以下に示す、乳酸菌用培地及び乳酸菌の培養方法において好適に用いることができる。
<Application>
Since the composition for lactic acid bacteria growth of this invention can proliferate lactic acid bacteria cheaply and simply, it can be conveniently used especially in the culture medium of lactic acid bacteria and the culture | cultivation method of lactic acid bacteria shown below.
（乳酸菌用培地及び乳酸菌の培養方法）
前記乳酸菌用培地は、前記乳酸菌増殖用組成物を含有してなり、更に必要に応じて乳酸菌の生育に好適な成分を含有してなる。
前記乳酸菌の培養方法は、前記乳酸菌用培地を用いて乳酸菌を培養する方法である。
(Medium for lactic acid bacteria and culture method of lactic acid bacteria)
The medium for lactic acid bacteria contains the composition for growing lactic acid bacteria, and further contains components suitable for the growth of lactic acid bacteria as necessary.
The method for culturing lactic acid bacteria is a method for culturing lactic acid bacteria using the medium for lactic acid bacteria.
＜乳酸菌＞
本発明の乳酸菌用培地により増殖される乳酸菌としては、糖類から多量の乳酸を生成する細菌であれば、特に制限はなく、目的に応じて適宜選択することができ、上述の乳酸菌と同様の菌などが挙げられる。
<Lactic acid bacteria>
The lactic acid bacteria grown on the medium for lactic acid bacteria of the present invention are not particularly limited as long as they produce a large amount of lactic acid from saccharides, and can be appropriately selected according to the purpose. Etc.
＜培地成分＞
前記乳酸菌用培地における培地成分としては、前記乳酸菌増殖用組成物を含有するものであれば、特に制限はなく、通常乳酸菌に使用される培地の成分の中から、目的に応じて適宜選択することができ、例えば、グルコース、オリゴ糖等の炭素源；ポリペプトン、酵母エキス、カゼイン等の窒素源；リン酸ナトリウム、リン酸カリウム、塩化ナトリウム、炭酸カルシウム、硫酸アンモニウム等の無機塩類などの乳酸菌の生育に好適な成分などが挙げられるが、前記リン酸緩衝生理食塩水と前記大麦若葉粉末を含有する乳酸菌増殖用組成物とからなる液体培地が、好適に乳酸菌を増殖させることができる点で好ましい。また、前記乳酸菌増殖用組成物を含む液体培地を、前培養において用いてもよく、本培養において用いてもよい。
<Media components>
The medium component in the medium for lactic acid bacteria is not particularly limited as long as it contains the composition for growing lactic acid bacteria, and is appropriately selected from the components of the medium normally used for lactic acid bacteria according to the purpose. For example, for the growth of lactic acid bacteria such as carbon sources such as glucose and oligosaccharides; nitrogen sources such as polypeptone, yeast extract and casein; and inorganic salts such as sodium phosphate, potassium phosphate, sodium chloride, calcium carbonate and ammonium sulfate. Although a suitable component etc. are mentioned, the liquid culture medium which consists of the said phosphate buffered saline and the composition for lactic acid bacteria proliferation containing the said barley young leaf powder is preferable at the point which can grow a lactic acid bacterium suitably. Moreover, the liquid medium containing the said composition for lactic acid bacteria growth may be used in a preculture, and may be used in a main culture.
前記乳酸菌用培地における大麦若葉粉末の含有量としては、特に制限はなく、目的に応じて適宜選択することができるが、下限値としては、０．００２質量％以上が好ましく、０．００３質量％以上がより好ましく、０．０１質量％以上が更に好ましく、０．１質量％以上が特に好ましく、上限値としては、９０質量％以下が特に好ましい。前記含有量が、０．００２質量％未満であると、乳酸菌が増殖されにくいことがある。 There is no restriction | limiting in particular as content of the barley young leaf powder in the said culture medium for lactic acid bacteria, Although it can select suitably according to the objective, As a lower limit, 0.002 mass% or more is preferable, 0.003 mass% The above is more preferable, 0.01% by mass or more is further preferable, 0.1% by mass or more is particularly preferable, and the upper limit is particularly preferably 90% by mass or less. If the content is less than 0.002% by mass, lactic acid bacteria may be difficult to grow.
