JP2013227263A - Cosmetic or food and drink - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide novel fish collagen and/or derivatives thereof improved in coloring and a fish smell in comparison with conventional fish origin collagen and exhibiting excellent moisturizing effect, water retention effect and skin quality improvement effect when taken orally.SOLUTION: Fish collagen obtained from fish selected from Monacanthidae and/or derivatives thereof, and a cosmetic or food and drink prepared by compounding the fish collagen or one or more of the derivatives thereof are provided. The cosmetic or food and drink prepared by compounding the Monacanthidae fish collagen and/or derivatives thereof is improved in coloring and a fish smell and has excellent moisturizing effect, water retention effect and skin quality improvement effect.

Description

  The present invention relates to a cosmetic or a food or drink containing a collagen derived from fish or a derivative thereof.

Conventionally, collagen used for various uses (formulation raw materials for cosmetics, foods, or medical materials) is generally extracted from collagen tissues of mammals such as cattle and pigs. Since infectious diseases such as mad cow disease and foot-and-mouth disease in pigs frequently occur, the use of mammal-derived collagen has tended to be avoided, and in recent years, fish-derived collagen has attracted attention. So far, collagen derived from fish such as shark, salmon, cod, tuna and the like is known as a fish-derived collagen (for example, Patent Documents 1 to 6). Collagen made from a raw material has a problem that it has a fishy odor and is colored and has a low transmittance, and it is difficult to obtain a collagen that is uncolored and has a high purity and has no fishy odor. There is a need for fish-derived collagen with improved problems. Furthermore, conventional fish collagen is insufficient from the viewpoint of its effectiveness when viewed as a blending raw material for cosmetics or foods and drinks, and more effective collagen is required.
JP 2000-050811 A JP 2003-026581 A JP 2003-092997 A JP 2003-171221 A JP 2002-320222 A JP 2007-151453 A

  The present invention has been made in view of the above-mentioned problems found in collagen derived from fish, and its object is to improve coloring and fish odor compared to conventional collagen derived from fish, and An object of the present invention is to provide novel fish-derived collagen and derivatives thereof having high effectiveness, as well as cosmetics and foods and drinks in which the fish-derived collagen and derivatives thereof are blended.

  As a result of intensive studies to solve the above problems, the inventors of the present invention have improved the coloration and fish odor of the collagen derived from the fish belonging to the family Saccharidae as compared with the conventional fish collagen. It has been found that it has a moisturizing action, a water retaining action and a remarkable skin quality improving effect based on the moisturizing action and a water retaining action, and that it can also be taken orally to achieve the skin quality improving effect.

That is, the present invention is a fish collagen and / or derivative thereof obtained from a fish selected from the family Monacanthidae.
In the present invention, the fish collagen and / or derivative thereof is preferably obtained from fish skin and / or fish scales.
Moreover, it is preferable that the fish selected from the smelt family is one species or two or more species belonging to the genus Stephanolepis, the Aluterus, the Aluterus scriptus, and the Thamnaconus. .
In the present invention, the collagen derivative is, for example, atelocollagen, acylated collagen or hydrolyzed collagen.
In the present invention, the acylated collagen is preferably succinylated collagen.
Moreover, this invention is the cosmetics which mix | blended the 1 type (s) or 2 or more types of the fish collagen obtained from the fish selected from the family Saccharidae (Monacanthidae) and / or its derivative (s).
Moreover, this invention is the food / beverage products which mix | blended the 1 type (s) or 2 or more types of the fish collagen obtained from the fish chosen from the smelt family (Monacanthidae) and / or its derivative (s).
In the present specification, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.

  The fish collagen obtained from the fish selected from the smelt family of the present invention has an excellent high moisturizing action and moisture holding action as compared with conventional fish collagen, and is excellent when applied to the skin and hair. The skin quality improvement effect (skin, gloss, texture, dullness, etc.) is exhibited. Furthermore, the collagen of the fish species of the present invention or a derivative thereof exhibits an effect of improving skin quality by ingesting it. In addition, collagen derivatives such as atherates, acylated products, and hydrolysates derived from fish collagen obtained here can also be obtained with excellent quality. It is useful as a blending raw material for foods and foods. Further, the fish collagen or derivative thereof of the present invention has improved coloring and fish odor as compared with conventional fish collagen.

Hereinafter, the present invention will be described in detail.
The fish species used for the preparation of collagen in the present invention may be any fish from the family Saccharidae, for example, the genus Stephanolepis (Stephanolepis)
cirrhifer); Aluterus (Aluterus)
monoceros), Souterhagi (Aluterus scriptus); Thamnaconus
modestus, Thamnaconus modestoides; Cantherhines cantherhines multilineatus, Cantherhines fronticinctus; Rudarius rudarius
ercodes); Paragonacanthus japonicus and Paramonacanthus pusillus, but the present invention is not limited thereto.

  In producing the collagen of the present invention using the above fish species as a raw material, there is no particular limitation on the fish parts used for collagen extraction and collection, and fish skin, fish scales, salmon, tails, bones, meaty parts, etc. should be used. Among them, the use of fish skin and fish scales is preferable from the viewpoint of collagen yield, workability, and the like. The collagen production method of the present invention will be described in detail below by taking the case of using fish skin as an example.

  First, using any one or more of the above-described fish species, the head, tail, coral, meaty part and bone part are removed to obtain a raw fish skin. Furthermore, about fish skin, in order to extract collagen efficiently, this is first shredded into a size of about 5 to 30 mm square. Or it is good also as a strip shape about 5-20 mm in width.

