JP2013102762A - Yeast fermented feed and method for producing the same - Google Patents

Yeast fermented feed and method for producing the same Download PDF

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JP2013102762A
JP2013102762A JP2011260958A JP2011260958A JP2013102762A JP 2013102762 A JP2013102762 A JP 2013102762A JP 2011260958 A JP2011260958 A JP 2011260958A JP 2011260958 A JP2011260958 A JP 2011260958A JP 2013102762 A JP2013102762 A JP 2013102762A
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fermented feed
dough
yeast
okara
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Tamio Kobayashi
民男 小林
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BIITEIN KENKYUSHO KK
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract

PROBLEM TO BE SOLVED: To establish yeast fermented feed of high quality which is improved in digestion and absorption with excellent taste, particularly improved in taste for pigs, using bean curd lees.SOLUTION: The yeast fermented feed produced by yeast fermentation of bean curd lees is easily produced by preparing optimum dough, and carrying out not anaerobic fermentation but semi-anaerobic fermentation in order not to cause abnormal fermentation. Further, sweetness is imparted to the dough in order to improve the taste for establishment.

Description

大豆加工工場〈豆腐、油揚げ、豆乳製造等〉から大量に出るおからを有効な資源として利用せんとするもので、適正なる条件を設定する事によって、醗酵飼料とし、嗜好性と可消化養分総量をあげ、特に、嗜好性の高い豚用の飼料を容易に作る事が出来るおからの酵母醗酵飼料の製造方法に関する。A large amount of okara from soybean processing plants (tofu, fried tofu, soy milk production, etc.) is used as an effective resource. By setting appropriate conditions, fermented feed, palatability and total digestible nutrients In particular, the present invention relates to a method for producing a fermented yeast fermented feed that can easily make a feed for pigs with high palatability.

近年、工場の生産規模は大規模化して来ており、おからも単位工場で大量に排出される様になってきた。それに伴い、大半のメーカーではおからの乾燥処理を進めて来たが、大型設備とランニングコストがかさみ、採算が取れない状態となって来ている。
一方小中規模メーカーの一部では、そのまま飼料にしたり、肥料としているが問題を抱えており、全体的にみて、今だに良い処理方法は見出せていない。
おからは水溶性成分の多くが抽出された残渣であり、粗蛋白、粗脂肪、可消化性養分に富むものの、繊維質が多く、消化吸収の点で良好であるとは言えない。
そして、おからは水分が多く、排出の温度が高いため、特に夏場は耐熱性菌や雑菌の繁殖しやすい環境にある。
これを直接飼料に向けた場合、異臭で、食いつきが悪いばかりか、家畜の消化器系の疾患を引き起こす事も稀ではない。
おからは日本国内では、凡そ年間50万トン程度の大量の食品残渣として排出されている。
In recent years, the production scale of factories has been increasing, and okara also has been discharged in large quantities at unit factories. Along with that, most manufacturers have been proceeding with the drying process of okara, but large equipment and running costs are increasing, making it unprofitable.
On the other hand, some small and medium-sized manufacturers use it as it is or feed it as fertilizer, but there are problems, and as a whole, no good treatment method has been found yet.
Okara is a residue from which most of the water-soluble components have been extracted. Although it is rich in crude protein, crude fat, and digestible nutrients, it is rich in fiber and cannot be said to be good in terms of digestion and absorption.
And since okara has a lot of water and the temperature of discharge is high, it is in an environment where heat-resistant bacteria and bacteria are easy to propagate, especially in summer.
When this is applied directly to the feed, it is not uncommon for it to have a bad odor, poor bite, and cause diseases of the digestive system of livestock.
Okara is discharged in Japan as a large amount of food residue of about 500,000 tons per year.