前記乳酸菌用培地における滅菌条件としては、前記大麦若葉に変質が生じず、乳酸菌に用いることができる培地を滅菌できる条件であれば、特に制限はなく、目的に応じて適宜選択することができるが、１１５℃〜１２６℃、１５分間〜３０分間で高圧蒸気滅菌することが好ましい。 The sterilization conditions in the medium for lactic acid bacteria are not particularly limited as long as the medium can be used for lactic acid bacteria without deterioration in the barley young leaves, and can be appropriately selected according to the purpose. It is preferable to perform autoclaving at 115 ° C. to 126 ° C. for 15 to 30 minutes.
＜培養条件＞
前記乳酸菌の培養条件（培地中のｐＨ、溶存酸素、培養温度、及び培養時間等）としては、通常乳酸菌に使用される培養条件であれば、特に制限はなく、目的に応じて適宜選択することができ、例えば、乳酸菌の科学と技術（乳酸菌研究集談会 編）等に記載の培養条件などが挙げられる。
<Culture conditions>
The culture conditions (pH in medium, dissolved oxygen, culture temperature, culture time, etc.) of the lactic acid bacteria are not particularly limited as long as they are culture conditions normally used for lactic acid bacteria, and should be appropriately selected according to the purpose. Examples include culturing conditions described in the science and technology of lactic acid bacteria (edited by lactic acid bacteria research conference).
＜効果＞
本発明の乳酸菌増殖用組成物、乳酸菌用培地及び乳酸菌の培養方法を用いることにより、安価かつ簡便に、乳酸菌の増殖を行うことができる。
<Effect>
By using the composition for growing lactic acid bacteria, the medium for lactic acid bacteria and the method for culturing lactic acid bacteria of the present invention, the lactic acid bacteria can be propagated inexpensively and easily.
以下に本発明の実施例を説明するが、本発明は、これらの実施例に何ら限定されるものではない。 Examples of the present invention will be described below, but the present invention is not limited to these examples.
（製造例１）
−大麦若葉粉末１の製造−
原料として、背丈が約３０ｃｍで刈り取った二条大麦（出穂開始前）の［大麦若葉１］を用いた。これを水洗いし、付着した泥などを除去し、５ｃｍ〜１０ｃｍ程度の大きさに切断して前処理を行った。この大麦若葉を、送帯型蒸機を用いて、ブランチング槽で９０℃〜１００℃にて９０秒間〜１２０秒間、１回のみブランチング処理を行い、その後、冷水により冷却を行った。次いで、得られる大麦若葉を、水分含量が５質量％以下となるように、乾燥機を用いて、２０分間〜５０分間、８０℃〜１３０℃の温風にて乾燥処理を行った。乾燥処理後に、大麦若葉を約５ｍｍの大きさに切断し、殺菌処理を行った。得られた大麦若葉を、２００メッシュ区分を９０％以上が通過するように気流式粉砕機（ジェットミル）を用いて粉砕処理を行い、［大麦若葉粉末１］を得た。
(Production Example 1)
-Manufacture of barley young leaf powder 1-
As a raw material, [Barley young leaf 1] of Nijo barley (before heading) that was cut to a height of about 30 cm was used. This was washed with water, adhering mud and the like were removed, and pretreated by cutting to a size of about 5 cm to 10 cm. This barley young leaf was subjected to a blanching treatment only once in a blanching tank at 90 to 100 ° C. for 90 seconds to 120 seconds using a band-type steamer, and then cooled with cold water. Next, the obtained barley young leaves were subjected to a drying treatment with hot air at 80 ° C. to 130 ° C. for 20 minutes to 50 minutes using a dryer so that the water content was 5% by mass or less. After the drying treatment, the barley young leaves were cut into a size of about 5 mm and sterilized. The obtained barley young leaves were pulverized using an air flow type pulverizer (jet mill) so that 90% or more of the 200 mesh sections passed through to obtain [Barley young leaves powder 1].
