JP2013504046A - 分離体 - Google Patents
分離体 Download PDFInfo
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- JP2013504046A JP2013504046A JP2012527246A JP2012527246A JP2013504046A JP 2013504046 A JP2013504046 A JP 2013504046A JP 2012527246 A JP2012527246 A JP 2012527246A JP 2012527246 A JP2012527246 A JP 2012527246A JP 2013504046 A JP2013504046 A JP 2013504046A
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- JP
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- separator
- separation
- mobile phase
- dynamically
- separator according
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/282—Porous sorbents
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
- G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/6069—Construction of the column body with compartments or bed substructure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J2220/82—Shaped bodies, e.g. monoliths, plugs, tubes, continuous beds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/92—Construction of the plate
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
【解決手段】3次元クロマトグラフ用の分離体(1)であって、分離体(1)は空間内で望ましくは互いに直交する3方向(X,Y,Z)に延びる。移動相内に各方向(X,Y,Z)で搬送された検体用に各方向(X, Y, Z)において予め決めておくことができる個々の保持機構(Rx, Ry, Rz)を有する。分離体(1)はモノリシックユニットに形成され、当該分離体(1)はさらに、開放または被覆された経路を有するか、またはモノリシック固定相を有するか、または疑似固定相、特にミセルを有するか、またはマイクロマシン構造として形成されるか、またはパックされたベッドを有するか、または自己組織化マイクロ・ナノ構造を有する。
【選択図】図1
Description
る。
X、Y、Zの3つのすべての方向に沿った成分が最終的に明確に分配される。もう一つの(”時間の”)分離の形式は、成分が最後の工程で完全に分離体を
介して駆動されている時に発生するが、Z方向に選択された保持機構によって分離体から異なる時点で表出する。
2 上縁
3 ストリップ
Claims (6)
- 3次元クロマトグラフ用の分離体であって、前記分離体(1)は空間内で望ましくは互いに直交する3方向(X,Y,Z)に延びると共に、前記移動相内に各方向(X,Y,Z)で搬送された検体用に各方向(X, Y, Z)において予め決めておくことができる個々の保持機構(Rx, Ry, Rz)を有する分離体において、
前記分離体はモノリシックユニットに形成され、当該分離体はさらに、
i)開放または被覆された経路を有するか、または
ii)モノリシック固定相を有するか、または
iii)疑似固定相、特にミセルを有するか、または
iv)マイクロマシン構造として形成されるか、または
v)パックされたベッドを有するか、または
vi)自己組織化マイクロ・ナノ構造
を有することを特徴とする分離体。 - 前記保持機構(Rx, Ry, Rz)の少なくとも1つが前記分離体内部のプリセットされた表面特性または多孔性により設定されていることを特徴とする請求項1に記載の分離体。
- 前記保持機構(Rx, Ry, Rz)の少なくとも1つが、細孔径の特性を変更することにより動的に変更することができることを特徴とする請求項1または2に記載の分離体。
- 例えば、疎水性またはシリカC18材料の固有の陽イオン交換容量に対して、表面特性を動的に変更することができることを特徴とする請求項1から3のいずれか1に記載の分離体。
- a)前記移動相に添加される試薬により、または
b)前記分離体の特定区域内の光誘起反応により、または
c)前記分離体内の熱的または電気的に制御可能な特定区域により、または
d)表面電荷を操作して電気浸透流を生成することにより、
表面特性を動的に変更することができることを特徴とする請求項1から4のいずれか1に記載の分離体。 - 表面電荷が動的に生成され、当該表面電荷は前記移動相の組成の選定によって制御可能であることを特徴とする請求項1から5のいずれか1に記載の分離体。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102009040182.2 | 2009-09-07 | ||
DE102009040182A DE102009040182A1 (de) | 2009-09-07 | 2009-09-07 | Trennkörper |
PCT/EP2010/005470 WO2011026651A1 (en) | 2009-09-07 | 2010-09-07 | Separation body for three-dimensional chromatography |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2013504046A true JP2013504046A (ja) | 2013-02-04 |
JP5636426B2 JP5636426B2 (ja) | 2014-12-03 |
Family
ID=43066656
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012527246A Expired - Fee Related JP5636426B2 (ja) | 2009-09-07 | 2010-09-07 | 分離体 |
Country Status (6)
Country | Link |
---|---|
US (1) | US20120171086A1 (ja) |
EP (1) | EP2475985A1 (ja) |
JP (1) | JP5636426B2 (ja) |
CN (1) | CN102498394B (ja) |
DE (1) | DE102009040182A1 (ja) |
