WO2013065224A1 - Non-alcoholic beverage containing eudesmol - Google Patents

Abstract

The present invention addresses the problem of providing a non-alcoholic beverage, which shows an excellent cool and refreshing taste and can be obtained by a simple and cost-saving method, and, from the view point of health promotion, which is a functional beverage capable of exerting a health function as a health beverage under mild conditions, and a drug with a health function which comprises, as the active component, a component of the aforesaid functional beverage. Provided is a non-alcoholic beverage, which has an autonomic nerve modulating effect, shows an excellent cool taste and has an alcohol content of less than 1%, characterized by comprising, as the active component, one or more kinds of eudesmols selected from among α-eudesmol, β-eudesmol and γ-eudesmol and the content of the active component being 5-100 ppb. Also provided is an autonomic nerve modulating agent characterized by comprising, as the active component, the eudesmol(s) contained in the aforesaid beverage and the content of the active component, in terms of the total content of the eudesmol(s) therein, being 5-100 ppb.

Description

Non-alcoholic beverages containing udesmol

The present invention relates to an alcohol having an autonomic nerve-regulating action and having an excellent cooling sensation, comprising as an active ingredient one or more of udesmol consisting of α-eudesmol, β-eudesmol, and γ-eudesmol The present invention relates to a non-alcoholic beverage having a content of less than 1% and an autonomic nervous regulator comprising the udesmol as an active ingredient.

In recent years, in the field of beverages, beverages imparted with various health functions, beverages imparted with various flavors and tastes using various additives from the viewpoint of diversification of tastes or health consciousness. Is provided. With regard to imparting a flavor of “coolness” in beverages, for example, in the production of fruit juice-containing beverages, a refreshing material such as menthol, menton, camphor, mint oil, peppermint oil, and 3-l-menthoxypropane-1, Disclosed is the production of a refreshing fruit juice-containing beverage with a “cool feeling” and “cool feeling” by adding a cooling sensation substance such as 2-diol and N-ethyl-p-menthane-3-carboxamide. (Japanese Patent Laid-Open No. 2005-143461). In this way, in soft drinks, there has conventionally been a means for intentionally imparting a cold impression around the throat when drinking, which is described by the expression `` sooty '' by using a synthetic fragrance such as menthol as an additive. Has been done.

In addition, in fermented alcoholic beverages such as beer, the fermented alcoholic beverage with excellent coolness is produced by devising the fermentation method and component ratio and adding a specific amount of β-eudesmol, limonene, and α-terpineol. A method is disclosed (Japanese Patent Laid-Open No. 2010-63431).

However, in the fermented alcoholic beverages as described above, various components adjusted by fermentation methods and device ratios are non-alcoholic beverages that are not affected by the production or metabolism of various substances by fermentation. In the case where it is contained as a beverage component, what kind of flavor is imparted and whether functions such as health can be imparted have not been studied so far.

Japanese Patent Application Laid-Open No. 2005-143461. JP 2010-63431 A.

The object of the present invention is to provide a refreshing taste beverage excellent in coolness by means that are simple and cost-effective in non-alcoholic beverages, and from a health-oriented viewpoint, under mild conditions as a health beverage, The object is to provide a functional beverage that exhibits health functions. Furthermore, the subject of this invention is providing the chemical | medical agent for a health function which uses the component of this functional drink as an active ingredient.

In the present invention, in order to solve the above-mentioned problems, in the non-alcoholic beverage, in the earnest study on the provision of a refreshing taste beverage excellent in coolness and the provision of health function in the beverage, the alcohol content is In non-alcoholic beverages such as less than 1%, it is excellent in cool feeling by containing a specific amount of Eudesmol such as α-eudesmol, β-eudesmol and γ-eudesmol in the beverage. It is possible to provide a non-alcoholic beverage having a refreshing taste, and at the same time, it has been found that a health-functional beverage having an autonomic nerve regulation function can be provided by the autonomic nerve regulating action of the component, The present invention has been completed.

