JP2013094097A - Non-alcoholic beverage containing eudesmol - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a functional beverage that is a non-alcoholic beverage and has a fresh taste with an excellent cold feeling using a simple and cost-effective means, and that has health functions under mild conditions as a health drink from health-conscious standpoints.SOLUTION: The non-alcoholic beverage having an excellent cold feeling, an autonomic control action, and an alcohol content of less than 1% contains one or more of eudesmols selected from α-eudesmol, β-eudesmol and γ-eudesmol as effective components, with the content of the effective components being 5-100 ppb as the total content of eudesmols. An autonomic control agent contains one or more of eudesmols selected from α-eudesmol, β-eudesmol and γ-eudesmol as effective components, with the content of the effective components being 5-100 ppb as the total content of eudesmols.

Description

  The present invention comprises an alcohol having an autonomic nervous control action and an excellent cooling feeling, containing one or more of udesmol consisting of α-eudesmol, β-eudesmol and γ-eudesmol as an active ingredient. The present invention relates to a non-alcoholic beverage having a content of less than 1% and an autonomic nervous regulator comprising the udesmol as an active ingredient.

  In recent years, in the field of beverages, beverages imparted with various health functions, beverages imparted with various flavors and tastes using various additives from the viewpoint of diversification of tastes or health consciousness. Is provided. With regard to imparting a flavor of “coolness” in beverages, for example, in the production of fruit juice-containing beverages, a refreshing material such as menthol, menthone, camphor, mint oil, peppermint oil, and 3-l-mentoxypropane-1, Disclosed is a refreshing fruit juice-containing beverage with a “cool feeling” and “cool feeling” by adding a cooling sensation substance such as 2-diol and N-ethyl-p-menthane-3-carboxamide. (Japanese Patent Laid-Open No. 2005-143461). In this way, in soft drinks, there has conventionally been a means for intentionally imparting a cold impression around the throat when drinking, which is described by the expression `` sooty '' by using a synthetic fragrance such as menthol as an additive. Has been done.

  In addition, in fermented alcoholic beverages such as beer, a fermented alcoholic beverage excellent in coolness is produced by devising the fermentation method and component ratio and adding a specific amount of β-eudesmol, limonene, α-terpineol. A method is disclosed (Japanese Patent Laid-Open No. 2010-63431).

  However, in the fermented alcoholic beverages as described above, various components adjusted by fermentation methods and device ratios are non-alcoholic beverages that are not affected by the production or metabolism of various substances by fermentation. In the case where it is contained as a beverage component, what kind of flavor is imparted and whether functions such as health can be imparted have not been studied so far.

Japanese Patent Laying-Open No. 2005-143461. Japanese Patent Application Laid-Open No. 2010-63431.

  An object of the present invention is to provide a refreshing taste beverage excellent in coolness by a simple and cost-effective means in a non-alcoholic beverage, and moreover, from a health-oriented viewpoint, it is mild as a health beverage. It is to provide a functional beverage that exhibits health functions under certain conditions.

  In the present invention, in order to solve the above-mentioned problems, in the non-alcoholic beverage, in the earnest study on the provision of a refreshing taste beverage excellent in coolness and the provision of health function in the beverage, the alcohol content is In non-alcoholic beverages such as less than 1%, it is excellent in cool feeling by containing a specific amount of Eudesmol such as α-eudesmol, β-eudesmol and γ-eudesmol in the beverage. It is possible to provide a non-alcoholic beverage having a refreshing taste, and at the same time, it has been found that a health-functional beverage having an autonomic nerve regulation function can be provided by the autonomic nerve regulating action of the component, The present invention has been completed.

That is, the present invention includes one or more of Udesmol consisting of α-eudesmol, β-eudesmol and γ-eudesmol as an active ingredient, and the content of the active ingredient contains udesmol. A non-alcoholic beverage having an alcohol content of less than 1%, which has an autonomic nerve regulating action and has a cooling sensation characterized by a total content of 5 to 100 ppb, or
One or more of udesmol consisting of α-eudesmol, β-eudesmol and γ-eudesmol is an active ingredient, and the total content of udesmol contained in the content of the active ingredient It consists of an autonomic nerve regulator characterized by being 5-100 ppb.