おからをそのまま家畜飼料として利用するのは次のような点で問題がある。
▲1▼含水率が75%乃至85%あり、日持ちしない。
▲2▼含水率が高いので、取扱が容易ではない。
▲3▼家畜の嗜好性が劣り、好んでは食べない、特に豚は最たるものがある。
▲4▼繊維を多く含むので、消化率が低い、従って、家畜は消化不良を起し易くなり、強いては消化器系の疾患を起しやすくなる。
Using okara as a livestock feed has the following problems.
(1) It has a moisture content of 75% to 85% and will not last for a long time.
(2) Since the moisture content is high, handling is not easy.
(3) The taste of livestock is inferior, and it is not preferred to eat, especially pigs.
{Circle around (4)} Since the fiber contains a large amount of fiber, the digestibility is low. Therefore, livestock is liable to cause indigestion, and thus easily causes digestive diseases.

おからを有効利用しようとする試みは以前から行われている。例えば、特許文献1では、先ず醗酵飼料にし、之を堆肥化する事が提案されている。Attempts to effectively use okara have been made for some time. For example, Patent Document 1 proposes that a fermented feed is first used and composted.

脱脂大豆、おからを乳酸菌で醗酵させたものを飼料とする試みも行なわれている。
特許文献2には、おからと大量のフイチン分解作用のある小麦破砕物との混合物を乳酸醗酵させることにより、醗酵飼料を製造する方法が提案されている。
Attempts have been made to use defatted soybeans and okara fermented with lactic acid bacteria as feed.
Patent Document 2 proposes a method for producing a fermented feed by lactic acid fermentation of a mixture of okara and a large amount of crushed wheat having phytine-decomposing action.

特許文献3に見るように、おからにビートパルプ等の糟糠類を加え水分調整した上で、繊維分解酵素を加えて、消化性及び嗜好性を上げた醗酵飼料を製造する方法が提案されている。As seen in Patent Document 3, a method has been proposed in which fermented foods such as beet pulp are added to okara and moisture is adjusted, and then a fiber-degrading enzyme is added to produce a fermented feed that has improved digestibility and palatability Yes.

豆腐を製造の際に出来る“ゆ”に、乳酸菌を静置培養し、それにおからを加え、24時間静置培養する。然る後、醗酵したおからを濾過する方法でつくる醗酵飼料を特許文献4で提案されている。Lactic acid bacteria are statically cultured in “yu” that can be produced during the production of tofu, added to it, and then statically cultured for 24 hours. Thereafter, Patent Document 4 proposes a fermented feed made by a method of filtering fermented okara.

おからをパン酵母とグルコースの混合物で覆い一方弁機能弁を取り付け、アルコール醗酵させて、おからを日持ちさせる方法が特許文献5で提案されている。Patent Document 5 proposes a method of covering okara with a mixture of baker's yeast and glucose, attaching a valve function valve, fermenting alcohol, and keeping okara for a long time.

おからを天然酵母で醗酵させ、醗酵期間が2日乃至10日を要する処理方法で、醗酵飼料をつくる方法が特許文献6で提案されている。Patent Document 6 proposes a method of fermenting okara with natural yeast and producing a fermented feed by a treatment method that requires 2 to 10 days for the fermentation period.

PHを4〜5に調節し、水分を77%以下に脱水し、嫌気醗酵してサイレージ化する方法が特許文献7で提案されている。
特開平8−217585 特開平9−140334 特開平9−51762 特開2005−261390 特開2006−197809 特開平6−169700 特開昭59−213366
Patent Document 7 proposes a method of adjusting the pH to 4 to 5, dehydrating water to 77% or less, and performing anaerobic fermentation to silage.
JP-A-8-217585 JP-A-9-14334 JP-A-9-51762 JP-A-2005-261390 JP2006-197809 JP-A-6-169700 JP 59-213366

等々提案されている。しかしながら、これ等の方法は特別な装置が必要であったり、操作が複雑、面倒であったり、更に原料、材料を大量に使用するため高原価となったりして、いま一歩実用化されていない。And so on. However, these methods are not put into practical use yet because special equipment is required, the operation is complicated and troublesome, and the cost is high due to the large amount of raw materials and materials used. .