（製造例２）
−大麦若葉粉末２の製造−
製造例１で使用した原料である［大麦若葉１］とは別ロット（別ロットとは、製造時期の異なることを意味する。以下同じ。）の二条大麦（出穂開始前）の［大麦若葉２］に変更したこと以外は、製造例１と同様にして、［大麦若葉粉末２］を得た。
(Production Example 2)
-Manufacture of barley young leaf powder 2-
[Barley young leaf 2] of Nijo barley (before heading start) of a different lot (separate lot means different production time. Same applies hereinafter) from [barley young leaf 1] which is the raw material used in Production Example 1. ] [Barley young leaf powder 2] was obtained in the same manner as in Production Example 1, except that the change was changed to
（製造例３）
−大麦若葉粉末３の製造−
製造例１〜２で使用した原料である［大麦若葉１］〜［大麦若葉２］とは別ロットの二条大麦（出穂開始前）の［大麦若葉３］に変更したこと以外は、製造例１と同様にして、［大麦若葉粉末３］を得た。
(Production Example 3)
-Production of barley young leaf powder 3-
Production Example 1 except that [Barley Wakaba 1] to [Barley Wakaba 2], which are raw materials used in Production Examples 1 and 2, were changed to [Barley Wakaba 3] of Nijo Barley (before heading) in a different lot. In the same manner, [Barley young leaf powder 3] was obtained.
（製造例４）
−大麦若葉粉末４の製造−
製造例１〜３で使用した原料である［大麦若葉１］〜［大麦若葉３］とは別ロットの二条大麦（出穂開始前）の［大麦若葉４］に変更したこと以外は、製造例１と同様にして、［大麦若葉粉末４］を得た。
(Production Example 4)
-Manufacture of barley young leaf powder 4-
Production Example 1 except that [barley young leaf 1] to [barley young leaf 3], which are the raw materials used in production examples 1 to 3, were changed to [barley young leaf 4] in Nijo barley (before heading) in a different lot. In the same manner, [Barley young leaf powder 4] was obtained.
（試験例１：乳酸菌増殖試験１）
試験例１では、大麦若葉による乳酸菌の増殖を目視にて確認した。
(Test Example 1: Lactic acid bacteria growth test 1)
In Test Example 1, growth of lactic acid bacteria by barley young leaves was confirmed visually.
（実施例１）
＜乳酸菌増殖試験（大麦若葉＋ＰＢＳ）＞
−乳酸菌増殖用組成物の調製方法−
［大麦若葉粉末１］０．００１ｇをリン酸緩衝生理食塩水（ＰＢＳ）１０ｍＬ中に添加して０．０１質量％乳酸菌増殖用組成物を調製した。これを３倍希釈して（０．０１質量％乳酸菌増殖用組成物３ｍＬに、ＰＢＳ６ｍＬを加えて）、０．００３質量％乳酸菌増殖用組成物（９ｍＬ）とした。これをコニカルチューブに分注して、１２１℃、２０分間オートクレーブを行った。
Example 1
<Lactic acid bacteria growth test (barley young leaves + PBS)>
-Preparation method of lactic acid bacteria growth composition-
[Barley young leaf powder 1] 0.001 g was added to 10 mL of phosphate buffered saline (PBS) to prepare a composition for the growth of 0.01% by mass lactic acid bacteria. This was diluted 3-fold (6 mL of PBS was added to 3 mL of 0.01% by mass lactic acid bacteria growth composition) to obtain a 0.003% by mass lactic acid bacteria growth composition (9 mL). This was dispensed into a conical tube and autoclaved at 121 ° C. for 20 minutes.
−乳酸菌の前培養−
ストレプトコッカス・フェリカスの粉末を、食品衛生検査指針に準じて作製したリン酸緩衝生理食塩水にて溶解し、ＢＣＰ培地（メルク社製）にて培養を行い、理論値が３×１０６個／ｍＬとなるようにＰＢＳを用いて菌数を調製した。
-Pre-culture of lactic acid bacteria-
Streptococcus ferricus powder is dissolved in phosphate buffered saline prepared according to food hygiene inspection guidelines, cultured in BCP medium (Merck), and the theoretical value is 3 × 10 6 cells / mL. The number of bacteria was adjusted using PBS so that
−乳酸菌の本培養−
この乳酸菌の調製液（３×１０６個／ｍＬ）を、滅菌済の０．００３質量％乳酸菌増殖用組成物（９ｍＬ）を含むコニカルチューブに、３０μＬ接種した。これを３５℃にて０日間〜２日間、静置培養を行った。
-Main culture of lactic acid bacteria-
30 μL of this lactic acid bacteria preparation (3 × 10 6 cells / mL) was inoculated into a sterilized conical tube containing a 0.003% by mass composition for lactic acid bacteria growth (9 mL). This was statically cultured at 35 ° C. for 0 to 2 days.