  Next, this fish skin shredded product is washed with water prior to the extraction of collagen to remove unnecessary materials. In this case, in order to sufficiently remove unnecessary materials, it is desirable to perform washing with water under a bath ratio of 2 to 100, preferably 5 to 50 hours, for about 1 to 48 hours, preferably about 12 to 36 hours. Further, when washing is performed with stirring or homogenizing using a mixer or the like, washing can be performed more effectively.

  Further, alkali treatment may be carried out, and the alkali treatment may be performed by immersing the skin in an aqueous solution of sodium hydroxide, potassium hydroxide, calcium hydroxide or the like and stirring. For example, in the case of sodium hydroxide, it is preferable to use an aqueous solution of 0.01 to 0.5 mol / L, preferably 0.05 to 0.2 mol / L. The temperature during the alkali treatment is desirably 10 ° C. or lower. The immersion is desirably performed for about 1 to 48 hours, preferably about 12 to 36 hours, and is more effective when repeated several times.

  After the washing treatment, the fish skin is drained and subjected to a collagen extraction step using an acid aqueous solution. As the acid, organic acids such as acetic acid, lactic acid, succinic acid, and citric acid are preferably used, and citric acid is most preferable. The concentration of the aqueous acid solution varies depending on the acid used, but generally a concentration such that the pH of the aqueous solution is in the range of 3 to 4 is used. For example, in the case of acetic acid, the concentration is 0.1 to 0.5 M. The concentration is preferred. Collagen extraction is 10 to 100 times by weight, preferably 20 to 50 times the amount of acid aqueous solution with respect to the wet weight of the fish skin shredded product, preferably 2 to 15 ° C. for 1 to 72 hours, preferably Is carried out by stirring for 12 to 48 hours. It should be noted that it is desirable to add a proteolytic enzyme to the extraction bath during acid extraction because it is possible to increase the yield of collagen and remove telopeptide (atelolysis).

After the extraction is completed, the fish residue of the extraction residue is removed from the extract by filtration or centrifugation, and then the salted-out treatment in which salt is added to the clarified extract obtained here for precipitation, and an alkali is added. A point precipitation treatment and an organic solvent treatment in which an organic solvent is added are applied to precipitate collagen from the extract.
The isoelectric precipitation is performed by adding an aqueous alkaline solution to the clarified extract so that the final pH is 7 to 10, preferably 8 to 9, and stirring. As the alkaline substance, sodium hydroxide, potassium hydroxide and the like are preferably used. A protein mainly composed of collagen is precipitated by this isoelectric point precipitation treatment, and is collected by an appropriate means such as filtration or centrifugation.

  After the isoelectric point precipitation treatment, purification treatment is performed. The purification treatment is performed by thoroughly washing the collagen obtained by the isoelectric point precipitation treatment.

  Purified fish collagen obtained by the above purification treatment is substantially uncolored and has a purity that is almost all of the body surface pigments removed during the production process of the fish skin shredded product washing process and acid extraction process. It can be used as a raw material for cosmetics and foods and drinks as it is, but if desired, it can be further treated with the organic solvent treatment described below to obtain higher quality collagen. it can.

  In the organic solvent treatment, the purified fish collagen is suspended in an organic solvent in an amount of 0.5 to 5 times, preferably 1 to 2 times the dry weight of the dry weight, and 0.1 to 24 hours, preferably 0.1. After stirring for 5 to 1 hour, the collagen is collected using an appropriate means such as filtration or centrifugation. As the organic solvent, for example, ethanol, isopropanol, acetone or the like is used. This organic solvent treatment removes trace amounts of lipid components, odorous components, and the like remaining in the purified fish collagen, but the removal of these components becomes more effective by repeating the above operation.

  If the collagen is to be used in the form of a solution, the solid purified fish collagen with or without the above-mentioned organic solvent treatment is treated with an acid solution such as citric acid or citric acid-sodium citrate. What is necessary is just to redissolve in a neutral salt solution so that it may become a desired density | concentration, and it may clarify by performing filtration as needed.

  The production of the fish collagen of the present invention using fish skin as a raw material is carried out as described above, but also when using fish scales, tails, salmon, meaty parts, bones, etc. as raw materials, the above-mentioned fish skin is used. By applying the same treatment as in, fish collagen with excellent quality can be obtained.

  The fish collagen of the present invention obtained as described above is useful as it is as a raw material for cosmetics or food and drink, but is further subjected to a succination treatment or the like to chemically modified collagen ( For example, succinylated collagen etc.) or hydrolyzed with acid, alkali, enzyme etc. to obtain hydrolysates (eg hydrolyzed collagen, tripeptide etc.), and raw materials for cosmetics and foods It can also be blended as In addition, when such a collagen derivative is used, a derivative having a higher quality than that using conventional fish collagen as a starting material can be obtained corresponding to the high quality of the fish collagen of the present invention used as a raw material. Is possible.

  Examples of fish collagen derivatives by chemical modification include acylated derivatives such as succinylated collagen, phthalated collagen, maleylated collagen, myristyl-succinylated collagen, and the like.

  The acylated derivative of fish collagen can be produced by reacting a desired acylating agent with purified fish collagen according to a conventionally known method for preparing acylated collagen.

  As an example, a method for producing succinylated fish collagen is as follows. First, an alkali such as sodium hydroxide or potassium hydroxide is added to an aqueous solution of purified fish collagen to adjust the pH to 9 to 12, and the collagen is insolubilized to obtain a collagen dispersion. As an acylating agent, 1 to 10 times (weight ratio) of succinic anhydride as an acylating agent is directly added or dissolved in acetone, ethanol, methanol or the like, and gradually added while stirring at a cold temperature. During this time, the pH of the reaction solution is adjusted with an alkali such as sodium hydroxide or potassium hydroxide so that it does not fall below 9. When the insolubilized collagen is dissolved, the reaction is stopped, and an acid is added to the reaction solution to adjust the pH to 4-5. At this time, since the succinylated collagen is insolubilized and precipitated, it is recovered by filtration or centrifugation, and washed with water and dried if necessary to obtain succinylated fish collagen. This succinylated fish collagen dissolves well in neutral aqueous solutions of pH 5-8.

