JP2013181031A - Testosterone secretion promotor, antifatigue agent, method for producing the same and use thereof - Google Patents

Abstract

PROBLEM TO BE SOLVED: To develop a testosterone secretion promotor and an antifatigue agent effective for increasing the testosterone level in a body and promoting prevention, amelioration and recovery of fatigue, a composition capable of effectively utilizing the agents in industrial use, and a method for increasing the testosterone level and preventing, ameliorating and recovering fatigue.SOLUTION: There are provided a testosterone secretion promotor, an antifatigue agent and a composition for peroral administration effective for preventing and/or ameliorating sarcopenia, osteopenia, male climacteric disorder, obesity, depression, ED, alopecia, diabetes, coronary arteriopathy, etc., and improving analgesic action, nutritional fortification, muscle building, sexual function, stamina, concentration power, and performance in sports, etc., and comprising black vinegar mash, a combination of black vinegar mash and phosphatidylserine, cereal vinegar and its mash obtained by using soybean, barley and/or brown rice as a raw material and fermenting the material, and a combination of the materials with phosphatidylserine, and a method for orally administering the agents.

Description

  The present invention relates to a testosterone secretion promoter containing a specific fermented product as an active ingredient, an anti-fatigue agent containing the agent, a method for producing the same, and a composition obtained by blending the agent.

  Living organisms usually use saccharides taken from outside the body, saccharides stored in the body or fat, and produce energy in the mitochondria of cells via glycolysis, TCA circuit and electron transfer pathway. When both mind and body are healthy and resting, aerobic energy production is performed and sufficient energy is supplied, but anaerobic energy production increases when prolonged activity or intense exercise is performed, Fatigue-related substances such as lactic acid and active oxygen accumulate in the body.

  Fatigue is a condition in which fatigue-related substances accumulate in the body and the desire for rest has increased. According to an epidemiological survey conducted in 1999 by the Ministry of Health and Welfare (current Ministry of Health, Labor and Welfare) About 60% of the working population (47.2 million people), about half of them are shown to suffer from chronic fatigue that lasts more than half a year (non-patent literature) 1).

  If the body continues to be tired, stiff shoulders, low back pain, headache, coldness, malaise, drowsiness, poor concentration, loss of appetite, sleep disturbance, malaise, etc. Decrease the daily life. In addition to the above, fatigue during exercise causes deterioration of exercise performance and injury, and preventing fatigue is an important issue, and the daily requirement for nutrients such as vitamins, minerals and carbohydrates is increased. It is recommended to eat and eat a nutritionally balanced diet.

  Testosterone is a steroid hormone belonging to androgen and is a kind of male hormone, and its action to increase muscle mass is generally known. In addition, the relation with fatigue is also known (Patent Documents 1 and 2), and in order to promote prevention / reduction and / or recovery of fatigue, it is desirable to increase the testosterone level in the body.

  For this reason, intake of functional coffee (Patent Document 2), processed onion (Patent Document 3), plant of the genus Turnin (Patent Document 4) and the like has been proposed from the viewpoint of increasing the testosterone level in the body.

  However, in order to express the physiological effects of these materials in vivo, there are problems such as the need to ingest a large amount and the possibility of side effects, which are sufficiently satisfactory in practical terms. Almost never found. Accordingly, there has been a demand for the development of effective materials and compositions that increase testosterone levels in the body and prevent, reduce, and / or recover from fatigue.

  By the way, the following is known about black vinegar moromi. Black vinegar moromi is a precipitate produced through fermentation and aging treatment in the production process of black vinegar, and is a solid obtained by squeezing and filtering the precipitate. Black vinegar mash includes protein, dietary fiber, saturated fatty acids such as palmitic acid and stearic acid, unsaturated fatty acids such as oleic acid and linoleic acid, ethyl esters of these fatty acids, phosphorus, potassium, sodium, calcium, etc. It contains minerals and has been studied using animal models to show various physiological activities against symptoms and diseases such as high cholesterol, diabetes, allergies, cancer, etc. There are no reports or proposals on the anti-fatigue effect.

