JP2013512675A - スクロースからグリセリンおよびグリセリン由来生産物を生産するための組換え細菌 - Google Patents

スクロースからグリセリンおよびグリセリン由来生産物を生産するための組換え細菌 Download PDF

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Publication number
JP2013512675A
JP2013512675A JP2012542212A JP2012542212A JP2013512675A JP 2013512675 A JP2013512675 A JP 2013512675A JP 2012542212 A JP2012542212 A JP 2012542212A JP 2012542212 A JP2012542212 A JP 2012542212A JP 2013512675 A JP2013512675 A JP 2013512675A
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Prior art keywords
seq
sucrose
polypeptide
glycerin
activity
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Ceased
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JP2012542212A
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English (en)
Japanese (ja)
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JP2013512675A5 (https=
Inventor
アンドルー・シー・エリオット
アンソニー・エー・ゲーテンビー
ディーク ティナ・ケイ・ヴァン
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EIDP Inc
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EI Du Pont de Nemours and Co
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Publication of JP2013512675A publication Critical patent/JP2013512675A/ja
Publication of JP2013512675A5 publication Critical patent/JP2013512675A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012542212A 2009-12-04 2010-12-03 スクロースからグリセリンおよびグリセリン由来生産物を生産するための組換え細菌 Ceased JP2013512675A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26660509P 2009-12-04 2009-12-04
US61/266,605 2009-12-04
PCT/US2010/058832 WO2011069033A1 (en) 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Publications (2)

Publication Number Publication Date
JP2013512675A true JP2013512675A (ja) 2013-04-18
JP2013512675A5 JP2013512675A5 (https=) 2013-12-12

Family

ID=43499925

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012542212A Ceased JP2013512675A (ja) 2009-12-04 2010-12-03 スクロースからグリセリンおよびグリセリン由来生産物を生産するための組換え細菌

Country Status (10)

Country Link
US (1) US20110136190A1 (https=)
EP (1) EP2507377A1 (https=)
JP (1) JP2013512675A (https=)
KR (1) KR20120099116A (https=)
CN (1) CN103080326A (https=)
AU (1) AU2010325895B2 (https=)
BR (1) BR112012012719A2 (https=)
CA (1) CA2780757A1 (https=)
SG (1) SG181152A1 (https=)
WO (1) WO2011069033A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8129170B1 (en) * 2010-12-06 2012-03-06 E.I. Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
US8673602B2 (en) * 2011-08-16 2014-03-18 E I Du Pont De Nemours And Company Recombinant bacteria having improved sucrose utilization
US8629243B2 (en) 2011-08-16 2014-01-14 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria
US8686114B2 (en) * 2012-03-05 2014-04-01 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides
US9017961B2 (en) 2012-03-05 2015-04-28 E.I. Du Pont De Nemours And Company Recombinant bacteria comprising novel sucrose transporters

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001346578A (ja) * 2000-04-26 2001-12-18 Ajinomoto Co Inc アミノ酸生産菌及びアミノ酸の製造法
US20040152174A1 (en) * 2002-10-04 2004-08-05 Cervin Marguerite A. Process for the biological production of 1,3-propanediol with high yield
JP2006501851A (ja) * 2002-10-04 2006-01-19 ジェネンコー・インターナショナル・インク 生物資源の生産に対するグルコース輸送変異体
US20060252136A1 (en) * 2005-05-05 2006-11-09 Perry Caimi Utilization of fructose in microbial production strains
WO2008116853A1 (en) * 2007-03-23 2008-10-02 Metabolic Explorer Microorganisms and methods for production of 1,2-propanediol and acetol
WO2010051849A1 (en) * 2008-11-07 2010-05-14 Metabolic Explorer Use of sucrose as substrate for fermentative production of 1,2-propanediol

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
ID21555A (id) * 1996-11-13 1999-06-24 Du Pont Metoda untuk produksi gliserol oleh organisme-organisme yang direkombinasi
JP4327909B2 (ja) * 1996-11-13 2009-09-09 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 組み換え生物による１，３―プロパンジオールの生産方法
WO1999028480A1 (en) 1997-12-02 1999-06-10 E.I. Du Pont De Nemours And Company Method for the production of glycerol by recombinant organisms
BRPI0013315B8 (pt) 1999-08-18 2018-02-27 Du Pont fragmento de ácido nucleico isolado, polipeptídeo, gene quimérico, microorganismo, microorganismo recombinante , e. coli recombinante, linhagem klp23 de e. coli recombinante, linhagem rj8 de e. coli recombinante, vetor pdt29, vetor pkp32 e processos de bioprodução de 1,3-propanodiol
EP2374879A3 (en) 2002-04-22 2012-01-11 E. I. du Pont de Nemours and Company Promoter and plasmid system for genetic engineering

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001346578A (ja) * 2000-04-26 2001-12-18 Ajinomoto Co Inc アミノ酸生産菌及びアミノ酸の製造法
US20040152174A1 (en) * 2002-10-04 2004-08-05 Cervin Marguerite A. Process for the biological production of 1,3-propanediol with high yield
JP2006501851A (ja) * 2002-10-04 2006-01-19 ジェネンコー・インターナショナル・インク 生物資源の生産に対するグルコース輸送変異体
JP2006512907A (ja) * 2002-10-04 2006-04-20 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 高収率での１，３−プロパンジオールの生物生産のための方法
US20060252136A1 (en) * 2005-05-05 2006-11-09 Perry Caimi Utilization of fructose in microbial production strains
WO2008116853A1 (en) * 2007-03-23 2008-10-02 Metabolic Explorer Microorganisms and methods for production of 1,2-propanediol and acetol
WO2010051849A1 (en) * 2008-11-07 2010-05-14 Metabolic Explorer Use of sucrose as substrate for fermentative production of 1,2-propanediol

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J. BACTERIOL. (2002) VOL.184, NO.19, P.5307-5316, JPN6014041103, ISSN: 0002907763 *

Also Published As

Publication number Publication date
BR112012012719A2 (pt) 2015-09-29
AU2010325895A1 (en) 2012-05-31
KR20120099116A (ko) 2012-09-06
SG181152A1 (en) 2012-07-30
CN103080326A (zh) 2013-05-01
US20110136190A1 (en) 2011-06-09
EP2507377A1 (en) 2012-10-10
WO2011069033A1 (en) 2011-06-09
CA2780757A1 (en) 2011-06-09
AU2010325895B2 (en) 2014-11-06

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