  The preparation of the hydrolyzate of fish collagen of the present invention can be carried out by any method similar to the conventional method for preparing a collagen hydrolyzate. For example, 0.0005 to 0.01 times (weight ratio) of proteolytic enzyme of collagen is added to an aqueous solution of purified fish collagen, and the mixture is kept at the optimum pH and temperature of the enzyme for 1 to 4 hours for hydrolysis. After carrying out the decomposition treatment, the enzyme is inactivated, followed by filtration and the like to remove impurities, and further adjusting the pH as desired, whereby a fish collagen hydrolyzate solution can be obtained. If necessary, the fish collagen hydrolyzate may be separated from the solution as a solid according to a conventional method.

  The fish collagen and derivatives thereof of the present invention obtained as described above (hereinafter sometimes collectively referred to as fish collagen) are not only excellent in safety to the human body because they are derived from fish. In terms of quality, it has excellent properties such as high moisture retention and moisture retention, which are not conventional, and is useful as a blending raw material for cosmetics, foods and drinks, and the like.

  Cosmetics comprising the fish collagens of the present invention include, for example, basic cosmetics such as emulsions, creams, lotions, essences, packs, face wash, makeup such as lipsticks, foundations, liquid foundations, makeup press powders, etc. Examples include cosmetics, hair shampoos, hair rinses, hair treatments, conditioners, hair dyes, hair cosmetics such as hairdressing agents, and bath preparations, but are not limited to these.

  The amount of fish collagen in the cosmetic of the present invention may be the same as in the case of conventional collagen-containing cosmetics. For example, in the case of basic cosmetics, 0.003 to 0.30% by weight, preferably in the range of 0.03 to 0.25% by weight. In the case of makeup cosmetics, the solid content of collagen is generally 0.003 to 0.15% by weight. In the case of hair cosmetics, the solid content of collagen is generally 0.003 to 0.25% by weight, preferably 0.03 to 0.3%. In the range of 15% by weight

  When preparing a cosmetic containing the fish collagens of the present invention, the components thereof are usually components used in cosmetics, such as oily components, surfactants, moisturizers, thickeners, antiseptic / disinfectants, powders. Body components, ultraviolet absorbers, antioxidants, pigments, fragrances, physiologically active components, and the like can be used.

  Here, as the oily component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, etc. Fats from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; hydrocarbons such as liquid paraffin, petrolatum, paraffin wax, squalane; myristic acid, palmitic acid Fatty acids such as stearic acid, oleic acid and isostearic acid; higher alcohols such as lauryl alcohol, cetanol and stearyl alcohol; isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, higher fatty acid octyldodecyl (stear Synthetic esters such as phosphate octyldodecyl) and synthetic triglycerides, and the like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
Moreover, lecithin and its derivatives, lactic acid bacteria fermented rice, etc. can also be mix | blended as an emulsifier thru | or an emulsification adjuvant.

  Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, lactic acid bacteria fermented rice, hyaluronic acid and Examples thereof include lactic acid, urea, higher fatty acid octyldodecyl, various amino acids, and derivatives thereof.

  Examples of thickeners include brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae-derived components; polysaccharides such as pectin, locust bean gum and aloe polysaccharides; gums such as xanthan gum, tragacanth gum and guar gum; Cellulose derivatives such as methylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and derivatives thereof, polyglutamic acid and derivatives thereof, and the like.

  Examples of the antiseptic disinfectant include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkco chloride Examples thereof include nium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazolidene urea), and 1,2-pentanediol.

  Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, silk powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate and the like.

  Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and derivatives thereof.

  Examples of physiologically active ingredients include whitening ingredients such as t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone derivatives, ellagic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4-methoxy Salicylic acid potassium salt, magnolignan (5,5′-dipropyl-biphenyl-2,2′-diol), 4-HPB (rhodenol, 4- (4-hydroxyphenyl) -4-butanol)), AMP (adenosine monophos) Fate, adenosine monophosphate), placenta extract, nicotinic acid and its derivatives, Sakuhakuhi extract, yukinoshita extract, rice bran extract, rice bran extract hydrolyzate, lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals ( Wheat, beans, cereals), white coconut extract, white coconut hydrolyzed extract Murasakixikib extract, lotus seed fermented product, ginseng extract, pearl barley fermented product, royal jelly fermented product, sake lees fermented product, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract , Chamomile extract (trade name: Chamomira ET), seaweed extract such as kombu, seaweed extract such as sea cucumber, linoleic acid and its derivatives or processed products (eg liposomal linoleic acid), 2,5- Dihydroxybenzoic acid derivatives, etc. are also used as skin aging prevention and skin beautifying components. Collagens derived from animals or fish (fishes other than cormorants) and derivatives thereof, elastin and derivatives thereof, nicotinic acid and derivatives thereof, glycyrrhizic acid and derivatives thereof (Dipotassium salts, etc.), t-cycloamino acid derivatives, vitamin A and its derivatives Herbal extracts such as vitamin E and derivatives thereof, allantoin, α-hydroxy acids, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, pearl barley extract, chamomile extract, carrot extract, aloe extract, There are rice extract hydrolyzate, rice bran extract hydrolyzate, rice fermentation extract, honey comb extract, anaaaosa extract, seaweed extract such as sea bream, sohakuhi extract, and Zizyphus joazeiro extract.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α) -D-glucopyranosyl-L-ascorbine Acid) ascorbic acid sugar derivatives, acylated 6-positions of these ascorbic acid sugar derivatives (acyl groups are hexanoyl, octanoyl, decanoyl, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetra Examples of L-ascorbic acid tetrafatty acid esters such as lauric acid ester include resorcinol derivatives such as 4-n-butylresorcinol and 4-isoamylresorcinol, and examples of 2,5-dihydroxybenzoic acid derivatives include 2, 5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid and the like are nicotinic acid derivatives such as nicotinic acid amide and benzyl nicotinate as vitamin E derivatives. For example Glutamic E nicotinate, vitamin E linoleate and the like, as the α- hydroxy acids such as lactic acid, citric acid, α- hydroxy octanoic acid.

