JP2014101313A - Skin topical agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a skin topical agent having excellent resveratrol percutaneous absorption action, allowing sufficient achievement of the effect of resveratrol desired at the time of application.SOLUTION: The skin topical agent of the invention comprises fine powder of resveratrol containing plant extract. Further, the skin emulsifier of the invention further comprises emulsifier, and the content of the emulsifier is preferably 1.0 mass% - 5.0 mass%. Further, in the skin topical agent of the invention, the number average particle diameter(D) of fine powder of resveratrol containing plant extract is preferably 0.5 μm - 5.0 μm.

Description

  The present invention relates to an external preparation for skin.

  Conventionally, polyphenols have attracted attention as useful substances contained in natural products, particularly plants, and their research and development have been continued, and are known to have various types.

  As the types of the polyphenols, for example, flavonoids such as marignans, curcumins, coumarins, catechins, tannins, anthocyanins, proanthocyanidins, rutin, quercetin, resveratrol and the like are known, among these, Resveratrol has attracted attention.

  However, polyphenols such as resveratrol have a high molecular weight and are difficult to dissolve in water and organic solvents. Therefore, their absorbability to skin is low and the effects of resveratrol expected at the time of application are low. There is a problem that it cannot be fully demonstrated.

  Therefore, the present situation is that there is a strong demand for rapid development of a topical skin preparation that is excellent in the transdermal absorption action of resveratrol and that can sufficiently exhibit the effects of resveratrol expected at the time of application.

JP 2008-88123 A

  An object of the present invention is to solve the above-described problems and achieve the following objects. That is, an object of the present invention is to provide a skin external preparation that is excellent in the transdermal absorption action of resveratrol and can sufficiently exhibit the effect of resveratrol expected at the time of application.

  As a result of intensive studies to achieve the above object, the inventors of the present invention, when using a resveratrol-containing plant extract fine powder as an external preparation for skin, excels in the transdermal absorption of resveratrol, It has been found that it becomes a skin external preparation capable of sufficiently exhibiting the expected effect of resveratrol, and the present invention has been completed.

The present invention is based on the above findings by the present inventors, and means for solving the above problems are as follows. That is,
<1> A skin external preparation characterized by containing a fine powder of a resveratrol-containing plant extract.
<2> The skin external preparation according to <1>, further including an emulsifier.
<3> The skin external preparation according to <2>, wherein the content of the emulsifier is 1.0% by mass to 5.0% by mass.
Is <4> number average particle size of fine powder _{(D 90),} a skin external preparation according to any one of <3>, wherein a 0.5μm~5.0μm <1>.
<5> From the above <1> to <1>, wherein the resveratrol-containing plant extract is at least one of a lingonberry extract, a cowberry extract, a grape extract, a cowberry extract, an Indian mushroom tree extract, and a meringo extract 4> is an external preparation for skin.
<6> The skin external preparation according to any one of <2> to <5>, wherein the emulsifier is an O / W type emulsifier.
<7> The skin external preparation according to any one of <2> to <6>, wherein the emulsifier contains at least alcohols, fatty acid esters, hydrocarbons, and lecithins.
<8> The skin external preparation according to <7>, wherein the alcohol is at least one of glycerin, cetanol, and pentylene glycol.
<9> The skin external application according to any one of <7> to <8>, wherein the fatty acid ester is at least one of polyglyceryl-10 laurate, tri (capsule acid / capric acid) glyceryl, and macadamia nut fatty acid phytosteryl. It is an agent.
<10> The skin external preparation according to any one of <7> to <9>, wherein the hydrocarbon is squalane.
<11> The skin external preparation according to any one of <7> to <10>, wherein the lecithin is hydrogenated lecithin.

  According to the present invention, the above-described problems can be solved and the above-mentioned object can be achieved, the resveratrol is excellent in transdermal absorption, and the effects of resveratrol expected at the time of application are sufficiently exhibited. An external preparation for skin can be provided.

