JP2013132288A - Bacterium, complex bacteria for decomposing organic matter, method for treating organic waste, organic fertilizer, method for producing organic fertilizer, and bacterial bed - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a bacterium which can efficiently decompose organic wastes containing fibrous materials such as cellulose or xylan at high temperature, a complex bacteria for decomposing organic matter, a method for treating organic wastes, a bacterial bed, an organic fertilizer capable of reducing its cost and a method for producing the organic fertilizer capable of reducing the cost.SOLUTION: A complex bacteria for decomposing organic matter contains Thermobifida fusca-like I-14 (FERM P-22201) or its mutant, the bacteria, and the bacterium and Bacillus thermochitosensis I-8-5 (FERM P-22202) or its mutant.

Description

  The present invention relates to a bacterium, a complex fungus for decomposing organic matter, an organic waste treatment method, an organic fertilizer, an organic fertilizer production method, and a fungus bed.

  From the viewpoint of environmental protection, interest in organic waste processing using microorganisms is increasing. Examples of organic waste include livestock manure, raw sludge, sludge derived from municipal wastewater, and the like, in addition to raw garbage discarded from homes, restaurants, food processing factories, and the like. Organic waste contains high-molecular substances such as starch and protein. In the organic waste treatment using microorganisms, microorganisms having a degrading activity with respect to such high-molecular substances are used. Such a microorganism decomposes the polymer substance contained in the organic waste, so that the organic waste can be treated.

  Organic waste may include vegetable waste, fallen leaves, rice husks, rice straw and the like. Such organic waste contains a lot of fibers such as cellulose and xylan.

  On the other hand, a method for treating organic waste at a high temperature is known. By treating at a high temperature, water evaporation in the organic waste is promoted, and the organic waste can be quickly reduced in volume. In addition, in organic waste treatment using aerobic bacteria, it is known that the activity of aerobic bacteria decreases when the moisture content in the bacteria bed is high. It is important to maintain the moisture content within 60-65%.

Patent Document 1, wherein a protein, starch, Bacillus bacteria having degradation activity of oil or the like (Bacillus subtilis, Bacillus licheniformis, Bacillus sp.PR15, Bacillus thermodenitrificans) organic waste decomposition method using at 70 ° C. Has been. Patent Document 2 describes a method using a thermus bacterium ( Thermus aquaticus biovar SK542) having a degrading activity of a hardly degradable fibrous substance such as lignin at 75 to 85 ° C. Patent Document 3 describes a method in which the Thermus genus bacterium is combined with a Clostridium thermocellum biovar SK552 having thermophilic cellulolytic activity. Patent Documents 4 and 5 and Non-Patent Document 1 describe a method using YM081 which is capable of growing at 80 ° C. or higher ( Caldothrix satsumae = Calditericola satsumensis ).

JP 2002-58471 A JP-A-6-105679 JP-A-6-191977 JP 2005-013063 A International Publication No. 2003/055985 Pamphlet

Moriya et al. Calditericola satsumensis gen. nov. , Sp. nov. and Calditericola yamamurae sp. nov. , Extreme thermophiles isolated from a high-temperature compost. International Journal of Systemic and Evolutionary Microbiology, 61, 631-636, 2011

  However, in the methods described in Patent Documents 1 to 5 and Non-Patent Document 1, although organic waste can be decomposed at high temperatures using microorganisms, both cellulose and xylan cannot be decomposed, and fibers There were still problems in the treatment of organic waste containing a lot of quality.

  The present invention has been made in view of the above circumstances, and is capable of efficiently decomposing organic waste containing fibers such as cellulose and xylan at high temperature, a complex fungus for decomposing organic matter, and organic waste. It aims at providing a processing method and a microbial bed. Moreover, an object of this invention is to provide the organic fertilizer and organic fertilizer manufacturing method which can be reduced in cost.

In order to achieve the above object, the bacterium according to the first aspect of the present invention is a Thermobifida fusca- like I-14 strain (Accession No. FERM P-22201) or a mutant thereof.

The complex bacterium for decomposing organic matter according to the second aspect of the present invention comprises the bacterium, a Bacillus thermochitosensis I-8-5 strain (accession number FERM P-22202) or a mutant thereof. Including.

  An organic waste treatment method according to a third aspect of the present invention includes a step of bringing the bacterium or the complex fungus for decomposing organic matter into contact with an organic waste.

  The organic waste treatment method may further include a step of performing aeration.

