CN103270171B - 通用pcr - Google Patents

通用pcr Download PDF

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Publication number
CN103270171B
CN103270171B CN201180046829.XA CN201180046829A CN103270171B CN 103270171 B CN103270171 B CN 103270171B CN 201180046829 A CN201180046829 A CN 201180046829A CN 103270171 B CN103270171 B CN 103270171B
Authority
CN
China
Prior art keywords
nucleic acid
target nucleic
polymerase
amplification
reaction vessel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201180046829.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN103270171A (zh
Inventor
M.埃克霍夫
N.希齐格
D.齐默尔曼
T.W.迈尔斯
S.G.威尔
E.S.史密斯
H.威尔茨
A.穆斯曼
C.纽豪斯
N.牛顿
J.T.尼米克
N.肖恩布伦纳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN103270171A publication Critical patent/CN103270171A/zh
Application granted granted Critical
Publication of CN103270171B publication Critical patent/CN103270171B/zh
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B01L3/50851—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201180046829.XA 2010-07-29 2011-07-27 通用pcr Active CN103270171B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US36896610P 2010-07-29 2010-07-29
US61/368,966 2010-07-29
EP10175545 2010-09-07
EP10175545.2 2010-09-07
PCT/EP2011/062957 WO2012013732A1 (en) 2010-07-29 2011-07-27 Generic pcr

Publications (2)

Publication Number Publication Date
CN103270171A CN103270171A (zh) 2013-08-28
CN103270171B true CN103270171B (zh) 2014-09-17

Family

ID=43033435

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201180046829.XA Active CN103270171B (zh) 2010-07-29 2011-07-27 通用pcr

Country Status (6)

Country Link
EP (1) EP2598653B1 (https=)
JP (1) JP5972265B2 (https=)
CN (1) CN103270171B (https=)
CA (1) CA2802550C (https=)
ES (1) ES2644797T3 (https=)
WO (1) WO2012013732A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8609340B2 (en) 2010-07-29 2013-12-17 Roche Molecular Systems, Inc. Control nucleic acids for multiple parameters
EP2990491B1 (en) * 2014-08-28 2017-07-12 F. Hoffmann-La Roche AG Oligonucleotides for controlling amplification of nucleic acids
JP7203017B2 (ja) * 2016-09-07 2023-01-12 セイント ヴィンセンツ ホスピタル シドニー リミテッド レンチウイルスを検出する方法
CN108642145A (zh) * 2018-05-18 2018-10-12 上海默礼生物医药科技有限公司 一种用于核酸实时恒温扩增的试剂、扩增体系及其扩增方法和应用
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
US20220064707A1 (en) * 2019-01-17 2022-03-03 Northwestern University Rapid reverse transcription quantitative polymerase chain reaction

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090280539A1 (en) * 2006-10-18 2009-11-12 Roche Molecular Systems, Inc. Dna polymerases and related methods

Family Cites Families (24)

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Publication number Priority date Publication date Assignee Title
DE373444C (de) 1923-04-12 Otto Ulrich Handstaubsauger
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
DE69118930T2 (de) 1990-01-26 1997-01-09 Abbott Lab Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
US5474895A (en) 1990-11-14 1995-12-12 Siska Diagnostics Inc. Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
US5386024A (en) 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
US5968729A (en) * 1994-06-10 1999-10-19 Kosak; Kenneth M. Use of centrifugation to prepare a retractable seal over reagents in a reaction container
JP4281877B2 (ja) 1996-06-04 2009-06-17 ユニバーシティ オブ ユタ リサーチ ファウンデーション 生物学的プロセスを実行し且つモニタリングするためのシステムと方法
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
US5981235A (en) 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
US6261770B1 (en) * 1997-05-13 2001-07-17 Display Systems Biotech Aps Method to clone mRNAs
AU777823B2 (en) * 1999-01-19 2004-11-04 Somalogic, Inc. Method and apparatus for the automated generation of nucleic acid ligands
PT1232502E (pt) 1999-11-17 2006-05-31 Roche Diagnostics Gmbh Particulas de vidro magneticas, metodo para a sua preparacao e suas utilizacoes
WO2002012263A1 (en) 2000-08-03 2002-02-14 Roche Diagnostics Gmbh Nucleic acid binding compounds containing pyrazolo[3,4-d]pyrimidine analogues of purin-2,6-diamine and their uses
ES2305023T3 (es) 2000-10-31 2008-11-01 F. Hoffmann-La Roche Ag Metodos para el analisis de componentes no proteinaceos usando una proteasa de una cepa de bacilo.
US8895240B2 (en) * 2008-05-19 2014-11-25 E I Du Pont De Nemours And Company Method, kit and system for collecting and processing samples for DNA and RNA detection
ES2439951T3 (es) * 2008-06-06 2014-01-27 F. Hoffmann-La Roche Ag Detección y cuantificación multiplex de ácidos nucleicos microbianos controlada de forma interna

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090280539A1 (en) * 2006-10-18 2009-11-12 Roche Molecular Systems, Inc. Dna polymerases and related methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Automated multiplex assay system for simultaneous detection of hepatitis B virus DNA, hepatitis C virus RNA, and human immunodeficiency virus type 1 RNA;Meng Q et al;《Journal of Clinical Microbiology》;20010831;第39卷(第8期);第2937-2945页，参见摘要和材料和方法部分 *

Also Published As

Publication number Publication date
CA2802550A1 (en) 2012-02-02
JP5972265B2 (ja) 2016-08-17
CN103270171A (zh) 2013-08-28
EP2598653B1 (en) 2017-08-23
CA2802550C (en) 2019-01-15
WO2012013732A1 (en) 2012-02-02
EP2598653A1 (en) 2013-06-05
ES2644797T3 (es) 2017-11-30
JP2013542713A (ja) 2013-11-28

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