JP2013510905A - サイトカイン及びｎｆ−ｋｂに影響を与える組成物及び方法 - Google Patents
サイトカイン及びｎｆ−ｋｂに影響を与える組成物及び方法 Download PDFInfo
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26163909P | 2009-11-16 | 2009-11-16 | |
| US61/261,639 | 2009-11-16 | ||
| PCT/US2010/056862 WO2011060427A2 (en) | 2009-11-16 | 2010-11-16 | Composition and methods for affecting cytokines and nf-kb |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013510905A true JP2013510905A (ja) | 2013-03-28 |
| JP2013510905A5 JP2013510905A5 (https=) | 2014-01-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2012539974A Pending JP2013510905A (ja) | 2009-11-16 | 2010-11-16 | サイトカイン及びｎｆ−ｋｂに影響を与える組成物及び方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20110117122A1 (https=) |
| EP (1) | EP2501390A4 (https=) |
| JP (1) | JP2013510905A (https=) |
| BR (1) | BR112012011678A2 (https=) |
| CA (1) | CA2780144A1 (https=) |
| WO (1) | WO2011060427A2 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US10166270B2 (en) | 2004-04-23 | 2019-01-01 | Zivo Bioscience, Inc. | Composition and method for affecting cytokines and NF-κB |
| CA2827401A1 (en) | 2010-02-22 | 2011-08-25 | Health Enhancement Products, Inc. | Agents and mechanisms for treating hypercholesterolemia |
| US10232028B2 (en) | 2013-06-13 | 2019-03-19 | Zivo Bioscience, Inc. | Compounds and methods for affecting cytokines |
| EP3258948B1 (en) | 2015-02-16 | 2023-12-06 | Zivo Bioscience, Inc. | Anti-inflammatory and immunomodulatory compositions comprising klebsormidium and uses thereof |
| WO2017142906A1 (en) | 2016-02-16 | 2017-08-24 | Zivo Bioscience, Inc. | Nutritional support for animals via administration of an algal derived supplement |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005112987A2 (en) * | 2004-04-23 | 2005-12-01 | Health Enhancement Products, Inc. | Method of preparation and use of fibrinolytic enzymes in the treatment of disease |
| JP2009518312A (ja) * | 2005-12-02 | 2009-05-07 | ヘルス エンハンスメント プロダクツ インク | 疾患を治療するための植物浸出物の組成物および使用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10480A (en) * | 1854-01-31 | Improvement in electro-magnettc engines | ||
| US15587A (en) * | 1856-08-19 | Aepabattts for boeing artesian wells | ||
| US9479A (en) * | 1852-12-21 | Improvement in rakes to grain-harvesters | ||
| JPS6219528A (ja) * | 1985-07-16 | 1987-01-28 | Kurorera Kogyo Kk | 制癌剤 |
| US6461607B1 (en) * | 1998-08-24 | 2002-10-08 | Ganeden Biotech, Inc. | Probiotic, lactic acid-producing bacteria and uses thereof |
| US6673908B1 (en) * | 1999-02-22 | 2004-01-06 | Nuvelo, Inc. | Tumor necrosis factor receptor 2 |
| EP1212093B1 (en) * | 1999-08-26 | 2004-07-07 | Ganeden Biotech, Inc. | Use of emu oil as a carrier for antifungal, antibacterial and antiviral medications |
