JP2013542713A5 - - Google Patents
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- Publication number
- JP2013542713A5 JP2013542713A5 JP2013521142A JP2013521142A JP2013542713A5 JP 2013542713 A5 JP2013542713 A5 JP 2013542713A5 JP 2013521142 A JP2013521142 A JP 2013521142A JP 2013521142 A JP2013521142 A JP 2013521142A JP 2013542713 A5 JP2013542713 A5 JP 2013542713A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- reaction vessel
- polymerase
- dna polymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004707 nucleic acids Proteins 0.000 claims 40
- 150000007523 nucleic acids Chemical class 0.000 claims 40
- 102000039446 nucleic acids Human genes 0.000 claims 40
- 238000000034 method Methods 0.000 claims 18
- 230000003321 amplification Effects 0.000 claims 12
- 238000003199 nucleic acid amplification method Methods 0.000 claims 12
- 102100034343 Integrase Human genes 0.000 claims 10
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 10
- 230000000694 effects Effects 0.000 claims 10
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 8
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 8
- 239000007788 liquid Substances 0.000 claims 8
- 239000003153 chemical reaction reagent Substances 0.000 claims 7
- 239000000463 material Substances 0.000 claims 7
- 239000007787 solid Substances 0.000 claims 6
- 241000589596 Thermus Species 0.000 claims 4
- 238000000926 separation method Methods 0.000 claims 4
- 238000013518 transcription Methods 0.000 claims 4
- 230000035897 transcription Effects 0.000 claims 4
- 230000035772 mutation Effects 0.000 claims 3
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims 1
- 108010017826 DNA Polymerase I Proteins 0.000 claims 1
- 102000004594 DNA Polymerase I Human genes 0.000 claims 1
- 108010076551 DNA polymerase C Proteins 0.000 claims 1
- 101000902592 Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) DNA polymerase Proteins 0.000 claims 1
- 241000206213 Thermosipho africanus Species 0.000 claims 1
- 241000204666 Thermotoga maritima Species 0.000 claims 1
- 241000204664 Thermotoga neapolitana Species 0.000 claims 1
- 241000589501 Thermus caldophilus Species 0.000 claims 1
- 241000589498 Thermus filiformis Species 0.000 claims 1
- 108010085671 Thermus thermophilus DNA polymerase Proteins 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
- 239000011534 wash buffer Substances 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36896610P | 2010-07-29 | 2010-07-29 | |
| US61/368,966 | 2010-07-29 | ||
| EP10175545.2 | 2010-09-07 | ||
| EP10175545 | 2010-09-07 | ||
| PCT/EP2011/062957 WO2012013732A1 (en) | 2010-07-29 | 2011-07-27 | Generic pcr |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013542713A JP2013542713A (ja) | 2013-11-28 |
| JP2013542713A5 true JP2013542713A5 (https=) | 2014-05-22 |
| JP5972265B2 JP5972265B2 (ja) | 2016-08-17 |
Family
ID=43033435
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013521142A Active JP5972265B2 (ja) | 2010-07-29 | 2011-07-27 | ジェネリックｐｃｒ |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP2598653B1 (https=) |
| JP (1) | JP5972265B2 (https=) |
| CN (1) | CN103270171B (https=) |
| CA (1) | CA2802550C (https=) |
| ES (1) | ES2644797T3 (https=) |
| WO (1) | WO2012013732A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8609340B2 (en) | 2010-07-29 | 2013-12-17 | Roche Molecular Systems, Inc. | Control nucleic acids for multiple parameters |
| ES2644949T3 (es) * | 2014-08-28 | 2017-12-01 | F. Hoffmann-La Roche Ag | Oligonucleótidos para controlar la amplificación de ácidos nucleicos |
| WO2018045425A1 (en) * | 2016-09-07 | 2018-03-15 | St Vincent's Hospital Sydney Limited | Methods of detecting lentivirus |
| CN108642145A (zh) * | 2018-05-18 | 2018-10-12 | 上海默礼生物医药科技有限公司 | 一种用于核酸实时恒温扩增的试剂、扩增体系及其扩增方法和应用 |
| GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
| MX2021008567A (es) * | 2019-01-17 | 2021-09-21 | Univ Northwestern | Reaccion en cadena de la polimerasa cuantitativa con retrotranscripcion rapida. |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE373444C (de) | 1923-04-12 | Otto Ulrich | Handstaubsauger | |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| ATE138106T1 (de) | 1988-07-20 | 1996-06-15 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| US5035996A (en) | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| CA2035010C (en) | 1990-01-26 | 1996-12-10 | Keith C. Backman | Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
| JPH06502766A (ja) | 1990-11-14 | 1994-03-31 | アクゾ・ノベル・ナムローゼ・フェンノートシャップ | ポリスチレン支持体ベース−サンドイッチハイブリダイゼーションアッセイ法を用いる核酸の非同位体検出およびそれに用いる組成物 |
| US5386024A (en) | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
| US5968729A (en) * | 1994-06-10 | 1999-10-19 | Kosak; Kenneth M. | Use of centrifugation to prepare a retractable seal over reagents in a reaction container |
| ES2253778T3 (es) | 1996-06-04 | 2006-06-01 | University Of Utah Research Foundation | Sistema y metodos de monitorizacion de una amplificacion de adn mediante fluorescencia. |
| JP4540754B2 (ja) | 1996-06-04 | 2010-09-08 | ユニバーシティ オブ ユタ リサーチ ファウンデーション | Ｐｃｒ中のハイブリダイゼーションのモニタリング |
| US5981235A (en) | 1996-07-29 | 1999-11-09 | Promega Corporation | Methods for isolating nucleic acids using alkaline protease |
| US6261770B1 (en) * | 1997-05-13 | 2001-07-17 | Display Systems Biotech Aps | Method to clone mRNAs |
| AU777823B2 (en) * | 1999-01-19 | 2004-11-04 | Somalogic, Inc. | Method and apparatus for the automated generation of nucleic acid ligands |
| WO2001037291A1 (en) | 1999-11-17 | 2001-05-25 | Roche Diagnostics Gmbh | Magnetic glass particles, method for their preparation and uses thereof |
| WO2002012263A1 (en) | 2000-08-03 | 2002-02-14 | Roche Diagnostics Gmbh | Nucleic acid binding compounds containing pyrazolo[3,4-d]pyrimidine analogues of purin-2,6-diamine and their uses |
| ES2305023T3 (es) | 2000-10-31 | 2008-11-01 | F. Hoffmann-La Roche Ag | Metodos para el analisis de componentes no proteinaceos usando una proteasa de una cepa de bacilo. |
| US8962293B2 (en) * | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8895240B2 (en) * | 2008-05-19 | 2014-11-25 | E I Du Pont De Nemours And Company | Method, kit and system for collecting and processing samples for DNA and RNA detection |
| ES2439951T3 (es) * | 2008-06-06 | 2014-01-27 | F. Hoffmann-La Roche Ag | Detección y cuantificación multiplex de ácidos nucleicos microbianos controlada de forma interna |
-
2011
- 2011-07-27 JP JP2013521142A patent/JP5972265B2/ja active Active
- 2011-07-27 CN CN201180046829.XA patent/CN103270171B/zh active Active
- 2011-07-27 WO PCT/EP2011/062957 patent/WO2012013732A1/en not_active Ceased
- 2011-07-27 ES ES11735890.3T patent/ES2644797T3/es active Active
- 2011-07-27 CA CA2802550A patent/CA2802550C/en active Active
- 2011-07-27 EP EP11735890.3A patent/EP2598653B1/en active Active
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