JP2013512866A - アピコンプレクサFerlin、Ferlin様タンパク質、及び他のC2ドメイン含有タンパク質に基づくマラリアワクチン - Google Patents
アピコンプレクサFerlin、Ferlin様タンパク質、及び他のC2ドメイン含有タンパク質に基づくマラリアワクチン Download PDFInfo
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- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
- A61K2039/6031—Proteins
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26702609P | 2009-12-05 | 2009-12-05 | |
| US61/267,026 | 2009-12-05 | ||
| PCT/EP2010/007399 WO2011066995A1 (en) | 2009-12-05 | 2010-12-06 | Malaria vaccines based on apicomplexan ferlins, ferlin-like proteins and other c2-domain containing proteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013512866A true JP2013512866A (ja) | 2013-04-18 |
| JP2013512866A5 JP2013512866A5 (cg-RX-API-DMAC7.html) | 2017-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2012541362A Pending JP2013512866A (ja) | 2009-12-05 | 2010-12-06 | アピコンプレクサFerlin、Ferlin様タンパク質、及び他のC2ドメイン含有タンパク質に基づくマラリアワクチン |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US8968750B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2507259B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2013512866A (cg-RX-API-DMAC7.html) |
| CN (1) | CN102781958B (cg-RX-API-DMAC7.html) |
| BR (1) | BR112012013581A8 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2783107C (cg-RX-API-DMAC7.html) |
| MX (1) | MX2012006458A (cg-RX-API-DMAC7.html) |
| WO (1) | WO2011066995A1 (cg-RX-API-DMAC7.html) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017210441A (ja) * | 2016-05-26 | 2017-11-30 | 国立大学法人大阪大学 | マラリアワクチン |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012076157A2 (en) | 2010-12-06 | 2012-06-14 | Ruprecht-Karls-Universität Heidelberg | Malaria vaccines based on pre-erythrocytic antigens from p. falciparum |
| ES2847930T3 (es) | 2013-01-07 | 2021-08-04 | Mucosal Vaccine Tech Llc | Vacunas terapéuticas para el tratamiento de las infecciones por el virus del herpes simple de tipo 2 |
| TWI790593B (zh) | 2014-08-19 | 2023-01-21 | 美商默沙東有限責任公司 | 抗tigit抗體 |
| CN108290936B (zh) * | 2015-08-14 | 2022-07-12 | 默沙东公司 | 抗tigit抗体 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09510877A (ja) * | 1994-03-31 | 1997-11-04 | アイシス・イノベーション・リミテッド | マラリアペプチド |
| WO2004037856A2 (en) * | 2002-10-25 | 2004-05-06 | Institut Pasteur | Plasmodium falciparum virulence factor var o |
| WO2004078099A2 (en) * | 2003-03-03 | 2004-09-16 | Pevion Biotech Ltd. | Compositions and methods for the generation of protective immune response against malaria |
| WO2005063804A1 (en) * | 2003-12-30 | 2005-07-14 | Københavns Universitet Panum | Compounds useful in the diagnosis and treatment of malaria |
| WO2009082440A2 (en) * | 2007-12-18 | 2009-07-02 | Vaxinnate Corporation | Compositions of toll-like receptor agonists and malaria antigens and methods of use |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4518528A (en) * | 1983-05-19 | 1985-05-21 | Rasnick David W | α Amino fluoro ketones |
| US5198334A (en) * | 1989-07-21 | 1993-03-30 | Terumo Corporation | Protection of natural killer cell cytolytic activity in peripheral blood mononuclear cells |
| JP2001509813A (ja) * | 1997-01-21 | 2001-07-24 | ニューヨーク ユニヴァーシティ | 抗マラリアワクチン用ユニバーサルt細胞エピトープ |
| US20030077653A1 (en) * | 2000-04-20 | 2003-04-24 | The University Of Georgia Research Foundation, Inc. | Method for the identification of active site protease inactivators |
| US7811575B2 (en) * | 2001-04-10 | 2010-10-12 | Agensys, Inc. | Nucleic acids and corresponding proteins entitled 158P3D2 useful in treatment and detection of cancer |
| GB0210128D0 (en) * | 2002-05-02 | 2002-06-12 | Chiron Spa | Nucleic acids and proteins from streptococcus groups A & B |
| CA2536819A1 (en) * | 2003-08-26 | 2005-03-03 | The Council Of The Queensland Institute Of Medical Research | Immunogenic agent and pharmaceutical composition for use against homologous and heterologous pathogens including plasmodium spp |