  In addition, phosphatidylserine is a kind of glycerophospholipid and is contained in a large amount in the cell membrane. So far, phosphatidylserine has been studied in humans for its physiological effects on memory, stress, attention deficit hyperactivity disorder, etc., and is known to be useful for the recovery and improvement of brain and nerve functions. (Non-Patent Documents 2 and 3).

Special table 2007-507503 gazette JP 2009-106253 A Japanese Patent Laid-Open No. 9-169661 JP 2007-84559 A

Teruo Kitani, “Study on Fatigue Survey and Fatigue Recovery Methods for Health Promotion”, 1999 Ministry of Health and Welfare Research Results Report, Health Sciences Research Grants Health Sciences Research Project G. Palmieri et al., Clinical Trials Journal, Vol. 24, No. 1, No. 73-83, 1987 Crook T. Neurology, 41, 644-649, 1991

  In view of the current situation, the present invention has developed a testosterone secretion promoter that increases the testosterone level in the body, an anti-fatigue agent that prevents, reduces, and / or recovers fatigue, a composition containing the agent, and testosterone It was an object to provide a method for increasing the value and a method for preventing / reducing and / or recovering fatigue.

  In order to achieve the above-mentioned problems, the present inventors have conducted extensive studies on the testosterone secretion promoting action and anti-fatigue action of various materials, and as a result, the black vinegar-related material and the combination of the material and the specific material are testosterone. Has been found to have the effect of significantly promoting the secretion of lysine and preventing / reducing fatigue and / or promoting recovery, and in the industrial fields such as food and drink, feed, pet food, quasi drugs, pharmaceuticals, etc. Obtaining knowledge that can be used effectively, the present invention has been completed.

  That is, the first feature of the present invention resides in a testosterone secretion promoter comprising (a) black vinegar moromi as an active ingredient. A second feature is a testosterone secretion promoter comprising (b) a combination of black vinegar moromi and phosphatidylserine as an active ingredient.

  The third feature is that, as an active ingredient, (c) cereal vinegar obtained through fermentation and ripening processes using soybean and brown rice and / or barley as raw materials, or (d) soybean and brown rice and / or barley A testosterone secretion promoter characterized by containing moromi of cereal vinegar obtained through a fermentation process and an aging process.

  A fourth feature is that, as an active ingredient, (e) a combination of cereal vinegar and phosphatidylserine obtained through fermentation and ripening processes using soybean and brown rice and / or barley as raw materials, or (f) soybean and A testosterone secretion promoter comprising brown rice and / or barley as a raw material and a combination of moromi of cereal vinegar obtained through a fermentation process and a ripening process and phosphatidylserine. A fifth feature is that black soybean is used as the soybean. Here, the grain vinegar is preferably liquid and / or powder.

  The sixth feature of the present invention resides in a method in which cereal vinegar as an active ingredient of the testosterone secretion promoter is produced by subjecting soybean, brown rice and / or barley to fermentation at the same time.

  A seventh feature is an anti-fatigue agent comprising any one of the above testosterone secretion promoters. The eighth feature is an oral composition comprising the testosterone secretion promoter or anti-fatigue agent. The oral composition is preferably in the form of a food / beverage product, quasi-drug, pharmaceutical product, feed or pet food.

  The testosterone secretion promoter of the present invention includes (a) black vinegar mash, (b) a combination of black vinegar mash and phosphatidylserine, and (c) soybean and brown rice and / or barley as fermented and ripened as active ingredients. Cereal vinegar obtained, (d) mash of the cereal vinegar, (e) combined use of the cereal vinegar and phosphatidylserine of (c), (f) combined use of mash of vinegar and phosphatidylserine of (d) It contains one or more of the substances, is excellent in stability such as quality, and has the effect of increasing the testosterone level in the body based on the active ingredient by taking or administering it orally. Therefore, the testosterone secretion promoter of the present invention can be effectively used as an anti-fatigue agent for preventing / reducing and / or recovering fatigue of a living body, and also includes sarcopenia, osteopenia, male menopause, obesity, depression, Prevent and / or improve ED, hair loss, diabetes, coronary artery disease, etc., and use it in applications such as analgesia, nourishing tonic, muscle building, and improving sexual function, endurance, concentration, performance in sports, etc. It is expected. For this reason, the testosterone secretion promoter and anti-fatigue agent are used in the form of the above-described agents as they are or in various conventional products, particularly in the fields of foods and drinks, quasi drugs, pharmaceuticals, feeds, pet foods and the like. It can be used effectively.