FIG. 1 is a graph showing the results of the transdermal absorbability test of Test Example 1. FIG. 2 is a graph showing the results of the transdermal absorbability test of Test Example 2. FIG. 3 is a graph showing the results of the transdermal absorbability test of Test Example 3.

(Skin external preparation)
The external preparation for skin of the present invention comprises a fine powder of resveratrol-containing plant extract, preferably contains an emulsifier, and further contains other components as necessary.

<Fine powder of resveratrol-containing plant extract>
The resveratrol is a kind of stilbenoid (stilbene derivative) polyphenol, the system name is 3,5,4′-trihydroxy-trans-stilbene, and is a compound represented by the following structural formula (1). .

  The fine powder of the resveratrol-containing plant extract is not particularly limited as long as it is a fine powder of the plant extract containing resveratrol, and can be appropriately selected according to the purpose. In addition, as the fine powder of the resveratrol-containing plant extract, a fine powder of the resveratrol compound (compound represented by the structural formula 1) may be used, and the composition contains the resveratrol. A fine powder of a product (resveratrol-containing composition) may be used, or a fine powder combining the resveratrol compound and the resveratrol-containing composition may be used.

  The resveratrol-containing plant extract can be easily obtained by utilizing a method generally used for plant extraction, but a commercially available product may be used.

  For the resveratrol-containing plant extract, a resveratrol-containing plant extract, a diluted or concentrated solution of the extract, a dried product of the extract, or a crudely purified product or a purified product thereof is used. Also good. The resveratrol-containing plant extract is then pulverized by a preparation method described later.

The resveratrol-containing plant is not particularly limited as long as it is a plant containing the resveratrol, and can be appropriately selected according to the purpose. For example, cowberry ( Vaccinium vitis-idaea L.), grape ( Vitis vinifera L.), Indian mushroom tree ( Pterocarpus marsupium ), Melinjo ( Gnetum gnemon Linn) and the like. These may be used individually by 1 type and may use 2 or more types together. Among these, cowberry or grape is preferable.

The subspecies of cowberry which is the resveratrol-containing plant is not particularly limited and can be appropriately selected according to the purpose. For example, Lingonberry ( Vaccinium vitis-idaea var. Minus Rodd.), Cowberry ( Vaccinium ) vitis - idaea var. vitis - idaea L.) and the like. These may be used individually by 1 type and may use 2 or more types together. Among these, Lingonberry ( Vaccinium vitis-idaea var. Minus Rodd.) Is preferable in that the effect of resveratrol can be sufficiently exhibited.

  The extraction site of the resveratrol-containing plant extract is not particularly limited and may be appropriately selected depending on the intended purpose. However, a site containing a large amount of resveratrol is preferred, and the bilberry and the lingonberry When extracting, a fruit and a skin part are more preferable at the point which contains the said resveratrol much, and when extracting from the said grape, a stem part is more preferable at the point which contains the said resveratrol much.

  The method for preparing the extraction site of the resveratrol-containing plant extract is not particularly limited and can be appropriately selected depending on the purpose.In terms of improving the extraction efficiency and production stability of the resveratrol, A method of preparing the extraction part by subdividing or squeezing is preferable. Specifically, a method of subtracting or squeezing the extraction part by a machine (mixer or the like), and subtracting or squeezing the extraction part manually. And a method of preparing them.

  The method for extracting the resveratrol-containing plant is not particularly limited, and a known method can be used according to the purpose.For example, after adding an extraction solvent to the extracted site of the prepared plant and stirring, Examples include a method of performing extraction by solid-liquid separation with a centrifuge (such as a decanter continuous horizontal centrifuge, an automatic basket centrifuge, etc.). These extractions may be performed using one type of centrifuge, or in combination with two or more types of centrifuges in order to obtain resveratrol-containing extracts having various compositions. Good.

  There is no restriction | limiting in particular as an extraction solvent of the said resveratrol containing plant extract, According to the objective, it can select suitably, For example, water, an organic solvent, the mixed solvent of water and an organic solvent, etc. are mentioned. These may be used individually by 1 type and may use 2 or more types together.