That is, in the present invention, one or more udesmol selected from α-eudesmol, β-eudesmol, and γ-eudesmol is an active ingredient, and the content of the active ingredient is 5 to 100 ppb. It consists of a non-alcoholic drink with an alcohol content of less than 1%, which has an autonomic nerve control action and is excellent in coolness. In the present invention, a preferred combination of udesmol may include a combination of α-eudesmol and β-eudesmol.

The Eudesmol, which is an active ingredient of the present invention, can be appropriately obtained as an extracted component from the component-containing plant and the like, and preferred plants include hops and eucalyptus.

In the present invention, examples of non-alcoholic beverages having an alcohol content of less than 1% include tea beverages, carbonated beverages, and non-alcoholic beer beverages. The non-alcoholic beverage can be prepared as a container-packed beverage. The packaged beverage can be prepared as a product prepared such that the daily intake is 50 μg to 2.5 μg in the total amount of udesmol as an active ingredient.

The present invention comprises one or more udesmol selected from α-eudesmol, β-eudesmol, and γ-eudesmol as an active ingredient, It can be provided as an autonomic nerve regulator characterized by a total content of 5 to 100 ppb.

The Udes Mall contained in the non-alcoholic beverage of the present invention imparts a cooling sensation to the beverage and imparts an autonomic nerve regulating action to the beverage. The autonomic nervous system that controls autonomic nerve regulation is a system that unconsciously regulates the activity of organs to correspond to the external environment and internal environment, regardless of conscious control. This autonomic nervous system is composed of a sympathetic nervous system in which activity increases in a tension state and a parasympathetic nervous system in which activity increases in a relaxed state. These two types of autonomic nervous system act in an antagonistic manner in response to changes in the external environment such as temperature and mental stress and changes in the internal environment such as nutritional state, and make the body respond to these changes. If you continue to be exposed to excessive stress, the autonomic nerve balance is lost, the sympathetic nerve is excessively active, and the sympathetic nerve activity becomes dominant over the parasympathetic nerve activity. It is said that autonomic ataxia represented by is caused. Therefore, in the modern society where stress is often felt, the autonomic nervous control function is provided as a safe and simple means for maintaining normal autonomic balance, suppressing excessive sympathetic excitation, and increasing parasympathetic activity. The beverage can function effectively from the viewpoint of health function.

Specifically, the present invention includes (1) containing one or more udesmol selected from α-eudesmol, β-eudesmol and γ-eudesmol as an active ingredient, A non-alcoholic beverage with an alcohol content of less than 1% having an autonomic nerve control action and an alcohol content of less than 1%, characterized in that the amount is 5 to 100 ppb, and (2) the active ingredient is α-eudesmol and A non-alcoholic beverage having an autonomic nerve-regulating action and having a cooling sensation excellent in cooling feeling and having an alcohol content of less than 1%, characterized by comprising β-eudesmol, and (3) udesmol Is an ingredient extracted from hops or eucalyptus, the alcohol content having an autonomic nerve regulating action as described in the above (1) or (2) and having an excellent cooling feeling is less than 1% It consists of a non-alcoholic beverage.

In the present invention, (4) the non-alcoholic beverage having an alcohol content of less than 1% is a tea beverage, a carbonated beverage, or a non-alcoholic beer beverage. A non-alcoholic beverage having an autonomic nerve-regulating action and an alcohol content that is less than 1% and (5) a non-alcoholic beverage having an alcohol content of less than 1% A non-alcoholic beverage having an autonomic nerve regulating action and having a cooling sensation excellent in cooling feeling according to any one of the above (1) to (3), wherein the alcohol content is less than 1%, (6) Container-packed beverages consisting of non-alcoholic beverages with an alcohol content of less than 1% are adjusted so that the daily intake is 50 μg to 2.5 μg in the total amount of active ingredient Eudesmol. A non-alcoholic beverage having an alcohol content of less than 1% having an autonomic nerve regulating action as described in (5) above, wherein the alcohol content is less than 1%, and (7) the item as described in (1) above, It consists of an autonomic nervous regulator characterized by containing udesmol as an active ingredient, and the total content of udesmol contained in the active ingredient is 5 to 100 ppb.

