JP2014028779A - Grape insect pest extermination formulation and grape insect pest extermination method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an insect pest extermination formulation that is excellent in safety and can obtain a high insect pest extermination effect.SOLUTION: A solid grape insect pest extermination formulation includes: an insecticidal component having a hard volatilization property at an atmospheric temperature; and a sublimable substance that has an easier volatilization property than that of the insecticidal component at an atmospheric temperature, and in the grape insect pest extermination formulation, a volume reduces with time or a form is broken with time by sublimation of the sublimable substance, a content of the insecticidal component in the formulation does not substantially reduce with time, and a concentration of the insecticidal component in the formulation increases with a balance change of a volume of the formulation and a content of the insecticidal component.

Description

  The present invention relates to a moth pest control formulation and a method for controlling moth pests, and in particular, to a moth pest control formulation that exhibits excellent safety and a high pest control effect, and a method for controlling worm pests using the preparation .

In conventional pest control formulations (sprays, etc.), it is difficult to process an effective amount of an insecticidal component against pests that cannot be directly treated with chemicals, such as pests lurking in the gaps and refrigerators. The chemical | medical agent was processed to the back of the clearance gap by providing a nozzle, increasing a propellant so that a chemical | medical solution may reach far, and injecting in large quantities (refer patent documents 1-3). However, when a large amount of aerosol is injected, flammable gas is contained, and there is a danger of igniting if treated in large quantities. In some cases, there were safety issues such as inhaling scattered medicine.
In addition, a method of applying a lethal amount of an insecticidal component that can be surely controlled with a single contact to a place where a pest is likely to pass is considered. There were also safety issues such as the possibility of direct contact with the drug. In addition, there is a problem that the interior of the room becomes dirty by treating a large amount of a chemical solution containing a solvent.

JP 2004-26278 A JP-A-8-259404 International Publication No. 2005/013685

  In view of the above-described conventional problems, an object of the present invention is to provide a moth pest control preparation which is excellent in safety and has a high pest control effect.

  As a result of earnest study, the present inventors have found that the above object can be achieved by the following constitution, and have completed the present invention.

That is, the present invention relates to the following (1) to (4).
(1) A solid insect pest control formulation comprising a non-volatile insecticidal component at room temperature and a sublimable substance that is more easily volatilized than the insecticidal component at room temperature,
The preparation is one whose volume decreases with time due to sublimation of the sublimable substance or whose shape collapses with time.
The content of the insecticidal component in the preparation is substantially not reduced over time,
A pest control formulation in which the concentration of the insecticidal component in the formulation increases as the balance between the volume of the formulation and the content of the insecticidal component changes.
(2) The pest control agent according to (1), wherein the sublimable substance is at least one selected from isopropyltrioxane, adamantane, menthol, naphthalene, camphor, and paradichlorobenzene.
(3) The pest control agent according to (1) or (2) above, which contains at least one selected from stearates, talc, titanium oxide, paraffin, and polyhydric alcohols as a lubricant.
(4) A solid insect pest control formulation comprising a non-volatile insecticidal component that is difficult to volatilize at room temperature and a sublimable substance that is more easily volatilized than the insecticidal component at room temperature, and the volume is reduced by sublimation of the sublimable substance. Insect pest control, a solid moth pest control formulation that increases in concentration as the amount of the insecticidal component increases and the balance of the volume of the formulation and the content of the insecticidal component changes as the volume decreases over time or the shape collapses over time A pest control method to be installed at the site.

  According to the present invention, it exerts an excellent controlling effect against pests, particularly moth pests, and further, even when charged with a low concentration of the insecticidal component at the time of preparation, the concentration of the insecticidal component in the outer layer of the formulation over time, It is possible to provide a pest control agent that is capable of exposing a lethal dose of a pest to a pest and is excellent in safety and handling.

The pest control formulation of the present invention (hereinafter also referred to as “the formulation of the present invention”) contains a non-volatile insecticidal component at room temperature and a sublimable substance that is easier to evaporate at room temperature than the insecticidal component. The pest control mechanism by the preparation is not clear, but when the sublimable substance is sublimated, the insecticidal component of the preparation is concentrated over time, so that the insect body adheres to the insect body or after the attachment. The concentration of the insecticidal component on the surface becomes the same as that of the drug itself, and it is presumed that the pest is killed through ingestion by grooming behavior in addition to the action of the drug directly contacting the body surface.
Therefore, the preparation of the present invention is highly safe and easy to handle because a high pest control effect can be obtained even if the concentration of the insecticidal component used at the preparation of the preparation is low.

