JP2014060983A - Device for detecting nucleic acid - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a device for detecting nucleic acid with improved reliability.SOLUTION: A device for detecting nucleic acid is equipped with a first sensor to which probes are immobilized, and which emits a signal when a nucleic acid as a detection target binds to the probe, second and third sensors each of which is provided independently of the first sensor, to which probes identical to ones immobilized to the first sensor are immobilized, and which emit a signal when a nucleic acid as a detection target binds to the probe, and a determination part to obtain each output value of the signals from the sensors. The first sensor is linked to the first detection electrode, the second sensor is linked to the second detection electrode, and the third sensor is linked to the third detection electrode. The determination part determines normality of the output value by comparing the output value taken from the first detection electrode, the output value taken from the second detection electrode, and the output value taken from the third detection electrode.

Description

  The present invention relates to a nucleic acid detection device used for genetic testing and the like.

  There is an automatic gene detection device for performing target detection by performing fully automatic detection and pretreatment steps of nucleic acids (see, for example, Patent Document 1).

Japanese Patent No. 2573443

  In industrial applications, genetic testing using a DNA chip requires performance such as miniaturization, high speed, automation, low cost, high reproducibility, and high sensitivity. The current detection method that detects the electrical signal from the labeling substance using an electrochemically active substance as the labeling substance not only eliminates the need for an optical system but also facilitates the labeling process. It is suitable. In such a genetic test, there is a need for high reliability.

  In the nucleic acid detection device of the embodiment, a first sensor that emits a signal when a probe is fixed and a nucleic acid to be detected is bound to the probe and the first sensor are provided independently, and the first sensor A second sensor and a third sensor that emit a signal when the same probe as the probe that is immobilized on the sensor is immobilized and a nucleic acid to be detected is bound to the probe; the first sensor; the second sensor; A first detection electrode, a second detection electrode, and a third detection electrode that obtain a signal from the third sensor; and connected to the first detection electrode, the second detection electrode, and the third detection electrode; 1 sensor, the second sensor, and a determination unit that obtains output values of signals from the third sensor, respectively, and the first sensor is connected to the first detection electrode, The second sensor is connected to the second detection electrode, the third sensor is connected to the third detection electrode, and the determination unit includes an output value extracted from the first detection electrode, and the second detection electrode. The output value taken out from the detection electrode and the output value taken out from the third detection electrode are compared to determine the normality of the output value.

The top view which shows the device for nucleic acid detection which concerns on 1st Embodiment. FIG. 2 is a plan view schematically showing an enlarged view of a reaction channel and a sensor periphery of the nucleic acid detection device shown in FIG. 1. The top view which expanded the reaction flow path and sensor circumference | surroundings of the nucleic acid detection device which concerns on 2nd Embodiment, and was shown typically. The top view which expanded the reaction flow path and sensor circumference | surroundings of the nucleic acid detection device which concerns on 3rd Embodiment, and was shown typically.

  Hereinafter, a first embodiment of a nucleic acid detection device will be described with reference to FIGS. 1 and 2. This nucleic acid detection device 11 contains all the reagents necessary for nucleic acid detection, and is mounted on a separately provided inspection apparatus 12 so that the process from nucleic acid amplification to detection is fully automated within one package. It can be carried out. The nucleic acid detection device 11 and the inspection apparatus 12 constitute an inspection system 10.

  FIG. 1 is a plan view (top view) of the nucleic acid detection device 11. In the present embodiment, the hard member 13 is, for example, transparent. In FIG. 1, the structure of each part (inside the nucleic acid detection device 11) that is transmitted through the hard member 13 and provided in the soft member 14 on the lower side. Structure) is shown. The hard member 13 may not be a transparent material.

  As shown in FIG. 1, the nucleic acid detection device 11 includes an upper hard member 13 and a lower soft member 14 having a joint surface 14 </ b> A that is abutted against the hard member 13. A reaction vessel is formed. The hard member 13 is made of a plastic material such as polycarbonate. Moreover, the soft member 14 is comprised with the elastic body which has rubber-like elasticity, such as a synthetic rubber, for example.