WO (1) | WO2011026651A1 (ja) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4469601A (en) * | 1981-03-17 | 1984-09-04 | Varex Corporation | System and apparatus for multi-dimensional real-time chromatography |
JP2005147845A (ja) * | 2003-11-14 | 2005-06-09 | Toppan Printing Co Ltd | 新規な電気泳動分析方法、それを行うための電気泳動分析装置およびそれに使われる光透過性基材 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1523037C3 (de) * | 1964-12-19 | 1973-11-22 | Egon Prof. Dr. 6601 Scheidt Stahl | Gradient Formkörper zur Anwen dung insbesondere in der dreidimen sionalen Chromatographie |
DE1792083A1 (de) * | 1968-07-19 | 1971-04-29 | Merck Anlagen Gmbh | Zweischichtenplatte und ihre Verwendung zur duennschichtchromatographischen Auftrennung von Aminosaeuren |
ATE29594T1 (de) * | 1981-03-17 | 1987-09-15 | Varex Corp | Verfahren und system fuer multidimensionale echtzeit-chromatographie. |
US4469604A (en) * | 1982-05-25 | 1984-09-04 | Phillips Petroleum Company | Sulfonated phenolic material and its use in post primary oil recovery |
NO994873D0 (no) * | 1999-10-06 | 1999-10-06 | Sinvent As | Metode for syntese og analyse av kombinatoriske kjemibiblioteker |
WO2002082051A2 (en) * | 2001-01-17 | 2002-10-17 | Tubbs Kemmons A | An integrated high throughput system for the analysis of biomolecules |
US6685841B2 (en) * | 2001-02-14 | 2004-02-03 | Gabriel P. Lopez | Nanostructured devices for separation and analysis |
WO2005000226A2 (en) * | 2003-06-06 | 2005-01-06 | Diversa Corporation | Mixed bed multi-dimensional chromatography systems and methods of making and using them |
US20070215547A1 (en) * | 2004-02-17 | 2007-09-20 | Waters Investments Limited | Porous Hybrid Monolith Materials With Organic Groups Removed From the Surface |
EP2106279A2 (en) * | 2007-01-08 | 2009-10-07 | Cryobiophysica, Inc. | Stationary phase gradient chromatography |
CN201348625Y (zh) * | 2008-12-26 | 2009-11-18 | 上海通微分析技术有限公司 | 一种多维色谱联用分离装置 |
-
2009
- 2009-09-07 DE DE102009040182A patent/DE102009040182A1/de not_active Ceased
-
2010
- 2010-09-07 CN CN201080039639.0A patent/CN102498394B/zh not_active Expired - Fee Related
- 2010-09-07 US US13/394,502 patent/US20120171086A1/en not_active Abandoned
- 2010-09-07 WO PCT/EP2010/005470 patent/WO2011026651A1/en active Application Filing
- 2010-09-07 EP EP20100760588 patent/EP2475985A1/en not_active Withdrawn
- 2010-09-07 JP JP2012527246A patent/JP5636426B2/ja not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4469601A (en) * | 1981-03-17 | 1984-09-04 | Varex Corporation | System and apparatus for multi-dimensional real-time chromatography |
JP2005147845A (ja) * | 2003-11-14 | 2005-06-09 | Toppan Printing Co Ltd | 新規な電気泳動分析方法、それを行うための電気泳動分析装置およびそれに使われる光透過性基材 |
Non-Patent Citations (1)
Title |
---|
JPN5012016851; GUIOCHON G: 'THEORETICAL INVESTIGATION OF THE POTENTIALITIES OF THE USE OF A MULTIDIMENSIONAL COLUMN (以下備考)' JOURNAL OF CHROMATOGRAPHY VOL. 255, 19830121, PAGES 415-437, ELSEVIER SCIENCE PUBLISHERS B.V, * |
Also Published As
Publication number | Publication date |
---|---|
CN102498394B (zh) | 2014-11-26 |
WO2011026651A1 (en) | 2011-03-10 |
US20120171086A1 (en) | 2012-07-05 |
DE102009040182A1 (de) | 2011-03-24 |
JP5636426B2 (ja) | 2014-12-03 |
CN102498394A (zh) | 2012-06-13 |
EP2475985A1 (en) | 2012-07-18 |
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