  The organic fertilizer which concerns on the 4th viewpoint of this invention is obtained by the said organic waste processing method, It is characterized by the above-mentioned.

  The organic fertilizer manufacturing method which concerns on the 5th viewpoint of this invention includes the process which the said bacteria or the said complex microbe for organic substance decomposition | disassembly, and organic waste are made to contact.

  The organic fertilizer manufacturing method may further include a step of performing aeration.

  The microbial bed which concerns on the 6th viewpoint of this invention contains the said microbe or the said complex microbe for organic substance decomposition | disassembly.

  According to the present invention, there are provided bacteria capable of efficiently decomposing organic waste containing fibers such as cellulose and xylan at a high temperature, a complex fungus for decomposing organic matter, a method for treating organic waste, and a fungus bed. be able to. Moreover, the organic fertilizer and organic fertilizer manufacturing method which can be reduced in cost can be provided.

It is a top view which shows aeration equipment typically. It is a perspective view which shows aeration equipment typically. It is a diagram showing a systematic position based on neighbor joining from Samobifida fusca (Thermobifida fusca) similar I-14 strain 16S rRNA gene sequence (scale bar = _{0.01 K} nuc.). The numerical value at the branch indicates the confidence value evaluated by bootstrap analysis repeated 1000 times. It is a figure which shows the systematic position based on the neighborhood joint method from the 16S rRNA gene sequence of Bacillus thermochitosensis ( Bacillus thermochitosensis ) I-8-5 strain (scale bar = 0.02 K _nuc .). The numerical value at the branch indicates the confidence value evaluated by bootstrap analysis repeated 1000 times.

  Hereinafter, embodiments of the present invention will be described in detail.

  In the present specification, the organic waste is a waste containing various kinds of organic matter, and is not limited to the following, for example, vegetable scraps discarded from households, restaurants, food processing factories, etc. Food waste including seafood, seafood waste, livestock manure, raw sludge, sludge derived from municipal wastewater, and waste containing a lot of fiber such as fallen leaves and rice straw.

(1. Thermobifida fusca ) Similar I-14 strain or variant thereof
According to the present invention, a Thermobifida fusca- like I-14 strain (hereinafter referred to as I-14) or a mutant of I-14 is provided. I-14 is a related strain of Thermobifida fusca . I-14 has the 16S rRNA gene base sequence shown in SEQ ID NO: 1.

  I-14 has cellulose, xylan, starch, and proteolytic activity. Therefore, by using I-14 for organic waste treatment, organic waste containing a large amount of fiber such as cellulose and xylan can be efficiently decomposed. I-14 is a thermophilic microorganism and has cellulose, xylan, starch, and proteolytic activity even at 55 ° C. or higher. For this reason, I-14 can be used for organic waste processing at high temperature. By performing the organic waste treatment at a high temperature, the evaporation of water in the organic waste is promoted, so that the organic waste can be rapidly reduced. Particularly at 90 to 108 ° C., pathogenic microorganisms, weed seeds and the like contained in the organic waste can be efficiently killed.

  I-14 dated 19th December 2011 at the National Institute of Advanced Industrial Science and Technology Patent Biological Depositary Center (Postal Code 305-8586 Tsukuba Ibaraki City 1-1-1 Tsukuba Center Chuo 6) Deposited at FERM P-22201.

  In the present specification, the I-14 mutant is, for example, 95% or more, preferably 96% or more, 97% or more, 98% or more, or 99% or more, more preferably 100% with the nucleotide sequence shown in SEQ ID NO: 1. It refers to a strain having a 16S rRNA gene base sequence showing% homology. In addition, the I-14 mutant has cellulose, xylan, starch, and proteolytic activity. Furthermore, the I-14 mutant has cellulose, xylan, starch, and proteolytic activity even at 55 ° C or higher.

  A mutant of I-14 can be derived from, for example, I-14 by a mutation treatment known to those skilled in the art. Examples of the mutation treatment include irradiation with radiation such as ultraviolet rays and γ rays, contact with mutagenic chemicals such as methylnitrosourea, N-methyl-N′-nitro-N-nitrosoguanidine (NTG) treatment, ethylmethane A sulfonic acid (EMS) process etc. are mentioned. Any mutation treatment that exhibits the effects of the present invention can be selected as appropriate.

(2. Bacillus thermochitosensis I-8-5 strain or a mutant thereof)
The compound for degrading organic matter according to the present invention includes I-14 or a mutant strain thereof, Bacillus thermochitosensis I-8-5 strain (hereinafter referred to as I-8-5) or a mutant strain thereof. , Is included.