The present invention provides a refreshing tasting beverage excellent in coolness in a non-alcoholic beverage, and the non-alcoholic beverage of the present invention has an autonomic nervous control action, and the present invention is mild as a health beverage. In order to provide functional drinks that exhibit health functions.

FIG. 1 shows GVNA activity when 0.5% CMC aqueous solution containing β- eudesmol 5,50, or 500 ppb or 0.5% CMC water was intragastrically administered to 1 ml / 300 g body weight. It is a figure. Each group was tested at n = 3. The value is shown as GVNA neural activity before sample administration (0 min) as 100%. *** indicates that a significant difference was observed. (P <0.0005, ANOVA with repeated measures) FIG. 2 shows ASNA activity when 0.5% CMC aqueous solution containing β- eudesmol 5, 50, or 500 ppb or 0.5% CMC water was intragastrically administered to 1 ml / 300 g body weight. It is a figure. Each group was tested at n = 3. The value shows ASNA nerve activity before sample administration (0 min) as 100%. *** indicates that a significant difference was observed. (P <0.0005, ANOVA with repeated measures)

The present invention comprises one or more of udesmol consisting of α-eudesmol, β-eudesmol and γ-eudesmol as an active ingredient, and the content of udesmol contained in the content of the active ingredient Non-alcoholic beverages with an alcohol content of less than 1% having an autonomic nervous regulation effect of 5 to 100 ppb in total and an alcohol content of less than 1%, and α-eudesmol, β-eudesmol and γ-eu It consists of an autonomic nervous regulator that contains one or more of Udesmol consisting of Desmol as an active ingredient, and the total content of Udesmol contained in the active ingredient is 5 to 100 ppb.

As the active ingredient in the non-alcoholic beverage of the present invention, α-eudesmol, β-eudesmol, or γ-eudesmol may be commercially available, from natural products or synthetic fragrances containing them. You may use what was refine | purified. In addition, natural products containing these, synthetic fragrances, and extracts from the natural products or synthetic fragrances may be used as inclusions of α-eudesmol, β-eudesmol, or γ-eudesmol.

Examples of natural products used for extraction include hops that are plants belonging to the mulberry perennial plant (Huplus luplus), Eucalyptus globulus, which is an evergreen tree of the Myrtaceae family, and the like. These plants may be used as they are, or may be used as treated products obtained by physicochemical treatment or biological treatment of the plants. Examples of plant bodies of these plants include leaves and spikelets for hops, and spikelets are preferably used. You may use the lupulin part of the flower. Hops include German health brooker species, German sparte select species, and Chieco Saz varieties. Since β-eudesmol is highly contained, German health brooker species are preferably used. Eucalyptus tree (eucalyptus) includes leaves, twigs, flowers or fruits, but leaves are preferably used.

Examples of the physicochemical treatment of the plant include drying treatment by sun drying, ventilation drying, freeze drying, etc., pulverization treatment by a blender, a homogenizer, a ball mill and the like. A pulverized processed material etc. are mentioned. Examples of biological treatment include fermentation treatment with bacteria, yeast and the like, and examples of biological treatment products include fermentation treatment products. As an example of the processed material of a plant body, the hop pellet which is a compression product of a hop camellia is mentioned, for example. Examples of the plant extract of the present invention include extracts that can be obtained from the plant by a method of extracting a substance from the plant, such as solvent extraction, supercritical fluid extraction, steam distillation, etc., but solvent extraction is preferred. .

The solvent used for solvent extraction is, for example, water, as long as it can extract α-eudesmol, β-eudesmol, or γ-eudesmol from the plant used in the present invention or a processed product thereof. An aqueous medium such as purified water, deionized water, or distilled water, or an organic solvent such as alcohol, alkyl acetate, aliphatic ketone, aliphatic ether, aliphatic hydrocarbon, or alicyclic hydrocarbon may be used. Alcohol is preferred, and ethanol is more preferably used. A solvent may be used independently and may be used as a mixed solvent which combined two or more.