  Moreover, the fish collagen of this invention can be mix | blended with various drinks and foodstuffs. For example, beverages such as beauty drinks, energy drinks, sports drinks, near water, vitamin drinks, mineral drinks, alcoholic drinks; foods such as various soups (including powdered soups), dairy products, jelly, candy, tablet confectionery, gum ; Although it can mix | blend with a tablet, a liquid, a granular form, or a jelly-form health food and drink etc., this invention is not limited to this, It can mix | blend with the food-drinks etc. which can be taken orally.

  Next, the present invention will be described more specifically with reference to examples, formulation examples (cosmetic examples), and test examples, but the present invention is not limited thereto. In the following, all parts are by weight, and all% are by weight.

Example 1. Preparation of kawahagi collagen aqueous solution Stephanolepis
cirrhifer) skin 30 g was thawed and chopped to 10-20 mm square. Add 300 mL of 0.3% aqueous sodium hydroxide to the shredded product, homogenize with a juicer mixer, and discard the liquid phase three times. Wash the fish skin shredded product with 0.3% aqueous sodium hydroxide. did. Next, 900 mL of purified water was added, homogenized with a juicer mixer, and the operation of discarding the liquid phase part was repeated three times to sufficiently wash the fish skin shredded product. Next, the washed fish skin was sufficiently drained, 900 mL of 0.5 M acetic acid aqueous solution containing 0.03 g of pepsin was added thereto, and the mixture was stirred at 4 to 10 ° C. for 24 hours to extract collagen. After filtering this extract to remove the fish skin of the extraction residue, sodium chloride was added to the resulting clarified extract to a final concentration of 1M with stirring, and stirring was continued for 1 hour to obtain atelocollagen collagen. Was precipitated. The precipitated collagen was collected by centrifugation, suspended in 900 mL of 20 mM disodium hydrogen phosphate solution, adjusted to pH 8 using 10% aqueous sodium hydroxide solution, stirred for 24 hours, and centrifuged again. Collagen was collected. Next, the operation of suspending the recovered collagen in 900 mL of purified water, washing with stirring and centrifuging is repeated three times, and then the obtained purified collagen is dissolved in 0.02 wt% citric acid aqueous solution to obtain a solid content. 220 g of a substantially colorless and odorless collagen aqueous solution at a concentration of 0.5% by weight was obtained. The above operation was performed under conditions such that the room temperature and the liquid temperature were both 15 ° C. or less.

Example 2 Preparation of Usubahagi Collagen Aqueous Solution By using the same procedure as in Example 1 except that Usuhahagi (Aluterus monoceros) is used instead of Stephanolepis cirrhifer in Example 1, it is substantially colorless and odorless at a solid content concentration of 0.5% by weight. 200 g of an aqueous collagen solution was obtained.

Example 3 Preparation of an aqueous solution of quail hull collagen Stephanolepis of Example 1
205 g of a substantially colorless and odorless collagen aqueous solution with a solid content concentration of 0.5% by weight was obtained in the same manner as in Example 1 except that Thamnaconus modestus was used instead of cirrhifer.

Example 4 Preparation of Kawahagi Collagen Powder In Example 1, instead of dissolving purified collagen in 0.02 wt% citric acid aqueous solution to make collagen aqueous solution, purified collagen was freeze-dried and pulverized, as in Example 1. Thus, 1.1 g of white collagen powder was obtained.

Example 5 FIG. Preparation of Aqueous Collagen Aqueous Solution To 200 g of a collagen aqueous solution obtained in the same manner as in Example 1, 800 g of a 0.02 wt% aqueous citric acid solution is added. This aqueous solution was adjusted to pH 9.5 with a 1 mol / L potassium hydroxide aqueous solution. To this, 5 g of succinic anhydride dissolved in 20 mL of ethanol was gradually added and stirred at a low temperature (about 4 ° C.) for 12 hours. During this period, the pH of the reaction solution was adjusted with a 1 mol / L potassium hydroxide aqueous solution so that it did not fall below 9. After completion of the reaction, 1 mol / L hydrochloric acid was added to the reaction solution to adjust the pH to 4.5, and the insoluble succinylated collagen was collected by centrifugation. This was washed with 0.01 mol / L hydrochloric acid, dried under reduced pressure, dissolved in a citrate buffer solution (pH 6.3), and 150 g of a succinylated collagen aqueous solution having a solid content concentration of 0.5% by weight and substantially colorless and odorless. Got. The above operation was performed under conditions such that the room temperature and the liquid temperature were both 15 ° C. or lower.

Example 6 Preparation of Kawahagi Collagen Aqueous Solution Collagen powder obtained in the same manner as in Example 1 was dispersed in purified water to prepare 100 g of a collagen dispersion having a solid content of 1% by weight. Hydrolyzed proteolytic enzyme was added to this liquid. 0.01% by weight was added, and the enzyme was hydrolyzed by stirring for 1 hour while keeping the temperature at 40 to 50 ° C. After completion of hydrolysis, the temperature was raised to 80 ° C., kept warm for 30 minutes to inactivate the enzyme, and filtered to obtain 90 g of a substantially colorless and odorless hydrolyzed hemocollagen solution at a solid content concentration of 1%.