  The soft member 14 is provided with a reagent inflow syringe 15 for supplying and storing a sample and a test reagent, and a fluid discharge syringe 16 for discharging the reacted fluid. The reagent inflow syringe 15 and the fluid discharge syringe 16 are provided so as to be recessed from the joint surface 14A. As shown in FIG. 1, the reagent inflow syringe 15 is supplied with a sample (DNA sample) from the outside immediately before the start of the test, and a first syringe 15A for temporary storage, and a second syringe 15B for storing a cleaning liquid. A third syringe 15C in which an insertion agent for detection for electrically detecting amplified DNA is stored is included. In the present embodiment, the reagent inflow syringe 15 side is referred to as upstream, and the fluid discharge syringe 16 side is referred to as downstream.

  The soft member 14 further includes a reaction channel 21 (reaction unit) serving as a reaction field, a first channel 22 that connects the reagent inflow syringe 15 and the reaction channel 21, a reaction channel 21 and a fluid discharge syringe. 16, a first check valve 24 provided in the first flow path 22, and a second check valve 25 provided in the second flow path 23 are provided. The reaction channel 21, the first channel 22, and the second channel 23 are provided so as to be recessed from the bonding surface 14A. The first check valve 24 and the second check valve 25 are raised from the bottoms of the first flow path 22 and the second flow path 23, and are provided integrally with other portions of the soft member 14.

  The nucleic acid detection device 11 connects a plurality of sensors 26 provided in the reaction channel 21, a plurality of detection electrodes 27 provided corresponding to the sensors 26, and a plurality of sensors 26 and a plurality of detection electrodes 27. And a plurality of wirings 28. The sensor 26 and the detection electrode 27 are provided on a substrate 32 (DNA chip). The inspection device 12 includes a determination unit 31 connected to each detection electrode 27. The determination unit 31 can acquire the output value of each sensor 26 via each detection electrode 27. In the present embodiment, the determination unit 31 is included in the inspection apparatus 12 (more specifically, a computer in the inspection apparatus), but the determination unit 31 is included in the concept of “nucleic acid detection device”.

  The plurality of sensors 26 are provided in series in the reaction channel 21. As shown in FIG. 2, the plurality of sensors 26 includes a first sensor group 33 to which a first nucleic acid probe is fixed and a second sensor group 34 to which a second nucleic acid probe different from the first nucleic acid probe is fixed. And an nth sensor group to which an nth nucleic acid probe different from the first nucleic acid probe and the second nucleic acid probe is fixed.

  Each sensor group 33, 34 includes a plurality of sensors 26, and the same nucleic acid probe is fixed to the plurality of sensors 26 included in each sensor group 33, 34. The first sensor group 33 includes, for example, a first sensor 26A, a second sensor 26B, and a third sensor 26C. Each sensor 26 is provided independently of each other. Each sensor 26 can emit a signal toward the detection electrode 27 and the determination unit 31 when the nucleic acid to be detected is bound to the nucleic acid probe.

  As shown in FIG. 2, the plurality of detection electrodes 27 include a first detection electrode group 41 corresponding to the first sensor group 33, a second detection electrode group 42 corresponding to the second sensor group 34, and an nth sensor. N-th detection electrode group corresponding to the group. The first detection electrode group 41 includes, for example, a first detection electrode 27A, a second detection electrode 27B, and a third detection electrode 27C. The first detection electrode 27A is connected to the first sensor 26A via the wiring 28 and can acquire a signal from the first sensor 26A. The second detection electrode 27B is connected to the second sensor 26B via the wiring 28 and can acquire a signal from the second sensor 26B. The third detection electrode 27C is connected to the third sensor 26C via the wiring 28, and can acquire a signal from the third sensor 26C. The second detection electrode group 42 and the nth detection electrode group are also substantially the same as the first detection electrode group 41.