−菌数の確認−
培養０日目（接種直後）、培養１日目（２４時間後）、及び培養２日目（４８時間後）の各試料を、綿棒を用いてＢＣＰ培地（メルク社製）に塗抹後、上下転倒し、３５℃、２４時間〜４８時間培養を行い、菌の増減を確認することにより、乳酸菌増殖試験を行った。結果を図１に示す。
-Confirmation of the number of bacteria-
After smearing each sample on day 0 of culture (immediately after inoculation), day 1 of culture (after 24 hours) and day 2 of culture (after 48 hours) onto BCP medium (Merck) using a cotton swab, A lactic acid bacteria growth test was performed by inverting, culturing at 35 ° C. for 24 to 48 hours, and confirming the increase or decrease of the bacteria. The results are shown in FIG.
（実施例２）
＜乳酸菌増殖試験（大麦若葉＋ＰＢＳ）＞
実施例１における［大麦若葉粉末１］を、［大麦若葉粉末２］に変更したこと以外は、実施例１と同様にして、実施例２の乳酸菌増殖試験を行った。結果を図１に示す。
(Example 2)
<Lactic acid bacteria growth test (barley young leaves + PBS)>
The lactic acid bacteria growth test of Example 2 was conducted in the same manner as in Example 1 except that [Barley young leaf powder 1] in Example 1 was changed to [Barley young leaf powder 2]. The results are shown in FIG.
（実施例３）
＜乳酸菌増殖試験（大麦若葉＋ＰＢＳ）＞
実施例１における［大麦若葉粉末１］を、［大麦若葉粉末３］に変更したこと以外は、実施例１と同様にして、実施例３の乳酸菌増殖試験を行った。結果を図１に示す。
(Example 3)
<Lactic acid bacteria growth test (barley young leaves + PBS)>
A lactic acid bacteria growth test of Example 3 was performed in the same manner as in Example 1 except that [Barley young leaf powder 1] in Example 1 was changed to [Barley young leaf powder 3]. The results are shown in FIG.
（比較例１）
＜乳酸菌増殖試験（オリゴ糖＋ＰＢＳ）＞
実施例１における乳酸菌増殖用組成物を、０．００３質量％のオリゴ糖を含むＰＢＳに変更したこと以外は、実施例１と同様にして、比較例１の乳酸菌増殖試験を行った。結果を図１に示す。なお、オリゴ糖は、乳酸菌が資化できる糖類であり、乳酸菌の増殖によく用いられる物質である。
(Comparative Example 1)
<Lactic acid bacteria growth test (oligosaccharide + PBS)>
A lactic acid bacteria growth test of Comparative Example 1 was conducted in the same manner as in Example 1 except that the composition for growing lactic acid bacteria in Example 1 was changed to PBS containing 0.003 mass% oligosaccharide. The results are shown in FIG. Oligosaccharide is a saccharide that can be assimilated by lactic acid bacteria, and is a substance often used for the growth of lactic acid bacteria.
（参考例１）
＜乳酸菌増殖試験（ＰＢＳのみ）＞
実施例１における乳酸菌増殖用組成物を、ＰＢＳのみに変更したこと以外は、実施例１と同様にして、参考例１の乳酸菌増殖試験を行った。結果を図１に示す。
(Reference Example 1)
<Lactic acid bacteria growth test (PBS only)>
The lactic acid bacteria growth test of Reference Example 1 was conducted in the same manner as in Example 1 except that the composition for growing lactic acid bacteria in Example 1 was changed to only PBS. The results are shown in FIG.