  The present invention is described in detail below. First, one of the aspects of the testosterone secretion promoter of the present invention comprises the above (a): black vinegar mash as an active ingredient.

  The black vinegar moromi used in the testosterone secretion promoter is a solid obtained as a precipitate of brewed vinegar colored brown or black brown through a fermentation process and an aging process in the production process of black vinegar. The production method of black vinegar moromi according to the present invention can employ a common production method of black vinegar, and is not particularly limited.

  In the production process of black vinegar, the black vinegar moromi obtained as a precipitate may be in the form after separating the supernatant black vinegar, and after squeezing the moromi, freeze drying, vacuum drying, infrared drying, etc. It may be dried by the method, pulverized and powdered. As for the black vinegar moromi according to the present invention, it is easy to use a commercially available product.

  As a result of studying a material capable of exerting an even stronger testosterone secretion promoting action and anti-fatigue action when used in combination with black vinegar moromi, the present inventors have found that phosphatidylserine is extremely effective. That is, another embodiment of the testosterone secretion enhancer of the present invention comprises the combination of (b): black vinegar moromi and phosphatidylserine as active ingredients.

  The phosphatidylserine used in the testosterone secretagogue of this embodiment is soy, rapeseed, corn, sunflower, safflower, sesame, evening primrose, borage, flax, cottonseed, palm, olive, palm, etc., plant seeds, egg yolk, seafood, It can be produced by extracting from tissues of animals such as cows, pigs and chickens. Furthermore, this extract is subjected to purification treatment such as silica gel fractionation and solvent fractionation using adsorbents such as silica gel, its octadecyl silylate, magnesium silicate, ion exchange resin, activated alumina, activated carbon, etc. Phosphatidylserine can be prepared. In addition, phosphatidylserine-rich phospholipids can also be produced by using phospholipase D using lecithin produced from the above raw materials as a raw material, and performing a phosphatidyl group transfer reaction. The production method of phosphatidylserine according to the present invention is not particularly limited, and it is easy to use a commercially available phospholipid. In this case, the phosphatidylserine content is desirably 20% by mass or more. Below this, the desired effect of the present invention may be reduced or may not be exhibited.

  The combination ratio of black vinegar moromi and phosphatidylserine used in the testosterone secretion promoter of the present invention can be appropriately changed depending on the purpose and use of the agent, production cost, etc., but black vinegar moromi / phosphatidylserine (mass) Ratio) is generally 99.9 / 0.1 to 10/90, more preferably 99/1 to 30/70.

  Another aspect of the testosterone secretion promoter of the present invention is the above (c): cereal vinegar obtained through fermentation and ripening processes using soybeans and brown rice and / or barley as raw materials, or (d): It contains moromi as an active ingredient.

  According to the vinegar quality labeling standard (Ministry of Agriculture, Forestry and Fisheries Notification No. 1507, revised on October 16, 2008), the grain vinegar in the present invention uses “one or two or more kinds of cereals as raw materials among brewed vinegars” In cereal vinegar defined as “the total amount used is 40 g or more per liter of brewed vinegar” (in the case of agricultural products other than cereals and fruits and honey not using honey) Or although barley is used as a raw material, it is not specifically limited.

  The brown rice used in the present invention is rice other than rice that has been whitened by removing all rice husks and rice straw layers. Specifically, it means brown rice or rice that has been subjected to scouring treatment so as not to completely remove the rice bran portion. To do. The soybean to be used is not particularly limited, but black soybean is more preferable. Moreover, barley used as a raw material in the present invention means barley that has been refined so as not to completely remove the barley or the wheat straw portion of the barley as in the case of the above rice.