I-8-5 is a novel microorganism belonging to Bacillus thermochitosensis . I-8-5 has the 16S rRNA gene base sequence shown in SEQ ID NO: 2.

  I-8-5 has amylolytic activity. I-8-5 is a thermophilic microorganism and has amylolytic activity even at 55 ° C or higher. For this reason, it can use suitably for the organic waste processing at high temperature similarly to I-14.

  I-8-5 was deposited under the accession number FERM P-22202 on December 19, 2011 at the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology (same as above).

  In the present specification, the I-8-5 mutant is, for example, 95% or more, preferably 96% or more, 97% or more, 98% or more, or 99% or more, more preferably the nucleotide sequence shown in SEQ ID NO: 2. Refers to a strain having a 16S rRNA gene base sequence showing 100% homology. Moreover, the mutant of I-8-5 has amylolytic activity. Furthermore, the mutant of I-8-5 has amylolytic activity even at 55 ° C or higher.

  A mutant of I-8-5 can be derived from, for example, I-8-5 by the same mutation treatment as described above.

(3. Isolation and identification of strains)
Each of the above-mentioned strains includes, for example, organic waste (food waste including vegetable scraps, food waste from food factories, fishery waste, etc.) on wood chips cut from broad-leaved trees and conifers cut off near Chitose City, Hokkaido. , Livestock manure, rice straw, etc.) repeatedly and maintained at 90 ° C. to 108 ° C., it can be isolated and identified from the high temperature bacterial bed formed.

  As a method for isolating and identifying each of the above-mentioned strains, for example, a microorganism having the ability to grow at 65 ° C. or higher from the above-mentioned high-temperature bacterial bed and having the activity of degrading cellulose, xylan, starch, and protein And then determining the taxonomic position of the strain by analyzing the homology of the 16S rRNA gene base sequence. Any isolation and identification method that exhibits the effects of the present invention can be selected as appropriate.

  The cellulolytic activity of the strain is, for example, inoculating the isolated strain on an agar medium containing carboxymethylcellulose, culturing, and then staining with Congo Red or the like to observe the presence or absence of a clear zone around the colony It is evaluated by. Any evaluation method that exhibits the effects of the present invention can be selected as appropriate.

  The xylan-degrading ability of the strain can be determined by, for example, inoculating the isolated strain on an agar medium containing xylan, culturing, and then staining with Congo Red etc., and observing the presence or absence of a clear zone around the colony. Be evaluated. Any evaluation method that exhibits the effects of the present invention can be selected as appropriate.

  Starch resolution of strains is evaluated by, for example, inoculating the isolated strain on an agar medium containing starch, culturing and then staining with Lugol's solution, etc., and observing the presence or absence of a clear zone around the colony Is done. Any evaluation method that exhibits the effects of the present invention can be selected as appropriate.

  The protein resolution of the strain is evaluated by, for example, inoculating the isolated strain on an agar medium containing casein and culturing, and then observing the presence or absence of a clear zone around the colony. Any evaluation method that exhibits the effects of the present invention can be selected as appropriate.

  Regarding the homology of the gene base sequence, for example, a BLAST search published on the homepage (http://www.ddbj.nig.ac.jp/Welcome-j.html) of DNA Data Bank of Japan (DDBJ) or It can be determined using DNA sequence analysis software such as GENETYX (Genetics Co., Ltd.). Any method that exhibits the effects of the present invention can be selected as appropriate.

(4. Complex bacteria for organic matter decomposition)
As described above, the complex bacterium for decomposing organic matter according to the present invention further includes I-8-5 or a mutant thereof in addition to I-14 or a mutant thereof. As described above, I-14 or a mutant thereof has cellulose, xylan, starch, and proteolytic activity, and I-8-5 or a mutant thereof has amylolytic activity. For this reason, by using the complex bacteria for decomposing organic matter for treating organic waste, it is possible to decompose fibers such as cellulose and xylan, and to further decompose organic waste more efficiently due to the synergistic effect of starch degrading activity. be able to. Further, as described above, these strains have the above-described degradation activity even at 55 ° C. or higher. For this reason, this complex microbe for organic substance decomposition | disassembly can be used for the organic waste process at high temperature. By performing the organic waste treatment at such a high temperature, the evaporation of moisture in the organic waste is promoted, so that the organic waste can be rapidly reduced. Particularly at 90 to 108 ° C., pathogenic microorganisms, weed seeds and the like contained in the organic waste can be efficiently killed.