  Eudesmol to be contained in the non-alcoholic beverage of the present invention imparts a cooling sensation to the beverage and imparts an autonomic nerve regulating action to the beverage. The autonomic nervous system that controls autonomic nerve regulation is a system that unconsciously regulates the activity of organs to correspond to the external environment and internal environment, regardless of conscious control. This autonomic nervous system is composed of a sympathetic nervous system in which activity increases in a tension state and a parasympathetic nervous system in which activity increases in a relaxed state. These two types of autonomic nervous system act in an antagonistic manner in response to changes in the external environment such as temperature and mental stress and changes in the internal environment such as nutritional state, and make the body respond to these changes. If you continue to be exposed to excessive stress, the autonomic nerve balance is lost, the sympathetic nerve is excessively active, and the sympathetic nerve activity becomes dominant over the parasympathetic nerve activity. It is said that autonomic ataxia represented by is caused. Therefore, in the modern society where stress is often felt, the autonomic nervous control function is provided as a safe and simple means for maintaining normal autonomic balance, suppressing excessive sympathetic excitation, and increasing parasympathetic activity. The beverage can function effectively from the viewpoint of health function.

  The present invention provides a refreshing tasting beverage excellent in coolness in a non-alcoholic beverage, and the non-alcoholic beverage of the present invention has an autonomic nervous control action, and the present invention is mild as a health beverage. In order to provide functional drinks that exhibit health functions.

FIG. 1 shows GVNA activity when 0.5% CMC aqueous solution containing β-eudesmol 5, 50, or 500 ppb, or 0.5% CMC water was intragastrically administered to 1 ml / 300 g body weight. It is a figure. Each group was tested at n = 3. The value is shown as GVNA neural activity before sample administration (0 min) as 100%. *** indicates that a significant difference was observed. (P <0.0005, ANOVA with repeated measures) FIG. 2 shows ASNA activity when 0.5% CMC aqueous solution containing β-eudesmol 5, 50, or 500 ppb or 0.5% CMC water was intragastrically administered to 1 ml / 300 g body weight. It is a figure. Each group was tested at n = 3. The value shows ASNA nerve activity before sample administration (0 min) as 100%. *** indicates that a significant difference was observed. (P <0.0005, ANOVA with repeated measures)

  The present invention contains one or two or more of Udesmol consisting of α-eudesmol, β-eudesmol and γ-eudesmol as an active ingredient, and the content of udesmol contained in the content of the active ingredient Non-alcoholic beverages having an alcohol content of less than 1% with an autonomic nervous regulation effect of 5 to 100 ppb in total and less than 1%, and α-eudesmol, β-eudesmol and γ-eu It consists of an autonomic nerve regulating agent having 5 to 100 ppb in total of the content of udesmol containing 1 or 2 or more of udesmol consisting of desmole as the active ingredient.

  As the active ingredient in the non-alcoholic beverage of the present invention, α-eudesmol, β-eudesmol, or γ-eudesmol may be commercially available, from natural products and synthetic fragrances containing these. You may use what was refine | purified. Moreover, you may use the natural product containing these, a synthetic | combination fragrance | flavor, and the extract from this natural product or a synthetic | combination fragrance | flavor as a content of (alpha) -eudesmol, (beta) -eudesmol, or (gamma) -eudesmol.

  Examples of natural products used for extraction include hops, which are plants belonging to the mulberry family perennial plants (Huplus luplus), and Eucalyptus globulus, which is an evergreen tree of the Myrtaceae family. These plants may be used as they are, or may be used as treated products obtained by physicochemical treatment or biological treatment of the plants. Examples of plant bodies of these plants include leaves and spikelets for hops, and spikelets are preferably used. You may use the lupulin part of the flower. Examples of hops include German health brooker species, German sparto select species, and Chieco-Saerts species, and since they contain a high content of β-eudesmol, German health brooker species are preferably used. Eucalyptus tree (eucalyptus) includes leaves, twigs, flowers or fruits, but leaves are preferably used.

  Examples of the physicochemical treatment of the plant include drying treatment by sun drying, ventilation drying, freeze drying, etc., pulverization treatment by a blender, a homogenizer, a ball mill and the like. A pulverized processed material etc. are mentioned. Examples of biological treatment include fermentation treatment with bacteria, yeast and the like, and examples of biological treatment products include fermentation treatment products. As an example of the processed material of a plant body, the hop pellet which is a compression product of a hop camellia is mentioned, for example. Examples of the plant extract of the present invention include extracts that can be obtained from the plant by a method of extracting a substance from the plant, such as solvent extraction, supercritical fluid extraction, steam distillation, etc., but solvent extraction is preferred. .

  As the solvent used for the solvent extraction, if the solvent can extract α-eudesmol, β-eudesmol, or γ-eudesmol from the plant used in the present invention or a processed product thereof, for example, water, An aqueous medium such as purified water, deionized water, or distilled water, or an organic solvent such as alcohol, alkyl acetate, aliphatic ketone, aliphatic ether, aliphatic hydrocarbon, or alicyclic hydrocarbon may be used. Alcohol is preferred, and ethanol is more preferably used. A solvent may be used independently and may be used as a mixed solvent which combined two or more.