Example 7 Preparation of Aqueous Collagen Aqueous Solution A scale of 5 ml of kawahagi air-dried at room temperature was stirred in 120 mL of cooled acetone for 1 hour, and then scales were collected by suction filtration. Similarly, the acetone treatment is repeated twice. The scales collected by suction filtration were dried under reduced pressure. 200 mL of 0.5 mol / L hydrochloric acid was added to the dried scales, and the mixture was stirred for 24 hours while cooling to decalcify. After the decalcification, scales were collected by centrifugation. 1000 mL of purified water and 0.05 g of pepsin were added to the collected scales, and collagen was extracted by stirring at 4 to 10 ° C. for 48 hours. After filtering this extract to remove the scales of the extraction residue, sodium chloride was added to the resulting clarified extract to a final concentration of 1M with stirring, and stirring was continued for 1 hour to precipitate collagen. It was. The precipitated collagen was collected by centrifugation, suspended in 900 mL of 20 mM disodium hydrogen phosphate solution, adjusted to pH 8 using 10% aqueous sodium hydroxide solution, stirred for 24 hours, and centrifuged again to obtain collagen. Was recovered. Next, the operation of suspending the recovered collagen in 900 mL of purified water, washing with stirring and centrifuging is repeated three times, and then the obtained purified collagen is dissolved in 0.02 wt% citric acid aqueous solution to obtain a solid content. 130 g of a substantially colorless and odorless collagen aqueous solution at a concentration of 0.5% by weight was obtained. The above operation was performed under conditions such that the room temperature and the liquid temperature were both 15 ° C. or less.

Comparative Example 1 Preparation of Salmon Collagen Aqueous Solution 150 g of a substantially colorless collagen aqueous solution having a solid content concentration of 0.3% by weight was obtained in the same manner as in Example 1 except that salmon (Oncorhynchus keta) skin was used instead of the peeled skin. .

Comparative Example 2 Preparation of Tuna Collagen Aqueous Solution 160 g of collagen aqueous solution having a solid content concentration of 0.3% by weight was obtained in the same manner as in Example 1 except that tuna (Thunnus thynnus: blue tuna) skin was used in place of the peeled eel. This solution had a light gray color, and it was difficult to make it colorless even by decolorization treatment. The tuna collagen aqueous solution had a fishy odor.

Comparative Example 3 Preparation of a bovine nosita collagen aqueous solution 120 g of a substantially colorless collagen aqueous solution having a solid content concentration of 0.3% by weight was obtained in the same manner as in Example 1 except that the skin of the sea buckthorn (Paraplagusia japonica) was used. Obtained.

Comparative Example 4 Preparation of aqueous solution of shark collagen Using shark (Prionace glauca) skin instead of eelfish skin, an extraction operation similar to that of Example 1 was carried out to obtain 300 g of a substantially colorless collagen aqueous solution having a solid content concentration of 0.3% by weight. Obtained.

Although the example of the cosmetics and food / beverage products which mix | blended the Kawahagi collagen of this invention is shown below, the detailed report of the cosmetics and foodstuff which mix | blended this invention is not restricted to this. In addition, the compounding quantity of the following each component shows the weight% with respect to the whole cosmetics or foodstuff.
Formulation Example 1 Cream [component A]
Squalane 5.0
Olive oil 4.0
Paraffin 5.0
Cetanol 2.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Sorbitan monostearate 2.0
[B component]
1,3-butylene glycol 10.0
Methylparaben 0.1
Ethylparaben 0.05
Purified water Amount of 100 parts [component C]
Fragrance 0.1
Kawahagi collagen aqueous solution of Example 1 20.0
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 40 degrees C or less, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and obtained the homogeneous cream.

Formulation Example 2 Emulsion [Component A]
Squalane 10.0
Olive oil 4.0
Polyoxyethylene (20) glyceryl monostearate 1.0
Glyceryl monostearate 1.0
[B component]
1,3-butylene glycol 5.0
Methylparaben 0.1
Ethylparaben 0.05
Purified water Amount of 100 parts [component C]
Fragrance 0.1
Kawahagi collagen aqueous solution of Example 1 15.0
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 40 degrees C or less, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and obtained the homogeneous emulsion.

Formulation Example 3 Lotion [Ingredient]
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Citric acid 0.3
Sodium citrate 0.6
Kawahagi collagen aqueous solution of Example 5 20.0
Purified water A total amount of 100 parts All the raw materials were combined, mixed by stirring and dissolved to obtain a transparent lotion.

Formulation Example 4 Essence [ingredient]
Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Kawahagi collagen aqueous solution of Example 1 30.0
Citric acid 0.3
Sodium citrate 0.6
Purified water in an amount of 100 parts Hyaluronic acid was dissolved in purified water, and then the remaining raw materials were sequentially added and dissolved by stirring to obtain a transparent essence.

Formulation Example 5 Emulsion A homogenous emulsion was obtained in the same manner as in Formulation Example 2 except that, in Component C of Formulation Example 2, the aqueous solution of Usubahagi collagen of Example 2 was used instead of the aqueous solution of Kawahagi collagen of Example 1.

Formulation Example 6 Emulsion A homogeneous emulsion was obtained in the same manner as in Formulation Example 2 except that the aqueous solution of eelfish collagen of Example 3 was used instead of the aqueous solution of Kawahagi collagen of Example 1 in Component C of Formulation Example 2.

Formulation Example 7 Emulsion A homogenous emulsion was obtained in the same manner as in Formulation Example 2, except that, in Component C of Formulation Example 2, the Kawahagi collagen aqueous solution of Example 5 was used instead of the Kawahagi collagen aqueous solution of Example 1.