For the solvent extraction, equipment used in ordinary solvent extraction, such as a stirrer, an ultrasonic generator, a reflux extractor, a Soxhlet extractor, or the like is used. The amount of the solvent used for the solvent extraction is not particularly limited, but for example, 0.1 to 10000 parts by weight, preferably 1 to 1000 parts by weight, more preferably 5 to 100 parts by weight of the solvent per 1 part by weight of the plant body To do. The extraction temperature is not particularly limited as long as the temperature is not lower than the melting point and not higher than the boiling point of the solvent, but is preferably 0 to 100 ° C., more preferably 20 to 80 ° C. for an aqueous medium, and preferably 0 to 1000 ° C. for an organic solvent. 20 to 80 ° C. is more preferable.

The extraction time is not particularly limited, but is preferably 1 minute to 1 year, more preferably 30 minutes to 1 week. After the solvent extraction is completed, the obtained extract is subjected to sedimentation separation, cake filtration, clarification filtration, centrifugal filtration, centrifugal sedimentation, compression, separation, filter press and the like, preferably by filtration, An extract may be obtained and used as an extract. Further, the extraction residue obtained by the solid-liquid separation method may be further extracted with an extraction solvent, and this may be used as an extract. An extract obtained from a plant by an extraction method such as solvent extraction or supercritical fluid separation may be further processed using a solid-liquid separation method, a concentration or drying method, a purification method, or the like.

As means for obtaining extracts from plants, hop essential oil, hop extract, eucalyptus essential oil, eucalyptus extract, etc. are commercially available and contain α-eudesmol, β-eudesmol, or γ-eudesmol. As long as it does, you may use these commercial items. After the above extract is dissolved in a solvent as necessary, α-eudesmol, β in the extract is purified using a purification method such as membrane separation, liquid membrane separation, solvent partitioning, or fractionation. -The concentration of udesmol or γ-eudesmol may be increased, or unnecessary substances may be removed.

In preparing the plant extract of the present invention, for example, an antioxidant, a preservative, etc. are added and heated so as not to inactivate α-eudesmol, β-eudesmol, or γ-eudesmol. Adjustment of temperature etc. may be made. The beverage of the present invention has a total content of 5 to 100 ppb in the beverage at the time of or after the production of α-eudesmol, β-eudesmol, or γ-eudesmol, or the content thereof. Except adding so that it may become, it can manufacture with the normal manufacturing method of the non-alcoholic drink (beverage whose alcohol content is less than 1%) of a drink.
The contents of α-eudesmol, β-eudesmol and γ-eudesmol in the beverage of the present invention can be quantified by a conventional method such as using a commercially available GC / MS apparatus.

Examples of the non-alcoholic drink of the present invention include mineral water, near water, sports drink, tea drink, milk drink, coffee drink, fruit juice drink, vegetable juice drink, fruit juice and vegetable juice drink, carbonated drink, and the like. However, it is not limited to these. It may be a beer beverage having an alcohol content of less than 1%, such as non-alcohol beer. Mineral water includes both foaming and non-foaming mineral water.

Tea drinks are tea leaves (tea leaves), which are evergreen trees of the camellia family, or drinks for brewing and drinking leaves or cereals of plants other than tea trees. Both are included. Specific examples of tea beverages include Japanese tea (for example, green tea, barley tea), black tea, herbal tea (for example, jasmine tea), Chinese tea (for example, Chinese green tea, oolong tea), hoji tea, and the like.
Milk beverages refer to beverages made mainly from raw milk, milk, etc. or foods made from these raw materials. In addition to those made of milk and other ingredients, for example, nutrient-enriched milk, flavored milk, and sugar-decomposed milk Those made from processed milk are also included.