(5. Bacteria bed)
The fungal bed according to the present invention contains I-14 or a mutant strain thereof, or a complex fungus for decomposing organic matter according to the present invention. The fungus bed is, for example, in the form of a soil, a block, or the like, and is used for organic waste treatment at 55 ° C. or higher, preferably 90 to 108 ° C. The size of the fungus bed is, for example, a width of 10 m, a depth of 10 m, and a height of 2 to 3 m.

(6. Organic waste disposal method)
The organic waste processing method according to the present invention includes a step of bringing the organic waste into contact with I-14 or a mutant strain thereof or the complex fungus for decomposing organic matter according to the present invention. In the organic waste treatment method, for example, the organic waste is decomposed by introducing the organic waste into the above-mentioned fungus bed and bringing the complex fungus for organic matter decomposition into contact with the organic waste. .

  As described above, I-14 or a mutant strain thereof, or the complex fungus for decomposing organic matter can degrade cellulose, xylan, starch, and protein, so that cellulose, xylan, etc. can be obtained by the organic waste treatment method. Organic waste containing a large amount of fiber can be efficiently decomposed. In addition, I-14 or a mutant strain thereof, or the complex fungus for decomposing organic matter can be used for organic waste treatment at 55 ° C. or higher as described above. For this reason, evaporation of water in the organic waste is promoted, and the organic waste can be rapidly reduced. Particularly at 90 to 108 ° C., pathogenic microorganisms, weed seeds and the like contained in the organic waste can be efficiently killed.

  The organic waste treatment method according to the present invention may further include a step of performing aeration. As a method of aeration, for example, a method of sending air to the microbial bed using a blower can be mentioned. In this method, for example, a plurality of pipes provided with a plurality of air holes are installed on the floor of the fungus bed, and the pipes are connected to a blower so that air is supplied to the fungus bed through the air holes of the pipes. Send it in. Any aeration method that exhibits the effects of the present invention can be selected as appropriate. By performing aeration, oxygen can be continuously supplied to aerobic bacteria I-14 or a mutant thereof and I-8-5 or a mutant thereof. As a result, since the activity of these strains can be increased, organic waste can be decomposed more efficiently. Moreover, since the water | moisture content contained in organic waste can be evaporated more effectively, the organic waste can further be reduced rapidly.

  The case where organic waste, such as raw garbage, fishery waste, and livestock excreta containing vegetable waste having a high moisture content, is treated using the organic waste treatment method according to the present invention will be described. For example, 2 to 3 tons of the organic waste is put into a fungus bed containing I-14 and I-8-5 every day, and aeration is always performed. The microbial bed can be maintained at, for example, 90 ° C. to 108 ° C. without applying heat from the outside. I-14 and I-8-5 efficiently decompose cellulose, xylan, starch, and protein in the organic waste, and the organic waste is rapidly reduced by treatment at high temperature. Can be achieved.

  For example, the case of treating livestock manure mixed with bedding (made of rice straw, sawdust, rice husk, etc., and containing a lot of fibers such as cellulose, xylan) using the organic waste treatment method according to the present invention will be described. To do. For example, the livestock manure is put into a fungus bed containing I-14 and I-8-5 every day, and aeration is always performed. Moreover, since livestock manure is highly viscous, the fungus bed is simply agitated about once every 2 to 3 months with a tire shovel or the like for the purpose of increasing the chance of contact between livestock manure and these strains. The microbial bed can be maintained at, for example, 90 ° C. to 108 ° C. without applying heat from the outside. I-14 and I-8-5 efficiently decompose cellulose, xylan, starch, and protein in the organic waste, and the organic waste is rapidly reduced by treatment at high temperature. Can be achieved. By this method, it is possible to continuously treat livestock manure for about one year.

(7. Organic fertilizer)
According to the present invention, an organic fertilizer is provided. The organic fertilizer can be obtained by using the organic waste treatment method described above. Since the organic fertilizer can be obtained by decomposing organic waste that has only been discarded, costs can be reduced. Moreover, the organic fertilizer obtained by decomposing | degrading naturally derived organic wastes, such as a plant and an animal, can avoid the problem of environmental pollution which is anxious about use of a chemical fertilizer.