Formulation Example 8 Emulsion A homogeneous emulsion was obtained in the same manner as in Formulation Example 2, except that, in Component C of Formulation Example 2, instead of the Kawahagi collagen aqueous solution of Example 1, the Kawahagi collagen aqueous solution of Example 6 was used.

Formulation Example 9 Emulsion A homogenous emulsion was obtained in the same manner as in Formulation Example 2, except that, in Component C of Formulation Example 2, the Kawahagi collagen aqueous solution of Example 7 was used instead of the Kawahagi collagen aqueous solution of Example 1.

Formulation Example 10 Emulsion [Component A]
Squalane 10.0
Olive oil 4.0
Polyoxyethylene (20) glyceryl monostearate 1.0
Glyceryl monostearate 1.0
Ethyl paraaminobenzoate 1.0
[B component]
1,3-butylene glycol 5.0
Methylparaben 0.1
Ethylparaben 0.05
Kojic acid 1.0
Purified water Amount of 100 parts [component C]
Fragrance 0.1
Kawahagi collagen aqueous solution of Example 1 15.0
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 40 degrees C or less, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and obtained the homogeneous emulsion.

Formulation Example 11 Emulsion [Component A]
Squalane 10.0
Olive oil 4.0
Polyoxyethylene (20) glyceryl monostearate 1.0
Glyceryl monostearate 1.0
[B component]
1,3-butylene glycol 5.0
Methylparaben 0.1
Ethylparaben 0.05
L-ascorbic acid-2-glucoside 2.0
Purified water Amount of 100 parts [component C]
Fragrance 0.1
Kawahagi collagen aqueous solution of Example 1 15.0
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 40 degrees C or less, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and obtained the homogeneous emulsion.

Formulation Example 12. Emulsion In the same manner as in Formulation Example 11, except that 3.0 parts of L-ascorbic acid-2-phosphate magnesium is used instead of 2.0 parts of L-ascorbic acid-2-glucoside in the B component of Formulation Example 11. A homogeneous emulsion was obtained.

Formulation Example 13 Emulsion In the same manner as Formulation Example 11, except that 3.0 parts of L-ascorbic acid-2-phosphate is used instead of 2.0 parts of L-ascorbic acid-2-glucoside in the B component of Formulation Example 11. A homogeneous emulsion was obtained.

Formulation Example 13 Emulsion A homogeneous emulsion was obtained in the same manner as in Formulation Example 11 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside in the component B of Formulation Example 11.

Formulation Example 14. Emulsion In the B component of Formulation Example 11, instead of 2.0 parts of L-ascorbic acid-2-glucoside, a rice bran extract hydrolyzate (trade name “Gracenow * Snow * HP”, manufactured by Technoble Co., Ltd., solid content A homogeneous emulsion was obtained in the same manner as in Formulation Example 11 except that 5.0 parts (concentration 3.5%) were used.

Formulation Example 15. Emulsion White component extract (manufactured by Technoble Co., Ltd., trade name “Sinablanca-WH”, solid content concentration 1.0%) in place of 2.0 parts of L-ascorbic acid-2-glucoside in component B of formulation example 11 5 A homogeneous emulsion was obtained in the same manner as in Formulation Example 11 except that 0.0 part was used.

Formulation Example 16. Emulsion A homogeneous emulsion in the same manner as in Formulation Example 11 except that 1.0 part of γ-amino-β-hydroxybutyric acid is used instead of 2.0 parts of L-ascorbic acid-2-glucoside in the B component of Formulation Example 11. Got.

Formulation Example 17. Emulsion [Component A]
Squalane 10.0
Olive oil 4.0
Polyoxyethylene (20) glyceryl monostearate 1.0
Glyceryl monostearate 1.0
[B component]
1,3-butylene glycol 5.0
Methylparaben 0.1
Ethylparaben 0.05
Rice extract hydrolyzate 5.0
(Technoble Co., Ltd., trade name “Orizen Noble”, solid content 1.5%)
Purified water Amount of 100 parts [component C]
Fragrance 0.1
Kawahagi collagen aqueous solution of Example 5 15.0
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 40 degrees C or less, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and obtained the homogeneous emulsion.

Formulation Example 18. Hair treatment [A component]
Stearyltrimethylammonium chloride 5.0
Glyceryl monostearate 1.0
Cetanol 3.0
Octyldodecanol 2.0
[B component]
1,3-butylene glycol 5.0
Methylparaben 0.1
Purified water Amount of 100 parts [component C]
Fragrance 0.2
Kawahagi collagen aqueous solution of Example 1 20.0
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 40 degrees C or less, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less.

Formulation Example 19. Liquid foundation [component A]
Cetanol 0.5
Glyceryl monostearate 2.0
Lanolin 2.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) sorbitan monostearate 2.0
[B component]
Xanthan gum 0.2
1,3-butylene glycol 5.0
Methylparaben 0.1
Ethylparaben 0.05
Purified water Amount of 100 parts [component C]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount [component D]
Fragrance 0.1
Kawahagi collagen aqueous solution of Example 1 15.0
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 40 degrees C or less, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring further.

Formulation Example 20. Cream foundation [component A]
Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) sorbitan monostearate 2.0
[B component]
Sorbitol 3.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Ethylparaben 0.05
Purified water Amount of 100 parts [component C]
Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount [component D]
Fragrance 0.3
Kawahagi collagen aqueous solution of Example 10.0 10.0
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 40 degrees C or less, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring further.