  Grain vinegar is usually produced through three stages of saccharification, alcoholic fermentation and acetic acid fermentation by adding straw and water to one or more kinds of cereals, and the cereal vinegar according to the present invention is fermented in the production process. It is a brewed vinegar obtained through a process and an aging process. The production method may be a general static fermentation method or a continuous fermentation method, and is not particularly limited.

  The stationary fermentation method is a traditional Japanese vinegar production method. In the stationary fermentation method, saccharification, alcohol fermentation, and acetic acid fermentation proceed simultaneously, and aging is performed for a long time to produce vinegar. On the other hand, the continuous fermentation method is a method in which air is forcibly fed and stirred vigorously, thereby dramatically increasing the growth of acetic acid bacteria and producing vinegar in a short period of time. In the present invention, moromi produced by any fermentation method can be used.

  The cereal vinegar used in the testosterone secretion promoter of the present invention is a cereal vinegar stock solution, concentrate or dilution thereof produced by the above-mentioned method, and has an acidity of about 0.1% by mass to about 15% by mass. To target. Its form is liquid (cereal vinegar stock), liquid cereal vinegar is solidified or powdered by drying under reduced pressure, freeze drying, spray drying, etc., or powdered by conventional methods with the coexistence of dextrin and lipids Processed products (cereal vinegar powder), for example, those obtained by diluting the above cereal vinegar stock solution with water, for example, about 1.5 times to about 20 times, more preferably about 5 times to about 10 times can be used. It is. When the dilution ratio exceeds about 20 times, the desired effect of the present invention is lowered. Furthermore, in accordance with the spirit of the present invention, components (organic acids, sugars, peptides and proteins, amino acids, vitamins and minerals, polyphenols, phospholipids, glycosides, etc.) contained in cereal vinegar are separated, It may be appropriately concentrated or purified by known means such as fractionation or column chromatography. In addition, the grain vinegar stock solution in the present invention includes both those containing moromi and those removed by filtration or the like.

  A further aspect of the testosterone secretion promoter of the present invention is the above-mentioned (e): a combination of cereal vinegar and phosphatidylserine obtained through fermentation and ripening processes using soybean and brown rice and / or barley as raw materials, or (f ): A combination of moromi of cereal vinegar and phosphatidylserine obtained through soybean fermentation and brown rice and / or barley as a raw material through fermentation and aging processes as active ingredients.

  Next, the anti-fatigue agent of this invention is demonstrated. This anti-fatigue agent contains the above-mentioned testosterone secretion promoter and is an example of an application in which the agent is effectively used. Therefore, the anti-fatigue agent of the present invention comprises (a): black vinegar mash, (b): black vinegar mash and phosphatidylserine combination, (c): fermented soybean and brown rice and / or barley. Cereal vinegar produced by aging treatment, (d): mash of the cereal vinegar, (e): combined use of cereal vinegar and phosphatidylserine of (c), (f): cereal vinegar of (d) Any one or two or more of moromi and phosphatidylserine are used as active ingredients. And as said testosterone secretion promoter, Preferably it contains about 30 mass%-100 mass%, More preferably, it contains about 50 mass% or more, and addition of the well-known activator, stabilizer, diluent, etc. which are mentioned later The product can be molded into a solid, powder, gel, or liquid form by appropriately using the product. The anti-fatigue agent of the present invention can be used in a method of ingesting or administering orally for the purpose of preventing, reducing and / or recovering fatigue of a living body, enhancing endurance and muscle mass, and the like.

  The testosterone secretion promoter and anti-fatigue agent described above have at least one of the above (a) to (f) as active ingredients, which are used as they are, that is, in the form of powder, solid, paste, or gel consisting of the active ingredients. Alternatively, it is in a liquid form and can be used as a product such as a food / beverage product, a pharmaceutical product, a quasi-drug, a feed or a pet food.