  The distance b between the adjacent sensor groups 33 and 34 is, for example, smaller than three times the distribution width a of the sensor groups 33 and 34, and the sensors 26 (first sensors 26 </ b> A) included in the sensor groups 33 and 34. , Larger than the distance c between adjacent sensors in the second sensor 26B and the third sensor 26C).

  Then, with reference to FIG. 1, the effect | action of the device 11 for nucleic acid detection of this embodiment is demonstrated. As shown in FIG. 1, the specimen sample is supplied into the first syringe 15 </ b> A of the reagent inflow syringe 15 from the outside of the nucleic acid detection device 11 through the injection hole. When the nucleic acid detection device 11 is mounted on the inspection apparatus 12, the pressing mechanism (pressing bar) of the inspection apparatus 12 operates to push the specimen sample in the first syringe 16 into the reaction flow path 21. Enzymes and reagents necessary for DNA amplification are immobilized in the reaction channel 21 in advance. The temperature of the reaction channel 21 is controlled, and the sample sample DNA is amplified by the principle of the LAMP method or the PCR method, for example. After the DNA amplification is completed, the amplified DNA is adsorbed to the sensor 26 in the reaction channel 21. The sensor 26 previously adsorbs a single-stranded DNA (probe) having a complementary sequence that can form a double-stranded strand with the target sequence.

  Subsequently, the pressing mechanism (pressing bar) of the inspection apparatus is operated to push the cleaning liquid in the second syringe 15 </ b> B into the reaction channel 21. As a result, excess DNA not adsorbed by the sensor 26 in the reaction channel 21 is washed. Furthermore, the pressing mechanism (pressing bar) of the inspection device 12 is operated to push the insertion agent in the third syringe 15C into the reaction channel 21. Whether or not double-stranded DNA is formed in each sensor 26 by the intercalating agent, that is, whether or not there is a target DNA sequence, is detected as an electrical signal. Of the liquid discharged from the first syringe 15A to the third syringe 15C, an excessive amount of liquid is discharged to the fluid discharge syringe 16 side.

  Next, the determination in the determination unit 31 will be described with reference to FIG. For example, it is assumed that in the first sensor 26A, the first sensor 26A detects (detects via an intercalating agent) that the nucleic acid to be detected binds to the probe (forms double-stranded DNA). At this time, the first sensor 26A emits an electrical signal, and the determination unit 31 acquires an output value via the first detection electrode 27A. Similarly, when the detection target is coupled to the probe of the second sensor 26B, the determination unit 31 acquires the output value of the second sensor 26B via the second detection electrode 27B. When the detection target is coupled to the probe of the third sensor 26C, the determination unit 31 acquires the output value of the third sensor 26C via the third detection electrode 27C.

  The determination unit 31 compares three output values respectively obtained from the first sensor 26A to the third sensor 26C. Of the three output values, if one output value is different and the other two output values are the same, the other two output values are normal, and one output value with a different value is abnormal Judge.

  In the present embodiment, the areas of the first sensor 26A to the third sensor 26C are all the same, but the area can be changed between one sensor 26 and another sensor 26. In this case, after one output value is corrected (divided) by the area of the sensor 26, it can be compared with another output value similarly corrected (divided) by the area of the sensor 26. For example, when the area of the first sensor 26A is twice the area of the second sensor 26B or the third sensor 26C, the output value of the first detection electrode 27A is divided by 2, so that the second detection electrode 27B or It can be compared with the output value from the third detection electrode 27C. At this time, when the respective values are the same, the determination unit 31 determines that the output value is normal.

  According to the first embodiment, since three output values can be obtained from the three sensors 26, even if one of the output values differs from the other output value, the other two output values It is possible to determine that the output value is normal and the value of the output value itself by the principle of majority voting using. As a result, the reliability of the test can be improved, and the number of sensors 26 and the number of detection electrodes 27 can be reduced as much as possible, whereby the nucleic acid detection device 11 can be miniaturized and highly integrated, and the test time can be shortened. If the nucleic acid detection device 11 is miniaturized and highly integrated, it is possible to reduce the number of reagents necessary for the inspection and to reduce the inspection cost.