図１より、実施例１〜３の乳酸菌増殖用組成物（ＰＢＳに大麦若葉の粉末を添加した液体培地）を用いることにより、比較例１と比べ、培養条件が制限されることなく、また、大麦若葉粉末の製造時期に関わらず、安価かつ簡便に、乳酸菌を増殖させることができることがわかった。また、乳酸菌増殖用組成物を用いて２日間培養を行った乳酸菌を用いることにより、０日間培養や１日間培養と比較して、乳酸菌の増殖がより顕著となることがわかった。 From FIG. 1, by using the composition for growing lactic acid bacteria of Examples 1 to 3 (liquid medium in which barley young powder was added to PBS), the culture conditions were not limited as compared with Comparative Example 1, It was found that lactic acid bacteria can be propagated inexpensively and easily regardless of the production time of the barley young leaf powder. It was also found that the use of lactic acid bacteria that had been cultured for 2 days using the composition for growing lactic acid bacteria resulted in more prominent growth of lactic acid bacteria than 0-day culture or 1-day culture.
（試験例２：乳酸菌増殖試験２）
試験例２では、大麦若葉による乳酸菌の増殖を計数した。
(Test Example 2: Lactic acid bacteria growth test 2)
In Test Example 2, the growth of lactic acid bacteria by young barley leaves was counted.
（実施例４）
＜乳酸菌増殖試験（大麦若葉＋ＰＢＳ）＞
−乳酸菌増殖用組成物の調製方法−
コニカルチューブに下記［１］〜［２］のように調製した溶液を、オートクレーブ（１２１℃、２０分間）にかけて大麦若葉粉末を０．００３質量％含有する乳酸菌増殖用組成物とした。
［１］リン酸生理食塩水１０ｍＬの１質量％である［大麦若葉粉末４］０．１ｇを添加した。
［２］［１］を１００倍希釈して大麦若葉粉末の濃度を０．０１質量％に調製し、更に３倍希釈して、大麦若葉粉末の濃度が０．００３質量％となるように調製した。
Example 4
<Lactic acid bacteria growth test (barley young leaves + PBS)>
-Preparation method of lactic acid bacteria growth composition-
The solution prepared as described in [1] to [2] below in a conical tube was subjected to an autoclave (121 ° C., 20 minutes) to obtain a composition for growing lactic acid bacteria containing 0.003% by mass of barley young leaf powder.
[1] 0.1 g of [Barley young leaf powder 4] which is 1% by mass of 10 mL of phosphate physiological saline was added.
[2] [1] is diluted 100 times to prepare a barley young leaf powder concentration of 0.01% by mass, and further diluted 3 times to prepare a barley young leaf powder concentration of 0.003% by mass. did.
−乳酸菌の前培養−
グリセロールストックとして冷凍保管されているストレプトコッカス・フェカリスの保存菌株を、１０ｍＬのＭＲＳ培地（メルク社製）にて３５℃で一晩培養して、種菌培養液とした。この種菌培養液を乳酸菌数の理論値が１０５個／ｍＬとなるようにリン酸生理食塩水を用いて乳酸菌の調製液を調製した。
-Pre-culture of lactic acid bacteria-
A stock strain of Streptococcus faecalis that was frozen and stored as a glycerol stock was cultured overnight at 35 ° C. in 10 mL of MRS medium (manufactured by Merck & Co., Inc.) to obtain an inoculum culture solution. The inoculum culture theoretical value of the number of lactic acid bacteria to prepare a preparation of lactic acid bacteria using 10 5 cells / mL to become as phosphate saline.
−乳酸菌の本培養−
この乳酸菌の調製液（１０５個／ｍＬ）を０．００３質量％の乳酸菌増殖用組成物（１０ｍＬ）を含むコニカルチューブに、１００μＬ接種した。これを３５℃にて０日間〜２日間、静置培養した。
-Main culture of lactic acid bacteria-
100 μL of the lactic acid bacteria preparation solution (10 5 cells / mL) was inoculated into a conical tube containing 0.003% by mass of the lactic acid bacteria growth composition (10 mL). This was statically cultured at 35 ° C. for 0 to 2 days.
−菌数の計測−
培養０日目（接種直後）、培養１日目（２４時間後）に、濃度調整した培養液（１００μＬ）を綿棒によりＢＣＰ培地（メルク社製）に塗抹後、上下転倒して３５℃で４８時間培養し、菌の増減を確認した。結果を表１に示す。
-Counting the number of bacteria-
On the 0th day of culture (immediately after inoculation) and 1st day of culture (after 24 hours), the culture solution (100 μL) whose concentration was adjusted was smeared onto the BCP medium (Merck) with a cotton swab and then turned upside down at 35 ° C. for 48 The culture was continued for an hour, and the increase or decrease of the bacteria was confirmed. The results are shown in Table 1.