大量に出るおからをサイレージにすることは既に、低原価おからサイレージの製造方法、で提案しているが、しかしサイレージは乳酸醗酵させてなるもので、当然酸臭、酸味があり、反芻動物は好んで食べるが、単胃動物の豚は酸臭、酸味を忌避して食べない。
そこで、豚の嗜好性が高い、おからの酵母醗酵飼料を創出するものである。
特別な装置を必要とせず、操作は簡単であり、醗酵は早く、製品は長期に保管が出来る、おから酵母醗酵飼料の製造方法を提供するものであり、
畜産飼料として用いた場合、豚、牛の嗜好性は良く、特に豚の嗜好性は顕著なものがある、消化吸収は良く、栄養価も高く、増体効果が出る。
該飼料の畜産業、特に、養豚業への用途を提供しようとするものである。
Producing silage from large quantities of okara has already been proposed in the low-cost production method of okara silage, but silage is a product of lactic acid fermentation and naturally has an acid odor and acidity, and is a ruminant. I prefer to eat, but monogastric pigs avoid acidity and acidity and do not eat.
Therefore, it is intended to create a yeast fermented fermented okara that has a high palatability for pigs.
No special equipment is required, the operation is simple, the fermentation is fast, and the product can be stored for a long period of time, providing a method for producing okara yeast fermented feed,
When used as livestock feed, the palatability of pigs and cattle is good, especially the palatability of pigs is remarkable, digestion and absorption are good, nutritional value is high, and a body weight gain effect is obtained.
The present invention intends to provide uses of the feed to the livestock industry, particularly to the pig farming industry.

課題を解決する為の手段Means to solve the problem

上記の問題を解決する為には、おからに水分調整剤として、濃厚飼料又は糟糠類を加え、調整した上で、適量の有機酸とブドウ糖を加え、更に酵母菌を加えて、生地の調整を行なう。調整出来たものを準嫌気的雰囲気にして、醗酵させると上記課題が解決できる。To solve the above problems, add concentrated feed or potatoes as a moisture regulator to the okara, adjust it, add an appropriate amount of organic acid and glucose, add yeast, and adjust the dough. To do. The above-mentioned problem can be solved by making the adjusted product a semi-anaerobic atmosphere and fermenting it.

本発明に用いるおからは、出来るだけ新鮮な物が好ましく、製造後数時間内のもとし、10時間以上経過した物は往々にして酸敗が起っており、PHも低下しておる、腐敗臭もする。この様なおからは目的の酵母醗酵には適さない。The okara used in the present invention is preferably as fresh as possible, and within a few hours after production, the product that has passed 10 hours or more often suffers from rancidity and has a low pH. It also smells. Such okara is not suitable for the intended yeast fermentation.

本発明に用いるPH調整はPHを下げる事によって雑菌繁殖を抑制するもので、PH調整剤は飼料添加物で認められているものであれば良い、PH調整が軽微であるので、液状有機酸でも差し支えない。
雑菌の繁殖を抑える為、生地のPHを6以下にする必要がある。一方4以下にすると酵母醗酵を阻害することになり、酸味酸臭を生じるので不適である。
The PH adjustment used in the present invention suppresses miscellaneous bacterial growth by lowering the PH, and the PH adjuster may be any approved as a feed additive. Since the PH adjustment is slight, even a liquid organic acid may be used. There is no problem.
In order to suppress the propagation of germs, the pH of the dough needs to be 6 or less. On the other hand, if it is 4 or less, yeast fermentation is inhibited, and a sour acid odor is generated, which is not suitable.

本発明で醗酵飼料の生地水分は60乃至70重量%にするのが望ましく、60重量%以下にすれば、原価コストが高くなり、70重量%以上にすれば、異常醗酵が起りやすくなって、往々にして、悪臭がするようになる。悪臭、酸臭は豚飼料としては避けなければならない。In the present invention, the dough moisture of the fermented feed is desirably 60 to 70% by weight, and if it is 60% by weight or less, the cost is increased, and if it is 70% by weight or more, abnormal fermentation is likely to occur. Often, it becomes odorous. Odor and acid odor must be avoided for pig feed.