  Moreover, according to this embodiment, the distance between adjacent sensor groups 33 and 34 can be made as small as possible. As a result, the nucleic acid detection device 11 can be miniaturized and highly integrated.

  Next, a second embodiment of the nucleic acid detection device will be described with reference to FIG. The nucleic acid detection device 11 of the second embodiment differs from that of the first embodiment in the number of detection electrodes, but the other parts are common to the first embodiment. For this reason, a different part is mainly demonstrated and illustration is abbreviate | omitted or abbreviate | omitted about the part which is common in 1st Embodiment.

  The nucleic acid detection device 11 connects a plurality of sensors 26 provided in the reaction channel 21, a plurality of detection electrodes 27 provided corresponding to the sensors 26, and a plurality of sensors 26 and a plurality of detection electrodes 27. And a plurality of wirings 28. The inspection device 12 includes a determination unit 31 connected to each detection electrode 27. The determination unit 31 can acquire the output value of each sensor 26 via each detection electrode 27. In the present embodiment, the determination unit 31 is included in the inspection apparatus 12 (more specifically, the computer of the inspection apparatus 12), but the determination unit 31 is included in the concept of “nucleic acid detection device”.

  The plurality of sensors 26 are provided in series in the reaction channel 21. The plurality of sensors 26 include a first sensor group 33 to which a first nucleic acid probe is fixed, a second sensor group 34 to which a second nucleic acid probe different from the first nucleic acid probe is fixed, a first nucleic acid probe, and a first nucleic acid probe. And an n-th sensor group to which an n-th nucleic acid probe different from the two nucleic acid probes is fixed.

  Each sensor group 33, 34 includes a plurality of sensors 26, and the same nucleic acid probe is fixed to the plurality of sensors 26 included in each sensor group 33, 34. The areas of the sensors 26 are all the same, for example. The first sensor group 33 includes, for example, a first sensor 26A, a second sensor 26B, and a third sensor 26C. The second sensor group 34 includes a plurality of sensors 26 to which the second nucleic acid probe is fixed. Each sensor 26 is provided independently of each other. Each sensor 26 can emit a signal toward the detection electrode 27 and the determination unit 31 when the nucleic acid to be detected is bound to the nucleic acid probe.

  The plurality of detection electrodes 27 include a first detection electrode group 41 corresponding to the first sensor group 33, a second detection electrode group 42 corresponding to the second sensor group 34, and an nth detection corresponding to the nth sensor group. An electrode group. The first detection electrode group 41 includes, for example, a first detection electrode 27A and a second detection electrode 27B. The first detection electrode 27A is connected to the first sensor 26A and the second sensor 26B via the wiring 28 and can acquire signals from the first sensor 26A and the second sensor 26B. The second detection electrode 27B is connected to the third sensor 26C via the wiring 28, and can acquire a signal from the third sensor 26C. The second detection electrode group 42 and the nth detection electrode group are also substantially the same as the first detection electrode group 41.

  In the present embodiment, the distance b between the adjacent sensor groups 33 and 34 is, for example, smaller than three times the distribution width a of the sensor groups 33 and 34, and the sensors 26 included in the sensor groups 33 and 34. The distance c is larger than the distance c between adjacent sensors 26 in the first sensor 26A, the second sensor 26B, and the third sensor 26C.