（比較例２）
＜乳酸菌増殖試験（オリゴ糖＋ＰＢＳ）＞
実施例４における０．００３質量％の乳酸菌増殖用組成物（１０ｍＬ）を、０．００３質量％オリゴ糖を含むＰＢＳ溶液（１０ｍＬ）に変更したこと以外は、実施例４と同様にして比較例２の乳酸菌増殖試験を行った。結果を表１に示す。なお、オリゴ糖は、乳酸菌が資化できる糖類であり、乳酸菌の増殖によく用いられる物質である。
(Comparative Example 2)
<Lactic acid bacteria growth test (oligosaccharide + PBS)>
Comparative Example in the same manner as in Example 4 except that the 0.003 mass% composition for growing lactic acid bacteria (10 mL) in Example 4 was changed to a PBS solution (10 mL) containing 0.003 mass% oligosaccharide. Two lactic acid bacteria growth tests were performed. The results are shown in Table 1. Oligosaccharide is a saccharide that can be assimilated by lactic acid bacteria, and is a substance often used for the growth of lactic acid bacteria.
（比較例３）
＜乳酸菌増殖試験（ＭＲＳ培地＋ＰＢＳ）＞
実施例４における０．００３質量％の乳酸菌増殖用組成物（１０ｍＬ）を、ＭＲＳ培地（１０ｍＬ，規定濃度５．２２％，メルク社製）に変更したこと以外は、実施例４と同様にして比較例３の乳酸菌増殖試験を行った。結果を表１に示す。なお、ＭＲＳ培地は、乳酸菌の良好な発育を支持する培地として使用される培地である。
(Comparative Example 3)
<Lactic acid bacteria growth test (MRS medium + PBS)>
Except that the 0.003% by mass composition for lactic acid bacteria growth (10 mL) in Example 4 was changed to MRS medium (10 mL, normal concentration 5.22%, manufactured by Merck & Co., Inc.), it was the same as Example 4. The lactic acid bacteria growth test of Comparative Example 3 was performed. The results are shown in Table 1. The MRS medium is a medium used as a medium that supports the good growth of lactic acid bacteria.
（参考例２）
＜乳酸菌増殖試験（ＰＢＳのみ）＞
実施例４における０．００３質量％の乳酸菌増殖用組成物（１０ｍＬ）を、リン酸生理食塩水（１０ｍＬ）に変更したこと以外は、実施例４と同様にして参考例２の乳酸菌増殖試験を行った。結果を表１に示す。
(Reference Example 2)
<Lactic acid bacteria growth test (PBS only)>
The lactic acid bacteria growth test of Reference Example 2 was conducted in the same manner as in Example 4 except that the 0.003% by mass composition for lactic acid bacteria growth (10 mL) in Example 4 was changed to phosphate physiological saline (10 mL). went. The results are shown in Table 1.
表１より、大麦若葉を用いて乳酸菌を培養すると、長時間安定して乳酸菌が増殖されることがわかった。 From Table 1, it was found that when lactic acid bacteria were cultured using young barley leaves, the lactic acid bacteria were proliferated stably for a long time.
（試験例３：乳酸菌増殖試験３）
試験例３では、大麦若葉の用量に伴う乳酸菌の増殖を計数した。
(Test Example 3: Lactic acid bacteria growth test 3)
In Test Example 3, the growth of lactic acid bacteria with the dose of barley young leaves was counted.
（実施例５）
＜乳酸菌増殖試験（大麦若葉＋ＰＢＳ）＞
実施例４における［大麦若葉粉末４］を０．００３質量％含有する乳酸菌増殖用組成物（１０ｍＬ）を、乳酸菌増殖用組成物における［大麦若葉粉末４］の含有量が０．０００１質量％である乳酸菌増殖用組成物（１０ｍＬ）に変更したこと以外は、実施例４と同様にして実施例５の乳酸菌増殖試験を行った。結果を表２に示す。
(Example 5)
<Lactic acid bacteria growth test (barley young leaves + PBS)>
In the composition for lactic acid bacteria growth (10 mL) containing 0.003 mass% of [Barley young leaf powder 4] in Example 4, the content of [Barley young leaf powder 4] in the lactic acid bacteria growth composition was 0.0001 mass%. A lactic acid bacteria growth test of Example 5 was performed in the same manner as in Example 4 except that the composition was changed to a certain lactic acid bacteria growth composition (10 mL). The results are shown in Table 2.