(8. Organic fertilizer manufacturing method)
According to the present invention, a method for producing an organic fertilizer is provided. The method for producing organic fertilizer includes a step of bringing I-14 or a mutant strain thereof, or a complex bacterium for decomposing organic matter according to the present invention, and an organic waste. In the organic fertilizer manufacturing method, the organic fertilizer can be manufactured by decomposing the organic waste that has only been discarded, so that the cost can be reduced. In addition, the organic fertilizer manufacturing method may further include a step of aeration. In this case, since organic waste can be decomposed more efficiently, organic fertilizer can be manufactured more quickly. it can.

  Hereinafter, the present invention will be specifically described with reference to examples. However, the present invention is not limited to these examples.

(Segregation and identification of strains)
The strain was isolated from a high-temperature bacterial bed formed in Chitose City, Hokkaido. This high-temperature fungus bed is made from wood chips that are cut into hardwoods and conifers that have been cut off in the suburbs of Chitose City, Hokkaido to a length of 40 mm or less and a diameter of 10 mm or less, raw garbage containing vegetable scraps, food waste from food factories, and fishery waste. And organic waste such as livestock excreta were repeatedly introduced for about 10 years. On the way, a part of the high temperature bacterial bed is newly allocated on the floor 110 (FIGS. 1 and 2) of the aeration equipment 100 and mixed with the wood chip and the organic waste, thereby Continued.

  The aeration equipment 100 includes a floor 110, a blower 120 (trade names “VFC602A” and “VFC705A” manufactured by Fuji Electric Co., Ltd.), a main pipe 130, a branch pipe 140, and a concrete wall 150 (FIGS. 1 and 2). The floor 110 was 10 m wide and 10 m deep. The height of the concrete wall 150 was about 10 m. The main pipe 130 is an iron pipe having a diameter of 8 to 10 cm, and is connected to the blower 120. The main pipe 130 was installed in a groove provided in the floor 110 of the aeration equipment 100. The branch pipe 140 is a rubber pipe having a diameter of 4 cm, and a plurality of branch pipes 140 are connected to the main pipe 130. The branch pipe 140 was provided with air holes (not shown) having a diameter of 5 mm at intervals of 50 cm. Similar to the main pipe 130, the branch pipe 140 was installed in a groove provided in the floor 110 of the aeration equipment 100. The concrete wall 150 was about 10 cm thick. The concrete wall 150 was provided with an opening.

  The high temperature bacteria bed was always piled up on the floor part 110 of the aeration equipment 100 to a height of about 2 to 3 m (FIG. 2). The air sent from the blower 120 was sent to the branch pipe 140 via the main pipe 130, and was always sent to the high-temperature bacteria bed in the direction of arrow W through the air hole of the branch pipe 140. Aeration was performed continuously. The organic waste was put in 2 to 3 tons from an opening of the aeration equipment 100 every day by a shovel car or the like. The temperature inside the high-temperature bacteria bed was maintained at 90 ° C. to 108 ° C. even in winter without applying heat from the outside. The high-temperature bacteria bed thus formed was used as a sample.

5 mL of sterilized water was added to a sterile disposable container containing 5 mL of the above sample to dilute the sample. This was pH 8.0 PYG medium (8 g peptone, 3 g yeast extract (product code: 551-01310-8), 1 g dipotassium phosphate (K ₂ HPO ₄ ), gellan gum (Wako Pure Chemical Industries, Ltd.) Manufactured product code: 075-03075) was dissolved in 1000 mL of deionized water) and cultured at 60 ° C. for 24 hours. Isolation and purification were repeated by separating colonies that grew on the medium, inoculating the PYG medium, and culturing. Two strains of DNA obtained as a result of isolation and purification were extracted. The 16S rRNA gene was amplified by PCR using 9F primer (SEQ ID NO: 3) and 1541R primer (SEQ ID NO: 4), and 16S using an autosequencer (product name “PRISM 3100” manufactured by Applied Biosystems). The base sequence of the gene encoding the rRNA base sequence was analyzed. Their base sequences are shown in SEQ ID NOs: 1 and 2.

Based on the base sequence shown in SEQ ID NO: 1, by BLAST search published on the homepage of DNA Data Bank of Japan (DDBJ) (http://www.ddbj.nig.ac.jp/Welcome-j.html) As a result of homology search, the base sequence shown in SEQ ID NO: 1 showed the highest homology (98.9%) with Thermobifida fusca . The strain having the 16S rRNA gene base sequence shown in SEQ ID NO: 1 was designated as I-14. FIG. 3 shows a systematic relationship with related species based on the 16S rRNA base sequence of I-14.