  There is no restriction | limiting in particular as water which is an extraction solvent of the said resveratrol containing plant extract, According to the objective, it can select suitably, For example, cold water, such as tap water, distilled water, mineral water, alkali ion water , Hot water, hot water and the like. Among these, hot water is preferable. There is no restriction | limiting in particular as temperature of the said hot water (water temperature is 50 degreeC or more), Although it can select suitably according to the objective, 50 to 120 degreeC is a point excellent in the extraction efficiency of a bioactive substance. Is preferable, and 70 to 100 ° C is more preferable.

  There is no restriction | limiting in particular as an organic solvent which is an extraction solvent of the said resveratrol containing plant extract, According to the objective, it can select suitably, For example, it is accept | permitted in manufacture of foodstuffs or chemical | medical agents, such as alcohol and edible oil and fat. Organic solvents and the like. Among these, ethanol is preferable.

  There is no restriction | limiting in particular as a mixed solvent of the water and organic solvent which are the extraction solvents of the said resveratrol containing plant extract, According to the objective, it can select suitably, For example, hydrous alcohol (alcohol aqueous solution etc.) etc. Is mentioned. Among these, hydrous ethanol is preferable. There is no restriction | limiting in particular as alcohol concentration of the said hydrous alcohol, According to the objective, it can select suitably.

  There is no restriction | limiting in particular as a form of the said resveratrol containing plant extract, According to the objective, it can select suitably, For example, a solution form, suspension form, semi-solid form (gel form, paste form, etc.), Solid (powder, granule, etc.) and the like can be mentioned. Among these, the powder form is preferred in that the resveratrol is excellent in transdermal absorbability and the fine powder described later can be easily prepared.

  The resveratrol-containing plant extract may be purified for the purpose of increasing the content of resveratrol. The purification method is not particularly limited and may be appropriately selected depending on the intended purpose.For example, a method for purification using a solvent, a method for purification without using a solvent, for example, ultrafiltration, Examples thereof include a column method and a batch method using an adsorptive carrier (a synthetic adsorbent such as Diaion (registered trademark) HP-20, Sephadex (registered trademark) LH20, or a natural adsorbent such as chitin).

<< Preparation Method of Fine Powder of Resveratrol-Containing Plant Extract >>
The method for preparing the fine powder of the resveratrol-containing plant extract is not particularly limited and can be appropriately selected depending on the purpose. For example, the granule obtained by freeze-drying the resveratrol-containing plant extract Examples thereof include a wet method in which a body is dissolved in a solvent and refined; a dry method in which the powdered product obtained by freeze-drying the resveratrol-containing plant extract is further refined. Among these, the dry method is preferable in that the extraction efficiency of the resveratrol is excellent and the manufacturing process such as drying the resveratrol-containing plant extract can be omitted.

  There is no restriction | limiting in particular as time which freeze-drying performed when preparing the fine powder of the said resveratrol containing plant extract, According to the objective, it can select suitably.

  There is no restriction | limiting in particular as a grinding | pulverization apparatus used when the said resveratrol containing plant extract is refined | miniaturized, According to the objective, a commercially available thing can be selected suitably, for example, one or more small diameters in a high pressure atmosphere High-pressure homogenizer that pulverizes an object by moving fluid at high speed in a nozzle having a hole and a specific flow path; Ultrasonic pulverizer that pulverizes an object using ultrasonic waves; Airflow type pulverizer that generates eddy currents and atomizes the object by the action of impact, shear, compression, grinding, high frequency vibration, etc .; High-speed rotary impact pulverizer that pulverizes the object by high-speed stirring or impact; Examples thereof include a ball mill or a bead mill to be used.

  The refining conditions of the resveratrol-containing plant extract are not particularly limited as long as the resveratrol-containing plant extract can be refined, and the crushing apparatus, the type of nozzle of the crushing apparatus, It can select suitably according to the particle size etc. of the said extract before a grinding | pulverization process.