Examples of fruits used in fruit juice-containing beverages and fruit and vegetable juice-containing beverages include apples, mandarin oranges, grapes, bananas, pears, peaches, and mangoes. Examples of vegetables used in vegetable juice-containing beverages, fruit juices, and vegetable juice-containing beverages include tomato, carrot, celery, pumpkin, celery, and cucumber.

The present invention provides a non-alcoholic beverage having a cooling function and a health function having an autonomic nerve control function, but also provides an autonomic nerve regulator having an autonomic function. When the beverage of the present invention is ingested as an autonomic nervous regulator, one adult is 50 μg to 5 μg, preferably 50 μg per day as the total amount of α-eudesmol, β-eudesmol, and γ-eudesmol. It is preferable to take it to ˜3 μg, more preferably 50 μg to 2.5 μg.

Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples.

An aqueous solution containing 2.5 ppb, 5 ppb, 10 ppb, and 50 ppb of β-eudesmol, respectively, was prepared. An aqueous solution containing no β-eudesmol was used as a control, and whether or not it felt cooler than the control was evaluated blindly by 8 panelists.

As a result, 4 people in the aqueous solution containing 2.5 ppb of β-eudesmol, 6 people in the aqueous solution containing 5 ppb of β-eudesmol, 7 people in the aqueous solution containing 10 ppb of β-eudesmol, β-eudesmol In an aqueous solution containing 50 ppb of mol, all eight responded that the aqueous solution containing β-eudesmol felt cooler than water.

A Wistar male rat (approx. 9 weeks old) with a body weight of about 300 g kept in a constant temperature animal room at 24 ° C. for 12 hours or more under a light / dark cycle every 12 hours (lighted from 8 o'clock to 20 o'clock) was fasted for 3 hours. . After fasting, urethane anesthesia was performed, a cannula for intragastric administration was inserted, and the gastric vagus nerve or adrenal sympathetic nerve centrifuge branch, which was a parasympathetic nerve, was suspended with a silver electrode, and the electrical activity of these nerves was measured (Shen J, et al. Neurosci. Lett. 383188-193, 2005; Tanida M, et al., Neurosci. Lett. 389: 109-114, 2005).

On the other hand, 0.5% carboxymethylcellulose (CMC) aqueous solution (hereinafter referred to as β-eudesmol CMC aqueous solution) containing 5,50 or 500 ppb of β-eudesmol, respectively, was prepared. When these measured values settled, 1.0 ml / 300 g body weight of each β-eudesmol CMC aqueous solution was intragastrically administered, and changes in the electrical activity of these gastric vagus nerve or adrenal sympathetic nerve centrifuge branch that occurred at that time Was electrophysiologically measured for 90 minutes. The tube was inserted into the trachea from the start of surgery to the end of measurement to secure the airway, and the body temperature (rat rectal temperature) was maintained at 35.0 ± 0.5 ° C. with a heat retaining device. Further, the same operation was performed except that a CMC aqueous solution containing no β-eudesmol was used as a control, and this was used as a control administration group.

The data of these nerve activities are analyzed by the average value of the firing frequency (pulse / 5s) per 5 seconds every 5 minutes, and expressed as a percentage with the average value (0 minute value) for 5 minutes before the start of stimulation as 100%. did. In addition to calculating the mean value + standard error from the data, the statistical significance test as a group is performed by analysis of variance (ANOVA) with repeated measures, and the neural activity before the start of intragastric administration (0 min) The test of statistical significance between absolute values was performed by Mann-Whitney U-test.

The results for gastric vagal nerve activity (GVNA) are shown in FIG. 1 and the results for adrenal sympathetic nerve activity (ASNA) are shown in FIG.