（実施例６）
＜乳酸菌増殖試験（大麦若葉＋ＰＢＳ）＞
実施例４における［大麦若葉粉末４］を０．００３質量％含有する乳酸菌増殖用組成物（１０ｍＬ）を、乳酸菌増殖用組成物における［大麦若葉粉末４］の含有量が０．００１質量％である乳酸菌増殖用組成物（１０ｍＬ）に変更したこと以外は、実施例４と同様にして実施例６の乳酸菌増殖試験を行った。結果を表２に示す。
(Example 6)
<Lactic acid bacteria growth test (barley young leaves + PBS)>
The composition for lactic acid bacteria growth (10 mL) containing 0.003% by mass of [Barley young leaf powder 4] in Example 4 was 0.001% by mass in the content of [Barley young leaf powder 4] in the lactic acid bacteria growth composition. The lactic acid bacteria growth test of Example 6 was performed in the same manner as in Example 4 except that the composition was changed to a certain composition for lactic acid bacteria growth (10 mL). The results are shown in Table 2.
（実施例７）
＜乳酸菌増殖試験（大麦若葉＋ＰＢＳ）＞
実施例４における［大麦若葉粉末４］を０．００３質量％含有する乳酸菌増殖用組成物（１０ｍＬ）を、乳酸菌増殖用組成物における［大麦若葉粉末４］の含有量が０．０１質量％である乳酸菌増殖用組成物（１０ｍＬ）に変更したこと以外は、実施例４と同様にして実施例７の乳酸菌増殖試験を行った。結果を表２に示す。
(Example 7)
<Lactic acid bacteria growth test (barley young leaves + PBS)>
In the composition for lactic acid bacteria growth (10 mL) containing 0.003 mass% of [Barley young leaf powder 4] in Example 4, the content of [Barley young leaf powder 4] in the lactic acid bacteria growth composition was 0.01 mass%. The lactic acid bacteria growth test of Example 7 was performed in the same manner as in Example 4 except that the composition was changed to a certain lactic acid bacteria growth composition (10 mL). The results are shown in Table 2.
（実施例８）
＜乳酸菌増殖試験（大麦若葉＋ＰＢＳ）＞
実施例４における［大麦若葉粉末４］を０．００３質量％含有する乳酸菌増殖用組成物（１０ｍＬ）を、乳酸菌増殖用組成物における［大麦若葉粉末４］の含有量が０．１質量％である乳酸菌増殖用組成物（１０ｍＬ）に変更したこと以外は、実施例４と同様にして実施例８の乳酸菌増殖試験を行った。結果を表２に示す。
(Example 8)
<Lactic acid bacteria growth test (barley young leaves + PBS)>
In the composition for lactic acid bacteria growth (10 mL) containing 0.003 mass% of [Barley young leaf powder 4] in Example 4, the content of [Barley young leaf powder 4] in the lactic acid bacteria growth composition was 0.1 mass%. A lactic acid bacteria growth test of Example 8 was conducted in the same manner as in Example 4 except that the composition was changed to a certain lactic acid bacteria growth composition (10 mL). The results are shown in Table 2.
（実施例９）
＜乳酸菌増殖試験（大麦若葉＋ＰＢＳ）＞
実施例４における［大麦若葉粉末４］を０．００３質量％含有する乳酸菌増殖用組成物（１０ｍＬ）を、乳酸菌増殖用組成物における［大麦若葉粉末４］の含有量が１．０質量％である乳酸菌増殖用組成物（１０ｍＬ）に変更したこと以外は、実施例４と同様にして実施例９の乳酸菌増殖試験を行った。結果を表２に示す。
Example 9
<Lactic acid bacteria growth test (barley young leaves + PBS)>
The composition for lactic acid bacteria growth (10 mL) containing 0.003% by mass of [Barley young leaf powder 4] in Example 4 was 1.0% by mass in the content of [Barley young leaf powder 4] in the lactic acid bacteria growth composition. The lactic acid bacteria growth test of Example 9 was conducted in the same manner as in Example 4 except that the composition was changed to a certain lactic acid bacteria growth composition (10 mL). The results are shown in Table 2.