本発明に用いるおからの水分調整剤は糟糠類にはビートパルプ、ふすま、米糠、コーンコブミール等がある。一方濃厚飼料にはトウモロコシ、グレインソルガム、小麦、大麦、玄米、大豆等が有る。この中で、入手が容易で、安定的で、価格も低廉でなければ成らない。従って糟糠類ではコーンコブミール、ビートパルプミール、濃厚飼料では大麦ミールを用いるのが好適である。Okara moisture regulators used in the present invention include beet pulp, bran, rice bran, corn cob meal and the like. On the other hand, concentrated feed includes corn, grain sorghum, wheat, barley, brown rice, soybeans and the like. Among these, it must be easy to obtain, stable, and inexpensive. Therefore, it is preferable to use corn cob meal, beet pulp meal for barley, and barley meal for concentrated feed.

本発明に用いる糖類としてはブドウ糖、蔗糖があるが、中でも単糖類のブドウ糖が資源として有効に取り込まれるので好ましい。The sugars used in the present invention include glucose and sucrose. Among them, monosaccharide glucose is preferable because it is effectively incorporated as a resource.

本発明に用いる酵母菌は取り扱いの煩雑を避けるために、ドライ酵母を使用する事が望ましい。
添加量は生地に対し、1重量%以上の添加ではコストが掛かり、また0.1%重量以下では少なくて酵母醗酵しない。
菌種は嗜好性が要求されるので、清酒酵母、ビール酵母ではなく、パン酵母を用いる。酵母菌種は次のものを用いる。
Saccharomyces cerevisiae
As the yeast used in the present invention, it is desirable to use dry yeast in order to avoid complicated handling.
The addition amount is 1% by weight or more with respect to the dough, and it costs less than 0.1% by weight or less, and does not ferment yeast.
Bacterial species require palatability, so baker's yeast is used instead of sake yeast or beer yeast. The following yeast species are used.
Saccharomyces cerevisiae

以下、実施例を参照しながら、本発明をより詳しく説明するが、本発明が実施例に限定されない事は言うまでも無い。Hereinafter, the present invention will be described in more detail with reference to examples. However, it goes without saying that the present invention is not limited to the examples.

飼料では嗜好性も重要な要素で、特に豚では、腐敗や酸敗での酸臭、酸味は嫌い、食べない。乳酸醗酵させたおからサイレージも同様である。
一方、甘味、甘臭は嗜好性を多いに高めるもので、飼料の甘味甘臭は必須であって、そのウェイト大きい。しかし糖類等を大量に添加する事は醗酵に著しい影響を与えるばかりか、微生物の資源として利用され、甘味の付与には貢献しない場合がある。又コスト面でも経済的な負担は大きい。
従って、甘味を付与する為には、飼料添加物として許可されている呈味料のサッカリンナトリウムを使用し、生地に対し0.005重量%乃至0.05重量%添加することが最適である。
In feed, palatability is also an important factor, especially in pigs. The same applies to okara silage fermented with lactic acid.
On the other hand, sweetness and sweet odor increase the palatability, and the sweet and sweet odor of feed is essential and its weight is large. However, adding a large amount of sugar or the like not only significantly affects fermentation, but is also used as a microbial resource and may not contribute to imparting sweetness. In terms of cost, the economic burden is large.
Accordingly, in order to impart sweetness, it is optimal to use saccharin sodium, a flavoring agent permitted as a feed additive, and to add 0.005% to 0.05% by weight to the dough.

おからを酵母醗酵させる際、酵母菌と同じ真菌類の黴が繁殖しやすい、之を防止する為には嫌気的状態にする事であるが、嫌気的にする事はサイレージを作る事になるので、完全嫌気状態には出来ない。
従って、準嫌気状態とし、一方防黴剤のプロピオン酸カルシウムを0.3重量%〜1.5重量%使用して、黴の発生を防ぐ事が必要である。しかし、大量に使用すると酵母菌の繁殖も阻害する。
準嫌気状態にするには、出来るだけ空気は取り除いた後、完全密封しないで封鎖するに止める。従って、系より発生したCO、エタノール等は醗酵容器より排出されされるが、空気の出入は無い状態とする事が望ましい。
When yeast is fermented from okara, it is easy to propagate the same fungi moth as yeast. To prevent this, it is necessary to make it anaerobic, but making it anaerobic will make silage. So it can not be completely anaerobic.
Therefore, it is necessary to prevent the generation of soot by using a semi-anaerobic state while using 0.3 to 1.5% by weight of calcium propionate as an antifungal agent. However, when used in large quantities, it also inhibits the growth of yeast.
To make a semi-anaerobic condition, remove as much air as possible and then seal it without sealing it completely. Therefore, it is desirable that CO 2 , ethanol, etc. generated from the system are discharged from the fermentation vessel, but no air enters or leaves.