The insecticidal component contained in the preparation of the present invention may be any insecticidal component that is hardly volatile at room temperature. Here, “non-volatile” means that the vapor pressure at 25 ° C. is less than 1.0 × 10 ⁻⁴ Pa. If it is less than 1.0 × 10- 4 ^Pa vapor pressure at 25 ° C., the content of the insecticidal ingredient in the formulation does not substantially decrease. Here, “substantially not reduced” means that the amount of reduction at room temperature for 2 weeks is 5% or less. If the insecticidal component is non-volatile, the concentration of the insecticidal component in the preparation is increased by sublimating the sublimable substance that plays the role of the carrier first. Specific examples include insecticidal components such as dinotefuran, permethrin, imiprothrin, bifenthrin and the like. One insecticide component may be used alone, or two or more insecticide components may be used in combination. Moreover, it is preferable that an insecticidal component is 0.001 mass%-20 mass% with respect to the mass of the whole preparation, and it is more preferable that it is 0.001 mass%-10 mass%.

  In addition, the sublimable substance contained in the preparation of the present invention is not limited as long as it is more easily vaporized at room temperature than the insecticidal component, and preferably has a higher vapor pressure than the insecticidal component. Name “Sunsaburi B” (manufactured by Ogawa Fragrance Co., Ltd.), adamantane, neopentyl glycol, paradichlorobenzene, naphthalene, camphor, menthol, hydroquinone and the like. Among these, propyltrioxane, adamantane, and menthol are preferable from the viewpoint of low irritation and high safety to the body. Furthermore, when used as a tablet, isopropyltrioxane and adamantane are particularly preferable in terms of high formability. Moreover, it is preferable that the vapor pressure of the sublimable substance is 10 Pa or more higher than the vapor pressure of the insecticidal component.

The content of the sublimable substance is preferably 50% by mass to 99.999% by mass and more preferably 70% by mass to 99.99% by mass with respect to the mass of the entire preparation.
These sublimable substances also have a function of improving the adherence of the preparation to the insect pest surface.

  Examples of the target pests to be controlled by the preparation of the present invention include agricultural pests, stored grain pests, hygiene pests, and unpleasant pests. More specifically, cockroaches, bed bugs, centipedes, millipedes, dandelions and the like can be mentioned. Above all, it exhibits a particularly high control effect against worms such as cockroaches.

  The preparation of the present invention is a solid preparation. The solid dosage form is preferable because it is highly convenient during production and handling. Specific examples include tableting tablets, pills, powders, granules, and the like arranged in a certain shape. Among these, tableting tablets and granules are preferable, and stationary tableting installed in a place where pests can live is more preferable.

  In the present preparation, as the sublimable substance is sublimated, the volume of the preparation decreases with time or the shape of the preparation collapses with time. On the other hand, since the insecticidal component is hardly volatile at room temperature, the content in the preparation does not substantially decrease with time. As a result, the concentration of the insecticidal component in the preparation increases with time as the balance between the volume of the preparation and the content of the insecticidal component changes. Therefore, even if the concentration of the insecticidal component at the time of preparation is low enough not to cause the pest to die, it adheres to the surface of the insect pest and increases to the same level as the concentration of the insecticidal component drug substance. In addition to the effect of directly contacting the body surface of the pest, the preparation can be sufficiently ingested by a grooming action to sufficiently obtain a pest control effect. It is considered that the concentration of the insecticidal component in the preparation is expressed at least in the outer layer of the preparation and gradually proceeds inside the preparation.

In addition, as the sublimable substance sublimates, the volume of the preparation decreases with time, or the shape of the preparation collapses with time, and thus the preparation is easily miniaturized. It is considered that the sublimable substance that has not yet sublimated yet acts as a spreading agent on the micronized preparation, and the insecticidal component easily adheres to the surface of the worm body.
In the preparation adhered to the surface of the insect body, the concentration of the insecticidal component is further increased by sublimation of the sublimable substance, and the insect body itself propagates the insecticidal component by the grooming of the insect body itself or contact with other insect bodies.
Although the insecticidal component adhering to the worm body is not certain, there is a route through which it can be taken orally and lead to the death of the pest in addition to direct contact.

  The preparation of the present invention further includes additives such as magnesium stearate, calcium stearate, talc, sodium stearyl fumarate, stearic acid, paraffin, carnauba wax, polyhydric alcohol, light anhydrous silicic acid, hydrous silicon dioxide, and a lubricant. You may mix | blend 1 or more types. By containing these, the formability, stability, processability and adhesion to pests of the preparation are improved. The content in these preparations is preferably 0.1 to 2.0% by mass.