  The agent of the present invention can also be used as an oral composition by appropriately combining known additives used in the products for which it can be used, and containing them in a conventional manner. Here, as long as it is not contrary to the gist of the present invention, known additives can use various raw materials and ingredients, and those usually used for oral ingestion, food and drink, pharmaceuticals, quasi drugs, etc. are preferable, for example, Excipients, binders, wetting agents, fluidizers, antioxidants, preservatives, stabilizers, diluents, solubilizers, tonicity agents, pH adjusters, acidulants, sweeteners, colorants, flavorings, etc. In addition to known ingredients (arginine, echinacoside, etc.) that have the effect of promoting the secretion of testosterone, known ingredients (anticitric acid, anserine, carnosine, etc.) that have anti-fatigue action, or materials containing them May be. In addition to foods and drinks, pharmaceuticals, quasi-drugs, feeds, pet foods, the above agents, especially testosterone secretion promoters, are used as a part of ingredients for various oral compositions and products in other industrial fields. You can also. For example, prevention and / or improvement of sarcopenia, osteopenia, male menopause, obesity, depression, ED, hair loss, diabetes, coronary artery disease, etc., analgesia, nutrition tonic, muscle building, and sexual function, endurance, concentration and sports It is expected to be a product for improving performance and other factors.

  When the agent is used in combination with known additives to form an oral composition, it can be an oral preparation such as a powder, granule, tablet, capsule, or liquid. The content of the active ingredient in the composition for oral administration is difficult to define uniformly depending on the type and content of the raw materials used together, but is generally about 0.1% by mass to 100% by mass, more preferably about 10% by mass. To about 100% by weight. If it is less than about 0.1% by mass, the desired effect of the present invention is not recognized. A suitable intake or dosage of the oral composition of the present invention is based on the active ingredient contained in the agent, and is about 10 mg to about 100,000 mg, preferably about 100 mg per day for a human adult (body weight 50 kg). To about 10,000 mg, more desirably about 300 mg to about 5,000 mg.

  The oral composition of the present invention can be used as products such as foods and drinks, pharmaceuticals, quasi drugs, and feeds. Examples of practical products are described below, but the present invention is not limited to these exemplifications.

  Specific examples of food and drink include beverages such as vegetable juice, fruit juice drinks, soft drinks, tea, instant noodles, soup, jelly, pudding, yogurt, cake premix products, confectionery, sprinkles, miso, soy sauce, sauce, dressing , Mayonnaise, vegetable cream, seasonings such as grilled meat sauce and noodle soup, noodles, udon, soba noodles, spaghetti, ham and sausage and other processed meat and fish products, hamburger, croquette, boiled, jam, milk, cream, butter, In addition to various processed foods such as powders such as spreads and cheese, solid or liquid dairy products, margarine, bread, cakes, cookies, chocolate, candy, gummi, gum, etc., powders, granules, pills, Tablet, soft capsule, hard capsule, paste or liquid dietary supplement, food for specified health use, functional food Mention may be made of health food products, the concentrated liquid diet and dysphagia for food diet and the like.

  In order to manufacture these foods and drinks, the above-mentioned agent and known raw materials may be used, or a part of the known raw materials may be replaced with the above-mentioned agent and manufactured by a conventional method. For example, the agent may be added as necessary, such as glucose, glucose, dextrin, lactose, starch or processed product thereof, excipients such as cellulose powder, vitamins, minerals, fats and oils of animals and plants and seafood, proteins (animals and plants and Along with edible materials such as yeast-derived proteins, hydrolysates thereof, carbohydrates, pigments, fragrances, antioxidants, surfactants, other edible additives, powders and extracts containing various nutritional functional ingredients Processed into powders, granules, pellets, tablets, etc. by mixing, processed into the form of general processed foods in the above examples by conventional methods, mixed powders and liquids such as gelatin, sodium alginate, carboxymethylcellulose, etc. It is suitable to use as a dietary supplement or health food by coating with a coating agent and forming into a capsule or processing into a beverage (drink) form. In particular, tablets, capsules and drinks are desirable. In addition, the content and intake of the testosterone secretion promoter and anti-fatigue agent of the present invention contained in these foods and drinks are almost the same as in the case of the aforementioned oral composition.