The number of times of the finely pulverizing treatment of the resveratrol-containing plant extract is not particularly limited and can be appropriately selected according to the purpose, but the number average particle diameter of the resveratrol-containing plant extract fine powder One time may be sufficient so that ( _D90 ) may be in a preferable range, and it may be twice or more as needed.

-Number average particle diameter ( _D90 ) of fine powder of resveratrol-containing plant extract-
The number average particle size (D ₉₀ : particle size when the integrated value is 90%) of the fine powder of the resveratrol-containing plant extract is not particularly limited and may be appropriately selected depending on the purpose. 0.5 μm to 5.0 μm is preferable. The number average particle diameter (D ₉₀ ) can be measured using a laser diffraction / scattering particle size distribution analyzer.

<< Content of fine powder of resveratrol-containing plant extract >>
There is no restriction | limiting in particular as content in the said skin external preparation of the fine powder of the said resveratrol containing plant extract, Although it can select suitably according to the objective of the skin external preparation to be used, it is 0.00001 mass%. The above is preferable, 0.1 mass% or more is more preferable, and 0.5 mass% or more is still more preferable.

<< Resveratrol content >>
There is no restriction | limiting in particular as content of the said resveratrol in the fine powder of the said resveratrol containing plant extract, According to the objective, it can select suitably.

<Emulsifier>
The emulsifier is an additive used for emulsifying (emulsifying) the external preparation for skin. By using the emulsifier, an oil-in-water type (O / W type) skin external preparation further excellent in transdermal absorption of resveratrol is obtained.

  The emulsifier is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include an oil-in-water type (O / W type) emulsifier and a water-in-oil type (W / O type) emulsifier. Can be mentioned. Among these, an oil-in-water type (O / W type) emulsifier is preferable because it is excellent in the transdermal absorption action of resveratrol.

  There is no restriction | limiting in particular as content in the said skin external preparation of the said emulsifier, Although it can select suitably according to the objective, 1.0 mass%-5 in the point which is excellent in the percutaneous absorption action of a resveratrol. 0.0 mass% is preferable, and 2.5 mass% to 3.5 mass% is more preferable.

  The emulsifier is not particularly limited as long as it is an emulsifier capable of emulsifying the external preparation for skin, and can be appropriately selected according to the purpose, but in terms of excellent transdermal absorption of resveratrol, It is preferable to contain at least alcohols, fatty acid esters, hydrocarbons, and lecithins, and may further contain water.

<< Alcohols >>
The alcohols are not particularly limited and may be appropriately selected depending on the purpose.For example, cetanol, pentylene glycol, glycerin, isopropyl alcohol, ethylene glycol, capryl alcohol, lauryl alcohol, myristyl alcohol, stearyl alcohol, Examples include oleyl alcohol and linoleyl alcohol. These may be used individually by 1 type and may use 2 or more types together. Among these, cetanol, pentylene glycol, and glycerin are preferable in that the transdermal absorption action of resveratrol is excellent.

  There is no restriction | limiting in particular as content in the said emulsifier of the said alcohol, According to the objective, it can select suitably.

<< Fatty acid esters >>
The fatty acid esters are not particularly limited and may be appropriately selected depending on the purpose. Examples thereof include polyglyceryl-10 laurate, tri (capsule acid / capric acid) glyceryl, macadamia nut fatty acid phytosteryl, glycerin fatty acid ester, poly Examples include glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, and propylene glycol fatty acid ester. These may be used individually by 1 type and may use 2 or more types together. Of these, polyglyceryl-10 laurate, tri (capsulic acid / capric acid) glyceryl, and macadamia nut fatty acid phytosteryl are preferred because of the excellent transdermal absorption of resveratrol.

  There is no restriction | limiting in particular as content in the said emulsifier of the said fatty acid esters, According to the objective, it can select suitably.