  In particular, magnesium stearate plays a role as a shape-retaining agent of the preparation, and can be expected to prevent premature disintegration of the preparation due to sublimation of the sublimable substance. As a result, the concentration of the insecticidal component in the outer layer of the preparation is sufficiently high for pest control, and it is possible to have an effect maintenance period as necessary, and it is more preferable because it exerts a pest control effect for a certain period of time. .

  In the preparation of the present invention, in addition to the above-mentioned components, for example, a shape-retaining agent, a spreading agent, a coating agent, an excipient, a binder, an anticorrosive agent, a stabilizer, an adsorbent, a fragrance, a coloring agent, etc. These various additives may be appropriately blended.

  EXAMPLES The present invention will be described in detail below with reference to examples, but the present invention is not limited to these examples.

<Examples 1-1 to 1-12>
Based on the following conditions, the type of the sublimable substance was changed by methods 1 to 4, and the pest control effect was confirmed.
Formulation formulation (10 g);
Dinotefuran (insecticidal component) 1.0% by mass
Magnesium stearate 0.5% by mass
98.5% by mass of sublimable substances
As the sublimable substance, isopropyltrioxane, adamantane, menthol, naphthalene, camphor, and paradichlorobenzene were used.

(Method 1)
Dinotefuran, magnesium stearate and each sublimable substance were mixed, and a tablet (0.3 g) having a thickness of 6 mm and a diameter of 9 mm was formed using a hydraulic pump and a mold at a pressure of 300 kg · f / m ² .
(Method 2)
A cockroach shelter, solid feed, and water were installed in a plastic case (18 cm long × 25 cm wide × 10 cm high) serving as a test space.
(Method 3)
A total of 20 susceptible German cockroaches, 10 males and 10 males, and 5 black and white cockroaches, males and females, were released into each test space as test worms, and acclimated for 1 day under conditions of a test space temperature of 25 ° C and humidity of 60%. The test was started by placing a tablet as a specimen between solid feed and water. As control test plots, test plots with only solid feed and water were provided for each sensitive German cockroach and black cockroach.
(Method 4)
The number of knockdowns and the number of lethals were counted over time, and the lethality (%) (including knockdown) was calculated by the following formula (1).
Specimen mortality rate = (lethal number / total number of test insects) x 100 (1)
Table 1 shows the results of the efficacy test for each sublimable substance used.

  According to Table 1, although the difference in lethal effect was observed one day after the specimen was installed due to the difference in sublimable substances, all specimens were observed two days after the specimen was installed, regardless of the type of pest or the sublimable substance. Showed a high mortality rate of over 90%.

  From the above, it was confirmed that all of the preparations of the present invention are effective in controlling pests, although there are differences in the time required to develop the effect as a drug depending on the type of sublimable substance.

<Examples 2-1 to 2-6 and Comparative Examples 2-1 to 2-2>
Based on the following conditions, the insect pest control effect when using a sublimable substance or a non-sublimable substance as a base was confirmed by methods 1 to 4.
Formulation formulation (0.7 g):
Dinotefuran (insecticidal component) Content shown in Table 2 (mass ppm)
Base Remaining As the base, isopropyltrioxane, which is a sublimation substance, or calcium silicate (trade name: Florite RN), which is a non-sublimation substance, was used.

(Method 1)
Dinotefuran and each base were mixed, and a tablet (0.7 g) having a thickness of 3 mm and a diameter of 20 mm was formed using a hydraulic pump and a mold at a pressure of 300 kg · f / m ² .
(Method 2)
A paper shelter, solid feed, and water were placed in a plastic case (vertical 18 cm × width 25 cm × height 10 cm) serving as a test space.
(Method 3)
A total of 10 susceptible cockroach males and 5 males and 5 male and female black cockroaches were released as test insects in each test space, and acclimated for 1 day under conditions of a test space temperature of 25 ° C. and a humidity of 60%. The test was started by placing a tablet tablet as a specimen between solid feed and water. As control test plots, test plots with only solid feed and water were provided in each of the sensitive German cockroaches and black cockroaches.
(Method 4)
The number of knockdowns and the number of lethals were counted over time, and the lethality (%) was calculated by the same formula (1) as in Example 1-1.
The results are shown in Table 2.

  From the above, when a non-sublimable substance is used as a base, the concentration of the insecticidal component at which no pest control effect can be obtained is high by using the sublimable substance as a base in the preparation of the present invention. It was confirmed that the extermination effect was obtained.