  Drugs and quasi-drugs using the above agents are processed into tablets by appropriately adding known pharmaceutically acceptable excipients and additives as long as they do not contradict the spirit of the present invention. , Capsules, granules, powders, liquids and the like. It is administered orally and applied to prevent, reduce and / or recover from fatigue, strengthen muscles, improve endurance and concentration, reduce or treat menopause. In addition, the content and intake of the agent contained in these pharmaceuticals and quasi drugs are based on the above-mentioned oral composition.

  In addition, in order to apply the agent to livestock feed and pet food, as in the case of the food and drink, it is blended in various known feeds and drinking water, or in the form of tablets and granules together with known raw materials and additives. It can be processed into a capsule form. The content and intake of the agent in these feeds are almost the same as in the case of the aforementioned oral composition. The composition containing the agent can be used, for example, for improving the fur and gloss of pets, enhancing the endurance and muscle mass of racehorses.

  Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited thereto. In each example, all percentages, parts and ratios are based on mass unless otherwise indicated. The raw brown rice used was from Miyazaki Prefecture and Kagoshima Prefecture, the barley brown wheat from Kagoshima Prefecture, and black soybeans from Tamba, Hyogo Prefecture.

Production Example 1
3.7 kg of yellow koji, 11.6 kg of steamed brown rice and 32 L of water were added to koji koji (hereinafter the same) used for producing black vinegar by a known static fermentation method, and fermentation and aging were carried out for 8 months. Later, squeezed to obtain moromi. The moromi was dried with infrared rays and pulverized to produce black vinegar moromi (sample 1).

Production Example 2
Black vinegar moromi obtained by the same treatment as in Production Example 1 and phosphatidylserine (manufactured by Cargill, USA, trade name: Lipamine (registered trademark) PS90P, the same applies hereinafter) at a ratio of 2: 3 are uniformly mixed to obtain black. A mixture of vinegar moromi and phosphatidylserine (sample 2) was prepared.

Production Example 3
A cereal vinegar (sample 3) containing moromi was fermented and aged for 8 months by adding 3.7 kg of yellow koji, 7.6 kg of steamed brown rice, 4 kg of steamed black soybeans and 32 L of water. Moreover, it was squeezed to produce cereal vinegar (sample 4) and cereal vinegar mash.

Production Example 4
Α-cyclodextrin (manufactured by Wacker Chemie, Germany, trade name: CAVAMAX (registered trademark) W6, the same applies hereinafter) was added to the cereal vinegar (sample 4) described in Production Example 3, lyophilized, ground, and α- Grain vinegar powder (sample 5) containing 50% cyclodextrin was produced.

Production Example 5
Grain vinegar mash as described in Production Example 3 was pulverized and powdered after far-infrared drying to obtain cereal vinegar mash flour (sample 6). Grain vinegar (sample 4) was concentrated under reduced pressure, 10 kg of α-cyclodextrin was added and spray-dried to produce a powder containing 80% α-cyclodextrin. Next, the cereal vinegar mash powder was mixed to produce mash cereal vinegar powder (sample 7) containing α-cyclodextrin 50%.

Production Example 6
50% α-cyclodextrin-containing cereal vinegar powder (sample 7) obtained by the same treatment as in Production Example 5 and phosphatidylserine are uniformly mixed at a ratio of 2: 3, and α-cyclodextrin 50% A mixture of corn vinegar powder containing mash and phosphatidylserine (sample 8) was prepared.

Production Example 7
Grain vinegar mash powder (sample 6) obtained in the same manner as in Production Example 5 and phosphatidylserine were uniformly mixed at a ratio of 2: 3, and a mixture of cereal vinegar mash powder and phosphatidylserine (sample 9) was prepared. Prepared.