  Subsequently, the determination in the determination unit 31 will be described. For example, it is assumed that in the first sensor 26A, the first sensor 26A detects (detects via an intercalating agent) that the nucleic acid to be detected binds to the probe (forms double-stranded DNA). At this time, the first sensor 26A emits an electrical signal, and the determination unit 31 acquires an output value via the first detection electrode 27A. Similarly, when the detection target is coupled to the probe of the second sensor 26B, the determination unit 31 acquires the output value of the second sensor 26B via the first detection electrode 27A. That is, the determination unit 31 acquires a current value (output value) that is twice the current value (output value) of one sensor 26 via the first detection electrode 27A. When the detection target is coupled to the probe of the third sensor 26C, the determination unit 31 acquires the current value (output value) of the third sensor 26C via the third detection electrode 27C.

  The determination unit 31 compares two current values (output values) obtained from the first detection electrode 27A and the second detection electrode 27B, respectively. The determination unit 31 compares the ratio between the two, and determines that there is some detection abnormality when the ratio deviates greatly from 2: 1. The determination unit 31 determines that the output value is normal when the ratio between the two is 2: 1.

  In the present embodiment, as in the first embodiment, the output value obtained from the first detection electrode 27A is corrected (divided) by the sum of the area of the first sensor 26A and the area of the second sensor 26B, for example. Thus, the output value obtained from the third detection electrode 27C can be compared with a value corrected (divided) by the area of the third sensor 26C. At this time, when both values are the same, the determination unit 31 determines that the output value is normal.

  According to the second embodiment, the number of detection electrodes 27 can be reduced, and the nucleic acid detection device 11 can be miniaturized and highly integrated, and the inspection time can be shortened. In addition, the area occupied by the wiring 28 can be reduced to reduce the size of the nucleic acid detection device 11. Furthermore, the reliability of the output value can be improved by examining the ratio of the output values of the first detection electrode 27A and the second detection electrode 27B.

  Moreover, according to this embodiment, the distance between adjacent sensor groups 33 and 34 can be made as small as possible. As a result, the nucleic acid detection device 11 can be miniaturized and highly integrated.

  Next, a third embodiment of the nucleic acid detection device will be described with reference to FIG. The nucleic acid detection device 11 of the third embodiment is different from that of the first embodiment in the number of sensors 26 and detection electrodes 27, but the other parts are the same as those in the first embodiment. For this reason, a different part is mainly demonstrated and illustration is abbreviate | omitted or abbreviate | omitted about the part which is common in 1st Embodiment.

  The nucleic acid detection device 11 connects a plurality of sensors 26 provided in the reaction channel 21, a plurality of detection electrodes 27 provided corresponding to the sensors 26, and a plurality of sensors 26 and a plurality of detection electrodes 27. And a plurality of wirings 28. The inspection device 12 includes a determination unit 31 connected to each detection electrode 27. The determination unit 31 can acquire the output value of each sensor 26 via each detection electrode 27. In the present embodiment, the determination unit 31 is included in the inspection apparatus 12 (more specifically, the computer of the inspection apparatus 12), but the determination unit 31 is included in the concept of “nucleic acid detection device”.

  The plurality of sensors 26 are provided in series in the reaction channel 21. The plurality of sensors 26 include a first sensor group 33 corresponding to the first nucleic acid probe, a second sensor group 34 to which a second nucleic acid probe different from the first nucleic acid probe is fixed, a first nucleic acid probe, and An n-th sensor group to which an n-th nucleic acid probe different from the second nucleic acid probe is fixed.

  Each sensor group 33, 34 includes a plurality of sensors 26, and the same nucleic acid probe is fixed to the plurality of sensors 26 included in each sensor group 33, 34. The areas of the sensors 26 are all the same, for example. Each sensor group 33, 34 includes, for example, 5 or more and 10 or less sensors 26. Each sensor 26 can emit a signal toward the detection electrode 27 and the determination unit 31 when the nucleic acid to be detected is bound to the nucleic acid probe.

  The plurality of detection electrodes 27 include a first detection electrode group 41 corresponding to the first sensor group 33, a second detection electrode group 42 corresponding to the second sensor group 34, and an nth detection corresponding to the nth sensor group. An electrode group. Each of the detection electrode groups 41 and 42 further includes a plurality of detection electrodes 27. Each detection electrode group 41, 42 includes two or more detection electrodes 27 that do not exceed the number of sensors 26.