Formulation Example 21. Cream rinse [A component]
Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.0
Stearyl alcohol 1.0
[B component]
1,3-butylene glycol 5.0
Methylparaben 0.1
Purified water Amount of 100 parts [component C]
Usubahagi collagen aqueous solution of Example 2 20.0
After each component A and component B are heated to 80 ° C. and uniformly dissolved, component B is added to component A, and stirring is continued until it is cooled to 40 ° C. or lower. It cooled to 30 degrees C or less, stirring.

Formulation Example 22. Cream shampoo [component A]
N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
1,3-butylene glycol 2.0
Purified water Amount [component B] that makes the total amount 100 parts
Kawahagi collagen aqueous solution of Example 1 20.0
The component A was heated to 80 ° C. and dissolved uniformly. After cooling to 40 ° C. or lower, the component B was added, and the mixture was further cooled to 30 ° C. or lower with stirring.

Formulation Example 23. Body shampoo [A component]
N-lauroylmethylalanine sodium 20.0
Coconut oil fatty acid potassium solution (40%) 20.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
1,3-butylene glycol 5.0
Purified water Amount [component B] that makes the total amount 100 parts
Horseshoe Collagen Collagen aqueous solution of Example 20.0
The component A was heated to 80 ° C. and dissolved uniformly. After cooling to 40 ° C. or lower, the component B was added, and the mixture was further cooled to 30 ° C. or lower with stirring.

Formulation Example 24. Beverage Kawahagi collagen aqueous solution of Example 1 8.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Antioxidant (Vitamin C) 0.01
Amount of purified water totaling 100 parts

Formulation Example 25. Beverage Kawahagi Collagen Aqueous Solution of Example 5 8.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Antioxidant (Vitamin C) 0.01
Amount of purified water totaling 100 parts

Formulation Example 26. Beverage Kawahagi collagen aqueous solution of Example 6 8.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Antioxidant (Vitamin C) 0.01
Amount of purified water totaling 100 parts

Formulation Example 27. Beverage Kawahagi collagen aqueous solution of Example 7 8.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Antioxidant (Vitamin C) 0.01
Amount of purified water totaling 100 parts

Formulation Example 28. Kawahagi Collagen Aqueous Solution of Tablet Example 1 20
Vitamin C 20
Fatty acid ester 10
Calcium lactate 20
Lactose 30
After the above-mentioned parts by weight of each component were mixed, they were pressure-molded to obtain tablets.

Comparative formulation example 1
In Formulation Example 4, an essence was obtained in the same manner as Formulation Example 4, except that the salmon collagen aqueous solution of Comparative Example 1 was used instead of the Kawagi collagen aqueous solution of Example 1.

Comparative formulation example 2
In Formulation Example 4, an essence was obtained in the same manner as Formulation Example 4 except that the tuna collagen aqueous solution of Comparative Example 2 was used in place of the Kawahagi collagen aqueous solution of Example 1.

Comparative formulation example 3
In Formulation Example 4, an essence was obtained in the same manner as in Formulation Example 4, except that the aqueous solution of bovine calf collagen of Comparative Example 3 was used instead of the aqueous solution of Kawahagi collagen of Example 1.

Comparative Formulation Example 4
In Formulation Example 4, an essence was obtained in the same manner as in Formulation Example 4 except that the shark collagen aqueous solution of Comparative Example 4 was used instead of the Kawagi collagen aqueous solution of Example 1.

Comparative Formulation Example 5
In Formulation Example 24, a beverage was obtained in the same manner as Formulation Example 24 except that the salmon collagen aqueous solution of Comparative Example 1 was blended in place of the Kawahagi collagen aqueous solution of Example 1.

Comparative Formulation Example 6
In Formulation Example 24, a beverage was obtained in the same manner as Formulation Example 24, except that the tuna collagen aqueous solution of Comparative Example 2 was blended in place of the Kawahagi collagen aqueous solution of Example 1.

Comparative Formulation Example 7
In Formulation Example 24, a beverage was obtained in the same manner as Formulation Example 24 except that the aqueous bovine collagen solution of Comparative Example 3 was blended in place of the Kawahagi collagen aqueous solution of Example 1.

Comparative Formulation Example 8
In Formulation Example 24, a beverage was obtained in the same manner as Formulation Example 24, except that the shark collagen aqueous solution of Comparative Example 4 was blended in place of the Kawahagi collagen aqueous solution of Example 1.

Test Example 1 Moisture retention test of collagen Using the various collagens produced in the examples and comparative examples as test substances, a moisture retention test by male and female panelists was performed.
[Test method]
Six test sites of 1 cm square were set on the forearm of five subjects, and the initial moisture content of each site was measured using an impedance meter (SKIN SURFACE HYGROMETER
SKICON-200 IBS) was measured three times and the average value was obtained.
Next, 20 μL of each test substance was applied to each test site, and after 10, 20, and 30 minutes from application, the skin moisture content was measured three times, and the average value of the increase in skin moisture was determined.
Increase in skin moisture (ΔμS) =
Skin moisture at each measurement time (μS)-Initial skin moisture (μS)

The test results are shown in Table 1.
[Table 1]

As a result, the collagen obtained from the fish skin or fish scale extract of kawahagi, usubahagi, horse mackerel, and fish scales shown in Examples 1 to 3 and 5 to 7 is a collagen obtained from conventionally known salmon, tuna, bovine nose and shark fish skin. As compared with, it was recognized that it had a much better moisturizing effect.

Test Example 2 Water Retention Test of Collagen Aqueous Solution A water retention test was performed using various collagen aqueous solutions produced in Examples and Comparative Examples as test substances.
[Test method]
A 15 mm × 15 mm 1C filter paper was placed on a 20 mm × 20 mm plastic piece, and the initial weight was measured. 50 μL of each sample was dropped on a filter paper, measured for weight, allowed to stand at room temperature (room temperature 20 ° C., humidity 40%), and weighed 60 minutes later.
Evaporation amount (g) = initial weight (g) −measured weight after 60 minutes (g)

The test results are shown in Table 2.
[Table 2]

As a result, the collagen obtained from the fish skin or fish scale extract of kawahagi, usubahagi, horse mackerel, and fish scales shown in Examples 1 to 3 and 5 to 7 is a collagen obtained from conventionally known salmon, tuna, bovine nose and shark fish skin. Compared to the above, it was confirmed that the water retention power was much better.