<< Hydrocarbons >>
The hydrocarbons are not particularly limited and may be appropriately selected depending on the intended purpose.For example, squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, Examples include petroleum jelly. These may be used individually by 1 type and may use 2 or more types together. Among these, squalane is preferable because it is excellent in the transdermal absorption action of resveratrol.

  There is no restriction | limiting in particular as content in the said emulsifier of the said hydrocarbons, According to the objective, it can select suitably.

<< Lecithins >>
There is no restriction | limiting in particular as said lecithin, According to the objective, it can select suitably, For example, hydrogenated lecithin, fractionated lecithin, an enzyme-degraded lecithin, etc. are mentioned. These may be used individually by 1 type and may use 2 or more types together. Among these, hydrogenated lecithin is preferable because it is excellent in the transdermal absorption action of resveratrol.

  There is no restriction | limiting in particular as content in the said emulsifier of the said lecithin, According to the objective, it can select suitably.

<< Water >>
The water is not particularly limited and may be appropriately selected according to the purpose.For example, pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, purified water, hot water, Examples include ion-exchanged water, physiological saline, phosphate buffer, and phosphate buffered saline. These may be used individually by 1 type and may use 2 or more types together.

  There is no restriction | limiting in particular as content in the said emulsifier, According to the objective, it can select suitably.

<Other ingredients>
The other components are not particularly limited as long as the effects of the present invention are not impaired, and can be appropriately selected according to the purpose. For example, water, fats and oils, waxes, polyhydric alcohols (glycerin, 1 , 3-butylene glycol, etc.), metal soaps, pH adjusters, preservatives, fragrances, powders, UV absorbers, thickeners, dyes, antioxidants, chelating agents, emulsion stabilizers, and the like. These may be used individually by 1 type and may use 2 or more types together.

<Method for producing external preparation for skin>
The method for producing the external preparation for skin is not particularly limited and may be appropriately selected depending on the intended purpose. The step of preparing a fine powder of the resveratrol-containing plant extract, and the base of the external preparation for skin It is preferable to include a step of blending the fine powder with respect to the material, and the emulsifier and the other components may be blended with the base material as necessary.

<How to use external preparation for skin>
The animal species to be used for the external preparation for skin is not particularly limited and may be appropriately selected depending on the intended purpose. However, it can be suitably applied to humans and has the respective effects. As long as it is applied to animals other than humans, such as mice, rats, hamsters, birds, dogs, cats, sheep, goats, cows, pigs, monkeys, and the like.
The use site of the external preparation for skin is not particularly limited as long as it is a site having skin, and can be appropriately selected according to the purpose. Examples thereof include hands, feet, face, back, scalp and the like.
The method for using the external preparation for skin is not particularly limited as long as it can be used for allowing the external preparation for skin to penetrate into the skin, and can be appropriately selected according to the purpose.
The amount of the external preparation for skin used is not particularly limited and can be appropriately selected in consideration of various factors such as the age, weight and constitution of the individual to be applied. The external preparation for skin is the resveratrol. Because of its excellent percutaneous absorbability, it may be smaller than the conventional coating amount.

<Application>
Since the external preparation for skin of the present invention is excellent in the transdermal absorbability of resveratrol and can sufficiently exhibit the effects of resveratrol expected at the time of application, an ointment, cream, and peeling containing resveratrol Cosmetics, emulsions, lotions, serums, packs, lip balms, sprays, liquid foundations, skin cosmetics such as lipsticks, hair styling, cleaning agents, cleansing agents, quasi-drugs such as bath preparations, pharmaceuticals, whitening agents, anti It can be suitably used for oxidizing agents, anti-aging agents, anti-obesity agents and the like.

  Examples of the present invention will be described below, but the present invention is not limited to these examples.

[Test Example 1: Transdermal absorption test of external preparation for skin]
The percutaneous absorption action test of each manufactured skin external preparation (no emulsifier added) was conducted.