防黴剤の必要性とその添加量について検討した。
おからを製造直後に4、000g採取した、そのおからの含水量は75.4%であった。
それに糟糠類のコーンコブミールを600g加え、良く混合して、水分調整した。
調整後の水分は66.4%であつた。
それに、87重量%の蟻酸を3.7ml、ブドウ糖46g、イースト菌25.5g加え均一に混合し、2Lポリ袋に各々900g入れ、それにプロピオン酸カルシウムを各、a;2.9g ,b;3.8g ,c;4.9g ,d;6.4g ,e;8.3g 、加え良く混合した。
きつく口を絞って、準嫌気状態(新しい空気は入らないが、発生した炭酸ガス、エチルアルコールは排出可能な状態とする)にし、8日間醗酵させた(醗酵温度20〜29℃)。
その結果はつぎの通であつた。

Figure 2013102762
The necessity and amount of antifungal agent were examined.
4,000 g of okara was collected immediately after production, and the water content from the okara was 75.4%.
600 g of corn cob meal was added to it and mixed well to adjust the water content.
The water content after adjustment was 66.4%.
To this, 3.7 ml of 87% by weight of formic acid, 46 g of glucose, and 25.5 g of yeast were added and mixed uniformly, and each 900 g was put into a 2 L plastic bag, and calcium propionate was added to each a; 2.9 g, b; 8 g, c; 4.9 g, d; 6.4 g, e; 8.3 g, and mixed well.
The mouth was squeezed into a semi-anaerobic state (no new air entered, but the generated carbon dioxide and ethyl alcohol could be discharged) and fermented for 8 days (fermentation temperature 20-29 ° C.).
The result was as follows.
Figure 2013102762

実験結果の評価
プロピオン酸カルシウムの黴発生防止効果は何れの添加量のもでも、あつたが腐敗臭、異臭が全く感じられないものとなると調整生地に対し0.7%以上の添加量のものでなければ成らない事が分った。
Evaluation of the experimental results Calcium propionate has the effect of preventing wrinkle generation at any added amount. I knew that I had to do it.

飼料の嗜好性を上げるため、特に豚用の嗜好性を上げるため、甘味料の添加が不可欠であり、飼料添加物で認められている甘味料(サツカリンナトリウム)の添加の有意性について検討した。
おからを製造直後に4、600g採取した、そのおからの含水量は74.4%であった。
それに濃厚飼料の大麦ミールを700g加え、良く混合して、水分調整した。
調整後の水分は67.6%であつた。
それに、87重量%の蟻酸を4.2ml、ブドウ糖53g、イースト菌29.2gプロピオン酸カルシウム37.1g加え均一に混合し、2Lポリ袋に各々800g入れ、それにサッカリンナトリウムを各、イ;0 ,ロ;0.04g ,ハ;0.08g ,ニ;0.27g ,ホ;0.54g 、ヘ;0.8g 加え良く混合した。
きつく口を絞って、準嫌気状態(新しい空気は入らないが、発生した炭酸ガス、エチルアルコールは排出可能な状態とする)にし、14日間醗酵させた(醗酵温度18〜29℃)。
その結果は次の通であつた。

Figure 2013102762
In order to improve the palatability of feed, especially for pigs, the addition of sweeteners is indispensable, and the significance of the addition of a sweetener (satsukarin sodium) that is recognized as a feed additive was examined. .
4,600 g of okara was collected immediately after production, and the water content from the okara was 74.4%.
700g of concentrated barley meal was added thereto and mixed well to adjust the water content.
The moisture after adjustment was 67.6%.
To this, 4.2 ml of 87% by weight of formic acid, 53 g of glucose, 29.2 g of yeast, 37.1 g of calcium propionate, and uniformly mixed, put 800 g of each in a 2 L plastic bag, and add saccharin sodium to each of A, B; 0.04 g, c; 0.08 g, d; 0.27 g, e; 0.54 g, f; 0.8 g.
The mouth was tightly squeezed into a semi-anaerobic state (no new air entered, but the generated carbon dioxide and ethyl alcohol could be discharged) and fermented for 14 days (fermentation temperature 18-29 ° C.).
The results were as follows.
Figure 2013102762