As a result of homology search based on the base sequence shown in SEQ ID NO: 2 in the same manner as described above, the strain having the 16S rRNA gene base sequence of SEQ ID NO: 2 is a novel strain of Bacillus thermochitosensis ( Bacillus thermochitosensis ). found. This strain was designated as I-8-5. FIG. 4 shows a systematic relationship with related species based on the 16S rRNA base sequence of I-8-5.

  Tables 1 and 2 show the mycological properties of the above two strains.

(Evaluation of degradation activity of cellulose, xylan, starch, and protein)
The degradation activity of cellulose, xylan, starch, and protein of I-14 and I-8-5 was evaluated.

(1) Evaluation of cellulolytic activity pH 8.0 agar medium (peptone 10 g / L, yeast extract 3) containing 0.5% carboxymethylcellulose (CMC) (manufactured by Wako Pure Chemical Industries, Ltd., product code: 039-01335) 0-14 (containing 0 g / L, CMC 5 g / L, and agar 15 g / L), each of which was inoculated with I-14 and I-8-5 and cultured at 55 ° C. for about 1 week. After culturing, 0.1% Congo red aqueous solution was overlaid and placed at room temperature for 30 minutes. Then, 1M NaCl was overlaid for 30 minutes, and the medium was washed by removing NaCl. Washing with 1M NaCl was repeated several times. A case where a yellow clear zone was formed around the colony was determined to be positive for cellulolytic activity. As a result, cellulolytic activity was observed in I-14.

(2) Evaluation of xylan decomposition activity In place of the above CMC 0.5%, an agar medium containing 0.5% xylan (manufactured by Sigma, product code: x0627-25G) was added with I-14 and I-8-5. Each was inoculated and cultured at 55 ° C. for about 1 week. After culturing, staining and washing were performed in the same manner as described above. A case where a yellow clear zone was formed around the colony was determined to be positive for xylan decomposition activity. As a result, xylan decomposition activity was observed in I-14.

(3) Evaluation of amylolytic activity In an agar medium containing 0.5% soluble starch (product code: 196-13185) instead of CMC 0.5%, I-14, I- Each of 8-5 was inoculated and cultured at 55 ° C. for about 1 week. After the cultivation, Lugol's solution that specifically stains starch was layered to stain starch remaining in the medium. When a clear zone was formed around the colony due to the decomposition of starch, it was determined that the starch decomposition activity was positive. As a result, amylolytic activity was observed in I-14 and I-8-5.

(4) Evaluation of proteolytic activity pH 8.0 agar medium (peptone 10 g / L, yeast extract 3.0 g / L, casein 10 g / L) containing 1% casein (manufactured by Morinaga Milk Industry Co., Ltd., trade name “Skim Milk”) , And 15 g / L of agar) were inoculated with I-14 and I-8-5, respectively, and cultured at 55 ° C. for about 1 week. After culturing, the case where a clear zone was formed around the colony due to degradation of casein was determined to be positive for proteolytic activity. As a result, proteolytic activity was observed in I-14.

  From the above, it was shown that I-14 has cellulose, xylan, starch, and proteolytic activity, and I-8-5 has amylolytic activity.

  As described above, according to the present invention, bacteria capable of efficiently decomposing organic waste containing fibers such as cellulose and xylan at high temperatures, complex fungi for decomposing organic matter, organic waste treatment method, And a fungal bed can be provided. Moreover, the organic fertilizer and organic fertilizer manufacturing method which can be reduced in cost can be provided.

100 Aeration equipment 110 Floor 120 Blower 130 Main pipe 140 Branch pipe 150 Concrete wall

Claims (8)

A bacterium which is a Thermobifida fusca- like I-14 strain (accession number FERM P-22201) or a mutant thereof. The bacterium of claim 1;
Bacillus thermochitosensis I-8-5 strain (Accession No. FERM P-22202) or a mutant thereof,
including,
A complex fungus for decomposing organic matter. A step of bringing the bacterium according to claim 1 or the complex bacterium for decomposing organic matter according to claim 2 into contact with an organic waste,
A method for treating organic waste. A further step of performing aeration,
The organic waste processing method according to claim 3, wherein: Obtained by the organic waste treatment method according to claim 3 or 4,
Organic fertilizer characterized by that. A step of bringing the bacterium according to claim 1 or the complex bacterium for decomposing organic matter according to claim 2 into contact with an organic waste,
The organic fertilizer manufacturing method characterized by the above-mentioned. A further step of performing aeration,
The method for producing an organic fertilizer according to claim 6.   A microbial bed comprising the bacterium according to claim 1 or the complex bacterium for decomposing organic matter according to claim 2.

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