As shown in FIG. 1, in the group to which the β-eudesmol CMC aqueous solution was intragastrically administered, GVNA was maintained at a higher level than the control administration group at any concentration of β-eudesmol. In particular, administration at 5 ppb maintained the highest GVNA activity.
When the GVNA value between 5 minutes and 90 minutes after the start of intragastric administration was compared by statistical examination between the group administered with β-eudesmol CMC aqueous solution and the control administration group, β-eudesmol The GVNA value of the group administered with the CMC aqueous solution was significantly higher than the GVNA value of the control administration group. There was no statistically significant difference between absolute values of neural activity before the start of intragastric administration (0 minutes).

In addition, as shown in FIG. 2, in the group administered with β-eudesmol CMC aqueous solution, ASNA after 90 minutes of administration was lower than that in the control administration group at any concentration of β-eudesmol. Met. In particular, the group administered with 5 ppb β-eudesmol CMC aqueous solution always had a lower value than the control group from 5 minutes to 90 minutes after the administration. When the ASNA value between 5 minutes and 90 minutes after the start of intragastric administration was compared between the group administered with the β-eudesmol CMC aqueous solution and the control group by statistical examination, 5 ppb or 50 ppb β -The ASNA value of the group administered with the Eudesmol CMC aqueous solution was significantly lower than the ASNA value of the control group. Since there was no statistically significant difference between the absolute values of nerve activity before the start of intragastric administration (0 minutes), it was considered that there was no effect due to individual differences in the animals used in the study.

The effects shown above, namely, the effect of increasing GVNA, which is one of parasympathetic nerve activities, and the effect of decreasing ASNA, which is one of sympathetic nerve activities, are that β-eudesmol has an autonomic nerve regulating action. Is shown.

A beverage (1 L) is produced by the conventional method with the following composition. Udesmol perfume is prepared by perfume extracts from plants containing udesmol:
Fructose glucose liquid sugar 40g
(Fructose content 55%)
Citric acid (anhydrous) 1.1g
Trisodium citrate (crystal) 0.4g
Udes Mall Fragrance 0.05g
(Contains 5 ppb β-eudesmol as final concentration)
Water proper amount total 1000g

The present invention provides a refreshing taste beverage excellent in coolness by a simple and cost-effective means in a non-alcoholic beverage, and the non-alcoholic beverage of the present invention has an autonomic nerve regulating action, The invention provides a functional beverage that exhibits health functions under mild conditions as a health beverage.

Claims (7)

1. Autonomy characterized in that one or more udesmol selected from α-eudesmol, β-eudesmol and γ-eudesmol is an active ingredient, and the content of the active ingredient is 5 to 100 ppb A non-alcoholic beverage having an alcohol content of less than 1% and having a neuromodulating effect and a cool feeling
2. The non-alcohol with an alcohol content of less than 1% having an autonomic nerve-regulating action and excellent cooling feeling according to claim 1, wherein the active ingredient comprises α-eudesmol and β-eudesmol Beverages.
3. The non-alcohol with an alcohol content of less than 1% having a cooling sensation having an autonomic nervous regulation effect according to claim 1 or 2, wherein udesmol is a component extracted from hops or eucalyptus Beverages.
4. 4. The autonomic nerve regulating action according to claim 1, wherein the non-alcoholic beverage having an alcohol content of less than 1% is a tea beverage, a carbonated beverage or a non-alcoholic beer beverage. A non-alcoholic beverage with an alcohol content of less than 1% with excellent coolness.
5. The non-alcoholic beverage having an alcohol content of less than 1% is a container-packed beverage, wherein the alcohol content having an autonomic nerve regulating action and an excellent cooling feeling is provided. Non-alcoholic beverages that are less than 1%.
6. A container-packed beverage comprising a non-alcoholic beverage having an alcohol content of less than 1% is prepared so that the daily intake is 50 μg to 2.5 μg in the total amount of active ingredient Eudesmol. The non-alcoholic beverage with an alcohol content of less than 1% having excellent cooling feeling and having an autonomic nerve regulating action according to claim 5.
7. An autonomic nervous modulator comprising the udesmol of claim 1 as an active ingredient, and the total content of udesmol contained in the active ingredient is 5 to 100 ppb.
   
   

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