Comparative production example 1
After adding 3.7 kg of yellow koji, 7.6 kg of steamed brown rice and 32 L of water to the koji rice cake, fermented for 8 months, black vinegar from which the mash was removed even after pressing was produced.

Comparative production example 2
3.8 kg of black soybeans was soaked in black vinegar obtained by the same treatment as in Comparative Production Example 1 and allowed to stand for 20 days in an indoor dark place to produce cereal vinegar (Comparative Sample 1).

Comparative production example 3
Grain vinegar obtained by processing in the same manner as in Comparative Production Example 3 was pulverized in the same manner as in Production Example 4 to prepare unfermented black soybean extract-containing cereal vinegar powder (Comparative Sample 2).

Test Example 1 (Testosterone secretion promotion test and anti-fatigue test)
Testosterone secretion promoting action and anti-fatigue action were examined by the following methods. That is, a 6-week-old ddy male mouse (Sankyo Lab Service Co., Ltd.) was preliminarily bred for 1 week, 4 groups per group, and a control group administered with distilled water only, phosphatidylserine (US, manufactured by Cargill, product) Name: Lipamine (registered trademark) PS90P) administration group, the present invention sample (samples 1-9) administration group, and comparative sample (black vinegar and comparative samples 1 and 2) administration group. Each sample was orally administered every day, and all the mice were subjected to swimming exercise three times a week and reared for 4 weeks. At the second week of breeding, attach a 2g weight to the mouse's tail, swim in the aquarium, and wait until it becomes tired and the swimming movement in which the top of the mouse sinks below the surface of the water (the limit swimming time) ) Was measured. In addition, after the breeding, the blood of the mouse was collected, the plasma was separated, and the plasma testosterone value was measured.

  The results are shown in Table 1. From the data in Table 1, black vinegar and comparative samples 1 and 2 did not show significant changes compared to the control group, but sample 1 (black vinegar moromi), phosphatidylserine, sample 2 (black vinegar moromi and phosphatidyl). (Mixture with serine), samples 3, 4 (cereal vinegar), sample 5 (cereal vinegar powder), sample 6 (cereal vinegar mash powder), sample 7 (cereal vinegar powder with mash), sample 8 (cereal vinegar powder with mash) And phosphatidylserine) and sample 9 (a mixture of cereal vinegar mash and phosphatidylserine) have significantly higher plasma testosterone levels and longer marginal swimming time than the control group. I confirmed.

Test Example 2 (Testosterone secretion promotion, anti-fatigue and muscle strengthening test in humans)
Fifty healthy men (21-28 years old, average age: 22.8 years old) who exercise on a daily basis and have consented to participate in the following trials are divided into 11 groups with 5 people per group In each group, gelatin capsules filled with 250 mg of each sample were ingested once a day, and this was continued for 4 weeks. The subject continuously exercises as usual, and blood is collected before and after taking the sample, and a Visual Analog Scale (VAS) questionnaire is conducted as an evaluation of fatigue. The lean mass was measured as follows. In addition, this test is a placebo (indigestible dextrin), the above-mentioned sample 1 (black vinegar mash), phosphatidylserine, sample 2 (mixture of black vinegar mash and phosphatidylserine), sample 5 (cereal vinegar powder), sample 6 (Grain vinegar mash powder), sample 7 (grain vinegar powder with mash), sample 8 (mixture of corn vinegar powder and phosphatidylserine), sample 9 (mixture of grain vinegar mash powder and phosphatidylserine), and Black vinegar and comparative sample 2 were used.

  The results are shown in Tables 2 and 3. In the table, the numerical values are relative values when the value before taking the placebo and the test substance is 100, Table 2 is the average value ± standard error, and Table 3 is the average value (n = 5, ANOVA analysis) ). From the data in Table 2, the plasma testosterone value before and after taking the sample did not change significantly when taking placebo, black vinegar, and comparative sample 2 compared to the values before taking the sample. However, the samples other than black vinegar and Comparative Sample 2 were significantly higher than those before ingestion, confirming the testosterone secretion promoting action.