  In each sensor group 33, 34 and each detection electrode group 41, 42, two sensors 26 are connected to one detection electrode 27, or one sensor 26 is connected to one detection electrode 27. ing. The same applies to the second sensor group 34 and the n-th sensor group.

  In the present embodiment, the distance b between the adjacent sensor groups 33 and 34 is, for example, smaller than three times the distribution width a of the sensor groups 33 and 34, and the sensors 26 included in the sensor groups 33 and 34. The distance c is greater than the distance c between adjacent sensors 26.

  Subsequently, the determination in the determination unit 31 will be described. For example, in one sensor 26, it is assumed that the nucleic acid to be detected is bound to the probe (double-stranded DNA is formed), and the sensor 26 detects (detects via an intercalating agent). At this time, the sensor 26 emits an electrical signal, and the determination unit 31 acquires an output value via the detection electrode 27. Similarly, when the detection target is coupled to the probe of another one sensor 26, the determination unit 31 acquires the output value of the sensor 26 via the detection electrode 27. In the present embodiment, there are places where two sensors 26 are connected to one detection electrode 27, and in this case, the determination unit 31 uses two detection electrodes 27 to connect the two sensors 26. Get the output value. In other places, one sensor 26 is connected to one detection electrode 27.

  The determination unit 31 compares a plurality of output values respectively obtained from the detection electrodes 27. When the output values are different from each other among the plurality of output values, it is determined that the output values occupying the majority are normal and the small number of output values having different values are abnormal. The output value acquired from one detection electrode 27 connected to the two sensors 26 is compared with the output value occupying the majority. When the output value acquired from one detection electrode 27 connected to the two sensors 26 is an exact double value of the output value occupying the majority, the determination unit 31 outputs the output occupying the majority. It is determined that the value is normal. When the output value acquired from one detection electrode 27 connected to the two sensors 26 does not become twice the output value occupying the majority, the determination unit 31 determines that there is some detection abnormality. judge.

  In addition, the connection method and determination method of the sensor 26 and the detection electrode 27 described above are examples, and the following modifications exist in these connection methods and determination methods. In these modifications, the number of sensors 26 connected to one detection electrode 27 depends on the number of sensors 26 included in each sensor group 33 and the number of detection electrodes 27 included in each detection electrode group 41, 42. Is different.

  If the number of sensors 26 included in each sensor group 33 is five, the number of detection electrodes 27 included in each detection electrode group 41, 42 is two, three, four, or five. Become.

  In the first modification, for example, there are two detection electrodes 27 included in each of the detection electrode groups 41 and 42, and the sensor 26 is connected to the detection electrodes 27 in the following pattern. In the first pattern, four sensors 26 are connected to one detection electrode 27, and one sensor 26 is connected to the other detection electrode 27. In the second pattern, three sensors 26 are connected to one detection electrode 27, and two sensors 26 are connected to the other detection electrode 27.

  In the first pattern, as in the first embodiment, the output value obtained from one detection electrode 27 is corrected (divided) by the sum of the areas of the four sensors 26, for example, so that the other detection electrode It can be compared with the output value obtained from 27. At this time, when both values are the same, the determination unit 31 determines that the output value is normal. As a result, high reliability of inspection can be realized.

  In the second pattern, as in the first embodiment, the output value obtained from one detection electrode 27 is corrected (divided) by the sum of the areas of the three sensors 26, for example, so that the other detection electrode The output value obtained from 27 can be compared with the value corrected (divided) by the area of the two sensors 26. At this time, when both values are the same, the determination unit 31 determines that the output value is normal. As a result, high reliability of inspection can be realized.