<Manufacture of external preparation for skin>
Each skin external preparation prepared the aqueous solution by mixing or melt | dissolving resveratrol and water so that it might become a composition shown in Table 1, and manufactured the skin external preparation.
Here, the resveratrol used in Example 1 is a resveratrol-containing plant extract (Lingonberry extract, manufactured by BGG Japan Co., Ltd.) finely pulverized with a homogenizer (resveratrol-containing plant extract). Called fine powder). The number average particle size (D ₉₀ ) of the fine powder of the resveratrol-containing plant extract was 1.66 μm.
As the resveratrol used in Comparative Example 1, the resveratrol-containing plant extract used in Example 1 that was not finely ground (referred to as resveratrol powder) was used. The number average particle size (D ₉₀ ) of the resveratrol powder was 8.95 μm.
The number average particle size (D ₉₀ ) was measured using a laser diffraction / scattering particle size distribution analyzer.

<Test method of Test Example 1>
In the percutaneous absorption test, 50 μL of each prepared topical skin preparation was dropped on the upper layer of a three-dimensional skin model (Lab Cyte EPIMODEL 24, manufactured by Japan Tissue Engineering Co., Ltd., skin model diameter 6.4 mm), and 3 hours passed. The amount of polyphenol in phosphate buffered saline located below the later three-dimensional skin model was measured by the Folin-Denis method.
The amount of polyphenol by the Folin-Denis method was measured as follows. First, 25 μL of a test substance and 25 μL of phosphate buffered saline were added, 50 μL of 10 mass% sodium carbonate was added, and the mixture was left for 3 minutes. Next, 50 μL of Folin reagent was added and color was allowed to develop for 1 hour at room temperature. Then, after the colored substance to be tested was centrifuged (13,000 rpm, 5 minutes), 100 μL of the supernatant was transferred to a 96-well microplate, and the absorbance (wavelength 730 nm) was measured. From the obtained absorbance value, the amount of polyphenol (μg) contained in 1 mL of the test substance was converted based on a calibration curve.
The calibration curve used was each lingonberry extract (solution) prepared to 11.1 μg / mL, 3.70 μg / mL, 1.24 μg / mL, and 0.412 μg / mL. The results are shown in Table 1 and FIG.

<Results of Test Example 1>
From the above, it was suggested that percutaneous absorption of resveratrol is enhanced by using an external preparation for skin containing a fine powder of resveratrol-containing plant extract.

[Test Example 2: Transdermal absorption test of external preparation for skin]
The percutaneous absorption test of each manufactured external preparation for skin (with emulsifier added) was conducted.

<Manufacture of emulsifier>
The emulsifier (O / W type) is prepared by mixing or dissolving the components shown in Table 2-1 so as to have the composition shown in Table 2-1, and then using a wet atomizer (ultimizer system). It was manufactured by dispersing each component.

<Manufacture of external preparation for skin>
Each external preparation for skin was prepared by mixing or dissolving resveratrol, polyhydric alcohols (glycerin and 1,3-butylene glycol), and water so as to have the composition shown in Table 2-2. And the emulsifier manufactured above was added with respect to the said aqueous solution so that it might become a composition shown in Table 2-2, and it emulsified using the homogenizer, and manufactured the skin external preparation (emulsion skin external preparation). .
Here, the resveratrol used in Example 2 is the same as the resveratrol-containing plant extract fine powder (number average particle diameter (D ₉₀ ): 1.66 μm) used in Example 1. did. The resveratrol used in Comparative Example 2 was the same as the resveratrol powder used in Comparative Example 1 (number average particle diameter (D ₉₀ ): 8.95 μm).