  The fatigue score in the VAS evaluation also showed the same tendency as the change in the testosterone value. In the samples other than the placebo, black vinegar, and comparative sample 2, the VAS score was significantly reduced, and the anti-fatigue action was confirmed.

  In addition, from the data in Table 3, the amount of lean mass also has the same tendency as the value of the testosterone, and the lean mass was significantly increased in the samples other than placebo, black vinegar and comparative sample 2, and the muscle strengthening action was confirmed. did.

Prototype Example 1: Hard Capsule The above sample 9 was put into a capsule in a filling machine, and a gelatin-coated hard capsule preparation with an internal volume of 250 mg per capsule was made by a conventional method. This capsule preparation can be used as an orally ingestible dietary supplement, pharmaceutical product or animal feed.

Prototype Example 2: Soft Capsule 200 parts of sample 2, 40 parts of beeswax, 50 parts of refined soybean oil are added, mixed by heating, homogenized, and supplied to a capsule filling machine. A gelatin-coated soft capsule formulation was prototyped. This capsule preparation can be used as an orally ingestible dietary supplement, pharmaceutical product or animal feed.

Prototype Example 3: Beverage 1
500 mg of sample 8 was added to 100 mL of a commercially available nutritional drink and mixed well to prepare a beverage. Even when this beverage was stored in a refrigerator for 12 months, no abnormality or discomfort was observed in appearance and flavor. This product can be used as a drink or drink for nourishing tonic.

Prototype Example 4: Beverage 2
50 mL of sample 3 was added to 250 mL of a commercially available sports drink and mixed well to produce a beverage. Even when this beverage was stored in a refrigerator for 12 months, no abnormality or discomfort was observed in appearance and flavor. This product can be used as a beverage or drink to prevent fatigue during exercise or to increase muscles.

Prototype Example 5: Nutritional Supplement 1 g of Sample 1, 1 g of Sample 5, 400 mg of leucine, 250 mg of isoleucine, 200 mg of valine, 300 mg of arginine mixed and sieved, and packaged and filled with a trial product of a dietary supplement . This dietary supplement can also be used for pet food.

  The testosterone secretion promoter comprising the active ingredient of the present invention promotes the secretion of testosterone in the living body by ingestion or administration of the testosterone secretion promoter, so that it can be used for preventing, improving or recovering from fatigue. Sarcopenia, osteopenia, male climacteric disorder, obesity, depression, ED, hair loss, diabetes, coronary artery disease etc. prevention and / or improvement, analgesia, nutrition tonicity, muscle augmentation, and sexual function, endurance It can be used in the form of oral compositions such as foods and drinks, pharmaceuticals, quasi drugs, feeds, pet foods, etc., in applications for improving strength, concentration, sports performance, and the like.

Claims (6)

A testosterone secretion promoter comprising at least one selected from the following (a) to (d) as an active ingredient.
(A) Black vinegar moromi.
(B) Combination of black vinegar moromi and phosphatidylserine.
(C) Grain vinegar obtained from fermentation and aging processes using soybeans and brown rice and / or barley as raw materials.
(D) Grain vinegar obtained through fermentation and aging processes using soybeans and brown rice and / or barley as raw materials.
(E) A combined product of cereal vinegar and phosphatidylserine obtained through fermentation and aging processes using soybeans and brown rice and / or barley as raw materials.
(F) A combination of moromi of cereal vinegar and phosphatidylserine obtained through fermentation and aging processes using soybeans and brown rice and / or barley as raw materials.   The testosterone secretion promoter according to claim 1, wherein the soybean is black soybean.   An anti-fatigue agent comprising the testosterone secretion promoter according to claim 1 or 2.   An oral composition comprising the testosterone secretion promoter according to claim 1 or the anti-fatigue agent according to claim 2.   The oral composition according to claim 4, which is in the form of a food / beverage product, quasi-drug, pharmaceutical product, feed or pet food.   The method for producing cereal vinegar or cereal vinegar moromi according to claim 1, wherein soybean and brown rice and / or barley are fermented simultaneously.