  In the second modification, for example, there are three detection electrodes 27 included in each of the detection electrode groups 41 and 42, and the sensor 26 is connected to the detection electrodes 27 in the following pattern. In the first pattern, three sensors 26 are connected to the first detection electrode 27, one sensor 26 is connected to the second detection electrode 27, and one sensor 26 is connected to the third detection electrode 27. Connected. In the second pattern, two sensors 26 are connected to the first detection electrode 27, two sensors 26 are connected to the second detection electrode 27, and one sensor 26 is connected to the third detection electrode 27. Connected.

  In the first pattern, as in the first embodiment, the output value obtained from the first detection electrode 27 is corrected (divided) by, for example, the sum of the areas of the three sensors 26 to obtain the second value. The output values obtained from the detection electrode 27 and the third detection electrode 27 can be compared. The determination unit 31 compares three output values obtained from each of the first detection electrode 27 to the third detection electrode 27. Of the three output values, if one output value is different and the other two output values are the same, the other two output values are normal, and one output value with a different value is abnormal Judge. As a result, high reliability of inspection can be realized.

  In the second pattern, similarly to the first embodiment, the output values obtained from the first detection electrode 27 and the second detection electrode 27 are corrected (divided) by the sum of the areas of the two sensors 26, for example. By doing so, it can be compared with the output value obtained from the third detection electrode 27. The determination unit 31 compares three output values obtained from each of the first detection electrode 27 to the third detection electrode 27. Of the three output values, if one output value is different and the other two output values are the same, the other two output values are normal, and one output value with a different value is abnormal Judge. As a result, high reliability of inspection can be realized.

  In the third modification, for example, there are four detection electrodes 27 included in each of the detection electrode groups 41 and 42, and they are connected as follows. That is, two sensors 26 are connected to the first detection electrode 27, one sensor 26 is connected to the second detection electrode 27, and one sensor 26 is connected to the third detection electrode 27, One sensor 26 is connected to the fourth detection electrode 27.

  In this case, similarly to the first embodiment, the output value obtained from the first detection electrode 27 is corrected (divided) by, for example, the sum of the areas of the two sensors 26 to obtain the second detection electrode. 27 to the output value obtained from the fourth detection electrode 27 can be compared. The determination unit 31 compares four output values obtained from each of the first detection electrode 27 to the fourth detection electrode 27. Of the four output values, when one output value is different and the other three output values are the same, the other three output values are normal and one output value with a different value is abnormal Judge. As a result, high reliability of inspection can be realized.

  In the fourth modification, for example, there are five detection electrodes 27 included in each of the detection electrode groups 41 and 42, and they are connected as follows. That is, one sensor 26 is connected to the first detection electrode 27, one sensor 26 is connected to the second detection electrode 27, and one sensor 26 is connected to the third detection electrode 27, One sensor 26 is connected to the fourth detection electrode 27, and one sensor 26 is connected to the fifth detection electrode 27.

  In this case, the determination unit 31 compares the five output values obtained from each of the first detection electrode 27 to the fifth detection electrode 27. Of the five output values, if one output value is different and the other four output values are the same, the other four output values are normal and one output value with a different value is abnormal Judge. As a result, high reliability of inspection can be realized.

  According to the third embodiment, since the number of sensors 26 corresponding to the same probe is 5 or more and 10 or less, the reliability of the inspection can be improved.

  In addition, it goes without saying that the nucleic acid detection device can be variously modified and implemented without departing from the gist of the invention.

DESCRIPTION OF SYMBOLS 11 ... Nucleic acid detection device, 26 ... Sensor, 26A ... 1st sensor, 26B ... 2nd sensor, 26C ... 3rd sensor, 27 ... Detection electrode, 27A ... 1st detection electrode, 27B ... 2nd detection electrode, 27C ... 3rd detection electrode, 31 ... determination part, 33 ... 1st sensor group, 34 ... 2nd sensor group

Claims (8)