実験結果の評価
豚の嗜好性を示すものは、甘味料(サツカリン酸ナトリウム)の若干の添加量でも示すが、競って食べるのは調整生地に対し0.03%以上の添加量である必要がある事が分かつた。
Evaluation of experimental results What shows the palatability of pigs is also indicated by a slight addition amount of sweetener (sodium saccharinate), but it is necessary to eat more than 0.03% of the adjusted dough. There was a certain thing.

Claims (9)

おからを水分、PH、糖類等の調整をし、準嫌気的雰囲気下で酵母醗酵させて作る醗酵飼料の製造方法。A method for producing fermented feed made by adjusting the moisture, pH, sugars, etc. of okara and fermenting yeast in a semi-anaerobic atmosphere. おからに濃厚飼料又は糟糠類のミールを加える事により、醗酵生地の水分調整を行い生地の含水率を60〜70%に調整する事を特徴とする[請求項1]に記載の醗酵飼料の製造方法。The fermented feed according to claim 1, wherein the moisture content of the fermented dough is adjusted by adding a concentrated feed or rice bran meal to the okara to adjust the moisture content of the dough to 60-70%. Production method. 調整生地のPHを蟻酸又はフマル酸を用いて4.0〜6.0に調整する[請求項1]に記載の醗酵飼料の製造方法。The method for producing a fermented feed according to claim 1, wherein the pH of the adjusted dough is adjusted to 4.0 to 6.0 using formic acid or fumaric acid. ブドウ糖を調整生地に対して0.1〜2重量%添加し、酵母菌の資源とする[請求項1]に記載の醗酵飼料の製造方法。The method for producing a fermented feed according to claim 1, wherein glucose is added in an amount of 0.1 to 2% by weight based on the adjusted dough and used as a yeast resource. ドライイースト菌を調整生地に対し0.1〜2重量%用いてなる[請求項1]に記載の醗酵飼料の製造方法。The method for producing a fermented feed according to claim 1, wherein 0.1-2% by weight of dry yeast is used with respect to the adjusted dough. 高感度甘味料のサツカリンを調整生地に対し0.005〜0.05重量%加えてなる[請求項1]に記載の醗酵飼料の製造方法。The method for producing a fermented feed according to [1], wherein a high-sensitivity sweetener, Satsukarin, is added to the adjusted dough in an amount of 0.005 to 0.05% by weight. 防黴剤のプロピオン酸カルシウムを調整生地に対し、0.1〜1.5重量%加えてなる[請求項1]に記載の醗酵飼料の製造方法。The method for producing a fermented feed according to claim 1, wherein 0.1 to 1.5% by weight of calcium propionate as an antifungal agent is added to the adjusted dough. 醗酵装置は内装容器入りのフレコンバック又はビニール袋を使用する事を特徴とする請求項1記載の醗酵飼料の製造方法。The method for producing a fermented feed according to claim 1, wherein the fermentation apparatus uses a flexible container bag or a plastic bag in an interior container. 醗酵期間は醗酵温度が25℃〜35℃で4日、5℃〜25℃で、凡そ14日であることを特徴とする[請求項1]に記載の醗酵飼料の製造方法。The method for producing a fermented feed according to claim 1, wherein the fermentation period is 4 days at 25 ° C to 35 ° C and about 14 days at 5 ° C to 25 ° C.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110810622A (en) * 2018-08-14 2020-02-21 张金岳 Method for producing biological protein fermentation-fermented feed by applying small molecular kinetic water and special fermentation-fermented bag

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110810622A (en) * 2018-08-14 2020-02-21 张金岳 Method for producing biological protein fermentation-fermented feed by applying small molecular kinetic water and special fermentation-fermented bag

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