<Examples 3-1 to 3-4 and Comparative Examples 3-1 to 3-2>
Based on the following conditions, the disintegration property of the preparation in the preparation was confirmed by methods 1 to 3.
Formulation formulation (100 g):
Dinotefuran (insecticidal component) 1.0% by mass
Magnesium stearate 0.5% by mass or none Isopropyltrioxane (sublimable substance) 98.5% by mass or 99.0% by mass

(Method 1)
Insecticidal ingredients, sublimable substance (isopropyltrioxane), and magnesium stearate as necessary are mixed, and using a hydraulic pump and a mold, the pressure is 300 kg · f / m ² with a thickness of 6 mm and a diameter of 9 mm. Tablets (0.3 g) were formed.
(Method 2)
In order to make it easy to observe the appearance of the preparation, a black drawing paper is placed on the bottom of the plastic cup, and one tablet is placed on the paper, and then statically placed at 25 ° C. for 24 days and 30 ° C. for 30 days. The weight loss of the preparation was observed over time.
(Method 3)
The estimated duration in 0.3 g of the preparation was determined from the change in the weight of the obtained preparation (average reduction amount obtained by conducting the same test three times) according to the following formula. The results are shown in Table 3.
Estimated duration at 0.3 g = weight of sublimable substance / average daily reduction

  From Table 3, from the results of Example 3-3 and Comparative Example 3-1, and Example 3-4 and Comparative Example 3-2, the sublimable substance alone even when the insecticidal component was mixed with the sublimable substance. It can be seen that the average decrease in the formulation does not change. In addition, from the results of Examples 3-1 to 3-4, even when magnesium stearate is mixed with a preparation containing an insecticidal component and a sublimable substance, there is no significant difference in the amount of reduction and the duration of the preparation. It was confirmed.

<Example 4, comparative example 4>
Based on the following conditions, the change in insecticidal component concentration over time was confirmed for the preparation.
Example 4: Formulation formulation (100 g);
Dinotefuran (insecticidal component) 10.0% by weight
Magnesium stearate 0.5% by weight
Isopropyltrioxane (sublimable substance) 89.5% by weight
Comparative Example 4: Formulation formulation (100 g);
Dinotefuran (insecticidal component) 10.0% by weight
Calcium silicate (non-sublimable substance) 90.0% by weight
The above formulation was mixed to produce a tablet (7 g) having a diameter of 20 mm and a height of 10 mm. The tablet was placed in a constant temperature room at 40 ° C. The weight of the preparation was measured at regular intervals, and the insecticidal component content was measured under the following predetermined conditions. The insecticidal component concentration was calculated from the insecticidal component content and the formulation weight. Table 4 shows the average results of three similar tests.

<Insecticide content analysis method>
-The whole preparation (equivalent to about 700 mg of dinotefuran) was pulverized and placed in a sample bottle.
-40 ml of methanol was added to the sample bottle, and ultrasonic extraction was performed for 1 hour.
-As an internal standard, 5 ml of 4-aminobenzoic acid was added and stirred.
(80 mg / 5 mL methanol solution)
A portion was collected and diluted 100 times with mobile phase.
-The sample was filtered through a membrane filter having a pore size of 0.2 µm to obtain a sample for analysis.
-HPLC analysis was performed under the following conditions using a solution obtained by adding 10 mM potassium dihydrogen phosphate to a mixed solvent of ultrapure water: acetonitrile = 93: 7 as a mobile phase.

(HPLC analysis conditions)
Analytical instrument: Shimadzu HPLC LC-3000
Detector: UV (Measurement wavelength: 270 nm)
Flow rate: 1.0 mL / min
Injection volume: 1 μL
Analysis column: Inertsil ODS φ4.0 × 250 mm (manufactured by GL Sciences Inc.)

  As shown in Table 4, in contrast to Example 4 using isopropyltrioxane (sublimation substance), the formulation of Comparative Example 4 using calcium silicate (non-sublimation substance) was an insecticidal component even after 35 days. No change in concentration over time could be confirmed.

<Example 5-1>
Two tablets (pesticide component concentration: 1.0% by mass) prepared in the same manner as in Example 1-1 were placed in a space of 8.8 m ² and a total of 20 male and female cockroach cockroach males and females were used. The head was released into the test space, the change in lethal effect over time was confirmed, and the corrected lethality corrected from the natural death value (control lethality) was calculated using the above formula (1) and the following formula (2). .
Specimen mortality rate = (lethal number / total number of test insects) x 100 (1)
Corrected lethality = (specimen lethality−control lethality) / (1−control lethality) (2)
The corrected lethality rate in equation (2) can be calculated using a control lethality rate based on the lethality rate due to the natural death of the specimen.