  For the solvent extraction, equipment used in usual solvent extraction, such as a stirrer, an ultrasonic generator, a reflux extractor, a Soxhlet extractor, or the like is used. The amount of solvent used for solvent extraction is not particularly limited, but for example, 0.1 to 10,000 parts by weight, preferably 1 to 1000 parts by weight, and more preferably 5 to 100 parts by weight of solvent with respect to 1 part by weight of the plant. To do. The extraction temperature is not particularly limited as long as the temperature is not lower than the melting point and not higher than the boiling point of the solvent, but is preferably 0 to 100 ° C in an aqueous medium, more preferably 20 to 80 ° C, and preferably 0 to 1000 ° C in an organic solvent. 20-80 degreeC is more preferable.

  The extraction time is not particularly limited, but is preferably 1 minute to 1 year, more preferably 30 minutes to 1 week. After the solvent extraction is completed, the obtained extract is subjected to sedimentation separation, cake filtration, clarification filtration, centrifugal filtration, centrifugal sedimentation, compression, separation, filter press and the like, preferably by filtration, An extract may be obtained and used as an extract. Further, the extraction residue obtained by the solid-liquid separation method may be further extracted with an extraction solvent, and this may be used as an extract. An extract obtained from a plant by an extraction method such as solvent extraction or supercritical fluid separation may be further processed using a solid-liquid separation method, a concentration or drying method, a purification method, or the like.

  As means for obtaining an extract from a plant body, hop essential oil, hop extract, eucalyptus essential oil, eucalyptus extract, etc. are commercially available and contain α-eudesmol, β-eudesmol, or γ-eudesmol. As long as it does, you may use these commercial items. After the above extract is dissolved in a solvent as required, α-eudesmol, β in the extract using a purification method such as a membrane separation method, a liquid membrane separation method, a solvent distribution method, or a fractionation method. -The concentration of udesmol or γ-eudesmol may be increased or unnecessary substances may be removed.

In preparing the plant extract of the present invention, for example, an antioxidant, a preservative, etc. are added and heated in order not to inactivate α-eudesmol, β-eudesmol, or γ-eudesmol. Adjustment of temperature etc. may be made. In the beverage of the present invention, α-eudesmol, β-eudesmol, or γ-eudesmol, or a content thereof, at the time of production or after production thereof, these contents in the beverage are 5 to 100 ppb in total. Except adding so that it may become, it can manufacture with the normal manufacturing method of the non-alcoholic drink (beverage whose alcohol content is less than 1%) of a drink.
The content of α-eudesmol, β-eudesmol and γ-eudesmol in the beverage of the present invention can be quantified by a conventional method such as using a commercially available GC / MS apparatus.

  Examples of the non-alcoholic beverage of the present invention include mineral water, near water, sports drink, tea beverage, milk beverage, coffee beverage, fruit juice beverage, vegetable juice beverage, fruit juice and vegetable juice beverage, and carbonated beverage. However, it is not limited to these. It may be a beer beverage having an alcohol content of less than 1%, such as non-alcohol beer. Mineral water includes both foaming and non-foaming mineral water.

Tea drinks are tea leaves that are evergreens of the camellia family (tea leaves), or drinks for roasting and drinking leaves or cereals of plants other than tea trees, such as fermented tea, semi-fermented tea, and non-fermented tea Both are included. Specific examples of tea beverages include Japanese tea (for example, green tea, barley tea), black tea, herbal tea (for example, jasmine tea), Chinese tea (for example, Chinese green tea, oolong tea), hoji tea, and the like.
Milk beverages refer to beverages made mainly from raw milk, milk, etc. or foods made from these raw materials. In addition to those made of milk and other ingredients, for example, nutrient-enriched milk, flavored milk, and sugar-decomposed milk Those made from processed milk are also included.

  Examples of fruits used in fruit juice-containing beverages and fruit and vegetable juice-containing beverages include apples, mandarin oranges, grapes, bananas, pears, peaches, mangoes, and the like. Examples of vegetables used in vegetable juice-containing beverages, fruit juices, and vegetable juice-containing beverages include tomato, carrot, celery, pumpkin, celery, and cucumber.

  The present invention provides a non-alcoholic beverage having an excellent cooling feeling and a health function having an autonomic nerve control function, but also provides an autonomic nerve regulator having an autonomic nerve control function. When the beverage of the present invention is ingested as an autonomic nervous regulator, one adult takes 50 μg to 5 μg, preferably 50 μg per day as the total amount of α-eudesmol, β-eudesmol and γ-eudesmol. It is preferable to ingest so that it may be-3 micrograms, More preferably, it may be 50 micrograms-2.5 micrograms.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated further more concretely, this invention is not limited to these Examples.