| US7951782B2 (en) * | 2005-09-29 | 2011-05-31 | Maruha Nichiro Seafoods, Inc | Composition effective to prevent or treat adult disease |
| GB0601059D0 (en) * | 2006-01-19 | 2006-03-01 | Univ Durham | Target |
| US8343498B2 (en) * | 2008-10-12 | 2013-01-01 | Massachusetts Institute Of Technology | Adjuvant incorporation in immunonanotherapeutics |
| US8277812B2 (en) * | 2008-10-12 | 2012-10-02 | Massachusetts Institute Of Technology | Immunonanotherapeutics that provide IgG humoral response without T-cell antigen |
| US8343497B2 (en) * | 2008-10-12 | 2013-01-01 | The Brigham And Women's Hospital, Inc. | Targeting of antigen presenting cells with immunonanotherapeutics |
| WO2011085070A2 (en) * | 2010-01-06 | 2011-07-14 | Virginia Tech Intellectual Properties, Inc. | Methods and compositions to improve the health of plants, animals and microbes by manipulating protein entry into symbionts and their hosts |
| WO2012076157A2 (en) * | 2010-12-06 | 2012-06-14 | Ruprecht-Karls-Universität Heidelberg | Malaria vaccines based on pre-erythrocytic antigens from p. falciparum |
-
2010
- 2010-12-06 CN CN201080063115.5A patent/CN102781958B/zh not_active Expired - Fee Related
- 2010-12-06 BR BR112012013581A patent/BR112012013581A8/pt not_active Application Discontinuation
- 2010-12-06 MX MX2012006458A patent/MX2012006458A/es unknown
- 2010-12-06 JP JP2012541362A patent/JP2013512866A/ja active Pending
- 2010-12-06 WO PCT/EP2010/007399 patent/WO2011066995A1/en not_active Ceased
- 2010-12-06 US US13/513,059 patent/US8968750B2/en active Active
- 2010-12-06 EP EP10787325.9A patent/EP2507259B1/en not_active Not-in-force
- 2010-12-06 CA CA2783107A patent/CA2783107C/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09510877A (ja) * | 1994-03-31 | 1997-11-04 | アイシス・イノベーション・リミテッド | マラリアペプチド |
| WO2004037856A2 (en) * | 2002-10-25 | 2004-05-06 | Institut Pasteur | Plasmodium falciparum virulence factor var o |
| WO2004078099A2 (en) * | 2003-03-03 | 2004-09-16 | Pevion Biotech Ltd. | Compositions and methods for the generation of protective immune response against malaria |
| WO2005063804A1 (en) * | 2003-12-30 | 2005-07-14 | Københavns Universitet Panum | Compounds useful in the diagnosis and treatment of malaria |
| WO2009082440A2 (en) * | 2007-12-18 | 2009-07-02 | Vaxinnate Corporation | Compositions of toll-like receptor agonists and malaria antigens and methods of use |
Non-Patent Citations (10)
| Title |
|---|
| JPN6015004114; Trends in Parasitology 24, 2, 2007, p.74-84 * |
| JPN6015004117; Infection and Immunity 75, 12, 2007, p.5947-5955 * |
| JPN6015004120; Phil. Trans. R. Soc. Lond. B 357, 2002, p.25-33 * |
| JPN6015004124; Science 307, 2005, p.82-86 * |
| JPN6015004127; Nature 419, 2002, p.498-511 * |
| JPN6015004130; Nature 419, 2002, p.512-519 * |
| JPN6015004136; Journal of the Neurological Sciences 260, 2007, p.114-123 * |
| JPN6015004138; Trends in Cell Biology 14, 4, 2004, p.206-213 * |
| JPN6015053142; NCBI[online], Accession No. XM_001348668, <URL: http://www.ncbi.nlm.nih.gov/nuccore/124809863?sat=13 * |
| JPN7015000319; EMBL/GenBank/DDBJ databases , 200803 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017210441A (ja) * | 2016-05-26 | 2017-11-30 | 国立大学法人大阪大学 | マラリアワクチン |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2507259B1 (en) | 2016-08-24 |
| BR112012013581A8 (pt) | 2017-12-26 |
| CN102781958B (zh) | 2017-07-11 |
| US8968750B2 (en) | 2015-03-03 |
| MX2012006458A (es) | 2012-09-07 |
| CA2783107C (en) | 2018-08-07 |
| WO2011066995A1 (en) | 2011-06-09 |
| BR112012013581A2 (pt) | 2017-01-10 |
| CN102781958A (zh) | 2012-11-14 |
| CA2783107A1 (en) | 2011-06-09 |
| US20120308598A1 (en) | 2012-12-06 |
| EP2507259A1 (en) | 2012-10-10 |
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