<Test Method of Test Example 2>
In the percutaneous absorption test, 20 μL of each prepared skin external preparation was dropped on the upper layer of a three-dimensional skin model (Lab Cyte EPIMODEL 24, manufactured by Japan Tissue Engineering Co., Ltd., skin model diameter 6.4 mm) and passed for 3 hours. The amount of polyphenol in phosphate buffered saline located below the later three-dimensional skin model was measured by the Folin-Denis method.
The amount of polyphenol by the Folin-Denis method was measured as follows. First, the test substance was dried in a centrifugal evaporator, dissolved in 100 μL of purified water, 50 μL was measured into a 1.5 mL microtube, 50 μL of 10 mass% sodium carbonate was added, and the mixture was allowed to stand for 3 minutes. Next, 50 μL of Folin reagent was added and color was allowed to develop for 1 hour at room temperature. Then, after the colored substance to be tested was centrifuged (13,000 rpm, 5 minutes), 100 μL of the supernatant was transferred to a 96-well microplate, and the absorbance (wavelength 730 nm) was measured. From the obtained absorbance value, the amount of polyphenol (μg) contained in 1 mL of the test substance was converted based on a calibration curve.
The calibration curve used was each lingonberry extract (solution) prepared to 11.1 μg / mL, 3.70 μg / mL, 1.24 μg / mL, and 0.412 μg / mL. The results are shown in Table 2-2 and FIG.

<Results of Test Example 2>
From the above, the skin external preparation using the resveratrol-containing plant extract fine powder (with fine pulverization treatment) and the emulsifier together is the skin external preparation using the resveratrol powder (without fine pulverization treatment) and the emulsifier together. It was suggested that the transdermal absorption of resveratrol was significantly increased.

[Test Example 3: Transdermal absorption test of external preparation for skin]
The percutaneous absorption test of each manufactured external preparation for skin (with emulsifier added) was conducted.

<Manufacture of emulsifier>
The same emulsifier as that used in Test Example 2 was used.

<Manufacture of external preparation for skin>
Each external preparation for skin is prepared by mixing or dissolving a fine powder of resveratrol-containing plant extract, a polyhydric alcohol (glycerin and 1,3-butylene glycol), and water so as to have the composition shown in Table 3. An aqueous solution was prepared. And the emulsifier manufactured above was added with respect to the said aqueous solution so that it might become a composition shown in Table 3, and it emulsified using the homogenizer, and manufactured the skin external preparation (emulsion skin external preparation). Here, the fine powder of the resveratrol-containing plant extract is the same as the fine powder of the resveratrol-containing plant extract used in Example 1 (number average particle diameter (D ₉₀ ): 1.66 μm). It was used.

<Test method of Test Example 3>
The transdermal absorption test was performed in the same manner as in Test Example 2.
However, the amount of each external preparation for skin dripped onto the skin model was 5 μL.
The results are shown in Table 3 and FIG.

<Results of Test Example 3>
From the above, it was suggested that percutaneous absorption of resveratrol is remarkably increased by using a skin external preparation that uses a combination of a resveratrol-containing plant extract fine powder and an emulsifier.
Further, as can be seen from these results, when a skin external preparation having a content of the emulsifier in the external preparation for skin of 1.0% by mass to 5.0% by mass is used, the action of resveratrol percutaneously absorbs. It has been found that a particularly remarkable effect is exhibited.

  In addition, as resveratrol-containing plant extract, bilberry extract, grape extract, cowberry extract, Indian mushroom tree extract, and melinjo extract can be used in the same manner as in Test Examples 1 to 3, It was assumed that the result was excellent in transdermal absorption.

  Since the external preparation for skin of the present invention is excellent in the transdermal absorption action of resveratrol and can sufficiently exhibit the effect of resveratrol expected at the time of application, an ointment, cream, and peeling containing resveratrol Suitable for use in quasi-drugs such as skin preparations such as preparations, emulsions, lotions, cosmetics, packs, lip balms, sprays, liquid foundations, lipsticks, hair styling agents, cleaning agents, cleansing agents, bathing agents, etc. can do.

Claims (4)

  An external preparation for skin, comprising a fine powder of resveratrol-containing plant extract.   The skin external preparation according to claim 1, further comprising an emulsifier.   The skin external preparation according to claim 2, wherein the content of the emulsifier is 1.0% by mass to 5.0% by mass. Number average particle size of fine powder _{(D 90)} is the skin external preparation according to any of claims 1 to 3 is 0.5Myuemu～5.0Myuemu.