A first sensor that emits a signal when a probe is immobilized and a nucleic acid to be detected is bound to the probe;
A second sensor which is provided independently of each of the first sensors and which emits a signal when the same probe as the probe fixed to the first sensor is fixed and a nucleic acid to be detected is bound to the probe; 3 sensors,
A first detection electrode, a second detection electrode, and a third detection electrode that obtain signals from the first sensor, the second sensor, and the third sensor;
A determination unit that is connected to the first detection electrode, the second detection electrode, and the third detection electrode, and that respectively obtains output values of signals from the first sensor, the second sensor, and the third sensor; ,
With
The first sensor is connected to the first detection electrode;
The second sensor is connected to the second detection electrode;
The third sensor is connected to the third detection electrode;
The determination unit compares the output value extracted from the first detection electrode, the output value extracted from the second detection electrode, and the output value extracted from the third detection electrode, so that the output value is normal. Nucleic acid detection device for determining sex.   The determination unit corrects an output value extracted from the first detection electrode by an area of the first sensor, corrects an output value extracted from the second detection electrode by an area of the second sensor, and The nucleic acid detection device according to claim 1, wherein the output value taken out from the detection electrode is corrected by the area of the third sensor, and the corrected output value is compared to determine the normality of the output value. A first sensor that emits a signal when a probe is immobilized and a nucleic acid to be detected is bound to the probe;
A second sensor which is provided independently of each of the first sensors and which emits a signal when the same probe as the probe fixed to the first sensor is fixed and a nucleic acid to be detected is bound to the probe; 3 sensors,
A first detection electrode and a second detection electrode that obtain signals from the first sensor, the second sensor, and the third sensor;
A determination unit that is connected to the first detection electrode and the second detection electrode, and that respectively obtains output values of signals from the first sensor, the second sensor, and the third sensor;
With
The first sensor and the second sensor are connected to the first detection electrode,
The third sensor is connected to the second detection electrode;
The determination unit compares the output value extracted from the first detection electrode with the output value extracted from the second detection electrode, and determines the normality of the output value.   The determination unit corrects an output value extracted from the first detection electrode by a sum of areas of the first sensor and the second sensor, and determines an output value extracted from the second detection electrode as an area of the third sensor. The nucleic acid detection device according to claim 3, wherein the normality of the output value is determined by comparing the output values after correction and comparing the corrected output values. A first sensor group including the first sensor, the second sensor, and the third sensor;
A second sensor group including a plurality of sensors to which probes different from the probes fixed to the third sensor from the first sensor are fixed;
With
The distance between the first sensor group and the second sensor group is smaller than three times the distribution width of the first group, and is adjacent in the first sensor, the second sensor, and the third sensor. The nucleic acid detection device according to claim 2 or 4, wherein the device is larger than a distance between the sensors. 5 or more and 10 or less sensors that emit a signal when the same probe is immobilized and the nucleic acid to be detected binds to the probe;
A plurality of detection electrodes for acquiring signals of the sensors;
A determination unit that is connected to the plurality of detection electrodes and that acquires an output value of a signal from each of the sensors;
With
The determination unit is a nucleic acid detection device that determines the normality of an output value by comparing output values extracted from the detection electrodes.   The determination unit corrects an output value extracted from a first detection electrode in the plurality of detection electrodes by a sum of areas of the plurality of sensors connected to the first detection electrode, and the plurality of detection electrodes The output value taken out from the second detection electrode is corrected with the sum of the areas of the plurality of sensors connected to the second detection electrode, and taken out from the third detection electrode in the plurality of detection electrodes The output value is corrected by a sum of areas of the plurality of sensors connected to the third detection electrode, and the normality of the output value is determined by comparing these corrected output values. Nucleic acid detection device. A first sensor group including 5 or more and 10 or less sensors;
A second sensor group including a plurality of sensors to which probes different from the probes fixed to 5 or more and 10 or less sensors are fixed;
With
The distance between the first sensor group and the second sensor group is smaller than three times the distribution width of the first group, and between adjacent sensors among the five to ten sensors. The nucleic acid detection device according to claim 7, wherein the nucleic acid detection device is larger than the distance.

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