<Example 5-2>
A tablet with an insecticidal component concentration of 1/10 (0.1% by mass) with respect to Example 5-1 was placed in the same space as the tablet 10 times as many as Example 5-1 (20 tablets). In the same manner as in Example 5-1, the change over time in the lethal effect on the test insect, German cockroach was confirmed, and the corrected lethality was calculated.

  The results of Examples 5-1 and 5-2 are shown in Table 5.

  From Table 5, when the insecticidal component concentration in the preparation is reduced to 1/10 and the number of installed preparations is 10 times, the time until the extermination effect appears is delayed. On the other hand, since there is no difference in lethality after 3 days from the installation of the preparation, even if the insecticidal ingredient at the time of preparation preparation is a low concentration preparation, by increasing the number of preparations to install, However, it was confirmed that the same disinfecting effect as that of the high concentration preparation was obtained. From this, it can be seen that the preparation according to the present invention can exert a sufficient effect on pest control because the insecticide in the outer layer of the preparation is concentrated by sublimation of the sublimable substance even if the concentration of the insecticide is low.

<Examples 6-1 and 6-2>
Based on the following conditions, the pest control effect was confirmed by methods 1-2.
Formulation formulation (100 g);
Dinotefuran (insecticidal component) 1.0% by mass
Isopropyltrioxane (sublimable substance) 99.0% by mass

(Method 1)
Dinotefuran and a sublimable substance were mixed, and a tablet (0.3 g) having a thickness of 6 mm and a diameter of 9 mm was formed using a hydraulic pump and a mold at a pressure of 300 kg · f / m ² .
(Method 2)
One tablet or five tablets obtained by method 1 were placed in a space of 8.8 m ² , and the change over time of the lethal effect against black wagtails as test insects was confirmed. The above formula of Example 5-1 Using (2), the corrected lethality corrected from the value of natural death was calculated. The results are shown in Table 6.

  Moreover, it can be seen from Table 6 that the immediate effect on pests is improved by increasing the number of preparations to be installed. On the other hand, it was confirmed that even if the number of the preparations was small, a sufficient pest control effect could be obtained although it took time to eliminate the pests.

<Comparative Example 5>
Based on the following conditions, the pest control effect was confirmed by methods 1-3.
Formulation (100mL)
Dinotefuran (insecticidal component) 7.5g
Methanol (solvent) appropriate amount

(Method 1)
1 mL of the preparation was applied to a vinyl chloride plate having a length of 5 cm, a width of 5 cm, and a thickness of 2 mm, and methanol was air-dried to prepare a specimen.
(Method 2)
A wooden box with a paper shelter (length 25 cm x width 35 cm x height 25 cm, with an opening 1 cm in height and 5 cm in width provided in part) in a 1 m ² bat. Twenty-five German cockroaches were placed and acclimated for one day under the condition of 25 ° C., and then a specimen was placed.
(Method 3)
Over time, the knock-down (KD) number and the lethal number of the test insects were counted, and the corrected lethality corrected from the natural death value was calculated using the formula (2) used in Example 5-1. .
The results are shown in Table 7.

  From the results in Table 7, it can be seen that when the sublimable substance is not used as the base containing the insecticidal component, the lethality is not improved over time. In Example 6-1 (one tablet installed) containing a sublimable substance and having the same insecticidal component content, the change over time in the lethal effect was confirmed as described above.

<Prescription example>
Table 8 below shows formulation examples (total 100 g) of the pest control formulation of the present invention.

Claims (4)

A solid insect pest control formulation comprising a non-volatile insecticidal component at room temperature and a sublimable substance that is easier to evaporate than the insecticidal component at room temperature,
The preparation is one whose volume decreases with time due to sublimation of the sublimable substance or whose shape collapses with time.
The content of the insecticidal component in the preparation is substantially not reduced over time,
A pest control formulation in which the concentration of the insecticidal component in the formulation increases as the balance between the volume of the formulation and the content of the insecticidal component changes.   The pesticidal insecticide according to claim 1, wherein the sublimable substance is at least one selected from isopropyltrioxane, adamantane, menthol, naphthalene, camphor, and paradichlorobenzene.   The pest control formulation according to claim 1 or 2, comprising at least one selected from stearates, talc, titanium oxide, paraffin, and polyhydric alcohols as a lubricant.   A solid pest-control product comprising a non-volatile insecticidal component that is difficult to volatilize at room temperature and a sublimable substance that is more easily volatilized than the insecticidal component at room temperature, and the volume of the insecticide increases by time due to sublimation of the sublimable substance. Decrease or shape collapses over time, and a solid moth pest control formulation that increases the concentration of the insecticidal component as the balance between the volume of the formulation and the content of the insecticidal component increases is installed in a place where pests can live How to control moth pests.