  An aqueous solution containing 2.5 ppb, 5 ppb, 10 ppb, and 50 ppb of β-eudesmol, respectively, was prepared. An aqueous solution not containing β-eudesmol was used as a control, and whether or not it felt cooler than the control was evaluated blindly by 8 panelists.

  As a result, 4 people in an aqueous solution containing 2.5 ppb of β-eudesmol, 6 people in an aqueous solution containing 5 ppb of β-eudesmol, 7 people in an aqueous solution containing 10 ppb of β-eudesmol, β-eudesmol In an aqueous solution containing 50 ppb of mole, all eight responded that the aqueous solution containing β-eudesmol felt cooler than water.

  A Wistar male rat (about 9 weeks old) with a body weight of about 300 g kept in a constant temperature animal room at 24 ° C. under a light-dark cycle every 12 hours (lighted from 8 to 20) was fasted for 3 hours. . After fasting, anesthetized with urethane, inserted into a cannula for intragastric administration, and the gastric vagus nerve or adrenal sympathetic nerve centrifuge branch, a parasympathetic nerve, was lifted with a silver electrode, and the electrical activity of these nerves was measured (Shen J, et al. al. Neurosci. Lett. 383188-193, 2005; Tanida M, et al., Neurosci. Lett. 389: 109-114, 2005).

  On the other hand, 0.5% carboxymethylcellulose (CMC) aqueous solution (hereinafter referred to as β-eudesmol CMC aqueous solution) containing 5, 50, or 500 ppb of β-eudesmol, respectively, was prepared. When these measured values settled, 1.0 ml / 300 g body weight of each β-eudesmol CMC aqueous solution was intragastrically administered, and changes in the electrical activity of these gastric vagus nerve or adrenal sympathetic nerve centrifuge branch occurred at that time Was electrophysiologically measured for 90 minutes. The tube was inserted into the trachea from the start of surgery to the end of measurement to secure the airway, and the body temperature (rat rectal temperature) was maintained at 35.0 ± 0.5 ° C. with a heat retaining device. Further, the same operation was performed except that a CMC aqueous solution containing no β-eudesmol was used as a control, and this was used as a control administration group.

  The data of these neural activities are analyzed by the average value of the firing frequency per 5 seconds (pulse / 5s) every 5 minutes, and expressed as a percentage with the average value (0 minute value) for 5 minutes before the start of stimulation as 100%. did. In addition, the mean value + standard error is calculated from the data, and the statistical significance of the group is tested by analysis of variance (ANOVA) with repeated measures, and the neuronal activity before the start of intragastric administration (0 minutes). The test of statistical significance between absolute values was performed by Mann-Whitney U-test.

  The results for gastric vagal nerve activity (GVNA) are shown in FIG. 1, and the results for adrenal sympathetic nerve activity (ASNA) are shown in FIG.

As shown in FIG. 1, in the group in which the β-eudesmol CMC aqueous solution was intragastrically administered, GVNA was maintained at a higher level than the control administration group at any concentration of β-eudesmol. In particular, administration at 5 ppb maintained the highest GVNA activity.
When the GVNA value between 5 minutes and 90 minutes after the start of intragastric administration was compared between the group administered with the β-eudesmol CMC aqueous solution and the control administration group by statistical examination, β-eudesmol The GVNA value of the group administered with the CMC aqueous solution was significantly higher than the GVNA value of the control administration group. There was no statistically significant difference between absolute values of neural activity before the start of intragastric administration (0 minutes).

  Further, as shown in FIG. 2, in the group administered with the β-eudesmol CMC aqueous solution, ASNA after 90 minutes of administration was at a lower level than the control administration group at any concentration of β-eudesmol. Met. In particular, the group administered with 5 ppb of β-eudesmol CMC aqueous solution always had a lower value than the control administration group from 5 minutes to 90 minutes after the administration. When the ASNA value between 5 minutes and 90 minutes after the start of intragastric administration was compared between the group administered with the β-eudesmol CMC aqueous solution and the control group by statistical examination, 5 ppb or 50 ppb β -The ASNA value of the group administered with the Udesmol CMC aqueous solution was significantly lower than the ASNA value of the control administration group. Since there was no statistically significant difference between the absolute values of nerve activity before the start of intragastric administration (0 minutes), it was considered that there was no effect due to individual differences in the animals used in the study.