| AU7551100A (en) * | 1999-09-27 | 2001-04-30 | Algal Ltd. | Vegetarian foodstuff |
| JP2004505925A (ja) * | 2000-08-10 | 2004-02-26 | オーシャン、ニュートリッション、カナダ、リミテッド | 免疫調節特性を示すクロレラ調製物 |
| US20020119164A1 (en) * | 2000-08-18 | 2002-08-29 | Shoji Uchiyama | Use of agaricus blazei murill to prevent or treat skin and other disorders |
| US7125846B2 (en) * | 2001-11-09 | 2006-10-24 | Novo Nordisk Healthcare A/G | Pharmaceutical composition comprising factor VII polypeptides and factor V polypeptides |
| WO2004044161A2 (en) * | 2002-11-06 | 2004-05-27 | Fraunhofer Usa | Expression of foreign sequences in plants using trans-activation system |
| US20130251698A1 (en) * | 2010-10-04 | 2013-09-26 | Health Enhancement Products, Inc. | Composition and method for affecting cytokines and nf-kb |
| US7807622B2 (en) * | 2004-04-23 | 2010-10-05 | Health Enhancement Products, Inc. | Composition and use of phyto-percolate for treatment of disease |
| US8586053B2 (en) * | 2005-09-21 | 2013-11-19 | Health Enhancement Products, Inc. | Composition and use of phyto-percolate for treatment of disease |
-
2010
- 2010-11-16 CA CA2780144A patent/CA2780144A1/en not_active Abandoned
- 2010-11-16 JP JP2012539974A patent/JP2013510905A/ja active Pending
- 2010-11-16 US US12/947,684 patent/US20110117122A1/en not_active Abandoned
- 2010-11-16 EP EP10830908.9A patent/EP2501390A4/en not_active Ceased
- 2010-11-16 BR BR112012011678A patent/BR112012011678A2/pt not_active Application Discontinuation
- 2010-11-16 WO PCT/US2010/056862 patent/WO2011060427A2/en not_active Ceased
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005112987A2 (en) * | 2004-04-23 | 2005-12-01 | Health Enhancement Products, Inc. | Method of preparation and use of fibrinolytic enzymes in the treatment of disease |
| JP2009518312A (ja) * | 2005-12-02 | 2009-05-07 | ヘルス エンハンスメント プロダクツ インク | 疾患を治療するための植物浸出物の組成物および使用 |
Non-Patent Citations (4)
| Title |
|---|
| JPN6014046735; Ｊｕｌｉｕｓ Ｏｂｅｎ， ｅｔ ａｌ．: 'Ｔｈｅ ｅｆｆｅｃｔｓ ｏｆ ＰｒｏＡｌｇａＺｙｍｅ ｎｏｖｅｌ ａｌｇａｅ ｉｎｆｕｓｉｏｎ ｏｎ ｍｅｔａｂｏｌｉｃ ｓｙｎｄｒｏｍｅ ａｎｄ ｍａｒｋｅｒｓ ｏｆ ｃａｒｄｉｏｖａｓｃｕｌａｒ' Ｌｉｐｉｄｓ Ｈｅａｌｔｈ Ｄｉｓ ６（２０）, 2007, １-９ * |
| JPN6014046736; 'Ｗｅｓｔｅｒｎ Ｇｌｏｒｙ Ｈｏｌｅ Ｉｎｃ． Ｅｎｔｅｒｓ Ｄｅｆｉｎｉｔｉｖｅ Ａｇｒｅｅｍｅｎｔ ｗｉｔｈ Ｈｅａｌｔｈ Ｅｎｈａｎｃｅｍｅｎｔ Ｐｒｏｄｕｃｔｓ Ｉｎｃ．' ｏｎｌｉｎｅ , 20031030 * |
| JPN6014046738; 藤田浩: '炎症反応の機構とその制御のための新戦略 炎症のシグナル伝達の制御および遺伝子操作 ＮＦ-kappaＢ' 医学のあゆみ １９０（１０）, 1999, ９１３-６ * |
| JPN6014046740; 岡田英理子ら: '炎症性腸疾患とサイトカイン' 医学のあゆみ 別冊（１０月）, 2004, ２６５-８ * |
Also Published As
| Publication number | Publication date |
|---|---|
| BR112012011678A2 (pt) | 2018-04-17 |
| EP2501390A2 (en) | 2012-09-26 |
| CA2780144A1 (en) | 2011-05-19 |
| WO2011060427A9 (en) | 2011-09-15 |
| US20110117122A1 (en) | 2011-05-19 |
| WO2011060427A2 (en) | 2011-05-19 |
| EP2501390A4 (en) | 2014-07-02 |
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