乳酸菌の増殖は、大麦若葉の含有量が高い程、顕著となることがわかった。
また、大麦若葉及びＰＢＳを含有する乳酸菌増殖用組成物に、更にオリゴ糖及び水溶性食物繊維を含有させると、乳酸菌（ストレプトコッカス・フェカリス）の増殖がより顕著となることがわかった。
また、大麦若葉及びＰＢＳを含有する乳酸菌増殖用組成物に、更に公知の乳酸菌、オリゴ糖及び水溶性食物繊維を含有させると、乳酸菌（ストレプトコッカス・フェカリス）の増殖がより顕著となることがわかった。
It was found that the growth of lactic acid bacteria became more remarkable as the content of young barley leaves increased.
It was also found that when the composition for growing lactic acid bacteria containing barley young leaves and PBS was further added with oligosaccharide and water-soluble dietary fiber, the growth of lactic acid bacteria (Streptococcus faecalis) became more remarkable.
In addition, it was found that the growth of lactic acid bacteria (Streptococcus faecalis) becomes more prominent when a known lactic acid bacterium, oligosaccharide and water-soluble dietary fiber are further added to the composition for growing lactic acid bacteria containing barley young leaves and PBS. .
本発明の乳酸菌増殖用組成物を用いることにより、安価かつ簡便に、乳酸菌を増殖させることができるので、例えば、乳酸菌を含有する素材、食品、食品素材、食品組成物等の添加物として好適に利用することができる。また、同様に、本発明の乳酸菌増殖用組成物を含有する乳酸菌用培地を用いることにより、安価かつ簡便に、乳酸菌を増殖させることができるため、乳酸菌の培養に好適に用いることができ、安全性にも優れるため、例えば、乳酸菌を含有する素材、食品、食品素材、食品組成物等の添加物として好適に利用することができる。 By using the composition for growing lactic acid bacteria of the present invention, the lactic acid bacteria can be propagated inexpensively and easily. For example, it is suitably used as an additive for materials containing lactic acid bacteria, foods, food materials, food compositions, etc. Can be used. Similarly, by using the medium for lactic acid bacteria containing the composition for growing lactic acid bacteria of the present invention, the lactic acid bacteria can be propagated inexpensively and easily, so that it can be suitably used for culturing lactic acid bacteria and is safe. For example, it can be suitably used as an additive for materials containing lactic acid bacteria, foods, food materials, food compositions, and the like.
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| JP5598943B1 (en) * | 2013-05-29 | 2014-10-01 | 株式会社東洋新薬 | Composition for growing lactic acid bacteria |
| JP2015126723A (en) * | 2013-05-29 | 2015-07-09 | 株式会社東洋新薬 | Edible composition, method for improving taste and color of edible composition, composition for growth of lactic acid bacterium, culture method of foliage of barley, and foliage of barley cultured by using andosols or aso-grown soil |
| JP5692549B2 (en) * | 2013-09-18 | 2015-04-01 | 株式会社東洋新薬 | Composition for intestinal cell activation |
| JP5887470B1 (en) * | 2015-02-19 | 2016-03-16 | 株式会社東洋新薬 | Barley stems and / or leaves from Aso |
| JP5906510B1 (en) * | 2015-06-12 | 2016-04-20 | 株式会社東洋新薬 | Green leaf powder for food and drink and method for improving drinking of food and drink |
| JP2021035346A (en) * | 2019-08-30 | 2021-03-04 | 国立大学法人神戸大学 | Composition for intestinal regulation, method of culturing probiotic bacteria, and food and drink, quasi drug, or pharmaceutical |
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| JPN6013029353; Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ Ｖｏｌ．１７４, 1948, ｐ．４０５-４０９ * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018000157A (en) * | 2016-07-07 | 2018-01-11 | 株式会社東洋新薬 | Ferulic acid-containing beverage |
| JP7122727B2 (en) | 2016-07-07 | 2022-08-22 | 株式会社東洋新薬 | Beverage containing ferulic acid |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2013031426A (en) | 2013-02-14 |
| JP5544665B2 (en) | 2014-07-09 |
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