  The effects shown above, ie, the effect of increasing GVNA, which is one of parasympathetic nerve activities, and the effect of decreasing ASNA, which is one of sympathetic nerve activities, are that β-eudesmol has an autonomic nerve regulating action. Is shown.

A beverage (1 L) is produced by the conventional method with the following composition. Udesmol perfume is prepared by perfume extracts from plants containing udesmol:
Fructose glucose liquid sugar 40g
(Fructose content 55%)
Citric acid (anhydrous) 1.1g
Trisodium citrate (crystal) 0.4g
Udes Mall Fragrance 0.05g
(Contains 5 ppb β-eudesmol as final concentration)
Water proper amount total 1000g

The present invention provides a refreshing taste beverage excellent in coolness by a simple and cost-effective means in a non-alcoholic beverage, and the non-alcoholic beverage of the present invention has an autonomic nerve regulating action, The invention provides a functional beverage that exhibits a health function under mild conditions as a health beverage.

That is, the present invention comprises: (1) one or more udesmol selected from α-eudesmol, β-eudesmol, and γ-eudesmol as an active ingredient, and the content of the active ingredient is 5 to 5 A non-alcoholic beverage having an alcohol content of less than 1% and having an autonomic nervous control effect characterized by an autonomic nervous regulation action of 100 ppb, and (2) active ingredients are α-eudesmol and β-eudes A non-alcoholic beverage having an autonomic nerve-regulating action and having a cooling sensation excellent in cooling sensation, wherein the alcohol content is less than 1%, and (3) Udesmol is a hop or A non-alcohol with an alcohol content of less than 1%, which has an autonomic nerve regulating action and has a cooling sensation as described in (1) or (2) above, which is a component extracted from Eucalyptus Or (4) a non-alcoholic beverage having an alcohol content of less than 1% is a tea beverage, a carbonated beverage, or a non-alcoholic beer beverage. A non-alcoholic beverage with an alcohol content of less than 1% having an autonomic nervous control effect and an alcohol content of less than 1%, or (5) a non-alcoholic beverage with an alcohol content of less than 1%, A non-alcoholic beverage having an autonomic nerve-regulating action according to any one of the above (1) to (3), wherein the alcohol content is less than 1%, and (6) alcohol A container-packed beverage consisting of a non-alcoholic beverage having a content of less than 1% is prepared so that the daily intake is 50 μg to 2.5 μg in the total amount of active ingredient Eudesmol. A non-alcoholic beverage having an autonomic nerve-regulating action as described in the above (5) and having an alcohol content of less than 1% and (7) a Udes described in the above (1) the mall as an active ingredient, consisting of autonomic modulating agent, wherein the amount of the active ingredient is 5~100ppb a total content of U des mall containing.

That is, this invention was excellent in the cool feeling which has the autonomic nerve adjustment effect | action characterized by using (1) ( beta ) -eudesmol as an active ingredient , and content in the drink of this active ingredient is 5-100ppb. The non-alcoholic beverage having an alcohol content of less than 1%, or (2 ) β- eudesmol is a component extracted from hops or eucalyptus, and described in ( 1 ) above. non or alcoholic beverages alcohol content with excellent cool feel is less than 1% with, (3) non-alcoholic beverages alcohol content is less than 1%, tea drinks, carbonated drinks, or non-alcoholic beer beverages A non-alcoholic beverage with an alcohol content of less than 1%, which has an autonomic nerve-regulating action and is excellent in cooling sensation as described in ( 1) or (2 ), 4 ) A non-alcoholic beverage having an alcohol content of less than 1% is a container-packed beverage, and is excellent in cooling feeling having an autonomic nerve regulating action according to any one of (1) to (3) above In a total amount of β- eudesmol, which is a non-alcoholic beverage having an alcohol content of less than 1%, and ( 5 ) a non-alcohol beverage having an alcohol content of less than 1%, The amount of alcohol consumed per day is adjusted to 50 μg to 2.5 μg, and the alcohol content with an autonomic nerve regulating action according to the above ( 4 ) having an excellent cooling feeling is less than 1% non-alcoholic beverages or Ranaru is.

Claims (2)

  One or more of udesmol consisting of α-eudesmol, β-eudesmol and γ-eudesmol is an active ingredient, and the total content of udesmol contained in the content of the active ingredient A non-alcoholic beverage with an alcohol content of less than 1%, which has an autonomic nerve regulating action and is excellent in cooling, characterized by being 5 to 100 ppb. One or more of udesmol consisting of α-eudesmol, β-eudesmol and γ-eudesmol is an active ingredient, and the total content of udesmol contained in the content of the active ingredient Autonomic nerve regulator characterized by being 5-100ppb.