Test Example 3 Monitor test (essence)
Essences containing various collagen aqueous solutions manufactured in Examples and Comparative Examples were manufactured, and a moisturizing effect and a skin improvement effect test by a monitor test were performed.
[sample]
(1) Invention Sample: Essence of Formulation Example 4 (2) Comparative Sample 1: Essence of Comparative Formulation Example 1 (3) Comparative Sample 2: Essence of Comparative Formulation Example 2 (4) Comparative Sample 3: Comparative Formulation Example 3 Essence (5) Comparative Sample 4: Essence of Comparative Formulation Example 4

[Test method]
40 randomly selected women aged 20 to 55 years were taken as subjects and divided into 4 groups (A, B, C, D) of 10 people per group. Separately, it was applied twice a day (morning and evening) for one month, and a comparative test was performed.
The use samples and application sites of each group are as follows.
Group A: left face of the present invention sample / comparative sample 1 right face B group: comparison sample 2 left face / right face sample of the present invention C group: left face sample of the present invention / comparative sample 3 right face D group: comparison sample 4 left face / Invention sample right face [Evaluation method]
Select the values that are considered to be applicable from the following five evaluation languages based on the self-judgment of the five items of “moist feeling”, “smoothness”, “texture”, “harness” and “dullness” of the left and right facial skin. It was.
5: Very good (remarkably improved).
4: Good (remarkably improved).
3: Slightly good (slightly improved).
2: Neither good nor bad (no change).
1: Bad (the condition has deteriorated).
In addition, after the monitor test was completed, for each sample applied to the subjects in each group, it was self-determining which sample had the highest moisturizing and skin quality improving effect (advantage determination in comprehensive evaluation). It was selected by evaluation.

Table 3 shows the evaluation results of the five items, and Table 4 shows the results of the comprehensive evaluation.
[Table 3]

[Table 4]

From the results in Tables 3 and 4 above, collagen obtained from the fish skin of kingfisher has superior skin improvement effect (moist feeling, smoothness, smoothness) than collagen obtained from fish skin such as salmon, tuna, bovine nose and shark. It was found to have a texture, firmness and dullness.

Test Example 4 Monitor test (drink)
Beverages containing various aqueous collagen solutions produced in Examples and Comparative Examples were produced, and a moisturizing effect and a skin improvement effect test by a monitor test were conducted.
[sample]
(1) Invention Sample: Beverage of Formulation Example 24 (2) Comparison Sample 5: Beverage of Comparison Formulation Example 5 (3) Comparison Sample 6: Beverage of Comparison Formulation Example 6 (4) Comparison Sample 7: Comparison Formulation Example 7 Beverage (5) Comparative Sample 8: Beverage of Comparative Formulation Example 8
[Test method]
Randomly extracted 50-year-old women from 20 to 63 years old were divided into 5 groups of 10 people per group, and each of the beverages of Prescription Example 24 and Comparative Prescription Examples 5-8 was 50 mL (as collagen) 4 g) was orally administered continuously for 8 weeks, and changes in skin condition before and after the administration were investigated. Based on self-judgment, “Numerous feeling”, “Smoothness”, “Texture”, “Hari”, and “Dullness” were selected from the following five levels of evaluation language, and used as evaluation points.
5: Very good (remarkably improved).
4: Good (substantially improved).
3: Slightly good (slightly improved).
2: Neither good nor bad (no change).
1: Bad (the condition has deteriorated).

The results are shown in Table 5.
[Table 5]

As shown in Table 5, the beverage of the sample of the present invention (Formulation Example 23) blended with Kawahagi collagen of Example 1 is a comparative formulation of the collagens of Comparative Examples 1 to 4 (salmon, tuna, bovine nocita, shark-derived collagen). Compared to the drinks of Comparative Samples 5 to 8 formulated based on Examples 5 to 8, it is clearly shown that the oral intake has excellent skin improvement effects (moist feeling, smoothness, texture, firmness and dullness). became.

As described above, the collagen or derivative thereof of the fish selected from the saccharidae of the present invention is colorless and has no fishy odor, and further has excellent moisturizing and water retention by application to the skin or oral intake. In addition, since it has a skin improving effect, it is extremely useful as a blending raw material for cosmetics or foods and drinks.

Claims (7)

Fish collagen and / or derivatives thereof obtained from fish selected from the family Monacanthidae. The fish collagen and / or derivative thereof according to claim 1, wherein the fish collagen and / or derivative thereof are obtained from fish skin and / or fish scales. The fish selected from the family Surgeonfish is one or more fish belonging to the genus Stephanolepis, Aluterus, Aluterus scriptus, and Thamnaconus Item 3. The fish collagen and / or derivative thereof according to item 1 or 2. The fish collagen and / or derivative thereof according to any one of claims 1 to 3, wherein the collagen derivative is atelocollagen, acylated collagen or hydrolyzed collagen. The fish collagen and / or derivative thereof according to claim 4, wherein the collagen derivative is succinylated collagen. Cosmetics which mix | blended 1 type (s) or 2 or more types chosen from the fish collagen of Claims 1 thru | or 5, and / or its derivative (s). Food / beverage products which mix | blended 1 type (s) or 2 or more types selected from the fish collagen of Claim 1 thru | or 5, and / or its derivative (s).